Pub Date : 2024-01-02DOI: 10.1080/22297928.2024.2318298
Thangsim K Sangma, Nokman G Momin, Sengprang R Marak, Gamrik S Sangma, Hari Prasad Jaishi
{"title":"Assessment of heavy metal concentrations in industrial samples near Byrnihat, Meghalaya: Implications for human health","authors":"Thangsim K Sangma, Nokman G Momin, Sengprang R Marak, Gamrik S Sangma, Hari Prasad Jaishi","doi":"10.1080/22297928.2024.2318298","DOIUrl":"https://doi.org/10.1080/22297928.2024.2318298","url":null,"abstract":"","PeriodicalId":7793,"journal":{"name":"Analytical Chemistry Letters","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140514942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-02DOI: 10.1080/22297928.2024.2314527
Vijaykumar K. Parmar, Dipali Mistry, Meghal D. Patel, Dharati Saradhara
{"title":"DoE based development and validation of HPTLC method for simultaneous estimation of Curcumin and Naringin in topical gel formulation","authors":"Vijaykumar K. Parmar, Dipali Mistry, Meghal D. Patel, Dharati Saradhara","doi":"10.1080/22297928.2024.2314527","DOIUrl":"https://doi.org/10.1080/22297928.2024.2314527","url":null,"abstract":"","PeriodicalId":7793,"journal":{"name":"Analytical Chemistry Letters","volume":"167 1-4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140515112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
AbstractIn this work, a simple and innovative fabrication strategy was developed by electrodeposition procedures to combine a film of deposited silver nanoparticles (Ag- NPs) on multi-walled carbon nanotubes to fabricate an Ag- NPs@MWNTC&CPE sensor to detect the active principle of Ronidazole. The physicochemical nature of Ag- NPs@MWNTC&CPE nanocomposite, their texture and morphology were characterized by XRD and SEM. The electrochemical results were performed using cyclic voltammetry, ESI and differential pulse voltammetry techniques. Under optimal experimental conditions, a highly electrocatalytic activity is observed, the reduction reaction of Ronidazole is controlled by a diffusion process with the participation of four electrons and four protons, and a strong potential shift of the reduction peak was measured at -0.58 V/ Ag- NPs@MWNTC&CPE vs Ag/AgCl. The developed sensor shows good selectivity in the presence of metal ions, biomolecules and similar nitro-containing drugs. The results showed that the cathodic current peak was linearly proportional to the concentration of Ronidazole in the range of 1.0×10−4 to 1 ×10−7 M with a detection limit of LOD=2.01×10−8M. To analyze the practicality of the technique for the determination of Ronidazole in real samples of spiked chicken liver and muscle with satisfactory recoveries.Keywords: Multiwalled carbon nanotubeSilver nanoparticlesRonidazoleDifferential pulse voltammetryChicken liverChicken muscle
{"title":"One-step electrodeposition of silver nanoparticles on multi-walled carbon nanotubes to fabricate an Ag-NPs@MWNTC&CPE sensor for the detection of the toxic antibiotic ronidazole in doped chicken liver and muscle","authors":"Jallal Zoubir, Yassine Elkhotfi, Samir Qourzal, Malika Tamimi, Abdessamad Tounsi, Ali Assabbane, Idriss Bakas","doi":"10.1080/22297928.2023.2263761","DOIUrl":"https://doi.org/10.1080/22297928.2023.2263761","url":null,"abstract":"AbstractIn this work, a simple and innovative fabrication strategy was developed by electrodeposition procedures to combine a film of deposited silver nanoparticles (Ag- NPs) on multi-walled carbon nanotubes to fabricate an Ag- NPs@MWNTC&CPE sensor to detect the active principle of Ronidazole. The physicochemical nature of Ag- NPs@MWNTC&CPE nanocomposite, their texture and morphology were characterized by XRD and SEM. The electrochemical results were performed using cyclic voltammetry, ESI and differential pulse voltammetry techniques. Under optimal experimental conditions, a highly electrocatalytic activity is observed, the reduction reaction of Ronidazole is controlled by a diffusion process with the participation of four electrons and four protons, and a strong potential shift of the reduction peak was measured at -0.58 V/ Ag- NPs@MWNTC&CPE vs Ag/AgCl. The developed sensor shows good selectivity in the presence of metal ions, biomolecules and similar nitro-containing drugs. The results showed that the cathodic current peak was linearly proportional to the concentration of Ronidazole in the range of 1.0×10−4 to 1 ×10−7 M with a detection limit of LOD=2.01×10−8M. To analyze the practicality of the technique for the determination of Ronidazole in real samples of spiked chicken liver and muscle with satisfactory recoveries.Keywords: Multiwalled carbon nanotubeSilver nanoparticlesRonidazoleDifferential pulse voltammetryChicken liverChicken muscle","PeriodicalId":7793,"journal":{"name":"Analytical Chemistry Letters","volume":" 3","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135290540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-03DOI: 10.1080/22297928.2023.2277744
A. Orabi, Ahmed H. Ismail, K. Abou El-Nour, Bahig M. Atia, M. Gado, M. Cheira
Abstract Phosphogypsum (PG) is a byproduct of the decomposition of phosphate rock with sulfuric acid during phosphoric acid synthesis. Various techniques such as X-ray diffraction (XRD), Scan electron microscope with electron dispersive spectroscopy (SEM-EDS), Fourier-transform infrared spectroscopy (FTIR), and Thermal gravimetric analysis (TGA) are utilized to characterize PG and rare earth concentrate. Heir, both nitric acid (HNO3) and ammonium nitrate (NH4NO3), are used as leaching agents for rare earth elements (REEs) from Egyptian PG. The ideal working conditions for leaching about 85% of REEs from PG are 350 mg/kg REEs in PG, 149 μm grain size, 0.5M NH4NO3, 2M HNO3, 1/4 S/L, 30 minutes leaching time, 300 rpm, and 80°C. The data on the kinetic REEs leaching are matched to the diffusion control model. The kinetics of the applicable leaching activity and the reaction mechanism between the mixture of nitric acid and ammonium nitrate with REEs have been explored in detail. After concentration by vaporization to one liter, a final concentration of REEs reached ≈ 300 mg/L with a leachability of 85%. The REEs were precipitated using 20% oxalic acid at pH 2.5 as oxalate, and then the precipitate was calcined at 900°C in an electric oven, given a mixture of REEs oxides and CaO. GRAPHICAL ABSTRACT
{"title":"Nitrate-mediated kinetic leaching for rare earth elements recovery from phosphogypsum","authors":"A. Orabi, Ahmed H. Ismail, K. Abou El-Nour, Bahig M. Atia, M. Gado, M. Cheira","doi":"10.1080/22297928.2023.2277744","DOIUrl":"https://doi.org/10.1080/22297928.2023.2277744","url":null,"abstract":"Abstract Phosphogypsum (PG) is a byproduct of the decomposition of phosphate rock with sulfuric acid during phosphoric acid synthesis. Various techniques such as X-ray diffraction (XRD), Scan electron microscope with electron dispersive spectroscopy (SEM-EDS), Fourier-transform infrared spectroscopy (FTIR), and Thermal gravimetric analysis (TGA) are utilized to characterize PG and rare earth concentrate. Heir, both nitric acid (HNO3) and ammonium nitrate (NH4NO3), are used as leaching agents for rare earth elements (REEs) from Egyptian PG. The ideal working conditions for leaching about 85% of REEs from PG are 350 mg/kg REEs in PG, 149 μm grain size, 0.5M NH4NO3, 2M HNO3, 1/4 S/L, 30 minutes leaching time, 300 rpm, and 80°C. The data on the kinetic REEs leaching are matched to the diffusion control model. The kinetics of the applicable leaching activity and the reaction mechanism between the mixture of nitric acid and ammonium nitrate with REEs have been explored in detail. After concentration by vaporization to one liter, a final concentration of REEs reached ≈ 300 mg/L with a leachability of 85%. The REEs were precipitated using 20% oxalic acid at pH 2.5 as oxalate, and then the precipitate was calcined at 900°C in an electric oven, given a mixture of REEs oxides and CaO. GRAPHICAL ABSTRACT","PeriodicalId":7793,"journal":{"name":"Analytical Chemistry Letters","volume":"33 1","pages":"561 - 580"},"PeriodicalIF":0.0,"publicationDate":"2023-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139343033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-03DOI: 10.1080/22297928.2023.2289515
Sridhar Bonam, T. Siva Rao, K. Rama Srinivas, Suman Pallapati
Abstract A fixed combination of Nivolumab and Relatlimab (OPDUALAG) are human monoclonal antibodies (mAb) that are extensively used to treat skin cancer melanoma. The current investigation is to develop a robust, stability-indicating, and rapid method for the simultaneous determination of these two drugs by using the RP-UPLC technique. By utilizing this technique, the final chromatographic conditions were achieved with a Luna C18 column (50 x 2.1 mm, 1.6 μm), and a mobile phase made with acetonitrile and 0.1% formic acid, 6:4 ratio, respectively (Diluted 1.0 mL of Formic acid in 1000 mL of HPLC grade water) by adapting isocratic elution. Both Nivolumab and Relatlimab were detected at 229 nm, and a satisfactory separation was achieved in 3.0 minutes of run time with a flow rate of 0.2 mL/min. The method is linear from 15 - 90 μg/mL for Nivolumab (R2 = 0.9996) and 5 - 30 μg/mL for Relatlimab (R2 = 0.9997). Results of method repeatability for Nivolumab (% RSD=1.3 %) and Relatlimab (% RSD=0.48 %) and intermediate precision Nivolumab (% RSD=1.1 %) and Relatlimab (% RSD=0.48%) were found within acceptable limits. Also, the forced degradation study results show that it is discernible; the degradation products of both drugs were separated from the main peak, which demonstrates the efficiency of the stability-indicating method. So this method works well for routine analysis and stability studies. GRAPHICAL ABSTRACT
摘要 Nivolumab和Relatlimab（OPDUALAG）是人类单克隆抗体（mAb），被广泛用于治疗皮肤癌黑色素瘤。目前的研究旨在利用 RP-UPLC 技术开发一种稳健、稳定、快速的方法，用于同时测定这两种药物。采用该技术的最终色谱条件为：Luna C18色谱柱（50 x 2.1 mm, 1.6 μm），流动相为乙腈和0.1%甲酸，比例为6:4（在1000 mL HPLC级水中稀释1.0 mL甲酸），采用等度洗脱。Nivolumab和Relatlimab的检测波长均为229 nm，流速为0.2 mL/min，运行时间为3.0分钟，分离效果令人满意。Nivolumab 的线性范围为 15 - 90 μg/mL（R2 = 0.9996），Relatlimab 的线性范围为 5 - 30 μg/mL（R2 = 0.9997）。Nivolumab（RSD%=1.3%）和Relatlimab（RSD%=0.48%）的方法重复性结果以及Nivolumab（RSD%=1.1%）和Relatlimab（RSD%=0.48%）的中间精密度结果均在可接受范围内。此外，强制降解研究结果表明，两种药物的降解产物均可从主峰中分离出来，这表明该稳定性指示方法的效率很高。因此，该方法可用于常规分析和稳定性研究。图表摘要
{"title":"Determination of human monoclonal antibodies nivolumab and relatlimab in opdualag by using the RP-UPLC technique: method development and validation","authors":"Sridhar Bonam, T. Siva Rao, K. Rama Srinivas, Suman Pallapati","doi":"10.1080/22297928.2023.2289515","DOIUrl":"https://doi.org/10.1080/22297928.2023.2289515","url":null,"abstract":"Abstract A fixed combination of Nivolumab and Relatlimab (OPDUALAG) are human monoclonal antibodies (mAb) that are extensively used to treat skin cancer melanoma. The current investigation is to develop a robust, stability-indicating, and rapid method for the simultaneous determination of these two drugs by using the RP-UPLC technique. By utilizing this technique, the final chromatographic conditions were achieved with a Luna C18 column (50 x 2.1 mm, 1.6 μm), and a mobile phase made with acetonitrile and 0.1% formic acid, 6:4 ratio, respectively (Diluted 1.0 mL of Formic acid in 1000 mL of HPLC grade water) by adapting isocratic elution. Both Nivolumab and Relatlimab were detected at 229 nm, and a satisfactory separation was achieved in 3.0 minutes of run time with a flow rate of 0.2 mL/min. The method is linear from 15 - 90 μg/mL for Nivolumab (R2 = 0.9996) and 5 - 30 μg/mL for Relatlimab (R2 = 0.9997). Results of method repeatability for Nivolumab (% RSD=1.3 %) and Relatlimab (% RSD=0.48 %) and intermediate precision Nivolumab (% RSD=1.1 %) and Relatlimab (% RSD=0.48%) were found within acceptable limits. Also, the forced degradation study results show that it is discernible; the degradation products of both drugs were separated from the main peak, which demonstrates the efficiency of the stability-indicating method. So this method works well for routine analysis and stability studies. GRAPHICAL ABSTRACT","PeriodicalId":7793,"journal":{"name":"Analytical Chemistry Letters","volume":"26 1","pages":"528 - 538"},"PeriodicalIF":0.0,"publicationDate":"2023-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139343072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-03DOI: 10.1080/22297928.2023.2271024
Pranita S. Kanojiya, Rita Wadetwar, Amit Godbole, Nilesh P. Gawande, Samiksha S. Dadmal, Adesh L. Fasate, Chandu B. Karad
Abstract Vaginal Candidiasis had caused quite frequent gynecological medical consultations. Fluconazole is an antifungal drug that has poor solubility that hinders dissolution and antifungal activity. The study aimed to formulate the sustained release vaginal tablet of Fluconazole solid dispersion through the Design of Experiment approach to guarantee no compromise with the quality. In the first step, Plackett Burman Design was employed for the preliminary screening of the essential factors that would affect the tablet’s property. The significant factors were used further in applying the 32 factorial design and the responses were studied. The drug release, bioadhesive strength and swelling index were taken as the responses and considered as the vital critical quality attributes of the vaginal tablet. The design space was determined through the contour and response surface plots. The tablet was prepared within the design space with the drug release (99.97±0.1%), bioadhesive strength (1671.62±43.23 dynes/cm2), and swelling index (342.47±5 %) that suggested the values lie within the quality target product profile determined previously. The antifungal activity against C. albicans resulted in a larger inhibition zone for the solid dispersion-based tablet (51±3 mm) than the plain drug-based tablet (36±2 mm). GRAPHICAL ABSTRACT
{"title":"Quality by design approach in the formulation of vaginal tablet of fluconazole solid dispersion","authors":"Pranita S. Kanojiya, Rita Wadetwar, Amit Godbole, Nilesh P. Gawande, Samiksha S. Dadmal, Adesh L. Fasate, Chandu B. Karad","doi":"10.1080/22297928.2023.2271024","DOIUrl":"https://doi.org/10.1080/22297928.2023.2271024","url":null,"abstract":"Abstract Vaginal Candidiasis had caused quite frequent gynecological medical consultations. Fluconazole is an antifungal drug that has poor solubility that hinders dissolution and antifungal activity. The study aimed to formulate the sustained release vaginal tablet of Fluconazole solid dispersion through the Design of Experiment approach to guarantee no compromise with the quality. In the first step, Plackett Burman Design was employed for the preliminary screening of the essential factors that would affect the tablet’s property. The significant factors were used further in applying the 32 factorial design and the responses were studied. The drug release, bioadhesive strength and swelling index were taken as the responses and considered as the vital critical quality attributes of the vaginal tablet. The design space was determined through the contour and response surface plots. The tablet was prepared within the design space with the drug release (99.97±0.1%), bioadhesive strength (1671.62±43.23 dynes/cm2), and swelling index (342.47±5 %) that suggested the values lie within the quality target product profile determined previously. The antifungal activity against C. albicans resulted in a larger inhibition zone for the solid dispersion-based tablet (51±3 mm) than the plain drug-based tablet (36±2 mm). GRAPHICAL ABSTRACT","PeriodicalId":7793,"journal":{"name":"Analytical Chemistry Letters","volume":"69 1","pages":"505 - 527"},"PeriodicalIF":0.0,"publicationDate":"2023-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139343075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-03DOI: 10.1080/22297928.2023.2283451
Shariqah Hijazi, Nayeem Ahmed, H. Louis, Imojara Anna, W. A. Shah
Abstract In this study, we carried out the isolation of gallic acid from Hippophae rhamnoides plant followed by synthetic modification by using Dicyclohexyl carbodimide (DCC) as a coupling reagent. The synthetic modification involves the amidation of Gallic acid by a one-step protocol, converting gallic acid to its corresponding amide derivatives. The reactions involve a simple one-pot procedure with the formation of a chain of scaffolds of various beneficial amides in good to outstanding yields. The synthesized amides were characterized by several spectroscopic techniques like FTIR, HNMR, and Mass spectrometry. The synthetic derivatives were theoretically studied by Density Functional Theory (DFT) and their biological structural feasibility was studied by docking studies. Moreover, the antibacterial activities of the derivatives were also evaluated, and it is worthwhile to mention that all compounds have shown good antibacterial activity with the highest inhibition zone of 21 mm (A2) against strains of gram-positive and gram-negative bacteria with MIC value of 40 mg/mL. GRAPHICAL ABSTRACT
摘要 在这项研究中，我们从鼠李糖植物中分离出没食子酸，然后用二环己基碳二亚胺（DCC）作为偶联试剂进行合成修饰。合成改性包括一步法酰胺化没食子酸，将没食子酸转化为相应的酰胺衍生物。反应采用简单的一步法进行，可形成一连串各种有益酰胺的支架，产率从良好到卓越。傅立叶变换红外光谱、氢核磁共振和质谱等光谱技术对合成的酰胺进行了表征。通过密度泛函理论（DFT）对合成的衍生物进行了理论研究，并通过对接研究对其生物结构的可行性进行了研究。此外，还对这些衍生物的抗菌活性进行了评估，值得一提的是，所有化合物都表现出了良好的抗菌活性，对革兰氏阳性菌和革兰氏阴性菌菌株的最高抑菌区为 21 mm (A2)，MIC 值为 40 mg/mL。图表摘要
{"title":"Biological and computational studies of novel scaffolds of gallic acid: insight from density functional theory and molecular docking studies","authors":"Shariqah Hijazi, Nayeem Ahmed, H. Louis, Imojara Anna, W. A. Shah","doi":"10.1080/22297928.2023.2283451","DOIUrl":"https://doi.org/10.1080/22297928.2023.2283451","url":null,"abstract":"Abstract In this study, we carried out the isolation of gallic acid from Hippophae rhamnoides plant followed by synthetic modification by using Dicyclohexyl carbodimide (DCC) as a coupling reagent. The synthetic modification involves the amidation of Gallic acid by a one-step protocol, converting gallic acid to its corresponding amide derivatives. The reactions involve a simple one-pot procedure with the formation of a chain of scaffolds of various beneficial amides in good to outstanding yields. The synthesized amides were characterized by several spectroscopic techniques like FTIR, HNMR, and Mass spectrometry. The synthetic derivatives were theoretically studied by Density Functional Theory (DFT) and their biological structural feasibility was studied by docking studies. Moreover, the antibacterial activities of the derivatives were also evaluated, and it is worthwhile to mention that all compounds have shown good antibacterial activity with the highest inhibition zone of 21 mm (A2) against strains of gram-positive and gram-negative bacteria with MIC value of 40 mg/mL. GRAPHICAL ABSTRACT","PeriodicalId":7793,"journal":{"name":"Analytical Chemistry Letters","volume":"48 1","pages":"487 - 504"},"PeriodicalIF":0.0,"publicationDate":"2023-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139343102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-04DOI: 10.1080/22297928.2023.2263018
R. Surya, Manohar D. Mullassery, Noeline B. Fernandez, D. Thomas
Abstract In this work, a novel alginate-modified Na-Bentonite clay composite (APTSB-AL) was synthesized via an ionotropic gelation technique for the controlled delivery of 5-Flurouracil. Characterization of the composite was performed using Fourier transform infrared spectroscopy (FTIR): X-ray diffraction (XRD): scanning electron microscopy (SEM) etc. In order to acquire the targeted drug delivery, the composite was covered by chitosan-folate conjugate (APTSB-AL-CSFA). The freeze-drying method was adopted to avoid the leakage of water-soluble drug molecules during the drying process. The maximum swelling of the composite was found to be at pH 6.8 and the maximum drug release was at pH 7.4. The release kinetics mechanism was explained by the Korsmeyer-Peppas kinetic model. In order to find out the material toxicity, the biocompatibility assay in a normal cell line of mouse fibroblast (L929) was carried out. At the concentration of 2.5 μg/ml, the cell toxicity was negligible. At the high concentration level of 40.0 μg/ml, the cell viability was not less than 70.0 %. The in vitro cytotoxicity of the drug-loaded composite was studied in Human Colorectal Adenocarcinoma cells and the findings indicated good biocompatibility of the composite. At the concentration of 2.5 μg/ml of the drug-loaded composite (FU-L-APTSB-AL-CSFA): the cell toxicity was found to be less than 50%. And at the high concentration level of 40.0 μg/ml, the cell viability was found to be less than 10%, indicating the targeting action of the loaded composite. GRAPHICAL ABSTRACT
{"title":"Alginate-bentonite composite decorated by chitosan-folate conjugate for the oral delivery of 5-Fluorouracil","authors":"R. Surya, Manohar D. Mullassery, Noeline B. Fernandez, D. Thomas","doi":"10.1080/22297928.2023.2263018","DOIUrl":"https://doi.org/10.1080/22297928.2023.2263018","url":null,"abstract":"Abstract In this work, a novel alginate-modified Na-Bentonite clay composite (APTSB-AL) was synthesized via an ionotropic gelation technique for the controlled delivery of 5-Flurouracil. Characterization of the composite was performed using Fourier transform infrared spectroscopy (FTIR): X-ray diffraction (XRD): scanning electron microscopy (SEM) etc. In order to acquire the targeted drug delivery, the composite was covered by chitosan-folate conjugate (APTSB-AL-CSFA). The freeze-drying method was adopted to avoid the leakage of water-soluble drug molecules during the drying process. The maximum swelling of the composite was found to be at pH 6.8 and the maximum drug release was at pH 7.4. The release kinetics mechanism was explained by the Korsmeyer-Peppas kinetic model. In order to find out the material toxicity, the biocompatibility assay in a normal cell line of mouse fibroblast (L929) was carried out. At the concentration of 2.5 μg/ml, the cell toxicity was negligible. At the high concentration level of 40.0 μg/ml, the cell viability was not less than 70.0 %. The in vitro cytotoxicity of the drug-loaded composite was studied in Human Colorectal Adenocarcinoma cells and the findings indicated good biocompatibility of the composite. At the concentration of 2.5 μg/ml of the drug-loaded composite (FU-L-APTSB-AL-CSFA): the cell toxicity was found to be less than 50%. And at the high concentration level of 40.0 μg/ml, the cell viability was found to be less than 10%, indicating the targeting action of the loaded composite. GRAPHICAL ABSTRACT","PeriodicalId":7793,"journal":{"name":"Analytical Chemistry Letters","volume":"56 1","pages":"461 - 474"},"PeriodicalIF":0.0,"publicationDate":"2023-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139362924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-04DOI: 10.1080/22297928.2023.2252425
Heru Agung Saputra, Arif Ashari, Md Mobarok Karim, Md Ali Zaber Sahin, Khatun A. Jannath
Abstract Lung cancer is among the most life-threatening diseases, as it is by far the leading cause of death around the globe. However, plentiful studies have been reported with satisfactory results employing electrochemical biosensors, which make this method popular in lung cancer diagnosis. In assembling the sensing platforms, different materials of the sensor interfaces are utilized in electrode surface modification to enhance performance. A natural biopolymer chitosan (CS) is an outstanding material for the development of electrochemical biosensors. The unique physiochemical features exhibited by CS facilitate the preservation of its innovative traits and promote its secure adhesion onto the electrode surface. It also has inherent characteristics, such as high adsorption ability, good biocompatibility, eco-friendliness nature, greater heat stability, improved permeability, mechanical potency, outstanding layer-forming capability, and ease of availability. In combination with conductive materials, CS enables the modified electrodes to perform the best analytical results. This review is focused on the importance of CS in sensor configuration, advancements of CS-based electrochemical biosensors for detecting lung cancer biomarkers, and future prospects. GRAPHICAL ABSTRACT
{"title":"Chitosan-based electrochemical biosensors for lung cancer detection: A mini-review","authors":"Heru Agung Saputra, Arif Ashari, Md Mobarok Karim, Md Ali Zaber Sahin, Khatun A. Jannath","doi":"10.1080/22297928.2023.2252425","DOIUrl":"https://doi.org/10.1080/22297928.2023.2252425","url":null,"abstract":"Abstract Lung cancer is among the most life-threatening diseases, as it is by far the leading cause of death around the globe. However, plentiful studies have been reported with satisfactory results employing electrochemical biosensors, which make this method popular in lung cancer diagnosis. In assembling the sensing platforms, different materials of the sensor interfaces are utilized in electrode surface modification to enhance performance. A natural biopolymer chitosan (CS) is an outstanding material for the development of electrochemical biosensors. The unique physiochemical features exhibited by CS facilitate the preservation of its innovative traits and promote its secure adhesion onto the electrode surface. It also has inherent characteristics, such as high adsorption ability, good biocompatibility, eco-friendliness nature, greater heat stability, improved permeability, mechanical potency, outstanding layer-forming capability, and ease of availability. In combination with conductive materials, CS enables the modified electrodes to perform the best analytical results. This review is focused on the importance of CS in sensor configuration, advancements of CS-based electrochemical biosensors for detecting lung cancer biomarkers, and future prospects. GRAPHICAL ABSTRACT","PeriodicalId":7793,"journal":{"name":"Analytical Chemistry Letters","volume":"42 1","pages":"337 - 354"},"PeriodicalIF":0.0,"publicationDate":"2023-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139363323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-04DOI: 10.1080/22297928.2023.2263015
A. Neelaveni, K. Senthil, K. Anbazhakan, S.V. Tharan prabu, T. Preethi, N. Sivakumar
Abstract In this article, a neem gum-based polymer electrolyte is synthesised using the solution casting approach. XRD, FTIR, and AC impedance analysis are used to investigate the structural and electrical properties of bio-gel polymer electrolytes (BGPEs) that have been synthesized. According to XRD analysis, the film is more amorphous at 60 wt % PVA: 20 wt % NG: 20 wt % NaCF3SO3 (BP2). The observed amorphous nature is due to a greater number of Na+ and CF3SF3– ions coordinated with the polymer side chains’ COO– and OH– groups. The FTIR analysis revealed the disappearance of CH2 wagging of pure Poly (vinyl alcohol) (PVA) and shifting of stretching vibration mode of (C – N) aromatic functional group of protein of pure neem gum (NG) due to complexation of blended polymer (PVA: NG) with Na ions. The conductivity of the produced films is analysed using the conductance spectra, and the film BP2 (60 wt % PVA: 20 wt % NG: 20 wt % NaCF3SO3) is found to have the highest ionic conductivity (≈ 5.328 x 10-4 Scm-1) at 303 K. Flexibility, hopping mechanism are well enhanced in host polymer (PVA: NG) due to the addition of ionic salt NaCF3SO3, which is clearly observed in the dielectric spectra. GRAPHICAL ABSTRACT
{"title":"An investigation on conductivity and dielectric behaviour of neem gum blended PVA biopolymer electrolytes","authors":"A. Neelaveni, K. Senthil, K. Anbazhakan, S.V. Tharan prabu, T. Preethi, N. Sivakumar","doi":"10.1080/22297928.2023.2263015","DOIUrl":"https://doi.org/10.1080/22297928.2023.2263015","url":null,"abstract":"Abstract In this article, a neem gum-based polymer electrolyte is synthesised using the solution casting approach. XRD, FTIR, and AC impedance analysis are used to investigate the structural and electrical properties of bio-gel polymer electrolytes (BGPEs) that have been synthesized. According to XRD analysis, the film is more amorphous at 60 wt % PVA: 20 wt % NG: 20 wt % NaCF3SO3 (BP2). The observed amorphous nature is due to a greater number of Na+ and CF3SF3– ions coordinated with the polymer side chains’ COO– and OH– groups. The FTIR analysis revealed the disappearance of CH2 wagging of pure Poly (vinyl alcohol) (PVA) and shifting of stretching vibration mode of (C – N) aromatic functional group of protein of pure neem gum (NG) due to complexation of blended polymer (PVA: NG) with Na ions. The conductivity of the produced films is analysed using the conductance spectra, and the film BP2 (60 wt % PVA: 20 wt % NG: 20 wt % NaCF3SO3) is found to have the highest ionic conductivity (≈ 5.328 x 10-4 Scm-1) at 303 K. Flexibility, hopping mechanism are well enhanced in host polymer (PVA: NG) due to the addition of ionic salt NaCF3SO3, which is clearly observed in the dielectric spectra. GRAPHICAL ABSTRACT","PeriodicalId":7793,"journal":{"name":"Analytical Chemistry Letters","volume":"9 1","pages":"403 - 415"},"PeriodicalIF":0.0,"publicationDate":"2023-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139363411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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