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Insect Meal (Tenebrio molitor) Has High Nutrient Digestibility for Newly Weaned Piglets
IF 1.7 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2025-02-19 DOI: 10.1111/asj.70036
Juliana Carolina Pereira, Alan Roberto Alves da Silva, Bernardetti Cajaíba Oliveira Rossiti, Rodrigues da Silva, Letícia Garbin Ribeiro da Silva, Mariane Zabotto Evangelista, Urbano dos Santos Ruiz, Marcos Livio Panhoza Tse

This study aimed to evaluate the inclusion of insect larvae meal (Tenebrio molitor) on the apparent total digestibility of dry matter (DM), gross energy (GE), ether extract (EE), crude protein (CP), neutral and acid detergent fiber (NDF and FDA), and the apparent and standardized ileal digestibility of amino acids (Exp.1) and to evaluate the inclusion of insect meal with iron and manganese supplementation on growth performance and serum hemoglobin concentration by collecting blood (day zero), on the 14th and 32nd days of the experiment (Exp.2). The standardized ileal digestibility values for amino acids and crude protein of insect meal were similar to those found for soybean meal, with high metabolizable energy values (Exp.1), and there was no difference in performance for any of the variables analyzed (p ≥ 0.05) in any of the periods (Exp.2). For serum hemoglobin values, there was no interaction (p ≥ 0.05) between the treatments used and the collection days; however, hemoglobin values increased (p < 0.05) at each collection time. It can therefore be concluded that insect meal for pigs can be considered an alternative ingredient to soybean meal as a source of protein in piglet nutrition due to high digestibility of amino acids without deleterious effect on productive performance.

{"title":"Insect Meal (Tenebrio molitor) Has High Nutrient Digestibility for Newly Weaned Piglets","authors":"Juliana Carolina Pereira,&nbsp;Alan Roberto Alves da Silva,&nbsp;Bernardetti Cajaíba Oliveira Rossiti,&nbsp;Rodrigues da Silva,&nbsp;Letícia Garbin Ribeiro da Silva,&nbsp;Mariane Zabotto Evangelista,&nbsp;Urbano dos Santos Ruiz,&nbsp;Marcos Livio Panhoza Tse","doi":"10.1111/asj.70036","DOIUrl":"https://doi.org/10.1111/asj.70036","url":null,"abstract":"<div>\u0000 \u0000 <p>This study aimed to evaluate the inclusion of insect larvae meal (<i>Tenebrio molitor</i>) on the apparent total digestibility of dry matter (DM), gross energy (GE), ether extract (EE), crude protein (CP), neutral and acid detergent fiber (NDF and FDA), and the apparent and standardized ileal digestibility of amino acids (Exp.1) and to evaluate the inclusion of insect meal with iron and manganese supplementation on growth performance and serum hemoglobin concentration by collecting blood (day zero), on the 14th and 32nd days of the experiment (Exp.2). The standardized ileal digestibility values for amino acids and crude protein of insect meal were similar to those found for soybean meal, with high metabolizable energy values (Exp.1), and there was no difference in performance for any of the variables analyzed (<i>p</i> ≥ 0.05) in any of the periods (Exp.2). For serum hemoglobin values, there was no interaction (<i>p</i> ≥ 0.05) between the treatments used and the collection days; however, hemoglobin values increased (<i>p</i> &lt; 0.05) at each collection time. It can therefore be concluded that insect meal for pigs can be considered an alternative ingredient to soybean meal as a source of protein in piglet nutrition due to high digestibility of amino acids without deleterious effect on productive performance.</p>\u0000 </div>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143447059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of Pomegranate Flower Extracts on Antioxidant Properties, Phenolic Content, and Quality Attributes of Nitrite Reduced Chicken Sausages
IF 1.7 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2025-02-13 DOI: 10.1111/asj.70039
Cem Okan Özer, Ganime Beyzanur Var, Ezgi Demir Özer, Birol Kiliç

The objective of present study was to examine the potential use of pomegranate flower extracts (PFEs) obtained from water and ethanol at antimicrobial doses in nitrite-reduced chicken sausages. The impact of the extracts on the physicochemical, textural, and sensory attributes of the sausages was evaluated using both reduced doses of nitrite and a nitrite-free preparation. The results revealed that incorporating PFE resulted in a sixfold and twofold enhancement in total phenolic content and antioxidant activity, respectively. The DPPH radical scavenging activity in sausages produced with ethanol-based PFE was more than twice as high as in sausages treated with sodium nitrite. Additionally, the combination of low-dose nitrite with PFE showed a reduction in TBARS values, leading to the prevention of lipid oxidation. Sausages with PFE exhibited lower pH and redness values, while maintaining similar aroma and odor characteristics as the controls. Although there was a slight negative impact on texture, color, and overall acceptability, PFE can be considered a potential alternative for improving bioactive content and oxidative stability of low-nitrite chicken sausages. The application of PFE could offer a promising opportunity to enhance the nutritional profile and quality attributes of processed meat products.

{"title":"Effects of Pomegranate Flower Extracts on Antioxidant Properties, Phenolic Content, and Quality Attributes of Nitrite Reduced Chicken Sausages","authors":"Cem Okan Özer,&nbsp;Ganime Beyzanur Var,&nbsp;Ezgi Demir Özer,&nbsp;Birol Kiliç","doi":"10.1111/asj.70039","DOIUrl":"https://doi.org/10.1111/asj.70039","url":null,"abstract":"<p>The objective of present study was to examine the potential use of pomegranate flower extracts (PFEs) obtained from water and ethanol at antimicrobial doses in nitrite-reduced chicken sausages. The impact of the extracts on the physicochemical, textural, and sensory attributes of the sausages was evaluated using both reduced doses of nitrite and a nitrite-free preparation. The results revealed that incorporating PFE resulted in a sixfold and twofold enhancement in total phenolic content and antioxidant activity, respectively. The DPPH radical scavenging activity in sausages produced with ethanol-based PFE was more than twice as high as in sausages treated with sodium nitrite. Additionally, the combination of low-dose nitrite with PFE showed a reduction in TBARS values, leading to the prevention of lipid oxidation. Sausages with PFE exhibited lower pH and redness values, while maintaining similar aroma and odor characteristics as the controls. Although there was a slight negative impact on texture, color, and overall acceptability, PFE can be considered a potential alternative for improving bioactive content and oxidative stability of low-nitrite chicken sausages. The application of PFE could offer a promising opportunity to enhance the nutritional profile and quality attributes of processed meat products.</p>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/asj.70039","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143404672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Production and Utilization of Fermented Rice Bran as Animal Feed
IF 1.7 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2025-02-13 DOI: 10.1111/asj.70037
Jamal James D. Manlapig, Hiroki Matsui

Rice bran, a byproduct of milling rice (Oryza sativa), is widely used in Asian preserved foods and the feed industry. Rich in carbohydrates, protein, fat, and various bioactive compounds, it offers significant growth and health benefits. It is commonly used as a replacement for cereals and grains as an energy source in the diet. However, its potential as animal feed is constrained by antinutritional factors and susceptibility to rancidity due to lipase activity. Recently, there has been a growing interest in fermentation to enhance rice bran's nutritional profile. It has been confirmed that fermenting rice bran could increase the proportion of crude protein and fat. Moreover, feeding fermented rice bran (FRB) improves the growth performance of food-producing animals by increasing efficiency. These results, along with their beneficial effect on gut microbiota, may improve the overall health of food animals. In this review, we aimed to summarize the latest research on the fermentation of rice bran, focusing on the role of microorganisms and the factors influencing the quality and nutritional value of FRB. Additionally, we will explore the impact of FRB on nutrient composition and animal performance, highlighting its potential as a valuable feed ingredient.

{"title":"Production and Utilization of Fermented Rice Bran as Animal Feed","authors":"Jamal James D. Manlapig,&nbsp;Hiroki Matsui","doi":"10.1111/asj.70037","DOIUrl":"https://doi.org/10.1111/asj.70037","url":null,"abstract":"<p>Rice bran, a byproduct of milling rice (<i>Oryza sativa</i>), is widely used in Asian preserved foods and the feed industry. Rich in carbohydrates, protein, fat, and various bioactive compounds, it offers significant growth and health benefits. It is commonly used as a replacement for cereals and grains as an energy source in the diet. However, its potential as animal feed is constrained by antinutritional factors and susceptibility to rancidity due to lipase activity. Recently, there has been a growing interest in fermentation to enhance rice bran's nutritional profile. It has been confirmed that fermenting rice bran could increase the proportion of crude protein and fat. Moreover, feeding fermented rice bran (FRB) improves the growth performance of food-producing animals by increasing efficiency. These results, along with their beneficial effect on gut microbiota, may improve the overall health of food animals. In this review, we aimed to summarize the latest research on the fermentation of rice bran, focusing on the role of microorganisms and the factors influencing the quality and nutritional value of FRB. Additionally, we will explore the impact of FRB on nutrient composition and animal performance, highlighting its potential as a valuable feed ingredient.</p>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/asj.70037","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143404671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of Dietary Replacement of Alfalfa Hay With Corn Silage on Energy Utilization Efficiency and Metabolizable Energy Requirements in Crossbred Hu Ewes During Early Lactation
IF 1.7 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2025-02-13 DOI: 10.1111/asj.70034
Qing Qing, Mitsuru Tsubo, Wuchen Du, Atsushi Tsunekawa, Fujiang Hou, Toshiyoshi Ichinohe

We evaluated the effect of dietary substituting corn silage (CS) for alfalfa hay (AH) on feed intake, lactation performance, and energy utilization efficiency of crossbred Hu ewes. In a completely randomized experiment, 12 lactating crossbred ewes (3 ± 1.5 DIM) were fed one of three isoenergetic diets with forage-to-concentrate ratio of 70:30. The three experimental diets (CON, CS1, and CS2) were formulated by replacing AH with different levels of CS (0%, 30%, and 60%, respectively). Energy balance was determined through a metabolism and respiration trial. We observed identical trends for feed intake and gross energy intake (GEI); the GEI ranged from 0.97 to 1.34 (MJ/kg BW0.75 day) among the treatments. Around 26%–33% of the GEI was lost as feces, 56%–60% for heat production, 14%–16% for milk production, 5%–6% for methane emission, and 3% for urinary energy. The metabolizable energy requirement for maintenance of crossbred Hu ewe was estimated to be 484.7 kJ/kg body weight0.75/day. Furthermore, the energy digestibility and metabolizability of the CS diets were significantly greater than those of the CON diet. Substituting AH for up to 60% CS increased energy utilization efficiency without detrimental effects on milk production in crossbred Hu ewes fed a forage-based diet.

{"title":"Effects of Dietary Replacement of Alfalfa Hay With Corn Silage on Energy Utilization Efficiency and Metabolizable Energy Requirements in Crossbred Hu Ewes During Early Lactation","authors":"Qing Qing,&nbsp;Mitsuru Tsubo,&nbsp;Wuchen Du,&nbsp;Atsushi Tsunekawa,&nbsp;Fujiang Hou,&nbsp;Toshiyoshi Ichinohe","doi":"10.1111/asj.70034","DOIUrl":"https://doi.org/10.1111/asj.70034","url":null,"abstract":"<div>\u0000 \u0000 <p>We evaluated the effect of dietary substituting corn silage (CS) for alfalfa hay (AH) on feed intake, lactation performance, and energy utilization efficiency of crossbred Hu ewes. In a completely randomized experiment, 12 lactating crossbred ewes (3 ± 1.5 DIM) were fed one of three isoenergetic diets with forage-to-concentrate ratio of 70:30. The three experimental diets (CON, CS1, and CS2) were formulated by replacing AH with different levels of CS (0%, 30%, and 60%, respectively). Energy balance was determined through a metabolism and respiration trial. We observed identical trends for feed intake and gross energy intake (GEI); the GEI ranged from 0.97 to 1.34 (MJ/kg BW<sup>0.75</sup> day) among the treatments. Around 26%–33% of the GEI was lost as feces, 56%–60% for heat production, 14%–16% for milk production, 5%–6% for methane emission, and 3% for urinary energy. The metabolizable energy requirement for maintenance of crossbred Hu ewe was estimated to be 484.7 kJ/kg body weight<sup>0.75</sup>/day. Furthermore, the energy digestibility and metabolizability of the CS diets were significantly greater than those of the CON diet. Substituting AH for up to 60% CS increased energy utilization efficiency without detrimental effects on milk production in crossbred Hu ewes fed a forage-based diet.</p>\u0000 </div>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143404670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ursodeoxycholic Acid Alleviates Palmitic Acid–Induced Apoptosis in Bovine Mammary Epithelial Cells
IF 1.7 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2025-02-10 DOI: 10.1111/asj.70038
Md. Rezwanul Habib, Yukako Tokutake, Shinichi Yonekura

This study investigated the protective effects of ursodeoxycholic acid (UDCA) against PA-induced apoptosis in the MAC-T bovine mammary epithelial cell (bMEC) line by assessing the level of cell viability, oxidative stress indicators, and expression of endoplasmic reticulum (ER) stress markers. MAC-T cells were pretreated with UDCA at 25, 50, and 100 μM before exposure to PA at 300 μM. UDCA was noncytotoxic at these concentrations and significantly improved cell viability, which was reduced by PA. UDCA pretreatment notably decreased PA-induced expression of GRP78, XBP1s, ATF4, and CHOP mRNA, indicating reduced ER stress. Moreover, UDCA substantially lowered PA-induced reactive oxygen species production and dichlorofluorescein fluorescence intensities. Although PA treatment elevated GSSG levels and disrupted redox balance by decreasing both the total GSH/GSSG and reduced GSH/GSSG ratios, UDCA effectively counteracted these effects. Specifically, UDCA reduced PA-induced GSSG levels and restored redox balance by increasing both total and reduced GSH/GSSG ratios. These findings suggest that UDCA mitigates PA-induced apoptosis in MAC-T cells by enhancing redox homeostasis. Therefore, incorporating UDCA into dairy cow feed could help reduce PA-induced cellular stress and improve milk production during periods of negative energy balance.

{"title":"Ursodeoxycholic Acid Alleviates Palmitic Acid–Induced Apoptosis in Bovine Mammary Epithelial Cells","authors":"Md. Rezwanul Habib,&nbsp;Yukako Tokutake,&nbsp;Shinichi Yonekura","doi":"10.1111/asj.70038","DOIUrl":"https://doi.org/10.1111/asj.70038","url":null,"abstract":"<p>This study investigated the protective effects of ursodeoxycholic acid (UDCA) against PA-induced apoptosis in the MAC-T bovine mammary epithelial cell (bMEC) line by assessing the level of cell viability, oxidative stress indicators, and expression of endoplasmic reticulum (ER) stress markers. MAC-T cells were pretreated with UDCA at 25, 50, and 100 μM before exposure to PA at 300 μM. UDCA was noncytotoxic at these concentrations and significantly improved cell viability, which was reduced by PA. UDCA pretreatment notably decreased PA-induced expression of GRP78, XBP1s, ATF4, and CHOP mRNA, indicating reduced ER stress. Moreover, UDCA substantially lowered PA-induced reactive oxygen species production and dichlorofluorescein fluorescence intensities. Although PA treatment elevated GSSG levels and disrupted redox balance by decreasing both the total GSH/GSSG and reduced GSH/GSSG ratios, UDCA effectively counteracted these effects. Specifically, UDCA reduced PA-induced GSSG levels and restored redox balance by increasing both total and reduced GSH/GSSG ratios. These findings suggest that UDCA mitigates PA-induced apoptosis in MAC-T cells by enhancing redox homeostasis. Therefore, incorporating UDCA into dairy cow feed could help reduce PA-induced cellular stress and improve milk production during periods of negative energy balance.</p>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/asj.70038","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143380378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Curcumin Suppresses ROS Production and Increases Mitochondrial Activity in Cumulus Cells and Oocytes of COCs Derived From Non-Vascularized Follicles in Pigs
IF 1.7 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2025-02-05 DOI: 10.1111/asj.70032
Tomoya Nakanishi, Shingo Tonai, Haruto Ichikawa, Shota Mori, Shinji Ishihara, Yongjin Chang, Yasuhisa Yamashita

In vitro maturation (IVM) produces offspring from domestic animals; however, the blastocyst rate after IVM was low. We previously reported that the developmental competence of oocytes derived from follicles with blood vessels absent on the surface (non-vascularized follicles: NVF) is quite low compared to those derived from follicles with blood vessels present on the surface (vascularized follicles: VF). Thus, it is important to develop technique to improve the quality of NVF-derived oocyte by IVM. Since it has been reported that reactive oxygen species (ROS) reduces oocyte quality, in this study, we investigated whether curcumin that is known as antioxidant could improve oocyte quality derived from NVF. As results, cultivation of NVF Cumulus-oocyte complexes (COCs) with curcumin significantly improved cumulus expansion and oocyte meiotic maturation of NVF COCs compared to those of NVF COCs without curcumin. Cultivation with curcumin of NVF COCs significantly improved the proliferative activity of cumulus cells. Furthermore, the cultivation significantly reduced ROS activity and increased mitochondrial activity. Hence, it was revealed that the addition of curcumin to the maturation medium increased mitochondrial activity and reduced ROS levels in NVF-derived cumulus cells and oocytes, thereby improving the maturation of oocytes within COCs.

{"title":"Curcumin Suppresses ROS Production and Increases Mitochondrial Activity in Cumulus Cells and Oocytes of COCs Derived From Non-Vascularized Follicles in Pigs","authors":"Tomoya Nakanishi,&nbsp;Shingo Tonai,&nbsp;Haruto Ichikawa,&nbsp;Shota Mori,&nbsp;Shinji Ishihara,&nbsp;Yongjin Chang,&nbsp;Yasuhisa Yamashita","doi":"10.1111/asj.70032","DOIUrl":"https://doi.org/10.1111/asj.70032","url":null,"abstract":"<div>\u0000 \u0000 <p>In vitro maturation (IVM) produces offspring from domestic animals; however, the blastocyst rate after IVM was low. We previously reported that the developmental competence of oocytes derived from follicles with blood vessels absent on the surface (non-vascularized follicles: NVF) is quite low compared to those derived from follicles with blood vessels present on the surface (vascularized follicles: VF). Thus, it is important to develop technique to improve the quality of NVF-derived oocyte by IVM. Since it has been reported that reactive oxygen species (ROS) reduces oocyte quality, in this study, we investigated whether curcumin that is known as antioxidant could improve oocyte quality derived from NVF. As results, cultivation of NVF Cumulus-oocyte complexes (COCs) with curcumin significantly improved cumulus expansion and oocyte meiotic maturation of NVF COCs compared to those of NVF COCs without curcumin. Cultivation with curcumin of NVF COCs significantly improved the proliferative activity of cumulus cells. Furthermore, the cultivation significantly reduced ROS activity and increased mitochondrial activity. Hence, it was revealed that the addition of curcumin to the maturation medium increased mitochondrial activity and reduced ROS levels in NVF-derived cumulus cells and oocytes, thereby improving the maturation of oocytes within COCs.</p>\u0000 </div>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143112144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dietary Protein Reduction in Lean Pigs From Pietrain Paternal Line Under Commercial Setting: An Interplay Between Nitrogen Efficiency and Meat Attributes
IF 1.7 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2025-02-02 DOI: 10.1111/asj.70033
Javier Álvarez-Rodríguez, Alba Vigo-Morancho, Francisco Javier Miana-Mena, Leticia Pérez-Ciria, Maria Angeles Latorre

A trial was conducted to research the impact of reducing dietary crude protein (CP) levels from 14.8% to 12.6% in the finishing diet (90–120 kg of body weight) of lean pigs, with an essential AA supply (8.8 g Lys, 2.1 g Met, 6.7 g Thr, 5.5 g Ile, and 6.7 g Val, per kilogram of feed) in intact males and females under commercial setting. The productive performances and nitrogen (N) balance, blood markers related to protein metabolism, apparent digestibility of nutrients, carcass, and meat quality were evaluated. This dietary CP restriction did not impair the growth performances, decreased the N excretion, and did not affect the apparent digestibility of most nutrients, except phosphorus digestibility, which was reduced in the low-CP diet. The responses to this dietary CP restriction were independent of sex, except the serum urea concentration and total proteins, which were only steadily reduced in intact males fed the low-CP diet. The low-CP diet reduced the nutritional pork quality by increasing the moisture content, although the technological characteristics of meat were not affected. Further studies are required to improve the sex-tailored feeding strategies for high-conformed lean pigs in an environmentally friendly context.

{"title":"Dietary Protein Reduction in Lean Pigs From Pietrain Paternal Line Under Commercial Setting: An Interplay Between Nitrogen Efficiency and Meat Attributes","authors":"Javier Álvarez-Rodríguez,&nbsp;Alba Vigo-Morancho,&nbsp;Francisco Javier Miana-Mena,&nbsp;Leticia Pérez-Ciria,&nbsp;Maria Angeles Latorre","doi":"10.1111/asj.70033","DOIUrl":"10.1111/asj.70033","url":null,"abstract":"<div>\u0000 \u0000 <p>A trial was conducted to research the impact of reducing dietary crude protein (CP) levels from 14.8% to 12.6% in the finishing diet (90–120 kg of body weight) of lean pigs, with an essential AA supply (8.8 g Lys, 2.1 g Met, 6.7 g Thr, 5.5 g Ile, and 6.7 g Val, per kilogram of feed) in intact males and females under commercial setting. The productive performances and nitrogen (N) balance, blood markers related to protein metabolism, apparent digestibility of nutrients, carcass, and meat quality were evaluated. This dietary CP restriction did not impair the growth performances, decreased the N excretion, and did not affect the apparent digestibility of most nutrients, except phosphorus digestibility, which was reduced in the low-CP diet. The responses to this dietary CP restriction were independent of sex, except the serum urea concentration and total proteins, which were only steadily reduced in intact males fed the low-CP diet. The low-CP diet reduced the nutritional pork quality by increasing the moisture content, although the technological characteristics of meat were not affected. Further studies are required to improve the sex-tailored feeding strategies for high-conformed lean pigs in an environmentally friendly context.</p>\u0000 </div>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Acknowledgment to Reviewers
IF 1.7 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2025-01-31 DOI: 10.1111/asj.70035
{"title":"Acknowledgment to Reviewers","authors":"","doi":"10.1111/asj.70035","DOIUrl":"https://doi.org/10.1111/asj.70035","url":null,"abstract":"","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"96 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143121269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Heat stress induces oxidative stress and activates the KEAP1-NFE2L2-ARE pathway in reproduction-related cells
IF 1.7 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2025-01-26 DOI: 10.1111/asj.70023
Hanako Bai, Manabu Kawahara, Kazuya Kusama, Toshihiro Sakurai, Christiane Pfarrer, Masashi Takahashi

Heat stress negatively affects the reproductive function of in animals and humans. Although a relationship between heat and oxidative stress has been suggested, the underlying mechanism has not been sufficiently examined in reproduction-related cells. Therefore, we aimed to investigate whether heat stress induces oxidative stress using a variety of reproduction-related cells including bovine placental and cumulus–granulosa cells, human cell lines derived from cervical and endometrial cancers, and fibroblasts derived from endometrium. Quantitative polymerase chain reaction analysis showed that the expression levels of representative heat and oxidative stress–related genes were significantly increased in cells cultured at high temperatures compared with those in cells cultured at basal temperatures. Moreover, luciferase reporter assays showed that the reporter activity of the heat shock element and antioxidant responsive element (ARE) was increased in cells cultured at high temperatures compared with that in cells cultured at basal temperatures. Furthermore, the stability of nuclear factor erythroid 2 like 2 (NFE2L2), a master regulator of the cellular stress response, increased under high temperatures. Point mutations in Kelch-like ECH-associated protein 1 (KEAP1) cysteine residues reduced the luciferase activity. Our results suggest that heat stress induces oxidative stress and that the KEAP1-NFE2L2-ARE pathway may play a protective role in reproduction-related cells against heat stress.

热应激会对动物和人类的生殖功能产生负面影响。虽然有人认为热与氧化应激之间存在关系,但尚未在生殖相关细胞中对其基本机制进行充分研究。因此,我们利用多种生殖相关细胞,包括牛胎盘细胞和积液-颗粒细胞、从宫颈癌和子宫内膜癌中提取的人类细胞系以及从子宫内膜提取的成纤维细胞,研究热应激是否会诱导氧化应激。定量聚合酶链反应分析表明,与在基础温度下培养的细胞相比,在高温下培养的细胞中与热和氧化应激有关的代表性基因的表达水平明显增加。此外,荧光素酶报告实验表明,高温培养的细胞与基础温度培养的细胞相比,热休克元件和抗氧化反应元件(ARE)的报告活性有所增加。此外,细胞应激反应的主调控因子红细胞核因子 2 like 2(NFE2L2)的稳定性在高温下也有所增加。Kelch-like ECH-associated protein 1(KEAP1)半胱氨酸残基的点突变降低了荧光素酶的活性。我们的研究结果表明,热应激会诱导氧化应激,而KEAP1-NFE2L2-ARE通路可能在生殖相关细胞中对热应激起到保护作用。
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引用次数: 0
Physicochemical Properties and Contents of Amino Acids, Fatty Acids, and Phytanic Acid in Goat Meat Produced by Grazing System in an Abandoned Rice Field
IF 1.7 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2025-01-26 DOI: 10.1111/asj.70028
Nthanda Chimwemwe Amini, Taketo Obitsu, Toshihisa Sugino, Shogo Shinde

This study evaluated carcass weight and physicochemical properties of loin and leg meat parts of Saanen castrated goats (n = 4) reared by grazing for 5 months in an abandoned rice field with concentrate supplements, comparing with meat of goats (n = 4) reared by confined feeding with grass silage and concentrate. Carcass and meat part weights of goats in the grazing group were lower (p < 0.01) than confined group. Shear force and cooking loss of meat showed no differences between the feeding groups. For meat color, L* value of loin meat tended to be lower (p < 0.15) for the grazing group compared with the confined group. Moisture content tended to be higher, but fat content tended to be lower (p < 0.15) in loin meat for the grazing group. Carnosine and alanine contents in loin meat were higher (p < 0.05) for the grazing group. Polyunsaturated fatty acid composition in meat was higher (p < 0.05), whereas monounsaturated fatty acid composition was lower (p < 0.05) for the grazing group. Phytanic acid content in both meat parts showed higher value (p < 0.05) for the grazing group. These results indicate that goat meat produced by grazing system in an abandoned rice field contains beneficial compounds for human health.

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引用次数: 0
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Animal Science Journal
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