This study investigated the effect of microRNA mir-548ar-3p on cigarette smoke extract (CSE)-induced chronic obstructive pulmonary disease (COPD). High-throughput sequencing was performed on peripheral blood from smoking COPD patients and non-smoking individuals with normal pulmonary function, and mir-548ar-3p RNA, possessing large differential expression was selected. Experimental groups were divided into control, experimental model (EM), EM+mimic miRNA, negative control (NC) and EM+miR-548ar-3p groups; an empty vector or miR-548ar-3p mimic was transfected into human bronchial epithelial (HBE) cells. A COPD model was established by treating HBE cells with CSE. Cell viability, apoptosis and solute carrier family 17 member 9 (SLC17A9) protein expression were examined by cell counting kit-8, flow cytometry and Western blotting, respectively. Cell viability in the EM+miR-548ar-3p group decreased significantly, and the apoptosis rate and SLC17A9 protein expression increased significantly compared with the control (P<0.05, all groups). In smoking COPD patients, interferon (IFN)-? and interleukin (IL)-17? expression detected by ELISA was significantly higher than in normal individuals. miR-548ar-3p expression was significantly lower (P<0.05, all groups). These findings suggest that miR-548ar-3p was expressed at a lower level in COPD patients. miR-548ar-3p may increase the extent of CSE-induced COPD injury through SLC17A9.
{"title":"MIR-548ar-3p increases cigarette smoke extractinduced chronic obstructive pulmonary disease (COPD) injury through solute carrier family 17 member 9 (SLC17A9)","authors":"Longju Zhang, Xiaoli Liu, Z. Yi, Fei Du, G. He","doi":"10.2298/abs220201008z","DOIUrl":"https://doi.org/10.2298/abs220201008z","url":null,"abstract":"This study investigated the effect of microRNA mir-548ar-3p on cigarette smoke extract (CSE)-induced chronic obstructive pulmonary disease (COPD). High-throughput sequencing was performed on peripheral blood from smoking COPD patients and non-smoking individuals with normal pulmonary function, and mir-548ar-3p RNA, possessing large differential expression was selected. Experimental groups were divided into control, experimental model (EM), EM+mimic miRNA, negative control (NC) and EM+miR-548ar-3p groups; an empty vector or miR-548ar-3p mimic was transfected into human bronchial epithelial (HBE) cells. A COPD model was established by treating HBE cells with CSE. Cell viability, apoptosis and solute carrier family 17 member 9 (SLC17A9) protein expression were examined by cell counting kit-8, flow cytometry and Western blotting, respectively. Cell viability in the EM+miR-548ar-3p group decreased significantly, and the apoptosis rate and SLC17A9 protein expression increased significantly compared with the control (P<0.05, all groups). In smoking COPD patients, interferon (IFN)-? and interleukin (IL)-17? expression detected by ELISA was significantly higher than in normal individuals. miR-548ar-3p expression was significantly lower (P<0.05, all groups). These findings suggest that miR-548ar-3p was expressed at a lower level in COPD patients. miR-548ar-3p may increase the extent of CSE-induced COPD injury through SLC17A9.","PeriodicalId":8145,"journal":{"name":"Archives of Biological Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68390008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zorana Hrkic-Ilic, M. Borišev, L. Zorić, D. Arsenov, J. Luković
An anatomical study of adventitious roots of three Salix clones, B-44, SV068 and SM4041, treated with 3 and 6 mg Cd kg-1 dry weight in soil in a greenhouse experiment. The aim was to analyze the anatomical characteristics of roots in response to pollution by cadmium and to assess the potential application of anatomical and hydraulic characteristics in the selection of the most suitable Salix clones for phytostabilization of pollutants in soils. Anatomical parameters measured in this study included root crosssectional area, root diameter, the proportion of periderm, secondary phloem (cortex) and wood (secondary and primary xylem), and parameters of the vessels (lumen area, diameter and frequency). Based on the measurements of individual vessel lumens and the number of vessels, the theoretical hydraulic conductivity (kh) of roots was calculated. The effects of applied Cd concentrations on root traits were studied in clones and control plants. Following treatments with both Cd concentrations, plants of clone B-44 had the highest values of most parameters and significantly higher kh in comparison with control samples due to the significantly larger root cross-sectional area and lumen of vessels. It was concluded that these characteristics can serve for effective evaluation and selection of clones for remediation of sites contaminated with cadmium.
3和6 mg Cd kg-1干重处理下柳B-44、SV068和SM4041三个无性系不定根的解剖研究。目的是分析柳树根系对镉污染响应的解剖特征，并评估解剖和水力特征在选择最适合土壤中污染物植物稳定的柳树无性系中的潜在应用。本研究测量的解剖学参数包括根横截面积、根直径、周皮、次生韧皮部(皮层)和木材(次生和初生木质部)的比例以及血管参数(管腔面积、直径和频率)。通过对单个管腔和管数的测量，计算了根的理论导水系数kh。研究了施用镉浓度对无性系和对照植株根系性状的影响。在两种Cd浓度处理下，无性系B-44植株的根横截面积和管腔均显著增大，大部分参数值最高，kh值显著高于对照。这些特征可为镉污染场地修复无性系的评价和选择提供有效依据。
{"title":"Assessment of differences in anatomical and hydraulic properties of the root and xylem of three willow (Salix L.) clones during phytostabilization after exposure to elevated cadmium","authors":"Zorana Hrkic-Ilic, M. Borišev, L. Zorić, D. Arsenov, J. Luković","doi":"10.2298/abs220309016h","DOIUrl":"https://doi.org/10.2298/abs220309016h","url":null,"abstract":"An anatomical study of adventitious roots of three Salix clones, B-44, SV068 and SM4041, treated with 3 and 6 mg Cd kg-1 dry weight in soil in a greenhouse experiment. The aim was to analyze the anatomical characteristics of roots in response to pollution by cadmium and to assess the potential application of anatomical and hydraulic characteristics in the selection of the most suitable Salix clones for phytostabilization of pollutants in soils. Anatomical parameters measured in this study included root crosssectional area, root diameter, the proportion of periderm, secondary phloem (cortex) and wood (secondary and primary xylem), and parameters of the vessels (lumen area, diameter and frequency). Based on the measurements of individual vessel lumens and the number of vessels, the theoretical hydraulic conductivity (kh) of roots was calculated. The effects of applied Cd concentrations on root traits were studied in clones and control plants. Following treatments with both Cd concentrations, plants of clone B-44 had the highest values of most parameters and significantly higher kh in comparison with control samples due to the significantly larger root cross-sectional area and lumen of vessels. It was concluded that these characteristics can serve for effective evaluation and selection of clones for remediation of sites contaminated with cadmium.","PeriodicalId":8145,"journal":{"name":"Archives of Biological Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68389728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Szekeres, A. Beermann, T. Neubauer, Miroslav Očadlík, M. Paunović, Maja Rakovic, B. Csányi, A. Varga, A. Weigand, T. Wilke, Z. Fehér
The distribution and spread of a new alien gastropod species, Clathrocaspia knipowitschii (Makarov, 1938) (Gastropoda: Hydrobiidae: Caspiinae) in the Danube River was examined. First findings of this species for Hungary, Slovakia, Romania, Bulgaria and Serbia are presented. Clathrocaspia knipowitschii was initially found in 2013 in the Iron Gate stretch of the Danube River at the border between Romania and Serbia. In 2019 and 2020, the species was found at several sites in the lower Danube in Romania, Serbia and Bulgaria, and also upstream in the middle Hungarian Danube in high population densities. The species appears to have spread along more than 800 km in six years. This finding together with the available abundance data indicates that C. knipowitschii is potentially an invasive species, but further observations are needed.
{"title":"Rapid spread of a new alien and potentially invasive species, Clathrocaspia knipowitschii (Makarov, 1938) (Gastropoda: Hydrobiidae), in the Danube River","authors":"J. Szekeres, A. Beermann, T. Neubauer, Miroslav Očadlík, M. Paunović, Maja Rakovic, B. Csányi, A. Varga, A. Weigand, T. Wilke, Z. Fehér","doi":"10.2298/abs220211006s","DOIUrl":"https://doi.org/10.2298/abs220211006s","url":null,"abstract":"The distribution and spread of a new alien gastropod species, Clathrocaspia knipowitschii (Makarov, 1938) (Gastropoda: Hydrobiidae: Caspiinae) in the Danube River was examined. First findings of this species for Hungary, Slovakia, Romania, Bulgaria and Serbia are presented. Clathrocaspia knipowitschii was initially found in 2013 in the Iron Gate stretch of the Danube River at the border between Romania and Serbia. In 2019 and 2020, the species was found at several sites in the lower Danube in Romania, Serbia and Bulgaria, and also upstream in the middle Hungarian Danube in high population densities. The species appears to have spread along more than 800 km in six years. This finding together with the available abundance data indicates that C. knipowitschii is potentially an invasive species, but further observations are needed.","PeriodicalId":8145,"journal":{"name":"Archives of Biological Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68390071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Retinoic acid receptor ? (RAR?) is a transcription factor that plays an essential role in tumor progression. Triple-negative breast cancer (TNBC) is a subtype of breast carcinoma with a poor prognosis due to early therapeutic escape from conventional treatments and aggressive metastatic relapse by the occurrence of an epithelial-mesenchymal transition (EMT). However, as the expression level of RAR? does not correlate with the overall survival of TNBC patients, we speculate that post-translational modification such as phosphorylation of RAR? may be involved in EMT and TNBC metastasis. After overexpressing a phosphorylation-defective mutant of RAR? at serine 77 residue (RAR?S77A), we found that RAR? hypophosphorylation inhibited MDA-MB-231 cell motility and migration in vitro while reducing the lung metastatic potential in vivo. This was accompanied by increased expression of the epithelial marker E-cadherin and decreased expression of the mesenchymal markers ?-catenin and zinc finger E-box-binding homeobox 1 (ZEB1) in agreement with the suppression of EMT. Interestingly, the overexpression of wild-type RAR? in the presence of the RAR? agonist AM580 failed to suppress EMT and cell migration. These results indicate that hypophosphorylated RAR?S77 can directly mimic activated RAR? to inhibit EMT and migration/invasion of cells, thus providing a novel target in the therapeutic intervention of TNBC.
{"title":"Hypophosphorylation of retinoic acid receptor alpha inhibits triple-negative breast cancer cell migration and invasion","authors":"Jiajia Ying, Fanli Zheng, Yanan Zheng, Hongtao Hu, Siyue Lou","doi":"10.2298/abs220531025y","DOIUrl":"https://doi.org/10.2298/abs220531025y","url":null,"abstract":"Retinoic acid receptor ? (RAR?) is a transcription factor that plays an essential role in tumor progression. Triple-negative breast cancer (TNBC) is a subtype of breast carcinoma with a poor prognosis due to early therapeutic escape from conventional treatments and aggressive metastatic relapse by the occurrence of an epithelial-mesenchymal transition (EMT). However, as the expression level of RAR? does not correlate with the overall survival of TNBC patients, we speculate that post-translational modification such as phosphorylation of RAR? may be involved in EMT and TNBC metastasis. After overexpressing a phosphorylation-defective mutant of RAR? at serine 77 residue (RAR?S77A), we found that RAR? hypophosphorylation inhibited MDA-MB-231 cell motility and migration in vitro while reducing the lung metastatic potential in vivo. This was accompanied by increased expression of the epithelial marker E-cadherin and decreased expression of the mesenchymal markers ?-catenin and zinc finger E-box-binding homeobox 1 (ZEB1) in agreement with the suppression of EMT. Interestingly, the overexpression of wild-type RAR? in the presence of the RAR? agonist AM580 failed to suppress EMT and cell migration. These results indicate that hypophosphorylated RAR?S77 can directly mimic activated RAR? to inhibit EMT and migration/invasion of cells, thus providing a novel target in the therapeutic intervention of TNBC.","PeriodicalId":8145,"journal":{"name":"Archives of Biological Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68390274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F1012-2, a novel sesquiterpene lactone isolated from the Chinese herbal medicine Eupatorium lindleyanum DC, exhibits an antitumor effect. In this study, we investigated the anticancer activities of F1012-2 on ten human breast cancer lines and demonstrated significantly lower IC50 values for triple-negative breast cancer (TNBC) than for non-TNBC cell lines. The transcription factors p53 and nuclear factor-?B (NF-?B) are important regulators of tumorigenesis. F1012-2 not only depleted mutant p53, but also activated wild-type p53. F1012-2 reduced the expression of phosphorylated p65 and p105 F-?B family members and coregulated p53, NF-?B members and their dependent targets. To further clarify the key role of p53, lentivirus small hairpin RNA (shRNA) infection was used to knockdown p53 in MDAMB- 231 cells. F1012-2 significantly reduced the inhibitory effect on cell proliferation and apoptosis, while the levels of p53, NF-?B family members and their dependent genes were not significantly different. F1012-2 exhibited a significant antitumor effect and reduced the expression of p53 in MDA-MB-231 xenografts. Taken together, our results show that F1012-2 exhibited an inhibitory effect on TNBC and affected the regulation of p53/NF-?B signaling pathways.
{"title":"Eupatorium lindleyanum DC. sesquiterpene fraction F1012-2 regulates p53/NF-κB signaling pathways in human breast cancer","authors":"Xuepeng Wang, Jiajin Xu, Yixuan Tao, Xiangrong Fan, Xintong Shen, S. Tian","doi":"10.2298/abs220630028w","DOIUrl":"https://doi.org/10.2298/abs220630028w","url":null,"abstract":"F1012-2, a novel sesquiterpene lactone isolated from the Chinese herbal medicine Eupatorium lindleyanum DC, exhibits an antitumor effect. In this study, we investigated the anticancer activities of F1012-2 on ten human breast cancer lines and demonstrated significantly lower IC50 values for triple-negative breast cancer (TNBC) than for non-TNBC cell lines. The transcription factors p53 and nuclear factor-?B (NF-?B) are important regulators of tumorigenesis. F1012-2 not only depleted mutant p53, but also activated wild-type p53. F1012-2 reduced the expression of phosphorylated p65 and p105 F-?B family members and coregulated p53, NF-?B members and their dependent targets. To further clarify the key role of p53, lentivirus small hairpin RNA (shRNA) infection was used to knockdown p53 in MDAMB- 231 cells. F1012-2 significantly reduced the inhibitory effect on cell proliferation and apoptosis, while the levels of p53, NF-?B family members and their dependent genes were not significantly different. F1012-2 exhibited a significant antitumor effect and reduced the expression of p53 in MDA-MB-231 xenografts. Taken together, our results show that F1012-2 exhibited an inhibitory effect on TNBC and affected the regulation of p53/NF-?B signaling pathways.","PeriodicalId":8145,"journal":{"name":"Archives of Biological Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68390360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. Likic-Ladjevic, Dejana Nešić, A. Stefanović, S. Kadija, Z. Vilendečić, I. Pilić, J. Dotlic, M. Radojevic, B. Milošević, K. Stefanovic
The study aimed to assess the oncologic and reproductive outcome of fertility-sparing treatment of stage I borderline ovarian tumors (BOTs). A retrospective study of patients aged 18-40 years with stage I BOTs surgically treated during a 10-year period was conducted. In total, 52 patients (average age 32.7+/-5.9) were followed for 16 to 137 months after BOT diagnosis (mean 73.4 months). The overall survival rate was 100%. Recurrence was registered in 4 patients (7.7%). All patients underwent a unilateral salpingo-oophorectomy and all histologic findings corresponded with primary BOTs (3 serous; 1 endometrioid). Higher parity increased while the histological type and stage did not impact recurrence. Average recurrence-free survival was 36.2+/-23.6 months regardless of histological type and stage. Recurrence occurred during the first 3 postoperative years in 75% of cases. Out of 45 women treated with fertility-sparing surgery, 64.4% attempted pregnancy and the pregnancy success rate was 44.8%. Only one pregnancy was conceived by assisted reproduction, while all others were spontaneous. The fertilitysparing treatment in stage I borderline ovarian tumors can be a safe and successful option both in terms of oncologic and reproductive outcomes, regardless of patient and treatment characteristics. Pregnancies after BOT surgery can be achieved with satisfactory rates and adequate outcomes.
{"title":"Oncologic and reproductive outcomes of fertility-sparing surgery for borderline ovarian tumors: First presentation of 10-year experience from a Serbian referral center","authors":"I. Likic-Ladjevic, Dejana Nešić, A. Stefanović, S. Kadija, Z. Vilendečić, I. Pilić, J. Dotlic, M. Radojevic, B. Milošević, K. Stefanovic","doi":"10.2298/abs220722027l","DOIUrl":"https://doi.org/10.2298/abs220722027l","url":null,"abstract":"The study aimed to assess the oncologic and reproductive outcome of fertility-sparing treatment of stage I borderline ovarian tumors (BOTs). A retrospective study of patients aged 18-40 years with stage I BOTs surgically treated during a 10-year period was conducted. In total, 52 patients (average age 32.7+/-5.9) were followed for 16 to 137 months after BOT diagnosis (mean 73.4 months). The overall survival rate was 100%. Recurrence was registered in 4 patients (7.7%). All patients underwent a unilateral salpingo-oophorectomy and all histologic findings corresponded with primary BOTs (3 serous; 1 endometrioid). Higher parity increased while the histological type and stage did not impact recurrence. Average recurrence-free survival was 36.2+/-23.6 months regardless of histological type and stage. Recurrence occurred during the first 3 postoperative years in 75% of cases. Out of 45 women treated with fertility-sparing surgery, 64.4% attempted pregnancy and the pregnancy success rate was 44.8%. Only one pregnancy was conceived by assisted reproduction, while all others were spontaneous. The fertilitysparing treatment in stage I borderline ovarian tumors can be a safe and successful option both in terms of oncologic and reproductive outcomes, regardless of patient and treatment characteristics. Pregnancies after BOT surgery can be achieved with satisfactory rates and adequate outcomes.","PeriodicalId":8145,"journal":{"name":"Archives of Biological Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68390407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aquatic ecosystems are recipients of various contaminants including pesticides. For many years, pyrethroid insecticides (e.g., tau-fluvalinate) have been used extensively in agricultural activities to control pests. However, they can affect not only target organisms but also non-target organisms. This study was conducted to investigate the lethal and sublethal effects of tau-fluvalinate on the non-target organism Gammarus roeseli. To this end, acute toxicity of tau-fluvalinate was determined using a toxicity test with a 96-h exposure period, and the genotoxic effects of different sublethal concentrations on hemocytes of the test organism were assessed at 24-, 96-, and 240-h exposure periods using the comet assay. Alterations in locomotor activity of the test organism in response to exposure to sublethal concentrations were evaluated at 120- and 240-h periods. The 96-h median lethal concentration (LC50) was found to be 17.29 ?g/L, and tau-fluvalinate was observed to cause a significant increase in DNA damage and a significant reduction in locomotor activity at the tested sublethal concentrations (2.15, 4.30 and 8.60 ?g/L). The results of this study suggest that the long-term existence of tau-fluvalinate in aquatic environments at high concentrations is a noteworthy threat to non-target organisms and that its use in agricultural activities should be reconsidered.
{"title":"Lethal and sublethal effects of the pyrethroid insecticide tau-fluvalinate on the non-target organism Gammarus roeseli: A study of acute toxicity, genotoxicity and locomotor activity","authors":"F. Sari","doi":"10.2298/abs220930033s","DOIUrl":"https://doi.org/10.2298/abs220930033s","url":null,"abstract":"Aquatic ecosystems are recipients of various contaminants including pesticides. For many years, pyrethroid insecticides (e.g., tau-fluvalinate) have been used extensively in agricultural activities to control pests. However, they can affect not only target organisms but also non-target organisms. This study was conducted to investigate the lethal and sublethal effects of tau-fluvalinate on the non-target organism Gammarus roeseli. To this end, acute toxicity of tau-fluvalinate was determined using a toxicity test with a 96-h exposure period, and the genotoxic effects of different sublethal concentrations on hemocytes of the test organism were assessed at 24-, 96-, and 240-h exposure periods using the comet assay. Alterations in locomotor activity of the test organism in response to exposure to sublethal concentrations were evaluated at 120- and 240-h periods. The 96-h median lethal concentration (LC50) was found to be 17.29 ?g/L, and tau-fluvalinate was observed to cause a significant increase in DNA damage and a significant reduction in locomotor activity at the tested sublethal concentrations (2.15, 4.30 and 8.60 ?g/L). The results of this study suggest that the long-term existence of tau-fluvalinate in aquatic environments at high concentrations is a noteworthy threat to non-target organisms and that its use in agricultural activities should be reconsidered.","PeriodicalId":8145,"journal":{"name":"Archives of Biological Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68390534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This is a notice of retraction of the article: High resolution karyotype of Thai crab-eating macaque (Macaca fascicularis) by Xiaobo Fan, Alongkoad Tanomtong, Arunrat Chaveerach, Krit Pinthong, Siripiyasing Pornnarong, Weerayuth Supiwong, Thomas Liehr, and Anja Weise, published in: Archives of Biological Sciences in 2014, Vol. 66, Issue 4: https://doi.org/10.2298/ABS1404603F. The Editor-in-Chief has been informed that this paper is a duplicate of the paper: High resolution karyotype of Thai crab-eating macaque (Macaca fascicularis) by Xiaobo Fan, Alongkoad Tanomtong, Arunrat Chaveerach, Krit Pinthong, Siripiyasing Pornnarong, Weerayuth Supiwong, Thomas Liehr, Anja Weise, published in: Genetika, Vol. 46, No. 3, 877- 882, 2014: https://doi.org/10.2298/GENSR1403877F. This claim is correct and the entire paper is a verbatim copy of the earlier one. After confirmation of this fact, the Editor-in-Chief of the Archives of Biological Sciences has decided to retract the paper immediately. We apologize to the readers of the journal that it took so many years to notice this error and to retract the paper. We request readers of the journal to directly get in touch with the editorial office and the editors of the journal for similar cases in the future so that they can be handled promptly. Link to the retracted article 10.2298/ABS1404603F
{"title":"Retraction: Fan X, Tanomtong A, Chaveerach A, Pinthong K, Pornnarong S, Supiwong W, Liehr T, Weise A. High resolution karyotype of Thai crab-eating macaque (Macaca fascicularis). Arch Biol Sci. 2014;66(4):1603-7. Doi: 10.2298/ABS1404603F","authors":"E. Editorial","doi":"10.2298/abs220504012e","DOIUrl":"https://doi.org/10.2298/abs220504012e","url":null,"abstract":"This is a notice of retraction of the article: High resolution karyotype of Thai crab-eating macaque (Macaca fascicularis) by Xiaobo Fan, Alongkoad Tanomtong, Arunrat Chaveerach, Krit Pinthong, Siripiyasing Pornnarong, Weerayuth Supiwong, Thomas Liehr, and Anja Weise, published in: Archives of Biological Sciences in 2014, Vol. 66, Issue 4: https://doi.org/10.2298/ABS1404603F. The Editor-in-Chief has been informed that this paper is a duplicate of the paper: High resolution karyotype of Thai crab-eating macaque (Macaca fascicularis) by Xiaobo Fan, Alongkoad Tanomtong, Arunrat Chaveerach, Krit Pinthong, Siripiyasing Pornnarong, Weerayuth Supiwong, Thomas Liehr, Anja Weise, published in: Genetika, Vol. 46, No. 3, 877- 882, 2014: https://doi.org/10.2298/GENSR1403877F. This claim is correct and the entire paper is a verbatim copy of the earlier one. After confirmation of this fact, the Editor-in-Chief of the Archives of Biological Sciences has decided to retract the paper immediately. We apologize to the readers of the journal that it took so many years to notice this error and to retract the paper. We request readers of the journal to directly get in touch with the editorial office and the editors of the journal for similar cases in the future so that they can be handled promptly. Link to the retracted article 10.2298/ABS1404603F","PeriodicalId":8145,"journal":{"name":"Archives of Biological Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68389926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
As oxidative stress is implicated in the pathogenesis of head and neck squamous cell cancer (HNSCC), the functions of antioxidant enzyme systems and DNA repair proteins are critical in the development of cancer. To investigate the role of genetic polymorphisms of the antioxidant superoxide dismutase 2 (SOD2) Val16Ala, glutathione peroxidase 1 (GPX1) Pro198Leu, and the DNA repair Xeroderma Pigmentosum Group D (XPD) Lys751Gln genes under exogenous risk factors, including smoking and alcohol consumption, in HNSCC carcinogenesis, we conducted a case-control study on 139 unrelated cases and 265 non-cancer controls. Polymorphisms were analyzed in additive, dominant and recessive genetic models, individually and in an interaction model. Carriers of the T allele of SOD2 were associated with an increased risk for HNSCC in the overall subgroups of males and smokers; similarly, the T allele of GPX1 was associated with elevated risk in the overall and smoker subgroup. A 12.47-fold increased risk was observed for the carriers of GPX1 TT, SOD2 CT and XPD CC genotypes for HNSCC. This is the first study presenting the potential roles of SOD2, GPX1 and XPD polymorphisms in interaction and under three genetic models in the development of HNSCC. The results suggest that these polymorphisms slightly modify the risk in HNSCC development individually but are significantly higher when they functioned and were evaluated together.
{"title":"Association between superoxide dismutase 2, glutathione peroxidase 1, xeroderma pigmentosum group d gene variations, and head and neck squamous cell cancer susceptibility","authors":"G. Köse, M. Demirbugen Oz, E. Cömert, H. Süzen","doi":"10.2298/abs220509017k","DOIUrl":"https://doi.org/10.2298/abs220509017k","url":null,"abstract":"As oxidative stress is implicated in the pathogenesis of head and neck squamous cell cancer (HNSCC), the functions of antioxidant enzyme systems and DNA repair proteins are critical in the development of cancer. To investigate the role of genetic polymorphisms of the antioxidant superoxide dismutase 2 (SOD2) Val16Ala, glutathione peroxidase 1 (GPX1) Pro198Leu, and the DNA repair Xeroderma Pigmentosum Group D (XPD) Lys751Gln genes under exogenous risk factors, including smoking and alcohol consumption, in HNSCC carcinogenesis, we conducted a case-control study on 139 unrelated cases and 265 non-cancer controls. Polymorphisms were analyzed in additive, dominant and recessive genetic models, individually and in an interaction model. Carriers of the T allele of SOD2 were associated with an increased risk for HNSCC in the overall subgroups of males and smokers; similarly, the T allele of GPX1 was associated with elevated risk in the overall and smoker subgroup. A 12.47-fold increased risk was observed for the carriers of GPX1 TT, SOD2 CT and XPD CC genotypes for HNSCC. This is the first study presenting the potential roles of SOD2, GPX1 and XPD polymorphisms in interaction and under three genetic models in the development of HNSCC. The results suggest that these polymorphisms slightly modify the risk in HNSCC development individually but are significantly higher when they functioned and were evaluated together.","PeriodicalId":8145,"journal":{"name":"Archives of Biological Sciences","volume":"22 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68390097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hui Yu, Lixia Zhang, Rina Su, Hai-ying Hu, Zhanli Wang
Klebsiella pneumoniae is an opportunistic pathogen that causes a wide range of infections. The emergence and spread of hypervirulent K. pneumoniae (hvKp), which appears to be different from the classical K. pneumoniae (cKp) in several microbiological aspects, is an urgent global threat. However, the virulence characteristics of hvKp and its differences from cKp are poorly understood. This work aimed to investigate the correlation between the expression characteristics of proteins and hypervirulence, using proteomics. Our results revealed that 185 proteins were upregulated while 266 proteins were downregulated in hvKp isolates when compared with cKp isolates. The differentially expressed proteins were functionally categorized according to the Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway. These proteins were predominantly metabolism associated, which indicates that changes in the metabolic pathways in hvKp isolates might in part contribute to hypervirulence.
{"title":"Altered metabolic pathways in classic and hypervirulent Klebsiella pneumoniae isolates revealed by proteomics analysis","authors":"Hui Yu, Lixia Zhang, Rina Su, Hai-ying Hu, Zhanli Wang","doi":"10.2298/abs220613022y","DOIUrl":"https://doi.org/10.2298/abs220613022y","url":null,"abstract":"Klebsiella pneumoniae is an opportunistic pathogen that causes a wide range of infections. The emergence and spread of hypervirulent K. pneumoniae (hvKp), which appears to be different from the classical K. pneumoniae (cKp) in several microbiological aspects, is an urgent global threat. However, the virulence characteristics of hvKp and its differences from cKp are poorly understood. This work aimed to investigate the correlation between the expression characteristics of proteins and hypervirulence, using proteomics. Our results revealed that 185 proteins were upregulated while 266 proteins were downregulated in hvKp isolates when compared with cKp isolates. The differentially expressed proteins were functionally categorized according to the Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway. These proteins were predominantly metabolism associated, which indicates that changes in the metabolic pathways in hvKp isolates might in part contribute to hypervirulence.","PeriodicalId":8145,"journal":{"name":"Archives of Biological Sciences","volume":"35 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68390345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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