Analytical Sciences最新文献

英文中文

Numerical simulation of microfluidic droplet shooter for generation of femtoliter-picoliter droplets in gas phase 微流控液滴喷射器在气相中产生飞升-皮升液滴的数值模拟。

IF 2 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences

Pub Date : 2025-12-23 DOI: 10.1007/s44211-025-00859-w

Xin Jiang, Masafumi Watanabe, Yutaka Kazoe

A comprehensive understanding of fluid behavior in microchannels is crucial for the precise generation of liquid droplets, which has become increasingly important with the development of microfluidic technologies. Our group has previously developed a microfluidic droplet shooter that generates fL–pL liquid droplets utilizing two-step gas/liquid flow focusing. However, due to the complicated channel geometry of the droplet shooter, the fabrication process is complicated, and the characterization of these systems remains inadequate. The present study focuses on the computational modeling of liquid droplet generation using a microfluidic droplet shooter that utilizes gas-liquid two-phase flow. A computational model is established to delineate the complex flow within the shooter. Additionally, we reveal the characteristics of liquid droplet generation in the gas phase. We further investigate the design optimization of the microfluidic droplet shooter for generating fL–pL liquid droplets. We propose and analyze several designs, such as optimizing the angles of the gas channels and the length of the liquid column. Faithful to the dimensions and physics of a particular microfluidic droplet shooter, the simulations provide precise predictions in droplet formation. By systematically analyzing the effects of geometric configurations, this research provides valuable design guidance to improve the performance of devices used in areas such as biotechnology, analytical chemistry, and life sciences.

Graphical abstract

全面了解微通道中的流体行为对于精确生成液滴至关重要，随着微流控技术的发展，这一点变得越来越重要。我们的团队之前开发了一种微流体液滴射器，它利用两步气/液流动聚焦产生fL-pL液滴。然而，由于液滴喷射器的通道几何形状复杂，制造工艺复杂，对这些系统的表征仍然不足。本文研究了利用气液两相流的微流控液滴喷射器产生液滴的计算模型。建立了一个计算模型来描述射击器内部的复杂流动。此外，我们还揭示了液滴在气相中产生的特征。我们进一步研究了微流控液滴喷射器的设计优化，以产生fL-pL液滴。我们提出并分析了几种设计方案，如优化气通道的角度和液柱的长度。该模拟忠实于特定微流体液滴射器的尺寸和物理特性，提供了液滴形成的精确预测。通过系统地分析几何结构的影响，本研究为提高生物技术、分析化学和生命科学等领域的设备性能提供了有价值的设计指导。图形抽象

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引用次数: 0

Zirconium-based metal–organic frameworks for pathogen detection 用于病原体检测的锆基金属有机框架。

IF 2 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences

Pub Date : 2025-12-22 DOI: 10.1007/s44211-025-00858-x

Xiaoqing Cao, Yurou Kang, JingJing Li, Menqiu Xiong, Zhenlin Nie, Bangshun He

Pathogenic microorganisms pose severe threats to global public health, demanding rapid and accurate detection strategies. This review comprehensively summarizes recent advances in zirconium-based metal–organic frameworks (Zr-MOFs) for pathogen separation and biosensing. Leveraging high stability, large surface area, and ease of binding to biomolecules, Zr-MOFs-functionalized magnetic composites enable efficient enrichment via specific aptamer binding or label-free adsorption. In biosensing, they serve as versatile platforms: enhancing electrochemical sensitivity with conductive materials/enzymes; offering excellent fluorescence performance via quenching properties, tunable ligands or doped metals; and enabling rapid visual detection through colorimetric characteristics and peroxidase-like activity. Ratiometric signals further enhance reliability in complex matrices, and dual-signal systems expand their application scope. In addition, multimode signal output platforms based on Zr-MOFs are evolving to meet the needs of different situations. These technologies are fast, sensitive, economical, and continue to evolve toward point-of-care testing, offering promising solutions to the challenges of pathogen diagnosis.

Graphical abstract

病原微生物对全球公共卫生构成严重威胁，需要快速和准确的检测战略。本文综述了近年来锆基金属有机框架（Zr-MOFs）在病原菌分离和生物传感方面的研究进展。利用高稳定性、大表面积和易于与生物分子结合的特点，zr - mofs功能化的磁性复合材料可以通过特异性适配体结合或无标记吸附实现高效富集。在生物传感中，它们作为多功能平台：增强导电材料/酶的电化学灵敏度；通过猝灭特性、可调配体或掺杂金属提供优异的荧光性能；并通过比色特性和过氧化物酶样活性实现快速视觉检测。比率信号进一步提高了在复杂矩阵中的可靠性，双信号系统扩大了其应用范围。此外，基于zr - mof的多模信号输出平台也在不断发展，以满足不同情况的需求。这些技术快速、敏感、经济，并不断向即时检测方向发展，为病原体诊断的挑战提供了有希望的解决方案。

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引用次数: 0

Determination of hydrogen peroxide in commercial glycerin using electron spin resonance and chemiluminescence assay 用电子自旋共振和化学发光法测定商品甘油中的过氧化氢。

IF 2 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences

Pub Date : 2025-12-22 DOI: 10.1007/s44211-025-00861-2

Gotaro Shiota, Noriko Tajima, Hidetoshi Arakawa, Masaru Kato

Glycerin is widely used in pharmaceuticals, cosmetics, and foods. In recent years, it has been mainly derived from plant-based materials, particularly palm oil. The most abundant polyphenol in palm oil is 3-O-caffeoylshikimic acid (CSA). Polyphenols reportedly react with oxygen to generate hydrogen peroxide (H₂O₂). In this study, we investigated the generation of H₂O₂ from CSA and palm-derived glycerin using the bis(2,4,6-trichlorophenyl)oxalate (TCPO) chemiluminescence method and electron spin resonance spectroscopy. H₂O₂ concentrations in the range of 10⁻⁴ to 10⁻⁶ mol/L were detected in commercial glycerin samples. In addition, the H₂O₂ concentrations in a commercial glycerin varied widely between batches, even among samples of glycerin produced by the same company. However, the concentrations were approximately 1/1000 of that permitted in disinfectants used in pharmaceuticals, indicating extremely low biological toxicity. Furthermore, the TCPO chemiluminescence method proved to be highly sensitive and precise, even in viscous glycerin samples, showing potential for application in quality control of glycerin formulations and related industrial products.

甘油广泛用于药品、化妆品和食品中。近年来，它主要来自植物性材料，特别是棕榈油。棕榈油中最丰富的多酚是3- o -咖啡酰莽草酸（CSA）。据报道，多酚与氧反应生成过氧化氢（H2O2）。在本研究中，我们利用二（2,4,6-三氯苯基）草酸酯（TCPO）化学发光法和电子自旋共振光谱研究了CSA和棕榈衍生甘油生成H2O2的过程。在商品甘油样品中检测到的H2O2浓度范围为10-4 ~ 10-6 mol/L。此外，商品甘油中H2O2浓度在不同批次之间差异很大，甚至在同一公司生产的甘油样品中也是如此。然而，其浓度约为制药用消毒剂允许浓度的千分之一，表明生物毒性极低。此外，TCPO化学发光方法即使在粘性甘油样品中也具有很高的灵敏度和精度，在甘油配方和相关工业产品的质量控制中具有应用潜力。

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引用次数: 0

Thermochemolysis-gas chromatographic detection of oxidized fatty acids in a single Drosophila melanogaster individual 热化学分解-气相色谱法检测单个黑腹果蝇个体的氧化脂肪酸。

IF 2 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences

Pub Date : 2025-12-16 DOI: 10.1007/s44211-025-00860-3

Yasuyuki Ishida, Misaki Haba, Noa Komoda, Nobuo Kido, Atsushi Watanabe

Thermochemolysis-gas chromatography–mass spectrometry (Thermochemolysis-GC–MS) was applied to detect fatty acid oxidation products in a single Drosophila melanogaster individual (dry weight: 100–400 µg). Linoleic acid oxidized under UV irradiation was first analyzed to identify characteristic oxidation products, including azelaic acid. Using tetramethylammonium hydroxide (TMAH) as an organic alkali reagent, azelaic acid was successfully detected as its dimethyl ester in D. melanogaster individuals reared on linoleic acid–supplemented diets. The use of m/z 185 as a diagnostic ion in extracted ion chromatograms allowed for sensitive determination of azelaic acid in control and linoleic acid–treated D. melanogaster individuals. The treated groups exhibited notable increases in azelaic acid content, which demonstrates the method’s ability to evaluate statistically significant differences based on individual-level data.

Graphical abstract

采用热化学裂解-气相色谱-质谱联用（热化学裂解-气相色谱-质谱联用）技术检测单个黑腹果蝇个体（干重100-400µg）的脂肪酸氧化产物。首先分析了亚油酸在紫外线照射下氧化的特征产物，包括壬二酸。用四甲基氢氧化铵（TMAH）作为有机碱试剂，在添加亚油酸饲料饲养的黑腹天牛个体中成功检测出壬二酸的二甲酯。利用m/ z185作为提取离子色谱的诊断离子，可以灵敏地测定对照和亚油酸处理的黑腹天牛个体中壬二酸的含量。处理组的壬二酸含量显著增加，这表明该方法有能力评估基于个体水平数据的统计学显著差异。

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引用次数: 0

Enhancing peroxidase-like activity of Cu/Ag nanoclusters nanozyme by G/C-rich sequence for colorimetric determination of ochratoxin A 富G/ c序列增强Cu/Ag纳米团簇纳米酶过氧化物酶样活性测定赭曲霉毒素A

IF 2 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences

Pub Date : 2025-12-16 DOI: 10.1007/s44211-025-00857-y

Shaojie Xia, Zhenyuan Yang, Xiaohui Sun, Junjie Huang, Kuan Peng, Yonghong Wang

Ochratoxin A (OTA), a highly toxic mycotoxin, extensively contaminates a wide range of food commodities. Given increasing demands for sensitive and user-friendly detection platforms, we developed a colorimetric OTA biosensor leveraging copper-silver nanoclusters (Cu/Ag NCs), whose peroxidase-like activity is augmented via G/C-rich DNA hybridization. The system employs two tailored DNA strands: a template strand featuring a cytosine-rich domain for NC synthesis flanked by terminal recognition segments and an enhancer strand containing a guanine-rich signal amplification domain and a complementary linker. Upon OTA binding with the aptamer, the apt-template complex dissociates, facilitating template-enhancer hybridization to form G/C-rich double-stranded DNA. This structural transition substantially enhances the peroxidase-like activity of DNA-Cu/Ag NCs, accelerating the oxidation of 3,3′,5,5′-tetramethylbenzidine by H₂O₂ while simultaneously amplifying the colorimetric signal. Quantitative OTA detection is accomplished by monitoring this dynamic color response. The biosensor demonstrates a semi-log linear detection range from 5.0 nM to 1.0 μM with a detection limit of 5.0 nM. Furthermore, the specific test has high selectivity for other common mycotoxins, and its applicability has been validated through real sample analysis, suggesting that the DNA-Cu/Ag NCs biosensor used for OTA detection holds promising practical applications in food samples.

Graphical abstract

赭曲霉毒素A （OTA）是一种剧毒真菌毒素，广泛污染各种食品。鉴于对敏感和用户友好型检测平台的需求日益增长，我们开发了一种利用铜银纳米簇（Cu/Ag纳米簇）的比色OTA生物传感器，其过氧化物酶样活性通过富含G/ c的DNA杂交增强。该系统采用两条定制的DNA链：一条模板链具有用于NC合成的富含胞嘧啶的结构域，两侧为终端识别片段，另一条增强子链包含富含鸟嘌呤的信号放大结构域和互补连接子。当OTA与适体结合时，apt-模板复合物解离，促进模板增强子杂交形成富含G/ c的双链DNA。这种结构转变大大增强了DNA-Cu/Ag NCs的过氧化物酶样活性，加速了H2O2对3,3',5,5'-四甲基联苯胺的氧化，同时放大了比色信号。定量OTA检测是通过监测这种动态颜色响应来完成的。该传感器的半对数线性检测范围为5.0 nM ~ 1.0 μM，检测限为5.0 nM。此外，该特异性检测对其他常见真菌毒素具有较高的选择性，并通过实际样品分析验证了其适用性，表明用于OTA检测的DNA-Cu/Ag NCs生物传感器在食品样品中具有良好的实际应用前景。

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引用次数: 0

Aggregation-induced emission luminogens for volatile organic compound detection 用于挥发性有机化合物检测的聚集诱导发射发光光源。

IF 2 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences

Pub Date : 2025-12-10 DOI: 10.1007/s44211-025-00856-z

Muneeba Amin, Iqra Gulzar, Qurashi Najmu Saqib, Muzaffer Ahmad, Adil Majeed Rather, Nusrat Shafi, Waseem A. Wani, Jahangir Ahmad Rather, Waheed Ahmad Khanday, Abdul Haleem Wani, Akhtar Hussain Malik, Faiza Habib

Volatile organic compounds (VOCs) are crucial components in many daily-use products, yet they pose a significant threat to public health and the environment, even in minute amounts, due to their high toxicity. Therefore, it is crucial to develop a reliable method that could track these hazardous vapours with high sensitivity and selectivity. Among various techniques, fluorescence-based detection strategies have demonstrated great efficiency in both qualitative and quantitative monitoring of VOCs in real-time. These methods are attractive due to their low cost and portability, making them suitable for on-site applications. This review explores the latest advancements in aggregation-induced emission (AIE)-based fluorescent probes tailored for VOC detection across diverse platforms, highlighting their selectivity, sensitivity, and real-world applications. The use of AIE events has been justified for the development of selective and sensitive sensors for VOC detection. Furthermore, the present work investigates the various sensing mechanisms involved in predicting the AIE nature of the fluorophores. Additionally, we discuss existing challenges and propose future directions for designing next-generation AIE-based VOC sensors with enhanced specificity, stability, and practical usability. Coupled with the latest advancements, AIE-based fluorescence sensing of VOCs could contribute to the structural design of future smart VOC sensors that are highly efficient and effective.

Graphical abstract

挥发性有机化合物（VOCs）是许多日用产品中的重要成分，但由于其高毒性，即使是极少量，也会对公众健康和环境构成重大威胁。因此，开发一种可靠的方法，以高灵敏度和高选择性地跟踪这些有害蒸汽是至关重要的。在各种技术中，基于荧光的检测策略在实时定性和定量监测挥发性有机化合物方面表现出很高的效率。这些方法因其低成本和可移植性而具有吸引力，使其适合现场应用。本文综述了基于聚合诱导发射（AIE）的荧光探针的最新进展，这些荧光探针专门用于各种平台上的VOC检测，重点介绍了它们的选择性、灵敏度和实际应用。使用AIE事件已被证明是合理的选择性和敏感的传感器用于VOC检测的发展。此外，本研究还研究了预测荧光团AIE性质所涉及的各种传感机制。此外，我们讨论了现有的挑战，并提出了设计下一代基于ai的VOC传感器的未来方向，这些传感器具有更高的特异性、稳定性和实用性。结合最新进展，基于人工智能的VOCs荧光传感可以为未来高效、高效的智能VOC传感器的结构设计做出贡献。

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引用次数: 0

Recent development of surface treatment of polytetrafluoroethylene 聚四氟乙烯表面处理的最新进展

IF 2 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences

Pub Date : 2025-11-25 DOI: 10.1007/s44211-025-00853-2

Ryoichi Ishimatsu

引用次数: 0

Development of surface-functionalized power-free microchip for breast cancer cell-derived extracellular vesicle detection 用于乳腺癌细胞源性细胞外囊泡检测的表面功能化无功率微芯片的研制。

IF 2 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences

Pub Date : 2025-11-20 DOI: 10.1007/s44211-025-00854-1

Ryo Ishihara, Ryo Matsui, Tadaaki Nakajima, Hinako Yokohari, Ren Ogata, Kotomi Katori, Kazuo Hosokawa, Mizuo Maeda, Akihiko Kikuchi

Extracellular vesicles (EVs) have emerged as promising biomarkers for liquid biopsies because of their responsiveness to various physiological conditions. However, their dynamic physical properties pose significant analytical challenges, which necessitates the development of novel detection methods. In this study, a portable surface-functionalized power-free microchip (SF-PF microchip) was developed that enables the detection of EVs without external pumps. In particular, the SF-PF microchip specifically detected EVs derived from metastatic breast cancer cells with a limit of detection of 5.0 × 10¹⁰ particles/mL. Moreover, the SF-PF microchip required only 2.0 µL of sample volume and completed the detection within approximately 20 min, offering a rapid and convenient approach for EV analysis. Furthermore, the SF-PF microchip successfully detected EVs in serum samples, which highlights its potential for clinical application in liquid biopsies.

Graphical abstract

细胞外囊泡（EVs）因其对各种生理条件的反应性而成为液体活检中有前途的生物标志物。然而，它们的动态物理性质给分析带来了重大挑战，这就需要开发新的检测方法。在这项研究中，开发了一种便携式表面功能化无电源微芯片（SF-PF微芯片），可以在没有外部泵的情况下检测电动汽车。特别是，SF-PF微芯片特异性检测转移性乳腺癌细胞衍生的ev，检测限为5.0 × 1010颗粒/mL。此外，SF-PF微芯片只需要2.0µL的样品体积，并在大约20分钟内完成检测，为EV分析提供了一种快速方便的方法。此外，SF-PF微芯片成功检测血清样本中的ev，这突出了其在液体活检中的临床应用潜力。

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引用次数: 0

Sample pretreatment approaches for improving versatility and convenience in gas chromatographic analysis 提高气相色谱分析通用性和方便性的样品前处理方法

IF 2 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences

Pub Date : 2025-11-10 DOI: 10.1007/s44211-025-00848-z

Yasuyuki Ishida

引用次数: 0

Biomineralized gold nanoparticle-based colorimetric sensing platform for ascorbic acid detection 基于生物矿化金纳米粒子的抗坏血酸检测比色传感平台。

IF 2 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences

Pub Date : 2025-10-08 DOI: 10.1007/s44211-025-00852-3

Zhuqing Wang, Junyuan Zhang, Yuetong Ma, Mengran Zhang, Huixue Zhang, Bing Yang, Qiaorong Tang

Ascorbic acid (AA), a critical regulator of redox homeostasis, plays a vital role in disease prevention. Thus, the development of a rapid and precise detection method is urgently needed. In this work, a colorimetric sensing platform based on bioinspired mineralization-synthesized gold nanoparticles (AuNPs) was proposed for the visual and rapid detection of AA. The as-prepared AuNPs exhibited uniform morphology and excellent oxidase-like activity, efficiently catalyzing 3,3',5,5'-tetramethylbenzidine (TMB) oxidation to produce a characteristic 650 nm absorption peak. However, in the presence of AA, TMB oxidation was significantly inhibited due to the reducing property of AA. The color change was visually detectable within 5 min. The absorbance at 650 nm linearly decreased with the AA concentration in the range of 1–60 μM (R² = 0.999). The detection limit was estimated to 0.27 μM. Besides, the selectivity was excellent against common interfering species. The detection of AA in complex fruit samples was realized, and the recovery rate was 98.5–106.1%. Owing to the simplicity, cost-effectiveness, high sensitivity, and rapid response, this colorimetric sensing strategy provides new insights into the on-site monitoring of antioxidants in biological samples.

Graphical abstract

抗坏血酸（AA）是氧化还原稳态的关键调节因子，在疾病预防中起着重要作用。因此，迫切需要开发一种快速、精确的检测方法。本文提出了一种基于生物激发矿化合成金纳米粒子（AuNPs）的比色传感平台，用于视觉和快速检测AA。所制备的AuNPs具有均匀的形态和优异的类氧化酶活性，能有效催化3,3',5,5'-四甲基联苯胺（TMB）氧化，产生650 nm的特征吸收峰。然而，在AA存在下，由于AA的还原性，TMB的氧化被显著抑制。肉眼可在5分钟内检测到颜色变化。在650 nm处吸光度随AA浓度在1 ~ 60 μM范围内线性降低（R2 = 0.999）。检出限为0.27 μM。此外，对常见的干扰物质具有良好的选择性。实现了复杂水果样品中AA的检测，回收率为98.5 ~ 106.1%。由于简单、经济、高灵敏度和快速响应，这种比色传感策略为生物样品中抗氧化剂的现场监测提供了新的见解。

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