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Impact of Genome-Wide and Regional Inbreeding on Semen Production Traits in Beef and Dairy Bulls 全基因组和区域近交对肉牛和奶牛精液生产性状的影响。
IF 1.2 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2025-12-03 DOI: 10.1111/asj.70138
Rintaro Nagai, Masashi Kinukawa, Toshio Watanabe, Atsushi Ogino, Kazuhito Kurogi, Kazunori Adachi, Yoshinobu Uemoto

To evaluate inbreeding depression, which is a reduction in the phenotypic mean of a population due to mating between close relatives, for semen production traits in cattle, it is necessary to understand inbreeding depression both regionally and across the genome. This study aimed to investigate genome-wide and regional inbreeding depression in semen production traits in Japanese Black bulls (JB) and Holstein bulls (HOL). The present study used 615 JB and 873 HOL with a 50K single-nucleotide polymorphism (SNP) BeadChip and five semen production traits. First, we estimated the regression coefficients of the semen production traits on pedigree-based and genomic inbreeding coefficients. Our results indicated significant negative effects of inbreeding on four semen production traits in JB, whereas only one trait was significantly affected in HOL. Second, we performed genome-wide association studies to identify run of homozygosity (ROH) regions that had an unfavorable effect on the traits. Our results showed no significant SNPs in JB; however, SNPs on chromosomes 7 and 17 were significantly associated with sperm quality in HOL. This study revealed that semen production traits are influenced by genome-wide and regional inbreeding depression in JB and HOL, although the effects vary depending on the target population.

为了评估近交抑制(由于近亲交配导致种群表型平均值的降低)对牛精液生产性状的影响,有必要了解区域和整个基因组的近交抑制。本研究旨在研究日本黑公牛(JB)和荷斯坦公牛(HOL)的全基因组和区域近交抑制的产精性状。本研究以615株JB和873株HOL为研究对象,采用50K单核苷酸多态性(SNP) BeadChip和5个产精性状。首先,我们估算了产精性状对家系近交系数和基因组近交系数的回归系数。结果表明,近交对JB的4个产精性状有显著的负向影响,而对HOL只有1个性状有显著的负向影响。其次,我们进行了全基因组关联研究,以确定对性状有不利影响的纯合性(ROH)区域的运行。我们的结果显示,在JB中没有明显的snp;然而,在HOL中,染色体7和17上的snp与精子质量显著相关。本研究表明,精子生产性状受到JB和HOL的全基因组和区域近交抑制的影响,尽管其影响因目标群体而异。
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引用次数: 0
Effects of Fermented Total Mixed Ration on Feed Intake, Digestibility, Rumen Fermentation, and Milk Production in Dairy Cows in a Hot Environment 发酵总混合日粮对高温环境下奶牛采食量、消化率、瘤胃发酵和产奶量的影响
IF 1.2 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2025-12-01 DOI: 10.1111/asj.70116
Keiko Nishimura, Fuminori Terada

This study aimed to evaluate the effects of a fermented total mixed ration (TMR) on feed intake, digestibility, rumen fermentation, and milk production in dairy cows in a hot environment. The experiment was conducted twice, with four multiparous Holstein cows used in each trial. Fresh and fermented TMR were used as dietary treatments in a crossover experimental design. Fresh and fermented TMR had the same diet composition. Fresh TMR was prepared every morning. Fermented TMR was prepared by baling fresh TMR and sealing it with a bale wrapper, followed by storage outdoors. Dry matter intake and digestibility did not differ between groups. Cows fed fermented TMR showed increased intake and digestibility of crude protein and non-fiber carbohydrate. Rumen total volatile fatty acids and propionate concentration were higher in cows fed fermented TMR than in those fed fresh TMR. Milk yield tended to increase in the cows fed fermented TMR. These results indicated that feeding fermented TMR to dairy cows in a hot environment altered feed digestibility and rumen fermentation and increased milk production. Therefore, fermented TMR is a suitable feed for lactating cows in a hot environment and is an effective technology for heat management.

本试验旨在评价热环境下发酵总混合日粮(TMR)对奶牛采食量、消化率、瘤胃发酵和产奶量的影响。试验分两次进行,每次选用4头产荷斯坦奶牛。在交叉试验设计中,新鲜TMR和发酵TMR作为饲粮处理。鲜TMR和发酵TMR的日粮组成相同。每天早上准备新鲜的TMR。发酵TMR是将新鲜TMR打包,用包捆包装密封,然后在室外储存。干物质采食量和消化率各组间无显著差异。饲喂发酵TMR的奶牛对粗蛋白质和非纤维性碳水化合物的采食量和消化率均有所提高。发酵TMR组瘤胃总挥发性脂肪酸和丙酸浓度高于新鲜TMR组。饲喂发酵TMR的奶牛产奶量有增加的趋势。由此可见,在高温环境下饲喂发酵TMR可改变奶牛饲料消化率和瘤胃发酵,提高产奶量。因此,发酵TMR是热环境下泌乳奶牛的适宜饲料,是一种有效的热管理技术。
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引用次数: 0
The Effect of Different Dietary Protein Sources and Tannins on the Fattening Performance and Carcass Characteristics of Finishing Holstein–Friesian Bulls 饲粮不同蛋白质来源和单宁对育肥牛增肥性能和胴体特性的影响。
IF 1.2 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2025-11-30 DOI: 10.1111/asj.70132
Zenon Nogalski, Martyna Momot, Monika Sobczuk-Szul

The aim of this study was to evaluate the effect of different protein sources on the fattening performance and carcass characteristics of finishing bulls. Thirty HF bulls in three feeding groups were fed diets with different protein sources: rapeseed meal (RSM), Vicia faba seeds (VFB), and lupine seeds (LP). The proportions (g/kg DM) of feed ingredients in the diets were as follows: group RSM-MS (maize silage) 620, TG (Triticale grain) 210, and RSM 150; group VFB-MS 620, TG 170, and VFB 190; group LP-MS 620, TG 190, and LP 170. Bulls fed RSM were characterized by the highest ADG and feed intake, and the most desirable FCR (p < 0.05). Carcass conformation and fatness scores were higher in group RSM than in groups VFB and LP. Tannins contained in V. faba seeds did not exert a positive influence on feed intake, average daily gain (ADG) or the feed conversion ratio (FCR). The value of WBSF was lowest in group VFB, indicating superior meat tenderness (p < 0.05). The inclusion of feed component containing tannins resulted in lower (p < 0.05) redness and higher pH stability of their meat during storage.

本研究旨在探讨不同蛋白质来源对育肥牛增肥性能和胴体性状的影响。3个饲喂组30头HF公牛分别饲喂不同蛋白质来源的饲粮:油菜籽粕(RSM)、蚕豆籽(VFB)和羽扇豆籽(LP)。饲粮中饲料成分的比例(g/kg DM)为:RSM- ms(玉米青贮)620,TG(小黑麦籽粒)210,RSM 150;组VFB- ms 620, TG 170, VFB 190;组LP- ms 620, TG 190, LP 170。饲喂RSM的公牛平均日增重和采食量最高,料重比最高(p
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引用次数: 0
Plastic Flooring as a Bedding Alternative: A Welfare Trade-Off for Broiler Chickens 塑料地板作为床上用品的替代品:肉鸡的福利权衡。
IF 1.2 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2025-11-30 DOI: 10.1111/asj.70133
Bruna Barreto Przybulinski, Maria Fernanda de Castro Burbarelli, Felipe Cardoso Serpa, Irenilza de Alencar Naas, Jean Kaique Valentim, Claudia Marie Komiyama, Fabiana Ribeiro Caldara, Rodrigo Garófallo Garcia

This study evaluated the behavior and incidence of locomotor disorders in broiler chickens raised on plastic flooring (PF) with and without a nanotechnological antimicrobial additive compared with a conventional system using wood shavings. A total of 1500 male Ross 408 lineage broilers were used, arranged in a completely randomized design with five treatments, wood shavings (WS), PF, PF + WS, plastic flooring with additive (PFA), and PFA + WS, and six replicates. Behavioral and thermographic assessments, body surface, foot and bed temperature, and locomotor health were performed on different days. No effect of bedding type on body temperature was observed; however, birds in the PFA group exhibited lower foot temperatures on Day 8. At 40 days, birds raised exclusively on PF or PFA exhibited longer sitting times, poorer locomotor scores, and a higher incidence of lesions. However, for broilers raised for up to 42 days, the exclusive use of PF resulted in locomotor impairment and aggravated joint lesions, suggesting the need for associations with WS to mitigate such effects.

本研究评估了在塑料地板(PF)上饲养的肉鸡的行为和运动障碍的发生率,并将添加和不添加纳米抗菌添加剂的肉鸡与使用木屑的传统系统进行了比较。选用1500只罗斯408系肉鸡,采用完全随机设计,分为木刨花(WS)、PF、PF + WS、添加塑料地板(PFA)和PFA + WS 5个处理,共6个重复。在不同的日子进行行为和热成像评估、体表、足部和床的温度以及运动健康。床褥类型对体温无影响;然而,PFA组的鸟类在第8天表现出较低的足部温度。在第40天,仅用PF或PFA饲养的鸟表现出更长的静坐时间,更差的运动评分和更高的病变发生率。然而,对于饲养42天的肉鸡,单独使用PF会导致运动障碍和关节病变加重,这表明需要与WS相关联来减轻这种影响。
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引用次数: 0
Morphological Study on the Coat Hair in Golden Takin 金羚牛被毛的形态研究。
IF 1.2 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2025-11-30 DOI: 10.1111/asj.70136
Yutaka Kawahara, Michiko Okada, Naoya Kawahara

The structure of coat hairs in golden takin (Budorcas taxicolor bedfordi) classified into the Bovidae family, living in harsher environmental conditions, is compared with the cattle's coat hair data. Morphological study and structural analysis with WAXD (wideangle X-ray diffraction) measurements were made on the hair samples, and the structural features were investigated. The overcoat hair in takin contained a continuous thick medullated tissue in its center while the undercoat hair was crimped finely and has no medullated tissue. Those morphologies of coat hairs will be effective to keep a vital temperature in harsh winter. The observation of the longitudinal section of the overcoat hair revealed the continuous dendritic framework-structured medullated tissue with air spaces and/or, in some cases, filling the spaces with wax-like granules. In the WAXD measurements of the summer coat hairs, similar α-keratin WAXD peaks were recorded except for the weak arc-shaped scattering peaks at around 2θ = 20.6°, 22.4°, which coincided with the peaks of the β′ crystal form of the fats.

对生活在较恶劣环境下的牛科金羚牛(Budorcas taxicolor bedfordi)的被毛结构与牛的被毛数据进行了比较。利用广角x射线衍射(WAXD)对毛发样品进行了形态学研究和结构分析,并对其结构特征进行了研究。被毛中部有连续的厚的有髓组织,而被毛卷曲细密,无髓组织。这些被毛的形态将有效地在严酷的冬天保持至关重要的温度。对被毛纵剖面的观察揭示了连续的树突框架结构的有髓组织,有空气空间和/或,在某些情况下,蜡状颗粒填充空间。在夏季被毛的WAXD测量中,除了在2θ = 20.6°,22.4°附近的弱弧形散射峰与脂肪的β′晶体形式的峰相吻合外,其他α-角蛋白的WAXD峰都是相似的。
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引用次数: 0
Plasma Free Amino Acid Profiles During Fattening Period Predict Carcass Traits in Female Japanese Black Cattle: A Preliminary Study 育肥期血浆游离氨基酸谱预测雌性日本黑牛胴体性状的初步研究。
IF 1.2 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2025-11-30 DOI: 10.1111/asj.70137
Hanwool Do, Toshiki Umeki, Takeo Miyake, Yasunari Yamamoto, Shozo Tomonaga

Early prediction of carcass traits, particularly carcass weight and beef marbling score, is critical for optimizing beef production efficiency. We explored prediction models for these carcass traits using plasma free amino acid and related metabolite profiles during the midfattening period (20 months) in female Japanese Black cattle (n = 52). Stepwise multiple regression analysis and leave-one-out cross-validation were used to construct and evaluate the model. However, analyses that included all cattle were unsatisfactory, likely due to data variability and/or insufficient sample size. When cattle were categorized into two fattening durations, conventional (n = 28) and longer term (n = 24), prediction models for both carcass traits were successfully developed and validated, suggesting that differences in the fattening duration influenced the quality of the prediction models. From a total of 24 amino acids and six related metabolites, five to nine amino acids were selected as key predictors, emphasizing the dominant role of amino acids in this study. These findings suggested that plasma free amino acid profiles are potential practical predictors of carcass traits in beef production. Future studies with larger, regionally diverse datasets and varying fattening durations are essential to enable broader industrial applications.

早期预测胴体性状,特别是胴体重和牛肉大理石纹评分,对优化牛肉生产效率至关重要。我们利用52头日本黑牛育肥中期(20个月)血浆游离氨基酸和相关代谢物谱,探索了这些胴体性状的预测模型。采用逐步多元回归分析和留一交叉验证对模型进行构建和评价。然而,包括所有牛的分析并不令人满意,可能是由于数据变异性和/或样本量不足。将牛分为常规(n = 28)和较长(n = 24)两种育肥期,成功建立并验证了两种胴体性状的预测模型,表明育肥期的差异影响了预测模型的质量。从24种氨基酸和6种相关代谢物中,筛选出5 ~ 9种氨基酸作为关键预测因子,强调了氨基酸在本研究中的主导作用。这些发现表明,血浆游离氨基酸谱是牛肉生产中胴体性状的潜在实用预测指标。未来研究更大的、不同地区的数据集和不同的增肥持续时间对于实现更广泛的工业应用至关重要。
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引用次数: 0
Comprehensive Phylogeographic Analysis Using mtDNA, SRY, and SNPs Markers Revealed Genetic Influence on Kyrgyzstan Goats via the Eurasian Steppe and the Oasis Routes 利用mtDNA、SRY和snp综合系统地理分析揭示欧亚草原和绿洲路线对吉尔吉斯斯坦山羊的遗传影响。
IF 1.2 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2025-11-30 DOI: 10.1111/asj.70135
Ryo Masuko, Yuka Katayama, Mari Inoue, Maho Masaoka, Fuki Kawaguchi, Shinji Sasazaki, Sanjar Sultankulov, Asankadyr Zhunushov, Takahiro Yonezawa, Masahide Nishibori, Hideyuki Mannen

Kyrgyzstan lies at the merge point of two ancient trade routes (the Eurasian Steppe and the Oasis Routes) and represents a key geographic region associated with probable goat propagation. Our study aimed to clarify goat propagation and the relationship between human activity and goats in Asia by investigating the genetic structure of Kyrgyzstan goats. We analyzed the mtDNA hypervariable region 1, the SRY 3′UTR, and genome-wide SNPs of 30 Kyrgyzstan goats. The mtDNA sequences were classified into three haplogroups: A (n = 25/30), D (n = 3/30), and G (n = 2/30), similar to that observed in Central Asia. Haplogroup G, mainly reported in the Middle East but absent in Central Asia, suggested a propagation route into Kyrgyzstan along the Oasis Route. For SRY haplotypes, Y1AB (n = 3/18) and Y2A (n = 15/18) were identified. The haplotype Y2A, largely distributed in Central Asia, implied genetic influence on Kyrgyzstan goats from the Eurasian Steppe Route. Genome-wide SNPs revealed that the Kyrgyzstan population formed a geographic cline extending from Russia to East Asia. Our findings reflect combined influences of the Eurasian Steppe and Oasis Routes on the genetic structure of Kyrgyzstan goats and illuminate their migration process through these routes.

吉尔吉斯斯坦位于两条古代贸易路线(欧亚草原和绿洲路线)的交汇处,是山羊可能繁殖的关键地理区域。本研究旨在通过对吉尔吉斯斯坦山羊遗传结构的研究,阐明亚洲山羊的繁殖和人类活动与山羊的关系。我们分析了30只吉尔吉斯斯坦山羊的mtDNA高变区1、SRY 3'UTR和全基因组snp。mtDNA序列可分为A (n = 25/30)、D (n = 3/30)和G (n = 2/30) 3个单倍群,与中亚地区相似。单倍群G主要在中东地区报道,在中亚地区未见报道,表明其沿绿洲路线进入吉尔吉斯斯坦。对于SRY单倍型,鉴定到Y1AB (n = 3/18)和Y2A (n = 15/18)。Y2A单倍型主要分布在中亚地区,暗示了来自欧亚草原路线的吉尔吉斯斯坦山羊的遗传影响。全基因组snp显示,吉尔吉斯斯坦人群形成了一个从俄罗斯延伸到东亚的地理渐变。我们的发现反映了欧亚草原和绿洲路线对吉尔吉斯斯坦山羊遗传结构的综合影响,并阐明了它们通过这些路线的迁移过程。
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引用次数: 0
The Effects of Using Spent Coffee Grounds as a Protein Source in the Concentrated Diet of Goats 用废咖啡渣作为蛋白质源在山羊浓缩日粮中的效果。
IF 1.2 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2025-11-25 DOI: 10.1111/asj.70134
Kanokwan Chukaew, Chotiwat Srisuwan, Pacharapon Inaoy, Somkiert Prasanpanich, K. Teepalak Rangubhet, Phongthorn Kongmun

This study evaluated the effects of substituting palm kernel cake (PKC) with spent coffee grounds (SCG) in goat concentrate diets on growth performance, feed utilization, and rumen fermentation. Sixteen male crossbred goats (15.7 ± 1.02 kg body weight) were randomly allocated to four dietary treatments in a completely randomized design: 0%, 25%, 50%, and 75% replacement of PKC with SCG. The substitution significantly influenced body weight gain (BWG), average daily gain (ADG), and feed intake (p < 0.05). Goats fed 0% and 25% SCG showed the highest BWG and ADG, whereas the 75% group exhibited the lowest (p < 0.05). Apparent digestibility of organic matter, crude protein, and acid detergent fiber peaked at 25% and 50% replacement levels (p < 0.05). Total volatile fatty acid concentrations declined with increasing SCG inclusion, showing a cubic response (p < 0.05). These findings indicate that incorporating SCG at up to 50% of the concentrate can enhance digestibility and fermentation without compromising growth. Nonetheless, a 25% replacement level yielded the most favorable economic performance. This study supports the potential of SCG as a cost-effective alternative feed ingredient to promote sustainable goat production.

本研究评价了用废咖啡渣(SCG)代替棕榈仁饼(PKC)对山羊精料日粮生长性能、饲料利用率和瘤胃发酵的影响。选用16只体重为15.7±1.02 kg的公杂交山羊,采用完全随机设计,随机分为4组饲粮处理:用SCG替代0%、25%、50%和75%的PKC。替代显著影响了猪体增重(BWG)、平均日增重(ADG)和采食量(p
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引用次数: 0
Adaptation Strategies to Climate Change: High Andean Plant Species as Ruminant Feed Alternatives 气候变化适应策略:安第斯高原植物物种作为反刍动物饲料替代品。
IF 1.2 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2025-11-16 DOI: 10.1111/asj.70131
Cristhian Camilo Otero Ayala, Laura Yiceth Sanabria Medina, Erika Mayerly Celis Celis, Mónica Andrea Botero Londoño, Julián Mauricio Botero Londoño

The understanding and use of plant resources, coupled with the strategic selection of forage species, play crucial roles in biodiversity preservation, enhanced biomass production, nutrient supply, and a reduced ecological footprint. This study aimed to characterize the forage potential of perennial plant species in high Andean ecosystems. Species naturally occurring in livestock production systems were identified and classified based on their botanical and agronomic characteristics. We assessed biomass production, bromatological composition, carbon sequestration, in vitro dry matter digestibility, and methane emissions. Among the 28 identified species, 17 presented optimal characteristics: high biomass, protein (> 7%), gross energy (> 3500 kcal kg−1), neutral detergent fiber (NDF), acid detergent fiber (ADF) percentages (< 42% and < 37%, respectively), and in vitro digestibility (> 40%). Plants with relatively high secondary metabolite contents exhibited reduced methane emissions. Carbon uptake varied from 3.27 to 35.41 g C m−2 or plant. The recognized forage potential of these perennial plant species offers a sustainable option for integration into ruminant feeding systems and silvopastoral practices in the tropical Andes. This strategic integration presents a viable approach for mitigating the impacts of livestock farming in response to climate change.

对植物资源的认识和利用,加上饲料品种的战略性选择,在保护生物多样性、提高生物量生产、养分供应和减少生态足迹方面发挥着至关重要的作用。本研究旨在探讨安第斯高原生态系统中多年生植物的牧草潜力。根据其植物学和农艺特征对牲畜生产系统中自然存在的物种进行了鉴定和分类。我们评估了生物质产量、色相组成、碳固存、体外干物质消化率和甲烷排放。结果表明,其中17种生物量高,蛋白质含量(7%),总能量(3500 kcal kg-1),中性洗涤纤维(NDF)和酸性洗涤纤维(ADF)含量(40%)。次生代谢物含量较高的植株甲烷排放减少。植物的碳吸收量在3.27 ~ 35.41 g C m-2之间。这些多年生植物物种公认的饲料潜力为热带安第斯山脉的反刍动物喂养系统和森林放牧提供了可持续的选择。这种战略整合为减轻畜牧业对气候变化的影响提供了一种可行的方法。
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引用次数: 0
Relationship Between Methane Emissions and Intestinal Methanogenic Microbiota in Micro-Mini Pigs 微型猪肠道产甲烷菌群与甲烷排放的关系
IF 1.2 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2025-11-14 DOI: 10.1111/asj.70130
Maki Hirata, Eiko Nakashima, Iori Suenaga, Fumiki Morimatsu

Methane production in pigs has been less frequently studied in comparison to cattle. In this study, we developed an original greenhouse gas (GHG) monitoring system designed for pigs and explored the relationship between GHG emissions measured using this system and methane-producing archaea in the porcine gut. The system comprises a semi-closed monitoring chamber and a photoacoustic gas monitor capable of real-time gas concentration monitoring. A gut microbiota analysis was conducted in parallel with the GHG measurements. Microbiota analysis revealed that the genus Methanobrevibacter dominated the intestinal microbiota of micro-mini pigs, followed by the family Methanomethylophilaceae and genus Methanosphaera. Analysis of GHG emissions indicated that carbon dioxide emissions were correlated with body weight, while methane production was not associated with body weight, but rather with the abundance of the genus Methanobrevibacter in the gut. Methane production in the lower gastrointestinal tract of pigs was thought to be positively correlated with dietary fiber intake, and the composition of the intestinal microbiota may also play a role in methane generation. These findings will contribute to advancing research on GHG emission reductions in livestock production. However, a more comprehensive understanding of archaeal diversity requires further detailed analyses using methods targeted specifically at archaea.

与牛相比,对猪体内甲烷产量的研究较少。在这项研究中,我们开发了一种为猪设计的原始温室气体(GHG)监测系统,并探讨了使用该系统测量的温室气体排放量与猪肠道中产生甲烷的古细菌之间的关系。该系统包括一个半封闭的监测室和一个能够实时监测气体浓度的光声气体监测器。在进行温室气体测量的同时进行了肠道微生物群分析。微生物区系分析显示,微型猪肠道微生物区系以methanobrebacter属为主,其次为methanomethylolophilaceae科和Methanosphaera属。温室气体排放分析表明,二氧化碳排放量与体重相关,而甲烷产量与体重无关,而是与肠道中甲烷预防菌属的丰度相关。猪下胃肠道的甲烷产量被认为与膳食纤维摄入量呈正相关,肠道微生物群的组成也可能在甲烷的产生中发挥作用。这些发现将有助于推进畜牧业生产中温室气体减排的研究。然而,要更全面地了解古细菌的多样性,需要使用专门针对古细菌的方法进行进一步的详细分析。
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引用次数: 0
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