Analytical Sciences最新文献_第4页

Optimization and application of pretreatment for the analysis of typical per- and polyfluoroalkyl substances (PFAAs) in drinking water: a systematic evaluation of filter membranes and SPE Sorbents 饮用水中典型的全氟烷基和多氟烷基物质（PFAAs）分析的预处理优化与应用：过滤膜和SPE吸附剂的系统评价。

IF 2 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences

Pub Date : 2025-09-09 DOI: 10.1007/s44211-025-00847-0

Duwang Li, Wanyuan He, Lingling Liu, Wanlu Ren, Yiqi Wan, Huiping Deng, Hongwei Yang

The accurate detection of trace perfluoroalkyl acids (PFAAs) in drinking water remains challenging due to nonspecific adsorption losses during pretreatment. This study systematically evaluated the adsorption behaviors of 11 PFAAs across five filtration membranes and four solid-phase extraction (SPE) sorbents to establish an optimized analytical protocol. Results demonstrated that glass fiber (GL) filters minimized PFAAs retention (94.85% recovery in aqueous matrices) through electrostatic repulsion, outperforming other membranes such as polypropylene(PP). Among SPE sorbents, Weak Anion Exchange (WAX) exhibited the highest recovery rates among the sorbents evaluated (100.04% ± 2.74% recovery) via a dual adsorption mechanism combining amine-mediated ion exchange and hydrophobic interactions, achieving 30% higher efficiency than Hydrophile-Lipophile Balance (HLB) sorbents. The optimized method achieved low detection limits (LOD: 0.016–0.324 ng/L), high accuracy (spiked recovery: 80.12–115.76%) and precision (RSD < 10%). Method application revealed significantly lower PFAAs contamination in reservoir-sourced finished water compared to river/lake-derived supplies in the mid-Yangtze River region. This work effectively mitigates critical pretreatment biases in PFAAs analysis and provides a reliable solution to address the urgent need for monitoring PFAAs contamination in drinking water systems.

Graphic abstract

由于预处理过程中的非特异性吸附损失，饮用水中痕量全氟烷基酸（PFAAs）的准确检测仍然具有挑战性。本研究系统地评估了11种PFAAs在5种过滤膜和4种固相萃取（SPE）吸附剂上的吸附行为，以建立优化的分析方案。结果表明，玻璃纤维（GL）过滤器通过静电斥力将PFAAs的保留率降至最低（在水基中回收率为94.85%），优于聚丙烯（PP）等其他膜。在SPE吸附剂中，弱阴离子交换（WAX）通过胺离子交换和疏水相互作用的双重吸附机制，回收率最高（100.04%±2.74%），比亲水-亲脂平衡（HLB）吸附剂的回收率高30%。优化后的方法检出限低（LOD: 0.016 ~ 0.324 ng/L），准确度高（加标回收率：80.12 ~ 115.76%），精密度高（RSD）

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引用次数: 0

Determination of phenylphosphonic acid zinc salt in poly(lactic acid) using thermally assisted methylation pyrolysis–gas chromatography/mass spectrometry 热辅助甲基化热解-气相色谱/质谱法测定聚乳酸中苯基膦酸锌盐。

IF 2 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences

Pub Date : 2025-09-08 DOI: 10.1007/s44211-025-00845-2

Kazuko Matsui, Makoto Niwa, Atsushi Watanabe, Chuichi Watanabe, Norio Teramae

Biomass-based polymers such as poly(lactic acid) (PLA) have attracted much attention, because they are renewable, biocompatible, and nontoxic to the environment and have been used in various fields such as biomedical, agricultural, and food packaging industries. However, one of the common drawbacks of PLA-based materials is their low glass transition temperature in the amorphous state, while adding phenylphosphonic acid zinc salt (PPA-Zn) as a nucleating agent was found to be a promising method to improve the physical property of PLA. On the other hand, degradation of PLA-based materials in the environment may cause the pollution from the metal of a nucleating agent in PLA and quantification of nucleating agents in polymers is of interest. Polar phosphonic acid, a ligand in PPA-Zn, is not favorable for the analysis by gas chromatography/mass spectrometry (GC/MS). Such compounds containing polar groups are known to be effectively analyzed by GC/MS in combination with thermally assisted methylation reaction. In this study, we report on determination of PPA-Zn in PLA by coupling thermally assisted methylation with pyrolysis–GC/MS.

Graphical abstract

聚乳酸（PLA）等生物质基聚合物因其可再生、生物相容性好、对环境无毒等特点而受到广泛关注，在生物医学、农业、食品包装等领域得到了广泛应用。然而，PLA基材料的一个共同缺点是其非晶态玻璃化转变温度低，而添加苯基膦酸锌盐（PPA-Zn）作为成核剂被发现是一种很有前途的方法来改善PLA的物理性能。另一方面，聚乳酸基材料在环境中的降解可能会引起聚乳酸中成核剂的金属污染，聚合物中成核剂的量化是一个有趣的问题。极性膦酸是PPA-Zn中的配体，不利于气相色谱/质谱分析。这些含有极性基团的化合物已知可以通过GC/MS结合热辅助甲基化反应进行有效分析。在本研究中，我们报道了热辅助甲基化-热解-气相色谱/质谱联用法测定PLA中PPA-Zn。

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引用次数: 0

Advances in methods for rapid detection of Staphylococcus aureus and methicillin-resistant S. aureus 金黄色葡萄球菌和耐甲氧西林金黄色葡萄球菌快速检测方法研究进展。

IF 2 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences

Pub Date : 2025-09-06 DOI: 10.1007/s44211-025-00843-4

Linlin Zhuang, Mengling Zhu, Xinya Chen, Xiaoming Liu, Tianyu Yuan, Chuang Zhou, Xue Lian, Yunming Xu, Yu Zhang, Qiuping Shen

Staphylococcus aureus (S. aureus) and methicillin-resistant S. aureus (MRSA) are important pathogens that are closely associated with hospital-acquired and community-acquired infections. Rapid and accurate detection methods are important for early diagnosis and treatment of diseases. This paper reviews the latest research progress in the field of rapid detection of S. aureus and MRSA, including chromogenic media, immunological detection methods (e.g., enzyme-linked immunosorbent assays, lateral flow assays, colorimetric/fluorescent immunoassays, etc.), nucleic acid tests (including polymerase chain reaction-based methods, isothermal amplification techniques), biosensors, and novel emerging methods, etc. The advantages and disadvantages of these methods are systematically analyzed and discussed. Future research should focus on improving analytical sensitivity and specificity, shortening detection time, reducing detection cost, and promoting the integration of multiple platforms and intelligent analysis, especially for the development of technologies for point-of-care testing. These efforts are crucial to addressing the pressing need for rapid clinical diagnosis.

Graphical abstract

金黄色葡萄球菌（S. aureus）和耐甲氧西林金黄色葡萄球菌（MRSA）是与医院获得性和社区获得性感染密切相关的重要病原体。快速准确的检测方法对于疾病的早期诊断和治疗至关重要。本文综述了金黄色葡萄球菌和MRSA快速检测领域的最新研究进展，包括显色介质、免疫检测方法（如酶联免疫吸附法、侧流法、比色/荧光免疫法等）、核酸检测方法（包括基于聚合酶链反应的方法、等温扩增技术）、生物传感器和新兴方法等。对这些方法的优缺点进行了系统的分析和讨论。未来的研究应着眼于提高分析的灵敏度和特异性，缩短检测时间，降低检测成本，促进多平台的集成和智能分析，特别是开发护理点检测技术。这些努力对于解决快速临床诊断的迫切需要至关重要。

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引用次数: 0

In ovo sexing of chicken embryos using spectroscopic analysis 用光谱分析测定鸡胚胎的卵性别

IF 2 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences

Pub Date : 2025-09-03 DOI: 10.1007/s44211-025-00824-7

Mika Ishigaki

引用次数: 0

Investigation of experimental factors in in-capillary continuous enzyme assay to obtain plateau response by electrophoretically mediated microanalysis 毛细管内连续酶分析中电泳介导微量分析获得平台响应的实验因素研究。

IF 2 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences

Pub Date : 2025-08-28 DOI: 10.1007/s44211-025-00844-3

Toshio Takayanagi, Risa Okazaki, Karen Gotoda, Masanori Mine, Hitoshi Mizuguchi

In-capillary enzymatic reaction was investigated in electrophoretically mediated microanalysis to achieve plateau response of the reaction product. Hydrolysis of 4-nitrophenyl phosphate (NPP) with alkaline phosphatase (ALP) was used as a model enzymatic reaction. The reaction product of 4-nitrophenol (NP) was photometrically detected as a plateau response based on the continuous enzymatic reaction in the separation capillary and the ongoing electrophoretic resolution of the product from the reaction zone. Two types of injection were compared between an enzyme injection and a substrate injection, whereas the counterpart reagent was contained in the separation buffer. In both approaches, the plateau response of NP developed linearly with increasing sample injection period, as well as the enzyme concentration. At low separation voltages, NP accumulated at the reaction zone, and the plateau height was inversely proportional to the applied voltage. Comparing enzyme and substrate injection methods, a stable plateau response was more easily achieved with the enzyme injection. It is because the concentration of the substrate in the injected plug decreases during the electrophoresis, leading to a reduced reaction rate. In contract, enzyme injection maintains consistent reaction conditions. The Michaelis–Menten constant was determined with the plateau response by both injection modes, and the values were comparable with each other.

Graphical abstract

用电泳介导的微量分析方法研究了毛细管内酶促反应，获得了反应产物的平台响应。以碱性磷酸酶（ALP）水解4-硝基苯磷酸（NPP）为模型酶促反应。4-硝基苯酚（NP）的反应产物基于分离毛细管中连续的酶促反应和反应区中持续的电泳分辨，以平台响应的方式进行光度检测。两种类型的注射之间的酶注射和底物注射进行比较，而对应的试剂是包含在分离缓冲液。在这两种方法中，NP的平台响应随着样品注射周期和酶浓度的增加而线性发展。在低分离电压下，NP在反应区积累，平台高度与施加电压成反比。与底物注射法相比，酶注射法更容易获得稳定的平台响应。这是因为在电泳过程中，注入塞中的底物浓度降低，导致反应速率降低。在合同中，酶注射保持一致的反应条件。两种注射方式的Michaelis-Menten常数均与平台响应进行了比较，结果具有可比性。

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引用次数: 0

Review of methods for the determination of cellular cholesterol content: principles, advantages, limitations, applications, and perspectives 细胞胆固醇含量测定方法综述：原理、优点、局限性、应用和展望。

IF 2 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences

Pub Date : 2025-08-27 DOI: 10.1007/s44211-025-00842-5

Zihang Zhou, Jiangyu Zong, Ning Xu

Imbalances in cellular cholesterol homeostasis are associated with various diseases, and accurate determination of cholesterol levels and distribution is essential for a thorough understanding of cellular physiopathology. In this review, we comprehensively analyzed various techniques for cellular cholesterol determination. They include indirect methods based on SREBP2 activity monitoring, gas chromatography–liquid chromatography coupling, enzyme analysis, improved Abell–Kendall method, cholesterol-specific probes such as filipin III and cholesterol-dependent cytolysin, and cholesterol analogs such as dehydroergosterol, BODIPY-cholesterol, label-free as well as labeling Raman assays and mass spectrometry imaging techniques such as matrix-assisted laser desorption/ionization mass spectrometry imaging, desorption electrospray ionization mass spectrometry imaging, and nanoscale secondary ion mass spectrometry technology. Principles, advantages, and limitations of each technique are discussed in detail, their characteristics in terms of sensitivity, spatial resolution, and temporal resolution are compared in detail. Finally, suggestions for selecting the technique for different experimental objectives are also given. These findings will help researchers choose the most suitable method according to their own needs, provide strong support for cellular cholesterol research, and promote the development of related fields to better elucidate the significance of cellular cholesterol in normal biologic activities and also its intrinsic relationship with diseases.

Graphical abstract

细胞内胆固醇平衡失衡与多种疾病有关，准确测定胆固醇水平和分布对于彻底了解细胞生理病理至关重要。在这篇综述中，我们全面分析了细胞胆固醇测定的各种技术。它们包括基于SREBP2活性监测的间接方法，气相色谱-液相色谱耦合，酶分析，改进的abel- kendall方法，胆固醇特异性探针，如filipin III和胆固醇依赖性细胞溶解素，胆固醇类似物，如脱氢麦角甾醇，bodipy -胆固醇，无标记和标记拉曼分析和质谱成像技术，如基质辅助激光解吸/电离质谱成像。解吸电喷雾电离质谱成像，以及纳米级二次离子质谱技术。详细讨论了各种技术的原理、优点和局限性，比较了它们在灵敏度、空间分辨率和时间分辨率方面的特点。最后，针对不同的实验目标，给出了技术选择的建议。这些发现将有助于研究者根据自身需要选择最合适的方法，为细胞胆固醇研究提供有力支持，促进相关领域的发展，更好地阐明细胞胆固醇在正常生物活动中的意义及其与疾病的内在关系。

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引用次数: 0

Laser-induced acoustic desorption coupled with dielectric barrier discharge ionization mass spectrometry for analysis of thermally labile compounds 激光诱导声解吸耦合介质阻挡放电电离质谱分析热不稳定化合物。

IF 2 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences

Pub Date : 2025-08-22 DOI: 10.1007/s44211-025-00827-4

Xue Xiao, Dan Li, Xiandong Li, Pinchun Kang, Qiao Lu

Laser-induced acoustic desorption (LIAD) coupled with dielectric barrier discharge ionization mass spectrometry (DBDI-MS) has been developed as a novel technique for the direct detection of solid samples. LIAD is a “soft” desorption method that enables the volatilization of thermally labile and involatile compounds without significant degradation, while DBDI provides a gentle ionization process. Compared with laser desorption DBDI-MS (LD-DBDI-MS) or laser desorption ionization-MS (LDI-MS), this combination allows for the analysis of fragile molecules with minimal fragmentation. In this study, we optimized the LIAD-DBDI-MS system by adjusting key parameters such as the distance between the titanium foil and the ion source inlet, and the laser power density. The system was successfully employed for the analysis of melamine, copper phthalocyanine, and folic acid, demonstrating its capability to detect molecular species primarily as intact [M + H]⁺ or [M]⁻ ions with minimal or no fragmentation. The results highlight the potential of LIAD-DBDI-MS as a powerful tool for the analysis of complexes and fragile compounds in various fields, including pharmaceuticals and materials science.

Graphical abstract

激光诱导声解吸（LIAD）耦合介质阻挡放电电离质谱（DBDI-MS）是一种直接检测固体样品的新技术。LIAD是一种“软”解吸方法，可以使热不稳定和难合的化合物挥发而不会显着降解，而DBDI提供了一个温和的电离过程。与激光解吸DBDI-MS （LD-DBDI-MS）或激光解吸电离- ms （LDI-MS）相比，这种组合允许以最小的碎片分析脆弱分子。本研究通过调整钛箔与离子源入口的距离、激光功率密度等关键参数，对LIAD-DBDI-MS系统进行了优化。该系统成功地用于三聚氰胺、酞菁铜和叶酸的分析，证明了它能够检测完整的[M + H] +或[M]⁻+，几乎没有破裂。这些结果突出了LIAD-DBDI-MS在包括制药和材料科学在内的各个领域作为分析复合物和脆弱化合物的有力工具的潜力。

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引用次数: 0

Recent progress in functional materials for electrochemical detection of pesticides: modification strategies, performance, and future challenges 农药电化学检测功能材料的最新进展：改性策略、性能和未来挑战。

IF 2 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences

Pub Date : 2025-08-22 DOI: 10.1007/s44211-025-00838-1

Alvin Lim Teik Zheng, Ellie Yi Lih Teo, Yiu Pang Hung, Juniza Md Saad, Nor Hisam Zamakshshari, Eric Lim Teik Chung, Mohammed Abdillah Ahmad Farid, Yoshito Andou

Widespread pesticides use in agriculture has led to significant groundwater and food contamination, posing severe risks to both human health and the environment. Real-time detection of these emerging contaminants remains a formidable challenge. Electrochemical sensors and biosensors have remained a viable technique for overcoming the limits ascribed to their ultra-sensitivity and rapid detection times as well as excellent sensitivity, reliability, and selectivity. This comprehensive review will encompass the most recent advancements in the utilization of functional materials such as metal–organic framework, MXene, carbon organic framework, carbon nanotubes, and graphene modifiers for the detection of a myriad of pesticides via electrochemical detection techniques, electrode materials, electrolyte solutions, and sample matrices. This review compiles performance data, including detection limits and linear ranges, for various sensing platforms. Finally, the write-up will discuss advanced sensor technology and address the improvements made on electrochemical sensors and biosensors for pesticide detection, which will be important in future studies on pesticide determination.

Graphical abstract

农药在农业中的广泛使用导致地下水和食品受到严重污染，对人类健康和环境构成严重风险。实时检测这些新出现的污染物仍然是一项艰巨的挑战。电化学传感器和生物传感器仍然是一种可行的技术，可以克服其超灵敏度和快速检测时间的限制，以及出色的灵敏度、可靠性和选择性。这篇全面的综述将包括利用功能材料的最新进展，如金属有机框架、MXene、碳有机框架、碳纳米管和石墨烯改性剂，通过电化学检测技术、电极材料、电解质溶液和样品基质检测无数种农药。本综述汇编了各种传感平台的性能数据，包括检测限和线性范围。最后，本文将讨论先进的传感器技术，并讨论电化学传感器和生物传感器在农药检测方面的改进，这对未来农药检测的研究具有重要意义。

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引用次数: 0

Current status of detection strategies for pathogenic Escherichia coli: a brief review and summary 致病性大肠杆菌检测策略的现状：简要回顾与总结。

IF 2 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences

Pub Date : 2025-08-21 DOI: 10.1007/s44211-025-00836-3

Xudong Xue, Min He, Peng Wang, Yan Zhang

Pathogenic E. coli, a significant concern for researchers and health organizations worldwide, has caused numerous large-scale bacterial infection deaths worldwide. Low-dose infection and high pathogenicity underscore the need for rapid and accurate detection of E. coli to control its infection and protect human health and public health safety. This review outlines the main detection strategies for E. coli in recent years and divides them into six categories: traditional plate colony counting, immunology, optics, molecular biology, electrochemical sensing, and microchip technology. Recent representative articles on E. coli detection are also summarized and analyzed, and their advantages and disadvantages are discussed. Additionally, this review also presents the future direction and prospects of E. coli detection strategies. The main objective of this paper is to provide a comprehensive overview and constructive ideas to develop efficient, advanced, and immediate detection methods for E. coli and pathogenic microorganism detection, emphasizing the need for such strategies.

Graphical Abstract

致病性大肠杆菌是全世界研究人员和卫生组织关注的一个重大问题，它在世界范围内造成了许多大规模的细菌感染死亡。低剂量感染和高致病性强调需要快速准确地检测大肠杆菌，以控制其感染，保护人类健康和公共卫生安全。本文综述了近年来大肠杆菌的主要检测策略，并将其分为传统平板菌落计数、免疫学、光学、分子生物学、电化学传感和微芯片技术6大类。对近年来有关大肠杆菌检测的代表性文章进行了总结和分析，并对其优缺点进行了讨论。此外，本文还对大肠杆菌检测策略的发展方向和前景进行了展望。本文的主要目的是为开发高效、先进、快速的大肠杆菌和病原微生物检测方法提供全面的概述和建设性的意见，强调这些策略的必要性。

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引用次数: 0

Quantitative analysis using LA-ICP-MS and powder samples dispersed in polyvinyl alcohol films 用LA-ICP-MS和分散在聚乙烯醇薄膜中的粉末样品进行定量分析。

IF 2 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences

Pub Date : 2025-08-19 DOI: 10.1007/s44211-025-00835-4

Masahiro Kobayashi, Hiromasa Namiki, Hideo Hayashi

The combination of laser ablation and inductively coupled plasma mass spectrometry (LA-ICP-MS) is a powerful tool for direct analysis of solid samples. However, quantitative analyses by LA-ICP-MS require matrix-matched standard materials, which are not always available. In this study, we have developed a method that enables quantitative analyses of powder samples using LA-ICP-MS. The sample powder is dispersed in a polyvinyl alcohol (PVA) paste, which is a hydrophilic resin, and a film sample is obtained by a screen-printing technique. Analyte concentrations can be determined by a standard-addition method using an analyte-spiked film prepared by mixing standard solutions into the PVA paste. Because of its hydrophilicity, it is possible to increase the ratio of analytes (standard solutions) to the resin. Despite samples being diluted with the resin, this method has enough sensitivity to detect elements at concentrations < 0.1 μg/g. Several types of ceramic powder (SiC, ZrO₂, and TiO₂) were analyzed using this method, and all the analytes, the concentrations of which differed by five orders of magnitude (from < 1 μg/g to > 1 wt%), were accurately quantified. This method, in which reliable matrix-matched standard materials are easily tailored for each sample, can be potentially important in quantitative analyses using LA-ICP-MS.

Graphical abstract

激光烧蚀和电感耦合等离子体质谱（LA-ICP-MS）的结合是直接分析固体样品的有力工具。然而，LA-ICP-MS的定量分析需要基质匹配的标准材料，这并不总是可用的。在本研究中，我们开发了一种使用LA-ICP-MS对粉末样品进行定量分析的方法。将样品粉末分散在聚乙烯醇（PVA）浆料中，聚乙烯醇是一种亲水性树脂，通过丝网印刷技术获得薄膜样品。分析物浓度可以通过标准添加法测定，使用将标准溶液混合到PVA浆料中制备的分析物加标膜。由于其亲水性，可以增加分析物（标准溶液）与树脂的比例。尽管样品被树脂稀释，但该方法具有足够的灵敏度，可以检测浓度为2的元素，并使用该方法分析了TiO2)，并且所有浓度相差5个数量级（从1 wt%开始）的分析物都能准确定量。这种方法可以很容易地为每个样品定制可靠的基质匹配标准材料，在使用LA-ICP-MS进行定量分析中具有潜在的重要意义。

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引用次数: 0