Bilten za hematologiju i transfuziju最新文献

In persons with chronic active hepatitis (35) and chronic persistent hepatitis (23) the CH 50 values, Clq, Cls, C4, C3, C5, and C9 as well as Cl Inh and C3A levels have been determined. The concentration changes of complement (C) were not conditioned by the existence in serum HBs. The degree of changes in values of C in all patients is in determinate relation with the levels of bilirubin, transaminases and immunoglobulins (G, M, A). The degree of determinate changes of C depends on the severity of clinical picture. The degree of Cl Inh elevation is in correlation with intensity of complement activation. The noticed dissociated values of subcomplements Clq and Cls suggest the presence of immune complexes which could influence the HBsAg detection.

Effects of long term plasmapheresis on blood constituents was examined in the plasma donors from whom 220-660 ml of plasma was withdrawn weakly. The donors were submitted 3-300 times to double plasmapheresis procedures. Small decreases in concentrations of total proteins, albumins, gamma-globulins, immunoglobulins IgA and IgM were observed as well as the increases in the concentrations of alpha-1-globulins (p less than 0,05), alpha-2-globulins (p less than 0,05), beta-globulins (p less than 0,05) and immunoglobulins IgG (p less than 0,05). The statistically significant changes were observed earlier in the course of plasmapheresis in donors submitted to more intensive regime.

In human red blood cell there is complete adenyl cyclase--cAMP system. However, the role of this system and its connection with energy metabolism in red cell is not known. Hence, we studied the effects of cAMP and DB-cAMP on energy metabolism in human red cell. Blood was taken out from haematologically healthy volunteers. Red cells were isolated by differential centrifugation and washed three times in Tris-Ringer buffer (pH 7.4). The red cells suspensions (in the above buffer) were simultaneously incubated without additions (control) and with cAMP or DB-cAMP (5 mmol/L). Incubation temperature was 37 degrees C. The samples of suspensions for the extraction of metabolites and cofactors were taken at 0 and 180 minutes of incubation. The lactate, glucose, G6P, F6P, TP, ATP, ADP and AMP were determined in neutralized perchloric acid extracts by specific enzymatic methods. Results of our experiments show that cAMP and DB-cAMP significantly increase red cell glycolysis. Under the same conditions these nucleotides induce positive "cros-over" point at the phosphofructokinase (PFK) step of the glycolysis. The levels of adenine nucleotides (ATP, ADP, AMP) were unchanged through a whole period of incubation. We concluded that cAMP and DB-cAMP stimulates glycolytic process in red cells probably by allosteric activation of PFK.

The frequency of alpha thalassemia in SR Macedonia was determined with studies of Hb Bart's in 1.140 newborn babies. Hb Bart's was found in 83 infants. Distribution of the levels of Hb Bart's in these neonates, as determined by column chromatography on CM Sephadex, was trimodal. The mean values for Hb Bart's in the three groups were 0.5% (SD = 0.22), 1.61% (SD = 0.48), and 4.88% (SD = 0.81). The first group is believed to result from asynchronism of the neonatal "switch off" of gamma chains and activation of beta chain production. The second and the third group represent alpha thal2 and alpha thal1, respectively. Thus, the incidence of beta thal2 in SR Macedonia is 2.4%, and that of alpha thal1 0.8%. Hb H disease was found in three out of 16.000 school children. This form of alpha thalassemia was also found in five out of 2.800 patients examined for the course of anemia. Biosynthetic studies of family members of the eight individuals with Hb H disease showed that one parent is heterozygous alpha thal1 while the other is heterozygous alpha thal2. All individuals with alpha thalassemia had a reduced rate of synthesis of the alpha chains. The mean alpha/beta total activity ratio in individuals with Hb H disease was 0.51 (+/- 0.08), in heterozygous alpha thal1 0.74 (+/- 0.06), and in heterozygous alpha thal2 0.86 (+/- 0.06). Experiments with in vitro translation of globin mRNK isolated from patients with Hb H disease showed lower alpha/beta ratios (0.06) than the intact cell ratios. These results support previously published data that in Hb H disease and alpha thalassemia trait, there is quantitative deficit in alpha globin mRNK, which is a consequence of deleted alpha globin genes.

The paper presents a patient J.S., aged 2 1/2 years, with thrombocytopenia, presence of giant thrombocytes and basophile inclusions in the granulocytes. The same lesions were found in the child's father, followed by increased tendency to bleeding also confirmed in other four family members. Familiar character of the disease and morphologic anomaly of thrombocytes and leucocytes were the basis in passing the diagnosis of May-Hegglin anomaly. The specific features of the case are the child's age and the appearance of hemorrhagic syndrome in infancy.

Non-agglutinating character of some anti-rabbit erythrocytes (RaRBC) antibodies was demonstrated. Non-agglutinating ("incomplete") antibodies were identified mainly as IgG but in some sera were detected also in IgA and IgD class using indirect Coombs test with monospecific antisera. Neutralization and separation of "complete" (IgM) antibodies were performed using 2-mercaptoethanol treatment and/or preparative immunochemical techniques. Some kind of restriction to kappa light chains and to IgG2 subclass has also been observed but monoclonal character of "incomplete" antibody was excluded. Specificity of reaction of IgG anti-RaRBC antibodies with RaRBC membrane antigens was documented using multiple adsorption-identification experiments. Non-specific (Fc-mediated) reaction was excluded in contrast to previously published results on the presence of Fc receptor on RaRBC.

The authors convey their experience gained during the work with Haemonetics M-30 Separator. They had performed 80 separations in total. They had separated leukocytes and thrombocytes simultaneously for 67 times. Most of the donors (51) had been stimulated by corticosteroids in order to obtain the increase of yield. The average concentrate quantity was 355 +/- 91 ml. The leukocytes yield in the concentrate was means = 27,3 +/- 7,8 x 10(9)/1, with 19,2 +/- 5,6 x 10(9)/1 (70%) of polymorphonuclears. The thrombocytes yield was means = 7,80 +/- +/- 1,83 x 10(11)/1. Only thrombocytes were separated 13 times. The average concentrate quantity was 273 +/- 77 ml. The thrombocytes yield in the concentrate was 8,94 +/- 1,26 x 10(11)/1. During the study, the symptoms and signs of hypotony appeared in one the donors.