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A Rapid Laser Probing Method Facilitates the Non-invasive and Contact-free Determination of Leaf Thermal Properties. 快速激光探测法有助于非侵入式和非接触式测定叶片热特性。
IF 1.2 Pub Date : 2017-01-07 DOI: 10.3791/54835
Johannes F Buyel, Hannah M Gruchow, Martin Wehner

Plants can produce valuable substances such as secondary metabolites and recombinant proteins. The purification of the latter from plant biomass can be streamlined by heat treatment (blanching). A blanching apparatus can be designed more precisely if the thermal properties of the leaves are known in detail, i.e., the specific heat capacity and thermal conductivity. The measurement of these properties is time consuming and labor intensive, and usually requires invasive methods that contact the sample directly. This can reduce the product yield and may be incompatible with containment requirements, e.g., in the context of good manufacturing practice. To address these issues, a non-invasive, contact-free method was developed that determines the specific heat capacity and thermal conductivity of an intact plant leaf in about one minute. The method involves the application of a short laser pulse of defined length and intensity to a small area of the leaf sample, causing a temperature increase that is measured using a near infrared sensor. The temperature increase is combined with known leaf properties (thickness and density) to determine the specific heat capacity. The thermal conductivity is then calculated based on the profile of the subsequent temperature decline, taking thermal radiation and convective heat transfer into account. The associated calculations and critical aspects of sample handling are discussed.

植物可以产生有价值的物质,如次级代谢产物和重组蛋白质。通过热处理(焯水)可以简化从植物生物质中提纯后者的过程。如果能详细了解叶片的热特性,即比热容和热传导率,就能更精确地设计焯水设备。这些特性的测量耗时耗力,通常需要采用直接接触样品的侵入式方法。这可能会降低产品产量,而且可能不符合封闭性要求,例如在良好生产规范的背景下。为解决这些问题,我们开发了一种非侵入性、非接触式方法,可在一分钟内测定完整植物叶片的比热容和导热率。该方法是在叶片样本的一小块区域上施加一个规定长度和强度的短激光脉冲,导致温度上升,然后使用近红外传感器测量温度。温度升高与已知的叶片特性(厚度和密度)相结合,确定比热容。然后根据随后的温度下降曲线计算热导率,同时考虑热辐射和对流传热。讨论了相关计算和样品处理的关键问题。
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引用次数: 0
Effects of a metronome on the filled pauses of fluent speakers. 节拍器对流利说话者停顿的影响。
Pub Date : 1996-12-01 DOI: 10.1044/jshr.3906.1232
N Christenfeld

Filled pauses (the "ums" and "uhs" that litter spontaneous speech) seem to be a product of the speaker paying deliberate attention to the normally automatic act of talking. This is the same sort of explanation that has been offered for stuttering. In this paper we explore whether a manipulation that has long been known to decrease stuttering, synchronizing speech to the beats of a metronome, will then also decrease filled pauses. Two experiments indicate that a metronome has a dramatic effect on the production of filled pauses. This effect is not due to any simplification or slowing of the speech and supports the view that a metronome causes speakers to attend more to how they are talking and less to what they are saying. It also lends support to the connection between stutters and filled pauses.

充满停顿(“嗯”和“啊”,打乱了自发的讲话)似乎是说话者刻意注意正常自动说话行为的产物。这和对口吃的解释是一样的。在本文中,我们探讨了一种长期以来被认为可以减少口吃的操作,将语音与节拍器的节拍同步,然后也会减少填充停顿。两个实验表明节拍器对填充停顿的产生有显著的影响。这种效果并不是由于讲话的任何简化或放慢,而是支持节拍器使说话者更多地关注他们说话的方式而不是他们所说的内容的观点。它还为结巴和停顿之间的联系提供了支持。
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引用次数: 22
Toward tense as a clinical marker of specific language impairment in English-speaking children. 趋向时态作为英语儿童特异性语言障碍的临床标志。
Pub Date : 1996-12-01 DOI: 10.1044/jshr.3906.1239
M L Rice, K Wexler

A critical clinical issue is the identification of a clinical marker, a linguistic form or principle that can be shown to be characteristic of children with Specific Language Impairment (SLI). In this paper we evaluate, as candidate clinical markers, a set of morphemes that mark Tense. In English, this includes -s third person singular, -ed regular past, BE, and DO. According to the Extended Optional Infinitive Account (EOI) of Rice, Wexler, and Cleave (1995), this set of morphemes is likely to appear optionally in the grammars of children with SLI at a rate lower than the optionality evident in younger controls. Three groups of preschool children participated: 37 children with SLI, and two control groups, one of 40 MLU-equivalent children and another of 45 age-equivalent children. Three kinds of evidence support the conclusion that a set of morphemes that marks Tense can be considered a clinical marker: (a) low levels of accuracy for the target morphemes for the SLI group relative to either of the two control groups; (b) affectedness for the set of morphemes defined by the linguistic function of Tense, but not for morphemes unrelated to Tense; and (c) a bimodal distribution for Tense-marking morphemes relative to age peers, in which the typical children are at essentially adult levels of the grammar, whereas children in the SLI group were at low (i.e., non-adultlike) levels of performance. The clinical symptoms are evident in omissions of surface forms. Errors of subject-verb agreement and syntactic misuses are rare, showing that, as predicted, children in an EOI stage who are likely to mark Tense optionally at the same time know a great deal about the grammatical properties of finiteness and agreement in the adult grammar. The findings are discussed in terms of alternative accounts of the grammatical limitations of children with SLI and implications for clinical identification.

一个关键的临床问题是确定一个临床标志,一种语言形式或原则,可以显示出特殊语言障碍(SLI)儿童的特征。在本文中,我们评估了一组标记Tense的语素作为候选临床标志物。在英语中,这包括-s第三人称单数,-ed一般过去时,BE和DO。根据Rice, Wexler和Cleave(1995)的扩展可选不定式描述(EOI),这组语素可能会选择性地出现在特殊语言障碍儿童的语法中,其出现率低于年轻对照组的可选性。三组学龄前儿童参与:37名患有特殊语言障碍的儿童和两个对照组,其中一组有40名与mlu相当的儿童,另一组有45名与年龄相当的儿童。有三种证据支持这一结论,即一组标记时态的语素可以被认为是临床标记:(a)相对于两个对照组中的任何一个,特殊语言障碍组的目标语素的准确性水平较低;(b)对由时态的语言功能定义的语素集的影响,但对与时态无关的语素没有影响;(c)时态标记语素相对于同龄人的双峰分布,其中典型儿童的语法基本上处于成人水平,而特殊语言障碍组的儿童则处于较低(即非成人)的表现水平。临床症状是明显的遗漏表面形式。主谓一致和句法误用的错误很少,这表明,正如预期的那样,处于情感表达阶段的儿童可能会选择性地标记时态,同时对成人语法中的有限性和一致性的语法特性有很大的了解。研究结果讨论了对特殊语言障碍儿童的语法限制的替代解释和临床鉴定的意义。
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引用次数: 919
Tactile perception of spatial stimuli on the lip surface by young and older adults. 年轻人和老年人对唇表面空间刺激的触觉感知。
Pub Date : 1996-12-01 DOI: 10.1044/jshr.3906.1191
A B Wohlert

Although older adults are subject to both subtle changes and major disorders of the oral sensorimotor system, relatively little is known about oral sensory function in old age. Accurate assessment of oral tactile perception is needed to document disability, aid prognosis, and plan treatment for older adults with disorders affecting speech or feeding. However, normative information currently available for older adults is mainly based on two-point discrimination, a problematic measure of tactile spatial resolution. Grating orientation discrimination, a technique developed to provide a clear and reliable measure of spatial resolution, was used to test sensitivity of the upper and lower lip vermillion, on right and left sides, in a sample of 40 young adults and 40 adults age 66-85. Results indicated that spatial acuity at the lip vermilion declines significantly in old age and that women tend to have better acuity than men. No significant differences were found in acuity between the upper and lower lips or between right and left sides for either age group. Peripheral changes in receptor density and lip tissue composition are suggested as likely causes for the age-related decline.

尽管老年人易受口腔感觉运动系统细微变化和重大疾病的影响,但对老年人口腔感觉功能的了解相对较少。对于影响言语或进食障碍的老年人,需要准确的口腔触觉感知评估来记录残疾、帮助预后和计划治疗。然而,目前对老年人可用的规范性信息主要基于两点辨别,这是一种有问题的触觉空间分辨率测量。光栅定向辨别技术是一种为提供清晰可靠的空间分辨率测量而开发的技术,该技术被用于测试上下唇红粉在左右两侧的灵敏度,样本包括40名年轻人和40名年龄在66-85岁之间的成年人。结果表明,随着年龄的增长,红唇的空间敏锐度明显下降,女性的敏锐度往往优于男性。两组患者的上下唇或左右唇的敏锐度无显著差异。外周受体密度和嘴唇组织组成的变化被认为是与年龄相关的衰退的可能原因。
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引用次数: 56
Consonant confusions in amplitude-expanded speech. 扩音语音中的辅音混淆。
Pub Date : 1996-12-01 DOI: 10.1044/jshr.3906.1124
R L Freyman, G P Nerbonne

The perceptual consequences of expanding the amplitude variations in speech were studied under conditions in which spectral information was obscured by signal correlated noise that had an envelope correlated with the speech envelope, but had a flat amplitude spectrum. The noise samples, created individually from 22 vowel-consonant-vowel nonsense words, were used as maskers of those words, with signal-to-noise ratios ranging from -15 to 0 dB. Amplitude expansion was by a factor of 3.0 in terms of decibels. In the first experiment, presentation level for speech peaks was 80 dB SPL. Consonant recognition performance for expanded speech by 50 listeners with normal hearing was as much as 30 percentage points poorer than for unexpanded speech and the types of errors were dramatically different, especially in the midrange of S-N ratios. In a second experiment presentation level was varied to determine whether reductions in consonant levels produced by expansion were responsible for the differences between conditions. Recognition performance for unexpanded speech at 40 dB SPL was nearly equivalent to that for expanded speech at 80 dB SPL. The error patterns obtained in these two conditions were different, suggesting that the differences between conditions in Experiment 1 were due largely to expanded amplitude envelopes rather than differences in audibility.

在频谱信息被信号相关噪声掩盖的条件下,扩大语音振幅变化的感知后果进行了研究,这些噪声的包络与语音包络相关,但具有平坦的幅度谱。噪声样本是由22个元音-辅音-元音的无意义单词单独产生的,它们被用作这些单词的掩码,信噪比从-15分贝到0分贝不等。振幅的扩大是分贝的3倍。在第一个实验中,语音峰值的呈现水平为80 dB SPL。50名听力正常的听者在扩展语音时的辅音识别表现比未扩展语音差30个百分点,错误类型也有显著差异,特别是在S-N比的中间区间。在第二个实验中,呈现水平是不同的,以确定是否由扩张产生的辅音水平的减少是造成条件差异的原因。40 dB声压级下未扩展语音的识别性能与80 dB声压级下扩展语音的识别性能基本相当。两种情况下的误差模式不同,表明实验1中两种情况的差异主要是由于振幅包络的扩大,而不是可听性的差异。
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引用次数: 11
Sample size effects on temporal reliability of language sample measures of preschool children. 样本大小对学龄前儿童语言样本测量时间信度的影响。
Pub Date : 1996-12-01 DOI: 10.1044/jshr.3906.1258
W J Gavin, L Giles

The present study examined the temporal reliability of four quantitative measurements of operationally defined linguistic behaviors observed in a naturalistic setting. The measures of language production were computed using the Systematic Analysis of Language Transcripts (SALT) software from two 20-minute language samples obtained 3 to 14 days apart for each of 20 preschool-aged children. Samples were edited to different sizes based either on duration (12 or 20 minutes) or on limits of the total number of complete and intelligible utterances (from 25 to 175 in increments of 25). Inadequate reliability was found for the language sample measure, total number of words; hence, the validity of this measure is questionable. In contrast, very high temporal reliability coefficients (r > .92) were obtained for the language sample measures of number of different words, mean length of utterance in morphemes, and mean sentence length in morphemes when derived from a large number (> or = 175) of complete and intelligible utterances. The temporal reliability of these measures reached acceptable levels, not only for research purposes, but for diagnostic purposes as well.

本研究考察了在自然环境中观察到的操作性定义语言行为的四种定量测量的时间可靠性。使用语言转录本系统分析(SALT)软件对20名学龄前儿童的两个20分钟语言样本进行计算,每个样本间隔3至14天。根据持续时间(12分钟或20分钟)或完整和可理解的话语总数的限制(从25到175,增量为25),将样本编辑成不同的大小。语言样本测量词总数的信度不足;因此,这一措施的有效性值得怀疑。相比之下,当从大量(>或= 175)完整可理解的话语中提取时,不同单词的数量、语素的平均话语长度和语素的平均句子长度的语言样本测量获得了非常高的时间信度系数(r > .92)。这些测量的时间可靠性达到了可接受的水平,不仅用于研究目的,而且用于诊断目的。
{"title":"Sample size effects on temporal reliability of language sample measures of preschool children.","authors":"W J Gavin,&nbsp;L Giles","doi":"10.1044/jshr.3906.1258","DOIUrl":"https://doi.org/10.1044/jshr.3906.1258","url":null,"abstract":"<p><p>The present study examined the temporal reliability of four quantitative measurements of operationally defined linguistic behaviors observed in a naturalistic setting. The measures of language production were computed using the Systematic Analysis of Language Transcripts (SALT) software from two 20-minute language samples obtained 3 to 14 days apart for each of 20 preschool-aged children. Samples were edited to different sizes based either on duration (12 or 20 minutes) or on limits of the total number of complete and intelligible utterances (from 25 to 175 in increments of 25). Inadequate reliability was found for the language sample measure, total number of words; hence, the validity of this measure is questionable. In contrast, very high temporal reliability coefficients (r > .92) were obtained for the language sample measures of number of different words, mean length of utterance in morphemes, and mean sentence length in morphemes when derived from a large number (> or = 175) of complete and intelligible utterances. The temporal reliability of these measures reached acceptable levels, not only for research purposes, but for diagnostic purposes as well.</p>","PeriodicalId":76022,"journal":{"name":"Journal of speech and hearing research","volume":"39 6","pages":"1258-62"},"PeriodicalIF":0.0,"publicationDate":"1996-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1044/jshr.3906.1258","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19923562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 94
Aerodynamic and temporal aspects of velopharyngeal function in normal speakers. 正常说话者腭咽功能的气动和时间方面。
Pub Date : 1996-12-01 DOI: 10.1044/jshr.3906.1199
D J Zajac, R Mayo

Aerodynamic and temporal characteristics of velopharyngeal function were determined for 42 adult male and female speakers. All subjects produced the word "hamper" at self-determined loudness levels and rates of speaking. Measurements of intraoral air pressure, nasal airflow, and estimates of velopharyngeal orifice size were obtained during production of the /m/ and /p/ segments. Volume measurements of nasal airflow were determined for the entire word, the /m/ segment, and the segments preceding /m/. Fifteen timing measures associated with the pressure-flow events of the nasal-plosive sequence were also determined. Results indicated that males generated significantly higher levels of peak intraoral air pressure than females during /p/. Male speakers also exhibited a significantly shorter interval in the rise of oral pressure associated with the /p/ segment. Male and female speakers, however, exhibited similar levels of anticipatory coarticulation as reflected by nasal air volume measurements. Finally, variability of selected measurements within speakers suggested that temporal aspects of velopharyngeal function were more constrained than aerodynamic aspects. The results are discussed relative to (a) sex differences in respiratory and velar function and (b) normative data for adult speakers.

本文测定了42名成年男女说话者的腭咽功能的气动特征和时间特征。所有的研究对象都以自己决定的音量和语速说出“妨碍”这个词。在制作/m/和/p/段期间,测量口内气压、鼻腔气流和估计腭咽口尺寸。测量整个单词、/m/音段和/m/音段之前的鼻腔气流量。还确定了与鼻爆序列的压力-流量事件相关的15个定时措施。结果表明,在/p/期间,男性产生的口内气压峰值水平明显高于女性。男性说话者在发/p/音时,口腔压力的上升间隔也明显缩短。然而,男性和女性说话者表现出相似的预期协同发音水平,这反映在鼻腔空气量的测量中。最后,扬声器内选择测量的可变性表明,腭咽功能的时间方面比空气动力学方面更受限制。结果讨论相对于(a)呼吸和腭功能的性别差异和(b)成人说话者的规范数据。
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引用次数: 59
Functional-lesion investigation of developmental stuttering with positron emission tomography. 发展性口吃功能损害的正电子发射断层研究。
Pub Date : 1996-12-01 DOI: 10.1044/jshr.3906.1208
R J Ingham, P T Fox, J C Ingham, F Zamarripa, C Martin, P Jerabek, J Cotton

Positron emission tomographic (PET) H2(15)O measurements of resting-state regional cerebral blood flow (CBF) were obtained in 29 right-handed men, 10 of whom stuttered and 19 of whom did not. PET images were analyzed by sampling 74 regions of interest (ROIs), 37 per hemisphere. ROI placement was guided both physiologically and anatomically. Physiological ROI placement was based on speech motor activations. Anatomical ROIs were positioned by reference to a stereotactic, neurosurgical atlas with positions confirmed and finely adjusted by co-registered magnetic-resonance images (MRIs). For all subjects, PET and MR images were normal to visual inspection. Highly significant (p < 0.0001) between-region and between-hemisphere effects were found for both groups, as have been previously reported for normal subjects, but no significant between-group differences were found for any regional CBF values. Analysis by a laterality index found a weakly significant between-groups effect (p = 0.04) that was isolated to five regions, four of which are implicated in speech or hearing. However, these regional laterality effects showed no consistent directionality, nor did these regions have absolute differences in regional blood flow between groups. Present findings do not support recent suggestions that developmental stuttering is associated with abnormalities of brain blood flow at rest. Rather, our findings indicate an essentially normal functional brain terrain with a small number of minor differences in hemispheric symmetry.

对29名右撇子男性进行静息状态区域脑血流量(CBF)的正电子发射断层扫描(PET) H2(15)O测量,其中10名口吃,19名非口吃。PET图像通过采样74个感兴趣区域(roi)进行分析,每个半球37个。从生理学和解剖学角度指导ROI的放置。生理ROI定位是基于言语运动激活。解剖roi通过参考立体定向、神经外科图谱进行定位,并通过共同注册的磁共振图像(mri)确定和精细调整位置。所有受试者的PET和MR图像视觉检查正常。与之前报道的正常受试者一样,在两组中都发现了非常显著的区域间和半球间效应(p < 0.0001),但在任何区域CBF值方面没有发现显著的组间差异。通过侧偏指数分析发现,组间效应弱显著(p = 0.04),被隔离到五个区域,其中四个涉及语言或听力。然而,这些局部偏侧效应并没有显示出一致的方向性,这些区域在两组之间的区域血流也没有绝对差异。目前的研究结果不支持最近提出的发展性口吃与休息时脑血流异常有关的建议。相反,我们的研究结果表明,在半球对称性方面存在少量细微差异的基本正常的脑功能地形。
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引用次数: 59
Microphone sensitivity as a source of variation in nasalance scores. 麦克风灵敏度作为鼻音平衡分数变化的来源。
Pub Date : 1996-12-01 DOI: 10.1044/jshr.3906.1228
D J Zajac, R Lutz, R Mayo

A two-part study was conducted to determine the sources of variation in nasalance scores derived from the Nasometer. In Study #1, a function generator was used as a signal source to calibrate and input sine and square waves directly into the Nasometer. Ten stimuli ranging from 105 to 330 Hz in 25 Hz increments were evaluated. In Study #2, the same signal source and an amplified loudspeaker were used to calibrate and present square waves to the nasometer via five different sets of microphones. The sound pressure level of all stimuli was maintained at 88 dB. Each microphone set was calibrated using the 105 Hz signals. Results from Study #1 indicated consistent nasalance scores across all frequencies (i.e., all scores were within 2% of calibration). Results from Study #2 demonstrated deviations greater than 2% from calibration as a function of frequency for all five sets of microphones. The smallest deviation was 5%, whereas the largest deviation was 14%. We suggest that the variation in nasalance as a function of stimulus frequency may be due to a mismatch in the sensitivity of microphones (i.e., different frequency response characteristics). It is further suggested (a) that individual investigators determine the response characteristics of their microphones and (b) that relatively small variations in nasalance scores (i.e., 5-14%) either within or across speakers be interpreted with caution.

一项由两部分组成的研究被用于确定由鼻量计得出的鼻平衡评分变异的来源。在研究#1中,使用函数发生器作为信号源来校准正弦波和方波,并将其直接输入到Nasometer中。以25 Hz的增量评估10个105 - 330 Hz的刺激。在研究#2中,使用相同的信号源和一个放大扬声器来校准,并通过五组不同的麦克风将方波呈现给鼻计。所有刺激声压级均维持在88 dB。每个麦克风组使用105 Hz信号进行校准。研究1的结果表明,所有频率的鼻平衡评分一致(即,所有评分都在校准的2%以内)。研究#2的结果表明,所有五组麦克风的校准偏差都大于2%,作为频率的函数。最小偏差为5%,最大偏差为14%。我们认为,鼻平衡的变化作为刺激频率的函数可能是由于麦克风灵敏度的不匹配(即不同的频率响应特性)。进一步建议(a)个别调查人员确定其麦克风的响应特性,(b)在扬声器内部或扬声器之间的相对较小的鼻平衡分数变化(即5-14%)应谨慎解释。
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引用次数: 36
Auditory lexical decisions of children with specific language impairment. 特殊语言障碍儿童的听觉词汇决策。
Pub Date : 1996-12-01 DOI: 10.1044/jshr.3906.1263
J Edwards, M Lahey

To determine whether children with specific language impairment (SLI) take longer than age peers to recognize sequences of sounds that represent words in their lexicon, we compared auditory lexical decision times of children with SLI to those of typically developing age peers. Children with SLI were significantly slower than peers, but speed of word recognition was not correlated with measures of language comprehension for children with SLI. Furthermore, time to detect an auditory signal and initiate a vocal response did not account for the differences between groups. Possible interpretations of the results are discussed with two explanations-differences between groups in task-related factors that stressed processing capacity or in the nature of phonetic/phonological representations-seeming more likely than others.

为了确定患有特殊语言障碍(SLI)的儿童是否比同龄儿童需要更长的时间来识别他们词汇中代表单词的声音序列,我们比较了患有特殊语言障碍(SLI)的儿童与正常发展的同龄儿童的听觉词汇决策时间。患有特殊语言障碍的儿童的语言理解速度明显比同龄人慢,但单词识别速度与语言理解能力没有相关性。此外,检测听觉信号和发起声音反应的时间并不能解释两组之间的差异。对研究结果的可能解释有两种解释,一种是组间在任务相关因素上的差异,这些因素强调处理能力,另一种是在语音/语音表征的本质上的差异,这两种解释似乎比其他解释更有可能。
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引用次数: 98
期刊
Journal of speech and hearing research
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