Pub Date : 2021-08-04DOI: 10.26420/annagriccropsci.2021.1090
Dallas Rj, da Silva Mhm, V. L., D. Fm
Boar semen cryopreservation has a high potential in the swine industry, allowing the large-scale use of genetically superior animals, improving efficiency, product quality, helping to reduce the risk of disease spread and gathering needs from the market. From a genetic point of view, semen freezing is desirable for genetic diversification, favouring a more efficient reproduction as well as the constitution of germplasm banks, including for repopulation in case of disease outbreak. However, freezing this semen for long periods for practical use is limited by the reduced viability and fertilization potential caused to sperm during the cryopreservation process and consequently low conception rates and smaller litters after artificial insemination. In part, the decrease in the fertilizing power of frozen spermatozoa may be associated with oxidative damage due to excessive formation of Reactive Oxygen Species (ROS), osmotic stress and cell damage due to ice formation during cryopreservation. To suppress the damage caused by ROS, the present study was conducted to determine the impact of supplementation with three antioxidants, these being ascorbic acid, a-tocopherol and reduced glutathione, evaluating the parameters of semen quality, viability, total and progressive motility, vigour and agglutination rate after thawing. For this purpose, semen was collected from five boars, each being collected three times, at weekly intervals, always at the same time. Immediately after harvesting, the macroscopic (colour, appearance, and volume) and microscopic evaluation of the semen (mass motility, concentration, progressive individual motility, spermatic vigour and spermatic morphology) were evaluated. Subsequently, the semen was placed at 15°C for two hours and centrifuged at 800 x g for 10 minutes also at 15°C, removing the supernatant. For the freezing medium, a base medium consisting of a commercial MR-A extender, supplemented with 3% v/v glycerol, 10% v/v egg yolk and 0.20% w/v Sodium Dodecyl Sulfate (SDS) was used. The nine treatments used in the study were, respectively, ascorbic acid at concentrations of 100, 200 and 400μL, a-Tocopherol at concentrations of 200, 400 and 800μM and reduced Glutathione at concentrations of 2.5, 5 and 10 mg/l and numbered as T1 to T9, respectively. In the control group, semen was frozen in a medium without adding any antioxidant. The semen belonging to the different treatments and to the control was placed in 0.25ml insemination French straws and incubated at 6°C for two hours. The subsequent freezing was carried out in nitrogen vapours (-120°C) for ten minutes and immersed in liquid nitrogen after this period. After 7 days, the semen was thawed in a water bath at 37°C for 20 seconds, the straws dried on paper, placed on a microscope slide heated to 37°C and evaluated according to the parameters described above. Regarding the comparison between the different treatments, it was observed that the sperm viability obtained in the
{"title":"Effect of Antioxidants on Pig Semen Cryopreservation to Preserve Sperm Fertility after Thawing","authors":"Dallas Rj, da Silva Mhm, V. L., D. Fm","doi":"10.26420/annagriccropsci.2021.1090","DOIUrl":"https://doi.org/10.26420/annagriccropsci.2021.1090","url":null,"abstract":"Boar semen cryopreservation has a high potential in the swine industry, allowing the large-scale use of genetically superior animals, improving efficiency, product quality, helping to reduce the risk of disease spread and gathering needs from the market. From a genetic point of view, semen freezing is desirable for genetic diversification, favouring a more efficient reproduction as well as the constitution of germplasm banks, including for repopulation in case of disease outbreak. However, freezing this semen for long periods for practical use is limited by the reduced viability and fertilization potential caused to sperm during the cryopreservation process and consequently low conception rates and smaller litters after artificial insemination. In part, the decrease in the fertilizing power of frozen spermatozoa may be associated with oxidative damage due to excessive formation of Reactive Oxygen Species (ROS), osmotic stress and cell damage due to ice formation during cryopreservation. To suppress the damage caused by ROS, the present study was conducted to determine the impact of supplementation with three antioxidants, these being ascorbic acid, a-tocopherol and reduced glutathione, evaluating the parameters of semen quality, viability, total and progressive motility, vigour and agglutination rate after thawing. For this purpose, semen was collected from five boars, each being collected three times, at weekly intervals, always at the same time. Immediately after harvesting, the macroscopic (colour, appearance, and volume) and microscopic evaluation of the semen (mass motility, concentration, progressive individual motility, spermatic vigour and spermatic morphology) were evaluated. Subsequently, the semen was placed at 15°C for two hours and centrifuged at 800 x g for 10 minutes also at 15°C, removing the supernatant. For the freezing medium, a base medium consisting of a commercial MR-A extender, supplemented with 3% v/v glycerol, 10% v/v egg yolk and 0.20% w/v Sodium Dodecyl Sulfate (SDS) was used. The nine treatments used in the study were, respectively, ascorbic acid at concentrations of 100, 200 and 400μL, a-Tocopherol at concentrations of 200, 400 and 800μM and reduced Glutathione at concentrations of 2.5, 5 and 10 mg/l and numbered as T1 to T9, respectively. In the control group, semen was frozen in a medium without adding any antioxidant. The semen belonging to the different treatments and to the control was placed in 0.25ml insemination French straws and incubated at 6°C for two hours. The subsequent freezing was carried out in nitrogen vapours (-120°C) for ten minutes and immersed in liquid nitrogen after this period. After 7 days, the semen was thawed in a water bath at 37°C for 20 seconds, the straws dried on paper, placed on a microscope slide heated to 37°C and evaluated according to the parameters described above. Regarding the comparison between the different treatments, it was observed that the sperm viability obtained in the ","PeriodicalId":8133,"journal":{"name":"Annals of Agricultural & Crop Sciences","volume":"40 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73867864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-30DOI: 10.26420/annagriccropsci.2021.1089
N. M, H. Hs, Yasir Qm, D. H, Satti Z
Cotton is an excessive growing fiber crop in the world. In Pakistan, climate warming is hurting the cotton crop. To identify countermeasures of climate change impacts on crop production, it is needed to explore the changes in crop yield and their relationship to climate change. This study, we conducted spatial analysis of cotton yield and climate data in the Indus River basin in Pakistan from 1989- 2018. We analyzed the trend in cotton production indices and climate variables using the Mann-Kendal nonparametric test, and the impact of climate change on cotton yield through Pearson’s correlation and best-subset multivariate linear regression model. Sunshine hours positively impacted on cotton yield, but an increasing trend in average temperature, minimum temperature and air relative humidity in main crop areas had a negative impact on the cotton crop. Our results illustrate the relationship between the cotton yield and climate change variables on regional basis in the Indus River basin, Pakistan. This research could provide a reference for understanding the intolerance of cotton in the Indus River basin, Pakistan to climate change.
{"title":"Analyzing the Impact of Climate Change on Cotton Yield Using Spatial Analysis and Statistical Modeling in the Indus River Basin, Pakistan","authors":"N. M, H. Hs, Yasir Qm, D. H, Satti Z","doi":"10.26420/annagriccropsci.2021.1089","DOIUrl":"https://doi.org/10.26420/annagriccropsci.2021.1089","url":null,"abstract":"Cotton is an excessive growing fiber crop in the world. In Pakistan, climate warming is hurting the cotton crop. To identify countermeasures of climate change impacts on crop production, it is needed to explore the changes in crop yield and their relationship to climate change. This study, we conducted spatial analysis of cotton yield and climate data in the Indus River basin in Pakistan from 1989- 2018. We analyzed the trend in cotton production indices and climate variables using the Mann-Kendal nonparametric test, and the impact of climate change on cotton yield through Pearson’s correlation and best-subset multivariate linear regression model. Sunshine hours positively impacted on cotton yield, but an increasing trend in average temperature, minimum temperature and air relative humidity in main crop areas had a negative impact on the cotton crop. Our results illustrate the relationship between the cotton yield and climate change variables on regional basis in the Indus River basin, Pakistan. This research could provide a reference for understanding the intolerance of cotton in the Indus River basin, Pakistan to climate change.","PeriodicalId":8133,"journal":{"name":"Annals of Agricultural & Crop Sciences","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83369940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-08DOI: 10.26420/annagriccropsci.2021.1087
R. Darai, Coordinator, A. Sarker, Pandey Mp, A. Sk, Thapa Db, Dhakal Kh, R. Coordinator, Nasc Complex New Delhi India China Program
Lentil (Lens culinaris Medikus subsp. culinaris) is the wonderful, most popular & preferred food legume of Nepalese people and commonly grown in the rice and maize based cropping system. Morphological characters have been frequently used in order to know the diversity in germplasm collections. The main objectives of the study were to characterize the bio-fortified lentil accessions using morphological markers and select the DUS accession for using targeted the best utilization in hybridization program. Plant breeders can use these morphological variations to make decision regarding the choice for selecting superior genotypes for improvement or to be utilized as parents for the development of future cultivars through hybridization. Furthermore, important morphological markers like, plant type, foliage color, testa color, testa pattern and cotyledon colors can also be used for testing hybridity and keeping genetic purity at genetic level. The information obtained by the identification keys at seed and plant levels may be useful for discrimination and verification of varieties, hybridity testing and maintenance of genetic purity at genetic level during seed production and certification programme. Out of 25 lentil accessions, 18 accessions were observed as erect and compact growth habit, 22 accessions had green stem color, 21 accessions had dense leaf pubescence and dark foliage, 7 accessions had prominent tendril, 3 accessions had white blue veins flower and 4 accessions had large seeded accession.
{"title":"Morphological Characterization of High Grain Iron and Zinc Concentration Bio-fortified lentil (Lens culinaris Medikus subsp. culinaris) in Nepal","authors":"R. Darai, Coordinator, A. Sarker, Pandey Mp, A. Sk, Thapa Db, Dhakal Kh, R. Coordinator, Nasc Complex New Delhi India China Program","doi":"10.26420/annagriccropsci.2021.1087","DOIUrl":"https://doi.org/10.26420/annagriccropsci.2021.1087","url":null,"abstract":"Lentil (Lens culinaris Medikus subsp. culinaris) is the wonderful, most popular & preferred food legume of Nepalese people and commonly grown in the rice and maize based cropping system. Morphological characters have been frequently used in order to know the diversity in germplasm collections. The main objectives of the study were to characterize the bio-fortified lentil accessions using morphological markers and select the DUS accession for using targeted the best utilization in hybridization program. Plant breeders can use these morphological variations to make decision regarding the choice for selecting superior genotypes for improvement or to be utilized as parents for the development of future cultivars through hybridization. Furthermore, important morphological markers like, plant type, foliage color, testa color, testa pattern and cotyledon colors can also be used for testing hybridity and keeping genetic purity at genetic level. The information obtained by the identification keys at seed and plant levels may be useful for discrimination and verification of varieties, hybridity testing and maintenance of genetic purity at genetic level during seed production and certification programme. Out of 25 lentil accessions, 18 accessions were observed as erect and compact growth habit, 22 accessions had green stem color, 21 accessions had dense leaf pubescence and dark foliage, 7 accessions had prominent tendril, 3 accessions had white blue veins flower and 4 accessions had large seeded accession.","PeriodicalId":8133,"journal":{"name":"Annals of Agricultural & Crop Sciences","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76163871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-25DOI: 10.26420/annagriccropsci.2021.1085
U. Sakthivel
The suitability of biomass system depends on products recovery from the waste. Switchgrass was the most renewable biomass sources and selected as feedstock for the volatile fatty acid production from anaerobic digestion. The five kinds of pretreatments involving physical and chemical treatment such as thermal, hot water, acid, alkaline and organosolv pretreatment were investigated. This study explored the characteristics of Volatile Fatty Acid (VFA) production from pretreated switchgrass compared with raw biomass. The major VFA compounds are acetic acid, propionic acid, butyric acid, iso-butyric acid and iso-valeric acid and hexanoic acid produced during digestion. The result showed that acetic acid concentration yields higher with other compounds of VFA.
{"title":"Bio-Valorization of Physical and Chemical Pretreated Switchgrass on Volatile Fatty Acid Production","authors":"U. Sakthivel","doi":"10.26420/annagriccropsci.2021.1085","DOIUrl":"https://doi.org/10.26420/annagriccropsci.2021.1085","url":null,"abstract":"The suitability of biomass system depends on products recovery from the waste. Switchgrass was the most renewable biomass sources and selected as feedstock for the volatile fatty acid production from anaerobic digestion. The five kinds of pretreatments involving physical and chemical treatment such as thermal, hot water, acid, alkaline and organosolv pretreatment were investigated. This study explored the characteristics of Volatile Fatty Acid (VFA) production from pretreated switchgrass compared with raw biomass. The major VFA compounds are acetic acid, propionic acid, butyric acid, iso-butyric acid and iso-valeric acid and hexanoic acid produced during digestion. The result showed that acetic acid concentration yields higher with other compounds of VFA.","PeriodicalId":8133,"journal":{"name":"Annals of Agricultural & Crop Sciences","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82079991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-25DOI: 10.26420/annagriccropsci.2021.1086
Dalvi Sunil G
Sugarcane is an important cash crop gaining importance as ideal raw material for bioenergy production. Conventional sugarcane improvement is carried out through intensive crossing and selection breeding within the Saccharum complex. Being highly polyploidy crop the mutation breeding is also integrated for specifically improving the desired genetic trait in sugarcane varieties developed through conventional breeding. In the present work RAPD markers based assessment of genetic diversity and kinship relationships of six promising mutants developed using CoC 671 has been reported. The banding pattern of the PCR amplified products showed polymorphism with the RAPD profile in all six mutants. Mutants CoC 671, TC 2813, TC 2819, TC 2826, TC 2875 formed a separate cluster, and TC 906, TC 922, and TC 906 B formed a separate cluster distinct from the earlier one with the parent (CoC 671) and the standard Check (Co 86023). The genetic distance between the groups was found to be only marginally higher than the respective group distances and the overall mean genetic distance. The similarity indices indicated that there was 15 % genetic dissimilarity between CoC 671 and Co 86032. This may be because CoC 671 was one of the parents for Co 86032. In the present investigation, the genetic similarity value ranged from 0.97 to 0.78 among the mutants. A total of 34 band positions were scored, out of which 15 RAPD bands were polymorphic. From the genetic similarity coefficient based on RAPD band data sharing, it was found that the majority of the clones were almost more than 78% similar to the mother plant. Thus the level of similarity between varieties seemed to be not very high but the mutants could be distinguished from each other easily. RAPD analysis indicated EMS-induced point mutations resulting in specific rectifications without much change in the genetic backbone of genotype CoC 671. Since this analysis was carried out on mutants after multiplying in field for three successive generations, we presume that these mutants/genotypes are stable and express the stable genetic variations.
{"title":"Assessment of Genetic Diversity in Promising Sugarcane Mutants Developed Through Chemical Mutagenesis","authors":"Dalvi Sunil G","doi":"10.26420/annagriccropsci.2021.1086","DOIUrl":"https://doi.org/10.26420/annagriccropsci.2021.1086","url":null,"abstract":"Sugarcane is an important cash crop gaining importance as ideal raw material for bioenergy production. Conventional sugarcane improvement is carried out through intensive crossing and selection breeding within the Saccharum complex. Being highly polyploidy crop the mutation breeding is also integrated for specifically improving the desired genetic trait in sugarcane varieties developed through conventional breeding. In the present work RAPD markers based assessment of genetic diversity and kinship relationships of six promising mutants developed using CoC 671 has been reported. The banding pattern of the PCR amplified products showed polymorphism with the RAPD profile in all six mutants. Mutants CoC 671, TC 2813, TC 2819, TC 2826, TC 2875 formed a separate cluster, and TC 906, TC 922, and TC 906 B formed a separate cluster distinct from the earlier one with the parent (CoC 671) and the standard Check (Co 86023). The genetic distance between the groups was found to be only marginally higher than the respective group distances and the overall mean genetic distance. The similarity indices indicated that there was 15 % genetic dissimilarity between CoC 671 and Co 86032. This may be because CoC 671 was one of the parents for Co 86032. In the present investigation, the genetic similarity value ranged from 0.97 to 0.78 among the mutants. A total of 34 band positions were scored, out of which 15 RAPD bands were polymorphic. From the genetic similarity coefficient based on RAPD band data sharing, it was found that the majority of the clones were almost more than 78% similar to the mother plant. Thus the level of similarity between varieties seemed to be not very high but the mutants could be distinguished from each other easily. RAPD analysis indicated EMS-induced point mutations resulting in specific rectifications without much change in the genetic backbone of genotype CoC 671. Since this analysis was carried out on mutants after multiplying in field for three successive generations, we presume that these mutants/genotypes are stable and express the stable genetic variations.","PeriodicalId":8133,"journal":{"name":"Annals of Agricultural & Crop Sciences","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88969333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-17DOI: 10.26420/annagriccropsci.2021.1084
ElBasyoni Is
Stripe rust is one of the most devastating biotic stresses to cause grain yield losses in wheat. In the current study, 227 imported accessions, and six widely grown modern cultivars (Sids14, Sids12, Misr1, Misr2, Giza171, and Gimmiza9), were used. All plant materials were planted in the field and evaluated for stripe rust resistance and grain yield. Five Simple Sequence Repeats (SSR) markers Xpsp3000, Xbarc8, Xgwm419, Xwmc44, and Xbarc32, respectively, are associated with five essential stripe rust resistance genes Yr10, Yr15, Yr26, Yr29, and Yr59, were also used. The results indicated a highly positive and significant correlation between grain yield and stripe rust resistance. Furthermore, as the number of stripe rust resistance alleles increased, both grain yield and stripe rust resistance increased. Out of the 233 accessions used, 11 accessions were found to contain the five resistance genes. The identified resistant accessions could be used as a gene source to enhance stripe rust resistance in wheat breeding programs. SSR markers used in the current study effectively capture a substantial part of the phenotypic variation caused by stripe rust. Thus, these five markers could be used effectively in marker-assisted selection for stripe rust resistance.
{"title":"Impact of Stripe Rust Resistance Alleles on Wheat Grain Yield Using Landraces and Improved Accessions","authors":"ElBasyoni Is","doi":"10.26420/annagriccropsci.2021.1084","DOIUrl":"https://doi.org/10.26420/annagriccropsci.2021.1084","url":null,"abstract":"Stripe rust is one of the most devastating biotic stresses to cause grain yield losses in wheat. In the current study, 227 imported accessions, and six widely grown modern cultivars (Sids14, Sids12, Misr1, Misr2, Giza171, and Gimmiza9), were used. All plant materials were planted in the field and evaluated for stripe rust resistance and grain yield. Five Simple Sequence Repeats (SSR) markers Xpsp3000, Xbarc8, Xgwm419, Xwmc44, and Xbarc32, respectively, are associated with five essential stripe rust resistance genes Yr10, Yr15, Yr26, Yr29, and Yr59, were also used. The results indicated a highly positive and significant correlation between grain yield and stripe rust resistance. Furthermore, as the number of stripe rust resistance alleles increased, both grain yield and stripe rust resistance increased. Out of the 233 accessions used, 11 accessions were found to contain the five resistance genes. The identified resistant accessions could be used as a gene source to enhance stripe rust resistance in wheat breeding programs. SSR markers used in the current study effectively capture a substantial part of the phenotypic variation caused by stripe rust. Thus, these five markers could be used effectively in marker-assisted selection for stripe rust resistance.","PeriodicalId":8133,"journal":{"name":"Annals of Agricultural & Crop Sciences","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90684442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-15DOI: 10.26420/annagriccropsci.2021.1083
Tuan Ntq, Hue Nh, Khuong Nq, Huu Tn, Thuc Lv
To improve the yield of sesame in Mekong delta of Vietnam, the study was conducted to find out plant growth regulator, micronutrient to increase growth and yield of black sesame. The experiment consisted of six treatments (NT) as follows: NT1: Control as no plant growth regulators and micronutrients, NT2: Spraying brassinolide at 50ppm, NT3: Spraying gibberellin at 100ppm and manganese at 0.05%, NT4: Spraying brassinolide at 1.25ppm, NT5: Spraying boric at 50ppm and molybdenum at 50ppm, NT6: Spraying selenium at 4ppm. The results showed that spraying plant growth regulators and micronutrients as single NAA, BR, Se and combined GA3 plus manganese, boric plus molybdenum at 27 and 35 days after sowing have not increased plant height and height to the first fruit in the first crop. Combination of spraying boric plus molybdenum or single selenium increased the number of capsules per plant, the number of seeds per capsule and resulted in higher sesame yield.
{"title":"Growth and Yield Response of Black Sesame (Sesamum indicum L.) to Foliar Spray of Amendments as Plant Growth Regulators and Micronutrients in Field Conditions","authors":"Tuan Ntq, Hue Nh, Khuong Nq, Huu Tn, Thuc Lv","doi":"10.26420/annagriccropsci.2021.1083","DOIUrl":"https://doi.org/10.26420/annagriccropsci.2021.1083","url":null,"abstract":"To improve the yield of sesame in Mekong delta of Vietnam, the study was conducted to find out plant growth regulator, micronutrient to increase growth and yield of black sesame. The experiment consisted of six treatments (NT) as follows: NT1: Control as no plant growth regulators and micronutrients, NT2: Spraying brassinolide at 50ppm, NT3: Spraying gibberellin at 100ppm and manganese at 0.05%, NT4: Spraying brassinolide at 1.25ppm, NT5: Spraying boric at 50ppm and molybdenum at 50ppm, NT6: Spraying selenium at 4ppm. The results showed that spraying plant growth regulators and micronutrients as single NAA, BR, Se and combined GA3 plus manganese, boric plus molybdenum at 27 and 35 days after sowing have not increased plant height and height to the first fruit in the first crop. Combination of spraying boric plus molybdenum or single selenium increased the number of capsules per plant, the number of seeds per capsule and resulted in higher sesame yield.","PeriodicalId":8133,"journal":{"name":"Annals of Agricultural & Crop Sciences","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90361849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-14DOI: 10.26420/annagriccropsci.2021.1082
M. G., B. O.
Beans are part of the basic diet alimentation for Brazilian population, as they gather proteins, carbohydrates, vitamins, mineral salts, fibers, amino acids and essential nutrients such as iron and calcium, being a complete food that can be compared with the amount of protein that the meat has. Considering the beans world production, in development countries represent almost 50%, being that Myanmar, India and Brazil the top three position. The use of pesticides is widely spread in these countries to reduce agricultural losses due to pests that interfere with grain production. Therefore the risk that could be generated from foods pesticides residues makes their analyses of quantification mandatory. The purpose of this work was to develop an analytical method to quantitatively characterize fungicides pesticides residues, flutriafol, procymidone and tebuconazole that were used to angular spot control, anthracnose, rust and alternaria spot, white mold fungi, present in beans, by means of gas chromatography coupled with a triple quadrupole mass spectrometer. Samples of beans, Phaseolus vulgaris L, types white, black, string and Vigna angularis, type adzuki, had been bought in grains store and supermarkets at metropolitan São Paulo city. The validation of analytical method was explored for sensitivity, selectivity, precision. The extraction procedure was performed in two different forms, QuEChERS, and solid-liquid extraction with low temperature. Through this methodology, reached below the maximum limit allowed by Brazilian law 0.5mgkg-1 for procymidone and 0.1mgkg-1 for flutriafol and tebuconazole. Several samples of four types of beans were tested and all of them had procymidone identified and 7% of samples higher than the law limit.
{"title":"Pesticides Analysis in Beans by Gas Chromatography Couplet with Tandem Mass Spectrometry","authors":"M. G., B. O.","doi":"10.26420/annagriccropsci.2021.1082","DOIUrl":"https://doi.org/10.26420/annagriccropsci.2021.1082","url":null,"abstract":"Beans are part of the basic diet alimentation for Brazilian population, as they gather proteins, carbohydrates, vitamins, mineral salts, fibers, amino acids and essential nutrients such as iron and calcium, being a complete food that can be compared with the amount of protein that the meat has. Considering the beans world production, in development countries represent almost 50%, being that Myanmar, India and Brazil the top three position. The use of pesticides is widely spread in these countries to reduce agricultural losses due to pests that interfere with grain production. Therefore the risk that could be generated from foods pesticides residues makes their analyses of quantification mandatory. The purpose of this work was to develop an analytical method to quantitatively characterize fungicides pesticides residues, flutriafol, procymidone and tebuconazole that were used to angular spot control, anthracnose, rust and alternaria spot, white mold fungi, present in beans, by means of gas chromatography coupled with a triple quadrupole mass spectrometer. Samples of beans, Phaseolus vulgaris L, types white, black, string and Vigna angularis, type adzuki, had been bought in grains store and supermarkets at metropolitan São Paulo city. The validation of analytical method was explored for sensitivity, selectivity, precision. The extraction procedure was performed in two different forms, QuEChERS, and solid-liquid extraction with low temperature. Through this methodology, reached below the maximum limit allowed by Brazilian law 0.5mgkg-1 for procymidone and 0.1mgkg-1 for flutriafol and tebuconazole. Several samples of four types of beans were tested and all of them had procymidone identified and 7% of samples higher than the law limit.","PeriodicalId":8133,"journal":{"name":"Annals of Agricultural & Crop Sciences","volume":"60 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87888128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-05-18DOI: 10.26420/annagriccropsci.2021.1081
Raja Ia
To achieve energy security and to address energy related environmental issues attempts have been made to find out such energy resources that are economically viable and environmentally friendly. Biogas appears as a sustainable, renewable and carbon neutral energy source, a substitute to reduce the global fossil fuels dependency. Agricultural activities generate huge amounts of organic residues annually worldwide. Microbial conversion of agriculture residue and organic wastes to produce biogas offers an attractive way for energy supply, resource recovery and waste treatment. Energy generated is renewable can have positive impact on environment, replacing fossil fuels and mitigating greenhouse gases emissions. In addition to a clean and cost effective energy source, it improves the management of manure and organic wastes and replaces inorganic fertilizer. Biogas production in the agricultural sector is a fast growing market particularly in many European countries. This article is aimed to review and investigate the potential contribution of biogas from agricultural residues. Techniques for quantitative assessment of the residue from different crops that can be recovered sustainably as a potential resource for biogas production are discussed.
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Pub Date : 2021-05-13DOI: 10.26420/annagriccropsci.2021.1080
X. Chao-qun, H. Li-wei, G. Jian-hua, S. SongJi-Zhen, M. Wen-Jun, X. Jia-qin, Z. Shi-xiang, X. Xian-yi
80 samples of flue-cured tobacco leaves in Ganzhou area, Jiangxi Province in China were selected, relationships between free amino acid index and burntmellow- sweet aroma style intensity of leaves were studied with correlation analysis and stepwise regression analysis. In contrast to the content of Trp, Ile, Ser, Cys, β-Ala, Lys and the ratio of β-AiBA/TFAA, the content of Asp, Ala, Gly, Leu, β-AiBA and the ratio of Trp/TFAA, Ile/TFAA, Val/TFAA, Thr/TFAA, Ser/TFAA, Cys/TFAA, Asn/TFAA, β-Ala/TFAA, Lys/TFAA and Pro/TFAA in fluecured tobacco leaves had extremely significant positive correlation with burntmellow- sweet aroma style intensity. There was a significant negative correlation between burnt-mellow-sweet aroma style intensity and the content of Phe, Tyr, Asn, γ-ABA. 31 free amino acid indexes including the content of Asp, Glu, Phe etc. and the ratio of Glu/TFAA, Phe/TFAA, Trp/TFAA, etc. were main free amino acid index influencing burnt-mellow-sweet aroma style intensity of flue-cured tobacco leaves, which jointly decides 99.1% of variation of burnt-mellow-sweet aroma style intensity.
{"title":"Relationship between Free Amino Acid Index and Burnt-Mellow-Sweet Aroma Style Intensity of Flue-Cured Tobacco Leaves","authors":"X. Chao-qun, H. Li-wei, G. Jian-hua, S. SongJi-Zhen, M. Wen-Jun, X. Jia-qin, Z. Shi-xiang, X. Xian-yi","doi":"10.26420/annagriccropsci.2021.1080","DOIUrl":"https://doi.org/10.26420/annagriccropsci.2021.1080","url":null,"abstract":"80 samples of flue-cured tobacco leaves in Ganzhou area, Jiangxi Province in China were selected, relationships between free amino acid index and burntmellow- sweet aroma style intensity of leaves were studied with correlation analysis and stepwise regression analysis. In contrast to the content of Trp, Ile, Ser, Cys, β-Ala, Lys and the ratio of β-AiBA/TFAA, the content of Asp, Ala, Gly, Leu, β-AiBA and the ratio of Trp/TFAA, Ile/TFAA, Val/TFAA, Thr/TFAA, Ser/TFAA, Cys/TFAA, Asn/TFAA, β-Ala/TFAA, Lys/TFAA and Pro/TFAA in fluecured tobacco leaves had extremely significant positive correlation with burntmellow- sweet aroma style intensity. There was a significant negative correlation between burnt-mellow-sweet aroma style intensity and the content of Phe, Tyr, Asn, γ-ABA. 31 free amino acid indexes including the content of Asp, Glu, Phe etc. and the ratio of Glu/TFAA, Phe/TFAA, Trp/TFAA, etc. were main free amino acid index influencing burnt-mellow-sweet aroma style intensity of flue-cured tobacco leaves, which jointly decides 99.1% of variation of burnt-mellow-sweet aroma style intensity.","PeriodicalId":8133,"journal":{"name":"Annals of Agricultural & Crop Sciences","volume":"122 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73912537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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