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Pancratistatin Inhibits the Growth of Colorectal Cancer Cells by Inducing Apoptosis, Autophagy, and G2/M Cell Cycle Arrest. Pancratistatin 通过诱导细胞凋亡、自噬和 G2/M 细胞周期停滞来抑制结直肠癌细胞的生长
Pub Date : 2019-08-12 DOI: 10.12659/MSM.916116

BACKGROUND Worldwide, colorectal cancer is ranked as the third most prevalent cancer. The natural compound, pancratistatin, extracted from the spider lily, has previously been shown to target apoptosis in cancer cells lines. This study aimed to investigate the effects of pancratistatin in human colorectal cancer cells in vitro. MATERIAL AND METHODS Human colorectal cancer cell lines, including HTC-15 cells, were compared with a normal human colonic fibroblast cell line, CDD-18Co. Cells were treated with increasing doses of pancratistatin. The MTT assay was used to assess cell viability. Fluorescence microscopy using DAPI and Annexin-V/propidium iodide (PI) was used to detect cell apoptosis. Cell autophagy was detected by electron microscopy. Cell migration was evaluated using a wound healing assay, and Western blot determined the expression levels of cell cycle proteins. RESULTS Pancratistatin inhibited the growth of the colorectal cancer cells with an IC₅₀ ranging from 15-25 µM, but had a limited effect in normal CCD-18Co cells, with an IC₅₀ of >100 µM. Pancratistatin reduced HCT-15 cell migration. Growth inhibition due to pancratistatin was associated with morphological changes of HCT-15 cells and included autophagy and apoptosis, and increased expression the autophagic proteins, LC3II, beclin-1, and Bax. Pancratistatin induced arrest of HCT-15 cells at G2/M of the cell cycle and inhibited phosphorylation of cdc2/cyclin-dependent kinase 1 (CDK1) and Cdc25c and the expression of cyclin B1. CONCLUSIONS Pancratistatin inhibited the growth of colorectal cancer cells in vitro by inducing apoptosis, autophagy, and G2/M cell cycle arrest.

背景 在全球范围内,结肠直肠癌是发病率排名第三的癌症。从蜘蛛百合中提取的天然化合物 pancratistatin 以前曾被证明能针对癌细胞株的凋亡。本研究旨在体外研究 pancratistatin 对人类结直肠癌细胞的影响。材料与方法 将包括 HTC-15 细胞在内的人类结直肠癌细胞系与正常人类结肠成纤维细胞系 CDD-18Co 进行比较。细胞接受剂量不断增加的泛普瑞司他丁处理。MTT 试验用于评估细胞活力。使用 DAPI 和 Annexin-V/propidium iodide (PI) 荧光显微镜检测细胞凋亡。电子显微镜检测细胞自噬。使用伤口愈合试验评估细胞迁移,并通过 Western 印迹检测细胞周期蛋白的表达水平。结果 Pancratistatin能抑制结直肠癌细胞的生长,IC₅₀为15-25 µM,但对正常CCD-18Co细胞的作用有限,IC₅₀大于100 µM。Pancratistatin 可减少 HCT-15 细胞的迁移。Pancratistatin导致的生长抑制与HCT-15细胞的形态变化有关,包括自噬和凋亡,以及自噬蛋白LC3II、beclin-1和Bax的表达增加。Pancratistatin 可诱导 HCT-15 细胞停滞在细胞周期的 G2/M,并抑制 cdc2/细胞周期蛋白依赖性激酶 1(CDK1)和 Cdc25c 的磷酸化以及细胞周期蛋白 B1 的表达。结论 Pancratistatin通过诱导细胞凋亡、自噬和G2/M细胞周期停滞来抑制体外结直肠癌细胞的生长。
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引用次数: 0
California Dreams or Colonial Nightmares 加州梦或殖民噩梦
Pub Date : 2018-01-01 DOI: 10.17688/NTR.V30I1.1391
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引用次数: 0
Monasticism and the Catholic Charismatic Renewal Movement 修道主义和天主教灵恩复兴运动
Pub Date : 2017-03-16 DOI: 10.17688/NTR.V29I2.1275
Christian monasticism is a gift of the Holy Spirit to the Church. Many centuries ago the Holy Spirit guided women and men to devote their lives to follow Jesus Christ closely and serve all the people. Since then, monasticism has greatly contributed in keeping the flame of the Holy Spirit alive and bringing renewal in the Church. The Catholic Charismatic Renewal Movement, which is a comparatively young movement within the Church, is also a Spirit-centered movement. Charismatics seek the renewal of their spiritual lives through the power of the Holy Spirit. In this paper, we shall examine the main features of both monasticism and the Charismatic Renewal Movement to explore the spiritual richness in these traditions. Further, this paper will also examine if these movements could help the contemporary people to discover the beauty of their faith and find meaning in it. The sources for our study will be the Church documents, literature on monasticism, Catholic Charismatic Renewal Movement and opinions of some experts in these fields.
基督教修道是圣灵给教会的礼物。几个世纪前,圣灵引导妇女和男子奉献他们的一生,密切追随耶稣基督,为所有的人民服务。从那时起,修道在保持圣灵之火的活力和教会的复兴方面做出了巨大贡献。天主教魅力复兴运动是教会内部一个相对年轻的运动,也是一个以精神为中心的运动。魅力主义者寻求通过圣灵的力量来更新他们的精神生活。在本文中,我们将考察修道主义和魅力复兴运动的主要特征,以探索这些传统中的精神丰富性。此外,本文还将考察这些运动是否能帮助当代人发现他们信仰的美并在其中找到意义。我们的研究来源将是教会文献、修道院文学、天主教魅力复兴运动以及这些领域的一些专家的意见。
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引用次数: 0
Christian Faith in a Secular Age 世俗时代的基督教信仰
Pub Date : 2017-03-16 DOI: 10.17688/NTR.V29I2.1341
This article offers an analysis of secularization from within the Christian tradition, with an emphasis on the challenge that the moral and metaphysical dissensus created by secularization causes, not only for a Christian conception of the world, but even for the very possibility of establishing a persuasive case for Christianity in the midst of the social and ideological fragmentation of contemporary society. Beginning with Charles Taylor’s analysis of secularism, it will consider several perspectives on the relationship between modernity, secularism, and the breakdown of moral consensus in the modern world. Finally, it will turn to the question of how Christians should engage with integrity with the secular context of modernity.
本文从基督教传统的角度对世俗化进行了分析,强调世俗化所造成的道德和形而上学的分歧所带来的挑战,不仅是对基督教世界观的挑战,但即使是在当代社会的社会和意识形态分裂中为基督教建立一个有说服力的案例的可能性。从查尔斯·泰勒对世俗主义的分析开始,我们将从几个角度来看待现代性、世俗主义和现代世界道德共识破裂之间的关系。最后,它将转向基督徒应该如何在现代性的世俗背景下保持完整的问题。
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引用次数: 0
Witnessing in the Field Hospital of the Church 在教会野战医院作见证
Pub Date : 2017-03-16 DOI: 10.17688/NTR.V29I2.1330
In this paper, I work to correlate three aspects of a specific event (a community gathered around a woman considering suicide) with the salvific promise of the life, death, and resurrection of Jesus Christ in order to elucidate the potential power of narrative in preaching to name and call forth a real and durable hope in our times.  To do this, I base my method on David Tracy’s analogical imagination, and develop some of the contrasts and connecting points of contemporary trauma theory regarding memory and experience with Johann Baptist Metz’s notion of memory as it relates to the eruption of apocalyptic hope in our times.  I also reflect on the implications of an often neglected Holy Saturday faith for preaching. My hope is that this work will be a resource and a caution to preachers as they work to articulate the vision of hope erupting into division and hopelessness in concrete parish, social, and global contexts today.
在这篇论文中,我致力于将一个特定事件(一个聚集在一名考虑自杀的妇女周围的社区)的三个方面与耶稣基督的生、死和复活的救赎承诺联系起来,以阐明叙事在我们这个时代命名和唤起真正持久希望的潜在力量。为了做到这一点,我的方法基于大卫·特雷西的类比想象,并发展了当代创伤理论中关于记忆和经验的一些对比和联系点,与约翰·巴ptist Metz的记忆概念相联系,因为它与我们时代世界末日希望的爆发有关。我还反思了一个经常被忽视的神圣星期六信仰对传教的影响。我希望这项工作将成为传教士的一种资源和警告,因为他们正在努力阐明希望在当今具体的教区、社会和全球背景下爆发为分裂和绝望的愿景。
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引用次数: 0
Lessons of Martyrdom on Contemporary Immigration 殉难对当代移民的启示
Pub Date : 2016-09-29 DOI: 10.17688/NTR.V29I1.1251
Confucianism greatly influenced the dynamics of the Korean monarchy and family hierarchical system in the 19th century when Catholicism was introduced by the educated yangban class. Catholic teachings so impressed Koreans that their loyalties toward the king were challenged by their new love for God, which went against societal norms based on filial piety. Eventually their disloyalty to the king led to thousands of martyrs. While some scholars may wonder at their courage, others felt their self-sacrifice might have been easier than other non-Confucian based cultures, as they had merely transferred their loyalty from a king to God. Thus, filial piety never disappeared with the appearance of the Catholic faith, as martyrs continued unto death while being loyal in their minds. Today, however, Korean American Catholics struggle with this Confucian heritage embedded in the Catholic faith, especially within the immigration context. The expectations of filial piety from the next generation causes friction because they lack sufficient cultural and historical awareness. Thus, this article looks at the Korean martyrs for re-envisioning traditional values especially when immigration radically transforms the ethnic faith narrative.
19世纪,当天主教被受过良好教育的养班阶层引入朝鲜时,儒家思想极大地影响了朝鲜君主制和家庭等级制度的动态。天主教教义给韩国人留下了深刻的印象,以至于他们对国王的忠诚受到了对上帝的新爱的挑战,这违背了以孝道为基础的社会规范。最终,他们对国王的不忠导致了成千上万的殉道者。虽然一些学者可能会对他们的勇气感到惊讶,但其他人认为他们的自我牺牲可能比其他非儒家文化更容易,因为他们只是将他们的忠诚从国王转移到上帝。因此,孝道从来没有随着天主教信仰的出现而消失,殉道者在他们的思想中保持忠诚,直到死亡。然而,今天的韩裔美国天主教徒却在与植根于天主教信仰中的儒家传统作斗争,尤其是在移民背景下。由于缺乏足够的文化和历史意识,下一代对孝道的期望引起了摩擦。因此,本文着眼于韩国烈士重新设想传统价值观,特别是当移民从根本上改变了民族信仰叙事时。
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引用次数: 0
In the Aftermath of a Synod: The Sacramental Vision of the Universe and a Case for the Development of Doctrine 主教会议的后果:圣礼对宇宙的看法和教义发展的一个案例
Pub Date : 2016-09-29 DOI: 10.17688/NTR.V29I1.1219
In the aftermath of the Synod on the Family, the question of the development of doctrine has gained renewed prominence. This article considers the question in light of the principle of sacramentality. If all of creation is infused with potential to mediate the divine, then this applies to reason, experience, and derived processes of communication, interpretation, and development. Understood within this sacramental framework, the development of doctrine is not a matter of compromising eternal truths so as to meet the demands of secular modernity so much as a grace-filled sharing of wisdom between the Word of God and the People of God, the Mystical Body of Christ in a given epoch. The article contrasts Alfred Loisy’s intrinsicist model of development wherein reason is infused with grace with the dualistic assumptions of Neo-Scholasticism. It explores Pope St. John XXIII’s distinction between the changeable manner in which doctrine is articulated and understood and its unchanging substance, that is, the eternal truths. Finally, in the dialogical disposition of Vatican II in relation to the modern world, the article detects an intrinsicist perspective on the development of doctrine, offering hope for the possibility of more complete expressions of timeless truths.
在关于家庭的世界主教会议之后,教义发展的问题重新获得突出地位。本文考虑的问题,根据原则的圣礼。如果所有的创造物都注入了调解神性的潜力,那么这适用于理性、经验和衍生的交流、解释和发展过程。在这个圣事的框架内理解,教义的发展不是为了满足世俗现代性的要求而妥协永恒真理的问题,而是在特定时代天主圣言与天主子民、基督的奥体之间充满恩宠的智慧分享。本文将卢瓦西的内在主义发展模式与新经院哲学的二元假设进行了对比。它探讨了教皇圣约翰二十三世的区别,在多变的方式,其中学说是明确和理解和其不变的实质,即永恒的真理。最后,在梵蒂冈第二次会议与现代世界的对话中,本文发现了教义发展的内在主义观点,为更完整地表达永恒真理的可能性提供了希望。
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引用次数: 0
Tradition and Church Reform: Perspectives on Catholic Moral Teaching 传统与教会改革:天主教道德教育的视角
Pub Date : 2016-09-29 DOI: 10.17688/NTR.V29I1.1255
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引用次数: 1
The Theme of Communion in Laudato Si' and Its Implications for Ecclesiology 《愿祢受赞颂》的共融主题及其对教会学的启示
Pub Date : 2016-09-29 DOI: 10.17688/NTR.V29I1.1228
This article explores the theme of communion in the encyclical Laudato Si’ and in other writings and addresses of Pope Francis. It depicts the connections between Francis’s appeal to this theme in his ecological theology and his employment of it in his descriptions of the church. It describes the way in which communion is foundational for Francis’s theological anthropology, ecological theology, and ecclesiology. The author also adduces recent critiques of communio ecclesiology, especially that of Australian theologian Neil Ormerod. He explores whether the ecclesiology of Pope Francis meets the concerns of these critiques. The article concludes with an affirmation of the significance of communion for contemporary ecclesiology, especially in light of two current pastoral concerns.
这篇文章探讨了共融的主题,在通谕“赞美你”和其他著作和演讲教皇弗朗西斯。它描绘了方济各在他的生态神学中对这个主题的呼吁,以及他在描述教会时对这个主题的运用之间的联系。它描述了共融是方济各的神学人类学、生态神学和教会学的基础。作者还引用了最近对社会教会学的批评,特别是澳大利亚神学家尼尔·奥默罗德的批评。他探讨了教宗方济各的教会学是否符合这些批评的关切。文章最后肯定了共融对当代教会学的重要性,特别是考虑到当前的两个牧灵问题。
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引用次数: 0
Incarnating the Feminine Genius in the Contemporary Catholic Church 当代天主教会中女性天才的体现
Pub Date : 2016-09-29 DOI: 10.17688/NTR.V29I1.1260
As Western society has seemingly become apathetic about faith and religion [1] , many within the Catholic Church have simultaneously become concerned with issues of relevance. The call from Pope Benedict XVI and Pope Francis has been to examine the fruits of the Second Vatican Council, with a view to evangelisation, emphasising the role of the Church in modern society. Responses to this call on a pastoral level, however, often fail to recognise the specific needs of women in society and in the Church today. The goal of this article is to examine practical movements in the contemporary Church that are instigated by women, addressing the distinct needs of women in our communities in terms of vocation, spiritual motherhood and the ‘feminine genius’ (Edith Stein), with receptivity and empathy for others, including the disenfranchised and excluded. Is the Church on a local level utilising women’s ‘care thinking’ [2] in the practical outpourings of its faith and charity? In answer to this question, this article discusses the emergence of grassroots movements that exemplify the incarnation of the feminine genius in the contemporary Catholic Church. [1] Roy Williams, Post God Nation? How religion fell off the radar in Australia – and what might be done to get it back on, (Sydney, Australia: ABC Books, 2015) 28. [2] Carol Gilligan,  In a different voice: Psychological theory and women's development , (Cambridge, MA: Harvard University Press, 1982.1983). Leonie Westenberg Associate Lecturer, Theology University of Notre Dame Australia, Sydney campus 104 Broadway (P.O. Box 944) Broadway NSW 2007 E: leonie.westenberg@nd.edu.au T: (02) 8204 4175 (School) Degrees: Masters of Theology, Charles Sturt University, Australia. Postgraduate Diploma Theological Studies, Flinders University, Australia. Postgraduate Certificate Theological Studies, Flinders University, Australia. Diploma Teaching, Edith Cowan University, Australia. Biographical information: Leonie Westenberg is an Associate Lecturer in Theology at Notre Dame University Australia, Sydney campus. She lectures in theology in the Logos programme, the university’s core curriculum. She has been the author of and contributor to several books on education and has written for The Conversation on ‘The paradox of work over the summer season’, and “Blessed are the Hunger Games? Katniss Everdeen lives the Beatitudes’. She has presented papers at the Society for the Study of Theology April 2015 conference at the University of Nottingham on ‘Thinking the Church Today: What are the implications of the Second Vatican Council, fifty years, on for women, in light of the discussion of Mary in Lumen Gentium ?’, and at the 2016 Marian Conference at Notre Dame University Australia on Literary Apologetics. Email address: leonie.westenberg@nd.edu.au
随着西方社会似乎对信仰和宗教变得冷漠,天主教会内部的许多人同时开始关注相关问题。教皇本笃十六世(Pope Benedict XVI)和教皇方济各(Pope Francis)的呼吁是审视梵蒂冈第二届大公会议的成果,着眼于福音化,强调教会在现代社会中的作用。然而,在牧灵层面上对这一呼吁的回应,往往未能认识到妇女在当今社会和教会中的具体需求。本文的目的是研究当代教会中由女性发起的实际运动,解决我们社区中女性在职业、精神母性和“女性天才”(伊迪丝·斯坦)方面的独特需求,并对他人(包括被剥夺公民权和被排斥的人)的接受和同情。在地方层面上,教会是否在其信仰和慈善的实际产出中利用了女性的“关怀思维”?为了回答这个问题,本文讨论了草根运动的出现,这些运动体现了当代天主教会中女性天才的化身。罗伊·威廉姆斯,后上帝国度?《宗教在澳大利亚是如何被忽视的——以及如何让它重新受到关注》,(澳大利亚悉尼:ABC Books, 2015)。[10]卡罗尔·吉利根:《以不同的声音:心理学理论与女性发展》(马萨诸塞州剑桥:哈佛大学出版社,1982 - 1983)。列奥尼·韦斯滕伯格,澳大利亚圣母大学神学大学副讲师,悉尼校区104 Broadway (P.O. Box 944) Broadway NSW 2007 E: leonie.westenberg@nd.edu.au T:(02) 8204 4175(学院)学位:神学硕士,Charles Sturt大学,澳大利亚。研究生文凭神学研究,弗林德斯大学,澳大利亚研究生神学证书,弗林德斯大学,澳大利亚。澳大利亚伊迪丝考恩大学文凭教学。简介:莱昂尼·威斯特伯格是澳大利亚圣母大学悉尼校区的神学副讲师。她在大学的核心课程Logos课程中讲授神学。她是几本教育书籍的作者和撰稿人,并为《对话》杂志撰写了《夏季工作的悖论》和《饥饿游戏有福了?》凯特尼斯·伊夫狄恩过着幸福的生活。她曾在2015年4月诺丁汉大学神学研究学会会议上发表论文,题为“思考今天的教会:梵蒂冈第二届大公会议,五十年来,对女性的影响是什么?”,并在2016年圣母大学澳大利亚玛丽安文学护教学会议上发表论文。邮箱:leonie.westenberg@nd.edu.au
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