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The influence of microwave-assisted osmotic dehydration in coconut meat preservation technique 微波辅助渗透脱水对椰肉保鲜技术的影响
Pub Date : 2024-07-15 DOI: 10.1016/j.afres.2024.100448
Mahfujul Alam , Mipahur Jannat , Nayon Datta , Mir Meahadi Hasan , Asraful Alam , Md. Ashrafuzzaman Zahid , Md. Shakir Moazzem , Mohammad Mainuddin Molla , Mrityunjoy Biswas

Coconut is cultivated in tropical and subtropical areas across the globe because of its diverse applications, particularly in terms of its nutritional and therapeutic benefits. In order to enhance the shelf life of coconut meat while maintaining its quality, it must be pre-processed. The research aimed to determine the physicochemical constituents, antioxidant properties, and sensory attributes of BARI-1 coconut meat preserved in various sugar solutions (40–80)% by thermal processing (boiling and microwave) during refrigeration storage (3 ± 1 °C) for 45 days. Based on the results of physicochemical characteristics (lightness L*, chroma C*, hue H*, ash, moisture, pH, TSS, fat, and fiber), antioxidant capabilities (DPPH and TPC) and sensory analysis, there were significant (p < 0.05) variations among the treatments. The coconut meat preserved in 80 % sugar solution with microwave treatment had significantly (p < 0.05) higher retention of the quality parameters compared to other treatments during storage. After being treated with 80 °Brix solution in a 900 W microwave for 5 mins, the phenolic content and antioxidant activity of the coconut meat were shown to be 106.96 mg GAE/100 g and 5.88 %, respectively. Based on multivariate statistical analyses, the quality of coconut meat is mostly influenced by thermal processing and storage period.There was a considerable rise in the bioactive compounds and a significant decrease in the moisture content observed during storage. Pearson's correlation analysis further validated the findings. After 45 days of storage, the microwave treated sample (M80) also had significantly (p < 0.05) higher scores (7.9) and boiling treated sample (B40) showed (6.5) least in overall acceptance in sensory analysis. Therefore, microwave treatment with an 80 % sugar solution was suggested for coconut meat preservation, which exhibited excellent nutritional and aesthetic stability during refrigeration storage. The findings are essential for producers, processors, and consumers looking to optimize the quality and functionality of coconut meat when utilized for multiple purposes.

椰子在全球热带和亚热带地区种植,因为它用途广泛,特别是具有营养和治疗功效。为了延长椰肉的保质期并保持其品质,必须对其进行预加工。这项研究旨在确定在冷藏储存(3 ± 1 °C)45 天期间,通过热处理(煮沸和微波)在各种糖溶液(40-80)%中保存的 BARI-1 椰子肉的理化成分、抗氧化特性和感官属性。根据理化特性(亮度 L*、色度 C*、色调 H*、灰分、水分、pH 值、总悬浮固体、脂肪和纤维)、抗氧化能力(DPPH 和 TPC)和感官分析的结果,各处理之间存在显著差异(p <0.05)。与其他处理相比,用微波处理 80 % 糖溶液保存的椰肉在贮藏期间的质量指标保持率明显更高(p < 0.05)。用 80 °Brix 溶液在 900 瓦微波炉中处理 5 分钟后,椰肉的酚含量和抗氧化活性分别为 106.96 毫克 GAE/100 克和 5.88%。根据多元统计分析,椰肉的质量主要受热加工和储存期的影响。皮尔逊相关分析进一步验证了这一结果。贮藏 45 天后,经微波处理的样品(M80)在感官分析中的总体接受度得分(7.9)也明显较高(p < 0.05),而煮沸处理的样品(B40)得分(6.5)最低。因此,建议用 80% 的糖溶液进行微波处理来保存椰子肉,在冷藏储存期间,椰子肉的营养和美观稳定性极佳。这些研究结果对生产商、加工商和消费者来说至关重要,因为他们希望在将椰肉用于多种用途时,优化椰肉的质量和功能。
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引用次数: 0
Developing a non-destructive method for the detection of egg quality and freshness using micro-Raman spectroscopy 开发一种利用微拉曼光谱检测鸡蛋质量和新鲜度的非破坏性方法
Pub Date : 2024-07-15 DOI: 10.1016/j.afres.2024.100453
Maryam Davari, Maryam Bahreini, Zahra Sabzevari

Considering the pivotal role of eggs in the food industry and their nutritional significance, this study employed micro-Raman spectroscopy of eggs, examining both shells and yolks to assess the quality and freshness of eggs. Raman spectra were collected at different temperatures and time intervals to investigate temperature and time effects, potentially indicating Raman peak reduction due to Maillard reaction and oxidation of proteins and lipids and carotenoid depletion, respectively. By calculating the ratio of Raman peaks, lipids, fatty acids, and choline methyl were introduced as biomarkers of temperature and time. Notable correlations were identified between Raman peaks and egg quality coefficients, including egg coefficient and peak 1002 cm−1 (protein), total weight and 1301 cm−1 (Lipids), yolk weight and 2934 and 3057 cm−1, total weight with peak 710 cm−1, and egg shape index and peak 3057 cm−1. Analysis of eggshells at different time intervals revealed Raman peak reduction during time, demonstrating Raman's effectiveness in assessing egg quality from its shell. Using the PLS-DA method, the classification of eggs at different temperatures and storage times using egg yolk Raman spectra was performed with 80 % accuracy that belongs to the validation report, predominantly influenced by carotenoid peaks, showing Raman a practical, non-destructive method for egg quality and freshness control.

考虑到鸡蛋在食品工业中的关键作用及其营养价值,本研究采用了鸡蛋的显微拉曼光谱,对蛋壳和蛋黄进行检测,以评估鸡蛋的质量和新鲜度。在不同的温度和时间间隔下采集拉曼光谱,以研究温度和时间的影响,这可能分别表明由于马氏反应、蛋白质和脂类的氧化以及类胡萝卜素的消耗而导致的拉曼峰值降低。通过计算拉曼峰的比率,引入了脂质、脂肪酸和甲基胆碱作为温度和时间的生物标记。拉曼峰与鸡蛋质量系数之间存在明显的相关性,包括鸡蛋系数与 1002 cm-1 峰(蛋白质)、总重量与 1301 cm-1(脂类)、蛋黄重量与 2934 和 3057 cm-1、总重量与 710 cm-1 峰、蛋形指数与 3057 cm-1 峰。对不同时间间隔的蛋壳进行分析后发现,拉曼峰值随时间推移而降低,这表明拉曼能有效地从蛋壳评估鸡蛋质量。使用 PLS-DA 方法,利用蛋黄拉曼光谱对不同温度和储存时间的鸡蛋进行分类,准确率达到 80%,属于验证报告,主要受类胡萝卜素峰的影响,表明拉曼是一种实用的、非破坏性的鸡蛋质量和新鲜度控制方法。
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引用次数: 0
Influence of banana powder on proximate composition, physicochemical and rheological properties of soy yoghurt 香蕉粉对大豆酸奶近似成分、理化和流变特性的影响
Pub Date : 2024-07-14 DOI: 10.1016/j.afres.2024.100450
Victor Vicent

The study investigates the impact of banana powder on the proximate composition, physicochemical, and rheological properties of soy yoghurt. The fermentation kinetics of soy yoghurt were assessed by monitoring the pH, which changed from 6.8 to 4.4 after 12 h. Matured banana fruits, ripened to stages 5 and 6 were used to enrich the soy yoghurt. The enriched soy yoghurt was stored at 4 °C for 28 days, during which its physicochemical and rheological properties were examined. The proximate composition and physicochemical properties of soy yoghurt were measured using standard methods. The proximate compositions showed significant differences (p < 0.05) among banana powder, soy yoghurt, and enriched soy yoghurt blends. Protein, fat, fibre, and ash contents in banana powder from stage 6 were significantly higher (p < 0.05) compared to those of stage 5. Moisture and fat contents decreased by 15 % to 19 % and 10 % to 12 %, respectively, when banana powder from stages 5 and 6 were added to soy yoghurt. The inclusion of banana powder into soy yoghurt led to a significant increase in protein, fibre, ash, and carbohydrate contents. Additionally, the total soluble solids (TSS) of enriched soy yoghurt significantly increased (p < 0.05) from 6 to 18 °Brix as the amount of banana powder increased and then decreased during storage. The pH values of soy yoghurt initially remained stable between 4.40 and 4.42 before gradually decreasing during 7 days of storage. The apparent viscosity increased as the amount of banana powder increased, indicating non-Newtonian fluid behaviour characterised by a shear-thinning property, as evidenced by n < 1. The study highlights the potential of banana powder to enhance the nutritional and rheological properties of soy yoghurt, thereby contributing to the development of plant-based dairy alternatives.

本研究探讨了香蕉粉对大豆酸奶的近似成分、物理化学和流变特性的影响。大豆酸奶的发酵动力学是通过监测 pH 值来评估的,12 小时后,pH 值从 6.8 变为 4.4。富集后的大豆酸奶在 4 °C 下储存 28 天,并在此期间检测其物理化学和流变特性。采用标准方法测量了大豆酸奶的近似物成分和理化性质。近似物成分在香蕉粉、大豆酸奶和富含大豆酸奶混合物之间存在显著差异(p < 0.05)。与第 5 阶段相比,第 6 阶段香蕉粉中的蛋白质、脂肪、纤维和灰分含量明显更高(p < 0.05)。在大豆酸奶中添加第 5 和第 6 阶段的香蕉粉时,水分和脂肪含量分别降低了 15 % 至 19 % 和 10 % 至 12 %。在大豆酸奶中加入香蕉粉后,蛋白质、纤维、灰分和碳水化合物的含量显著增加。此外,随着香蕉粉用量的增加,富集大豆酸奶的总可溶性固形物(TSS)从 6 °Brix 显著增加到 18 °Brix (p < 0.05),然后在贮藏过程中下降。大豆酸奶的 pH 值最初稳定在 4.40 至 4.42 之间,然后在 7 天的储存过程中逐渐降低。表观粘度随着香蕉粉用量的增加而增加,表明其具有剪切稀化特性的非牛顿流体行为,如 n < 1 所示。 该研究强调了香蕉粉增强大豆酸奶营养和流变特性的潜力,从而有助于开发植物乳制品替代品。
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引用次数: 0
Improvement of quality and shelf-life of tomatoes with Aloe vera coatings enriched with tulsi extract 用富含图尔西提取物的芦荟涂层改善西红柿的质量和货架期
Pub Date : 2024-07-14 DOI: 10.1016/j.afres.2024.100449
Joya Roy , Md. Nahidul Islam , Sabina Yasmin , Md. Sultan Mahomud

This work aimed to investigate the effect of edible coatings on shelf life and quality of tomato fruits. Aloe vera (AL) gel and tulsi leaf extract (TE), and a combination of aloe vera gel and tulsi extract (ALTE) coating, were applied to the surface of fresh tomatoes and stored at ∼26 °C and 75–82 % relative humidity for 25 days. Tomato fruits were evaluated periodically after the application of coating materials. Results showed that tomatoes coated with AL and TE maintained better quality than uncoated tomato fruits. Among various treatments, tomatoes coated with AL resulted in delayed weight loss (3.25 %), and ripening process, reduced disease incidence, and disease severity. It also showed that AL coated fruits had lower amount of lycopene (28.6 μg/g) and β carotene (11.42 μg/g), and a higher amount of phenolics (84.56 mg/100 gm) content. Disease incidence was significantly reduced by the coatings, with ALTE showing the lowest incidence (31 % at 25 days) compared to 100 % in the control. This novel edible coating technology can be applied industrially to enhance the quality and shelf life of mature tomato fruits.

这项工作旨在研究可食用涂层对番茄果实保质期和质量的影响。在新鲜番茄的表面涂上芦荟(AL)凝胶和图尔西叶提取物(TE),以及芦荟凝胶和图尔西提取物的组合(ALTE)涂层,并在∼26 °C和75-82 %的相对湿度下贮藏25天。涂抹涂层材料后,定期对番茄果实进行评估。结果表明,涂有 AL 和 TE 的番茄比未涂的番茄保持了更好的品质。在各种处理中,涂有 AL 的番茄延迟了重量损失(3.25%)和成熟过程,降低了病害发生率和病害严重程度。研究还表明,涂有 AL 的番茄果实番茄红素(28.6 μg/g)和β胡萝卜素(11.42 μg/g)含量较低,而酚类物质(84.56 mg/100 gm)含量较高。涂层能明显降低病害发生率,其中 ALTE 病害发生率最低(25 天时为 31%),而对照组为 100%。这种新型可食用涂层技术可应用于工业,以提高成熟番茄果实的质量和货架期。
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引用次数: 0
Valorization of Andean berries through whole-fruit powder development 通过开发全果粉实现安第斯浆果的价值化
Pub Date : 2024-07-14 DOI: 10.1016/j.afres.2024.100447
Manuela Gallón-Bedoya , Misael Cortés-Rodríguez , Jesús H Gil-González , José Luis Guil-Guerrero , Rodrigo Ortega-Toro

Modern consumer trends are directed towards the search for products that offer components with physiological activity and provide positive effects on health. This research is aimed to evaluate the effect of the formulation and process of the Andean berries mix powder of cape gooseberry-strawberry-blackberry (BP) using SD technology. A central composite design was used, considering the following independent variables (IV): inlet air temperature (IAT) (140–160 °C), outlet air temperature (OAT) (80–90 °C), atomization disk speed (ADS) (20,000–24,000 rpm), and maltodextrin (MD) (6–10 %), and the dependent variables (DV): moisture (M), hygroscopicity (Hy), solubility (S), wettability (We), particle size (D[3,2]), bulk density (ρbulk), antioxidant capacity (ABTS and DPPH methods), total phenolic compounds (TP), vitamin C, and yield (Y). Experimental optimization defined the formulation and process conditions: MD = 6.3 %, ADS = 24,000 rpm, IAT = 140.0 °C, OAT = 83.6 °C. The BP obtained the following quality attributes: M = 3.3 %, S = 62.9 %, Hy = 17.9 %, We = 8.7 min, ρbulk=0.396 g/mL, D [3,2] = 14.8 µm, ABTS = 1,478.3 mg TE/100 g db, DPPH = 1,000.6 mg TE/100 g db, TP = 860.4 mg GAE/100 g db, vitamin C = 18.1 mg AA/100 g db, Y = 62.5 %. The application of statistical tools contributes to developing Andean berries mix powder products maximizing antioxidant activity and physicochemical stability.

现代消费趋势是寻找具有生理活性并对健康有积极影响的产品。本研究旨在利用标度技术评估安第斯莓混合粉末(BP)的配方和加工过程的效果。采用了中心复合设计,考虑了以下自变量(IV):进气温度(IAT)(140-160 °C)、出气温度(OAT)(80-90 °C)、雾化盘转速(ADS)(20,000-24,000 rpm)和麦芽糊精(MD)(6-10 %),以及因变量(DV):水分 (M)、吸湿性 (Hy)、溶解性 (S)、润湿性 (We)、粒度 (D[3,2])、体积密度 (ρbulk)、抗氧化能力(ABTS 和 DPPH 法)、总酚类化合物 (TP)、维生素 C 和产量 (Y)。实验优化确定了配方和工艺条件:MD = 6.3%,ADS = 24,000 rpm,IAT = 140.0 °C,OAT = 83.6 °C。BP 获得了以下质量属性:M = 3.3 %,S = 62.9 %,Hy = 17.9 %,We = 8.7 min,ρbulk=0.396 g/mL,D [3,2] = 14.8 µm,ABTS = 1,478.3 mg TE/100 g db,DPPH = 1,000.6 mg TE/100 g db,TP = 860.4 mg GAE/100 g db,维生素 C = 18.1 mg AA/100 g db,Y = 62.5 %。统计工具的应用有助于开发抗氧化活性和理化稳定性最大化的安第斯浆果混合粉产品。
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引用次数: 0
Large-scale production of paraprobiotic soy milk in stirred tank bioreactor: A dual-step fermentation approach 在搅拌罐生物反应器中大规模生产副微生物豆奶:双步发酵法
Pub Date : 2024-07-14 DOI: 10.1016/j.afres.2024.100446
Chatchol Kongsinkaew , Kant Hongphankul , Thanakorn Soontornkitlert , Worawat Surarit , Manote Sutheerawattananonda , Phongphat Thitasirikul , Soisuda Pornpukdeewattana , Supenya Chittapun , Ketnarin Panpeang , Theppanya Charoenrat

This study investigates the feasibility of using soy milk as a substrate for cultivating Lactobacillus casei and its subsequent conversion into paraprobiotics. The research focuses on optimizing the fermentation process from shake flasks to bioreactor scale and assessing the antioxidant properties and proximate compositions of the resulting paraprobiotic soy milk beverage. The study highlights a dual-step large-scale fermentation process comprising an anaerobic fermentation step followed by a thermal inactivation step using the in situ temperature control system of the bioreactor. Results indicated that soy milk enriched with 10 g/L glucose and fermented at 37 °C for 24 h on flask scale provided optimal conditions for L. casei growth, achieving a bacterial count increase of 8.80 ± 0.11 log CFU/mL and a titratable acidity of 0.89 ± 0.03 %. These optimal conditions were employed for bioreactor scale fermentation, attaining a bacterial count of 10.02 ± 0.06 log CFU/mL and a titratable acidity of 1.07 ± 0.04 % at 24 h. The thermal inactivation step at 90 °C for 30 min successfully converted the probiotic soy milk into a paraprobiotic form, maintaining its antioxidant activity at 62.12 ± 2.58 % radical scavenging activity. The final paraprobiotic product met the Food and Drug Administration (FDA) specifications for titratable acidity, presenting a viable alternative for soy-based functional beverages.

本研究探讨了将豆奶作为培养干酪乳杆菌的基质并随后将其转化为副益生菌的可行性。研究重点是优化从摇瓶到生物反应器规模的发酵过程,并评估由此产生的副益生菌豆奶饮料的抗氧化特性和近似成分。研究强调了双步骤大规模发酵过程,包括厌氧发酵步骤和利用生物反应器的原位温度控制系统进行热灭活步骤。结果表明,富含 10 克/升葡萄糖的豆奶在 37 °C、24 小时的烧瓶规模下发酵,为干酪乳杆菌的生长提供了最佳条件,细菌数量增加了 8.80 ± 0.11 log CFU/mL,可滴定酸度为 0.89 ± 0.03 %。在这些最佳条件下进行生物反应器规模的发酵,24 小时后细菌数量达到 10.02 ± 0.06 log CFU/mL,可滴定酸度为 1.07 ± 0.04 %。90°C、30 分钟的热灭活步骤成功地将益生菌豆奶转化为副益生菌形式,其抗氧化活性保持在 62.12 ± 2.58 % 自由基清除活性。最终的副益生菌产品符合美国食品药品管理局(FDA)的可滴定酸度规范,为大豆功能饮料提供了一种可行的替代品。
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引用次数: 0
Effect of thermal processing on the degradation of pesticides in a banana jam partially formulated with banana peel flour 热加工对部分使用香蕉皮粉配制的香蕉果酱中农药降解的影响
Pub Date : 2024-07-09 DOI: 10.1016/j.afres.2024.100445
Magnólia Carneiro de Oliveira , John Kelvyn de Oliveira , Joselito Brilhante Silva , Luana Guabiraba Mendes , Felipe Sousa da Silva , Mairlane da Silva Alencar , Crisiana de Andrade Nobre , Mayra Garcia Maia Costa , Micael de Andrade Lima , Maria Aparecida Liberato Milhome

Bananas are one of the most widely consumed fruits in Brazil and across the world. However, the intensive use of pesticides in these and other crops can negatively impact human and animal health due to the possibility of pesticide residues persisting in derived products, even after industrial processing. Therefore, this study evaluated the effect of thermal processing on the degradation of the pesticides azoxystrobin, bifenthrin, difenoconazole, and simazine in samples of caramelized banana jam added of banana peel flour (4% w/w) in partial replacement of the fruit pulp. QuEChERS (quick, easy, cheap, effective, rugged, and safe) method and gas chromatography coupled to mass spectrometry (GC-MS) wa used to analyze the above compounds. The method was validated following the standard procedures of the European Commission and ANVISA. Samples of banana pulp were spiked with the pesticides at different concentrations (0.1, 0.5 and 1.0 mg.Kg-1) in order to observe their degradation following thermal processing. Degradation percentages ranged between 28 and 60 %, and these were potentially influenced by the physicochemical properties of each compound, as well as the characteristics of the food matrix. The thermal processing provided partial degradations of pesticide residues, some at levels below the MRLs (Maximum Residual Levels) established for bananas. Currently, there is no specific legislation in Brazil and in many other countries for controlling pesticides in processed foods, such as fruit jams. Therefore, this research highlights the need for the creation of new food laws by government agencies to this end to ensure the provision of safe food to the wide population.

香蕉是巴西乃至全世界消费量最大的水果之一。然而,在这些作物和其他作物中大量使用杀虫剂会对人类和动物的健康产生负面影响,因为即使经过工业加工,杀虫剂残留物仍有可能残留在衍生产品中。因此,本研究评估了热加工对添加香蕉皮粉(4% w/w)部分替代果肉的焦糖香蕉果酱样品中农药唑螨酯、联苯菊酯、苯醚甲环唑和西玛津降解的影响。采用 QuEChERS(快速、简便、廉价、有效、坚固和安全)方法和气相色谱-质谱联用(GC-MS)对上述化合物进行分析。该方法按照欧盟委员会和 ANVISA 的标准程序进行了验证。在香蕉果肉样品中添加了不同浓度(0.1、0.5 和 1.0 mg.Kg-1)的农药,以观察其在热加工后的降解情况。降解率介于 28% 和 60% 之间,可能受到每种化合物的物理化学特性以及食品基质特性的影响。热加工可使部分农药残留降解,其中一些含量低于为香蕉制定的最高残留限量(MRLs)。目前,巴西和许多其他国家都没有专门的法律来控制果酱等加工食品中的杀虫剂。因此,这项研究强调,政府机构有必要为此制定新的食品法,以确保为广大民众提供安全的食品。
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引用次数: 0
The impact of gamma irradiation on the quality of meat and poultry: A review on its immediate and storage effects 伽马辐照对肉类和家禽质量的影响:伽马辐照对肉类和家禽质量的影响:关于其直接影响和储存影响的综述
Pub Date : 2024-07-07 DOI: 10.1016/j.afres.2024.100444
Mohamad Shahrimi Hashim , Salma Mohamad Yusop , Irman Abdul Rahman

Gamma irradiation stands as a proven and efficient technology, elevating the safety, quality, and shelf life of meat and poultry. Its prowess lies in its capacity to eradicate harmful microbes and prolong shelf life without inducing radioactivity. The utilisation of gamma irradiation emerges as an energy-efficient, safe, and environmentally friendly method. Irrespective of dosage, the application of gamma irradiation significantly reduces microorganisms and moisture content, while concurrently enhancing TVBN, PV, TBARS, colour a*, and fat content. However, it caused no significant impact on pH, sensory properties, colour L* and b*, protein, and ash content. During storage, gamma-irradiated meat and poultry experience a decline in pH, sensory properties, colour a*, moisture, protein, and ash content. On the other hand, microbial load, shelf life, TVBN, PV, TBARS, and fat content increase, while colour L* and b* remain unchanged. Precise dosage selection is paramount to effectively manage these changes. This comprehensive review explores both current advancements and potential developments within the gamma-irradiated meat and poultry. It is imperative not only to scrutinize the effects of gamma irradiation utilisation across various muscle-based systems but also to examine its efficacy with the use of diverse packaging methods, alternative preservation techniques, functional food ingredients in food systems, and consumer acceptance studies across different regions. Such investigations will bolster understanding and application of gamma irradiation, ensuring its efficacy and acceptance within the meat and poultry industry.

伽马辐照是一项成熟、高效的技术,可提高肉类和家禽的安全性、质量和保质期。它的优势在于能够消灭有害微生物,延长保质期,而不会产生放射性。伽马辐照是一种节能、安全和环保的方法。无论用量多少,伽马辐照都能显著减少微生物和水分含量,同时提高 TVBN、PV、TBARS、色素 a* 和脂肪含量。然而,伽马辐照对 pH 值、感官特性、色泽 L* 和 b*、蛋白质和灰分含量没有明显影响。在储存过程中,经伽马射线照射的肉类和家禽的 pH 值、感官特性、色泽 a*、水分、蛋白质和灰分含量都会下降。另一方面,微生物量、保质期、TVBN、PV、TBARS 和脂肪含量会增加,而颜色 L* 和 b* 则保持不变。要有效控制这些变化,精确的剂量选择至关重要。本综述探讨了伽马辐照肉类和家禽的当前进展和潜在发展。当务之急不仅是要仔细研究伽马辐照在各种肌肉系统中的应用效果,还要研究其在不同包装方法、替代保存技术、食品系统中的功能性食品配料以及不同地区消费者接受度研究中的应用效果。这些研究将加深人们对伽马辐照的理解和应用,确保其在肉类和家禽行业中的有效性和认可度。
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引用次数: 0
DART-HRMS reveals metabolic changes of whey through microparticulation and fermentations DART-HRMS 通过微颗粒化和发酵揭示乳清的代谢变化
Pub Date : 2024-07-06 DOI: 10.1016/j.afres.2024.100443
Carmela Zacometti , Sara Khazzar , Andrea Massaro , Alessandra Tata , Giorgia Riuzzi , Roberto Piro , Enrico Novelli , Severino Segato , Stefania Balzan

Despite native whey (hereafter termed WHEY) being a watery by-product rich in high-value nutritional components, it poses environmental concerns. Recently, the techniques of thermal-mechanical microparticulation to produce microparticulated whey (MPW) and a further process to ferment MPW (FMPW) were suggested, in order to recycle whey into a protein concentrated soft dairy cream that acquires also fat mimicking functionality. Aiming at monitoring the effects of this recycling process on the products’ metabolic profiles, samples (n = 8) of WHEY, MPW and two sub-types of FMPW were analysed by combining direct analysis in real time coupled with high resolution mass spectrometry (DART-HRMS). The most informative ions were used to build a partial least squared discriminant analysis (PLS-DA) model to assess the biochemical compounds that characterize and differentiate WHEY from MPW and FMPW. The DART-HRMS fingerprints acquired in negative mode accurately differentiated samples throughout the steps of microparticulation and fermentation. Post-fermentation, greater relative abundances of lactic acid, glucosamine and histidyl-aspartic acid were detected in both subtypes of FMPW, which were moderately differentiated by DART-HRMS. Moreover, while WHEY and MPW contained high relative abundances of hydroxyglutaric and malic acids, the fermented derivates (FMPW) were characterised by elevated levels of volatile compounds (tetradecanal, hexadecene and tetradecene) and fermentative end-products (diethyltartrate and histidyl-aspartic acid). DART-HRMS successfully captured a pool of informative biomarkers useful to understand the chemical and metabolomic changes occurring in microparticulation and fermentation processes used to recycle whey. This analytical technique is an operative supporting step needed for comprehensive assessment of the nutritional and organoleptic properties of the proposed whey-based soft cream dairy food.

尽管原生乳清(以下简称 "乳清")是一种含水副产品,富含高价值的营养成分,但它也带来了环境问题。最近,有人建议采用热机械微颗粒化技术生产微颗粒化乳清(MPW),并进一步采用发酵工艺生产微颗粒化乳清(FMPW),以便将乳清回收利用,制成蛋白质浓缩软乳脂,同时获得脂肪模拟功能。为了监测这种回收工艺对产品代谢特征的影响,我们结合实时直接分析和高分辨率质谱法(DART-HRMS)对乳清、MPW 和两种亚型 FMPW 样品(n = 8)进行了分析。信息量最大的离子被用于建立部分最小平方判别分析(PLS-DA)模型,以评估表征和区分 WHEY 与 MPW 和 FMPW 的生化化合物。在阴性模式下获得的 DART-HRMS 指纹能准确地区分样品的微颗粒化和发酵过程。发酵后,在两个亚型的 FMPW 中都检测到了较多的乳酸、葡萄糖胺和组氨酰-天冬氨酸,DART-HRMS 对其进行了中度区分。此外,虽然 WHEY 和 MPW 含有较高相对丰度的羟基戊二酸和苹果酸,但发酵衍生物(FMPW)的特点是挥发性化合物(十四醛、十六烯和十四烯)和发酵终产物(酒石酸二乙酯和组天冬氨酸)含量较高。DART-HRMS 成功捕获了一批信息丰富的生物标记物,有助于了解用于回收乳清的微粉碎和发酵过程中发生的化学和代谢组学变化。这项分析技术是全面评估拟议的以乳清为基础的软奶油乳制品的营养和感官特性所需的操作性辅助步骤。
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引用次数: 0
Optimization of germinated cowpea flour ratios and extrusion conditions on the nutritional composition and sensory quality of Ethiopian emmer wheat-based extruded porridge 优化发芽豇豆粉比例和挤压条件对埃塞俄比亚小麦挤压粥营养成分和感官质量的影响
Pub Date : 2024-07-01 DOI: 10.1016/j.afres.2024.100442
Desye Alemu Teferi , Neela Satheesh , Messenbet Geremew Kassa

Childhood malnutrition is a major problem in developing countries, particularly in Ethiopia. This study aimed to create nutritious emmer wheat and cowpea extruded porridge with optimal macronutrient levels and sensory quality. Utilizing Design-Expert software, the effects of cowpea blending ratios (10–25 %), barrel temperatures (80–120 °C), and feed moisture content (22–26 %) were analyzed using a three-factor, three-level Box-Behnken design. Significant impacts on nutritional characteristics were observed, leading to the identification of optimal extrusion conditions (cowpea blend: 20.17 %, moisture content: 25.91 %, barrel temperature: 117.20 °C). Blending emmer wheat with cowpea flour significantly enhanced the porridge's color, taste, and aroma. The highest color intensity (4.36 %) was at 10 % cowpea and 22 % moisture processed at 100 °C. The best taste (4.41 %) was with 25 % cowpea and 22 % moisture at 100 °C, while the highest aroma was in blends with 10 % and 25 % cowpea at 100 °C. The control had the best mouthfeel (4.36 %), but a blend with 25 % cowpea, 24 % moisture, and 80 °C barrel temperature had the highest overall acceptability (4.31 %). The study highlighted the potential of these protein-rich porridge products to address protein-energy malnutrition, particularly in Ethiopia, by introducing awareness about the unique nutritional composition of Ethiopian emmer wheat and underutilized cowpea, thus creating a new market for nutritious food options.

儿童营养不良是发展中国家,尤其是埃塞俄比亚的一个主要问题。本研究旨在制作营养丰富的小麦和豇豆挤压粥,以获得最佳的常量营养素水平和感官质量。利用 Design-Expert 软件,采用三因素、三水平 Box-Behnken 设计分析了豇豆混合比例(10-25%)、料筒温度(80-120 °C)和饲料水分含量(22-26%)的影响。结果表明,豇豆混合比(20.17%)、饲料含水量(25.91%)、料筒温度(80-120 °C)和饲料含水量(22-26%)对营养特性有显著影响,从而确定了最佳挤压条件:25.91 %,机筒温度:117.20 °C):117.20 °C).将小麦与豇豆粉混合后,粥的色泽、口感和香气都有明显改善。豇豆含量为 10%、水分为 22%、温度为 100 °C 时,色度最高(4.36%)。口感最好(4.41%)的是在 100 °C 下添加 25 % 的豇豆和 22 % 的水分,而香气最高的是在 100 °C 下添加 10 % 和 25 % 的豇豆的混合物。对照组的口感最好(4.36 %),但豇豆含量为 25 %、水分为 24 %、桶温为 80 °C的混合物的总体可接受性最高(4.31 %)。这项研究强调了这些富含蛋白质的粥类产品在解决蛋白质-能量营养不良方面的潜力,特别是在埃塞俄比亚,通过介绍埃塞俄比亚埃默小麦和未充分利用的豇豆的独特营养成分,从而为营养食品选择创造一个新的市场。
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引用次数: 0
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Applied Food Research
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