Nikola Kokanov, Milena Krajnovic, Snezana Cupic-Jovanovic, Bojana Kožik, N. Petrović, A. Božović, V. Mandušić
Prevention of chronic hepatitis C (CHC) and its complications is based on antiviral therapy and early detection of reliable molecular markers in persons under risk. We investigated whether the methylation status of RASSF1A and p16 genes, alone or in combination with host and viral factors, affects the response to therapy with pegylated interferon/ribavirin (PEGIFN/ RBV). Methylation-specific polymerase chain reaction (MSP) was used to determine the methylation status of the target promoter sequences of RASSF1A and p16 in circulating-free DNA from the peripheral blood of 49 patients with CHC genotype 1b. The methylation status of the examined genes did not affect the response to therapy. However, the simultaneous presence of either RASSF1A or p16 methylation and the CC genotype of IL28B was significantly related to a sustained virologic response (P=0.009 and P=0.032, respectively). After Bonferroni correction, only the result concerning the RASSF1A gene remained significant (P<0.0125). Methylation of RASSF1A was associated with the CC genotype of the IL28B gene (P=0.024) and a higher viral load (?400 000 IU/mL, P=0.009). Our results suggest that combined analysis of RASSF1A gene methylation and IL28B rs12979860 polymorphism could potentially help in the prediction of therapy response in CHC genotype 1b patients.
{"title":"RASSF1A and p16 promoter methylation and treatment response in chronic hepatitis C genotype 1b patients treated with pegylated interferon/ribavirin","authors":"Nikola Kokanov, Milena Krajnovic, Snezana Cupic-Jovanovic, Bojana Kožik, N. Petrović, A. Božović, V. Mandušić","doi":"10.2298/abs211208004k","DOIUrl":"https://doi.org/10.2298/abs211208004k","url":null,"abstract":"Prevention of chronic hepatitis C (CHC) and its complications is based on antiviral therapy and early detection of reliable molecular markers in persons under risk. We investigated whether the methylation status of RASSF1A and p16 genes, alone or in combination with host and viral factors, affects the response to therapy with pegylated interferon/ribavirin (PEGIFN/ RBV). Methylation-specific polymerase chain reaction (MSP) was used to determine the methylation status of the target promoter sequences of RASSF1A and p16 in circulating-free DNA from the peripheral blood of 49 patients with CHC genotype 1b. The methylation status of the examined genes did not affect the response to therapy. However, the simultaneous presence of either RASSF1A or p16 methylation and the CC genotype of IL28B was significantly related to a sustained virologic response (P=0.009 and P=0.032, respectively). After Bonferroni correction, only the result concerning the RASSF1A gene remained significant (P<0.0125). Methylation of RASSF1A was associated with the CC genotype of the IL28B gene (P=0.024) and a higher viral load (?400 000 IU/mL, P=0.009). Our results suggest that combined analysis of RASSF1A gene methylation and IL28B rs12979860 polymorphism could potentially help in the prediction of therapy response in CHC genotype 1b patients.","PeriodicalId":8145,"journal":{"name":"Archives of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68389951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Jovanović, Sladjana Popovic, G. Subakov-Simić, V. Jovanovic, D. Predojević, D. Jovanović, V. Karadzic
Herein we provide an assessment of cyanobacterial diversity and habitat preferences of potentially toxic and alien taxa, which could be an important tool for human health risk assessment regarding recreational and water-supply waterbodies. The diversity changes of cyanobacteria in waters intended for human consumption in Serbia were analyzed two decades after the first floristic study was published. The examination included phytoplankton and phytobenthic sample analysis from 35 localities in the period between 2012 and 2017, together with published literature records. The results indicate that the number of identified taxa doubled since the first Serbian Flora of Cyanobacteria was released two decades ago. The changes most likely occurred due to environmental factors, including hydrological transformations of habitats, cultural eutrophication and global warming. Many frequently recorded taxa are potentially toxic and bloom-forming. The spread of alien species with potentially invasive characteristics has also been noted. Canonical correspondence analysis (CCA) indicates that shallow waterbodies are the most vulnerable regarding the occurrence and expansion of bloom-forming, potentially toxic and invasive taxa. This shows the urgent need for a more detailed investigation. Additionally, although most of the research was focused on planktonic forms, benthic cyanobacteria represent an important component for public health risk assessment and therefore should be more frequently investigated.
{"title":"Freshwater cyanobacteria in waters intended for human consumption in Serbia: Two decades of changes in diversity","authors":"J. Jovanović, Sladjana Popovic, G. Subakov-Simić, V. Jovanovic, D. Predojević, D. Jovanović, V. Karadzic","doi":"10.2298/abs220518020j","DOIUrl":"https://doi.org/10.2298/abs220518020j","url":null,"abstract":"Herein we provide an assessment of cyanobacterial diversity and habitat preferences of potentially toxic and alien taxa, which could be an important tool for human health risk assessment regarding recreational and water-supply waterbodies. The diversity changes of cyanobacteria in waters intended for human consumption in Serbia were analyzed two decades after the first floristic study was published. The examination included phytoplankton and phytobenthic sample analysis from 35 localities in the period between 2012 and 2017, together with published literature records. The results indicate that the number of identified taxa doubled since the first Serbian Flora of Cyanobacteria was released two decades ago. The changes most likely occurred due to environmental factors, including hydrological transformations of habitats, cultural eutrophication and global warming. Many frequently recorded taxa are potentially toxic and bloom-forming. The spread of alien species with potentially invasive characteristics has also been noted. Canonical correspondence analysis (CCA) indicates that shallow waterbodies are the most vulnerable regarding the occurrence and expansion of bloom-forming, potentially toxic and invasive taxa. This shows the urgent need for a more detailed investigation. Additionally, although most of the research was focused on planktonic forms, benthic cyanobacteria represent an important component for public health risk assessment and therefore should be more frequently investigated.","PeriodicalId":8145,"journal":{"name":"Archives of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68390200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Brankovic, G. Stanojevic, A. Veljković, G. Kocić, M. Nestorović, B. Djindjic, J. Bašić, I. Stojanovic
Colorectal cancer (CRC) is one of the most frequent forms of malignant tumors in the human population. The literature data about the role of arginine metabolism in CRC point out its double-faced role. In three tissue specimens of 50 patients who underwent surgical resection for colon adenocarcinoma (tumor, adjacent and healthy tissues more than 10 cm from the tumor border - at the incision margin) taken during surgery, polyamines and the concentration of NO2+NO3 and arginase activity were determined. Polyamine levels and arginase activity were significantly increased in cancer and adjacent tissue specimens compared to healthy ones, while the level of NO2+NO3 was significantly lower in cancer compared to both adjacent and healthy tissues. The high polyamine content in the adjacent colonic mucosa indicates a high proliferative potential of tumor-adjacent tissue. Although we found individual correlations indicating the possible prognostic value of arginase, the performed statistical analysis did not show a predictive significance of arginase activity in the examined tissue specimens for five-year survival of the patients. Nevertheless, the obtained results provide the rationale for further studies of arginine metabolism in tissue specimens after surgery in patients with CRC, which could be useful in the evaluation of the risk for tumor growth, recurrence, metastases and survival after surgical intervention.
{"title":"Altered arginine metabolism in colon cancer: A sign of increased proliferative potential of tumor-adjacent tissue","authors":"B. Brankovic, G. Stanojevic, A. Veljković, G. Kocić, M. Nestorović, B. Djindjic, J. Bašić, I. Stojanovic","doi":"10.2298/abs220531023b","DOIUrl":"https://doi.org/10.2298/abs220531023b","url":null,"abstract":"Colorectal cancer (CRC) is one of the most frequent forms of malignant tumors in the human population. The literature data about the role of arginine metabolism in CRC point out its double-faced role. In three tissue specimens of 50 patients who underwent surgical resection for colon adenocarcinoma (tumor, adjacent and healthy tissues more than 10 cm from the tumor border - at the incision margin) taken during surgery, polyamines and the concentration of NO2+NO3 and arginase activity were determined. Polyamine levels and arginase activity were significantly increased in cancer and adjacent tissue specimens compared to healthy ones, while the level of NO2+NO3 was significantly lower in cancer compared to both adjacent and healthy tissues. The high polyamine content in the adjacent colonic mucosa indicates a high proliferative potential of tumor-adjacent tissue. Although we found individual correlations indicating the possible prognostic value of arginase, the performed statistical analysis did not show a predictive significance of arginase activity in the examined tissue specimens for five-year survival of the patients. Nevertheless, the obtained results provide the rationale for further studies of arginine metabolism in tissue specimens after surgery in patients with CRC, which could be useful in the evaluation of the risk for tumor growth, recurrence, metastases and survival after surgical intervention.","PeriodicalId":8145,"journal":{"name":"Archives of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68390220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Prostate cancer (PCa) is a common type of cancer worldwide. The incidence of PCa increases with age and it is the most common malignant tumor in men. Tissue biopsy and the serum prostatespecific antigen are still the standards for diagnosing suspected PCa. Long non-coding RNA (lncRNA) contributes to the progression of PCa by recruiting transcriptional regulators. We utilized highthroughput sequencing data and bioinformatics analysis to identify specifically expressed lncRNAs in PCa and filtered out three specific lncRNAs for further analysis: AC077690.1, AL049874.3 and AP001037.1. We constructed a lncRNA regulatory network and used differentially expressed mRNA interactions to predict the functions of the selected lncRNAs. Functional enrichment analysis and PCR verification of these three lncRNAs revealed that they were closely related to well-known PI3K-AktmTOR and the forkhead box protein (FOXO) signaling pathways involved in PCa. By understanding the related interactions between these molecules and signaling pathways, the lncRNAs could be potential candidates for therapeutic targets in PCa.
{"title":"Differentially expressed AC077690.1, AL049874.3 and AP001037.1 lncRNAs in prostate cancer","authors":"Hexin Li, Xiaokun Tang, Gaoyuan Sun, Siyuan Xu, Luyao Wang, Lanxin Zhang, Ya-qun Zhang, Fei Su, Lili Zhang, Wei Zhang","doi":"10.2298/abs221025034l","DOIUrl":"https://doi.org/10.2298/abs221025034l","url":null,"abstract":"Prostate cancer (PCa) is a common type of cancer worldwide. The incidence of PCa increases with age and it is the most common malignant tumor in men. Tissue biopsy and the serum prostatespecific antigen are still the standards for diagnosing suspected PCa. Long non-coding RNA (lncRNA) contributes to the progression of PCa by recruiting transcriptional regulators. We utilized highthroughput sequencing data and bioinformatics analysis to identify specifically expressed lncRNAs in PCa and filtered out three specific lncRNAs for further analysis: AC077690.1, AL049874.3 and AP001037.1. We constructed a lncRNA regulatory network and used differentially expressed mRNA interactions to predict the functions of the selected lncRNAs. Functional enrichment analysis and PCR verification of these three lncRNAs revealed that they were closely related to well-known PI3K-AktmTOR and the forkhead box protein (FOXO) signaling pathways involved in PCa. By understanding the related interactions between these molecules and signaling pathways, the lncRNAs could be potential candidates for therapeutic targets in PCa.","PeriodicalId":8145,"journal":{"name":"Archives of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68390547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Few studies have examined the usefulness of soluble apoptotic markers for the screening of pleural effusion. This study aimed to investigate the significance of Fas, tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and fibrinolytic factors for the assessment of patients with malignant pleural effusion. A total of 137 patients with pleural effusion were evaluated. Soluble Fas, TRAIL, tissue-type plasminogen activator (tPA), plasminogen activator inhibitor-1 (PAI-1), D-dimers and lactate dehydrogenase (LD) levels were measured. Pleural fluid/blood ratios (P/B) of fibrinolytic factors were calculated. Fas and TRAIL levels were significantly higher in patients with malignant effusion than in those with non-malignant effusion. Malignant effusion was 1.6-fold more prevalent in patients with elevated Fas than in those without (48.5% vs 30.4%, P=0.031). The P/B ratio of tPA was 2.5-fold higher in malignant effusion than in non-malignant effusion (4.65 vs 1.83, P<0.001). Fas was positively correlated with tPA and D-dimers, but not with biochemical parameters. The ability of Fas to identify malignant effusions was significantly greater than those of tPA and LD. In conclusion, measurements of Fas and TRAIL in conjunction with fibrinolytic factors may provide information useful for monitoring patients with suspected malignant pleural effusion.
很少有研究检查可溶性凋亡标志物在筛选胸腔积液中的作用。本研究旨在探讨Fas、肿瘤坏死因子相关凋亡诱导配体(tumor necrosis factor- associated apoptosis-inducing ligand, TRAIL)和纤溶因子在恶性胸腔积液患者评估中的意义。我们对137例胸腔积液患者进行了评估。测定可溶性Fas、TRAIL、组织型纤溶酶原激活物(tPA)、纤溶酶原激活物抑制剂-1 (PAI-1)、d -二聚体和乳酸脱氢酶(LD)水平。计算胸水/血纤溶因子比(P/B)。Fas和TRAIL水平在恶性积液患者中明显高于非恶性积液患者。Fas升高患者的恶性积液发生率是无Fas升高患者的1.6倍(48.5% vs 30.4%， P=0.031)。恶性积液中tPA的P/B比非恶性积液高2.5倍(4.65 vs 1.83, P<0.001)。Fas与tPA和d -二聚体呈正相关，但与生化参数无关。Fas识别恶性胸腔积液的能力明显高于tPA和LD。总之，Fas和TRAIL结合纤溶因子的测量可能为监测疑似恶性胸腔积液患者提供有用的信息。
{"title":"The significance of Fas, tumor necrosis factor-related apoptosis-inducing ligand and fibrinolytic factors in the assessment of malignant pleural effusion","authors":"J. Choi, Moon-Shin Lee, T. Fujii","doi":"10.2298/abs220316010c","DOIUrl":"https://doi.org/10.2298/abs220316010c","url":null,"abstract":"Few studies have examined the usefulness of soluble apoptotic markers for the screening of pleural effusion. This study aimed to investigate the significance of Fas, tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and fibrinolytic factors for the assessment of patients with malignant pleural effusion. A total of 137 patients with pleural effusion were evaluated. Soluble Fas, TRAIL, tissue-type plasminogen activator (tPA), plasminogen activator inhibitor-1 (PAI-1), D-dimers and lactate dehydrogenase (LD) levels were measured. Pleural fluid/blood ratios (P/B) of fibrinolytic factors were calculated. Fas and TRAIL levels were significantly higher in patients with malignant effusion than in those with non-malignant effusion. Malignant effusion was 1.6-fold more prevalent in patients with elevated Fas than in those without (48.5% vs 30.4%, P=0.031). The P/B ratio of tPA was 2.5-fold higher in malignant effusion than in non-malignant effusion (4.65 vs 1.83, P<0.001). Fas was positively correlated with tPA and D-dimers, but not with biochemical parameters. The ability of Fas to identify malignant effusions was significantly greater than those of tPA and LD. In conclusion, measurements of Fas and TRAIL in conjunction with fibrinolytic factors may provide information useful for monitoring patients with suspected malignant pleural effusion.","PeriodicalId":8145,"journal":{"name":"Archives of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68389784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Bašić, V. Milošević, Milica Živanović, Jasen Kundalić, M. Despotović, T. Jevtović-Stoimenov, I. Stojanovic
Although genetic variations rs780094 and rs1260326 of the glucokinase regulatory protein gene (GCKR) could be associated with lipid profile imbalance, their influence on acute ischemic stroke (AIS) risk has not yet been established. The aim of this study was to investigate the influence of GCKR single nucleotide polymorphisms (SNPs) rs780094 and rs1260326 on lipid profile parameters in patients with AIS, and to evaluate the association of these SNPs with the risk of AIS. In a casecontrol study, a total of 148 subjects were screened for GCKR rs780094 and rs1260326 SNPs using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The lipid profile was determined based on serum total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C) and triacylglycerol (TG) concentrations. The frequencies of the minor rs780094T allele and the minor rs1260326T allele were significantly lower in AIS patients compared to controls. The rs780094TT genotype and the rs1260326TT genotype were associated with decreased risk of AIS compared to wildtype carriers. In conclusion, this is the first study implying that decreased risk of AIS in rs780094 and rs1260326 homozygous minor allele carriers is not caused by dyslipidemia, but possibly by the lack of coagulation factor glycosylation.
{"title":"Association of rs780094 and rs1260326 glucokinase regulatory protein gene polymorphisms with dyslipidemia in a group of Serbian acute ischemic stroke patients","authors":"J. Bašić, V. Milošević, Milica Živanović, Jasen Kundalić, M. Despotović, T. Jevtović-Stoimenov, I. Stojanovic","doi":"10.2298/abs211126002b","DOIUrl":"https://doi.org/10.2298/abs211126002b","url":null,"abstract":"Although genetic variations rs780094 and rs1260326 of the glucokinase regulatory protein gene (GCKR) could be associated with lipid profile imbalance, their influence on acute ischemic stroke (AIS) risk has not yet been established. The aim of this study was to investigate the influence of GCKR single nucleotide polymorphisms (SNPs) rs780094 and rs1260326 on lipid profile parameters in patients with AIS, and to evaluate the association of these SNPs with the risk of AIS. In a casecontrol study, a total of 148 subjects were screened for GCKR rs780094 and rs1260326 SNPs using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The lipid profile was determined based on serum total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C) and triacylglycerol (TG) concentrations. The frequencies of the minor rs780094T allele and the minor rs1260326T allele were significantly lower in AIS patients compared to controls. The rs780094TT genotype and the rs1260326TT genotype were associated with decreased risk of AIS compared to wildtype carriers. In conclusion, this is the first study implying that decreased risk of AIS in rs780094 and rs1260326 homozygous minor allele carriers is not caused by dyslipidemia, but possibly by the lack of coagulation factor glycosylation.","PeriodicalId":8145,"journal":{"name":"Archives of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68389940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Bisko, M. Lomberg, O. Mykchaylova, N. Mytropolska, V. Kutovenko, A. Gryganskyi
Effective methods of preserving the gene pool of valuable edible and medicinal mushrooms are to maintain them in in vitro culture collections and to correctly identify and verify the preserved strains. It is necessary to consider not only the results of molecular genetic studies but also cultural, morphological and physiological characteristics as additional criteria. This article presents data on the colony and mycelial morphology, growth characteristics and temperature tolerance, and phylogenetical placement of four strains of edible and medicinal mushroom from the P. eryngii species complex received into the IBK Mushroom Collection as P. nebrodensis strains. All the studied strains are mesophiles with the fastest growth rate of 11.0 mm/day at 26?? and a lethal temperature of 40??. In addition to common anastomoses, mycelial strands and clamp connections, the vegetative mycelium of the studied strains formed single colorless round excretory cells on the lateral hyphal ramifications. All cultures were able to form primordia and fruit bodies on agar media. Phylogenetic analysis suggests that all four strains do not belong to P. nebrodensis, but two of them, IBK 1947, 2035 are P. eryngii var. ferulae, and two strains, IBK 1855 and 1927, belong to P. tuoliensis (P. eryngii var. tuoliensis).
保存有价值的食用菌和药用菌基因库的有效方法是在离体培养基中保存菌种，并对保存菌种进行正确的鉴定和验证。不仅要考虑分子遗传学研究的结果，还要考虑文化、形态和生理特征作为附加标准。本文介绍了4株可食用和药用蘑菇的菌落和菌丝形态、生长特性和耐温性，以及作为nebrodensis菌株进入IBK蘑菇收藏的4株可食用和药用蘑菇的系统发育位置。所有菌株均为嗜中菌，在26℃时生长速度最快，为11.0 mm/d。而致命的温度是40度?所研究菌株的营养菌丝除了常见的吻合、丝链和钳状连接外，还在菌丝侧分枝上形成单个无色圆形排泄细胞。所有培养物均能在琼脂培养基上形成原基和子实体。系统发育分析表明，4株病原菌均不属于nebrodensis，但其中2株ibk1947、2035属于P. eryngii var. ferulae, 2株ibk1855、1927属于P. tuoliensis (P. eryngii var. tuoliensis)。
{"title":"Biology, morphology, and phylogeny of some strains of the Pleurotus eryngii species complex","authors":"N. Bisko, M. Lomberg, O. Mykchaylova, N. Mytropolska, V. Kutovenko, A. Gryganskyi","doi":"10.2298/abs220524026b","DOIUrl":"https://doi.org/10.2298/abs220524026b","url":null,"abstract":"Effective methods of preserving the gene pool of valuable edible and medicinal mushrooms are to maintain them in in vitro culture collections and to correctly identify and verify the preserved strains. It is necessary to consider not only the results of molecular genetic studies but also cultural, morphological and physiological characteristics as additional criteria. This article presents data on the colony and mycelial morphology, growth characteristics and temperature tolerance, and phylogenetical placement of four strains of edible and medicinal mushroom from the P. eryngii species complex received into the IBK Mushroom Collection as P. nebrodensis strains. All the studied strains are mesophiles with the fastest growth rate of 11.0 mm/day at 26?? and a lethal temperature of 40??. In addition to common anastomoses, mycelial strands and clamp connections, the vegetative mycelium of the studied strains formed single colorless round excretory cells on the lateral hyphal ramifications. All cultures were able to form primordia and fruit bodies on agar media. Phylogenetic analysis suggests that all four strains do not belong to P. nebrodensis, but two of them, IBK 1947, 2035 are P. eryngii var. ferulae, and two strains, IBK 1855 and 1927, belong to P. tuoliensis (P. eryngii var. tuoliensis).","PeriodicalId":8145,"journal":{"name":"Archives of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68390210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Snake venom fibrinogenolytic enzymes have diagnostic and therapeutic value and are important for snakebite pathology. In the present study, the fibrinogenolytic activity of Montivipera raddei venom was investigated. Crude venom was incubated with human fibrinogen for different times at 37?C. An inhibition study was carried out using different protease inhibitors. The fibrinogenolytic activity was assessed by SDS-PAGE and fibrinogen zymography. An HPLC-based method was used to obtain confirmatory data. Montivipera raddei venom predominantly cleaved the A? chain of fibrinogen in a time-dependent manner. A very slight decrease in band intensity of the B? chain was observable after a longer incubation time. Cleavage of fibrinogen was confirmed by HPLC. Zymography revealed that the venom contained 50 and 75 kDa fibrinogenolytic enzymes. Ethylenediaminetetraacetic acid (EDTA) and 1,10-phenanthroline inhibited the overall fibrinogenolytic activity, while phenylmethylsulfonyl fluoride (PMSF) and aprotinin only inhibited the degradation of the B? chain. These results indicated that metalloproteinases were major fibrinogenolytic enzymes in the venom. An inhibitor study suggested the presence of serine proteinases that broke down the B? chain. With this study, the fibrinogenolytic activity of M. raddei venom was shown for the first time. The results will be useful for further isolation and characterization studies.
{"title":"Determination of the fibrinogenolytic activity of Montivipera raddei (Raddeʼs mountain viper) venom","authors":"Fikriye Atasoy, Naşit Iğci","doi":"10.2298/abs220806029a","DOIUrl":"https://doi.org/10.2298/abs220806029a","url":null,"abstract":"Snake venom fibrinogenolytic enzymes have diagnostic and therapeutic value and are important for snakebite pathology. In the present study, the fibrinogenolytic activity of Montivipera raddei venom was investigated. Crude venom was incubated with human fibrinogen for different times at 37?C. An inhibition study was carried out using different protease inhibitors. The fibrinogenolytic activity was assessed by SDS-PAGE and fibrinogen zymography. An HPLC-based method was used to obtain confirmatory data. Montivipera raddei venom predominantly cleaved the A? chain of fibrinogen in a time-dependent manner. A very slight decrease in band intensity of the B? chain was observable after a longer incubation time. Cleavage of fibrinogen was confirmed by HPLC. Zymography revealed that the venom contained 50 and 75 kDa fibrinogenolytic enzymes. Ethylenediaminetetraacetic acid (EDTA) and 1,10-phenanthroline inhibited the overall fibrinogenolytic activity, while phenylmethylsulfonyl fluoride (PMSF) and aprotinin only inhibited the degradation of the B? chain. These results indicated that metalloproteinases were major fibrinogenolytic enzymes in the venom. An inhibitor study suggested the presence of serine proteinases that broke down the B? chain. With this study, the fibrinogenolytic activity of M. raddei venom was shown for the first time. The results will be useful for further isolation and characterization studies.","PeriodicalId":8145,"journal":{"name":"Archives of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68390463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
We analyzed the economic benefits versus safety risks of sharing anti-vascular endothelial growth factor (VEGF) vials during the coronavirus disease (COVID-19) pandemic. This single-center retrospective study analyzed the data of patients with neovascular age-related macular degeneration (nAMD), proliferative diabetic retinopathy (PDR) and retinal vein occlusion (RVO) who received anti- VEGF between January 2016 and July 2021 at Renmin Hospital, Wuhan University, China. Costs were compared of the two protocols of intravitreal injections (IVIs) of ranibizumab, aflibercept and conbercept after (i) splitting the vial content for use in two patients and after (ii) disposal of the remaining vial content after use in a single patient, with the COVID-19 outbreak considered as the demarcation point. The incidence rates of post-injection endophthalmitis (PIE) pre- and post-outbreak were analyzed. The mean cost of a single IVI increased by 33.3%, from 3917.67?71.69 to 5222.67?84.98 Chinese Yuan during the pandemic. The incidences of IVI-related culture-positive PIE were 0.0134% (3 in 22448) and 0.0223% (1 in 4479), respectively, before and after the pandemic (P=0.6532). We conclude that vial sharing of IVIs in a large clinical institution is not associated with increased PIE risk and can significantly reduce the cost of therapy.
{"title":"Impact of the COVID-19 pandemic on patients receiving intravitreal injections","authors":"Ningzhi Zhang, Xuejun He, Y. Xing, Ning Yang","doi":"10.2298/abs220116003z","DOIUrl":"https://doi.org/10.2298/abs220116003z","url":null,"abstract":"We analyzed the economic benefits versus safety risks of sharing anti-vascular endothelial growth factor (VEGF) vials during the coronavirus disease (COVID-19) pandemic. This single-center retrospective study analyzed the data of patients with neovascular age-related macular degeneration (nAMD), proliferative diabetic retinopathy (PDR) and retinal vein occlusion (RVO) who received anti- VEGF between January 2016 and July 2021 at Renmin Hospital, Wuhan University, China. Costs were compared of the two protocols of intravitreal injections (IVIs) of ranibizumab, aflibercept and conbercept after (i) splitting the vial content for use in two patients and after (ii) disposal of the remaining vial content after use in a single patient, with the COVID-19 outbreak considered as the demarcation point. The incidence rates of post-injection endophthalmitis (PIE) pre- and post-outbreak were analyzed. The mean cost of a single IVI increased by 33.3%, from 3917.67?71.69 to 5222.67?84.98 Chinese Yuan during the pandemic. The incidences of IVI-related culture-positive PIE were 0.0134% (3 in 22448) and 0.0223% (1 in 4479), respectively, before and after the pandemic (P=0.6532). We conclude that vial sharing of IVIs in a large clinical institution is not associated with increased PIE risk and can significantly reduce the cost of therapy.","PeriodicalId":8145,"journal":{"name":"Archives of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68389994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study investigated the effect of microRNA mir-548ar-3p on cigarette smoke extract (CSE)-induced chronic obstructive pulmonary disease (COPD). High-throughput sequencing was performed on peripheral blood from smoking COPD patients and non-smoking individuals with normal pulmonary function, and mir-548ar-3p RNA, possessing large differential expression was selected. Experimental groups were divided into control, experimental model (EM), EM+mimic miRNA, negative control (NC) and EM+miR-548ar-3p groups; an empty vector or miR-548ar-3p mimic was transfected into human bronchial epithelial (HBE) cells. A COPD model was established by treating HBE cells with CSE. Cell viability, apoptosis and solute carrier family 17 member 9 (SLC17A9) protein expression were examined by cell counting kit-8, flow cytometry and Western blotting, respectively. Cell viability in the EM+miR-548ar-3p group decreased significantly, and the apoptosis rate and SLC17A9 protein expression increased significantly compared with the control (P<0.05, all groups). In smoking COPD patients, interferon (IFN)-? and interleukin (IL)-17? expression detected by ELISA was significantly higher than in normal individuals. miR-548ar-3p expression was significantly lower (P<0.05, all groups). These findings suggest that miR-548ar-3p was expressed at a lower level in COPD patients. miR-548ar-3p may increase the extent of CSE-induced COPD injury through SLC17A9.
{"title":"MIR-548ar-3p increases cigarette smoke extractinduced chronic obstructive pulmonary disease (COPD) injury through solute carrier family 17 member 9 (SLC17A9)","authors":"Longju Zhang, Xiaoli Liu, Z. Yi, Fei Du, G. He","doi":"10.2298/abs220201008z","DOIUrl":"https://doi.org/10.2298/abs220201008z","url":null,"abstract":"This study investigated the effect of microRNA mir-548ar-3p on cigarette smoke extract (CSE)-induced chronic obstructive pulmonary disease (COPD). High-throughput sequencing was performed on peripheral blood from smoking COPD patients and non-smoking individuals with normal pulmonary function, and mir-548ar-3p RNA, possessing large differential expression was selected. Experimental groups were divided into control, experimental model (EM), EM+mimic miRNA, negative control (NC) and EM+miR-548ar-3p groups; an empty vector or miR-548ar-3p mimic was transfected into human bronchial epithelial (HBE) cells. A COPD model was established by treating HBE cells with CSE. Cell viability, apoptosis and solute carrier family 17 member 9 (SLC17A9) protein expression were examined by cell counting kit-8, flow cytometry and Western blotting, respectively. Cell viability in the EM+miR-548ar-3p group decreased significantly, and the apoptosis rate and SLC17A9 protein expression increased significantly compared with the control (P<0.05, all groups). In smoking COPD patients, interferon (IFN)-? and interleukin (IL)-17? expression detected by ELISA was significantly higher than in normal individuals. miR-548ar-3p expression was significantly lower (P<0.05, all groups). These findings suggest that miR-548ar-3p was expressed at a lower level in COPD patients. miR-548ar-3p may increase the extent of CSE-induced COPD injury through SLC17A9.","PeriodicalId":8145,"journal":{"name":"Archives of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68390008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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