Pub Date : 1975-01-01DOI: 10.1007/978-1-4613-4440-7_31
S C Weddington, P Brandtzaeg, K Sletten, T Christensen, V Hansson
Testicular androgen binding protein (ABP) was purified from the epididymis of 1500 adult rabbits by the sequential use of ammonium sulphate precipitation, ion exchange chromatography on DEAE cellulose, gel filtration on Sephadex G-200, hydroxyl-apatite chromatography and preparative polyacrylamide gel electrophoresis. This procedure yielded a 1000-fold increase in specific activity compared to that of the 1500,000 x g supernatant, and the recovery of active ABP was about 3-5%. ABP is acid glycoprotein with a molecular weight of 65-68,000 daltons. Antisera to rabbit ABP raised in quinea pigs inhibit 3H-DHT binding to ABP as measured by SS-PAGE. When diluted rabbit serum containing TeBG is treated with the same dilutions of these antisera, identical binding inhibition curves are found. Thus, ABP and TeBG in rabbits appear to possess identical immunological determinants.
{"title":"Purification and characterization of rabbit testicular androgen binding protein (ABP).","authors":"S C Weddington, P Brandtzaeg, K Sletten, T Christensen, V Hansson","doi":"10.1007/978-1-4613-4440-7_31","DOIUrl":"https://doi.org/10.1007/978-1-4613-4440-7_31","url":null,"abstract":"<p><p>Testicular androgen binding protein (ABP) was purified from the epididymis of 1500 adult rabbits by the sequential use of ammonium sulphate precipitation, ion exchange chromatography on DEAE cellulose, gel filtration on Sephadex G-200, hydroxyl-apatite chromatography and preparative polyacrylamide gel electrophoresis. This procedure yielded a 1000-fold increase in specific activity compared to that of the 1500,000 x g supernatant, and the recovery of active ABP was about 3-5%. ABP is acid glycoprotein with a molecular weight of 65-68,000 daltons. Antisera to rabbit ABP raised in quinea pigs inhibit 3H-DHT binding to ABP as measured by SS-PAGE. When diluted rabbit serum containing TeBG is treated with the same dilutions of these antisera, identical binding inhibition curves are found. Thus, ABP and TeBG in rabbits appear to possess identical immunological determinants.</p>","PeriodicalId":75766,"journal":{"name":"Current topics in molecular endocrinology","volume":"2 ","pages":"433-51"},"PeriodicalIF":0.0,"publicationDate":"1975-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12418963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1975-01-01DOI: 10.1007/978-1-4613-4440-7_34
P. S. Tung, I. Fritz
{"title":"Follicle stimulating hormone and cyclic AMP directed changes in ultrastructure and properties of cultured Sertoli cell-enriched preparations: comparison with cultured testicular peritubular cells.","authors":"P. S. Tung, I. Fritz","doi":"10.1007/978-1-4613-4440-7_34","DOIUrl":"https://doi.org/10.1007/978-1-4613-4440-7_34","url":null,"abstract":"","PeriodicalId":75766,"journal":{"name":"Current topics in molecular endocrinology","volume":"76 2","pages":"459-508"},"PeriodicalIF":0.0,"publicationDate":"1975-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50971445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1975-01-01DOI: 10.1007/978-1-4613-4440-7_10
H W Baker, C W Bardin, G J Eichner, L S Jefferson, R J Santen
{"title":"Perfusion of the male rat reproductive tract: a model for the study of androgen secretion and action.","authors":"H W Baker, C W Bardin, G J Eichner, L S Jefferson, R J Santen","doi":"10.1007/978-1-4613-4440-7_10","DOIUrl":"https://doi.org/10.1007/978-1-4613-4440-7_10","url":null,"abstract":"","PeriodicalId":75766,"journal":{"name":"Current topics in molecular endocrinology","volume":"2 ","pages":"135-44"},"PeriodicalIF":0.0,"publicationDate":"1975-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12412684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Follicle stimulating hormone and cyclic AMP directed changes in ultrastructure and properties of cultured Sertoli cell-enriched preparations: comparison with cultured testicular peritubular cells.","authors":"P S Tung, I B Fritz","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":75766,"journal":{"name":"Current topics in molecular endocrinology","volume":"2 ","pages":"459-508"},"PeriodicalIF":0.0,"publicationDate":"1975-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11411703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1975-01-01DOI: 10.1007/978-1-4613-4440-7_18
A A Smith, W S McLean, S N Nayfeh, F S French
{"title":"Androgen receptor in rat testis.","authors":"A A Smith, W S McLean, S N Nayfeh, F S French","doi":"10.1007/978-1-4613-4440-7_18","DOIUrl":"https://doi.org/10.1007/978-1-4613-4440-7_18","url":null,"abstract":"","PeriodicalId":75766,"journal":{"name":"Current topics in molecular endocrinology","volume":"2 ","pages":"257-80"},"PeriodicalIF":0.0,"publicationDate":"1975-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12412689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1975-01-01DOI: 10.1007/978-1-4613-4440-7_20
E Mulder, M J Peters, H J van der Molen
{"title":"Androgen receptors in testis tissue enriched in Sertoli cells.","authors":"E Mulder, M J Peters, H J van der Molen","doi":"10.1007/978-1-4613-4440-7_20","DOIUrl":"https://doi.org/10.1007/978-1-4613-4440-7_20","url":null,"abstract":"","PeriodicalId":75766,"journal":{"name":"Current topics in molecular endocrinology","volume":"2 ","pages":"287-91"},"PeriodicalIF":0.0,"publicationDate":"1975-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12412691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Intra-arterial infusion of testosterone-3H gave rise to tritiated dihydrotestosterone, 5 alpha-androstan-3 alpha,17 beta-diol and 5 alpha-androstan-3beta,17 beta-diol in spermatic venous effluent of the perfused rabbit testis-epididymis. Mass spectrometric measurements confirmed that these four androgens were present in spermatic venous effluent of the perfused rabbit testis-epididymis. Gas liquid chromatographic measurement showed that testosterone, dihydrotestosterone, 5 alpha-androstan-3 alpha,17 beta-diol and 5 alpha-androstan-3 beta,17 beta-diol were secreted in similar amounts by the in vitro perfused and in situ rabbit testis-epididymis results obtained by perfusing the testis minus the epididymis suggested that the bulk of these androgens originate from the catabolism of testosterone within the testis rather than the epididymis. Suprisingly, germinal epithelium destruction by heat failed to alter the testosterone, dihydrotestosterone and 5 alpha-androstan-3 alpha,17 beta-diol secretion by the in vitro perfused rabbit testis. In contrast, the secretion of 5 alpha-androstan-3 beta,17 beta-diol was significantly (P less than 0.05) reduced in the same cryptorchid compared to control testes.
{"title":"Some factors affecting testosterone, dihydrotestosterone, 5 alpha-androstan-3 alpha,17 beta-diol and 5 alpha-androstan-3 beta,17 beta-diol secretion by invitro perfused rabbit testes.","authors":"L L Ewing","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Intra-arterial infusion of testosterone-3H gave rise to tritiated dihydrotestosterone, 5 alpha-androstan-3 alpha,17 beta-diol and 5 alpha-androstan-3beta,17 beta-diol in spermatic venous effluent of the perfused rabbit testis-epididymis. Mass spectrometric measurements confirmed that these four androgens were present in spermatic venous effluent of the perfused rabbit testis-epididymis. Gas liquid chromatographic measurement showed that testosterone, dihydrotestosterone, 5 alpha-androstan-3 alpha,17 beta-diol and 5 alpha-androstan-3 beta,17 beta-diol were secreted in similar amounts by the in vitro perfused and in situ rabbit testis-epididymis results obtained by perfusing the testis minus the epididymis suggested that the bulk of these androgens originate from the catabolism of testosterone within the testis rather than the epididymis. Suprisingly, germinal epithelium destruction by heat failed to alter the testosterone, dihydrotestosterone and 5 alpha-androstan-3 alpha,17 beta-diol secretion by the in vitro perfused rabbit testis. In contrast, the secretion of 5 alpha-androstan-3 beta,17 beta-diol was significantly (P less than 0.05) reduced in the same cryptorchid compared to control testes.</p>","PeriodicalId":75766,"journal":{"name":"Current topics in molecular endocrinology","volume":"2 ","pages":"145-64"},"PeriodicalIF":0.0,"publicationDate":"1975-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11241151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1975-01-01DOI: 10.1007/978-1-4613-4440-7_29
M Griswold, E Mably, I B Fritz
{"title":"Stimulation by follicle stimulating hormone and dibutyryl cyclic AMP of incorporation of 3H-thymidine into nuclear DNA of cultured Sertoli cell-enriched preparations from immature rats.","authors":"M Griswold, E Mably, I B Fritz","doi":"10.1007/978-1-4613-4440-7_29","DOIUrl":"https://doi.org/10.1007/978-1-4613-4440-7_29","url":null,"abstract":"","PeriodicalId":75766,"journal":{"name":"Current topics in molecular endocrinology","volume":"2 ","pages":"413-20"},"PeriodicalIF":0.0,"publicationDate":"1975-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/978-1-4613-4440-7_29","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11411702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1975-01-01DOI: 10.1007/978-1-4613-4440-7_28
A Steinberger, J S Elkington, B M Sanborn, E Steinberger
{"title":"Culture and FSH responses of Sertoli cells isolated from sexually mature rat testis.","authors":"A Steinberger, J S Elkington, B M Sanborn, E Steinberger","doi":"10.1007/978-1-4613-4440-7_28","DOIUrl":"https://doi.org/10.1007/978-1-4613-4440-7_28","url":null,"abstract":"","PeriodicalId":75766,"journal":{"name":"Current topics in molecular endocrinology","volume":"2 ","pages":"399-411"},"PeriodicalIF":0.0,"publicationDate":"1975-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11411701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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