Analytical Sciences最新文献_第5页

Solid-phase extraction of ethyleneamines in river water prior to their determination by high-performance liquid chromatography with ultraviolet detection 采用高效液相色谱-紫外检测法对河水中乙胺进行固相萃取测定。

IF 2 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences

Pub Date : 2025-10-02 DOI: 10.1007/s44211-025-00850-5

Azusa Yudo, Tomoko Kemmei, Shuji Kodama, Yoshinori Inoue, Shigehiro Kagaya

A method based on solid-phase extraction (SPE) has been developed for the preconcentration of three kinds of ethyleneamines (EAs) [ethylenediamine (EDA), diethylenetriamine (DETA), and triethylenetetramine (TETA)] in river water for their high-performance liquid chromatographic (HPLC) determination by using on-line complexation with Cu(II) ion and ultraviolet (UV) detection. EAs, which are protonated in acidic and neutral conditions, could be concentrated by using cation-exchange SPE. However, inorganic cations and humic substances, often present in river water, interfered with this preconcentration. Inorganic cations, such as Ca and Mg ions, compete with EAs in cation-exchange processes. This interference could be reduced by masking them with ethylenediaminetetraacetic acid. Humic substances, such as humic acid and fluvic acid, seem to be due to the electrostatic interaction of EAs with them in the sample solution. This interference was reduced by passing the sample solution through an anion-exchange cartridge before the cation-exchange SPE. The interacted EAs remained in the anion-exchange cartridge; they could be eluted by washing out the anion-exchange cartridge with diluted HCl. In this washing process, although fulvic acid was eluted at the same time, it could be removed by passing the eluate through a reversed-phase cartridge connected in a series. After removing these interferences, EAs could be extraced with the cation-exchange SPE and quantitatively eluted by passing Cu(II) solution through the cartridge. The proposed method of including the HPLC–UV combined with the SPE was available for the determination of EAs in river water at concentrations as low as 0.015 µM for EDA and DETA and 0.05 µM for TETA. The high recoveries (83%–103%) and repeatabilities (RSD 1.2%–4.3%) were obtained for EAs from two river water samples spiked with two different concentrations of EAs. The proposed method was applied to analyses of river water samples taken from four rivers in Toyama Prefecture, Japan.

Graphical Abstract

建立了基于固相萃取（SPE）的三种乙二胺[乙二胺（EDA）、二乙三胺（DETA）和三乙四胺（TETA）]在河水中预富集的高效液相色谱(HPLC) - Cu（II）离子在线络合和紫外（UV）检测方法。EAs在酸性和中性条件下均被质子化，可通过阳离子交换SPE进行富集。然而，无机阳离子和腐殖质，通常存在于河水，干扰这种预浓缩。无机阳离子，如Ca和Mg离子，在阳离子交换过程中与ea竞争。用乙二胺四乙酸掩盖可以减少这种干扰。腐植酸和流酸等腐植酸物质似乎是由于ea与样品溶液中的静电相互作用而产生的。在进行阳离子交换固相萃取之前，将样品溶液通过阴离子交换筒来减少这种干扰。相互作用的ea留在阴离子交换盒中；它们可以通过用稀释的盐酸冲洗阴离子交换筒来洗脱。在此洗涤过程中，虽然黄腐酸是同时洗脱的，但可以通过将洗脱液通过串联的反相滤筒除去。去除这些干扰后，可以用阳离子交换固相萃取萃取ea，并用Cu（II）溶液通过滤筒定量洗脱。建立的高效液相色谱-紫外联用固相萃取法可用于测定河水中EDA和DETA浓度低至0.015µM， TETA浓度低至0.05µM的EAs。两种不同浓度的ea在两种河流水样中均具有较高的回收率（83% ~ 103%）和重复性（RSD 1.2% ~ 4.3%）。所提出的方法应用于分析取自日本富山县四条河流的水样。

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引用次数: 0

Virus inactivation effect of hypochlorite solution containing nitrate ions 含硝酸盐离子的次氯酸盐溶液的病毒灭活效果。

IF 2 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences

Pub Date : 2025-10-01 DOI: 10.1007/s44211-025-00831-8

Erina Satake, Maho Ishiyama, Masaki Sekiguchi, Atsushi Sasaki, Shigekazu Yano, Masatoshi Endo

Hypochlorous acid (HClO) is widely used as a disinfectant due to its strong oxidizing power, but its efficacy is highly dependent on pH and decreases under neutral to alkaline conditions. In addition, its high concentration poses safety and environmental concerns. In this study, we investigated the enhancement of virucidal activity at lower HClO concentrations through the formation of monochloramine (NH₂Cl) by supplementing with ammonium-form nitrogen (NH₄⁺–N) or nitrate-form nitrogen (NO₃⁻–N) under different pH conditions. Using bacteriophage φ6 as an enveloped virus model, plaque assays revealed that virus inactivation was most effective at pH 8 when 15–20 ppm of NH₄⁺–N was added to 30 ppm HClO solution, with over 90% reduction in infectivity. Under acidic conditions (pH 6), the formation of NH₂Cl was limited due to the rapid consumption of free chlorine and insufficient generation of NH₃, resulting in reduced virucidal efficacy; however, increasing the amount of NH₄⁺–N led to improved inactivation effects. In contrast, at pH 8, NH₂Cl formation was enhanced, contributing to improved disinfection efficiency. Similar effects were observed when NO₃⁻–N was used, likely due to its reduction to NH₄⁺ in the presence of organic matter. These results demonstrate that optimizing pH and nitrogen sources can improve the efficacy of low-concentration HClO solutions by generating NH₂Cl in situ, offering a safer and more sustainable approach to viral disinfection.

Graphical abstract

次氯酸（HClO）因其强大的氧化能力而被广泛用作消毒剂，但其效果高度依赖于pH值，在中性至碱性条件下会下降。此外，它的高浓度引起了安全和环境问题。在本研究中，我们研究了在不同的pH条件下，通过补充铵态氮（NH4+-N）或硝酸盐态氮（NO3——N）形成一氯胺（NH2Cl），在低HClO浓度下增强病毒的杀病毒活性。以φ6噬菌体为包膜病毒模型，在pH为8时，将15-20 ppm的NH4+-N加入到30 ppm的HClO溶液中，病毒灭活效果最好，传染性降低90%以上。在酸性条件下（pH 6），由于游离氯的快速消耗和NH₃的生成不足，NH2Cl的形成受到限制，导致杀病毒效果降低；然而，增加NH4+-N的用量可以改善失活效果。相反，在pH为8时，NH2Cl的形成增强，有助于提高消毒效率。当使用NO3—N时，观察到类似的效果，可能是由于它在有机物存在下还原成NH₄⁺。这些结果表明，优化pH和氮源可以通过原位生成NH2Cl来提高低浓度HClO溶液的消毒效果，为病毒消毒提供了一种更安全、更可持续的方法。

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引用次数: 0

Advances in microfluidic chip technology for cell analysis 用于细胞分析的微流控芯片技术进展。

IF 2 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences

Pub Date : 2025-09-30 DOI: 10.1007/s44211-025-00851-4

Luxi Shu, Yan Zhang

Microfluidic chips play a crucial role in the field of cell analysis. These miniature chips integrate seamlessly across key cell analysis stages, including sample preparation, cell culture, sorting, lysis, and detection. They possess many advantages such as miniaturization, integration, automation, and portability. The size of their microchannels is comparable to that of cells, thus enabling research to be conducted at the single-cell or even subcellular organelle level. They can also simulate the physiological conditions in vivo, conduct non-destructive or minimally destructive detections, and meet the requirements of high-throughput cell analysis, which is beneficial for parallel operations and continuous analysis. This article reviews the technological progress of microfluidic chips, focusing on three major directions: cell sorting and enrichment, single-cell analysis, and dynamic microenvironment simulation. The article also analyzes the challenges faced by this technology, such as cell damage control, handling of multicellular heterogeneity, data interpretation, etc., and proposes coping strategies such as the development of new biomaterials, multimodal integration technology, and artificial intelligence assistance. By combining cutting-edge technologies such as nanotechnology, 3D printing, and organoid culture, the functions of microfluidic chips can be further expanded to enable the simulation and analysis of more complex biological systems and provide important technical support for cell biology research and clinical translation.

Graphical Abstract

微流控芯片在细胞分析领域起着至关重要的作用。这些微型芯片无缝集成在关键的细胞分析阶段，包括样品制备，细胞培养，分选，裂解和检测。它们具有许多优点，如小型化、集成化、自动化和可移植性。它们的微通道大小与细胞相当，因此可以在单细胞甚至亚细胞细胞器水平上进行研究。还可以模拟体内生理条件，进行无损或最小破坏性检测，满足高通量细胞分析的要求，有利于并行操作和连续分析。本文综述了微流控芯片的技术进展，重点介绍了细胞分选富集、单细胞分析和动态微环境模拟三个主要方向。文章还分析了该技术面临的挑战，如细胞损伤控制、多细胞异质性处理、数据解释等，并提出了开发新型生物材料、多模态集成技术、人工智能辅助等应对策略。通过结合纳米技术、3D打印、类器官培养等前沿技术，可以进一步扩展微流控芯片的功能，使其能够模拟和分析更复杂的生物系统，为细胞生物学研究和临床翻译提供重要的技术支持。

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引用次数: 0

Recent topics in X-ray absorption spectroscopy: integrating theory, instrumentation, and applications x射线吸收光谱学的最新课题：集成理论、仪器和应用

IF 2 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences

Pub Date : 2025-09-26 DOI: 10.1007/s44211-025-00840-7

Daitaro Ishikawa

引用次数: 0

Rolling circle transcription-based fluorescent aptasensor for highly sensitive and onsite detection of microcystin-LR 基于滚动环转录的荧光适体传感器，用于高灵敏度和现场检测微囊藻毒素。

IF 2 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences

Pub Date : 2025-09-19 DOI: 10.1007/s44211-025-00839-0

Shuran Yang, Yuling Xu, Cheng Zhang, Ke Xu, Zhiyong Hu, Zhiqiang Xia

Onsite and sensitive detection of microcystin-LR (MC-LR) is crucial for monitoring environmental pollution and protecting human health, particularly in the context of increasing cyanobacterial harmful algal blooms (CyanoHABs). Herein, this study reports a rolling circle transcription (RCT)-based fluorescent aptasensor designed for rapid and quantitative onsite detection of MC-LR in real water samples. Aptamers specific to MC-LR were immobilized onto magnetic beads (MBs) and hybridized with blocker complementary DNA strands to form the MB aptasensor complex. Upon recognition and binding of MC-LR molecules in water samples, the blocker DNA strands were displaced and subsequently cyclized into circular DNA structures via T4 DNA ligase-mediated ligation. These circular DNA templates initiated RCT, generating abundant fluorescent Mango RNA reporters. The resulting fluorescent signals exhibited a robust positive correlation with MC-LR concentration, with a detection limit of 39 pM and a linear range from 0.01 to 3000 nM, demonstrating high sensitivity and specificity. The aptasensor showed excellent specificity against structural analogs and common interferents. Recovery tests in Haihe River samples yielded rates between 98.33% and 101.17%, with relative standard deviations below 5%. This sensing platform offers a simple operational procedure, minimal sample preparation, and excellent applicability for real environmental water monitoring. Thus, the developed RCT-based aptasensor provides a promising, cost-effective alternative for onsite MC-LR detection and contributes significantly to environmental safety and public health management.

Graphical abstract

微囊藻毒素lr （MC-LR）的现场灵敏检测对于监测环境污染和保护人类健康至关重要，特别是在蓝藻有害藻华（CyanoHABs）增加的背景下。本研究报道了一种基于滚动环转录（RCT）的荧光适体传感器，用于快速定量现场检测真实水样中的MC-LR。将MC-LR特异性适配体固定在磁珠（MB）上，并与阻滞剂互补DNA链杂交，形成MB适配体传感器复合物。在水样中识别和结合MC-LR分子后，阻断剂DNA链被移位，随后通过T4 DNA连接酶介导的连接环化成环状DNA结构。这些环状DNA模板启动了RCT，产生了大量的荧光芒果RNA报告基因。结果表明，荧光信号与MC-LR浓度呈正相关，检测限为39 pM，线性范围为0.01 ~ 3000 nM，具有较高的灵敏度和特异性。该配体传感器对结构类似物和常见干扰物具有良好的特异性。海河样品回收率为98.33% ~ 101.17%，相对标准偏差小于5%。该传感平台提供了一个简单的操作程序，最少的样品制备，和优秀的适用性，真正的环境水监测。因此，所开发的基于rct的感应传感器为现场MC-LR检测提供了一种有前途的、具有成本效益的替代方案，并为环境安全和公共卫生管理做出了重大贡献。

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引用次数: 0

A new approach for determining trace platinum concentrations in macroalgae and evaluating tissue-specific distribution 测定大型藻类中痕量铂浓度和评估组织特异性分布的新方法。

IF 2 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences

Pub Date : 2025-09-18 DOI: 10.1007/s44211-025-00849-y

Ziwei Li, Asami S. Mashio, Kuo H. Wong, Hiroshi Hasegawa

Platinum (Pt), extensively used in automotive catalytic converters and anti-cancer drugs, has led to elevated concentrations in the environment. Monitoring Pt levels in coastal ecosystems is crucial as excessive accumulation may cause ecotoxicological impacts. In this study, Undaria pinnatifida, a common edible macroalgae in Japan known for its high affinity for platinum group elements, was evaluated as a biomonitor for monitoring Pt concentrations and assessing its anthropogenic influences in coastal areas. An analytical protocol using inductively coupled plasma mass spectrometry (ICP-MS) was developed and optimized for Pt quantification in macroalgal tissues. The optimal digestion conditions included 0.24 mL/g of hydrofluoric acid and the addition of 7 mL/g of hydrochloric acid, which effectively reduced white precipitate formation and improved Pt recovery. The established method demonstrated excellent sensitivity, achieving a method blank of 16 ppt and a detection limit of 5 ppt, surpassing previously reported approaches. Applying this optimized protocol, tissue-specific Pt concentrations were determined in different parts of U. pinnatifida collected from various coastal zones. The results revealed significant spatial variations in Pt concentrations among plant organs: concentrations in blades, stipes, and sporophylls decreased from the outer bay to the inner bay, while holdfasts concentrations exhibited the opposite trend. These findings suggest distinct Pt accumulation behaviors between above-ground and below-ground tissues of U. pinnatifida, underscoring its potential for monitoring spatial variations of Pt in coastal environments.

Graphical abstract

铂（Pt）广泛用于汽车催化转化器和抗癌药物，导致环境中的浓度升高。监测沿海生态系统中的铂水平至关重要，因为过量积累可能会造成生态毒理学影响。在本研究中，以对铂族元素具有高亲和力的日本常见食用大型藻类裙带藻（Undaria pinnatifida）为研究对象，将其作为监测沿海地区Pt浓度和评估其人为影响的生物监测仪。建立并优化了电感耦合等离子体质谱（ICP-MS）用于大藻组织中铂的定量分析方案。最佳消化条件为氢氟酸浓度为0.24 mL/g，盐酸浓度为7 mL/g，可有效减少白色沉淀的形成，提高铂的回收率。所建立的方法具有优异的灵敏度，方法空白为16ppt，检出限为5ppt，超过了以往报道的方法。应用该优化方案，测定了不同沿海地区采集的裙带菜不同部位的组织特异性铂浓度。结果表明，各器官间Pt浓度存在显著的空间差异：叶片、茎杆和孢子叶的Pt浓度由外湾向内湾递减，而叶柄的Pt浓度则呈现相反的趋势。这些研究结果表明，裙带菜在地上和地下组织中有不同的Pt积累行为，强调了其在沿海环境中监测Pt空间变化的潜力。

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引用次数: 0

Statistical quantification of SERS signals in microfluidic flow using AuNP-bound polystyrene microparticles 利用aunp结合聚苯乙烯微粒统计量化微流态中SERS信号。

IF 2 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences

Pub Date : 2025-09-09 DOI: 10.1007/s44211-025-00846-1

Shu Mingcong, Akihisa Miyagawa, Shoji Ishizaka

Surface-enhanced Raman scattering (SERS) is a powerful analytical technique; however, its quantitative application has been limited by the instability of substrates and significant signal fluctuations. In this study, we demonstrated that 4-aminobenzenethiol (4-ATP) can be quantitatively detected through statistical analysis of SERS signal intensity distributions obtained using citrate-stabilized AuNPs, biotin-functionalized AuNPs, and gold nanoparticle (AuNP)-bound polystyrene (PS) microparticles. Raman spectra obtained in bulk aqueous solution under static conditions showed that the detection sensitivity of 4-ATP using AuNP-bound PS microparticles was approximately twice that achieved with citrate-stabilized AuNPs or biotin-modified AuNPs. Furthermore, the detection sensitivity of 4-ATP was enhanced by introducing AuNP-bound PS microparticles into a microfluidic chip and delivering an aqueous 4-ATP solution under controlled flow conditions. Analysis of the flow rate dependence of SERS signal intensity revealed that the optimal detection sensitivity for 4-ATP was achieved at a flow rate of 0.66 μL·min⁻¹, with a corresponding detection limit of 1.9 μM under these conditions. These results demonstrate that AuNP-bound PS microparticles introduced into the microfluidic chip serve as efficient SERS substrates, enabling highly sensitive and quantitative detection under flow conditions.

Graphical abstract

表面增强拉曼散射（SERS）是一种强大的分析技术；然而，由于衬底的不稳定性和显著的信号波动，其定量应用受到限制。在本研究中，我们通过统计分析柠檬酸盐稳定AuNPs、生物素功能化AuNPs和金纳米颗粒（AuNP）结合聚苯乙烯（PS）微粒获得的SERS信号强度分布，证明了4-氨基苯乙醇（4-ATP）可以被定量检测。静态条件下在体水溶液中获得的拉曼光谱表明，使用aunp结合的PS微粒检测4-ATP的灵敏度大约是柠檬酸稳定的AuNPs或生物素修饰的AuNPs的两倍。此外，通过将aunp结合的PS微粒引入微流控芯片，并在控制流动条件下输送4-ATP水溶液，提高了4-ATP的检测灵敏度。流量分析依赖的ser信号强度显示的最优检测灵敏度4-ATP实现流量的0.66 μL·敏⁻1,对应的检测极限为1.9μM在这些条件下。这些结果表明，引入微流控芯片的aunp结合的PS微粒可以作为高效的SERS底物，在流动条件下实现高灵敏度和定量检测。

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引用次数: 0

Optimization and application of pretreatment for the analysis of typical per- and polyfluoroalkyl substances (PFAAs) in drinking water: a systematic evaluation of filter membranes and SPE Sorbents 饮用水中典型的全氟烷基和多氟烷基物质（PFAAs）分析的预处理优化与应用：过滤膜和SPE吸附剂的系统评价。

IF 2 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences

Pub Date : 2025-09-09 DOI: 10.1007/s44211-025-00847-0

Duwang Li, Wanyuan He, Lingling Liu, Wanlu Ren, Yiqi Wan, Huiping Deng, Hongwei Yang

The accurate detection of trace perfluoroalkyl acids (PFAAs) in drinking water remains challenging due to nonspecific adsorption losses during pretreatment. This study systematically evaluated the adsorption behaviors of 11 PFAAs across five filtration membranes and four solid-phase extraction (SPE) sorbents to establish an optimized analytical protocol. Results demonstrated that glass fiber (GL) filters minimized PFAAs retention (94.85% recovery in aqueous matrices) through electrostatic repulsion, outperforming other membranes such as polypropylene(PP). Among SPE sorbents, Weak Anion Exchange (WAX) exhibited the highest recovery rates among the sorbents evaluated (100.04% ± 2.74% recovery) via a dual adsorption mechanism combining amine-mediated ion exchange and hydrophobic interactions, achieving 30% higher efficiency than Hydrophile-Lipophile Balance (HLB) sorbents. The optimized method achieved low detection limits (LOD: 0.016–0.324 ng/L), high accuracy (spiked recovery: 80.12–115.76%) and precision (RSD < 10%). Method application revealed significantly lower PFAAs contamination in reservoir-sourced finished water compared to river/lake-derived supplies in the mid-Yangtze River region. This work effectively mitigates critical pretreatment biases in PFAAs analysis and provides a reliable solution to address the urgent need for monitoring PFAAs contamination in drinking water systems.

Graphic abstract

由于预处理过程中的非特异性吸附损失，饮用水中痕量全氟烷基酸（PFAAs）的准确检测仍然具有挑战性。本研究系统地评估了11种PFAAs在5种过滤膜和4种固相萃取（SPE）吸附剂上的吸附行为，以建立优化的分析方案。结果表明，玻璃纤维（GL）过滤器通过静电斥力将PFAAs的保留率降至最低（在水基中回收率为94.85%），优于聚丙烯（PP）等其他膜。在SPE吸附剂中，弱阴离子交换（WAX）通过胺离子交换和疏水相互作用的双重吸附机制，回收率最高（100.04%±2.74%），比亲水-亲脂平衡（HLB）吸附剂的回收率高30%。优化后的方法检出限低（LOD: 0.016 ~ 0.324 ng/L），准确度高（加标回收率：80.12 ~ 115.76%），精密度高（RSD）

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引用次数: 0

Determination of phenylphosphonic acid zinc salt in poly(lactic acid) using thermally assisted methylation pyrolysis–gas chromatography/mass spectrometry 热辅助甲基化热解-气相色谱/质谱法测定聚乳酸中苯基膦酸锌盐。

IF 2 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences

Pub Date : 2025-09-08 DOI: 10.1007/s44211-025-00845-2

Kazuko Matsui, Makoto Niwa, Atsushi Watanabe, Chuichi Watanabe, Norio Teramae

Biomass-based polymers such as poly(lactic acid) (PLA) have attracted much attention, because they are renewable, biocompatible, and nontoxic to the environment and have been used in various fields such as biomedical, agricultural, and food packaging industries. However, one of the common drawbacks of PLA-based materials is their low glass transition temperature in the amorphous state, while adding phenylphosphonic acid zinc salt (PPA-Zn) as a nucleating agent was found to be a promising method to improve the physical property of PLA. On the other hand, degradation of PLA-based materials in the environment may cause the pollution from the metal of a nucleating agent in PLA and quantification of nucleating agents in polymers is of interest. Polar phosphonic acid, a ligand in PPA-Zn, is not favorable for the analysis by gas chromatography/mass spectrometry (GC/MS). Such compounds containing polar groups are known to be effectively analyzed by GC/MS in combination with thermally assisted methylation reaction. In this study, we report on determination of PPA-Zn in PLA by coupling thermally assisted methylation with pyrolysis–GC/MS.

Graphical abstract

聚乳酸（PLA）等生物质基聚合物因其可再生、生物相容性好、对环境无毒等特点而受到广泛关注，在生物医学、农业、食品包装等领域得到了广泛应用。然而，PLA基材料的一个共同缺点是其非晶态玻璃化转变温度低，而添加苯基膦酸锌盐（PPA-Zn）作为成核剂被发现是一种很有前途的方法来改善PLA的物理性能。另一方面，聚乳酸基材料在环境中的降解可能会引起聚乳酸中成核剂的金属污染，聚合物中成核剂的量化是一个有趣的问题。极性膦酸是PPA-Zn中的配体，不利于气相色谱/质谱分析。这些含有极性基团的化合物已知可以通过GC/MS结合热辅助甲基化反应进行有效分析。在本研究中，我们报道了热辅助甲基化-热解-气相色谱/质谱联用法测定PLA中PPA-Zn。

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引用次数: 0

Advances in methods for rapid detection of Staphylococcus aureus and methicillin-resistant S. aureus 金黄色葡萄球菌和耐甲氧西林金黄色葡萄球菌快速检测方法研究进展。

IF 2 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences

Pub Date : 2025-09-06 DOI: 10.1007/s44211-025-00843-4

Linlin Zhuang, Mengling Zhu, Xinya Chen, Xiaoming Liu, Tianyu Yuan, Chuang Zhou, Xue Lian, Yunming Xu, Yu Zhang, Qiuping Shen

Staphylococcus aureus (S. aureus) and methicillin-resistant S. aureus (MRSA) are important pathogens that are closely associated with hospital-acquired and community-acquired infections. Rapid and accurate detection methods are important for early diagnosis and treatment of diseases. This paper reviews the latest research progress in the field of rapid detection of S. aureus and MRSA, including chromogenic media, immunological detection methods (e.g., enzyme-linked immunosorbent assays, lateral flow assays, colorimetric/fluorescent immunoassays, etc.), nucleic acid tests (including polymerase chain reaction-based methods, isothermal amplification techniques), biosensors, and novel emerging methods, etc. The advantages and disadvantages of these methods are systematically analyzed and discussed. Future research should focus on improving analytical sensitivity and specificity, shortening detection time, reducing detection cost, and promoting the integration of multiple platforms and intelligent analysis, especially for the development of technologies for point-of-care testing. These efforts are crucial to addressing the pressing need for rapid clinical diagnosis.

Graphical abstract

金黄色葡萄球菌（S. aureus）和耐甲氧西林金黄色葡萄球菌（MRSA）是与医院获得性和社区获得性感染密切相关的重要病原体。快速准确的检测方法对于疾病的早期诊断和治疗至关重要。本文综述了金黄色葡萄球菌和MRSA快速检测领域的最新研究进展，包括显色介质、免疫检测方法（如酶联免疫吸附法、侧流法、比色/荧光免疫法等）、核酸检测方法（包括基于聚合酶链反应的方法、等温扩增技术）、生物传感器和新兴方法等。对这些方法的优缺点进行了系统的分析和讨论。未来的研究应着眼于提高分析的灵敏度和特异性，缩短检测时间，降低检测成本，促进多平台的集成和智能分析，特别是开发护理点检测技术。这些努力对于解决快速临床诊断的迫切需要至关重要。

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引用次数: 0