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Cell vision : the journal of analytical morphology最新文献

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Introduction to the genetics of cancer. 介绍癌症的遗传学。
Cell vision : the journal of analytical morphology
Pub Date : 1998-01-01
J M Elias
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引用次数: 0
Flow cytometry: an overview. 流式细胞术:概述。
Cell vision : the journal of analytical morphology
Pub Date : 1998-01-01
B H Villas

Flow cytometry is an innovative technology that measures certain cell parameters as the cells flow in a fluid stream and in single file past an analytical laser light source. Clinical applications of flow cytometry currently utilized in the laboratory include cell surface antigen determinations or immunophenotyping of hematologic cells, DNA analysis of hematopoietic malignancies and solid tumors, and measurement of CD4 (T helper/inducer cell) absolute counts and T helper/T suppressor (CD4/CD8) ratios in the evaluation of immune deficiency. Flow cytometry often offers a more rapid, sensitive, accurate, and quantitative means of analyzing a particular cell population in a heterogeneous cell suspension as compared to more traditional microscopic methods. This article is intended to provide a general understanding of the technological basis of how a flow cytometer functions as well as an overview of both current and future flow cytometric clinical laboratory applications.

流式细胞术是一项创新技术，当细胞在流体中流动时，它可以测量细胞的某些参数，并在单个队列中通过分析激光光源。流式细胞术目前在实验室的临床应用包括血液细胞的细胞表面抗原测定或免疫表型分型，造血恶性肿瘤和实体瘤的DNA分析，以及免疫缺陷评价中CD4 (T辅助/诱导细胞)绝对计数和T辅助/T抑制(CD4/CD8)比值的测定。与传统的显微方法相比，流式细胞术通常提供了一种更快速、灵敏、准确和定量的方法来分析异质细胞悬液中的特定细胞群。本文旨在提供流式细胞仪功能的技术基础的一般理解以及当前和未来流式细胞仪临床实验室应用的概述。
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引用次数: 0
Detection of mRNA in tissue sections by in situ RT-PCR. 原位RT-PCR检测组织切片mRNA。
Cell vision : the journal of analytical morphology
Pub Date : 1998-01-01
A Martinez
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引用次数: 0
RT in situ PCR for the detection of mRNA transcripts of Fas-L in the immune-privileged placental environment. RT原位PCR检测免疫特权胎盘环境中Fas-L mRNA转录物。
Cell vision : the journal of analytical morphology
Pub Date : 1998-01-01
A Steele, D Uckan, P Steele, W Chamizo, K Washington, A Koutsonikolis, R A Good

Rapid and reproducible detection of RNA in cells and tissue sections is routinely accomplished using in-situ hybridization technique provided that the target number of mRNA copies is above a minimum number. Detection of low copy transcripts is problematic when threshold detection occurs below clear signal resolution or alternatively, when technical problems result in background noise which occludes clear signal. RT in-situ PCR methodology utilizes both the power and specificity of PCR to amplify target whose localization is subsequently detected at the cellular level. RT in-situ PCR methods routinely involve a two-step methodology. mRNA copies are initially transcribed into cDNA. This step is followed by a separate PCR step wherein amplification of the newly synthesized cDNA takes place. A simplified one-step procedure biochemically compartmentalizes these sequential steps within a single applications methodology using the enzyme rTth. This method was successfully applied to detect and localize mRNA transcripts for Fas ligand within the immune privileged placental environment and to provide verification of immunohistochemical localization of gene product.

在细胞和组织切片中快速和可重复的RNA检测通常使用原位杂交技术完成，前提是mRNA的目标拷贝数高于最小数量。当阈值检测低于清晰信号分辨率时，或者当技术问题导致背景噪声遮挡清晰信号时，低拷贝转录本的检测就会出现问题。RT原位PCR方法利用PCR的能力和特异性来扩增目标，随后在细胞水平上检测其定位。RT原位PCR方法通常包括两步方法。mRNA拷贝最初转录成cDNA。这一步之后是一个单独的PCR步骤，其中新合成的cDNA进行扩增。一个简化的一步程序在使用酶th的单一应用方法中生物化学地划分这些顺序步骤。该方法成功地用于检测和定位免疫特权胎盘环境中Fas配体的mRNA转录本，并为基因产物的免疫组织化学定位提供了验证。
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引用次数: 0
Ultrastructural observations on the cells of a patient with Fanconi's anemia transforming to acute leukemia. 范可尼贫血转化为急性白血病的细胞超微结构观察。
Cell vision : the journal of analytical morphology
Pub Date : 1998-01-01
R Straussberg, H Bessler, S Alexandrova, H Salman, M Bergman, M Djaldetti

The ultrastructural findings in the bone marrow and peripheral blood cells of a patient with Fanconi's anemia transforming to acute myelo-monocytic leukemia are presented. They consisted of an unusual large number of nuclear pockets, bridges and appendices observed in the nuclei of the polymorphonuclear cells, monocytes and to a lesser extent in the mature normoblasts. Since the mother and two younger brothers of the propositus were found to have macrocytic anemia and pancytopenia, most probably Fanconi's anemia, their peripheral blood cells were also examined with the electron microscope. The cells of all of them showed similar nuclear alterations, although in lesser quantity. The possibility that these findings are consistent with the diagnosis of Fanconi's anemia, or they herald an evolution of the disease to acute leukemia is considered.

本文报道一例范可尼贫血转化为急性髓单核细胞白血病的患者骨髓和外周血细胞超微结构的变化。在多形核细胞、单核细胞的细胞核中观察到异常多的核袋、核桥和核附件，在成熟的正母细胞中也有少量观察到。由于母亲和两个弟弟被发现患有巨细胞性贫血和全血细胞减少症，很可能是范可尼贫血，他们的外周血也被电子显微镜检查了。所有的细胞都表现出类似的细胞核改变，尽管数量较少。这些发现可能与范可尼贫血的诊断一致，或者它们预示着该疾病向急性白血病的发展。
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引用次数: 0
Highly sensitive DNA, RNA and antigen detection methods: streptavidin-Nanogold-silver staining. 高灵敏度的DNA、RNA和抗原检测方法:链霉亲和素-纳米金-银染色。
Cell vision : the journal of analytical morphology
Pub Date : 1998-01-01
C Hauser-Kronberger
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引用次数: 0
Comparison of HBV DNA amplification with PCR in patients with serological evidence of HBV infection--a report from India. HBV DNA扩增与PCR在血清学证据为HBV感染的患者中的比较——一份来自印度的报告。
Cell vision : the journal of analytical morphology
Pub Date : 1998-01-01
T Jacob, C Rodrigues, S Menon, R Nukala, A Mehta
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引用次数: 0
Detection of telomerase activity in microdissected breast lesions. 微解剖乳腺病变端粒酶活性的检测。
Cell vision : the journal of analytical morphology
Pub Date : 1998-01-01
Y G Man, D P Schammel, F A Tavassoli
{"title":"Detection of telomerase activity in microdissected breast lesions.","authors":"Y G Man,&nbsp;D P Schammel,&nbsp;F A Tavassoli","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":79574,"journal":{"name":"Cell vision : the journal of analytical morphology","volume":"5 1","pages":"84"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20577992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
High grade malignant fibrous histiocytomas have bimodal cycling populations of factor XIIIa+ dendrophages and dedifferentiated mesenchymal cells possibly derived from CD34+ fibroblasts. 高级别恶性纤维组织细胞瘤具有双峰循环的因子XIIIa+树突细胞和可能来源于CD34+成纤维细胞的去分化间充质细胞群。
Cell vision : the journal of analytical morphology
Pub Date : 1998-01-01
J S Silverman, A Tamsen
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引用次数: 0
Observations on the role of FXIIIa+ dendritic mammary stromal cells in myofibroblastic stromal remodeling and angiogenesis in mammary carcinoma. FXIIIa+树突状乳腺基质细胞在乳腺癌肌成纤维基质重塑和血管生成中的作用观察。
Cell vision : the journal of analytical morphology
Pub Date : 1998-01-01
J S Silverman, A Tamsen
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引用次数: 0
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