Brush border membrane vesicles (BBMV) from the midgut epithelial cells of silkworm larvae were prepared. ATP hydrolyzing activity (ATPase activity) was associated with the BBMV. ATPase activity without Mg2 + was not observed at pH 7 but substantial ATP hydrolyzing activity was observed at pH 7 with Mg2 +. The enzyme required Mn2 +, Mg2 +, or Ca2+ ions. The enzyme also hydrolyzed ITP and GTP but not p-NPP, ADP, or AMP. KNO3 and NEM strongly inhibited the ATPase activity. Behaviours of the ATPase against inhibitors suggested that it resembled vacuolar type ATPase.
{"title":"Characterization of ATPase Activity in Brush Border Membrane Vesicles from the Silkworm, Bombyx mori","authors":"M. Minami, L. S. Indrasith, H. Hori","doi":"10.1271/BBB1961.55.2693","DOIUrl":"https://doi.org/10.1271/BBB1961.55.2693","url":null,"abstract":"Brush border membrane vesicles (BBMV) from the midgut epithelial cells of silkworm larvae were prepared. ATP hydrolyzing activity (ATPase activity) was associated with the BBMV. ATPase activity without Mg2 + was not observed at pH 7 but substantial ATP hydrolyzing activity was observed at pH 7 with Mg2 +. The enzyme required Mn2 +, Mg2 +, or Ca2+ ions. The enzyme also hydrolyzed ITP and GTP but not p-NPP, ADP, or AMP. KNO3 and NEM strongly inhibited the ATPase activity. Behaviours of the ATPase against inhibitors suggested that it resembled vacuolar type ATPase.","PeriodicalId":7729,"journal":{"name":"Agricultural and biological chemistry","volume":"24 1","pages":"2693-2700"},"PeriodicalIF":0.0,"publicationDate":"1991-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85974327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Okamoto, S. Kato, T. Kobutani, M. Ogasawara, M. Konnai, T. Takematsu
N-(1-Arylethenyl)-2-chloroacetamides were synthesized and their herbicidal activities were tested. Among them, both 2-chloro-N-(2-methoxyethyl)-N-(2-methyl-1-phenylpropen-1-yl)acetamide and 2-chloro-N(2-ethoxyethyl)-N-(2-methyl-1-phenylpropen-1-yl)acetamide were found to be highly active against upland weeds.
{"title":"Herbicidally Active N-(1-Arylethenyl)-2-chloroacetamides Bearing an Alkyloxyalkyl Moiety","authors":"H. Okamoto, S. Kato, T. Kobutani, M. Ogasawara, M. Konnai, T. Takematsu","doi":"10.1271/BBB1961.55.2737","DOIUrl":"https://doi.org/10.1271/BBB1961.55.2737","url":null,"abstract":"N-(1-Arylethenyl)-2-chloroacetamides were synthesized and their herbicidal activities were tested. Among them, both 2-chloro-N-(2-methoxyethyl)-N-(2-methyl-1-phenylpropen-1-yl)acetamide and 2-chloro-N(2-ethoxyethyl)-N-(2-methyl-1-phenylpropen-1-yl)acetamide were found to be highly active against upland weeds.","PeriodicalId":7729,"journal":{"name":"Agricultural and biological chemistry","volume":"1 1","pages":"2737-2743"},"PeriodicalIF":0.0,"publicationDate":"1991-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73484510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Two novel insecticidal metabolites, calyculin E (1) and F (2), which had insecticial activity against the German cockroach and mosquito larvae, were isolated from a Japanese marine sponge, Discodermia sp. By an extensive examination of the NMR spectra, the isolates were elucidated to be stereoisomers of calyculin A (3) and B (4), respectively. The molecular shape of calyculin E in solution was deduced to be in a folded conformation by using NMR parameters such as NOE values and coupling constants in conjunction with NOE-restricted energy minimization and computer-assisted molecular modeling.
{"title":"Calyculin E and F, Novel Insecticidal Metabolites, from the Marine Sponge, Discodermia sp.","authors":"A. Okada, Keisuke Watanabe, K. Umeda, M. Miyakado","doi":"10.1271/BBB1961.55.2765","DOIUrl":"https://doi.org/10.1271/BBB1961.55.2765","url":null,"abstract":"Two novel insecticidal metabolites, calyculin E (1) and F (2), which had insecticial activity against the German cockroach and mosquito larvae, were isolated from a Japanese marine sponge, Discodermia sp. By an extensive examination of the NMR spectra, the isolates were elucidated to be stereoisomers of calyculin A (3) and B (4), respectively. The molecular shape of calyculin E in solution was deduced to be in a folded conformation by using NMR parameters such as NOE values and coupling constants in conjunction with NOE-restricted energy minimization and computer-assisted molecular modeling.","PeriodicalId":7729,"journal":{"name":"Agricultural and biological chemistry","volume":"88 1","pages":"2765-2771"},"PeriodicalIF":0.0,"publicationDate":"1991-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90360522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Assay for tyrosinase activity using L-dopa as the substrate. The enzyme activity was measured spectrophotometrically by the method of Pomerantz12) with a slight modification. The enzyme solution (0.2ml) was incubated at 25°C with 1.5ml of4mM D,L-dopa in 0.1m sodium phosphate buffer, pH 6.5, containing 0.9% (w/v) NaCl. The conversion of L-dopa to dopachrome was followed by monitoring the increase of absorbance at
{"title":"Studies on phenoloxidase from Mytilus edulis associated with adhesion","authors":"Naohiro Maruyama, H. Etoh, K. Sakata, K. Ina","doi":"10.1271/BBB1961.55.2887","DOIUrl":"https://doi.org/10.1271/BBB1961.55.2887","url":null,"abstract":"Assay for tyrosinase activity using L-dopa as the substrate. The enzyme activity was measured spectrophotometrically by the method of Pomerantz12) with a slight modification. The enzyme solution (0.2ml) was incubated at 25°C with 1.5ml of4mM D,L-dopa in 0.1m sodium phosphate buffer, pH 6.5, containing 0.9% (w/v) NaCl. The conversion of L-dopa to dopachrome was followed by monitoring the increase of absorbance at","PeriodicalId":7729,"journal":{"name":"Agricultural and biological chemistry","volume":"9 5 1","pages":"2887-2889"},"PeriodicalIF":0.0,"publicationDate":"1991-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76824562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
effect of some dietary components on fatty liver by using a model of the fatty liver induced by acute ethanol administration. We often observed no or only a slight lipid accumulation in the liver, even when we administered ethanol by the method reported in the previous paper.1} Wesupposed that one of the reasons might be due to the breeder and/or strain differences in the rats. Male Sprague-Dawley (SD) rats at 5 weeks of age were obtained from Charles River Japan (Kanagawa), Nihon Clea (Tokyo), Nihon SLC (Shizuoka), and Seiwa
{"title":"Effect of strain difference on lipid metabolism in rats after an acute ethanol administration","authors":"S. Mochizuki, A. Yoshida","doi":"10.1271/BBB1961.55.2895","DOIUrl":"https://doi.org/10.1271/BBB1961.55.2895","url":null,"abstract":"effect of some dietary components on fatty liver by using a model of the fatty liver induced by acute ethanol administration. We often observed no or only a slight lipid accumulation in the liver, even when we administered ethanol by the method reported in the previous paper.1} Wesupposed that one of the reasons might be due to the breeder and/or strain differences in the rats. Male Sprague-Dawley (SD) rats at 5 weeks of age were obtained from Charles River Japan (Kanagawa), Nihon Clea (Tokyo), Nihon SLC (Shizuoka), and Seiwa","PeriodicalId":7729,"journal":{"name":"Agricultural and biological chemistry","volume":"61 1","pages":"2895-2896"},"PeriodicalIF":0.0,"publicationDate":"1991-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83971553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Random point mutation analysis of the signal peptide cleavage area of Bacillus stearothermophilus alpha-amylase.","authors":"K Yamaguchi, M Ueda, G Kawanishi, S Udaka","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":7729,"journal":{"name":"Agricultural and biological chemistry","volume":"55 11","pages":"2875-6"},"PeriodicalIF":0.0,"publicationDate":"1991-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12540516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Polysaccharides were extracted from a Chinese mushroom, "Huangmo" with hot water (FI), 1% ammonium oxalate solution (FII), and 5% sodium hydroxide (FIII) and 20 more polysaccharides were isolated by ethanol precipitation, ion-exchange chromatography, gel filtration, and affinity chromatography. Of the six polysaccharides obtained, FI0, FA-1, FA-2, FII-2, FIII-1-b, and FIII-2-b were selected on the basis of the results of the antitumor activity test (the Sarcoma 180/mice, i.p. method). Six fractions which showed relatively high activities were examined: FI0, a mixture of galactose-containing heteroglycan and beta-D-glucan; FA-1 and -2, beta-D-glucan with galactose-containing hetero-glycan and protein; FII-2, beta-D-glucan; and FIII-1-b and FIII-2-b, (1----6)-beta-D-glucosyl branched (1----3)-beta-D-glucan.
采用热水(FI)、1%草酸铵溶液(FII)和5%氢氧化钠溶液(FIII)分别提取黄磨菇多糖，并通过乙醇沉淀、离子交换层析、凝胶过滤和亲和层析等方法分离出20余个多糖。根据抗肿瘤活性试验(Sarcoma 180/小鼠，i.p.法)的结果，从得到的6种多糖中选择FI0、FA-1、FA-2、FII-2、FIII-1-b和FIII-2-b。研究了六个具有较高活性的组分:FI0，含半乳糖的杂聚糖和β - d -葡聚糖的混合物;FA-1和-2，β - d -葡聚糖与含半乳糖的异聚糖和蛋白质;FII-2 beta-D-glucan;以及FIII-1-b和FIII-2-b， (1----6)- β - d -葡萄糖基支链(1----3)- β - d -葡聚糖。
{"title":"Antitumor activity of some polysaccharides isolated from a Chinese mushroom, \"huangmo\", the fruiting body of Hohenbuehelia serotina.","authors":"Y Ma, T Mizuno, H Ito","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Polysaccharides were extracted from a Chinese mushroom, \"Huangmo\" with hot water (FI), 1% ammonium oxalate solution (FII), and 5% sodium hydroxide (FIII) and 20 more polysaccharides were isolated by ethanol precipitation, ion-exchange chromatography, gel filtration, and affinity chromatography. Of the six polysaccharides obtained, FI0, FA-1, FA-2, FII-2, FIII-1-b, and FIII-2-b were selected on the basis of the results of the antitumor activity test (the Sarcoma 180/mice, i.p. method). Six fractions which showed relatively high activities were examined: FI0, a mixture of galactose-containing heteroglycan and beta-D-glucan; FA-1 and -2, beta-D-glucan with galactose-containing hetero-glycan and protein; FII-2, beta-D-glucan; and FIII-1-b and FIII-2-b, (1----6)-beta-D-glucosyl branched (1----3)-beta-D-glucan.</p>","PeriodicalId":7729,"journal":{"name":"Agricultural and biological chemistry","volume":"55 11","pages":"2701-10"},"PeriodicalIF":0.0,"publicationDate":"1991-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12542082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1991-11-01DOI: 10.1080/00021369.1991.10871034
H. Okamoto, S. Kato, M. Ogasawara, M. Konnai, T. Takematsu
{"title":"Synthesis and Herbicidal Activity ofN(1-Arylethenyl)-2-chloroacetamides","authors":"H. Okamoto, S. Kato, M. Ogasawara, M. Konnai, T. Takematsu","doi":"10.1080/00021369.1991.10871034","DOIUrl":"https://doi.org/10.1080/00021369.1991.10871034","url":null,"abstract":"","PeriodicalId":7729,"journal":{"name":"Agricultural and biological chemistry","volume":"109 1","pages":"2733-2736"},"PeriodicalIF":0.0,"publicationDate":"1991-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75476457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1991-11-01DOI: 10.1080/00021369.1991.10871048
K. Murakami, Yutaka Ishida, A. Masaki, Hiroki Tatsumi, Seiji Murakami, E. Nakano, H. Motai, Haruhide Kawabe, Hirofumi Arimura
The genomic DNA for the alkaline protease (Alp) of the fungus Aspergillus oryzae was isolated using synthetic oligonucleotides as hydridization probes, and the complete nucleotide sequence was identified. The Alp gene is 1374 nucleotides long and contains three introns, one of which is in the pro region and two in the mature coding region. Sequences related to the TATA box (TATAAAT) and the CAAT box (CCAAAT) were found in the 5'-noncoding region. Primer extension analysis showed that three transcriptional start points are present.
{"title":"Isolation and characterization of the alkaline protease gene of Aspergillus oryzae.","authors":"K. Murakami, Yutaka Ishida, A. Masaki, Hiroki Tatsumi, Seiji Murakami, E. Nakano, H. Motai, Haruhide Kawabe, Hirofumi Arimura","doi":"10.1080/00021369.1991.10871048","DOIUrl":"https://doi.org/10.1080/00021369.1991.10871048","url":null,"abstract":"The genomic DNA for the alkaline protease (Alp) of the fungus Aspergillus oryzae was isolated using synthetic oligonucleotides as hydridization probes, and the complete nucleotide sequence was identified. The Alp gene is 1374 nucleotides long and contains three introns, one of which is in the pro region and two in the mature coding region. Sequences related to the TATA box (TATAAAT) and the CAAT box (CCAAAT) were found in the 5'-noncoding region. Primer extension analysis showed that three transcriptional start points are present.","PeriodicalId":7729,"journal":{"name":"Agricultural and biological chemistry","volume":"19 1","pages":"2807-11"},"PeriodicalIF":0.0,"publicationDate":"1991-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75061643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y. Konishi, K. Horikawa, Y. Oku, J. Azumaya, N. Nakatani
Two alfiumin fractions were extracted from defatted flours of seeds of Amaranthus hypochondriacus and A. cruentus. One fraction (Alb-1) was extracted with 0.5m NaCl or water when used as the first solvent. Another fraction (Alb-2) was extracted with water after removing Alb-1 and globulin. Apparent pIs of Alb-1 and Alb-2 were 4.5–7.5 and 5–6, respectively. Alb-2 was precipitated by various metal ions (1 mm), while Alb-1 was not. Alb-2 contained four major components (Mr = 56, 36, 26, 22 kD), although Alb-1 had no particular major proteins. Pronase treatment of amaranth flour before protein extraction decomposed Alb-1, but not Alb-2, as seen by SDS-PAGE analysis. The findings that Alb-2 was protected from the Pronase treatment and it could not be extracted until Alb-1 and globulin were removed suggests that Alb-2 might be associated with protein bodies [Irving et al., J. Food Sci., 46, 1170 (1981)].
从苋菜种子脱脂面粉中分别提取了两个alfiumin部分。以0.5m NaCl或水为第一溶剂，提取一份Alb-1。除去Alb-1和球蛋白后，用水提取另一部分(Alb-2)。Alb-1和Alb-2的表观pi分别为4.5 ~ 7.5和5 ~ 6。Alb-2被各种金属离子沉淀(1 mm)，而Alb-1没有。Alb-2含有四种主要成分(Mr = 56、36、26、22 kD)，而Alb-1没有特定的主要蛋白。SDS-PAGE分析表明，蛋白提取前的Pronase处理能分解Alb-1，但不能分解Alb-2。研究发现，Alb-2不受Pronase的影响，在去除Alb-1和球蛋白之前无法提取，这表明Alb-2可能与蛋白体有关[Irving et al.， J. Food Sci.]。[j].农业科学，1997,17(5)。
{"title":"Extraction of Two Albumin Fractions from Amaranth Grains: Comparison of Some Physicochemical Properties and the Putative Localization in the Grains","authors":"Y. Konishi, K. Horikawa, Y. Oku, J. Azumaya, N. Nakatani","doi":"10.1271/BBB1961.55.2745","DOIUrl":"https://doi.org/10.1271/BBB1961.55.2745","url":null,"abstract":"Two alfiumin fractions were extracted from defatted flours of seeds of Amaranthus hypochondriacus and A. cruentus. One fraction (Alb-1) was extracted with 0.5m NaCl or water when used as the first solvent. Another fraction (Alb-2) was extracted with water after removing Alb-1 and globulin. Apparent pIs of Alb-1 and Alb-2 were 4.5–7.5 and 5–6, respectively. Alb-2 was precipitated by various metal ions (1 mm), while Alb-1 was not. Alb-2 contained four major components (Mr = 56, 36, 26, 22 kD), although Alb-1 had no particular major proteins. Pronase treatment of amaranth flour before protein extraction decomposed Alb-1, but not Alb-2, as seen by SDS-PAGE analysis. The findings that Alb-2 was protected from the Pronase treatment and it could not be extracted until Alb-1 and globulin were removed suggests that Alb-2 might be associated with protein bodies [Irving et al., J. Food Sci., 46, 1170 (1981)].","PeriodicalId":7729,"journal":{"name":"Agricultural and biological chemistry","volume":"10 1","pages":"2745-2750"},"PeriodicalIF":0.0,"publicationDate":"1991-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72804507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：