American journal of dentistry最新文献

Purpose: To evaluate the laboratory microtensile bond strength (µTBS) of universal adhesives applied in etch-and-rinse mode on irradiated and sound human dentin.

Methods: Dentin specimens of 135 freshly extracted human third molars were divided in three superordinate groups. Each tooth received either no irradiation (control group), 5 Gy (2 Gy/2 Gy/1 Gy in 3 consecutive days) or the high-level therapeutic dose of 60 Gy (test groups) applied fractionally (2 Gy/day, 5 days/week, 6 weeks). Samples from each group were randomly divided into three subgroups assigned to three established universal adhesives (Futurabond U, AdheSE Universal, Xeno Select) in just the etch-and-rinse application mode (n= 15). Bond strength was assessed with a universal testing machine 15 minutes after application and light curing of the adhesive materials. Statistical analysis was performed using a two-way ANOVA (P< 0.01) and Tukey's test (P< 0.05).

Results: Regarding all experimental groups ANOVA showed significant effects on µTBS of dentin irradiation and the used adhesives (P< 0.01, ANOVA). In nearly all groups, a decrease of µTBS was observed when used on highly irradiated dentin. However, comparing the different groups, no significant influence of high-level radiation on the microtensile bond strength of the three universal adhesives assessed in etch-and-rinse application mode could be determined (P> 0.05, Tukey's test).

Clinical significance: Within the limitations of this laboratory study, no significant effects of low- and high-level tumor irradiation on the bond strength of universal adhesives can be detected after application in etch-and-rinse mode. Consequently, this mode of application may be considered a viable option for patients who have undergone radiation therapy when using universal adhesive systems for luting restorative materials.

Purpose: To evaluate the effect of different infection control barriers used with light curing units (LCU) on the shear bond strength (SBS) of glass ceramics with various translucencies and thicknesses during adhesive cementation.

Methods: 60 extracted molar teeth cut in halves mesio-distally to obtain 120 enamel surfaces. Lithium disilicate blocks (GC, Initial Lisi Blocks); 30 High Translucency (HT) 0.5 mm, 30 Low Translucency (LT) 0.5 mm, 30 HT 1.0 mm, 30 LT 1.0 mm discs with 3 mm diameter were produced (n=120). Samples were categorized into three groups according to the infection control barriers (n=40): 1-LCU without barrier, 2-Disposable barrier on LCU (Pinnacle Cure Sleeve), 3-LCU wrapped with stretch film. The SBS test was performed after thermocycling. Failure modes were determined at x20 and classified and statistically analyzed (P< 0.05).

Results: Differences between all the groups were significant (P< 0.001). The highest SBS values were obtained as HT LCU 0.5 mm, LT LCU 0.5 mm, HT Sleeve 0.5 mm and LT Sleeve 0.5 mm, respectively. Highest SBS values were seen in HT LCU 0.5 mm (16.51± 0.68), while LT 1.0 mm stretch (10.76± 1.37) showed the lowest values. Mostly mixed failures occurred in the 0.5 mm sample groups showing higher SBS values.

Clinical significance: While the application of a sleeve on a LCU may slightly reduce the shear bond strength values of glass ceramics, it can still be beneficial for the cementation of these materials as it helps prevent cross-infection.

Purpose: To systematically evaluate the effect of bisphosphonates on periodontal diseases in menopausal and postmenopausal women.

Methods: Databases (PubMed, Embase, Web of Science, Cochrane Library databases, Chinese Scientific and Technological Journal database, Wan Fang Data, China Biomedical Literature Database, and Chinese National Knowledge Infrastructure) were searched from inception to July 2024, languages are Chinese and English. Randomized controlled trials (RCTs) reporting the effect of bisphosphonates in menopausal and postmenopausal women with periodontitis were included. The risk of bias was performed using the Cochrane collaboration tool. The primary outcome was clinical attachment loss (CAL), and the secondary outcomes were probing depth (PD) and gingival index (GI). The analysis of the data was performed using Rev Man 5.3 and Stata 16.0.

Results: The meta-analysis incorporated four studies that fulfilled the inclusion criteria. In evaluating the efficacy of bisphosphonates against control treatments, there was high heterogeneity observed in CAL (P = 0.0002; I² = 85%) and PD (P< 0.00001; I² = 93%) within the study groups. Meta-analysis showed a significant improvement in CAL gain (MD = - 0.57 mm; 95% CI = -1.04 to -0.11 mm; P< 0.05), PD reduction (MD = - 0.50 mm; 95% CI = -0.96 to -0.05 mm; P< 0.05), and GI reduction (MD = -1.11; 95% CI = -1.22 to -1.01; P< 0.00001) for bisphosphonate treatment vs. bisphosphonate-naïve therapy.

Clinical significance: Bisphosphonate treatment seems to be beneficial for managing periodontitis in menopausal and postmenopausal women.

Purpose: To evaluate the effect of toothpaste containing sodium fluoride, a commercially available remineralization product, on the microhardness and roughness of enamel after whitening with two different concentrations of hydrogen peroxide.

Methods: 32 premolar teeth extracted for orthodontic reasons were collected. Initial microhardness and roughness values were measured. All samples were immersed in a coffee solution for 1 week to simulate coloration in the oral environment and ensure standardization. Then, the second microhardness and roughness measurements were obtained. Two samples were separated, and the remaining ones were divided into three groups; Group 1: Group 2: Samples treated with Beka HP 30% bleaching agent; Group 3: Samples kept in distilled water. For Groups 1 and 2, the bleaching procedure was applied in three sessions of 15 minutes each, following the manufacturer's instructions. Afterward, measurements of all samples were repeated. To examine the reversibility of surface changes following the bleaching process, a demineralization-remineralization (pH) cycle was applied. Subsequently, microhardness and roughness measurements were repeated, and two samples from each group were set aside for FE-SEM Field emission scanning electron microscope analysis. A total of eight samples underwent FE-SEM analysis at x1,000 and x5,000 magnifications. Data were analyzed using two-way ANOVA and Kruskal-Wallis tests.

Results: Initial microhardness values of all samples were the highest values, with a decrease observed after the bleaching procedure. Even though the microhardness of the samples increased in Groups 1 and 2 after the pH cycle, there was no significant difference (P< 0.05). While the roughness values of the samples decreased after the discoloration (P> 0.05), they increased after the pH cycle and there were significant differences between the 4th measurement and the other measurements (P< 0.05).

Clinical significance: The bleaching agent causes changes in the structure of the enamel and reduces microhardness and roughness values. However, remineralizing agents applied during the pH cycle may have a positive effect on microhardness.

Purpose: To assess if amino acid-based carbon dots (CDs) eliminate in vitro cancer cells.

Methods: CDs were synthesized using cysteine as a precursor and metal and photosensitizer as modifiers. In vitro cell viability tests were performed using cancer and normal cells without and with H₂O₂ and laser irradiation. Methylene blue (MB) solution was used to evaluate the production of ROS by CDs via observing absorbance differences before and after treatments. Extracellular oxygen production was measured in real-time using a probe. The generation of singlet oxygen was spectroscopically evaluated using RNO-ID assay.

Results: Within the test conditions, CDs fn-Cei-Ce6 achieved significantly low in vitro cancer cell viability (near to 26% of initial cell count) at 20 ppm concentration, high absorbance difference (Δ) in all wavelengths, oxygen production approximately to 1.2 mg/L, and high absorbance decrease of RNO-ID assay solution all with 100 µM H₂O₂ and laser irradiation conditions.

Clinical significance: Amino acid-based carbon dots (CDs) eliminated cancer cells in vitro with near to 74% of initial cancer cells under 100 µM H₂O₂ and laser irradiation conditions. The results may be extended to the clinical potential of fn-Cei-Ce6 in treating tumors in the oral cavity.

Purpose: To investigate the shear bond strength of different primers with adhesive cements to 3D printed restorations.

Methods: 72 3D-printed restorations (Formlabs permanent crown resin) and nano-hybrid composite (Tetric EvoCeram) resin cylinders prepared and assigned to two groups, according to G-Multi Primer (GMP) with G-Cem One (GCO) and Clearfil ceramic primer plus (CCP) with Panavia F2.0 (PF) cements and three subgroups (n=9) were created for each cement: (1) control group, (2) 5,000 thermal cycles, (3) 10,000 thermal cycles. 3D-printed restorations were embedded in Teflon molds and cemented with nano-hybrid composite resin cylinders and a shear bond strength test was performed according to group. Failure modes were observed with stereomicroscope (30x), and surface morphology was evaluated with SEM analysis (100x and 2,000x). Statistical analysis was performed using Kolmogorov-Smirnov and Shapiro Wilks tests, and for evaluating the study data, 2-way ANOVA test and post hoc Tukey HSD test were used.

Results: The GCO groups exhibited significantly higher SBS values than the PF groups in the bonding of the tested 3D-printed restorations. There was no statistically significant difference in the bond strengths of tested GCO cement groups. A control group of PF cement showed significantly higher SBS values than the 5,000 and 10,000 cycling of PF groups. However, there was no statistically significant difference in the 5,000 and 10,000 cycling PF groups. The bonding performance was superior for the GCO group.

Clinical significance: This study showed that exposure to gutta-percha solvents (chloroform, orange oil and Endosolv) for 5 minutes did not affect the bond strengths of both iRoot SP (bioceramic sealer) and AH Plus (resin sealer) to root dentin.

Purpose: To assess the relative efficacy of a new entry-tier oscillating-rotating (OR) electric toothbrush versus a sonic electric toothbrush over 4 weeks of use for plaque and gingivitis reduction.

Methods: This single-center, examiner-blind, two-treatment, parallel-group, randomized clinical study enrolled adult participants who had evidence of gingivitis and plaque at baseline. Participants were randomly assigned to use either an entry-tier OR toothbrush (Oral-B iO2) with the Ultimate Clean brush head in Daily Clean mode or an advanced sonic toothbrush (usmile Marble-Art) used with the usmile Advanced Whitening brush head in Clean mode (and at the high-intensity level). Both groups brushed with a standard sodium fluoride dentifrice. Participants were assessed for gingivitis (Modified Gingival Index and the Gingival Bleeding Index) and plaque (Rustogi Modification of the Navy Plaque Index) at baseline and after 4 weeks of twice-daily use. Plaque was also assessed after a single use at baseline.

Results: Both toothbrushes statistically significantly reduced gingivitis after 4 weeks of use and plaque after a single use and after 4 weeks (P< 0.001 for all). The OR toothbrush, relative to the sonic toothbrush, demonstrated a statistically significantly greater reduction in Modified Gingival Index score and number of bleeding sites (P< 0.001) and was associated with a significantly greater number of users transitioning from gingivitis to a state of gingival health (i.e., < 10% bleeding sites; P= 0.038) by the end of the study. The OR toothbrush likewise demonstrated greater efficacy with respect to plaque reduction after a single use (whole mouth, interproximal, and gingival margin; P< 0.001 for all) and after 4 weeks of use (whole mouth and interproximal; P< 0.001 for both).

Clinical significance: The novel entry-tier OR toothbrush offers significantly greater plaque control and gingivitis reduction relative to the advanced sonic toothbrush model.

Purpose: To evaluate the surface roughness (SR) and surface topography (ST) of mechanical brushing with pure-unfilled PEEK (U-PEEK) and PEEK materials with different contents [titanium-containing PEEK (T-PEEK), ceramic-modified PEEK (C-PEEK) and glass fiber-reinforced PEEK (G-PEEK)].

Methods: A total of 40 disc-shaped samples (10 mm x 2 mm) were obtained (n= 10) from each group (U-PEEK, T-PEEK, C-PEEK, G-PEEK). After sanding and polishing, a profilometer was used to analyze all discs' surface roughness (Ra). Qualitative analysis (QA) was performed on random samples with the help of a scanning electron microscope (SEM). Brushing was done with a custom-made brushing simulator, and the analyses were repeated. Statistical analysis was performed (P< 0.05).

Results: A statistically significant difference was found between the PEEK groups before and after brushing (P< 0.05). In the measurements before and after brushing, the lowest measurement value was seen in the C-PEEK and T-PEEK groups. While there was no significant difference between T-PEEK and C-PEEK measurements, both measurements found the highest measurement value in the G-PEEK group. In addition, in all groups, the average roughness values after brushing were statistically significantly higher than those before brushing. All groups except G-PEEK are below the clinically acceptable SR value (0.2 µm) before and after brushing. T-PEEK and C-PEEK show better SR values.

Clinical significance: With this study, knowing the effect of mechanical brushing on the SR of modified PEEK materials which may become widely used in dentistry, will be a guide for clinicians.

Purpose: To determine the effect of charcoal-based toothpaste used alone or in combination with charcoal-based mouthwash on color change (ΔE₀ ₀) and surface roughness (Ra) of a stained resin composite.

Methods: Disc-shaped (8 x 2 mm) resin composite samples (Filtek Z250) were prepared. Then, the samples were immersed in coffee for 12 days. The samples were randomly assigned into four groups (n= 12). Distilled water (DW), Colgate Total 12 (CT), Colgate Optic White (COW), and Colgate Optic White + Colgate Plax White Charcoal (COW + CPWC) were used over a 3-month period. Color measurements were performed at baseline, after staining, after 1 and 3 months use. Ra analysis and surface topography by scanning electron microscopy (SEM) were evaluated after staining, after 1 and 3 months use.

Results: Color changes occurring on resin composite samples immersed in coffee for 12 days exceeded the 50:50% acceptability threshold (AT) (ΔE₀ ₀:1.8). CT, COW, and COW + CPWC reduced the color change on composite samples below to the AT after 3 months of use. CT and COW + CPWC showed a significant increase in Ra after both time intervals and caused changes in surface morphology of resin composite. The present findings suggest that charcoal-based toothpaste used alone may be effective in improving the color of resin composite without inducing surface damage. Moreover, when used with charcoal-based mouthwash, the similar effect in color improvement may be accompanied by increased surface roughness.

Clinical significance: This is the first study evaluating the effect of charcoal-based mouthwash when used with charcoal-based toothpaste on color change and surface roughness of a stained resin composite. Both toothpastes tested were effective in improving the color of the resin composite samples. While conventional toothpaste caused increased surface roughness, the charcoal-based toothpaste maintained surface integrity. However, charcoal-based toothpaste did not provide further color improvement when used in combination with the charcoal-based mouthwash and specifically led to increased surface roughness, highlighting potential implications for clinical practice.

Purpose: To investigate the relationship between type 1 diabetes mellitus (T1-DM) and apical periodontitis (AP). The periapical and endodontic conditions of T1-DM individuals were compared with healthy people.

Methods: T1-DM subjects aged 18-45 with good glycemic control (HbA1c < 7) were included in this research. Individuals without systemic disease, twice as many as diabetic patients, were selected as the control group by matching them in terms of age and gender. The subjects' periapical and endodontic statuses were determined by assessing their existing digital panoramic radiographs.

Results: This study included 226 subjects with T1-DM, 101 males and 125 females (30.78 ± 8.59 years). For the control group, 452 subjects without systemic disease, 224 males and 228 females (30.26 ± 8.64 years) were selected. The mean number of teeth in the control group was significantly higher than in the T1-DM group (P = 0.001). However, no significant difference was found between the T1-DM and control groups in terms of endodontic and periapical conditions (P > 0.05). Bivariate logistic regression analysis revealed a positive association between T1-DM and the number of teeth with AP only (P = 0.001; OR = 1.476). There was no association between T1-DM and endodontic conditions or other periapical health-related variables.

Clinical significance: T1-DM patients lost more teeth than the control individuals. As the number of teeth with AP increased, individuals were more likely to be in the T1-DM group. Healthcare professionals should inform patients with Type 1 diabetes about oral health for a better quality of life.