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Is lifestyle different in male partners experiencing recurrent pregnancy loss compared to men fathering a live birth? 与生育过活产婴儿的男性相比,反复妊娠失败的男性伴侣的生活方式是否有所不同?
IF 3.2 2区 医学 Q1 ANDROLOGY Pub Date : 2024-10-07 DOI: 10.1111/andr.13764
Johanna Lindman, Kilian Vomstein, Pia Egerup, Maria Christine Krog, Henriette Svarre Nielsen

Background

Recurrent pregnancy loss is characterized by three or more consecutive pregnancy losses. Although the causes of recurrent pregnancy loss are often unknown, chromosomal defects and fetal anomalies account for a significant proportion of cases. Previous research has primarily focused on maternal factors, but recent attention has shifted to the role of male lifestyle factors.

Objectives

This study examined how male lifestyle factors and chronic illnesses affect recurrent pregnancy loss in a Danish cohort. Objectives included analyzing demographic and clinical features, as well as assessing lifestyle factors and pregnancy outcomes.

Materials and methods

We included 741 males referred to the Danish recurrent pregnancy loss unit between 2009 and 2021, alongside a control group of 1173 males from the PREGCO study. Data on demography, clinical features, lifestyle factors, and pregnancy outcomes were collected and analyzed.

Results

The recurrent pregnancy loss group had a higher mean age compared to the controls. Although there was a trend suggesting a higher prevalence of obesity in the recurrent pregnancy loss group, statistical significance was not reached. The prevalence of chronic illnesses was similar in both groups. In the recurrent pregnancy loss group, a higher body mass index and history of previous or current smoking were associated with a lower pregnancy rate, and men who never smoked had an increased likelihood of achieving pregnancy. However, these associations lost significance after adjusting for potential confounders.

Discussion

The study suggests an association between male obesity and smoking, and decreased pregnancy rates after referral for recurrent pregnancy loss. However, further research is needed to understand the underlying mechanisms and establish causality in this association.

Conclusion

The study reveals potential associations between male smoking, male obesity, and reduced pregnancy rates in individuals referred for recurrent pregnancy loss. These findings emphasize the importance of considering male lifestyle factors in the evaluation and management of recurrent pregnancy loss.

背景:复发性妊娠是指连续三次或三次以上的妊娠失败。虽然导致复发性妊娠失败的原因往往不明,但染色体缺陷和胎儿畸形占很大比例。以往的研究主要集中在母体因素上,但最近的注意力已转移到男性生活方式因素的作用上:本研究调查了丹麦队列中男性生活方式因素和慢性疾病对复发性妊娠失败的影响。目标包括分析人口统计学和临床特征,以及评估生活方式因素和妊娠结局:我们纳入了 2009 年至 2021 年期间转诊至丹麦复发性妊娠丢失科的 741 名男性,以及来自 PREGCO 研究的 1173 名男性对照组。我们收集并分析了有关人口统计学、临床特征、生活方式因素和妊娠结局的数据:结果:与对照组相比,复发性妊娠失败组的平均年龄更高。虽然有趋势表明复发性妊娠失败组的肥胖率较高,但未达到统计学意义。两组的慢性病患病率相似。在复发性妊娠失败组中,体重指数较高、以前或现在有吸烟史的男性怀孕率较低,而从不吸烟的男性怀孕的可能性较高。然而,在对潜在的混杂因素进行调整后,这些关联失去了显著性:讨论:该研究表明,男性肥胖和吸烟与反复妊娠失败转诊后怀孕率降低之间存在关联。结论:该研究揭示了男性肥胖与吸烟之间的潜在联系:该研究揭示了男性吸烟、男性肥胖与因复发性妊娠丢失而转诊的患者妊娠率降低之间的潜在关联。这些发现强调了在评估和处理复发性妊娠失败时考虑男性生活方式因素的重要性。
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引用次数: 0
Exploring the impact of sexual positions on ejaculation: Insights from a survey study by the Andrology Working Group of the Society of Urological Surgery in Turkey. 探索性姿势对射精的影响:土耳其泌尿外科学会男性学工作组调查研究的启示。
IF 3.2 2区 医学 Q1 ANDROLOGY Pub Date : 2024-10-04 DOI: 10.1111/andr.13775
Murat Gül, Ali Şahin, Çağrı Doğan, Gökhan Çeker, Emre Altıntaş, Hasan Deliktaş, Murat Demir, Aldülmecit Yavuz, Adem Altunkol, Doğan Değer, Mehmet Kaynar, Berkan Duran, Tuncay Toprak, Tuncer Bahçeci, Ümit Gül

Background: Sexual position is one of the several aspects that affects ejaculation control, which is essential for sexual pleasure. Still little is known, nevertheless, about the connection between sexual positions and ejaculation duration.

Objective: To investigate the impact of various sexual positions on the duration of ejaculation and gain a deeper understanding of the elements that influence the ability to control ejaculation.

Method: An online survey was carried out on a sample of 1904 heterosexual men between the ages of 18-65 years. Premature ejaculation (PE) diagnostic tool was used to define PE. Demographic, behavioral, and physiological traits that are linked to PE and non-PE groups were collected. The participants listed their preferred sexual positions and the ones they changed to during ejaculating. Also, analyses were performed between male/female active or deep/shallow thrust positions and PE status.

Results: Although there were no appreciable variations in age or circumcision between PE and non-PE groups, the age of first sexual experience was associated with PE status. Groups also varied in the number of weekly ejaculations, the duration of the favored ejaculations, and the characteristics of the erection. The most preferred sexual posture was the doggy style regardless of the group. When the non-PE group felt to ejaculate, they preferred to change the position significantly more than the PE group (74% vs 67.2%; p < 0.05). However, when participants felt ejaculate, non-PE participants tended to switch to shallow thrusting positions significantly more than PE participants, who preferred deeper positions (27.1% vs. 18%; p < 0.05).

Conclusion: This study underlines the relevance of considering sexual positions in controlling PE. Modifying positions during sexual intercourse may offer a non-pharmacological therapeutic alternative for improving ejaculation control. Future studies in this field might help to create tailored PE treatment strategies.

背景:性爱姿势是影响射精控制的几个方面之一,而射精控制对性快感至关重要。然而,人们对性爱姿势与射精持续时间之间的关系仍然知之甚少:调查各种性爱姿势对射精持续时间的影响,深入了解影响控制射精能力的因素:对 1904 名年龄在 18-65 岁之间的异性恋男性样本进行了在线调查。早泄(PE)诊断工具用于定义早泄。调查还收集了与 PE 和非 PE 群体相关的人口、行为和生理特征。参与者列出了他们喜欢的性爱体位以及在射精时改变的体位。此外,还对男性/女性主动或深浅插入体位与 PE 状态进行了分析:结果:虽然 PE 组和非 PE 组在年龄或包皮环切术方面没有明显差异,但首次性经历的年龄与 PE 状态有关。各组在每周射精次数、喜欢的射精持续时间和勃起特征方面也存在差异。无论在哪个组别,最喜欢的性爱姿势都是狗爬式。当非 PE 组感到要射精时,他们喜欢改变姿势的比例明显高于 PE 组(74% 对 67.2%;P 结论:这一研究强调了在性生活中考虑射精姿势的重要性:本研究强调了在控制 PE 时考虑性交体位的重要性。在性交过程中改变体位可能是改善射精控制的一种非药物治疗方法。未来在这一领域的研究可能有助于制定量身定制的 PE 治疗策略。
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引用次数: 0
hACE2 upregulation and participation of macrophages and clear cells in the immune response of epididymis to SARS-CoV-2 in K18-hACE2 mice. K18-hACE2 小鼠附睾对 SARS-CoV-2 的免疫反应中 hACE2 的上调以及巨噬细胞和透明细胞的参与。
IF 3.2 2区 医学 Q1 ANDROLOGY Pub Date : 2024-10-03 DOI: 10.1111/andr.13755
André Acácio Souza da Silva, Salmo Azambuja de Oliveira, Maria Agustina Battistone, Barry Thomas Hinton, Paulo Sérgio Cerri, Estela Sasso-Cerri

Background: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus caused the coronavirus disease 2019 pandemic, and the prevalence of deaths among men is higher than among women. The epididymis, divided into caput, corpus, and cauda, shows a region-specific immunity. The K18-hACE2 mouse expresses human angiotensin-converting enzyme 2 (hACE2), the receptor that allows SARS-CoV-2 infection. However, studies using this transgenic mouse to evaluate the impact of this viral infection in epididymis have not yet been performed.

Objectives: We evaluated the expression of hACE2 in the epididymis of SARS-CoV-2-infected K18-hACE2 mice, and assessed the epididymal immune response, focusing on F4/80+ mononuclear phagocytes and tumor necrosis factor-alpha expression.

Materials and methods: The following analyses were performed in the epididymal sections of infected mice: epithelial height and duct diameter, birefringent collagen, Terminal deoxynucleotidyl Transferase-mediated dUTP Nick End Labelling, immunoreactions for detection of hACE2, spike, FGF, V-ATPase, F4/80, tumor necrosis factor-alpha, and iNOS. Viral particles were identified under electron microscopy. hACE2, Rigi, Tgfb1 and Tnfa expression were also evaluated by real-time quantitative polymerase chain reaction.

Results: All epididymal regions expressed hACE2, which increased in all epididymal regions in the infected mice. However, the caput appeared to be the most infected region. Despite this, the caput region showed minimal changes while the cauda showed significant epithelial changes associated with increased iNOS immunoexpression. The F4/80+ mononuclear phagocyte area increased significantly in both stroma and epithelium. In addition to the epithelial and stromal mononuclear phagocytes, tumor necrosis factor-alpha was also detected in clear cells, whose cytoplasm showed a significant increase of this cytokine in the infected animals.

Discussion and conclusion: The K18-hACE2 mouse is a useful model for evaluating the impact of SARS-CoV-2 infection in the epididymis. The infection induced hACE2 upregulation, favoring the virulence in the epididymis. The epididymal regions responded differentially to infection, and the activation of F4/80+ mononuclear phagocytes associated with the increased tumor necrosis factor-alpha immunolabeling in clear cells indicates a role of clear cells/mononuclear phagocytes immunoregulatory mechanisms in the epididymal immune response to SARS-CoV-2 infection.

背景:严重急性呼吸系统综合征冠状病毒 2(SARS-CoV-2)病毒导致了冠状病毒病 2019 年的大流行,男性的死亡发病率高于女性。附睾分为冠状沟、冠状沟和尾状沟,表现出区域特异性免疫。K18-hACE2 小鼠表达人类血管紧张素转换酶 2(hACE2),这种受体可使 SARS-CoV-2 感染。然而,利用这种转基因小鼠评估这种病毒感染对附睾的影响的研究尚未开展:我们评估了 hACE2 在感染 SARS-CoV-2 的 K18-hACE2 小鼠附睾中的表达情况,并评估了附睾免疫反应,重点是 F4/80+ 单核吞噬细胞和肿瘤坏死因子-α的表达情况:对感染小鼠的附睾切片进行了以下分析:上皮高度和导管直径、双折射胶原、末端脱氧核苷酸转移酶介导的dUTP尼克末端标记、检测hACE2、穗状病毒、成纤维细胞生长因子、V-ATP酶、F4/80、肿瘤坏死因子-α和iNOS的免疫反应。还通过实时定量聚合酶链反应评估了 hACE2、Rigi、Tgfb1 和 Tnfa 的表达:结果:所有附睾区域都表达了hACE2,受感染小鼠所有附睾区域的hACE2都有所增加。然而,绒毛区似乎是受感染最严重的区域。尽管如此,绒毛区的变化很小,而尾部则出现了明显的上皮变化,并伴有 iNOS 免疫表达的增加。基质和上皮中的 F4/80+ 单核吞噬细胞面积均显著增加。除了上皮细胞和基质单核吞噬细胞外,还在透明细胞中检测到肿瘤坏死因子-α,感染动物的透明细胞的细胞质中该细胞因子显著增加:K18-hACE2小鼠是评估SARS-CoV-2感染对附睾影响的有用模型。感染诱导 hACE2 上调,有利于附睾的毒力。附睾区域对感染的反应不同,F4/80+单核吞噬细胞的激活与透明细胞中肿瘤坏死因子-α免疫标记的增加有关,这表明透明细胞/单核吞噬细胞的免疫调节机制在附睾对SARS-CoV-2感染的免疫反应中发挥作用。
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引用次数: 0
PWWP3A deficiency accelerates testicular senescence in aged mice. PWWP3A 缺乏会加速老龄小鼠睾丸的衰老。
IF 3.2 2区 医学 Q1 ANDROLOGY Pub Date : 2024-10-03 DOI: 10.1111/andr.13774
Zhen Chen, Cong Liu, Wei Qu, Yan Han, Xiaoyu Zhu, Zejia Li, Dupeng Ma, Mengya Huang, Weihao Gong, Qi Sun, Junhao Lei, Rui Guo, Mengcheng Luo

Background: The PWWP domain-containing proteins are involved in chromatin-associated biological processes, including transcriptional regulation and DNA repair, and most of them are significant for gametogenesis and early embryonic development in mammals. PWWP3A, one of the PWWP domain proteins, is a reader of H3K36me2/H3K36me3 and a response factor to DNA damage. However, the physiological role of PWWP3A in spermatogenesis and fertility remains unclear.

Objective: The goal of this study was to explore the function and mechanism of PWWP3A in the process of spermatogenesis.

Materials and methods: We generated V5-Pwwp3a KI mice and PWWP3A polyclonal antibody to observe the localization of PWWP3A in vivo. Meanwhile, Pwwp3a KO mice was used to explore the function in spermatogenesis.

Results: We reported that PWWP3A is a predominant expression in the testis of mice. During spermatogenesis, PWWP3A exhibits the temporal expression from early-pachytene to the round spermatids. The results of spermatocyte spreading and immunostaining showed that PWWP3A aggregated on the XY body, which then diffused as the XY chromosome separated at late-diplotene. Although the depletion of PWWP3A had no obvious reproductive defects in young male mice, there were observed morphological abnormalities in sperm heads. Immunoprecipitation demonstrated the interaction of PWWP3A with DNA repair proteins SMC5/6; however, PWWP3A deficiency did not result in any meiotic defects. Notably, the testes of aged male Pwwp3a KO mice displayed pronounced degeneration, and were characterized by the presence of vacuolated seminiferous tubules. Furthermore, RNA-seq analysis revealed an upregulation in the expression of genes which may be involving in immunoregulatory and inflammatory response pathways in aged Pwwp3a KO mice with testicular degeneration.

Conclusions: Our study showed that PWWP3A was highly enriched in the mouse testis, and the Pwwp3a KO mice were fertile. However, the aged Pwwp3a KO male mice displayed testicular atrophy that may be due to changes in the immune micro-environment or abnormal repair of DNA damage.

背景:含PWWP结构域的蛋白参与染色质相关的生物学过程,包括转录调控和DNA修复,其中大多数对哺乳动物的配子发生和早期胚胎发育具有重要意义。PWWP3A 是 PWWP 结构域蛋白之一,是 H3K36me2/H3K36me3 的阅读器,也是 DNA 损伤的反应因子。然而,PWWP3A 在精子发生和生育中的生理作用仍不清楚:本研究旨在探索 PWWP3A 在精子发生过程中的功能和机制:材料:我们制备了V5-Pwwp3a KI小鼠和PWWP3A多克隆抗体,以观察PWWP3A在体内的定位。同时,利用Pwwp3a KO小鼠探讨其在精子发生中的功能:结果:我们发现PWWP3A在小鼠睾丸中呈优势表达。结果:我们发现PWWP3A在小鼠睾丸中呈优势表达,在精子发生过程中,PWWP3A表现出从早幼粒细胞到圆形精子的时间性表达。精母细胞扩散和免疫染色的结果显示,PWWP3A聚集在XY体上,然后随着XY染色体在二分裂后期的分离而扩散。虽然消耗 PWWP3A 对幼年雄性小鼠没有明显的生殖缺陷,但却观察到精子头部的形态异常。免疫沉淀显示 PWWP3A 与 DNA 修复蛋白 SMC5/6 相互作用;然而,PWWP3A 的缺乏并没有导致任何减数分裂缺陷。值得注意的是,Pwwp3a KO 雄性小鼠的睾丸出现了明显的退化,其特征是存在空泡化的曲细精管。此外,RNA-seq分析显示,在睾丸退化的Pwwp3a KO小鼠中,可能参与免疫调节和炎症反应通路的基因表达上调:我们的研究表明,PWWP3A 在小鼠睾丸中高度富集,Pwwp3a KO 小鼠具有生育能力。结论:我们的研究表明,PWWP3A在小鼠睾丸中高度富集,且Pwwp3a KO小鼠具有生育能力,但老年Pwwp3a KO雄性小鼠出现睾丸萎缩,这可能是由于免疫微环境发生变化或DNA损伤修复异常所致。
{"title":"PWWP3A deficiency accelerates testicular senescence in aged mice.","authors":"Zhen Chen, Cong Liu, Wei Qu, Yan Han, Xiaoyu Zhu, Zejia Li, Dupeng Ma, Mengya Huang, Weihao Gong, Qi Sun, Junhao Lei, Rui Guo, Mengcheng Luo","doi":"10.1111/andr.13774","DOIUrl":"https://doi.org/10.1111/andr.13774","url":null,"abstract":"<p><strong>Background: </strong>The PWWP domain-containing proteins are involved in chromatin-associated biological processes, including transcriptional regulation and DNA repair, and most of them are significant for gametogenesis and early embryonic development in mammals. PWWP3A, one of the PWWP domain proteins, is a reader of H3K36me2/H3K36me3 and a response factor to DNA damage. However, the physiological role of PWWP3A in spermatogenesis and fertility remains unclear.</p><p><strong>Objective: </strong>The goal of this study was to explore the function and mechanism of PWWP3A in the process of spermatogenesis.</p><p><strong>Materials and methods: </strong>We generated V5-Pwwp3a KI mice and PWWP3A polyclonal antibody to observe the localization of PWWP3A in vivo. Meanwhile, Pwwp3a KO mice was used to explore the function in spermatogenesis.</p><p><strong>Results: </strong>We reported that PWWP3A is a predominant expression in the testis of mice. During spermatogenesis, PWWP3A exhibits the temporal expression from early-pachytene to the round spermatids. The results of spermatocyte spreading and immunostaining showed that PWWP3A aggregated on the XY body, which then diffused as the XY chromosome separated at late-diplotene. Although the depletion of PWWP3A had no obvious reproductive defects in young male mice, there were observed morphological abnormalities in sperm heads. Immunoprecipitation demonstrated the interaction of PWWP3A with DNA repair proteins SMC5/6; however, PWWP3A deficiency did not result in any meiotic defects. Notably, the testes of aged male Pwwp3a KO mice displayed pronounced degeneration, and were characterized by the presence of vacuolated seminiferous tubules. Furthermore, RNA-seq analysis revealed an upregulation in the expression of genes which may be involving in immunoregulatory and inflammatory response pathways in aged Pwwp3a KO mice with testicular degeneration.</p><p><strong>Conclusions: </strong>Our study showed that PWWP3A was highly enriched in the mouse testis, and the Pwwp3a KO mice were fertile. However, the aged Pwwp3a KO male mice displayed testicular atrophy that may be due to changes in the immune micro-environment or abnormal repair of DNA damage.</p>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142370792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Machine learning-based classification of varicocoele grading: A promising approach for diagnosis and treatment optimization. 基于机器学习的静脉曲张分级分类:一种有望优化诊断和治疗的方法。
IF 3.2 2区 医学 Q1 ANDROLOGY Pub Date : 2024-10-03 DOI: 10.1111/andr.13776
Mehmet Vehbi Kayra, Ali Şahin, Serdar Toksöz, Mehmet Serindere, Emre Altıntaş, Halil Özer, Murat Gül

Background: Varicocoele is a correctable cause of male infertility. Although physical examination is still being used in diagnosis and grading, it gives conflicting results when compared to ultrasonography-based varicocoele grading.

Objectives: We aimed to develop a multi-class machine learning model for the grading of varicocoeles based on ultrasonographic measurements.

Method: Between January and May 2024, we enrolled unilateral varicocoele patients at an infertility clinic, assessing their varicocoele stages using the Dubin and Amelar system. We measured vascular diameter and reflux time at the testicular apex and the subinguinal region ultrasonography in both the supine and standing positions. Using these measurements, we developed four multi-class machine learning models, evaluating their performance metrics and determining which patient position and projection were most influential in varicocoele grading.

Results: We included 248 patients with unilateral varicocoele in the study, their average age was 26.61 ± 4.95 years old. Of these, 212 had left-sided and 36 had right-sided varicocoeles. According to the Dubin and Amelar system, there were 66 grade I, 96 grade II, and 86 grade III varicocoeles. Among the models we created, the random forest (RF) model performed best, with an overall accuracy of 0.81 ± 0.06, an F1 score of 0.79 ± 0.02, a sensitivity of 0.69 ± 0.02, and a specificity of 0.8 ± 0.03. Vascular diameter measurement at the testicular apex in the supine position had the most impact on grading across all models. In support vector machine and multi-layer perceptron models, reflux time measurements from the subinguinal projection in the standing position contributed the most, while in RF and k-nearest neighbors models, measurements from the subinguinal projection in the supine position were the most influential.

Conclusions: Machine learning methods have demonstrated superior accuracy in predicting disease compared to traditional statistical regressions and nomograms. These advancements hold promise for clinically automated prediction of varicocoele grades in patients. Tailored varicocoele grading for individuals has the potential to enhance treatment effectiveness and overall quality of life.

背景介绍精索静脉曲张是导致男性不育的一个可纠正的原因。尽管体格检查仍被用于诊断和分级,但与基于超声波的精索静脉曲张分级相比,体格检查得出的结果相互矛盾:我们旨在开发一种基于超声波测量的精索静脉曲张分级的多类机器学习模型:2024年1月至5月期间,我们在一家不孕不育诊所招募了单侧精索静脉曲张患者,使用Dubin和Amelar系统评估了他们的精索静脉曲张分期。我们测量了仰卧位和站立位睾丸顶点和腹股沟下区超声波的血管直径和回流时间。利用这些测量结果,我们建立了四个多类机器学习模型,评估了它们的性能指标,并确定了哪种患者体位和投影对精索静脉曲张分级最有影响:我们将 248 名单侧静脉曲张患者纳入研究,他们的平均年龄为 26.61 ± 4.95 岁。其中,212 例为左侧静脉曲张,36 例为右侧静脉曲张。根据 Dubin 和 Amelar 系统,静脉曲张分为 I 级 66 例、II 级 96 例和 III 级 86 例。在我们创建的模型中,随机森林(RF)模型表现最佳,总体准确率为 0.81 ± 0.06,F1 得分为 0.79 ± 0.02,灵敏度为 0.69 ± 0.02,特异性为 0.8 ± 0.03。在所有模型中,仰卧位睾丸顶点的血管直径测量对分级的影响最大。在支持向量机和多层感知器模型中,站立位时腹股沟下投影的回流时间测量结果影响最大,而在RF和k近邻模型中,仰卧位时腹股沟下投影的测量结果影响最大:与传统的统计回归和提名图相比,机器学习方法在预测疾病方面表现出更高的准确性。这些进步为临床上自动预测患者的静脉曲张等级带来了希望。为个人量身定制的静脉曲张分级有可能提高治疗效果和整体生活质量。
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引用次数: 0
Effect of antidepressants on ejaculation dysfunction in patients with depression and anxiety: A systematic review and network meta-analysis. 抗抑郁药对抑郁症和焦虑症患者射精功能障碍的影响:系统综述和网络荟萃分析。
IF 3.2 2区 医学 Q1 ANDROLOGY Pub Date : 2024-09-30 DOI: 10.1111/andr.13770
Qihua Wang, Zhunan Xu, Xiangyu Chen, Li Liu, Xiaoqiang Liu

Introduction: Antidepressants may lead to a series of sexual adverse effects (SAEs), among which ejaculation dysfunction (EjD) is often overlooked by clinicians. The purpose of the present network meta-analysis was to assist drug adjustment by comparing and ranking the incidence of EjD among various antidepressants.

Methods: Relevant studies were retrieved from PubMed, Embase, Scopus, Web of Science, ClinicalTrials.gov, and other additional records. Eligible randomized controlled trials (RCTs) assessed the rate of EjD in patients with major depressive disorder (MDD) and anxiety disorder after taking anti-depressants. The incidences of EjD, erectile dysfunction (ED), decreased libido (DL), adverse events (AE), withdrawal due to adverse events (WDAE) and withdrawal due to lack of efficacy (WDLE) were pooled using odds ratio (OR) with their 95% confidence intervals (CI). The values of surface under the cumulative ranking curve (SUCRA) helped to rank the risk of each outcome in different antidepressants.

Results: Thirty RCTs comprising 18,157 patients were included. Results of all node-splitting analysis demonstrated no statistical inconsistency (all P > 0.05). Clomipramine (OR 42.11, 95% CI [9.90, 179.08]), WS5570 (OR 28.99, 95% CI [1.48, 568.97]) and paroxetine (OR 18.63, 95% CI [9.33, 37.23]) had significant risk of EjD comparing to placebo. Additionally, duloxetine (OR 7.37, 95% CI [2.61, 20.78]), clomipramine (OR 5.29, 95% CI [1.72, 16.25]), paroxetine (OR 3.75, 95% CI [1.37, 10.26]) and escitalopram (OR 3.04, 95% CI [1.20, 7.71]) presented higher risk of ED comparing to placebo. Agomelatine, levomilnacipran, vortioxetine, trazodone, vilazodone, fluvoxamine and imipramine exhibited similar incidence of EjD with placebo (all P > 0.05). Besides, trazodone, vilazodone and vortioxetine had the top-five SUCRA values in each of SAEs (EjD, ED and DL), and agomelatine might be alternative in EjD and DL. Considering about AE, WDAE and WDLE, vilazodone appeared to offer more satisfactory performance across all these aspects.

Conclusions: For patients undergoing SAEs following the administration of antidepressants, trazodone, vortioxetine, vilazodone and agomelatine are alternative antidepressants.

简介抗抑郁药可能导致一系列性不良反应(SAEs),其中射精功能障碍(Ejaculation dysfunction,EjD)常常被临床医生忽视。本网络荟萃分析的目的是通过比较各种抗抑郁药的射精障碍发生率并对其进行排序,从而帮助调整用药:方法:从 PubMed、Embase、Scopus、Web of Science、ClinicalTrials.gov 和其他附加记录中检索相关研究。符合条件的随机对照试验(RCT)评估了重度抑郁障碍(MDD)和焦虑症患者服用抗抑郁药后的EjD发生率。研究人员使用几率比(OR)及其 95% 置信区间(CI)对 EjD、勃起功能障碍(ED)、性欲减退(DL)、不良事件(AE)、因不良事件而停药(WDAE)和因缺乏疗效而停药(WDLE)的发生率进行了汇总。累积排序曲线下表面值(SUCRA)有助于对不同抗抑郁药的每种结果的风险进行排序:结果:共纳入了 30 项 RCT,包括 18,157 名患者。所有节点拆分分析的结果均未显示出统计学上的不一致性(P 均大于 0.05)。与安慰剂相比,氯米帕明(OR 42.11,95% CI [9.90,179.08])、WS5570(OR 28.99,95% CI [1.48,568.97])和帕罗西汀(OR 18.63,95% CI [9.33,37.23])具有显著的 EjD 风险。此外,与安慰剂相比,度洛西汀(OR 7.37,95% CI [2.61,20.78])、氯米帕明(OR 5.29,95% CI [1.72,16.25])、帕罗西汀(OR 3.75,95% CI [1.37,10.26])和艾司西酞普兰(OR 3.04,95% CI [1.20,7.71])的ED风险更高。阿戈美拉汀、左米那西普兰、伏替西汀、曲唑酮、维拉佐酮、氟伏沙明和丙咪嗪的EjD发生率与安慰剂相似(P均>0.05)。此外,曲唑酮、维拉唑酮和伏替西汀在每种 SAEs(EjD、ED 和 DL)中的 SUCRA 值均居前五位,而阿戈美拉汀可能是 EjD 和 DL 的替代药物。考虑到AE、WDAE和WDLE,维拉唑酮在所有这些方面的表现似乎更令人满意:结论:对于服用抗抑郁药后出现 SAE 的患者,曲唑酮、伏替西汀、维拉佐酮和阿戈美拉汀是替代抗抑郁药。
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引用次数: 0
Penile curvature severity in patients with Peyronie's disease does not correlate with dynamic color doppler duplex ultrasound parameters: findings from a real-life cross-sectional study. 佩罗尼氏病患者阴茎弯曲的严重程度与动态彩色多普勒双相超声参数无关:一项实际横断面研究的结果。
IF 3.2 2区 医学 Q1 ANDROLOGY Pub Date : 2024-09-30 DOI: 10.1111/andr.13771
Alessandro Bertini, Edoardo Pozzi, Federico Belladelli, Massimiliano Raffo, Christian Corsini, Fausto Negri, Giacomo Musso, Francesco Cattafi, Riccardo Ramadani, Luigi Candela, Alessia d' Arma, Francesco Montorsi, Andrea Salonia

Introduction: There is inconsistent data regarding the possible inaccuracies in dynamic penile color Doppler duplex ultrasound (CDDU) measurements in men with penile curvature because of Peyronie's disease (PD). We sought to explore the relationship between the degree of penile curvature and CDDU parameters in men with PD.

Materials and methods: Comprehensive data from 154 consecutive men presenting with PD as their primary complaint at a single academic center were prospectively collected and analyzed. All men underwent CDDU. Penile curvature was measured using a goniometer at time of maximum erection during CDDU. Patients were grouped based on CDDU parameters into-normal (average peak systolic velocity [PSV] ≥ 35 cm/s and resistance index [RI] ≥ 0.85) and pathological CDDU (average PSV < 35 cm/s and/or RI < 0.85). Descriptive statistics was used to compare the two subcohorts. Linear regression models were fitted to explore the association between the degree of penile curvature and dynamic CDDU parameters.

Results: Overall, the median interquartile range (IQR) age was 56 (48-63) years. The median (IQR) PSV and degree of penile curvature were 48.8 cm/s (37.9-58.5) and 40 degrees (30-60), respectively. At CDDU, the degrees of penile curvature were as follows: 10-30 degrees in 63 (40.9%) men, 30-70 degrees in 70 (45.5%) men, and 70-90 degrees in 21 (13.6%) men, respectively. Of all, 116 (75.3%) patients showed a PSV > 35 cm/s and RI ≥ 0.85. Patients with pathologic vs. normal CDDU parameters did not differ in median (IQR) curvature (32.5° [30°-58.7°] vs. 40° [30°-65°], p = 0.5) or in the distribution across curvature range groups. Linear regression analysis revealed that the degree of penile curvature did not significantly correlate with PSV at CDDU (coefficient: 0.06, p = 0.3).

Conclusions: Our study confirms the lack of a significant correlation between the severity of penile curvature and CDDU parameters in men presenting with PD. These findings emerge to be relevant in terms of a more accurate management work-up for PD patients and hold insightful medicolegal implications and in the real-life setting.

简介:关于因患佩罗尼氏病(PD)而导致阴茎弯曲的男性的动态阴茎彩色多普勒双工超声(CDDU)测量可能存在误差的数据并不一致。我们试图探索佩罗尼氏病男性患者阴茎弯曲程度与 CDDU 参数之间的关系:我们前瞻性地收集并分析了在一家学术中心连续就诊的154名以PD为主诉的男性的综合数据。所有男性均接受了 CDDU 检查。在 CDDU 过程中,使用测角器在阴茎最大勃起时测量阴茎弯曲度。根据 CDDU 参数将患者分为正常(平均收缩峰值速度 [PSV] ≥ 35 cm/s,阻力指数 [RI] ≥ 0.85)和病理 CDDU(平均 PSV 结果)两组:总体而言,中位四分位距(IQR)年龄为 56(48-63)岁。PSV 和阴茎弯曲度的中位数(IQR)分别为 48.8 厘米/秒(37.9-58.5)和 40 度(30-60)。在 CDDU,阴茎弯曲度如下:分别有 63 名(40.9%)男性的阴茎弯曲度为 10-30 度,70 名(45.5%)男性的阴茎弯曲度为 30-70 度,21 名(13.6%)男性的阴茎弯曲度为 70-90 度。在所有患者中,116 名(75.3%)患者的 PSV > 35 cm/s,RI ≥ 0.85。病理与正常 CDDU 参数的患者在中位(IQR)曲率(32.5° [30°-58.7°] 与 40° [30°-65°],P = 0.5)或曲率范围各组的分布上没有差异。线性回归分析表明,阴茎弯曲度与 CDDU 时的 PSV 没有显著相关性(系数:0.06,P = 0.3):我们的研究证实,阴茎弯曲的严重程度与 CDDU 参数之间没有明显的相关性。这些发现有助于对阴茎短小症患者进行更准确的管理,在现实生活中也具有深刻的医学法律意义。
{"title":"Penile curvature severity in patients with Peyronie's disease does not correlate with dynamic color doppler duplex ultrasound parameters: findings from a real-life cross-sectional study.","authors":"Alessandro Bertini, Edoardo Pozzi, Federico Belladelli, Massimiliano Raffo, Christian Corsini, Fausto Negri, Giacomo Musso, Francesco Cattafi, Riccardo Ramadani, Luigi Candela, Alessia d' Arma, Francesco Montorsi, Andrea Salonia","doi":"10.1111/andr.13771","DOIUrl":"https://doi.org/10.1111/andr.13771","url":null,"abstract":"<p><strong>Introduction: </strong>There is inconsistent data regarding the possible inaccuracies in dynamic penile color Doppler duplex ultrasound (CDDU) measurements in men with penile curvature because of Peyronie's disease (PD). We sought to explore the relationship between the degree of penile curvature and CDDU parameters in men with PD.</p><p><strong>Materials and methods: </strong>Comprehensive data from 154 consecutive men presenting with PD as their primary complaint at a single academic center were prospectively collected and analyzed. All men underwent CDDU. Penile curvature was measured using a goniometer at time of maximum erection during CDDU. Patients were grouped based on CDDU parameters into-normal (average peak systolic velocity [PSV] ≥ 35 cm/s and resistance index [RI] ≥ 0.85) and pathological CDDU (average PSV < 35 cm/s and/or RI < 0.85). Descriptive statistics was used to compare the two subcohorts. Linear regression models were fitted to explore the association between the degree of penile curvature and dynamic CDDU parameters.</p><p><strong>Results: </strong>Overall, the median interquartile range (IQR) age was 56 (48-63) years. The median (IQR) PSV and degree of penile curvature were 48.8 cm/s (37.9-58.5) and 40 degrees (30-60), respectively. At CDDU, the degrees of penile curvature were as follows: 10-30 degrees in 63 (40.9%) men, 30-70 degrees in 70 (45.5%) men, and 70-90 degrees in 21 (13.6%) men, respectively. Of all, 116 (75.3%) patients showed a PSV > 35 cm/s and RI ≥ 0.85. Patients with pathologic vs. normal CDDU parameters did not differ in median (IQR) curvature (32.5° [30°-58.7°] vs. 40° [30°-65°], p = 0.5) or in the distribution across curvature range groups. Linear regression analysis revealed that the degree of penile curvature did not significantly correlate with PSV at CDDU (coefficient: 0.06, p = 0.3).</p><p><strong>Conclusions: </strong>Our study confirms the lack of a significant correlation between the severity of penile curvature and CDDU parameters in men presenting with PD. These findings emerge to be relevant in terms of a more accurate management work-up for PD patients and hold insightful medicolegal implications and in the real-life setting.</p>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142339545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ascorbic acid 2-glucoside improves survival, quality, and fertility of frozen-thawed C57Bl/6J and C57Bl/6N mouse spermatozoa. 抗坏血酸 2-葡萄糖苷可提高冷冻解冻的 C57Bl/6J 和 C57Bl/6N 小鼠精子的存活率、质量和生育能力。
IF 3.2 2区 医学 Q1 ANDROLOGY Pub Date : 2024-09-27 DOI: 10.1111/andr.13768
Marcello Raspa, Renata Paoletti, Ferdinando Scavizzi

Background: Ascorbic acid 2-glucoside (AA2G) is a stabilized form of ascorbic acid and a potent antioxidant. Ascorbic acid is present in the testes and epididymis and helps maintain the physiological integrity of reproductive organs. Its properties have been utilized to protect spermatozoa of different species from oxidative stress.

Materials and methods: Spermatozoa of C57Bl/6J and C57Bl/6N strains were frozen and analyzed, after thawing, by supplementing the capacitation medium with AA2G, both in the presence and absence of methyl-β-cyclodextrin (MBCD). The effect of treatment was evaluated by SCA System (Microptic) analyzing the velocity, vitality, morphology, and the DNA fragmentation. We also examined sperm capacitation (CTC), acrosome reaction (Coomassie Brillant Blue), and fertility (in vitro fertilization) of treated spermatozoa.

Results: AA2G improved sperm quality and fertility particularly in association with MBCD. We observed a significant increase of sperm motility, velocity, and vitality associated with an enhanced capacitation and acrosome reaction. These improvements resulted in a marked increase in in vitro fertilization success. Embryos obtained were cultured and reached normally the blastocyst stage.

Discussion: This study aimed to determine if AA2G could safeguard mouse spermatozoa during cryopreservation. We found a protective effect of AA2G that increased sperm survivability resulting in higher fertilization rate.

Conclusion: This newly improved protocol shows potential for reanimating cryopreserved GEMMs stored in mouse biobanks and international repositories, such as the European Mouse Mutant Archive (EMMA). This can serve as a pivotal tool in fulfilling the 3Rs mission (replacement, reduction, and refinement), promoting ethical and humane research practices.

背景:抗坏血酸 2-葡萄糖苷(AA2G)是抗坏血酸的一种稳定形式,也是一种有效的抗氧化剂。抗坏血酸存在于睾丸和附睾中,有助于维持生殖器官的生理完整性。抗坏血酸的特性已被用于保护不同物种的精子免受氧化应激:冷冻 C57Bl/6J 和 C57Bl/6N 株系的精子,解冻后在有甲基-β-环糊精(MBCD)或没有甲基-β-环糊精(MBCD)的情况下,在获能培养基中添加 AA2G 进行分析。处理效果由 SCA 系统(Microptic)进行评估,分析速度、活力、形态和 DNA 片段。我们还检测了经处理精子的获能能力(CTC)、顶体反应(Coomassie Brillant Blue)和受精能力(体外受精):结果:AA2G 提高了精子质量和受精率,尤其是在与中性粒细胞生长因子(MBCD)联合使用时。我们观察到精子的运动能力、速度和活力明显提高,获能能力和顶体反应增强。这些改善显著提高了体外受精的成功率。获得的胚胎经培养后通常可达到囊胚期:本研究旨在确定 AA2G 能否在冷冻保存过程中保护小鼠精子。我们发现 AA2G 有保护精子的作用,它能提高精子的存活率,从而提高受精率:结论:这一新近改进的方案显示出重新激活冷冻保存在小鼠生物库和国际储存库(如欧洲小鼠突变体档案库(EMMA))中的 GEMMs 的潜力。这可以作为实现 3R 任务(替换、减少和改进)的关键工具,促进符合伦理和人道的研究实践。
{"title":"Ascorbic acid 2-glucoside improves survival, quality, and fertility of frozen-thawed C57Bl/6J and C57Bl/6N mouse spermatozoa.","authors":"Marcello Raspa, Renata Paoletti, Ferdinando Scavizzi","doi":"10.1111/andr.13768","DOIUrl":"https://doi.org/10.1111/andr.13768","url":null,"abstract":"<p><strong>Background: </strong>Ascorbic acid 2-glucoside (AA2G) is a stabilized form of ascorbic acid and a potent antioxidant. Ascorbic acid is present in the testes and epididymis and helps maintain the physiological integrity of reproductive organs. Its properties have been utilized to protect spermatozoa of different species from oxidative stress.</p><p><strong>Materials and methods: </strong>Spermatozoa of C57Bl/6J and C57Bl/6N strains were frozen and analyzed, after thawing, by supplementing the capacitation medium with AA2G, both in the presence and absence of methyl-β-cyclodextrin (MBCD). The effect of treatment was evaluated by SCA System (Microptic) analyzing the velocity, vitality, morphology, and the DNA fragmentation. We also examined sperm capacitation (CTC), acrosome reaction (Coomassie Brillant Blue), and fertility (in vitro fertilization) of treated spermatozoa.</p><p><strong>Results: </strong>AA2G improved sperm quality and fertility particularly in association with MBCD. We observed a significant increase of sperm motility, velocity, and vitality associated with an enhanced capacitation and acrosome reaction. These improvements resulted in a marked increase in in vitro fertilization success. Embryos obtained were cultured and reached normally the blastocyst stage.</p><p><strong>Discussion: </strong>This study aimed to determine if AA2G could safeguard mouse spermatozoa during cryopreservation. We found a protective effect of AA2G that increased sperm survivability resulting in higher fertilization rate.</p><p><strong>Conclusion: </strong>This newly improved protocol shows potential for reanimating cryopreserved GEMMs stored in mouse biobanks and international repositories, such as the European Mouse Mutant Archive (EMMA). This can serve as a pivotal tool in fulfilling the 3Rs mission (replacement, reduction, and refinement), promoting ethical and humane research practices.</p>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142339543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Testicular fibrosis pathology, diagnosis, pathogenesis, and treatment: A perspective on related diseases. 睾丸纤维化的病理、诊断、发病机制和治疗:相关疾病透视。
IF 3.2 2区 医学 Q1 ANDROLOGY Pub Date : 2024-09-27 DOI: 10.1111/andr.13769
Ying Xu, Poyi Hu, Wanyi Chen, Jin Chen, Chunyan Liu, Huiping Zhang

Testicular fibrosis is a chronic and progressive condition characterized by the excessive deposition of extracellular matrix proteins. This process leads to fibrotic remodeling, damage to testicular tissue, and the irreversible loss of male reproductive function. However, there is currently a lack of comprehensive reviews systematically elucidating the pathology, diagnosis, pathogenesis, and treatment of testicular fibrosis from the perspectives of different related diseases. This review addresses these aspects of testicular fibrosis, with a particular emphasis on elucidating the underlying mechanisms of testicular cells. It provides insights that can be relevant for future research and clinical interventions.

睾丸纤维化是一种慢性进行性疾病,其特点是细胞外基质蛋白过度沉积。这一过程会导致纤维化重塑、睾丸组织受损以及男性生殖功能不可逆转的丧失。然而,目前还缺乏全面的综述,从不同相关疾病的角度系统地阐明睾丸纤维化的病理、诊断、发病机制和治疗。本综述探讨了睾丸纤维化的这些方面,尤其侧重于阐明睾丸细胞的内在机制。它为未来的研究和临床干预提供了相关的见解。
{"title":"Testicular fibrosis pathology, diagnosis, pathogenesis, and treatment: A perspective on related diseases.","authors":"Ying Xu, Poyi Hu, Wanyi Chen, Jin Chen, Chunyan Liu, Huiping Zhang","doi":"10.1111/andr.13769","DOIUrl":"https://doi.org/10.1111/andr.13769","url":null,"abstract":"<p><p>Testicular fibrosis is a chronic and progressive condition characterized by the excessive deposition of extracellular matrix proteins. This process leads to fibrotic remodeling, damage to testicular tissue, and the irreversible loss of male reproductive function. However, there is currently a lack of comprehensive reviews systematically elucidating the pathology, diagnosis, pathogenesis, and treatment of testicular fibrosis from the perspectives of different related diseases. This review addresses these aspects of testicular fibrosis, with a particular emphasis on elucidating the underlying mechanisms of testicular cells. It provides insights that can be relevant for future research and clinical interventions.</p>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142339546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A homozygous nonsense variant in HENMT1 causes male infertility in humans and mice. HENMT1 的同卵无义变体会导致人类和小鼠雄性不育。
IF 3.2 2区 医学 Q1 ANDROLOGY Pub Date : 2024-09-25 DOI: 10.1111/andr.13767
Ming Li, Tanveer Abbas, Yue Wang, Aoran Zhi, Jianteng Zhou, Ao Ma, Ghulam Murtaza, Yufan Wu, Wasim Shah, Muhammad Zubair, Muzammil Ahmad Khan, Furhan Iqbal, Xiaohua Jiang, Huan Zhang, Qinghua Shi

Background: HENMT1 encodes a small RNA methyltransferase that plays a crucial role in mouse spermatogenesis through the methylation of the 3' end of PIWI-interacting RNAs.

Objectives: Our study aims to elucidate the relationship between HENMT1 and male infertility in humans.

Materials and methods: A consanguineous family, having a single non-obstructive azoospermia patient was recruited for pathogenic variants screening. The research includes genetic analysis and experimental validation using mouse models. The patient was diagnosed with non-obstructive azoospermia. Whole-exome sequencing and subsequent bioinformatic analyses were performed to screen for candidate pathogenic variants. The pathogenicity of the identified variant was assessed and studied in vivo using a mouse model that mimicked the patient's mutation.

Results: Through whole-exome sequencing, we identified a homozygous nonsense variant (c.555G > A, p.Trp185*) in HENMT1 in the patient. The presence of the mutant HENMT1 mRNA was detected in the patient's blood, and the truncated HENMT1 protein was observed in transfected HEK293T cells. The mutant mice modeling this HENMT1 variant displayed an infertile phenotype similar to that of the patient, characterized by spermiogenesis arrest. Further analysis revealed a significant derepression of retrotransposon LINE1 in the testes of the Henmt1 mutant mice, and increased apoptosis of spermatids.

Discussion and conclusion: Our findings provide the evidence of pathogenicity of the identified HENMT1 variant, thus shedding light on the indispensable role of HENMT1 in human spermatogenesis.

背景:HENMT1编码一种小RNA甲基转移酶,它通过甲基化PIWI相互作用RNA的3'端,在小鼠精子发生过程中发挥关键作用:我们的研究旨在阐明HENMT1与人类男性不育的关系:材料和方法:我们招募了一个有一名非梗阻性无精子症患者的近亲家庭进行致病变体筛选。研究包括遗传分析和使用小鼠模型进行实验验证。该患者被确诊为非梗阻性无精子症。研究人员进行了全外显子组测序和随后的生物信息学分析,以筛选候选致病变体。利用模仿患者变异的小鼠模型,对所发现变异的致病性进行了体内评估和研究:结果:通过全外显子组测序,我们确定了患者体内 HENMT1 的一个同卵无义变体(c.555G > A, p.Trp185*)。在患者的血液中检测到了突变的 HENMT1 mRNA,在转染的 HEK293T 细胞中观察到了截短的 HENMT1 蛋白。以这种 HENMT1 变体为模型的突变小鼠表现出与患者类似的不育表型,其特点是精子发生停滞。进一步的分析表明,Henmt1 突变小鼠睾丸中的逆转录质子 LINE1 明显减弱,精子凋亡增加:我们的研究结果为HENMT1变异体的致病性提供了证据,从而揭示了HENMT1在人类精子发生过程中不可或缺的作用。
{"title":"A homozygous nonsense variant in HENMT1 causes male infertility in humans and mice.","authors":"Ming Li, Tanveer Abbas, Yue Wang, Aoran Zhi, Jianteng Zhou, Ao Ma, Ghulam Murtaza, Yufan Wu, Wasim Shah, Muhammad Zubair, Muzammil Ahmad Khan, Furhan Iqbal, Xiaohua Jiang, Huan Zhang, Qinghua Shi","doi":"10.1111/andr.13767","DOIUrl":"https://doi.org/10.1111/andr.13767","url":null,"abstract":"<p><strong>Background: </strong>HENMT1 encodes a small RNA methyltransferase that plays a crucial role in mouse spermatogenesis through the methylation of the 3' end of PIWI-interacting RNAs.</p><p><strong>Objectives: </strong>Our study aims to elucidate the relationship between HENMT1 and male infertility in humans.</p><p><strong>Materials and methods: </strong>A consanguineous family, having a single non-obstructive azoospermia patient was recruited for pathogenic variants screening. The research includes genetic analysis and experimental validation using mouse models. The patient was diagnosed with non-obstructive azoospermia. Whole-exome sequencing and subsequent bioinformatic analyses were performed to screen for candidate pathogenic variants. The pathogenicity of the identified variant was assessed and studied in vivo using a mouse model that mimicked the patient's mutation.</p><p><strong>Results: </strong>Through whole-exome sequencing, we identified a homozygous nonsense variant (c.555G > A, p.Trp185*) in HENMT1 in the patient. The presence of the mutant HENMT1 mRNA was detected in the patient's blood, and the truncated HENMT1 protein was observed in transfected HEK293T cells. The mutant mice modeling this HENMT1 variant displayed an infertile phenotype similar to that of the patient, characterized by spermiogenesis arrest. Further analysis revealed a significant derepression of retrotransposon LINE1 in the testes of the Henmt1 mutant mice, and increased apoptosis of spermatids.</p><p><strong>Discussion and conclusion: </strong>Our findings provide the evidence of pathogenicity of the identified HENMT1 variant, thus shedding light on the indispensable role of HENMT1 in human spermatogenesis.</p>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142339542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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Andrology
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