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Clinical Implications of Interleukin-4 and C-reactive Protein in Atopic Dermatitis and Their Changes Before and after Dupilumab Treatment. 特应性皮炎患者白细胞介素-4和C反应蛋白的临床意义及其在杜匹单抗治疗前后的变化
IF 1.9 4区 医学 Q3 INTEGRATIVE & COMPLEMENTARY MEDICINE Pub Date : 2025-01-01
Tian Zhao, Yinglei Wu, Zhina Shi, Jing Zhang, Chunjie Geng, Yanan Jia, Yaohui Yuan

Objective: To analyze the clinical implications of C-reactive protein (C-reactive protein) and interleukin-4 (IL-4) in atopic dermatitis and their correlations with the therapeutic effect of Dupilumab (DU).

Methods: Seventy-four cases of atopic dermatitis (intervention group) were admitted to Xingtai Third Hospital between May 2021 and January 2023, and 55 concurrent healthy controls (control group) were selected as research participants. Atopic dermatitis patients were treated with a DU injection of 600 mg for the first time after diagnosis. Peripheral blood IL-4 and C-reactive protein levels before and after treatment in the intervention group and their levels at admission in the control group were comparatively analyzed, and their predictive value for the occurrence, clinical efficacy, and adverse reactions of atopic dermatitis were determined. Additionally, alterations in C-reactive protein and IL-4 levels before and after treatment in the intervention group and their relationship with the Scoring Atopic Dermatitis (SCORAD) index were discussed.

Results: The intervention group exhibited higher C-reactive protein and IL-4 levels than the control group. The diagnostic sensitivity and specificity of C-reactive protein + IL-4 detection for atopic dermatitis were 74.32% and 94.55%, respectively (P < .05). The post-treatment C-reactive protein and IL-4 were lower in the intervention group, and the test results were positively correlated with SCORAD before and after treatment (P < .05). In addition, C-reactive protein + IL-4 detection showed excellent predictive effects on the therapeutic efficacy of DU and adverse reactions.

Conclusions: IL-4 and C-reactive protein are closely related to atopic dermatitis, which can be used as the evaluation indexes for disease development of atopic dermatitis and therapeutic effects of DU in the future.

目的分析特应性皮炎患者C反应蛋白(C-reactive protein)和白细胞介素-4(IL-4)的临床意义及其与杜比单抗(Dupilumab,DU)疗效的相关性:方法:选取2021年5月至2023年1月期间邢台市第三医院收治的74例特应性皮炎患者(干预组)和55例同期健康对照者(对照组)作为研究对象。特应性皮炎患者在确诊后首次接受600毫克DU注射治疗。对干预组治疗前后的外周血 IL-4 和 C 反应蛋白水平与对照组入院时的水平进行比较分析,并确定其对特应性皮炎的发生、临床疗效和不良反应的预测价值。此外,还讨论了干预组治疗前后 C 反应蛋白和 IL-4 水平的变化及其与特应性皮炎评分(SCORAD)指数的关系:结果:干预组的 C 反应蛋白和 IL-4 水平高于对照组。C反应蛋白+IL-4对特应性皮炎的诊断敏感性和特异性分别为74.32%和94.55%(P < .05)。干预组治疗后的 C 反应蛋白和 IL-4 均较低,且检测结果与治疗前后的 SCORAD 呈正相关(P < .05)。此外,C反应蛋白+IL-4检测对DU的疗效和不良反应有很好的预测作用:结论:IL-4和C反应蛋白与特应性皮炎密切相关,今后可作为特应性皮炎病情发展和DU疗效的评价指标。
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引用次数: 0
Improvement of Pre-check and Triage Efficiency and Guarantee of Maternal and Infant Safety in Pandemic Situation. 提高预检和分诊效率,保障大流行情况下的母婴安全。
IF 1.9 4区 医学 Q3 INTEGRATIVE & COMPLEMENTARY MEDICINE Pub Date : 2025-01-01
Zhibin Lin, Yanhua Ding, Xiaohui Gan, Juling Wang, Haidong Zheng, Wenjun Li, Zhichuan Chen

Objective: The objective of this study was to enhance the efficiency of pre-check and triage procedures in gynecology and obstetrics, while ensuring the safety of maternal and infant patients during the COVID-19 pandemic.

Methods: Between March 2020 and July 2022, the workflow in gynecology and obstetrics was optimized, and the management of medical staff working in outpatient, ward, and obstetric ward settings was strengthened. Special protocols were developed and implemented for pregnant women, parturients, and neonates. Detailed procedures and routes were established for patient movement from outpatient areas to wards, with strict adherence to pandemic prevention and control measures. Information-based methods were employed to track and monitor the health status of high-risk pregnant women, parturients, and their families, facilitating accurate and efficient pre-check and triage processes.

Results: The implementation of these measures yielded favorable outcomes. No cases of COVID-19 infection were reported among pregnant women and parturients admitted to Liangzhou Hospital. The source of infection was effectively controlled, ensuring the safety of the patients.

Conclusion: This study demonstrates the significance of improving pre-check and triage efficiency, strengthening the management of medical staff, and implementing specialized measures for pregnant women, parturients, and neonates to ensure their safety during the COVID-19 pandemic. The established protocols and procedures can serve as a valuable reference for other healthcare facilities seeking to enhance their pandemic prevention and control strategies in gynecology and obstetrics settings.

研究目的本研究旨在提高妇产科预检和分诊程序的效率,同时确保 COVID-19 大流行期间母婴患者的安全:2020年3月至2022年7月期间,优化了妇产科的工作流程,加强了对门诊、病房和产科病房医务人员的管理。制定并实施了针对孕妇、产妇和新生儿的特别方案。在严格遵守大流行预防和控制措施的前提下,为病人从门诊区到病房的流动制定了详细的程序和路线。采用信息化方法跟踪和监测高危孕妇、产妇及其家属的健康状况,促进准确、高效的预检和分流流程:结果:这些措施的实施取得了良好的效果。结果:这些措施的实施取得了良好的效果。在凉州医院住院的孕妇和产妇中未发现 COVID-19 感染病例。感染源得到有效控制,确保了患者的安全:本研究表明,在 COVID-19 大流行期间,提高预检分诊效率、加强医务人员管理、对孕妇、产妇和新生儿采取专门措施以确保其安全具有重要意义。所制定的协议和程序可为其他医疗机构加强妇产科大流行防控策略提供有价值的参考。
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引用次数: 0
Uterine Artery Doppler Parameters: An Excellent Tool for Assessing Adverse Pregnancy Outcomes. 子宫动脉多普勒参数:评估不良妊娠结局的绝佳工具。
IF 1.9 4区 医学 Q3 INTEGRATIVE & COMPLEMENTARY MEDICINE Pub Date : 2025-01-01
Minsui Cai, Yan Lu, Ying Li, Qi Cui

Objective: ObjectiveThis study aims to assess the utility of uterine artery Doppler parameters in predicting adverse pregnancy outcomes among pregnant women, thereby enhancing the safety of pregnancy.

Methods: This study utilized a prospective observational design. A total of 142 pregnant women who underwent prenatal ultrasonography at our hospital and Wuxi Maternal and Child Health Hospital (Maternity Hospital affiliated with Jiangnan University) from May 2022 to May 2023 were included. Uterine artery Doppler ultrasonography was performed between 11-22 weeks of gestation, and the pulsatility index (PI) and resistance index (RI) were determined. Patients were followed until delivery, and outcomes were categorized into safety and risk groups. Differences in resistance index and pulsatility index between the groups were analyzed, along with their impact on adverse pregnancy outcomes.

Results: Among the participants, 34 experienced adverse pregnancy outcomes. The risk group exhibited a higher resistance index and pulsatility index compared to the safety group (P < .05). The receiver operating characteristic curve analysis indicated that the resistance index had a sensitivity of 64.71% and specificity of 93.52% for adverse pregnancy outcomes (P < .05), while the sensitivity and specificity of the pulsatility index were 67.65% and 78.70%, respectively (P < .05). Within the subset of women with adverse outcomes, those with hypertensive disorders of pregnancy and/or gestational diabetes mellitus displayed the highest resistance index and pulsatility index (P < .05). Logistic regression analysis identified the resistance index and pulsatility index as independent risk factors for adverse pregnancy outcomes (P < .05).

Conclusions: Uterine artery Doppler parameters demonstrate excellent predictive value for adverse pregnancy outcomes in pregnant women. This underscores their potential in enhancing prenatal care and pregnancy management strategies.

研究目的本研究旨在评估子宫动脉多普勒参数在预测孕妇不良妊娠结局方面的实用性,从而提高妊娠安全性:本研究采用前瞻性观察设计。纳入2022年5月至2023年5月在我院和无锡市妇幼保健院(江南大学附属妇产医院)接受产前超声检查的142名孕妇。在孕 11-22 周时进行子宫动脉多普勒超声检查,并测定搏动指数(PI)和阻力指数(RI)。对患者进行随访直至分娩,并将结果分为安全组和风险组。对各组间阻力指数和脉动指数的差异及其对不良妊娠结局的影响进行了分析:结果:参与者中有 34 人出现不良妊娠结局。与安全组相比,风险组的阻力指数和脉动指数更高(P < .05)。接收器操作特征曲线分析表明,阻力指数对不良妊娠结局的敏感性为 64.71%,特异性为 93.52%(P < .05),而脉动指数的敏感性和特异性分别为 67.65% 和 78.70%(P < .05)。在出现不良妊娠结局的妇女子集中,患有妊娠高血压和/或妊娠糖尿病的妇女的阻力指数和脉动率指数最高(P < .05)。逻辑回归分析确定阻力指数和搏动指数是不良妊娠结局的独立风险因素(P < .05):结论:子宫动脉多普勒参数对孕妇不良妊娠结局具有极高的预测价值。结论:子宫动脉多普勒参数对孕妇的不良妊娠结局具有极高的预测价值,这凸显了它们在加强产前护理和妊娠管理策略方面的潜力。
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引用次数: 0
Study on the Relationship Between β2 Macroglobulin, Small Density, Low-density Lipoprotein and Carotid Plaque Instability after Acute Thrombolysis in Patients with Ischemic Stroke. 缺血性脑卒中患者急性溶栓后β2 巨球蛋白、小密度、低密度脂蛋白与颈动脉斑块不稳定性的关系研究
IF 1.9 4区 医学 Q3 INTEGRATIVE & COMPLEMENTARY MEDICINE Pub Date : 2025-01-01
Hao Zhang, Nini Yang, Mengke Ma, Wen-An Wang

Objective: Study the relationship between β2 microglobulin, small density, low-density lipoprotein and carotid plaque instability after acute thrombolysis in ischemic stroke patients (IS).

Methods: 319 patients with acute cerebral infarction who were treated by thrombolysis in the Department of Neurology, Chongming Branch of Shanghai Xinhua Hospital from January 2017 to May 2022 were included retrospectively. All subjects have undergone a carotid artery ultrasound examination for plaque. According to the ultrasound results, the subjects were divided into plaque-free group (94 cases), a stable plaque group (38 cases) and an unstable plaque group (187 cases). Use an automatic blood biochemical analyzer to detect routine indicators. At the same time, compare the differences of risk factors and biochemical indicators among the groups according to the demographic data of the patient's previous hospitalization. To further evaluate the related risk factors of the instability of carotid plaque in patients through the multivariate logistic regression analysis, the odds ratio (OR) and 95% confidence interval (95% CI) were calculated. Analysis the predictive value of β2 microglobulin and small density low density lipoprotein on the instability of carotid plaque in I.S. patients after acute thrombolysis through subject work characteristic curve (ROC).

Results: Among 319 patients, 187 had unstable plaque accounting for 58.6% and 38 had stable plaque accounting for 11.9%, according to the comparison of general clinical data. Lymphocyte, neutrophil ratio, triglyceride, T3, Hcy, β2 microglobulin has statistical significance in the presence or absence of plaque. Lymphocytes, small dense low-density lipoprotein, β2 microglobulin have statistical significance in the stability of plaque (P < .05). Total cholesterol, hypertension, β2 microglobulin and small density low-density lipoprotein may be independent risk factors of carotid plaque instability through multivariate logistic regression analysis (P < .05). The area under ROC curve showed that β2 microglobulin AUC: 0.6388, P < .05, small density low-density lipoprotein AUC: 0.6086, P < .05, combined diagnosis AUC: 0.6924, P < .05.

Conclusion: β2 microglobulin and density low-density lipoprotein are independent risk factors of carotid artery plaque instability in I.S. patients after acute thrombolysis. Moreover, the sensibility and differential of combined diagnosis are higher, which has certain predictive value for the instability of carotid plaque in such patients.

研究目的研究缺血性脑卒中患者(IS)急性溶栓后β2微球蛋白、小密度、低密度脂蛋白与颈动脉斑块不稳定性的关系。方法:回顾性纳入2017年1月至2022年5月在上海新华医院崇明分院神经内科接受溶栓治疗的319例急性脑梗死患者。所有受试者均接受了颈动脉超声斑块检查。根据超声检查结果,受试者被分为无斑块组(94例)、稳定斑块组(38例)和不稳定斑块组(187例)。使用自动血液生化分析仪检测常规指标。同时,根据患者既往住院的人口统计学数据,比较各组间危险因素和生化指标的差异。通过多变量逻辑回归分析,进一步评估患者颈动脉斑块不稳定的相关危险因素,计算几率比(OR)和95%置信区间(95% CI)。通过受试者工作特征曲线(ROC)分析β2微球蛋白和小密度低密度脂蛋白对急性溶栓后I.S.患者颈动脉斑块不稳定性的预测价值:319例患者中,根据一般临床数据比较,187例为不稳定斑块,占58.6%;38例为稳定斑块,占11.9%。淋巴细胞、中性粒细胞比率、甘油三酯、T3、Hcy、β2 微球蛋白与是否存在斑块有统计学意义。淋巴细胞、小致密低密度脂蛋白、β2 微球蛋白对斑块的稳定性有统计学意义(P < .05)。通过多变量逻辑回归分析,总胆固醇、高血压、β2微球蛋白和小密度低密度脂蛋白可能是颈动脉斑块不稳定的独立危险因素(P < .05)。ROC 曲线下面积显示,β2 微球蛋白 AUC:0.6388, P < .05, 小密度低密度脂蛋白 AUC:0.6086,P < .05;综合诊断 AUC:0.6924,P < .05:结论:β2 微球蛋白和低密度脂蛋白是急性溶栓后 I.S. 患者颈动脉斑块不稳定的独立危险因素。此外,联合诊断的敏感性和鉴别度较高,对此类患者颈动脉斑块的不稳定性有一定的预测价值。
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引用次数: 0
Retrieval of Trophoblast Cells from The Robertsonian Translocations for Prenatal Diagnosis. 从罗伯逊易位中提取滋养层细胞用于产前诊断
IF 1.9 4区 医学 Q3 INTEGRATIVE & COMPLEMENTARY MEDICINE Pub Date : 2025-01-01
Zhen Xu, Sheng Li, Long He

Objective: To provide genetic information about the fetuses from carriers of Robertsonian (Rob) translocation and to explore the application value of extravillous trophoblasts (EVTs) cells collected from the cervical canal for prenatal diagnosis.

Method: Trophoblast retrieval and isolation from the cervix (TRIC) is an approach that non-invasively isolates homogeneous trophoblast cells. In this study, the EVT cells were collected from the cervix of 20 pregnant women between 5-7 weeks gestation. Thereafter, STR analysis and fluorescence in situ hybridization (FISH) were performed on these trophoblast cells.

Results: In 1 case (P5), we failed to collect the trophoblast cells, STR analysis showed maternal cell contamination in 4 cases, 6 cases were normal/balanced chromosome, and 9 cases were associated with unbalanced chromosome. The results of these 15 cases were consistent with those of villi FISH examination or cytogenetic analysis of cultured amniocytes.

Conclusion: The collection of fetal trophoblast cells from the cervix provides a feasible approach for prenatal diagnosis. Rob translocation homozygosity could be seen as a potential means of speciation in humans with 44 chromosomes.

目的提供罗伯逊(Rob)易位携带者胎儿的遗传信息,并探讨从宫颈管收集的绒毛外滋养层细胞(EVTs)在产前诊断中的应用价值:方法:宫颈滋养层细胞检索和分离(TRIC)是一种无创分离均质滋养层细胞的方法。本研究从 20 名妊娠 5-7 周的孕妇宫颈中收集了 EVT 细胞。随后,对这些滋养层细胞进行了STR分析和荧光原位杂交(FISH):结果:1 例(P5)未能采集到滋养层细胞,4 例的 STR 分析显示母体细胞受到污染,6 例为正常/平衡染色体,9 例伴有不平衡染色体。这 15 个病例的结果与绒毛 FISH 检查或培养羊膜细胞的细胞遗传学分析结果一致:结论:从宫颈收集胎儿滋养层细胞为产前诊断提供了一种可行的方法。Rob 易位同源染色体可被视为人类 44 条染色体分化的一种潜在手段。
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引用次数: 0
Effectiveness of Meticulous Management in Reducing Hospital-Acquired Infections in Neonatal Wards: A Prospective Analysis. 精细化管理在减少新生儿病房医院获得性感染方面的效果:前瞻性分析。
IF 1.9 4区 医学 Q3 INTEGRATIVE & COMPLEMENTARY MEDICINE Pub Date : 2025-01-01
Hongyu Chen, Zhuan Miao, Yarong Cai, Le Dong

Objective: To investigate the effect of meticulous management on infection prevention and infection rate control in neonatal wards.

Methods: 230 neonates admitted to Northwest Women and Children's Hospital from January 2022 to June 2022 were selected as the control group and 230 neonates admitted to Northwest Women and Children's Hospital in the same period were selected as the study group. The control group was given routine management, and the study group was given meticulous management. The sanitary conditions of the items in the wards, the rate of implementation of standard disinfection measures, neonatal infections, and the satisfaction of neonatal guardians were compared between the two groups.

Results: The qualified rates of air, nipple, bottle, disinfectants, sterilized items, and hand hygiene of medical staff in the study group were higher than those in the control group (P < .05). The implementation rates of environmental management, equipment disinfection, personnel management, and skin management disinfection in the neonatal ward of the study group were higher than those of the control group (P < .05). The total infection rate of all newborns in the study group was lower than that of the control group (P < .05). Total satisfaction was higher in the study group than in the control group (P < .05).

Conclusion: Meticulous management can play an effective role in the prevention and control of hospital-acquired infections in neonatal wards, and can effectively reduce the infection rate, which can be promoted and applied in clinical settings.

目的:探讨精细化管理对新生儿病房感染预防及感染率控制的影响:方法:选取2022年1月-2022年6月西北妇女儿童医院收治的230例新生儿作为对照组,选取同期西北妇女儿童医院收治的230例新生儿作为研究组。对照组给予常规管理,研究组给予精细化管理。比较两组病房物品的卫生状况、标准消毒措施的执行率、新生儿感染情况以及新生儿监护人的满意度:研究组医务人员空气、乳头、奶瓶、消毒剂、消毒物品、手卫生的合格率均高于对照组(P<0.05)。研究组新生儿病房环境管理、设备消毒、人员管理和皮肤管理消毒的实施率均高于对照组(P < .05)。研究组所有新生儿的总感染率低于对照组(P < .05)。研究组的总满意度高于对照组(P < .05):精细化管理可在预防和控制新生儿病房院内感染中发挥有效作用,并能有效降低感染率,可在临床中推广应用。
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引用次数: 0
The Maternal-Fetal Risk Factors of Hypertensive Disorders of Pregnancy and its Effects on Infant Complete Blood Count and Coagulation Factors. 妊娠高血压疾病的母胎风险因素及其对婴儿全血细胞计数和凝血因子的影响。
IF 1.9 4区 医学 Q3 INTEGRATIVE & COMPLEMENTARY MEDICINE Pub Date : 2025-01-01
Shurong Wang, Tian Sang, Zhaona Li, Jianrong Ma

Objective: Our aim was to analyze the risk factors of hypertensive disorders of pregnancy (HDP) and explore its influence on fetal risk factors, infant's blood cells and markers of inflammation.

Methods: A total of 123 patients with HDP were in the HDP group, and 121 healthy pregnant women were selected as the control group. The general clinical data of the participants were recorded. Statistics of maternal and infant outcomes, delivery methods, routine blood lab results and coagulation factors of the newborn were recorded. Univariate analysis and multi-factor analysis were used to explore the risk factors for HDP.

Results: The overall incidence of poor maternal outcomes in the HDP group was higher than in the control group. The incidence of premature delivery; postpartum hemorrhage; coagulopathy; placental abruption; heart failure and hemolysis, elevated liver enzymes, and low platelets (HELLP) syndrome was significantly higher in the HDP group than in the control group (P < .05). The cesarean section rate in the HDP group was significantly higher than in the control group (P < .05). The overall incidence of poor outcomes in fetuses and newborns in the HDP group was higher than in the control group. The incidence of infant low birth weight, intraventricular hemorrhage (IVH), neonatal respiratory distress syndrome (NRDS), asphyxia and all-cause neonatal death were higher than in the control group (P < .05). The incidence of small gestational age (SGA), fetal distress and intrauterine death in the HDP group were higher than in the control group (P < .05). In the HDP group, neonatal white blood cells (WBC), neutrophils (NEUT) and platelets (PLT) were significantly lower than in the control group (P < .05), while hemoglobin (Hgb) and hematocrit (Hct) were higher (P < .05).

Conclusions: HDP endangers the health of mother and infant; Age, body mass index (BMI) (>24 kg/m2), parity, history of hypertension, family history of hypertension and other factors may be involved in the occurrence and development of HDP.

目的:分析妊娠高血压疾病(HDP)的风险因素,探讨其对胎儿风险因素、婴儿血细胞和炎症指标的影响:我们的目的是分析妊娠高血压疾病(HDP)的危险因素,并探讨其对胎儿危险因素、婴儿血细胞和炎症指标的影响:方法:共选取 123 名妊娠高血压疾病患者为妊娠高血压疾病组,121 名健康孕妇为对照组。记录参与者的一般临床数据。记录母婴结局、分娩方式、血常规化验结果和新生儿凝血因子的统计数据。采用单变量分析和多因素分析来探讨 HDP 的风险因素:结果:HDP 组产妇不良结局的总体发生率高于对照组。HDP组早产、产后出血、凝血功能障碍、胎盘早剥、心力衰竭、溶血、肝酶升高和血小板低(HELLP)综合征的发生率明显高于对照组(P < .05)。HDP 组的剖宫产率明显高于对照组(P < .05)。HDP 组胎儿和新生儿不良结局的总体发生率高于对照组。婴儿低出生体重、脑室内出血(IVH)、新生儿呼吸窘迫综合征(NRDS)、窒息和新生儿全因死亡的发生率均高于对照组(P < .05)。HDP 组的小胎龄(SGA)、胎儿窘迫和宫内死亡发生率高于对照组(P < .05)。HDP 组新生儿白细胞(WBC)、中性粒细胞(NEUT)和血小板(PLT)明显低于对照组(P < .05),而血红蛋白(Hgb)和血细胞比容(Hct)则高于对照组(P < .05):HDP危及母婴健康;年龄、体重指数(BMI)(大于 24 kg/m2)、胎次、高血压病史、高血压家族史等因素可能与 HDP 的发生和发展有关。
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引用次数: 0
Comparing Effects of Aerobic Versus Resistance Exercise on Expression of MicroRNA-155, Serum- and Glucocorticoid-Regulated Kinase 3, and Pain Threshold in Mice with Experimental Autoimmune Encephalomyelitis. 比较有氧运动与阻力运动对实验性自身免疫性脑脊髓炎小鼠体内微RNA-155、血清和糖皮质激素调节激酶3的表达以及疼痛阈值的影响
IF 1.9 4区 医学 Q3 INTEGRATIVE & COMPLEMENTARY MEDICINE Pub Date : 2025-01-01
Mina Ahmadi Kakavandi, Shahnaz Shahrbanian, Mohammad Reza Kordi, Bahram Mohammad Soltani

Objectives: Multiple sclerosis is a complex neurological disorder in which the immune system attacks the myelin sheath, leading to a range of symptoms. microRNA-155 plays a significant role in the pathogenesis of multiple sclerosis and neuropathic pain. Serum- and glucocorticoid-regulated kinase 3 (SGK3) protein is the target of microRNA-155. Exercise can induce long-term changes in the expression of microRNAs and their target proteins. This study aims to compare the effects of resistance and aerobic exercises on the expression of microRNA-155, SGK3, and pain threshold in experimental autoimmune encephalomyelitis mice.

Methods: For this purpose, 24 female C57BL6 mice were divided into four groups using a simple random sampling method: healthy, experimental autoimmune encephalomyelitis, resistance exercise + experimental autoimmune encephalomyelitis, and aerobic exercise + experimental autoimmune encephalomyelitis. The exercise groups underwent four weeks of training, consisting of 5 sessions of 30 minutes per week. The expression of microRNA-155, SGK3 protein, and pain threshold were assessed using the real-time polymerase chain reaction method, Western blot analysis, and the tail flick test, respectively.

Results: The results indicated significant changes in the expression of microRNA-155 (F = 22.05, P = .0003), SGK3 (F = 26.70, P = .0002), and pain threshold (F = 5.05, P = .009). Both resistance and aerobic exercises significantly down-regulated microRNA-155 and increased the expression of SGK3 compared to the experimental autoimmune encephalomyelitis control group (P < .05). The pain threshold in the mice also increased in both exercise groups (P < .05). No significant differences were observed between the resistance and aerobic exercise groups in any outcomes (P > .05).

Conclusion: Exercise training, through its impact on epigenetic factors, may serve as an effective therapeutic intervention to alleviate pain in individuals with multiple sclerosis and could significantly manage disease symptoms.

研究目的microRNA-155在多发性硬化症和神经性疼痛的发病机制中发挥着重要作用。血清和糖皮质激素调节激酶 3(SGK3)蛋白是 microRNA-155 的靶标。运动可诱导 microRNA 及其靶蛋白表达的长期变化。本研究旨在比较阻力运动和有氧运动对实验性自身免疫性脑脊髓炎小鼠microRNA-155、SGK3和痛阈表达的影响:为此,采用简单随机抽样法将 24 只雌性 C57BL6 小鼠分为四组:健康组、实验性自身免疫性脑脊髓炎组、阻力运动 + 实验性自身免疫性脑脊髓炎组和有氧运动 + 实验性自身免疫性脑脊髓炎组。运动组进行了为期四周的训练,每周 5 次,每次 30 分钟。分别采用实时聚合酶链反应法、Western印迹分析法和尾弹试验评估了microRNA-155、SGK3蛋白的表达和痛阈:结果表明,microRNA-155(F = 22.05,P = .0003)、SGK3(F = 26.70,P = .0002)和痛阈(F = 5.05,P = .009)的表达均有明显变化。与实验性自身免疫性脑脊髓炎对照组相比,阻力运动和有氧运动都能明显下调 microRNA-155 的表达,并增加 SGK3 的表达(P < .05)。两组小鼠的痛阈值也都有所提高(P < .05)。阻力运动组和有氧运动组在任何结果上都没有观察到明显差异(P > .05):运动训练通过对表观遗传因素的影响,可作为一种有效的治疗干预措施,缓解多发性硬化症患者的疼痛,并能显著控制疾病症状。
{"title":"Comparing Effects of Aerobic Versus Resistance Exercise on Expression of MicroRNA-155, Serum- and Glucocorticoid-Regulated Kinase 3, and Pain Threshold in Mice with Experimental Autoimmune Encephalomyelitis.","authors":"Mina Ahmadi Kakavandi, Shahnaz Shahrbanian, Mohammad Reza Kordi, Bahram Mohammad Soltani","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objectives: </strong>Multiple sclerosis is a complex neurological disorder in which the immune system attacks the myelin sheath, leading to a range of symptoms. microRNA-155 plays a significant role in the pathogenesis of multiple sclerosis and neuropathic pain. Serum- and glucocorticoid-regulated kinase 3 (SGK3) protein is the target of microRNA-155. Exercise can induce long-term changes in the expression of microRNAs and their target proteins. This study aims to compare the effects of resistance and aerobic exercises on the expression of microRNA-155, SGK3, and pain threshold in experimental autoimmune encephalomyelitis mice.</p><p><strong>Methods: </strong>For this purpose, 24 female C57BL6 mice were divided into four groups using a simple random sampling method: healthy, experimental autoimmune encephalomyelitis, resistance exercise + experimental autoimmune encephalomyelitis, and aerobic exercise + experimental autoimmune encephalomyelitis. The exercise groups underwent four weeks of training, consisting of 5 sessions of 30 minutes per week. The expression of microRNA-155, SGK3 protein, and pain threshold were assessed using the real-time polymerase chain reaction method, Western blot analysis, and the tail flick test, respectively.</p><p><strong>Results: </strong>The results indicated significant changes in the expression of microRNA-155 (F = 22.05, P = .0003), SGK3 (F = 26.70, P = .0002), and pain threshold (F = 5.05, P = .009). Both resistance and aerobic exercises significantly down-regulated microRNA-155 and increased the expression of SGK3 compared to the experimental autoimmune encephalomyelitis control group (P < .05). The pain threshold in the mice also increased in both exercise groups (P < .05). No significant differences were observed between the resistance and aerobic exercise groups in any outcomes (P > .05).</p><p><strong>Conclusion: </strong>Exercise training, through its impact on epigenetic factors, may serve as an effective therapeutic intervention to alleviate pain in individuals with multiple sclerosis and could significantly manage disease symptoms.</p>","PeriodicalId":7571,"journal":{"name":"Alternative therapies in health and medicine","volume":" ","pages":"28-34"},"PeriodicalIF":1.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142556905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Protective Efficacy of T-type, Calcium Channel Antagonist on Auditory Function in Cdh23 Erl/Erl Mice. T 型钙通道拮抗剂对 Cdh23 Erl/Erl 小鼠听觉功能的保护作用
IF 1.9 4区 医学 Q3 INTEGRATIVE & COMPLEMENTARY MEDICINE Pub Date : 2025-01-01
Weijun Ma, Heng Li, Juan Hu, Ying Gao, Xiaotong Zhang, Min Xu, Ying Cheng
<p><strong>Context: </strong>In normal physiological conditions, calcium ions (Ca2+) have an important effect in terms of the regulation of hair cell (HC) functions, and T-type calcium antagonists may be protective against hearing loss. However, no studies have occurred related to a T-type calcium-channel antagonist with regard to otoprotection in a Cdh23 mouse model.</p><p><strong>Objective: </strong>The study intended to examine the protective efficacy of ethosuximide-a T-type calcium-channel antagonist-against age-related hearing loss in a Cadherin 23 (Cdh23) erl/erl mouse model, to potentially offer an insight and foundation for therapy in the near future for patients in clinical practice who suffer from age-related hearing loss.</p><p><strong>Design: </strong>The research team conducted an animal study.</p><p><strong>Animals: </strong>The animals were 12 male and 12 female, Cdh23 erl/erl mice.</p><p><strong>Intervention: </strong>The research team randomly divided the mice into two groups, with 12 mice in each group: (1) the control group, the saline group, which received saline at 10 mL/kg of body weight, and (2) the intervention group, the ethosuximide group, which received ethosuximide at 10 mL/kg of body weight. Both groups received the treatments intraperitoneally every other day, beginning postnatally at 7 days until the mice were 8 weeks old.</p><p><strong>Outcome measures: </strong>For both groups, the research team: (1) measured auditory brainstem response (ABR); (2) measured distortion product otoacoustic emission (DPOAE) at 4, 6, and 8 weeks of age; (3) separated the basilar membrane from the modiolus and lateral tissues and determined the percentage of inner-and-outer hair-cell (IHC and OHC) loss; (4) investigated relative levels of apoptosis-related gene mRNA using reverse transcription polymerase chain reaction (PCR); and (5) examined relative levels of apoptosis-related protein, using immunofluorescent (IF) staining.</p><p><strong>Results: </strong>Compared to the saline group, the ethosuximide group: (1) had significantly lower ABR thresholds for click at 8 weeks, for 8 KHz at 6 and 8 weeks, and for 16 KHz and 32 KHz (all P < .01); (2) had significantly higher DPOAE amplitudes at 4 weeks at 15.4 KHz and 17.7 KHz (both P < .01); at 6 weeks at 8.8 KHz (P < .05), 10.1 KHz (P < .01), 11.7 KHz (P < .01), 13.4 KHz (P < .01), 15.4 KHz (P < .01), and 17.7 KHz (P < .01); and at 8 weeks at 6.7 KHz (P < .05), 7.7 KHz (P < .05), 10.1 KHz (P < .01), 11.7 KHz (P < .01), 13.4 KHz (P < .01), 15.4 KHz (P < .01), and 17.7 KHz (P < .01); (3) had significantly lower OHC loss in the middle and basal turns of the cochlea's surface (both P < .05); (4) at the age of 2 months, had significantly lower mRNA relative levels of apoptosis-related genes, including caspase-3, caspase-9, caspase-12, m-calpain and u-calpain, as found using a polymerase chain reaction (PCR); and (5) had weaker protein levels of caspase-3 and caspase-9 in the inner ears, as fou
背景:在正常生理条件下,钙离子(Ca2+)在调节毛细胞(HC)功能方面具有重要作用,T型钙离子拮抗剂可能对听力损失具有保护作用。然而,目前还没有关于 T 型钙通道拮抗剂在 Cdh23 小鼠模型中的耳保护作用的研究:本研究旨在探讨乙琥胺--一种 T 型钙通道拮抗剂--在 Cadherin 23 (Cdh23) erl/erl 小鼠模型中对老年性听力损失的保护作用,以便在不久的将来为临床上患有老年性听力损失的患者的治疗提供启示和依据:研究小组进行了一项动物研究:动物:12 只雌雄 Cdh23 erl/erl 小鼠:研究小组将小鼠随机分为两组,每组 12 只:(1) 对照组,即生理盐水组,按每公斤体重 10 毫升的剂量接受生理盐水治疗;(2) 干预组,即乙琥胺组,按每公斤体重 10 毫升的剂量接受乙琥胺治疗。两组小鼠均从出生后7天开始,每隔一天腹腔注射一次治疗药物,直至小鼠8周大:对于两组小鼠,研究小组(1) 测量听性脑干反应(ABR);(2) 在小鼠 4、6 和 8 周龄时测量畸变产物耳声发射(DPOAE);(3) 将基底膜与模耳和外侧组织分离,并确定内毛细胞和外毛细胞(IHC 和 OHC)损失的百分比;(4) 使用反转录聚合酶链反应(PCR)检测与细胞凋亡相关的基因 mRNA 的相对水平;以及 (5) 使用免疫荧光(IF)染色检测与细胞凋亡相关的蛋白质的相对水平。结果与生理盐水组相比,乙琥胺组:(1) 8周时点击的ABR阈值、6周和8周时8 KHz的ABR阈值、16 KHz和32 KHz的ABR阈值均显著降低(均P < .01);(2) 4周时15.4 KHz和17.7 KHz的DPOAE振幅显著升高(均P < .01);(3) 4周时16 KHz和32 KHz的DPOAE振幅显著降低(均P < .01)。7 KHz(均 P < .01);6 周时,8.8 KHz(P < .05)、10.1 KHz(P < .01)、11.7 KHz(P < .01)、13.4 KHz(P < .01)、15.4 KHz(P < .01)和 17.7 KHz(P < .01);8 周时为 6.7 KHz(P < .05)、7.7 KHz(P < .05)、10.1 KHz(P < .01)、11.7 KHz(P < .01)、13.4 KHz (P < .01)、15.4 KHz (P < .01) 和 17.7 KHz (P < .01);(3) 耳蜗表面中部和基底转折处的 OHC 损失显著较低(均 P < .05);(4)在 2 个月大时,使用聚合酶链式反应(PCR)发现,与细胞凋亡相关的基因(包括 caspase-3、caspase-9、caspase-12、m-calpain 和 u-calpain)的 mRNA 相对水平明显较低;(5)使用免疫荧光(IF)染色发现,内耳中 caspase-3 和 caspase-9 的蛋白水平较低:结论:T型钙通道拮抗剂对cdh23 erl/erl小鼠的听力功能有保护作用。
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引用次数: 0
Study on the Impact of Expectations and Beliefs in Distance Initiation Experiences in Reiki. 研究灵气疗法远程启动体验中的期望和信念的影响。
IF 1.9 4区 医学 Q3 INTEGRATIVE & COMPLEMENTARY MEDICINE Pub Date : 2025-01-01
Scarascia Graziano, Cristiano Luigi

Background: Reiki is a natural healing technique in which the initiation rite, or attunement, is crucial to its teaching. Remote initiation has become a common practice in distance learning courses.

Primary objective: The purpose of this study is to examine the subjective experience of a group of people who were told to expect remote initiation as part of an online Reiki course but did not receive it, and analyse their responses and possible placebo effects associated with this experience.

Methods/design: A heterogeneous group of 94 participants (N = 94) was chosen which grouped people who had never received a Reiki initiation and people already trained but repeating the first level training. Data were collected using a 58-question questionnaire and were analysed using descriptive statistics, including frequencies, averages, and standard deviations.

Results: This study shows that beliefs and expectations strongly influenced participants' perceptions and feelings during the initiation, regardless of their prior Reiki experience. In addition, even individuals with little experience can have comparable experiences as others. Overall, the study highlights the role of bias and preconceptions in subjective experiences during Reiki initiations.

Conclusion: Even without formal initiation, participants reported sensations associated with it, such as warmth, tingling, and emotional responses. Psychological factors, such as expectations and cognitive biases, play a significant role in shaping these experiences. More research is needed on placebo effects and belief in Reiki.

背景介绍灵气(Reiki)是一种自然疗法,其启动仪式或调适(attunement)对其教学至关重要。远程启动已成为远程学习课程中的一种常见做法:本研究的目的是考察一组被告知远程启动仪式是在线灵气课程的一部分但并未接受远程启动仪式的人的主观体验,并分析他们的反应以及与这种体验相关的可能的安慰剂效应:选择了一个由 94 名参与者组成的异质群体(N = 94),其中包括从未接受过灵气启动的人和已经接受过培训但正在重复第一级培训的人。使用 58 个问题的问卷收集数据,并使用描述性统计方法进行分析,包括频率、平均值和标准偏差:研究结果表明,无论参与者以前是否有过灵气训练经验,信念和期望都会强烈影响他们在灵气训练过程中的感知和感受。此外,即使是经验不足的人也能获得与其他人相似的体验。总之,这项研究强调了偏见和先入之见在灵气启动过程中对主观体验的影响:即使没有正式启动,参与者也会报告与之相关的感觉,如温暖、刺痛和情绪反应。心理因素,如期望和认知偏差,在形成这些体验方面起着重要作用。还需要对灵气的安慰剂效应和信仰进行更多的研究。
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Alternative therapies in health and medicine
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