A. Gugołek, J. Strychalski, J. Juśkiewicz, E. Żary-Sikorska
Chinchillas are herbivores, but wild chinchillas may occasionally consume animal-based foods. The aim of this study was to determine the effect of fish meal (FM) and mealworm meal (MWM) included in complete pelleted diets on nutrient digestibility and gastrointestinal function in chinchillas. The experiment was performed on 24 male, divided into three groups, n=8. Control group (C) was fed a diet containing 10% soybean meal (SBM). In the experimental group FM, chinchillas received a diet containing 3% fish meal, and the diet administered to the experimental group MWM was supplemented with 4% dried mealworm larvae meal. The nutrient digestibility of diets was determined. At the end of the experiment animals were euthanized and their digestive tracts were removed to analyze gut activity. FM group animals were characterized by lower crude fat digestibility, whereas both alternative protein sources improved the digestibility of acid detergent fiber (ADF). A considerable increase in the activity of cecal intracellular and extracellular bacterial enzymes (in particular β-glucosidase, β-galactosidase and β-xylosidase) was noted in the FM group, which however did not increase the concentrations of short-chain fatty acids (SCFA). The inclusion of MWM in chinchilla diets shifted the bacterial fermentation site from the cecum (lowest SCFA pool) to the colon (highest SCFA pool), thus enabling to derive additional energy from less digestible dietary components. In conclusion, chinchilla diets can be supplemented with small amounts of animal protein such as fish meal and dried mealworm larvae meal.
{"title":"The effect of fish and mealworm larvae meals as alternative dietary protein sources on nutrient digestibility and gastrointestinal function in Chinchilla lanigera","authors":"A. Gugołek, J. Strychalski, J. Juśkiewicz, E. Żary-Sikorska","doi":"10.1538/expanim.19-0072","DOIUrl":"https://doi.org/10.1538/expanim.19-0072","url":null,"abstract":"Chinchillas are herbivores, but wild chinchillas may occasionally consume animal-based foods. The aim of this study was to determine the effect of fish meal (FM) and mealworm meal (MWM) included in complete pelleted diets on nutrient digestibility and gastrointestinal function in chinchillas. The experiment was performed on 24 male, divided into three groups, n=8. Control group (C) was fed a diet containing 10% soybean meal (SBM). In the experimental group FM, chinchillas received a diet containing 3% fish meal, and the diet administered to the experimental group MWM was supplemented with 4% dried mealworm larvae meal. The nutrient digestibility of diets was determined. At the end of the experiment animals were euthanized and their digestive tracts were removed to analyze gut activity. FM group animals were characterized by lower crude fat digestibility, whereas both alternative protein sources improved the digestibility of acid detergent fiber (ADF). A considerable increase in the activity of cecal intracellular and extracellular bacterial enzymes (in particular β-glucosidase, β-galactosidase and β-xylosidase) was noted in the FM group, which however did not increase the concentrations of short-chain fatty acids (SCFA). The inclusion of MWM in chinchilla diets shifted the bacterial fermentation site from the cecum (lowest SCFA pool) to the colon (highest SCFA pool), thus enabling to derive additional energy from less digestible dietary components. In conclusion, chinchilla diets can be supplemented with small amounts of animal protein such as fish meal and dried mealworm larvae meal.","PeriodicalId":75961,"journal":{"name":"Jikken dobutsu. Experimental animals","volume":"69 1","pages":"70 - 79"},"PeriodicalIF":0.0,"publicationDate":"2019-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1538/expanim.19-0072","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47008178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sepsis is a potentially life-threatening condition, and it is frequently complicated by myocardial damage. Data on myocardial damage in rabbit caecal ligation and puncture (CLP) models are limited, although numerous animal models have been used to study sepsis-associated myocardial damage. This study aimed to investigate the effect of CLP on cardiac muscle by measuring serum cardiac troponin I (cTnI) concentrations and by detecting both histopathological changes and cTnI immunoreactivity in cardiomyocytes in rabbits. After CLP was performed in rabbits, blood samples were taken from the jugular vein at 0, 4, 8, and 12 h for haematological and biochemical analyses. At the end of the experiment, all of the rabbits were euthanised to examine the histopathological changes and the cTnI immunoreactivity in cardiac muscle tissue. No changes in serum cTnI concentration were observed in the experimental group (EG) or control group (CG) at 0 and 4 h. In EG, the mean serum cTnI concentrations were 0.230 ± 0.209 and 1.177 ± 0.971 ng/ml at 8 and 12 h, respectively. In CG, the mean serum cTnI concentrations were 0.032 ± 0.014 and 0.031 ± 0.021 ng/ml at 8 and 12 h, respectively. Moreover, cytoplasmic cTnI immunoreactivity decreased in EG compared with that in CG (P<0.01). The results demonstrated that CLP induced a systemic inflammatory response and caused myocardial damage in rabbits.
{"title":"Evaluation of cardiac troponin I in serum and myocardium of rabbits with experimentally induced polymicrobial sepsis","authors":"K. C. Tümer, H. Özdemi̇r, H. Eröksüz","doi":"10.1538/expanim.19-0046","DOIUrl":"https://doi.org/10.1538/expanim.19-0046","url":null,"abstract":"Sepsis is a potentially life-threatening condition, and it is frequently complicated by myocardial damage. Data on myocardial damage in rabbit caecal ligation and puncture (CLP) models are limited, although numerous animal models have been used to study sepsis-associated myocardial damage. This study aimed to investigate the effect of CLP on cardiac muscle by measuring serum cardiac troponin I (cTnI) concentrations and by detecting both histopathological changes and cTnI immunoreactivity in cardiomyocytes in rabbits. After CLP was performed in rabbits, blood samples were taken from the jugular vein at 0, 4, 8, and 12 h for haematological and biochemical analyses. At the end of the experiment, all of the rabbits were euthanised to examine the histopathological changes and the cTnI immunoreactivity in cardiac muscle tissue. No changes in serum cTnI concentration were observed in the experimental group (EG) or control group (CG) at 0 and 4 h. In EG, the mean serum cTnI concentrations were 0.230 ± 0.209 and 1.177 ± 0.971 ng/ml at 8 and 12 h, respectively. In CG, the mean serum cTnI concentrations were 0.032 ± 0.014 and 0.031 ± 0.021 ng/ml at 8 and 12 h, respectively. Moreover, cytoplasmic cTnI immunoreactivity decreased in EG compared with that in CG (P<0.01). The results demonstrated that CLP induced a systemic inflammatory response and caused myocardial damage in rabbits.","PeriodicalId":75961,"journal":{"name":"Jikken dobutsu. Experimental animals","volume":"69 1","pages":"54 - 61"},"PeriodicalIF":0.0,"publicationDate":"2019-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1538/expanim.19-0046","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49603963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-14DOI: 10.1538/expanim.56.103.r1
J. Iwamoto, T. Takeda, Yoshihiro Sato, J. Yeh
The following articles have been retracted by the Editorial Board of Experimental Animals, because some parts of their contents were published elsewhere.
以下文章已被《实验动物》编辑委员会撤回，因为它们的部分内容已在其他地方发表。
{"title":"Retraction: Additive effect of vitamin K2 and risedronate on long bone mass in hypophysectomized young rats","authors":"J. Iwamoto, T. Takeda, Yoshihiro Sato, J. Yeh","doi":"10.1538/expanim.56.103.r1","DOIUrl":"https://doi.org/10.1538/expanim.56.103.r1","url":null,"abstract":"The following articles have been retracted by the Editorial Board of Experimental Animals, because some parts of their contents were published elsewhere.","PeriodicalId":75961,"journal":{"name":"Jikken dobutsu. Experimental animals","volume":"68 1","pages":"r3 - r3"},"PeriodicalIF":0.0,"publicationDate":"2019-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1538/expanim.56.103.r1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43869399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-14DOI: 10.1538/expanim.55.349.r1
J. Iwamoto, A. Seki, T. Takeda, Yoshihiro Sato, Harumoto Yamada, Chwan-Li Shen, J. Yeh
The following articles have been retracted by the Editorial Board of Experimental Animals, because some parts of their contents were published elsewhere.
以下文章已被《实验动物》编辑委员会撤回，因为它们的部分内容已在其他地方发表。
{"title":"Retraction: Preventive effects of risedronate and calcitriol on cancellous osteopenia in ratstreated with high-dose glucocorticoid","authors":"J. Iwamoto, A. Seki, T. Takeda, Yoshihiro Sato, Harumoto Yamada, Chwan-Li Shen, J. Yeh","doi":"10.1538/expanim.55.349.r1","DOIUrl":"https://doi.org/10.1538/expanim.55.349.r1","url":null,"abstract":"The following articles have been retracted by the Editorial Board of Experimental Animals, because some parts of their contents were published elsewhere.","PeriodicalId":75961,"journal":{"name":"Jikken dobutsu. Experimental animals","volume":"68 1","pages":"r2 - r2"},"PeriodicalIF":0.0,"publicationDate":"2019-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43833636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-14DOI: 10.1538/expanim.53.347.r1
J. Iwamoto, T. Takeda, Yoshihiro Sato, J. Yeh
The following articles have been retracted by the Editorial Board of Experimental Animals, because some parts of their contents were published elsewhere.
以下文章已被《实验动物》编辑委员会撤回，因为它们的部分内容已在其他地方发表。
{"title":"Retraction: Response of cortical and cancellous bones to mild calcium deficiency in young growing female rats: a bone histomorphometry study","authors":"J. Iwamoto, T. Takeda, Yoshihiro Sato, J. Yeh","doi":"10.1538/expanim.53.347.r1","DOIUrl":"https://doi.org/10.1538/expanim.53.347.r1","url":null,"abstract":"The following articles have been retracted by the Editorial Board of Experimental Animals, because some parts of their contents were published elsewhere.","PeriodicalId":75961,"journal":{"name":"Jikken dobutsu. Experimental animals","volume":"68 1","pages":"r1 - r1"},"PeriodicalIF":0.0,"publicationDate":"2019-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1538/expanim.53.347.r1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48147912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bing Chen, Chongyang Qi, Li Chen, Meng-Jun Dai, Yayou Miao, Rui Chen, Wane Wei, Shun Yang, hong-Ling Wang, Xiaoge Duan, Min-Wei Gong, Yi Wang, Z. Xue
Ip3r1 encodes an inositol 1,4,5-trisphosphate-responsive calcium channel. Mutations in the IP3R1 gene in humans may cause Gillespie syndrome (GS) typically presents as fixed dilated pupils in affected infants, which was referred to as iris hypoplasia. However, there is no report of mice with Ip3r1 heterozygous mutations showing dilated pupils. Here, we report a new Ip3r1 allele with short-term dilated pupil phenotype derived from an N-ethyl-N-nitrosourea (ENU) mutagenesis screen. This allele carries a G5927A transition mutation in Ip3r1 gene (NM_010585), which is predicted to result in a C1976Y amino acid change in the open reading frame of IP3R1 (NP_034715). We named this novel Ip3r1 allele Ip3r1C1976Y. Histology and pharmacological tests show that the dilated pupil phenotype is a mydriasis caused by the functional defect in the iris constrictor muscles in Ip3r1C1976Y. The dilated pupil phenotype in Ip3r1C1976Y was referred to as mydriasis and excluding iris hypoplasia. IHC analysis revealed increased expression of BIP protein, the master regulator of unfolded protein response (UPR) signaling, in Ip3r1C1976Y mice that did not recover. This study is the first report of an Ip3r1 mutation being associated with the mydriasis phenotype. Ip3r1C1976Y mice represent a self-healing model that may be used to study the therapeutic approach for Ip3r1-related diseases.
{"title":"A C1976Y missense mutation in the mouse Ip3r1 gene leads to short-term mydriasis and unfolded protein response in the iris constrictor muscles","authors":"Bing Chen, Chongyang Qi, Li Chen, Meng-Jun Dai, Yayou Miao, Rui Chen, Wane Wei, Shun Yang, hong-Ling Wang, Xiaoge Duan, Min-Wei Gong, Yi Wang, Z. Xue","doi":"10.1538/expanim.19-0007","DOIUrl":"https://doi.org/10.1538/expanim.19-0007","url":null,"abstract":"Ip3r1 encodes an inositol 1,4,5-trisphosphate-responsive calcium channel. Mutations in the IP3R1 gene in humans may cause Gillespie syndrome (GS) typically presents as fixed dilated pupils in affected infants, which was referred to as iris hypoplasia. However, there is no report of mice with Ip3r1 heterozygous mutations showing dilated pupils. Here, we report a new Ip3r1 allele with short-term dilated pupil phenotype derived from an N-ethyl-N-nitrosourea (ENU) mutagenesis screen. This allele carries a G5927A transition mutation in Ip3r1 gene (NM_010585), which is predicted to result in a C1976Y amino acid change in the open reading frame of IP3R1 (NP_034715). We named this novel Ip3r1 allele Ip3r1C1976Y. Histology and pharmacological tests show that the dilated pupil phenotype is a mydriasis caused by the functional defect in the iris constrictor muscles in Ip3r1C1976Y. The dilated pupil phenotype in Ip3r1C1976Y was referred to as mydriasis and excluding iris hypoplasia. IHC analysis revealed increased expression of BIP protein, the master regulator of unfolded protein response (UPR) signaling, in Ip3r1C1976Y mice that did not recover. This study is the first report of an Ip3r1 mutation being associated with the mydriasis phenotype. Ip3r1C1976Y mice represent a self-healing model that may be used to study the therapeutic approach for Ip3r1-related diseases.","PeriodicalId":75961,"journal":{"name":"Jikken dobutsu. Experimental animals","volume":"69 1","pages":"45 - 53"},"PeriodicalIF":0.0,"publicationDate":"2019-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1538/expanim.19-0007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45800299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-31DOI: 10.1538/expanim.68suppl-LAS2
Shinya Ayabe, Masayo Kadota, Tomomi Hashimoto, Kenichi Nakashima, A. Yoshiki
Genome Editing technologies enable us to produce the Knock-out mouse within a short period of time. Opportunity for the novice to produce the Knock-out mouse is increased. In many cases, in-del mutations are induced in coding sequence of the target genes for its Knock-out. Although in-del mutation could be induced with high efficiency, there is a risk of incomplete Knock-out. If the methionine coding ATG located downstream of mutation site function as start codon, in-del induced gene still has potential for expressing the functional protein in which just a few amino-acid at N-terminal defected. This undesired event is called illegitimate translation (ITL). To avoid ITL, we adopt Excision Knock-out. When double strand break happen on the two sites locating up and downstream of the full-length target gene region, this region can be excised. Even though the rate of this excision mutation is lower than that of in-del mutation, there is no risk of ITL. However, Full-length gene excision has another risk; promoter/enhancer regions of the neighboring genes and the non-coding genes are also deleted. Therefore, we recommend to excise the Critical Exon when your target gene is large. It requires knowledge of genetics for selecting Critical Exon. To overcome this problem, we develop new CRISPR design tool, KOnezumi. KOnezumi shows not only Critical Exon but also sgRNA sequence and primer sites when you just input your target gene name. The 66th Annual Meeting of Japanese Association for Laboratory Animal Science
{"title":"LAS Seminar 2","authors":"Shinya Ayabe, Masayo Kadota, Tomomi Hashimoto, Kenichi Nakashima, A. Yoshiki","doi":"10.1538/expanim.68suppl-LAS2","DOIUrl":"https://doi.org/10.1538/expanim.68suppl-LAS2","url":null,"abstract":"Genome Editing technologies enable us to produce the Knock-out mouse within a short period of time. Opportunity for the novice to produce the Knock-out mouse is increased. In many cases, in-del mutations are induced in coding sequence of the target genes for its Knock-out. Although in-del mutation could be induced with high efficiency, there is a risk of incomplete Knock-out. If the methionine coding ATG located downstream of mutation site function as start codon, in-del induced gene still has potential for expressing the functional protein in which just a few amino-acid at N-terminal defected. This undesired event is called illegitimate translation (ITL). To avoid ITL, we adopt Excision Knock-out. When double strand break happen on the two sites locating up and downstream of the full-length target gene region, this region can be excised. Even though the rate of this excision mutation is lower than that of in-del mutation, there is no risk of ITL. However, Full-length gene excision has another risk; promoter/enhancer regions of the neighboring genes and the non-coding genes are also deleted. Therefore, we recommend to excise the Critical Exon when your target gene is large. It requires knowledge of genetics for selecting Critical Exon. To overcome this problem, we develop new CRISPR design tool, KOnezumi. KOnezumi shows not only Critical Exon but also sgRNA sequence and primer sites when you just input your target gene name. The 66th Annual Meeting of Japanese Association for Laboratory Animal Science","PeriodicalId":75961,"journal":{"name":"Jikken dobutsu. Experimental animals","volume":"68 1","pages":"S36 - S39"},"PeriodicalIF":0.0,"publicationDate":"2019-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41637851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-31DOI: 10.1538/expanim.68suppl-S2
○Kathryn Bayne, ○Yoshie Kakuma
One Health is defined by the One Health Commission as “... a collaborative ... and trans-disciplinary approach, working locally, regionally, nationally, and globally, to achieve optimal health and well-being of all animals, people, plants and their shared environment, recognizing their inextricable interconnections.” The concept that the health of humans, animals and the environment are linked is not new, with similarities between animals and humans noted by Aristotle. However, according to the CDC, human and animal medicine were considered separate disciplines until the 20th century. Only in the last few years has it been recognized that the welfare of humans, animals and the environment are also connected, with the resulting use of the term, One Welfare. Examples of the linkage of these include: animal abuse is often linked to human abuse; improving animal welfare can improve urban social indicators; improved animal welfare enhances food safety; improved animal welfare increases farm productivity; improved wildlife welfare enhances biodiversity conservation; and improved research animal welfare enhances data reproducibility and validity. The One Welfare concept fosters the United Nations’ Sustainability Development Goals (UN SDGs) by building “economic growth and address[ing] a range of social needs including education, health, social protection, and job opportunities....” In this way, the One Welfare concept is connected to the Tokyo Declaration on Universal Health Coverage, which also supports the UN SDGs, and thus is of interest to the Japanese people. This presentation will describe One Welfare, including its place in biomedical research. The 66th Annual Meeting of Japanese Association for Laboratory Animal Science
{"title":"Symposium 2","authors":"○Kathryn Bayne, ○Yoshie Kakuma","doi":"10.1538/expanim.68suppl-S2","DOIUrl":"https://doi.org/10.1538/expanim.68suppl-S2","url":null,"abstract":"One Health is defined by the One Health Commission as “... a collaborative ... and trans-disciplinary approach, working locally, regionally, nationally, and globally, to achieve optimal health and well-being of all animals, people, plants and their shared environment, recognizing their inextricable interconnections.” The concept that the health of humans, animals and the environment are linked is not new, with similarities between animals and humans noted by Aristotle. However, according to the CDC, human and animal medicine were considered separate disciplines until the 20th century. Only in the last few years has it been recognized that the welfare of humans, animals and the environment are also connected, with the resulting use of the term, One Welfare. Examples of the linkage of these include: animal abuse is often linked to human abuse; improving animal welfare can improve urban social indicators; improved animal welfare enhances food safety; improved animal welfare increases farm productivity; improved wildlife welfare enhances biodiversity conservation; and improved research animal welfare enhances data reproducibility and validity. The One Welfare concept fosters the United Nations’ Sustainability Development Goals (UN SDGs) by building “economic growth and address[ing] a range of social needs including education, health, social protection, and job opportunities....” In this way, the One Welfare concept is connected to the Tokyo Declaration on Universal Health Coverage, which also supports the UN SDGs, and thus is of interest to the Japanese people. This presentation will describe One Welfare, including its place in biomedical research. The 66th Annual Meeting of Japanese Association for Laboratory Animal Science","PeriodicalId":75961,"journal":{"name":"Jikken dobutsu. Experimental animals","volume":"68 1","pages":"S16 - S18"},"PeriodicalIF":0.0,"publicationDate":"2019-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49285198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-31DOI: 10.1538/expanim.68suppl-LAS3
A. Yoshiki
New mouse users are increasing with the spread of genome editing. This seminar is aiming to introduce how to use fully domestic and overseas mouse resource organizations and related infrastructures where various mouse strains so far developed have been preserved. The speaker will explain paper works to obtain mice from major mouse resource organizations and quality issues to be considered when receiving the mice. The introductory talk will encompass the one-stop shop database that can list all strains stored in major resource organizations around the world (International Mouse Strain Resource: IMSR), and the international mouse phenotyping consortium (IMPC) that is establishing knockout mouse strains of all protein-coding genes and the phenotype information. The author is expecting to share with participants the knowledge of useful research infrastructures available for full use of mouse resources, including breeder companies which provide standard strains and technical services. The 66th Annual Meeting of Japanese Association for Laboratory Animal Science
{"title":"LAS Seminar 3","authors":"A. Yoshiki","doi":"10.1538/expanim.68suppl-LAS3","DOIUrl":"https://doi.org/10.1538/expanim.68suppl-LAS3","url":null,"abstract":"New mouse users are increasing with the spread of genome editing. This seminar is aiming to introduce how to use fully domestic and overseas mouse resource organizations and related infrastructures where various mouse strains so far developed have been preserved. The speaker will explain paper works to obtain mice from major mouse resource organizations and quality issues to be considered when receiving the mice. The introductory talk will encompass the one-stop shop database that can list all strains stored in major resource organizations around the world (International Mouse Strain Resource: IMSR), and the international mouse phenotyping consortium (IMPC) that is establishing knockout mouse strains of all protein-coding genes and the phenotype information. The author is expecting to share with participants the knowledge of useful research infrastructures available for full use of mouse resources, including breeder companies which provide standard strains and technical services. The 66th Annual Meeting of Japanese Association for Laboratory Animal Science","PeriodicalId":75961,"journal":{"name":"Jikken dobutsu. Experimental animals","volume":"68 1","pages":"S40 - S43"},"PeriodicalIF":0.0,"publicationDate":"2019-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41547220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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