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Evaluation of Ugandan Cowpea Germplasm for Yield and Resistance to Scab Disease 乌干达豇豆种质产量及抗赤霉病评价
Pub Date : 2016-01-10 DOI: 10.9734/AJEA/2016/25138
E. Afutu, K. E. Mohammed, T. Odong, M. Biruma, P. Rubaihayo
Aims: This study was conducted to identify cowpea (Vigna unguiculata) cultivars with high yield potential and resistance to scab disease caused by Sphaceloma sp. in Uganda. 100 cowpea genotypes were evaluated at two locations (Kabanyolo and Serere) in Uganda between April and July, 2014 using a 10 × 10 alpha lattice design. Place and Duration of Study: Makerere University Agriculture Research Institute Kabanyolo (MUARIK) and the National Semi Arid Resources Research Institute (NaSARRI) – Serere between April to July, 2014. Study Design and Methodology: Hundred cowpea lines (69 landraces, 25 inbred lines at F7, 1 local and 5 improved cultivars) were grown at each location in a 10 × 10 alpha lattice design with 3 replications to assess their reaction to the scab disease and yield potential (grain yield and yield related traits). Results: The cowpea lines differed significantly (P = .05) in their response to natural disease Original Research Article Afutu et al.; AJEA, 12(2): 1-18, 2016; Article no.AJEA.25138 2 pressure as determined by disease incidence, apparent infection rate (r) and severity indicated by area under disease progress curve (AUDPC). Analysis of variance showed that there was highly significant differences (P < .001) in genotypes, locations, AUDPC and other traits and genotype by location (G×L) interaction on AUDPC. The correlation analysis revealed a positive relationship of scab disease incidence with AUDPC (0.8; P < .001) but a significant (P < .001) negative relationship with grain yield (-0.8), number of pods per plant (-0.5), number of seeds per pod (-0.5) and 100 seed weight (-0.5). Cluster analysis based on only scab disease indexes produced 4 main clusters while cluster analysis based on disease and yield traits produced 3 main clusters. However, the two different clusters revealed similar grouping patterns in which cowpea lines with similar resistance ratings were shown to form unique clusters. R-mode principal component analysis yielded 4 principal components explaining 62.28% of the variation observed. Conclusion: The study revealed that the use of apparent infection rate “r” alone as an index for rating a genotype for scab reaction was not decisive. One line (NE 15) was found to be resistant to the scab disease at both locations and high yielding and could be used in the cowpea improvement programme to breed for resistance to the scab disease.
目的:利用10 × 10 α格设计,于2014年4 - 7月在乌干达两个地点(Kabanyolo和Serere)对100个豇豆基因型进行了鉴定,以鉴定具有高产潜力和抗Sphaceloma sp.痂病的豇豆(Vigna unguiculata)品种。研究地点和时间:Makerere大学农业研究所Kabanyolo (MUARIK)和国家半干旱资源研究所(NaSARRI) - 2014年4月至7月。研究设计和方法:采用10 × 10 α晶格设计,在每个地点种植100个豇豆品系(69个地方品系,25个F7自交系,1个地方品系和5个改良品系),3个重复,评估它们对赤霉病的反应和产量潜力(籽粒产量和产量相关性状)。结果:豇豆品系对自然病害的反应差异有统计学意义(P = 0.05)。农业工程学报,12(2):1-18,2016;文章no.AJEA。以疾病发病率、表观感染率(r)和疾病进展曲线下面积(AUDPC)表示的严重程度来确定压力。方差分析显示,基因型、位点、AUDPC等性状和基因型与位点(G×L)互作在AUDPC上存在极显著差异(P < 0.001)。相关分析显示结痂病发生率与AUDPC呈正相关(0.8;P < 0.001),但与籽粒产量(-0.8)、单株荚果数(-0.5)、单株荚果数(-0.5)和百粒重(-0.5)呈显著负相关(P < 0.001)。仅基于病害指标的聚类分析得到4个主要聚类,而基于病害性状和产量性状的聚类分析得到3个主要聚类。然而,两个不同的簇显示出相似的分组模式,具有相似抗性等级的豇豆株系形成独特的簇。r型主成分分析得到4个主成分,解释了62.28%的变异。结论:单纯以表观感染率“r”作为评价结痂反应基因型的指标不具有决定性。其中一个品系(ne15)在两个地点均具有抗痂病性和高产性,可用于豇豆抗痂病育种的改良计划。
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引用次数: 7
The Quantity of Shea Nut Assessed, Collected and Processed Using Improved Shea Nut Processing Technologies in Niger State, Nigeria 尼日利亚尼日尔州利用改进的乳木果加工技术评估、收集和加工乳木果的数量
Pub Date : 2016-01-10 DOI: 10.9734/AJEA/2016/24444
I. Koloche, A. Hamza, A. Mohammed, S. Yahaya, H. Garba, O. Oladipo
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引用次数: 3
Molecular Characterization and Haemolysis of Bacteria Associated with Tilapia zilli from Ijaka-Oke Location on Yewa River 叶瓦河Ijaka-Oke地区齐罗非鱼相关细菌的分子特征及溶血作用
Pub Date : 2016-01-10 DOI: 10.9734/AJEA/2016/19984
O. Oyelakin, A. Aki̇nyemi̇, J. Ekelemu, A. Oloyede, B. Abiona
Fishes are a source of animal protein, rich in minerals, amino acids, and essential fatty acids. Fishes can be confronted with microbial contamination in their natural habitat and their transformation products can be a source of microbial infections and food poisoning. In the natural habitat, microorganisms are usually found on the entire outer surface (skin and gills) and in the intestines of fishes. Genetic diversity study of the bacteria isolates was done using RAPD technique. Bacteria were isolated from the gills, gut and skin of Tilapia zilli from Ijaka-Oke location on Yewa River. Morphometric characteristics of the 20 Tilapia zilli sampled was done. DNA were extracted from 20 bacteria isolates using CTAB method, PCR amplification of the 20 isolated DNA was carried out using five RAPD primers. Haemolysis of the 20 bacteria was also carried out using 7% of sheep blood in Nutrient Agar and it was used to culture a pure colony of the isolates and incubated at 37°C for 18 hours. The mean total length of the fishes was 30.00±9.00 (cm), weight 220±4.3 (g), head length 7.4±2.00 (cm) and standard length 21.30±4.50 (cm). The water quality parameters were also recorded with the water temperature at 22.90C, the pH of the water was 6.1 and Dissolved Oxygen 6.89 mg/l. Fifty – two (52) polymorphic markers were generated from the 5 RAPD markers. There were also 15 monomorphic markers from the primers. A total number of 67 markers were generated. The haemolysis showed that none of the bacteria displayed complete haemolysis.
鱼类是动物蛋白的来源,富含矿物质、氨基酸和必需脂肪酸。鱼类在其自然栖息地可能面临微生物污染,其转化产物可能成为微生物感染和食物中毒的来源。在自然栖息地,微生物通常存在于鱼类的整个外表面(皮肤和鳃)和肠道中。利用RAPD技术对分离菌株进行遗传多样性研究。本文从叶华河Ijaka-Oke地区的罗非鱼鳃、肠道和皮肤中分离出细菌。对20尾罗非鱼进行了形态计量学分析。采用CTAB法从20株分离的细菌中提取DNA,用5条RAPD引物对20株分离的DNA进行PCR扩增。用7%的羊血在营养琼脂中对20种细菌进行溶血,用于培养分离的纯菌落,并在37°C下孵育18小时。平均体长30.00±9.00 (cm),体重220±4.3 (g),头长7.4±2.00 (cm),标准体长21.30±4.50 (cm)。记录了水温为22.90℃,pH为6.1,溶解氧为6.89 mg/l时的水质参数。5个RAPD标记共产生52个多态性标记。引物中也有15个单态标记。共生成67个标记。溶血结果显示,没有一种细菌表现出完全的溶血。
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引用次数: 1
Population Dynamics of the Mango Mealybug Rastrococcus invadens Williams (Homoptera: Pseudococcidea) in Western Burkina Faso 布基纳法索西部芒果粉蚧入侵葡萄球菌种群动态(同翅目:假球虫总目)
Pub Date : 2016-01-10 DOI: 10.9734/AJEA/2016/24819
Karim Nébié, S. Nacro, L. C. Otoidobiga, D. Dakouo, I. Somda
Aims: The study aimed at determining the periods of high pest population of the mango tree mealybug Rastrococcus invadens Williams with respect to biotic and abiotic factors. Study Design: Semiweekly observations were performed on the mango trees with a view of assessing the density of alive, dead or parasitized R. invadens. Original Research Article Nebie et al.; AJEA, 11(6): 1-11, 2016; Article no.AJEA.24819 2 Place and Duration of the Study: This study was conducted in Western Burkina Faso from May 2014 to April 2015. Methodology: A set of mango trees was selected in each of the following locations: Toussiana, Beregadougou and Orodara. The assessment was conducted on 20 leaves (five leaves per cardinal point) that were picked up from each mango tree at each observation date. The parasitized mealybugs were kept inside pill boxes for two weeks and the emerging parasitoids were collected and kept in alcohol 70°. Data were registered and processed with the Microsoft Excel 2010 and SPSS software. Results: Results showed that R. invadens’ populations were more abundant during the wet season (May-September 2014) than the dry season (November 2014-April 2015) in all study locations. In general, parasitism was low. Some peaks of abundance were recorded in both wet and dry seasons. The evolution of the mortality of the insect pest was the same in all locations and peaks were observed from December 2014 to April 2015. The density of living R. invadens was significantly correlated with temperature and relative humidity. Impact of the parasitism due to Anagyrus mangicola Noyes and Gyranusoidea tebygi Noyes were also assessed and discussed. Conclusion: Periods of R. invadens populations’ abundance are well known in Western Burkina Faso. The parasitoids A. mangicola and G. tebygi are well established. The level of parasitism could be improved by releasing of A. mangicola and G. tebygi to control R. invadens efficiently.
目的:从生物和非生物因素的角度确定芒果树粉蚧入侵葡萄球菌的高发期。研究设计:每半周对芒果树进行观察,以评估活的、死的和被寄生的入侵夜蛾的密度。Nebie et al.;农业学报,11(6):1-11,2016;文章no.AJEA。24819研究地点和时间:本研究于2014年5月至2015年4月在布基纳法索西部进行。方法:在下列地点分别选择一组芒果树:图西亚纳、贝雷加杜古和奥罗达拉。评估是在每个观察日期从每棵芒果树上采集的20片叶子(每个基点5片叶子)上进行的。将被寄生的粉蚧置于药盒内保存2周,收集出芽寄生蜂,在70°酒精中保存。数据采用Microsoft Excel 2010和SPSS软件进行登记和处理。结果:各研究点的湿季(2014年5月~ 9月)入侵白颊鼠种群数量均高于干季(2014年11月~ 2015年4月)。总体而言,寄生率较低。一些丰度高峰在旱季和雨季都有记录。各地点害虫死亡率演变规律一致,高峰出现在2014年12月至2015年4月。活桦尺蠖密度与温度和相对湿度呈显著相关。此外,还评价和讨论了芒豆蛾和黄颡鱼蛾的寄生效应。结论:布吉纳法索西部入侵黑腹虫种群的丰度期是已知的。寄生蜂manmangicola A.和tebygi G.已经建立。释放芒草姬螨和特比吉螨可以有效地控制入侵田鼠的寄生水平。
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引用次数: 5
Performance, Nutrient Intake and Digestibility of Uda Sheep with Graded Levels of Xylopia aethiopica (Ethiopian pepper) 不同水平黑胡椒对乌达羊生产性能、营养物质采食量和消化率的影响
Pub Date : 2016-01-10 DOI: 10.9734/AJEA/2016/26581
N. Muhammad, I. Musa, S. Maigandi, S. Buhari, Khalifa Muhammad Aljameel
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引用次数: 0
Effect of Antibiotic and Bio-fungicide for Control of Seed Borne Fungi of Wheat 抗生素与生物杀菌剂对小麦种传真菌的防治效果
Pub Date : 2016-01-10 DOI: 10.9734/AJEA/2016/28988
R. K. Seth, S. Alam, H. Singh, J. Srivastava
The present study aimed to evaluate the effect of antibiotic and bio-fungicide for control of seed borne fungi of wheat during November to April. 2015-2016. In this study, two treatments viz. Control, Aureofungin (Antibiotic) with 100 ml water, and Allium sativum leaf extract with cow urine (Biofungicide) for control of seed borne fungi of wheat, In the pot experiment, The result was obtained as a control 63.20%, and disease incidence of 23.30% which were recorded from Allium sativum leaf extract with cow urine (Bio-fungicide) in the Treatment 3 whereas control 47.41% and disease incidence 33.33% were recorded from 4 gm. Aureofungin (Antibiotic) with 100 water in the Treatment 2. In the pots, the control was 68.75%, and as disease incidence 20% which were recorded from Allium sativum leaf extract with cow urine (Bio-fungicide) in the Treatment 3 whereas control was 52.09% and disease incidence was 30.66% which were recorded from 4 gm. Aureofungin with 100 ml water (Antibiotic) in the T 3. Bio-fungicide was found to be superior in controlling of seed borne fungi of wheat comparing with antibiotic. A bio-fungicide is composed of beneficial microorganisms, such as specialized fungi that attack and control plant pathogens and the diseases. Short Research Article Seth et al.; AJEA, 14(1): 1-5, 2016; Article no.AJEA.28988 2
本研究旨在评价2015-2016年11月至4月期间抗生素和生物杀菌剂对小麦种传真菌的防治效果。本研究采用金毛菌素(抗生素)加100 ml水对照和葱叶提取物加牛尿(生物杀菌剂)两种处理防治小麦种传真菌,盆栽试验结果为对照63.20%。处理3用牛尿(生物杀菌剂)处理的葱叶提取物的发病率为23.30%,而对照处理2用4 gm金黄色真菌素(抗生素)加100水处理的发病率为47.41%,发病率为33.33%。在盆栽试验中,对照为68.75%,病发率为20%,在处理3中,用牛尿(生物杀菌剂)配制葱叶提取物,对照为52.09%,病发率为30.66%,在处理3中,用4 gm金黄色真菌素加100 ml水(抗生素)配制。生物杀菌剂对小麦种传真菌的防治效果优于抗生素。生物杀菌剂是由有益微生物组成的,如攻击和控制植物病原体和疾病的专门真菌。Seth et al.;农业学报,14(1):1-5,2016;文章no.AJEA。28988 2
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引用次数: 0
Vegetable Production Potential of Turkey 土耳其蔬菜生产潜力
Pub Date : 2016-01-10 DOI: 10.9734/AJEA/2016/25778
H. Başak, Melihşah Doğuş
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引用次数: 1
Comparative evaluation of hoe-weeding and Pendimethalin spray regimes on weed management in cowpea (Vigna unguiculata (L) Walp.) in North Central Nigeria. 奈及利亚中北部豇豆(Vigna unguiculata (L) Walp.)杂草管理的锄草和喷甲氧甲烷比较评价。
Pub Date : 2016-01-10 DOI: 10.9734/AJEA/2016/19988
A. Oluwafemi, J. Abiodun
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引用次数: 1
Evaluation of the Spray Droplet Size Spectra of Drift-reducing Agricultural Spray Nozzle Designs 减漂农业喷雾喷嘴设计中喷雾液滴粒径谱的评价
Pub Date : 2016-01-10 DOI: 10.9734/ajea/2016/23785
Joshua McGinty, P. A. Baumann, W. C. Hoffmann, B. Fritz
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引用次数: 8
Impact of Foliar Application of Growth Regulators and Micronutrients on the Performance of Darjeeling Mandarin 叶面施用生长调节剂和微量营养素对大吉岭柑桔生产性能的影响
Pub Date : 2016-01-10 DOI: 10.9734/AJEA/2016/25447
S. Gurung, S. Mahato, C. P. Suresh, Binoy Chetrri
Investigation was carried out in the farmer’s field of Kalimpong to evaluate the effect of foliar application of different level of GA3 and micronutrients on Darjeeling mandarin. The experimental design was adopted randomized block design in which there was seven main plot treatments representing combinations of three growth regulators (GA3 @ 7.5 ppm and 15 ppm, BA @ 200 ppm and 400 ppm and 2,4-D @ 7.5 ppm and 15 ppm) and two micro nutrients (Zn @ 0.5% and Boron @ 0.1%). Foliar application of GA3 at the rate of 15 ppm along with zinc (0.5%) and boron (0.1%) improved growth morphology, fruit yield attributes is also effective in enhancing the fruit yield with better fruit quality. Generally, it could be concluded that the treatment (T3) seems to be the promising treatment for the hilly region of Darjeeling.
为评价叶面施用不同水平GA3和微量营养素对大吉岭柑桔生长的影响,在噶伦堡农民田间进行了调查。试验设计采用随机区组设计,其中有7个主要小区处理,分别代表3种生长调节剂(GA3 @ 7.5 ppm和15 ppm, BA @ 200 ppm和400 ppm, 2,4- d @ 7.5 ppm和15 ppm)和2种微量营养素(Zn @ 0.5%和硼@ 0.1%)的组合。叶面施用15 ppm的GA3,同时施用锌(0.5%)和硼(0.1%),改善了果实的生长形态和产量属性,也有效地提高了果实的产量和品质。总的来说,可以得出结论,T3处理似乎是大吉岭丘陵地区有前途的处理方法。
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引用次数: 15
期刊
American Journal of Experimental Agriculture
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