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Infectious Bronchitis Disease in Poultry its Diagnosis, Prevention and Control Strategies 家禽传染性支气管炎的诊断与防治策略
Pub Date : 2021-10-20 DOI: 10.26420/annagriccropsci.2021.1100
Samad A, A. A, Mehtab U, Ur Rehman Ali Khera H, Rehman A, H. M
Avian Infectious bronchitis (IB) is profoundly one of the main diseases associated with respiratory syndrome in domestic poultry causing significant economic losses to poultry production. Avian Infectious Bronchitis (IB) has a place with Coronaviridae family, which causes respiratory illness, diminished creation of flying creatures, nephrotic Syndrome, and unsalvageable harm to oviduct, which can cause the crenation of anomalous eggs. Inoculation programs currently are pointless because of the outrageous hereditary varieties of IBV. Consequently, an exact and fast serotype assurance is a significant factor in controlling IBV. Compelling analytic instruments are likewise expected to analyze IB diseases in the field and to distinguish diverse serotypes and variations. Ideal administration for forestalling IB in flying creatures incorporates ideal inoculation with live or inactivated Vaccines containing flowing strains and severe disconnection of the contaminated fowls. Great administration and sterile practices in poultry units can handle the spread of IB among groups by and large. The idea of DNA vaccination with spike protein quality has altered the idea of IB immune prophylaxis as it has been found to evoke an adequate invulnerable reaction. Atomic science-based identification and control procedures must be created alongside the up degree of customary strategies to handle the developing danger presented by this microorganism the sickness can be adequately controlled in the years.
禽传染性支气管炎是与家禽呼吸综合征相关的主要疾病之一,对家禽生产造成重大经济损失。禽传染性支气管炎(IB)与冠状病毒科同属一类,可引起呼吸系统疾病、飞行生物繁殖减少、肾病综合征和对输卵管造成不可挽回的伤害,从而导致异常卵的产生。接种计划目前是毫无意义的,因为IBV的遗传变种令人发指。因此,准确、快速地确定血清型是控制IBV的重要因素。令人信服的分析仪器也有望在现场分析IB疾病,并区分不同的血清型和变异。在飞行生物中预防IB的理想管理包括理想的接种含有流动毒株的活疫苗或灭活疫苗和污染家禽的严重分离。家禽单位的严格管理和无菌做法可以处理IB在群体中的传播。具有刺突蛋白质量的DNA疫苗接种的想法已经改变了IB免疫预防的想法,因为已经发现它可以引起足够的无懈可击的反应。必须建立基于原子科学的鉴定和控制程序,以及提高习惯策略的程度,以处理这种微生物所带来的发展危险,多年来这种疾病可以得到充分控制。
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引用次数: 5
Manganese Hyperaccumulators and their Hyperaccumulating and Tolerance Mechanisms: A Review of the Current State of Knowledge 锰超蓄积体及其超蓄积和耐受机制:目前的研究进展
Pub Date : 2021-10-19 DOI: 10.26420/annagriccropsci.2021.1099
Pang W.S., Wu K-K, Rajendran M, L. W.C., W. C.
Manganese (Mn) is ubiquitous in the environment due to both geological and human activities. It is essential for plants, as for most other living organisms, but can also be toxic when it is present in excess. Some plant species, referred to as Mn hyperaccumulators, can accumulate over 10000μg/g of Mn in their shoot tissues without showing any phytotoxicity. Approximately 24 Mn hyperaccumulators are currently known worldwide. However, ample data is available the Mn hyperaccumulator species and biological significance of Mn hyperaccumulation and tolerance mechanisms. To give new insights, this review highlights the current knowledge of Mn hyperaccumulation and tolerance mechanisms in hyperaccumulators, which include root uptake, xylem loading, transport, sequestration, and detoxification processes. Hyperaccumulators uptake Mn mainly accumulates as Mn2+ into the xylem, from which it is then transferred to the shoots. Foliar Mn2+ is mainly stored in vacuoles, the endoplasmic reticulum, and the Golgi. It is sequestered by organic ligands and some transporter proteins at a subcellular level in the root and shoot, which can allow the plants to exhibit great tolerance. From the in-depth examine the published literature; the main knowledge gap and future research are highlighted. In addition, Mn hyperaccumulator biomass disposal methods and applications also discussed.
由于地质和人类活动的原因,锰在环境中普遍存在。它对植物和大多数其他生物都是必不可少的,但当它过量存在时也可能是有毒的。一些被称为锰超积累者的植物可以在其茎部组织中积累超过10,000 μg/g的锰而不表现出任何植物毒性。目前世界上已知的Mn超蓄积物大约有24种。然而,锰超积累体的种类、生物学意义和耐受机制等方面的研究资料比较丰富。为了提供新的见解,本文综述了目前对锰的超积累和耐受机制的了解,包括根吸收、木质部装载、运输、固存和解毒过程。超积累体吸收锰主要以Mn2+的形式积累到木质部,然后从木质部转移到茎部。叶片Mn2+主要储存在液泡、内质网和高尔基体中。它被有机配体和一些转运蛋白在亚细胞水平上隔离在根和茎中,这可以使植物表现出很强的耐受性。从深入考察已发表文献入手;强调了主要的知识缺口和未来的研究方向。此外,还讨论了Mn超蓄能器的生物质处理方法及应用。
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引用次数: 0
Regional Students’ Vulnerability to Climate in Tunisia: A Spatial Analysis 突尼斯地区学生气候脆弱性的空间分析
Pub Date : 2021-10-15 DOI: 10.26420/annagriccropsci.2021.1098
Zouabi O, Bouhlila Ds
Weather conditions have not been considered up to date as a factor that contributes to drop out of school in Tunisia. The latter is mainly attributed to poverty, socioeconomic status of students and to a lack of educational infrastructure. This paper is an attempt to introduce geography in explaining dropout in Tunisia. We apply spatial econometric models to show that there is a high degree of spatial clustering in dropout of school. We conclude that students in the north-west region of Tunisia are those who are the most vulnerable to weather conditions and to climate change.
在突尼斯,天气状况迄今尚未被认为是导致辍学的一个因素。后者主要归因于贫困、学生的社会经济地位和缺乏教育基础设施。本文试图引入地理学来解释突尼斯的辍学现象。运用空间计量模型分析了我国辍学现象的空间聚类程度。我们得出的结论是,突尼斯西北地区的学生最容易受到天气条件和气候变化的影响。
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引用次数: 0
Molecular Characterization and Population Structure Analysis of Pepper Mild Mottle Virus Infecting Capsicum in Himachal Pradesh, India 印度喜马偕尔邦辣椒轻度斑驳病毒分子特征及群体结构分析
Pub Date : 2021-10-13 DOI: 10.26420/annagriccropsci.2021.1097
K. N, Patel Pb, Chaudhary S, Sharma Pn
Pepper Mild Mottle Virus (PMMoV) is posing a great threat to capsicum cultivation worldwide. Based on the ability of PMMoV in overcoming L mediated resistance in Capsicum spp., it has been categorized in to five pathotypes. The information of pathotype is crucial in framing sustainable management practices. The surveys conducted in seven districts of Himachal Pradesh (H.P) during 2016 and 2017 revealed maximum incidence of PMMoV in Kullu (88.89%) district followed by Mandi (78.57%) after confirmation through DAS-ELISA and RT-PCR. A total 16 isolates were selected for their pathotype characterization by deducing their Amino Acid (aa) sequence and their pathogenicity on differential capsicum cultivars carrying L1, L2, L+, L3 and L4 genes. None of the test isolates produced visible symptoms on differential cultivarsPI-159236 and PI-260429 carrying L3 and L4 genes, respectively. The aa sequences of all test isolates except PMMoV-16.6, 16.10 and PMMoV-17.1 were 100% similar to the P12 pathotypes available at NCBI database. Additionally, the 3-D structure of PMMoV-16.6, 16.10 and 17.1 isolates constructed using SWISSMODEL affirmed the presence of mutations in the short and long hairpin loops of viral CP corresponding to the inner surface and thus do not contribute to the elicitor activity of CP. Thus, all the present isolates were characterized as P12 pathotype. The full genome sequences of three isolates viz., PMMoV-16.7 (MN496153), 16.9 (MN496154) and 17.3 (MN734123) determined in the present study revealed their maximum similarity with PMMoV-HP1 (P12, KJ631123.1) and a Japanese isolate (P12, AB000709.2) and least with a Korean isolate (LC082100.1). The full genome-based recombination analysis did not show any significant recombination event.
辣椒轻度斑驳病毒(PMMoV)是全球辣椒种植的一大威胁。根据PMMoV在辣椒中克服L介导抗性的能力,将其分为5种致病型。病型信息对于构建可持续管理实践至关重要。2016年和2017年在喜马偕尔邦(hp) 7个县进行的调查显示,经DAS-ELISA和RT-PCR证实,PMMoV发病率最高的是Kullu县(88.89%),其次是Mandi县(78.57%)。通过氨基酸(aa)序列和对携带L1、L2、L+、L3和L4基因的不同辣椒品种的致病性分析,筛选出16株菌株进行病原型鉴定。在分别携带L3和L4基因的差异栽培株spi -159236和PI-260429上,试验分离株均未产生明显症状。除PMMoV-16.6、16.10和PMMoV-17.1外,所有分离株的aa序列与NCBI数据库中P12型的相似度均为100%。此外,利用SWISSMODEL构建的PMMoV-16.6、16.10和17.1分离株的三维结构证实了病毒CP内表面对应的短发夹环和长发夹环存在突变,因此与CP的激发子活性无关,因此所有分离株均为P12病型。PMMoV-16.7 (MN496153)、16.9 (MN496154)和17.3 (MN734123)的全基因组序列与PMMoV-HP1 (P12, KJ631123.1)和日本分离株(P12, AB000709.2)的相似性最大,与韩国分离株(LC082100.1)的相似性最小。基于全基因组的重组分析未显示任何显著的重组事件。
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引用次数: 1
Evaluation of Chickpea Varieties Growing on Residual Moisture of Vertisols in Southern Ethiopia 埃塞俄比亚南部鹰嘴豆品种在土壤残湿条件下生长的评价
Pub Date : 2021-10-06 DOI: 10.26420/annagriccropsci.2021.1096
Allito Bb, Geda Cg
Vertisols are suited to dry-land crop production in semi-arid environments since the residual moisture in the soil can support crop growth after the end of the rainy season. However, traditional cropping systems seldom utilize residual moisture of Vertisols for crop production. Farmers need suitable crop species to sustain and enhance their production under such soil and climatic conditions. Chickpea is the strategic crop well adapted to Vertisols and derives most of its water requirements from the residual soil moisture. Yet, different chickpea varieties found to adapt and produce different yields at different locations. The experiments were conducted for two consecutive years (2016 and 2017) on farmers training centers in two districts of Southern Ethiopia. Six released varieties; Arerti, Habru, Yelibe, Naatolii, Fetenech, Kutaye and a Local check were used for the experiment. The trials were laid in a randomized complete block design with four replicates. Data were collected on phenological traits, growth and yield attributes, and grain yield. The results showed that there was significant variation among the tested chickpea varieties in all the agronomic traits recorded. Varieties Arerti and Habru revealed superiority in grain yield, pod setting, hundred seed weight and biomass over the Local check across locations. Based on yielding performance and farmers’ preference, varieties Arerti and Habru are recommended for production in the selected districts and similar agro-ecologies in southern Ethiopia.
由于土壤中的剩余水分在雨季结束后可以支持作物生长,因此垂直土壤适合半干旱环境中的旱地作物生产。然而,传统的种植系统很少利用versols的残余水分进行作物生产。在这样的土壤和气候条件下,农民需要合适的作物品种来维持和提高产量。鹰嘴豆是一种战略作物,很好地适应了干旱,它的大部分水分需求来自土壤残留水分。然而,不同的鹰嘴豆品种在不同的地方适应并产生不同的产量。实验连续两年(2016年和2017年)在埃塞俄比亚南部两个地区的农民培训中心进行。6个已发布品种;实验中使用了areti, Habru, Yelibe, Naatolii, Fetenech, Kutaye和Local check。试验采用随机完全区组设计,共设4个重复。收集了物候性状、生长和产量性状以及籽粒产量的数据。结果表明,鹰嘴豆品种间农艺性状均存在显著差异。品种Arerti和Habru在籽粒产量、结荚率、百粒重和生物量方面均优于Local check。根据产量表现和农民的偏好,推荐在选定的地区和埃塞俄比亚南部类似的农业生态中生产Arerti和Habru品种。
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引用次数: 0
Effect of Phytase Transgenic Corn Supplemented in Feed Diets on the Utilization of Calcium and Phosphorus in Bama Boars 饲粮中添加植酸酶转基因玉米对巴马公猪钙磷利用的影响
Pub Date : 2021-10-05 DOI: 10.26420/annagriccropsci.2021.1095
Liqin Lh, J. W., D. M., Min Li, Xiaoguang Y, L. Y.
The study aimed to evaluate the nutritional composition and the efficacy of phytase transgenic corn. Ten-month old castrated Bama boars and replicated a 6×6 Latin square design were used to evaluate the effect of phytase on utilization of calcium of phosphorus. Six-dietary treatments included: Phosphorus adequate control diet (PC), PTC0 (low-phosphorus diet), PTC500 (lowphosphorus diet with 500 FTU phytase/kg in PTC), PTC1000 (low-phosphorus diet with 1000 FTU phytase/kg in PTC), PTC5000 (low-phosphorus diet with 5000 FTU phytase/kg in PTC), microbe500 (low-phosphorus diet with 500 FTU phytase/kg from Aspergillus Niger). Contents of the proximate components, minerals, vitamins and amino acid of PTC did not differ from CC. The PTC has lower concentrations of phytate phosphorus than CC, the apparent digestibility of calcium and phosphorus of the PTC0 group is lower (p<0.05). No obvious dose-dependent relationship about the apparent digestibility of calcium and phosphorus showed among PTC500, PTC1000 and PTC5000. Therefore, the nutritional compositions of PTC had substantial equivalence to that of control corn except for phytate phosphorus concentrations, the PTC addition can improve the apparent digestibility of phosphorus and calcium and reduce the excrement of inorganic phosphorus, PTC might be an economically efficient way to decrease environment pollutions.
本研究旨在评价植酸酶转基因玉米的营养成分和功效。采用6×6拉丁方设计,研究植酸酶对10月龄阉割巴马公猪钙磷利用的影响。6种饲粮处理分别为:磷足量对照饲粮(PC)、PTC0(低磷饲粮)、PTC500(低磷饲粮,PTC中植酸酶含量为500 FTU /kg)、PTC1000(低磷饲粮,PTC中植酸酶含量为1000 FTU /kg)、PTC5000(低磷饲粮,PTC中植酸酶含量为5000 FTU /kg)、microbe500(低磷饲粮,PTC中植酸酶含量为500 FTU /kg)。PTC的近组分、矿物质、维生素和氨基酸含量与CC无显著差异,PTC的植酸磷浓度低于CC, PTC0组钙、磷的表观消化率低于CC (p<0.05)。PTC500、PTC1000和PTC5000对钙、磷的表观消化率没有明显的剂量依赖关系。因此,除植酸磷浓度外,PTC的营养成分与对照玉米基本相当,添加PTC可以提高磷和钙的表观消化率,减少无机磷的排泄量,PTC可能是一种经济有效的减少环境污染的方法。
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引用次数: 0
Stress Effect Induced by Microplastics Coupled with Acid Rain, on Garden Cress, During Short and Long Time: Two Exposures in Comparison 微塑料与酸雨对园菜短时间和长时间胁迫效应的比较
Pub Date : 2021-09-09 DOI: 10.26420/annagriccropsci.2021.1094
Pignattelli S, B. A., R. M
Aim: Plastics, and acid rain, are the results of the anthropogenic activities; although, the injuries caused by acidic precipitation to plants are well known, what happen if acid rain is coupled with microplastics pollution? Methods: In this experiment, we simulated, under controlled condition, a contaminated area from four different kinds of Microplastics (MPs): Polyethylene (PE), Polyvinylchloride (PVC), commercial mixture (PE + PVC) and Polypropylene (PP) subjected to acidic precipitation on Lepidium sativum, both in short then long exposures. At the end of experimentation were analysed: i) plant biometrical parameters (percentage inhibition of seed germination, plant height, leaf number and fresh biomass productions); and ii) oxidative stress (e.g., levels of hydrogen peroxide, glutathione, and ascorbic acid). On plant subjected to long exposure are quantified also: lipidic peroxidation through Malondialdehyde (MDA) concentration, Aminolevulinic Acid (AlA), pigments (Chl-a, Chl-b, carotenoids) and soluble sugars. Results: Our results suggest that root system is more sensitive to MPs coupled with acid rain then above ground biomass. Furthermore, in short exposure toxicity begin with PE+ treatments to shift towards PVC+ toxicity in long exposure at radical level. Conclusion: Our experiment demonstrates that different kinds of microplastics supplied with acid rain are able to interfere in different ways both in short and in long exposure, but also at shoot and root levels, on garden cress.
目的:塑料和酸雨是人类活动的结果;虽然,酸雨对植物造成的伤害是众所周知的,但如果酸雨加上微塑料污染会发生什么呢?方法:在控制条件下,模拟了四种不同微塑料(MPs):聚乙烯(PE)、聚氯乙烯(PVC)、商业混合物(PE + PVC)和聚丙烯(PP)在Lepidium sativum上的短时和长时间酸性沉淀污染区域。实验结束时分析:i)植物生物学参数(抑制种子萌发率、株高、叶数和新鲜生物量);ii)氧化应激(如过氧化氢、谷胱甘肽和抗坏血酸的水平)。对长期暴露的植物也进行了定量:通过丙二醛(MDA)浓度、氨基乙酰丙酸(AlA)、色素(Chl-a、Chl-b、类胡萝卜素)和可溶性糖进行的脂质过氧化。结果:与地上生物量相比,根系对MPs和酸雨的耦合更为敏感。此外,在短期暴露中,毒性从PE+处理开始,在长期暴露中在自由基水平上转向PVC+毒性。结论:我们的实验表明,酸雨中不同种类的微塑料在短期和长期暴露中,以及在茎和根水平上,都能以不同的方式干扰园艺作物。
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引用次数: 1
Efficacy of Different Fungicides Against Rhizome Rot Disease of Ginger Under Farmer Field Condition in Salyan, Nepal 不同杀菌剂在尼泊尔萨利安农民田间条件下对生姜根茎腐病的防治效果
Pub Date : 2021-09-04 DOI: 10.26420/annagriccropsci.2021.1093
R. B, A. P, A. P., B. G., Babu Bk
A study was conducted from March to August 2020 to evaluate the effectiveness of different fungicides for the management of rhizome rot disease in ginger. The field experiment was conducted under Randomized Complete Block Design with four replication and five treatments; Carbendazim 50% WP @ 1gm/litre + Mancozeb 75% WP @ 2.5gm/litre, Metalaxyl 8% WP + Mancozeb 64% WP @ 2gm/litre, Carbendazim 50% WP @ 1gm/litre, Trichoderma viride @ 5gm/litre and control. The height of pseudo stems, leaf number, germination percentage, tiller number, disease incidence, disease severity and percentage of disease control was recorded during field experiment. There was no significant effect of pre sowing rhizome treatment in height of pseudostems, leaf number and germination percentage. The highest tiller number (3.40) was found with Carbendazim 50% WP @1gm/litre + Mancozeb 75% @ 2.5gm/ litre treatment and lowest tillers number (2.95) was found with control. The highest disease incidence (34.37) and severity (30.03) was found in control plot whereas the lowest disease incidence (2.58) and disease severity (2.46) was found with Carbendazim 50% @ 1gm/litre treatment + Mancozeb 75% @ 2.5gm/litre. Similarly, the highest percentage disease control (85.61) was found with Carbendazim 50% @ 1gm/litre + Mancozeb 75% @ 2.5gm/litre treated rhizome and the lowest (54.14) was found with Trichoderma viride @ 5gm/litre treatment. Therefore, seed treatment with Carbendazim 50%WP @ 1gm/litre + Mancozeb 75% WP @ 2.5 gm/litre is found effective among other fungicides in Bagchaur-3, Salyan and is recommended for enhancing tiller and reducing rhizome rot disease incidence and severity in ginger.
2020年3月至8月,研究了不同杀菌剂对生姜根茎腐病的防治效果。田间试验采用随机完全区组设计,4个重复,5个处理;多菌灵50% WP @ 1gm/升+代森锰锌75% WP @ 2.5gm/升,甲氨酯8% WP +代森锰锌64% WP @ 2gm/升,多菌灵50% WP @ 1gm/升,绿木霉@ 5gm/升和对照。田间试验时记录假茎高、叶片数、发芽率、分蘖数、发病率、病情严重程度和防病率。播前根茎处理对假茎高、叶片数和发芽率无显著影响。多菌灵50% WP @1gm/l +锰锌75% @ 2.5gm/ l处理的分蘖数最高(3.40),对照的分蘖数最低(2.95)。对照区发病率最高(34.37),严重程度最高(30.03),多菌灵50% @ 1gm/l +代森锰锌75% @ 2.5gm/l组发病率最低(2.58),严重程度最低(2.46)。同样,多菌灵50% @ 1gm/升+代森锰锌75% @ 2.5gm/升处理根茎的防病率最高(85.61),绿木霉@ 5gm/升处理根茎的防病率最低(54.14)。因此,与其他杀菌剂相比,多菌肼50%WP @ 1gm/升+代森锰锌75% WP @ 2.5 gm/升的种子处理在Bagchaur-3和Salyan中是有效的,并被推荐用于提高分蘖率和降低生姜根茎腐病的发病率和严重程度。
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引用次数: 0
Changes in Hepatic Metabolism of Rattus norvegicus Infected to Angiostrongylus cantonensis (Nematoda) and Exposed to Glyphosate-Based Herbicide 褐家鼠感染广东管圆线虫和草甘膦除草剂后肝脏代谢的变化
Pub Date : 2021-09-02 DOI: 10.26420/annagriccropsci.2021.1092
Braga Bv, Garcia Js, Simões Ro, Silva Jsp, Castro Ls, S. Ch, Maldonado Júnior A
Helminth infection associated with exposure to pesticides has received little attention regarding its effect on the human population and on farm and wild animals. The aim of this study was to evaluate the effects a glyphosate-based herbicide on the hepatic and glycemic metabolism of Rattus norvegicus (Wistar) infected by Angiostrongylus cantonensis. Experimental groups were orally infected with 50 L3 larvae of A. cantonensis and exposed to the herbicide after and before the infection. Biochemical serum analyses were carried out to determine the levels of Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT), Alkaline Phosphatase (ALP), Total Bilirubin (TB), total protein, albumin, urea, creatinine, uric acid, glucose and hepatic glycogen. All exposed groups showed an increase in the concentration of glycogen, AST, ALT and TB, the last ones suggesting liver tissue damage. Exposure to the herbicide caused hyperalbuminemia as an antioxidant response to the herbicide. These findings contribute to a better understanding of how glyphosate-based herbicides can change the hepatic metabolism the vertebrate and to influence the parasite-host relationship.
与接触农药有关的蠕虫感染对人类以及农场和野生动物的影响很少受到关注。本研究旨在评价草甘膦除草剂对广东管圆线虫感染褐家鼠肝脏代谢和血糖代谢的影响。试验组分别经口感染50只广州蠓L3幼虫,感染前后分别暴露于除草剂中。进行血清生化分析,测定血清中天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)、碱性磷酸酶(ALP)、总胆红素(TB)、总蛋白、白蛋白、尿素、肌酐、尿酸、葡萄糖和肝糖原的水平。各暴露组肝糖原、AST、ALT和TB浓度均升高,后者提示肝组织损伤。暴露于除草剂引起高白蛋白血症,作为对除草剂的抗氧化反应。这些发现有助于更好地理解草甘膦除草剂如何改变脊椎动物的肝脏代谢并影响寄生虫-宿主关系。
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引用次数: 0
Agricultural Bio-Stimulant Activity of Fish Protein Hydrolysate from Common Silver Belly (Gerres subfasciatus) 银腹鱼蛋白水解物的农业生物刺激素活性
Pub Date : 2021-09-02 DOI: 10.26420/annagriccropsci.2021.1091
S. S., D. R., S. M
Taking into account of using the fish industry wastes and for expanding the incentive to a few under used fish species, protein hydrolysates from fish proteins are being set up by a few specialists everywhere throughout the world. Fish Protein Hydrolysates (FPH) are breakdown results of enzymatic transformation of fish proteins into smaller units like peptides, which typically contain 2-20 amino acids. Their worth has been expanded by utilizing them as fluid bio compost, feed supplement and bioorganic fertilizer. From the research carried out at our laboratory, Dry fish silver belly with both microbe and enzyme acquired from cultural broth of lactobacillus species, catalyst papain demonstrated positive upgrade of development and advancement of plants tested. In view of the yield of FPH concentrates from both microbial and enzymatic hydrolysis, the pace of seedling life list in Urad dhal, green gram and okra got higher in 0.1-0.5% convergence of dry fish hydrolysate from lactobacillus treated than in the control variation. With respect to the seeds of urad dhal, green gram and okra, the germination rate and seedling energy list from treated seeds surpassed control by 19.11-128.71%. The positive effect of dry fish protein hydrolysate by microbial procedure on germination rate, seedling life record rate in 3 kinds of seeds is the reason for the improvement of protein hydrolysate item for application in crop creation utilizing dry fish squanders. The Seedling grown in 0.5% concentration of fish hydrolysates showed significant increase in seedling vigour index of Urad dhal (13.29 ± 0.12 %), Green gram (14.1 ± 0.17%) and Okra (5.39 ± 0.43 %) was recorded through one way Analysis of Variance (ANOVA). By HPLC determination amino acid results determined that protein hydrolysates process can be stopped on 7th day (643.56ppm) whereas @ 10th day amino acid decreased (326.24ppm). Results suggest that probiotic assumes a superior job in dry fish protein hydrolysates than compounds when contrasted and yield and prudent astute.
考虑到利用渔业废料和扩大对少数未充分利用的鱼类的奖励,世界各地的一些专家正在从鱼类蛋白质中提取蛋白质水解物。鱼蛋白水解物(FPH)是酶将鱼蛋白转化为更小的单位(如肽)的分解结果,通常含有2-20个氨基酸。利用它们作为液体生物堆肥、饲料添加剂和生物有机肥,扩大了它们的价值。从我们实验室开展的研究中,从乳酸菌种的培养肉汤中获得微生物和酶,催化剂木瓜蛋白酶,干鱼银肚显示了被测植物的积极发展和进步。从微生物酶解和酶解的FPH浓缩液产量来看,乳杆菌发酵的鱼干水解液在0.1-0.5%的收敛量下,乌拉尔、绿克和秋葵的幼苗寿命表速度高于对照变化。乌拉尔、绿克和秋葵种子的发芽率和幼苗能表比对照高19.11% ~ 128.71%。经微生物处理的鱼干蛋白水解物对3种种子的发芽率和幼苗寿命记录率的积极影响,是改进鱼干废弃物作物生产中应用的蛋白水解物项目的原因。经单因素方差分析(ANOVA),在0.5%浓度的鱼解液中培养的幼苗活力指数显著提高了乌拉尔(13.29±0.12%)、绿克(14.1±0.17%)和秋葵(5.39±0.43%)。高效液相色谱法测定氨基酸结果表明,蛋白水解过程在第7天可以停止(643.56ppm),而在第10天氨基酸含量下降(326.24ppm)。结果表明,在鱼干蛋白水解物中,益生菌的作用优于化合物。
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Annals of Agricultural & Crop Sciences
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