Osteoarthritis and cartilage open最新文献

{"title":"Slow acting medications for progressive and painful knee osteoarthritis. How do we assess the benefit to risk of these potentially novel therapies?","authors":"Nancy E. Lane, Lee S. Simon, Jeyanesh Tambiah","doi":"10.1016/j.ocarto.2024.100546","DOIUrl":"10.1016/j.ocarto.2024.100546","url":null,"abstract":"","PeriodicalId":74377,"journal":{"name":"Osteoarthritis and cartilage open","volume":"7 1","pages":"Article 100546"},"PeriodicalIF":0.0,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11683324/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142908077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MicroRNA-181a/b-1 enhances chondroprogenitor anabolism and downregulates aquaporin-9","authors":"Austin Bell-Hensley ,&nbsp;Victor Gustavo Balera Brito ,&nbsp;Lei Cai ,&nbsp;Jin Liu ,&nbsp;Kathryn Feeney ,&nbsp;Hongjun Zheng ,&nbsp;Audrey McAlinden","doi":"10.1016/j.ocarto.2024.100550","DOIUrl":"10.1016/j.ocarto.2024.100550","url":null,"abstract":"<div><h3>Objective</h3><div>Effective osteoarthritis treatments that enhance the anabolic/regenerative capacity of chondrocytes are needed. Studying cartilage development processes may inform us of approaches to control chondrocyte differentiation and anabolism and, ultimately, how to effectively treat OA. MicroRNAs are broad-acting epigenetic regulators known to affect many skeletal processes. Previous reports from our group indicated that miR-181a-1 is upregulated during chondrocyte differentiation. The goal of this study was to determine how the entire miR-181a/b-1 cluster regulates <em>in vitro</em> chondrogenesis.</div></div><div><h3>Design</h3><div>Precursor miR-181a/b-1 was over-expressed in cartilage progenitor cells using lentiviral technology Transduced cartilage progenitor cells were cultured as micromass pellets in hypoxic conditions and stimulated to undergo chondrogenic differentiation for five weeks. Bulk RNA-sequencing and immunostaining was applied to evaluate chondrogenic differentiation and matrix production.</div></div><div><h3>Results</h3><div>Immunostaining of cartilage pellet sections showed that miR-181a/b-1 increased mature type II collagen and decreased expression of the chondroprogenitor type IIA collagen isoform. Bulk RNA-Seq at day 7 of chondrogenesis revealed upregulation of pro-anabolic genes such as <em>COL2A1</em>, <em>COL9A2/3</em>, <em>COL11A2</em> and <em>SNORC</em>. Of the genes significantly downregulated by miR-181a/b-1, aquaporin 9 (<em>AQP9</em>) was the top hit which decreased in expression by over 14-fold. While a predicted target of miR-181a/b, our data showed that this miRNA cluster likely suppresses <em>AQP9</em> via an indirect targeting mechanism.</div></div><div><h3>Conclusions</h3><div>Our findings demonstrate a pro-differentiation/anabolic function for miR-181a/b-1 during <em>in vitro</em> chondrogenesis that may be due, in part, to suppression of <em>AQP9</em>. Future studies are needed to elucidate the role of this membrane channel protein in regulating chondrocyte differentiation and homeostasis.</div></div>","PeriodicalId":74377,"journal":{"name":"Osteoarthritis and cartilage open","volume":"7 1","pages":"Article 100550"},"PeriodicalIF":0.0,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142743983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Safety and preliminary efficacy of transcutaneous auricular vagus nerve stimulation on chronic knee pain: A pilot trial","authors":"Kosaku Aoyagi ,&nbsp;Elias Rivas ,&nbsp;Roxanna Shababi ,&nbsp;Robert Edwards ,&nbsp;Michael LaValley ,&nbsp;Julia Lechuga ,&nbsp;Vitaly Napadow ,&nbsp;Tuhina Neogi","doi":"10.1016/j.ocarto.2024.100545","DOIUrl":"10.1016/j.ocarto.2024.100545","url":null,"abstract":"<div><h3>Objective</h3><div>Transcutaneous auricular vagus nerve stimulation (<strong>tVNS</strong>) may be an innovative treatment for symptoms of knee osteoarthritis (OA) due to possible shared pathological mechanisms between diminished parasympathetic function, central pain mechanisms, and knee pain. Thus, we sought to test the safety and preliminary efficacy of tVNS in people with knee OA.</div></div><div><h3>Design</h3><div>A pilot trial in which participants received a 60-min tVNS was conducted. At baseline, immediately after, and 15 ​min after tVNS, we assessed knee pain, pressure pain threshold (<strong>PPT</strong>), temporal summation (<strong>TS</strong>), conditioned pain modulation (<strong>CPM</strong>), and high-frequency power of heart rate variability (<strong>HF</strong>). We examined the extent to which these outcome measures changed after tVNS using linear mixed models.</div></div><div><h3>Results</h3><div>30 participants with knee OA were included, and all completed the intervention without any major side effects. Compared to baseline, knee pain was reduced by 1.27 (95 ​% CI, −1.74, −0.80) immediately after and by 1.87 (−2.33, −1.40) 15 ​min after tVNS; CPM improved by 0.11 (0.04, 0.19) and 0.07 (−0.01, 0.15); and HF improved by 213.29 (−0.38, 426.96) and 234.17 (20.49, 447.84). PPT and TS were not changed after tVNS.</div></div><div><h3>Conclusions</h3><div>Our preliminary data demonstrated that tVNS may be a safe pain-relieving treatment for people with knee OA. Our findings suggest that improvement of knee pain might be derived from improvement of parasympathetic function and central pain mechanisms as no local therapy was applied. A large study is needed to confirm that tVNS is a novel intervention to ameliorate knee pain in people with knee OA.</div></div><div><h3>Clinical Trial</h3><div><span><span>ClinicalTrials.gov</span><svg><path></path></svg></span> (NCT05625178).</div></div>","PeriodicalId":74377,"journal":{"name":"Osteoarthritis and cartilage open","volume":"7 1","pages":"Article 100545"},"PeriodicalIF":0.0,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142743873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study protocol for the PICASSO trial: A randomized placebo-controlled trial to investigate the efficacy and safety of intraarticular steroid injections and an occupational therapy intervention in painful inflammatory carpometacarpal-1 osteoarthritis","authors":"Marthe Gløersen ,&nbsp;Ingvild Kjeken ,&nbsp;A.T. Tveter ,&nbsp;Amirhossein Kazemi ,&nbsp;Joseph Sexton ,&nbsp;Krysia Dziedzic ,&nbsp;David T. Felson ,&nbsp;Tanja A. Stamm ,&nbsp;Ali Guermazi ,&nbsp;Merete Hermann-Eriksen ,&nbsp;M.I. Sæther ,&nbsp;Kristine Lundby ,&nbsp;E.L. Esperø ,&nbsp;Monika Olsen ,&nbsp;K.B. Norheim ,&nbsp;Edle Berg Fister ,&nbsp;Mari Hoff ,&nbsp;Jorunn Kvalø Uleberg ,&nbsp;Irina Petrovna Midtgard ,&nbsp;Therese Andreassen ,&nbsp;Trine Amalie Sjøvold","doi":"10.1016/j.ocarto.2024.100542","DOIUrl":"10.1016/j.ocarto.2024.100542","url":null,"abstract":"<div><h3>Objective</h3><div>Our primary objectives are to assess whether intraarticular corticosteroid injections are superior to saline injections with regards to thumb base pain after 4 weeks, and to compare the efficacy of steroid injections, saline injections, and an occupational therapy intervention on thumb base pain after 12 weeks in people with painful inflammatory osteoarthritis (OA) of the first carpometacarpal (CMC-1) joint.</div></div><div><h3>Design</h3><div>In this three-armed, double-blind, randomized multicenter trial, 354 participants with painful inflammatory CMC-1 OA from six Norwegian hospitals are recruited. Participants are randomized 1:1:1 to intraarticular steroid or saline injections in the CMC-1 joint or a multimodal occupational therapy intervention. The primary outcomes are thumb base pain measured on a numeric rating scale (NRS, range: 0–10) after 4 weeks and 12 weeks. Key secondary outcomes include synovitis by Magnetic Resonance Imaging (MRI) after 4 weeks and hand function by the Measure of Activity Performance of the Hand (MAP-Hand) questionnaire after 12 and 24 weeks. Other secondary outcomes are synovitis by clinical examination and ultrasound, measures of pain, function, stiffness, and health-related quality of life, and direct and indirect costs. Adverse events are recorded at each visit. The duration of the randomized controlled trial is 24 weeks, followed by an 80-week open-label observational phase to investigate the long-term efficacy and safety of repeated steroid injections and the occupational therapy intervention.</div></div><div><h3>Conclusions</h3><div>The results from this trial will have important clinical implications and influence future guidelines on OA management of the CMC-1 joint.</div></div><div><h3>Clinical trial registration</h3><div>EU-CT 2023-505254-17-00, NCT06084364.</div></div>","PeriodicalId":74377,"journal":{"name":"Osteoarthritis and cartilage open","volume":"7 1","pages":"Article 100542"},"PeriodicalIF":0.0,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142721827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomic analysis reveals biomarkers associated with performance-based joint function and patient-reported outcomes in knee osteoarthritis","authors":"Josefine E. Naili ,&nbsp;Aisha S. Ahmed ,&nbsp;Margareta Hedström ,&nbsp;Morten Bilde Simonsen ,&nbsp;Eva W. Broström ,&nbsp;Helena Erlandsson Harris ,&nbsp;Ákos Végvári ,&nbsp;Cecilia Aulin","doi":"10.1016/j.ocarto.2024.100543","DOIUrl":"10.1016/j.ocarto.2024.100543","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to identify proteins associated with clinical manifestations of knee osteoarthritis (KOA), including performance-based joint function and patient-reported outcome measures (PROM).</div></div><div><h3>Methods</h3><div>This cross-sectional exploratory study included thirteen individuals with KOA and eleven age-matched controls. All participants performed the 30s Single Leg Mini Squat test and 30s Sit-to-Stand test with simultaneous recording of joint kinematics. Individuals with KOA completed the Knee Injury and Osteoarthritis Outcome Score and Forgotten Joint Score-12. Proteins were determined by quantitative mass spectrometry (MS) in plasma. Principal component analysis (PCA), hierarchical cluster analysis (HCA), and Reactome enrichment analysis of the proteome were conducted to identify activated pathways and groups.</div></div><div><h3>Results</h3><div>Performance-based function was worse in individuals with KOA compared to controls, and they reported higher levels of pain. MS analysis identified 82 differentially expressed proteins (DEPs) in KOA (28 upregulated, 54 downregulated of 321 detected proteins). PCA displayed distinct features between KOA and controls, similar to HCA, which distinguished two major clusters. Enrichment analysis displayed platelet activation and degranulation, neutrophil, and extracellular matrix (ECM)-related pathways. From the proteome, 23 DEPs were associated with different aspects of joint function, and 25 DEPs with PROM.</div></div><div><h3>Conclusions</h3><div>Individuals with KOA differed from controls across all three assessment modalities; they presented worse joint function, higher levels of pain, and an altered plasma protein profile. Multiple associations were observed between up- and downregulated DEPs and clinical manifestations. The described study protocol shows promise for performing multivariate analyses for future subgrouping of individuals with KOA.</div></div>","PeriodicalId":74377,"journal":{"name":"Osteoarthritis and cartilage open","volume":"7 1","pages":"Article 100543"},"PeriodicalIF":0.0,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142697425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A scoping review of statistical methods to investigate colocalization between genetic associations and microRNA expression in osteoarthritis","authors":"Kathleen Zang ,&nbsp;Myriam Brossard ,&nbsp;Thomas Wilson ,&nbsp;Shabana Amanda Ali ,&nbsp;Osvaldo Espin-Garcia","doi":"10.1016/j.ocarto.2024.100540","DOIUrl":"10.1016/j.ocarto.2024.100540","url":null,"abstract":"<div><h3>Background</h3><div>Genetic colocalization analysis is a statistical method that evaluates whether two traits (e.g., osteoarthritis [OA] risk and microRNA [miRNA] expression levels) share the same or distinct genetic association signals in a locus typically identified in genome-wide association studies (GWAS). This method is useful for providing insights into the biological relevance of genetic association signals, particularly in intergenic regions, which can help to elucidate disease mechanisms in OA and other complex traits.</div></div><div><h3>Objectives</h3><div>To review the existing literature on genetic colocalization methods, assess their suitability for studying OA, and investigate their capacity to integrate miRNA data, while bearing in view their statistical assumptions.</div></div><div><h3>Design</h3><div>We followed scoping review methodology and used Covidence software for data management. Search terms for colocalization, GWAS, and genetic or statistical models were used in the databases MEDLINE and EMBASE, searched till March 4, 2024.</div></div><div><h3>Results</h3><div>Our search returned 546 peer-reviewed papers, of which 96 were included following title/abstract and full-text screening. Based on both cumulative and annual publication counts, the most cited method for colocalization analysis was coloc. Four papers examined OA-related phenotypes, and none examined miRNA. An approach to colocalization analysis using miRNA was postulated based on further hand-searching.</div></div><div><h3>Conclusions</h3><div>Colocalization analysis is a largely unexplored method in OA. Many of the approaches to colocalization analysis identified in this review, including the integration of GWAS and miRNA data, may help to elucidate genetic and epigenetic factors implicated in OA and other complex traits.</div></div>","PeriodicalId":74377,"journal":{"name":"Osteoarthritis and cartilage open","volume":"6 4","pages":"Article 100540"},"PeriodicalIF":0.0,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142697242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risk of all-cause mortality in patients with knee osteoarthritis: A systematic review and meta-analysis of cohort studies","authors":"Pei-En Kao ,&nbsp;Amy Ker","doi":"10.1016/j.ocarto.2024.100541","DOIUrl":"10.1016/j.ocarto.2024.100541","url":null,"abstract":"<div><h3>Objective</h3><div>This systematic review and meta-analysis aimed to evaluate the risk of all-cause mortality in patients with knee osteoarthritis (OA).</div></div><div><h3>Design</h3><div>Comprehensive searches were conducted in PubMed, Embase, and the Cochrane Library on September 01, 2024. The review included cohort studies reporting risk estimates of all-cause mortality in knee OA patients compared to those without knee OA. Using a random-effects model, the pooled hazard ratios (HRs) were calculated. Subgroup analyses were performed according to the classification of knee OA, including radiographic knee OA only, symptomatic knee OA only, and radiographic and symptomatic knee OA.</div></div><div><h3>Results</h3><div>A total of 15 cohort studies involving 1,023,799 participants were included in the systematic review, with 14 studies remaining for the meta-analysis. The meta-analysis revealed that knee OA patients had an increased risk of all-cause mortality compared to those without knee OA (pooled HR: 1.21; 95% confidence interval [CI]: 1.02, 1.45). Subgroup analyses indicated the mixed results, including radiographic knee OA only (pooled HR: 1.11; 95% CI: 0.97, 1.26), symptomatic knee OA only (pooled HR: 1.07; 95% CI: 0.80, 1.43), and radiographic and symptomatic knee OA (pooled HR: 1.58; 95% CI: 1.20, 2.07).</div></div><div><h3>Conclusions</h3><div>This meta-analysis supports an association between knee OA and an increased risk of all-cause mortality, with a particularly pronounced risk observed in radiographic and symptomatic knee OA patients. Further research is needed to determine if OA at other sites also correlates with a higher risk of all-cause mortality.</div></div>","PeriodicalId":74377,"journal":{"name":"Osteoarthritis and cartilage open","volume":"7 1","pages":"Article 100541"},"PeriodicalIF":0.0,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142697418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peripheral arterial pathology and osteoarthritis of the knee: US examination of arterial wall stiffness, thickness, and flow characteristics","authors":"Jon Olansen ,&nbsp;Minglang Yin ,&nbsp;Janine Molino ,&nbsp;Thomas Carruthers ,&nbsp;Derek Jenkins ,&nbsp;George Karniadakis ,&nbsp;Roy K. Aaron","doi":"10.1016/j.ocarto.2024.100537","DOIUrl":"10.1016/j.ocarto.2024.100537","url":null,"abstract":"<div><h3>Background</h3><div>Osteoarthritis (OA) is a widespread chronic joint disorder characterized by the degeneration of articular cartilage, extensive bone remodeling, ligamentous fibrosis, and synovial inflammation impacting millions. Shared factors like phenotypic similarities, hypofibrinolysis, and inflammation constitute similarities in pathophysiology and clinical manifestations between OA and peripheral vascular disease (PVD). This study investigated peripheral arterial flow characteristics, vascular wall thickness, and stiffness in knee OA to clarify a potential association with early atherosclerosis.</div></div><div><h3>Methods</h3><div>The OA cohort consisted of 35 knees with a mean age of 69 years. The control cohort consisted of 58 knees with a mean age of 68 years. Subjects underwent arterial ultrasound scanning of the common femoral, superficial femoral, and popliteal arteries. Data measured included peak systolic volumetric flow, intima-media thickness, systolic and diastolic vessel diameter, and simultaneous EKG and flow curves. Structural and functional vascular data were quantified using the incremental Young's modulus, pulse wave velocity (PWV), and distensibility.</div></div><div><h3>Results</h3><div>Significantly elevated arterial volumetric flow, measures of arterial stiffness, and intima-media wall thickness were observed in those with OA compared to those without. PWV as calculated by the Bramwell-Hill equation were found to be significantly greater in all three vessels of patients with OA.</div></div><div><h3>Conclusions</h3><div>This study supports the association between peripheral arterial pathology and knee OA, consistent with shared clinical and phenotypic traits. The observed characteristics of early vascular pathology suggest potential pathophysiologic linkages between OA and PVD. This foundational framework provides avenues for mechanistic studies exploring the relationship between these two disease processes.</div></div>","PeriodicalId":74377,"journal":{"name":"Osteoarthritis and cartilage open","volume":"6 4","pages":"Article 100537"},"PeriodicalIF":0.0,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142592969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of cartilage T1ρ and T2 relaxation time measurement with hip osteoarthritis progression: A 5-year longitudinal study using voxel-based relaxometry and Z-score normalization","authors":"Rafeek Thahakoya ,&nbsp;Koren E. Roach ,&nbsp;Misung Han ,&nbsp;Rupsa Bhattacharjee ,&nbsp;Fei Jiang ,&nbsp;Johanna Luitjens ,&nbsp;Emma Bahroos ,&nbsp;Valentina Pedoia ,&nbsp;Richard B. Souza ,&nbsp;Sharmila Majumdar","doi":"10.1016/j.ocarto.2024.100538","DOIUrl":"10.1016/j.ocarto.2024.100538","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Objective&lt;/h3&gt;&lt;div&gt;To study the longitudinal changes of cartilage &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msub&gt;&lt;mi&gt;T&lt;/mi&gt;&lt;mrow&gt;&lt;mn&gt;1&lt;/mn&gt;&lt;mi&gt;ρ&lt;/mi&gt;&lt;mspace&gt;&lt;/mspace&gt;&lt;/mrow&gt;&lt;/msub&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; and &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msub&gt;&lt;mi&gt;T&lt;/mi&gt;&lt;mn&gt;2&lt;/mn&gt;&lt;/msub&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; relaxation time measurements in hip-OA patients.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Methods&lt;/h3&gt;&lt;div&gt;A calibration study compared two scanner data, Scanner-1 (GE Discovery MR750 3.0T) with unilateral acquisition protocol and Scanner-2 (GE Signa Premier 3.0T) with bilateral acquisition protocol, using nine subjects(average age ​= ​40.33 ​± ​13.53 years, 5 females), including one hip-OA subject. Quantified parameters from the Scanner-2 were adjusted using voxel-based relaxometry(VBR) and Z-score normalization to reduce the inter-scanner variability. Eighteen hip-OA Subjects (age ​= ​53.11 ​± ​14.96 years, 12 females) were recruited to the longitudinal variability study from 2016, comprising five assessments at 1-year intervals. Baseline to 3rd-year data used unilateral acquisition with Scanner-1, while 4th-year data used bilateral acquisition with Scanner-2. A linear mixed-effects model(LME) assessed trajectory analyses, with acquisition year, age, sex, body mass index(BMI), and Kellgren-Lawrence(KL) score as predictor variables and cartilage mean &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msub&gt;&lt;mi&gt;T&lt;/mi&gt;&lt;mrow&gt;&lt;mn&gt;1&lt;/mn&gt;&lt;mi&gt;ρ&lt;/mi&gt;&lt;mspace&gt;&lt;/mspace&gt;&lt;/mrow&gt;&lt;/msub&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; and &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msub&gt;&lt;mi&gt;T&lt;/mi&gt;&lt;mn&gt;2&lt;/mn&gt;&lt;/msub&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; values as outcomes.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;VBR analysis after Z-score normalization showed that only a few of the whole cartilage voxels had significant differences in &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msub&gt;&lt;mi&gt;T&lt;/mi&gt;&lt;mrow&gt;&lt;mn&gt;1&lt;/mn&gt;&lt;mi&gt;ρ&lt;/mi&gt;&lt;mspace&gt;&lt;/mspace&gt;&lt;/mrow&gt;&lt;/msub&gt;&lt;mo&gt;(&lt;/mo&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt;femur-2.36 ​% and acetabular-3.23 ​%) and &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msub&gt;&lt;mi&gt;T&lt;/mi&gt;&lt;mn&gt;2&lt;/mn&gt;&lt;/msub&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; (femur-2.30 ​% and acetabular-2.94 ​%) values between the scanners. The LME analysis showed that the BMI predictor variable was significantly correlated with the femur &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msub&gt;&lt;mi&gt;T&lt;/mi&gt;&lt;mrow&gt;&lt;mn&gt;1&lt;/mn&gt;&lt;mi&gt;ρ&lt;/mi&gt;&lt;mspace&gt;&lt;/mspace&gt;&lt;/mrow&gt;&lt;/msub&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; (p ​&lt; ​0.0001) and &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msub&gt;&lt;mi&gt;T&lt;/mi&gt;&lt;mn&gt;2&lt;/mn&gt;&lt;/msub&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; (p ​&lt; ​0.0001) and acetabular &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msub&gt;&lt;mi&gt;T&lt;/mi&gt;&lt;mrow&gt;&lt;mn&gt;1&lt;/mn&gt;&lt;mi&gt;ρ&lt;/mi&gt;&lt;mspace&gt;&lt;/mspace&gt;&lt;/mrow&gt;&lt;/msub&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; (p ​&lt; ​0.0001) and &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msub&gt;&lt;mi&gt;T&lt;/mi&gt;&lt;mn&gt;2&lt;/mn&gt;&lt;/msub&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; (p ​&lt; ​0.0001) cartilage region.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Conclusion&lt;/h3&gt;&lt;div&gt;The calibration study demonstrated the effectiveness of VBR and Z-score normalization in reducing inter-scanner variability. The longitudinal study revealed a significant correlation between &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msub&gt;&lt;mi&gt;T&lt;/mi&gt;&lt;mrow&gt;&lt;mn&gt;1&lt;/mn&gt;&lt;mi&gt;ρ&lt;/mi&gt;&lt;mspace&gt;&lt;/mspace&gt;&lt;/mrow&gt;&lt;/msub&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; and &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msub&gt;&lt;mi&gt;T&lt;/mi&gt;&lt;mn&gt;2&lt;/mn&gt;&lt;/msub&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; value","PeriodicalId":74377,"journal":{"name":"Osteoarthritis and cartilage open","volume":"6 4","pages":"Article 100538"},"PeriodicalIF":0.0,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142571626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bone marrow lesions in osteoarthritis: Characterising genetic and histological changes to understand disease pathophysiology","authors":"Nidhi Sofat ,&nbsp;Franklyn Arron Howe","doi":"10.1016/j.ocarto.2024.100531","DOIUrl":"10.1016/j.ocarto.2024.100531","url":null,"abstract":"<div><div>Osteoarthritis (OA) is a chronic debilitating condition that affects the whole joint. There are several sources of pain in OA that include the synovium, bone, including osteophytes and more recently bone marrow lesions (BML) that correlate with pain. Recent studies have shown that the bone compartment contributes to pain in OA through the development of OA-BMLs which are richly innervated and demonstrate angiogenesis. The synovium is also innervated in OA tissue and is another distinct source of pain, with imaging and genetic studies supporting the observation that synovitis is an important component of pain in OA. Previous studies using magnetic resonance imaging (MRI) have shown that bone marrow lesions (BMLs), observed as high intensity signal on T2 fat-suppressed imaging sequences, are commonly found in OA and are associated with progression of pain symptoms. Recent studies have described the genetic signature of BMLs and the characteristic histological changes of BML tissue. In this narrative review we describe the recent developments in the discovery of the gene expression profiles identified from BMLs. We also review the recently characterised histological changes from BMLs in large weight-bearing joints including the knee and hip. Finally, we discuss the implications of new genetic and histological findings in BML in the context of new developments for pharmacological therapies in OA.</div></div>","PeriodicalId":74377,"journal":{"name":"Osteoarthritis and cartilage open","volume":"6 4","pages":"Article 100531"},"PeriodicalIF":0.0,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142571627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}