Species of Castanopsis (Ca.) and Cyclobalanopsis (Cy.) (Fagaceae) serve as important carriers of multifunctional forest foods and bioactive resources; however, their seeds remain underutilized in food development. To address this gap and the potential impacts of global climate change on Quercus resources, this study systematically evaluated the morphological, nutritional, and functional traits of seeds from five Ca. species (Ca. kawakamii, Ca. jucunda, Ca. hystrix, Ca. fargesii, Ca. lamontii) and three Cy. species (Cy. pachyloma, Cy. fleuryi, Cy. glauca) in southern China. Results showed that Cy. pachyloma seeds (13.75 ± 2.47 g) were the largest, while Ca. fargesii (0.42 ± 0.12 g) and Ca. hystrix (0.54 ± 0.13 g) produced relatively small seeds. The main carbohydrates were starch (29.03 ± 0.58 % – 38.85 ± 0.29 %) and reducing sugars (31.22 ± 0.26 % – 41.34 ± 0.61 %). Fatty acids were dominated by linoleic, palmitic, and oleic acids, with unsaturated fatty acids accounting for 65.785 – 79.264 %. Tocopherols were mainly α- and γ-isomers, with Cy. glauca exhibiting the highest α-tocopherol content (77.605 mg/Kg). A comprehensive quality model identified Cy. pachyloma (M = 2.69) and Cy. fleuryi (M = 2.11) as top-ranking species. Overall, the study reveals interspecific differences in nutritional and functional components of Ca. and Cy. seeds, providing a scientific foundation for their utilization as resilient, high-value forest food resources.
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