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Colchicine Induced Polyploidy in Cassia tora (L.) Rox. 秋水仙碱诱导决明子多倍体的研究火箭。
Pub Date : 2020-03-14 DOI: 10.5281/APS.2020.9.3.4
Bihari Singh, R. Kumar, A. K. Nag, Anil Kumar Singh, P. Chandra, V. Patel
An Induction of artificial polyploidy by 0.4% aqueous solution of Colchicine has been successfully made in Cassia tora . The data obtained from the experimentally treated materials have been further analysed for all the implications to understand the effect of Colchicine on the Chromosome structure, and function. The tetraploids showed n = 26 bivalents and different combinations of univalent and multivalent, the percentage of quadrivalents being the highest Chromosome irregularities in PMCs of polyploids has been detected more frequently than normal diploid. The polyploids thus, raised did show autopolyploidy in nature. The chemical environment within the cell can change the meiotic behaviour of a species. The tetraploid plants showed a slower rate of growth, delayed flowering, thicker, darker and wider leaflet, and decreased stomatal frequency per unit area. This also showed larger stomata, increase in height, increase in number of lateral branching, bigger fruit, seed size and increase in the number of pollen grains with absence of exine. The pollen sterility increases as compared to diploid. It is thus clear that experimental induction by Chemical means (Colchicine) holds a clue for understanding the adaptability of the species in different environmental conditions.
用0.4%秋水仙碱水溶液成功诱导决明子的人工多倍体。从实验处理的材料中获得的数据已被进一步分析,以了解秋水仙碱对染色体结构和功能的影响。四倍体有26个二价体和不同的一价和多价组合,四价体是多倍体PMCs中染色体不规则率最高的染色体,比正常二倍体更常见。因此,培养的多倍体在自然界中确实表现出自多倍体。细胞内的化学环境可以改变物种的减数分裂行为。四倍体植株生长速度较慢,开花时间较晚,小叶较厚、颜色较深、较宽,单位面积气孔频率降低。气孔增大,株高增高,侧分枝数增多,果实、种子大小增大,花粉粒数增多,无外皮。与二倍体相比,花粉不育性增加。因此,通过化学方法(秋水仙碱)的实验诱导为了解该物种在不同环境条件下的适应性提供了线索。
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引用次数: 0
Pseudephemerum (Limb.) I. Hagen (Dicranaceae, Dicranales, Bryopsida) A new generic record to Peninsular India. Pseudephemerum(肢)。1 . Hagen (Dicranaceae, dicrananales, Bryopsida)印度半岛新属记录。
Pub Date : 2020-03-14 DOI: 10.5281/APS.2020.9.3.3
Ravi Prasad Rao Boyina, Sreenath Ananthaneni
Pseudephemerum (Limb.) I. Hagen , represented by P. nitidum (Hedw.) Loeske, collected from the forests of Banda Village, Visakhapatnam district, Andhra Pradesh is a new generic record to Peninsular India.
Pseudephemerum(肢)。I. Hagen,以P. nitidum (hew)为代表。从安得拉邦维萨卡帕特南地区班达村的森林中收集的Loeske是印度半岛的一种新的一般记录。
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引用次数: 1
Effect of vermicompost on Andrographis paniculata L. grown in stress condition of Chlorpyrifos 虫粪对毒死蜱胁迫条件下穿心莲生长的影响
Pub Date : 2020-02-12 DOI: 10.5281/APS.2020.9.2.4
A. K. Sharan, V. Chandra
The effect of Vermicompost on a medicinal plant of great economic significance has been monitored. The plant Andrographis paniculata L. of family Lamiaceae is widely used all over the world for its biologically active ingredients containing diterpenoid, flavonoids, and polyphenols. The effect of Chlorpyrifos along with Vermicompost has been monitored on this plant. On one hand treatment of Vermicompost has been made to stimulate the growth and development of the test plant, an attempt has also been made to study the growth of the test plant under the stress condition of Chlorpyrifos. It has been noticed that the germination of the test plant increases with the lapse of time in the presence of Vermicompost as 100% growth is attained in 10 days. This value is 42.9% without Vermicompost during the same period of time. Low concentration of Chlorpyrifos (01%) also favors germination up to the extent of 50% in presence of Vermicompost the value is 25% in presence of (01%) of Chlorpyrifos but without Vermicompost. There has been marked reduction in the rate of germination in presence of 02% of Chlorpyrifos in the atmosphere of Vermicompost the germination is reduced by 25% this is further reduced to 15% in 03% of Chlorpyrifos. This value has been 20% and 10% in the presence of 02% and 03% of Chlorpyrifos in presence of Vermicompost. Plant height has also been taken into account for such type of treatment and it shows that with a lapse of time the plant height increases with 15% of Vermicompost. This rising trend continues even in 01%, 02% and 03% of Chlorpyrifos. An increase in the number of leaves means an increase in productivity. A similar trend of increase as described in germination and plant height has been also recorded in the emergence of leaves. With the lapse of time, the number increases during various treatments made. Test plants under treatment exhibited different kinds of morphological variations. As a prelude leaves of different treatments were used for calculating the area of leaves. This feature has also been found to be similar to the results described above i.e. with the increase in days of treatment the area of the leaf increases. The result described above has been presented below.
研究了蚯蚓堆肥对一种具有重要经济意义的药用植物的作用。穿心莲科穿心莲属植物穿心莲因其含有二萜类、黄酮类和多酚类生物活性成分而被广泛应用于世界各地。观察了毒死蜱与蚯蚓堆肥在该植物上的施用效果。一方面对蚯蚓堆肥进行了处理,刺激了被试植株的生长发育,也尝试研究了毒死蜱胁迫条件下被试植株的生长情况。已经注意到,在蚯蚓堆肥存在的情况下,试验植物的发芽随着时间的推移而增加,在10天内达到100%的生长。在同一时间内,如果不使用蚯蚓堆肥,该值为42.9%。低浓度毒死蜱(01%)在蚯蚓堆肥存在的情况下也有利于发芽率达50%,在毒死蜱(01%)不存在蚯蚓堆肥的情况下萌发率为25%。在蚯蚓堆肥的气氛中,含有02%毒死蜱时,发芽率明显降低,发芽率降低25%,在含有03%毒死蜱时,发芽率进一步降低至15%。在含有02%和03%毒死蜱的蚯蚓堆肥中,该值分别为20%和10%。这种处理也考虑了植株高度,结果表明,随着时间的推移,植株高度增加了15%的蚯蚓堆肥。即使在01%、02%和03%的毒死蜱中,这种上升趋势仍在继续。叶子数量的增加意味着生产力的提高。在萌发和株高方面也有类似的增加趋势,在叶片的出现方面也有记录。随着时间的推移,在各种处理过程中,数量增加。不同处理的试验植株表现出不同的形态变异。作为前奏,用不同处理的叶片计算叶片面积。这一特征也被发现与上面描述的结果相似,即随着处理天数的增加,叶子的面积增加。下面介绍了上述结果。
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引用次数: 0
Agro-morphological characterization of rice (Oryza sativa L.) landraces of Lamjung and Tanahun District, Nepal. 尼泊尔Lamjung和Tanahun地区地方稻(Oryza sativa L.)的农业形态特征。
Pub Date : 2020-02-03 DOI: 10.5281/APS.2020.9.2.3
Shishir Sharma, Amrit Pokhrel, Anup Dhakal, A. Poudel
Agro-morphological characterization of crop is the basic criteria to provide fundamental information for plant breeding programs. An investigation was conducted in Agronomy farm of Institute of Agriculture and Animal Science, Lamjung Campus in alpha-lattice design with two replications. The objective of the study was to characterize thirty landraces of rice ( Oryza sativa L.) of Lamjung and Tanahun district based on 30 qualitative and 8 quantitative characters, recorded as per descriptor established by IRRI, Bioversity International and WARDA. The frequency distribution of qualitative characters were studied where panicle shattering, lemma & palea color, culm lodging resistance and Leaf senescence showed high variability. The maximum value, minimum value, coefficient of variation and Pearson correlation were analyzed to study quantitative characters. The coefficient of variation ranged from 5.4% (Grain Length: Breadth ratio) to 20% (Total Tillers) indicating that selection based on the characters showing higher variation is expected to be effective. The highest correlation was corresponded to the total tillers and effective tillers (r=0.978 ** ). Flag leaf length showed positive significant correlation with penultimate leaf length (r=0.674 ** ), total tillers (r=0.437 ** ), effective tillers (r=0.356 ** ) and Grain Length: Breadth ratio (r=0.430 ** ). UPGMA clustering of the genotypes was done where members of cluster IV were found to be more superior. Promising landraces were identified from different yield attributing characters like total tillers, total grains per panicle, 1000 grain weight, panicle length and filled grain percentage. Thus, the present study can be utilized for further rice improvement programs and can also be used for assessing genetic diversity among morphologically distinguishable rice landraces.
作物的农业形态特征是为植物育种计划提供基础信息的基本标准。在农畜科学研究所兰亭校区的农艺场进行了2个重复的阿尔法格设计调查。本研究利用IRRI、Bioversity International和WARDA建立的描述子记录的30个定性性状和8个定量性状,对Lamjung和Tanahun地区的30个地方水稻(Oryza sativa L.)进行了表征。研究了穗碎裂、外稃颜色、茎秆抗倒伏和叶片衰老等品质性状的频率分布。分析其最大值、最小值、变异系数和Pearson相关性,研究其数量性状。变异系数在5.4%(粒长宽比)~ 20%(总分蘖数)之间,表明变异较大的性状选择是有效的。总分蘖数与有效分蘖数相关性最高(r=0.978 **)。旗叶长与倒数叶长(r=0.674 **)、总分蘖数(r=0.437 **)、有效分蘖数(r=0.356 **)、粒长宽比(r=0.430 **)呈极显著正相关。UPGMA对基因型进行聚类,其中发现簇IV的成员更优越。从分蘖数、每穗总粒数、千粒重、穗长和结实率等不同产量属性特征来鉴定有潜力的地方品种。因此,本研究可用于进一步的水稻改良计划,也可用于评估形态上可区分的水稻地方品种的遗传多样性。
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引用次数: 6
Growth and yield performance of oyster mushroom (Pleurotus ostreatus) on water hyacinth as a substrate 以水葫芦为底物的平菇生长及产量性能研究
Pub Date : 2020-02-03 DOI: 10.5281/APS.2020.9.2.1
Sanjay Kumar Jha, Menuka Gotame
Cultivation of oyster mushroom ( Pleurotus ostreatus ) on the aquatic weed water hyacinth is an eco-friendly way of controlling and management of such a problematic weed. In the present work, water hyacinth has been used as a low-cost substrate in combinations with rice-straw of ratio 3:1 for the cultivation of  P. ostreatus . The objective of this study was to cultivate oyster mushroom in water hyacinth compost degraded with lingo-cellulolytic fungi. The experiment was performed in control, before fungal treated compost and fungal treated compost. The data was analyzed on various aspects like completion of mycelium growth in different media like Potato dextrose agar media and water hyacinth media, the duration for spawn run, the appearance of pinheads, fruiting bodies and number of fruit bodies produced. The nutrient analysis was done in all types of fruit bodies.  Aspergillus flavus  treated compost took short duration (24 ± 1 days) and control sample compost took a long duration (27.3 ± 1.53 days) for fruiting. The highest production was recorded in  Trichoderma  sp. treated compost (68.8%) and lowest in control compost (30.7%). This study has successfully demonstrated the possibility of water hyacinth as a substrate in mushroom production and management of water hyacinth.
在水葫芦上种植平菇(Pleurotus ostreatus)是控制和管理这一问题杂草的一种生态友好的方式。本研究以水葫芦为低成本基质,与稻秆以3:1的比例组合,栽培黄绿假单胞菌。本研究的目的是在降解纤维素真菌降解的水葫芦堆肥中培养平菇。试验采用对照、真菌处理堆肥前和真菌处理堆肥。对不同培养基(如马铃薯葡萄糖琼脂培养基和水葫芦培养基)菌丝生长完成情况、产卵持续时间、针头外观、子实体和产生的子实体数量等方面进行了数据分析。对各类型果体进行了营养分析。黄曲霉处理堆肥出菇时间短(24±1 d),对照堆肥出菇时间长(27.3±1.53 d)。木霉处理堆肥产量最高(68.8%),对照堆肥产量最低(30.7%)。本研究成功地证明了水葫芦作为菌类生产和管理基质的可能性。
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引用次数: 0
Comments on the typification of two names in Arundinaria (Poaceae) 小檗属(禾科)中两个名称的类型性评价
Pub Date : 2020-02-03 DOI: 10.5281/APS.2020.9.2.2
Ananda Kumar
The paper deals with the second-step lectotypification of two names, Arundinaria maling Gamble and A. pantlingii Gamble.
本文讨论了Arundinaria maling Gamble和A. pantlingii Gamble两个名称的第二步lectyp分类法。
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引用次数: 0
e-flora of CCS University, Meerut, Uttar Pradesh, India. 印度北方邦密鲁特CCS大学的e-flora。
Pub Date : 2020-01-06 DOI: 10.5281/APS.2020.9.1.1
K. Varsha, Vijai Malik
A comprehensive study on the digitization of dendroids of CCS University, Meerut (UP) for the preparation of e-flora has been carried out during 2018-19. This e-flora is the first systematic approach for the digitization of dendroids in the Meerut region of Western Uttar Pradesh. It is a web-based program in which one can dynamically browse botanical name, vernacular name, family, and can search by name, distributional data, brief description, diagnostic features and images of all the dendroids located in the CCS University, Meerut ( http://efloraofccsumeerut.epizy.com/?i=1 ). This study and web tool include 223 species of dendroids along with more than 1,133 images.
在2018- 2019年期间,对密鲁特CCS大学(UP)的树状体数字化进行了全面研究,以制备e-flora。这种电子植物群是西部北方邦密鲁特地区树突植物数字化的第一个系统方法。这是一个基于网络的程序,在这个程序中,人们可以动态浏览植物名称、本地名称、科,并可以按名称、分布数据、简要描述、诊断特征和图像搜索位于密鲁特CCS大学的所有树突(http://efloraofccsumeerut.epizy.com/?i=1)。该研究和网络工具包括223种树突植物以及超过1133张图像。
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引用次数: 0
Evaluation of pumpkin genotypes (Cucurbita moschata) suitable for summer season 适合夏季种植的南瓜基因型评价
Pub Date : 2020-01-06 DOI: 10.5281/APS.2020.9.1.3
V. Krishnamoorthy, S. Sampath
The present investigation was carried out at Department of Horticulture, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai during summer 2016 with 32 pumpkin ( Cucurbita moschata Duch. ex. Poir) collected from different parts of Tamil Nadu to identify small fruited high quality genotypes for summer season. The genotypes CM-9, CM 3, CM 14 (3.40) recorded more number of primary branches, the highest vine length was recorded in CM18, CM10, CM15 (10.66m, 10.50m, 9.67m) genotypes. The higher petioles were recorded in CM21, CM18, CM4 (26.63, 24.77, 24.50cm). The higher leaf length was recorded in CM5, CM11, CM1, CM9, CM8, CM23, CM2, CM31 (14.03 to 14.93cm) genotypes. The leaf breadth was highest in CM15, (22.00cm) and the higher inter nodal length in CM17 (13.53cm) was recorded. The first male flower in seventh node was observed in CM23, CM1, CM32, CM30, CM4, the first female flower appeared early in CM12, CM26, (20 and 21 st node), the days taken for male flowering was ranged from 47.20 to 54.20 days and for flowering ranged from 51.73 to 63.27 days. The early days to female flowering was recorded in CM29, CM20 and CM17. The lowest sex ratio was recorded in CM30 (13.58). The days taken for fruit maturity less in CM29, CM28 and CM5 (83.47, 87.27 and 87.53 days). The genotypes CM29, CM28 recorded higher number of fruits per plant (4.40 and 3.13) and less fruit diameter (17.80 and 18.33cm), fruit length (44.40 and 49.73 cm), fruit weight (0.97 and 1.11kg), 100 seed weight (10 and 15g). The highest yield per plant was recorded in CM12, CM23, CM3 (9.48, 8.54 and 7.60kg). The highest TSS content (10 brix) in CM13, acidity (0.78) in CM27, ascorbic acid (10mg) in CM23, moisture content (99%) in CM30, CM31, beta carotene content in CM29, and CM28 (0.99 and 0.89 mg). The CM29 and CM28 genotypes were identified as small fruited type among the genotypes studied.
本调查于2016年夏季在马杜莱泰米尔纳德邦农业大学农学院和研究所园艺系进行,共32个南瓜(Cucurbita moschata Duch)。例如,从泰米尔纳德邦不同地区收集的Poir),以确定夏季小果高质量基因型。CM-9、CM 3、CM 14(3.40)一次枝数最多,CM18、CM10、CM15 (10.66m、10.50m、9.67m)一次枝数最多。较高叶柄在CM21、CM18、CM4分别为26.63、24.77、24.50cm;CM5、CM11、CM1、CM9、CM8、CM23、CM2、CM31 (14.03 ~ 14.93cm)的叶长较高。其中CM15的叶宽最高,为22.00cm, CM17的节间长最高,为13.53cm。CM23、CM1、CM32、CM30、CM4在第7节出现第一朵雄花,CM12、CM26(第20、21节)较早出现第一朵雌花,雄花开花时间为47.20 ~ 54.20 d,开花时间为51.73 ~ 63.27 d。CM29、CM20和CM17均有雌花前期的记录。性别比最低的是CM30(13.58)。CM29、CM28和CM5的果实成熟期较短,分别为83.47、87.27和87.53 d。基因型CM29、CM28单株果数(4.40和3.13)较高,果径(17.80和18.33cm)、果长(44.40和49.73 cm)、果重(0.97和1.11kg)和百粒重(10和15g)较低。单株产量最高的品种为CM12、CM23和CM3(9.48、8.54和7.60kg)。其中,TSS含量最高的是CM13 (10 brix),酸度最高的是CM27(0.78),抗坏血酸最高的是CM23 (10mg),水分含量最高的是CM30、CM31 (99%), β -胡萝卜素含量最高的是CM29、CM28(0.99、0.89 mg)。在研究的基因型中,CM29和CM28基因型被确定为小果型。
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引用次数: 0
Activity test of crude extracts of invasive plants Ageratina adenophora and Ipomoea carnea ssp. fistulosa against human pathogenic bacteria 入侵植物刺槐和野牡丹粗提物的活性测定。瘘管菌抗人类致病菌
Pub Date : 2020-01-06 DOI: 10.5281/APS.2020.9.1.4
Ritu Das, A. Devkota
The aqueous (distilled water) and alcoholic (methanol) crude extract from the leaves of invasive alien plant species Ageratina adenophora and Ipomoea carnea ssp. fistulosa were evaluated against six human pathogenic bacteria, three Gram negative: Escherichia coli (ATCC 25922) , Klebsiella pneumoniaea (ATCC 15380) and Proteus mirabilis (ATCC 49132) and three Gram positive: Enterococcus faecalis (ATCC 29212) Bacillus subtilis (ATCC 6633) and Staphylococcus aureus (ATCC 25923) using disc diffusion method. Different concentrations of plant extracts (50 mg/ml, 100 mg/ml, 150 mg/ml, 200 mg/ml and 250 mg/ml) were applied and diameter of zone of inhibition (ZOI) of bacterial growth were recorded. Both plant extracts showed antibacterial activity against Gram positive and Gram-negative bacteria. Methanolic extract of plant extracts exhibited good activity against tested bacteria when compared to aqueous extract. Among tested plants I. carnea ssp . fistula was more active than A. adenophora . The zone of inhibition of bacterial growth increased with increasing concentrations. The demonstration of activity against all these organisms had shown that both alien invasive species; Ageratina adenophora and Ipomoea carnea ssp. fistulosa. can be used to produce raw materials/substances for further development of diverse antibiotics with broad spectrum of activity.
研究了外来入侵植物Ageratina adenophora和Ipomoea carnea ssp的水(蒸馏水)和醇(甲醇)粗提物。采用圆盘扩散法对6种人致病菌进行检测,其中革兰氏阴性3种:大肠杆菌(ATCC 25922)、肺炎克雷伯菌(ATCC 15380)和奇异变形杆菌(ATCC 49132),革兰氏阳性3种:粪肠球菌(ATCC 29212)、枯草芽孢杆菌(ATCC 6633)和金黄色葡萄球菌(ATCC 25923)。应用不同浓度的植物提取物(50 mg/ml、100 mg/ml、150 mg/ml、200 mg/ml和250 mg/ml),记录细菌生长的抑制区直径(ZOI)。两种植物提取物对革兰氏阳性菌和革兰氏阴性菌均有抑菌活性。植物提取物的甲醇提取物与水提取物相比,具有较好的抑菌活性。在受试植物中;瘘管比腺状假体更活跃。细菌生长的抑制区随着浓度的增加而增大。对所有这些生物的活性证明,外来入侵物种;紫叶菊和紫叶菊。fistulosa。可用于生产原料/物质,用于进一步开发具有广谱活性的各种抗生素。
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引用次数: 1
A taxonomic revision of the genus Eriolaena (Malvaceae) in India. 标题印度凤仙花属的分类订正。
Pub Date : 2020-01-06 DOI: 10.5281/APS.2020.9.1.2
K. Chandramohan, Y. Mahesh, K. Rambabu, E. Kiran
A detailed work on genus Eriolaena archives all names published in a concise nomenclatural review. It is derived from the tribe Dombeya with specific limitations and under the generic delimitation. Detailed taxonomic information & descriptions of all taxa and photographs have been provided for easy identification.
一个详细的工作,对所有的名称发表在一个简明的命名综述。它起源于Dombeya部落,有特定的限制和属界。为方便鉴定,提供了详细的分类资料和所有分类群的描述和照片。
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引用次数: 1
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Annals of Plant Sciences
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