Pub Date : 2000-06-01DOI: 10.1051/ANALUSIS:2000121
N. Schulmann-Choron, P. Chevallier, J. Ceaurriz, C. Souleau
Selenium, an ubiquitous essential trace-element, is known to be particularly difficult to measure especially in brain. First, it is a non metal, next, at very low concentration (below ppm); at last, the brain matter, very rich in lipids, make the diges- tion specially uneasy. Using synchrotron radiation induced X-ray fluorescence analysis (SXRF), selenium in rat brain was mea- sured equal to CSe = 124 ± 5.4 ppb with a MDL (minimum detection limit) of 20 ppb. The obtained values should be used as a first step to study human brain on extremely small and specific locations. With the development of aging pathologies (such as Alzheimer's or Parkinson's disease...), it matters to get informations about selenium known as an anti-aging element. The obtai ned values from rat, near of man's, may also highlight it as a potential animal model system for studying selenium in human brain.
{"title":"Determination of selenium in rat brain by synchrotron radiation X-ray fluorescence","authors":"N. Schulmann-Choron, P. Chevallier, J. Ceaurriz, C. Souleau","doi":"10.1051/ANALUSIS:2000121","DOIUrl":"https://doi.org/10.1051/ANALUSIS:2000121","url":null,"abstract":"Selenium, an ubiquitous essential trace-element, is known to be particularly difficult to measure especially in brain. First, it is a non metal, next, at very low concentration (below ppm); at last, the brain matter, very rich in lipids, make the diges- tion specially uneasy. Using synchrotron radiation induced X-ray fluorescence analysis (SXRF), selenium in rat brain was mea- sured equal to CSe = 124 ± 5.4 ppb with a MDL (minimum detection limit) of 20 ppb. The obtained values should be used as a first step to study human brain on extremely small and specific locations. With the development of aging pathologies (such as Alzheimer's or Parkinson's disease...), it matters to get informations about selenium known as an anti-aging element. The obtai ned values from rat, near of man's, may also highlight it as a potential animal model system for studying selenium in human brain.","PeriodicalId":8221,"journal":{"name":"Analusis","volume":"69 1","pages":"316-323"},"PeriodicalIF":0.0,"publicationDate":"2000-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86961341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2000-06-01DOI: 10.1051/ANALUSIS:2000280263
S. Varray, J. Aubagnac, F. Lamaty, R. Lazaro, J. Martinez, C. Enjalbal
Poly(ethyleneglycol) (PEG) substituted by various organic moities were analyzed by electrospray ionization (ESI) mass spectrometry in order to determine the best experimental protocol allowing reproducible, sensitive and informative spectra in the search of completely automated high throughput identification of libraries generated by the liquid-phase methodology. The nature of the eluent and of the PEG derivatization as well as the value of the sampling cone voltage were investigated by comparing the recorded oligomer distributions.
{"title":"Poly(ethyleneglycol) in electrospray ionization (ESI) mass spectrometry","authors":"S. Varray, J. Aubagnac, F. Lamaty, R. Lazaro, J. Martinez, C. Enjalbal","doi":"10.1051/ANALUSIS:2000280263","DOIUrl":"https://doi.org/10.1051/ANALUSIS:2000280263","url":null,"abstract":"Poly(ethyleneglycol) (PEG) substituted by various organic moities were analyzed by electrospray ionization (ESI) mass spectrometry in order to determine the best experimental protocol allowing reproducible, sensitive and informative spectra in the search of completely automated high throughput identification of libraries generated by the liquid-phase methodology. The nature of the eluent and of the PEG derivatization as well as the value of the sampling cone voltage were investigated by comparing the recorded oligomer distributions.","PeriodicalId":8221,"journal":{"name":"Analusis","volume":"79 1","pages":"263-268"},"PeriodicalIF":0.0,"publicationDate":"2000-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74538829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2000-06-01DOI: 10.1051/ANALUSIS:2000119
R. Vivier, L. Muhr, H. Muhr, E. Plasari
This paper deals with a procedure to determine the residual sodium content in silver oxide-metal oxide (intermediary product) and silver-metal oxide composites. In addition, an equilibrium curve showing the evolution of sodium content in the intermediary product as a function of sodium concentration in the washing liquid is given. So the residual sodium content in the solid may be subsequently deduced by measurement of the sodium concentration in the corresponding washing liquid. The influence of both silver and sodium contents on the procedure is also investigated.
{"title":"Determination of residual sodium content in silver and silver oxide composite materials","authors":"R. Vivier, L. Muhr, H. Muhr, E. Plasari","doi":"10.1051/ANALUSIS:2000119","DOIUrl":"https://doi.org/10.1051/ANALUSIS:2000119","url":null,"abstract":"This paper deals with a procedure to determine the residual sodium content in silver oxide-metal oxide (intermediary product) and silver-metal oxide composites. In addition, an equilibrium curve showing the evolution of sodium content in the intermediary product as a function of sodium concentration in the washing liquid is given. So the residual sodium content in the solid may be subsequently deduced by measurement of the sodium concentration in the corresponding washing liquid. The influence of both silver and sodium contents on the procedure is also investigated.","PeriodicalId":8221,"journal":{"name":"Analusis","volume":"4 1","pages":"302-307"},"PeriodicalIF":0.0,"publicationDate":"2000-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76344795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2000-06-01DOI: 10.1051/ANALUSIS:2000280351
C. Ferrarello, M. Campa, H. G. Infante, M. L. F. Sánchez, A. Sanz-Medel
The analytical performance of the Fast - Protein Liquid Chromatography (FPLC) (on a Mono Q HR 5/5 column) and the conventional AnionExchange Chromatography (on a Protein Pak DEAE 5PW column) to realise rabbit liver metallothioneins separations and Cd speciation has been investigated and the results are compared. The Mono Q column was found to provide higher resolution and reproducibility in shorter separation times than Protein Pak DEAE 5PW, a conventional anion-exchange column. Thus, FPLC is proposed as a very useful tool for the study of the polymorphism of metallothioneins.
研究了快速蛋白液相色谱法(Mono Q HR 5/5色谱柱)和传统阴离子交换色谱法(Protein Pak DEAE 5PW色谱柱)分离兔肝脏金属硫蛋白和Cd形态的分析性能，并对结果进行了比较。与传统的阴离子交换柱Protein Pak DEAE 5PW相比，Mono Q柱在更短的分离时间内具有更高的分辨率和重现性。因此，FPLC被认为是研究金属硫蛋白多态性的一个非常有用的工具。
{"title":"Improved separation of rabbit liver metallothioneins by FPLC-ICP-MS: a comparison with the conventional anion-exchange chromatography","authors":"C. Ferrarello, M. Campa, H. G. Infante, M. L. F. Sánchez, A. Sanz-Medel","doi":"10.1051/ANALUSIS:2000280351","DOIUrl":"https://doi.org/10.1051/ANALUSIS:2000280351","url":null,"abstract":"The analytical performance of the Fast - Protein Liquid Chromatography (FPLC) (on a Mono Q HR 5/5 column) and the conventional AnionExchange Chromatography (on a Protein Pak DEAE 5PW column) to realise rabbit liver metallothioneins separations and Cd speciation has been investigated and the results are compared. The Mono Q column was found to provide higher resolution and reproducibility in shorter separation times than Protein Pak DEAE 5PW, a conventional anion-exchange column. Thus, FPLC is proposed as a very useful tool for the study of the polymorphism of metallothioneins.","PeriodicalId":8221,"journal":{"name":"Analusis","volume":"24 1","pages":"351-357"},"PeriodicalIF":0.0,"publicationDate":"2000-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78229198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2000-06-01DOI: 10.1051/ANALUSIS:2000280391
M. Yang, K. Wan, Deng-yun Chen
Metallothioneins (MT) is a low molecular weight, cysteine-rich, metal-binding protein that can be induced when cells and tissues are exposed to metal such as Cd, Zn, Cu and Hg. MT could also be induced by non-metallic stimuli including physiological, chemical and pathological stresses. It has been suggested that signalling molecules such as cAMP or Ca ++ might also induce MT. In the present study, the mode of induction of MT in the RH-35 cells was investigated with reference to the integrity of the cells. The degree of MT induction was measured by the Cd-saturation assay in which total Cd bound to the heat-stable MT was determined by the graphite furnace atomic absorption spectrometry. It was demonstrated that Zn and Cd could markedly increase cellular MT content. Alpha-amanitin, which blocks DNA transcription, can significantly decrease the Cd induced MT induction. MT induction was also lowered by cycloheximide, which blocks protein translation. However, dibutyl-cAMP, Ca ++ and Ca ++ in the presence of ionomycin were unable to induce cellular MT. Using this model, the results supported that notion that MT induction might not be mediated by cAMP or Ca ++ in this cell line.
{"title":"Evaluating the role of dibutyl-cAMP and Ca++ as an MT inducer in a clonal rat hepatoma cell","authors":"M. Yang, K. Wan, Deng-yun Chen","doi":"10.1051/ANALUSIS:2000280391","DOIUrl":"https://doi.org/10.1051/ANALUSIS:2000280391","url":null,"abstract":"Metallothioneins (MT) is a low molecular weight, cysteine-rich, metal-binding protein that can be induced when cells and tissues are exposed to metal such as Cd, Zn, Cu and Hg. MT could also be induced by non-metallic stimuli including physiological, chemical and pathological stresses. It has been suggested that signalling molecules such as cAMP or Ca ++ might also induce MT. In the present study, the mode of induction of MT in the RH-35 cells was investigated with reference to the integrity of the cells. The degree of MT induction was measured by the Cd-saturation assay in which total Cd bound to the heat-stable MT was determined by the graphite furnace atomic absorption spectrometry. It was demonstrated that Zn and Cd could markedly increase cellular MT content. Alpha-amanitin, which blocks DNA transcription, can significantly decrease the Cd induced MT induction. MT induction was also lowered by cycloheximide, which blocks protein translation. However, dibutyl-cAMP, Ca ++ and Ca ++ in the presence of ionomycin were unable to induce cellular MT. Using this model, the results supported that notion that MT induction might not be mediated by cAMP or Ca ++ in this cell line.","PeriodicalId":8221,"journal":{"name":"Analusis","volume":"55 1","pages":"391-395"},"PeriodicalIF":0.0,"publicationDate":"2000-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88855602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2000-06-01DOI: 10.1051/ANALUSIS:2000280386
M. Bebianno, M. Serafim, D. Simes
coastal waters. Due to its economic importance it is extensively cultured in many countries, and particularly in Portugal where its production represents > 80 % of total shellfish production. The selection of the clam R. decussatus as a bioindicator was a consequence of its economic importance and the need to use a filter-feeding bivalve to evaluate environmental changes of metal concentrations [1]. Like many other bivalves, this species resides in sediments and accumulates metal concentrations reflecting gradients of metal contamination in the surrounding environment [2,3,4]. This suggests that its tissues have mechanisms, related to its filter-feeding habit, that inhibit the toxic effects of these contaminants. Like many other molluscs R. decussatus has evolved a number of subcellular systems for accumulation, regulation and immobilization of metals. These include, among others, the binding of essential and pollutant metals to soluble ligands such as MT. These two-domain molecules are low molecular, heat-stable proteins of non-enzymatic nature that occur mainly in the cytoplasm. They have strong affinity to class B metal cations which enable them to be differentiated from most of the other proteins [5]. The induction of these proteins has, therefore, been proposed as a specific indicator and possible “early warning marker” for the detection of detrimental effects caused by exposure to excess of essential and pollutant metals. MT induction has been detected in the whole soft tissues, gills, digestive gland and remaining tissues of the clam R. decussatus after exposure to essential and toxic metals [4,6] and this paper represents an overview of MT function in this bivalve species. MT induction in laboratory experiments
{"title":"Metallothioneins in the clam Ruditapes decussatus: an overview","authors":"M. Bebianno, M. Serafim, D. Simes","doi":"10.1051/ANALUSIS:2000280386","DOIUrl":"https://doi.org/10.1051/ANALUSIS:2000280386","url":null,"abstract":"coastal waters. Due to its economic importance it is extensively cultured in many countries, and particularly in Portugal where its production represents > 80 % of total shellfish production. The selection of the clam R. decussatus as a bioindicator was a consequence of its economic importance and the need to use a filter-feeding bivalve to evaluate environmental changes of metal concentrations [1]. Like many other bivalves, this species resides in sediments and accumulates metal concentrations reflecting gradients of metal contamination in the surrounding environment [2,3,4]. This suggests that its tissues have mechanisms, related to its filter-feeding habit, that inhibit the toxic effects of these contaminants. Like many other molluscs R. decussatus has evolved a number of subcellular systems for accumulation, regulation and immobilization of metals. These include, among others, the binding of essential and pollutant metals to soluble ligands such as MT. These two-domain molecules are low molecular, heat-stable proteins of non-enzymatic nature that occur mainly in the cytoplasm. They have strong affinity to class B metal cations which enable them to be differentiated from most of the other proteins [5]. The induction of these proteins has, therefore, been proposed as a specific indicator and possible “early warning marker” for the detection of detrimental effects caused by exposure to excess of essential and pollutant metals. MT induction has been detected in the whole soft tissues, gills, digestive gland and remaining tissues of the clam R. decussatus after exposure to essential and toxic metals [4,6] and this paper represents an overview of MT function in this bivalve species. MT induction in laboratory experiments","PeriodicalId":8221,"journal":{"name":"Analusis","volume":"32 1","pages":"386-390"},"PeriodicalIF":0.0,"publicationDate":"2000-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91208870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2000-06-01DOI: 10.1051/ANALUSIS:2000120
J. Kaloustian, A. Pauli, J. Pastor
Essential oils are used in perfumes, cooking and in pharmacy. A special study had been done on essential oils issued from two plants of Lavendin and Rosemary, during the annual biological cycle. The GC/MS technique was used and identifica- tion of separated components was achieved by retention times in comparison with respective standards and by mass spectra com- parison. Quantitative determinations of camphor, cineol, linalool, linalyl acetate in Lavendin, and camphor, α-pinene, cineol in Rosemary, were done by GC/FID. n-Dodecane was used as internal standard. The aim of this study was to choose the optimum period of plant gathering, with respect to the level of camphor and other major components. An increasing of the camphor is observed in the leaves. Camphor, present in essential oils of Lavendin and Rosemary, has new pharmaceutical applications, mainl y as stimulant, cardiac and respiratory analeptic.
{"title":"Evolution of camphor and others components in the essential oils of two labiate species during the biological cycle.","authors":"J. Kaloustian, A. Pauli, J. Pastor","doi":"10.1051/ANALUSIS:2000120","DOIUrl":"https://doi.org/10.1051/ANALUSIS:2000120","url":null,"abstract":"Essential oils are used in perfumes, cooking and in pharmacy. A special study had been done on essential oils issued from two plants of Lavendin and Rosemary, during the annual biological cycle. The GC/MS technique was used and identifica- tion of separated components was achieved by retention times in comparison with respective standards and by mass spectra com- parison. Quantitative determinations of camphor, cineol, linalool, linalyl acetate in Lavendin, and camphor, α-pinene, cineol in Rosemary, were done by GC/FID. n-Dodecane was used as internal standard. The aim of this study was to choose the optimum period of plant gathering, with respect to the level of camphor and other major components. An increasing of the camphor is observed in the leaves. Camphor, present in essential oils of Lavendin and Rosemary, has new pharmaceutical applications, mainl y as stimulant, cardiac and respiratory analeptic.","PeriodicalId":8221,"journal":{"name":"Analusis","volume":"30 1","pages":"308-315"},"PeriodicalIF":0.0,"publicationDate":"2000-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89781356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2000-06-01DOI: 10.1051/ANALUSIS:2000123
J. Pet'ka, P. Etiévant, G. Callement
Three types of plastic material (Saran, Tedlar and Teflon) were examined for their influence on the profile of a model headspace containing 13 volatile compounds of different volatility and polarity. As far as the emission of contaminants from the bags to the headspace is considered, the most appropriate material is obviously Teflon. However, the drawback of this material is a rapid sorption and then a slow adsorption of most volatile compounds tested, in particular polar ones. The authors suggest that acceptable time for headspace storage is between 4 and 12 hours, but with possible loss of the most polar compounds pre- sent in trace quantities.
{"title":"Suitability of different plastic materials for head or nose spaces short term storage","authors":"J. Pet'ka, P. Etiévant, G. Callement","doi":"10.1051/ANALUSIS:2000123","DOIUrl":"https://doi.org/10.1051/ANALUSIS:2000123","url":null,"abstract":"Three types of plastic material (Saran, Tedlar and Teflon) were examined for their influence on the profile of a model headspace containing 13 volatile compounds of different volatility and polarity. As far as the emission of contaminants from the bags to the headspace is considered, the most appropriate material is obviously Teflon. However, the drawback of this material is a rapid sorption and then a slow adsorption of most volatile compounds tested, in particular polar ones. The authors suggest that acceptable time for headspace storage is between 4 and 12 hours, but with possible loss of the most polar compounds pre- sent in trace quantities.","PeriodicalId":8221,"journal":{"name":"Analusis","volume":"24 1","pages":"330-335"},"PeriodicalIF":0.0,"publicationDate":"2000-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84594223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2000-06-01DOI: 10.1051/ANALUSIS:2000280370
M. Dabrio, A. Rodríguez
An electrochemical study of the complexing properties of the β metallothionein domain with cadmium and/or zinc was performed at pH 8, using differential pulse polarography. The applied technique allows one to obtain well-defined peaks corresponding to the free metal ions as well as providing evidence that different forms of complexation of Cd(II) and Zn(II) with the β domain exist. The influence of sequential additions of Cd and/or Zn to the β domain was investigated. The β domain is capable of complexing cadmium and/or zinc, separately or after simultaneous additions, resulting in complexes with a of M:β ratio of 3:1, but which possess different characteristics. In the case of binary mixtures, Cd + β or Zn + β, pure complexes with a probable stoichiometry of Cd3β and Zn3β are formed, whereas in the case of tertiary mixtures, β + [Cd + Zn] mixed cadmium and zinc complexes Cd2Znβ and CdZn2β prevail. When one of the two metal ions is added to a solution containing the β domain complex with the other cation, it is incorporated into the molecule resulting in a reorganisation, which is illustrated by changes in the polarographic responses. Polarographic results have been compared with those obtained by electrospray ionisation mass spectrometry, providing the molecular mass of compounds under similar experimental conditions. Different polarographic peaks are found for either pure or mixed complexes.
{"title":"■ Complexing properties of the β metallothionein domain with cadmium and/or zinc, studied by differential pulse polarography","authors":"M. Dabrio, A. Rodríguez","doi":"10.1051/ANALUSIS:2000280370","DOIUrl":"https://doi.org/10.1051/ANALUSIS:2000280370","url":null,"abstract":"An electrochemical study of the complexing properties of the β metallothionein domain with cadmium and/or zinc was performed at pH 8, using differential pulse polarography. The applied technique allows one to obtain well-defined peaks corresponding to the free metal ions as well as providing evidence that different forms of complexation of Cd(II) and Zn(II) with the β domain exist. The influence of sequential additions of Cd and/or Zn to the β domain was investigated. The β domain is capable of complexing cadmium and/or zinc, separately or after simultaneous additions, resulting in complexes with a of M:β ratio of 3:1, but which possess different characteristics. In the case of binary mixtures, Cd + β or Zn + β, pure complexes with a probable stoichiometry of Cd3β and Zn3β are formed, whereas in the case of tertiary mixtures, β + [Cd + Zn] mixed cadmium and zinc complexes Cd2Znβ and CdZn2β prevail. When one of the two metal ions is added to a solution containing the β domain complex with the other cation, it is incorporated into the molecule resulting in a reorganisation, which is illustrated by changes in the polarographic responses. Polarographic results have been compared with those obtained by electrospray ionisation mass spectrometry, providing the molecular mass of compounds under similar experimental conditions. Different polarographic peaks are found for either pure or mixed complexes.","PeriodicalId":8221,"journal":{"name":"Analusis","volume":"84 1","pages":"370-381"},"PeriodicalIF":0.0,"publicationDate":"2000-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90363843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2000-06-01DOI: 10.1051/ANALUSIS:2000280366
K. Kubo, Y. Sakita, T. Minami
The effect of heat treatment on metallothionein (MT) isoforms was observed by using capillary zone electrophoresis (CZE) at a neutral pH. Commercially available standard MT-1 preparation isolated from rabbit liver present as Cd and Zn mixed form was used as MT standard for the experiments in CZE. The MT-1 preparation was detected as two major peaks MT-1 and MT-2 identified from the migration times corresponded with those of purified isoforms and several minor peaks by CZE using a polyacrylamide-coated capillary at pH 7.4. The peak height of MT-1 of the standard MT-1 specimen decreased gradually from those of the non-heated specimen by heat treatment at 100°C until 4 min, but the peak of MT-2 did not decrease. In the cytosol fraction obtained from Zn-injected mouse liver, many peaks including MT-1 and MT-2 were detected in the specimen without heat treatment. The specimen heated at 100°C for 15 s had a stable baseline and the peaks of both MT-1 and MT-2 in cytosol fraction decreased gradually by heat treatment on time dependent manner. However, the ratio of peak heights of MT-1 and MT-2 did not change. From these results, the ratio of MT-1 and MT-2 in cytosol fraction is able to be observed well on CZE analysis after heat treatment.
{"title":"Effect of heat treatment on metallothionein isoforms using capillary zone electrophoresis","authors":"K. Kubo, Y. Sakita, T. Minami","doi":"10.1051/ANALUSIS:2000280366","DOIUrl":"https://doi.org/10.1051/ANALUSIS:2000280366","url":null,"abstract":"The effect of heat treatment on metallothionein (MT) isoforms was observed by using capillary zone electrophoresis (CZE) at a neutral pH. Commercially available standard MT-1 preparation isolated from rabbit liver present as Cd and Zn mixed form was used as MT standard for the experiments in CZE. The MT-1 preparation was detected as two major peaks MT-1 and MT-2 identified from the migration times corresponded with those of purified isoforms and several minor peaks by CZE using a polyacrylamide-coated capillary at pH 7.4. The peak height of MT-1 of the standard MT-1 specimen decreased gradually from those of the non-heated specimen by heat treatment at 100°C until 4 min, but the peak of MT-2 did not decrease. In the cytosol fraction obtained from Zn-injected mouse liver, many peaks including MT-1 and MT-2 were detected in the specimen without heat treatment. The specimen heated at 100°C for 15 s had a stable baseline and the peaks of both MT-1 and MT-2 in cytosol fraction decreased gradually by heat treatment on time dependent manner. However, the ratio of peak heights of MT-1 and MT-2 did not change. From these results, the ratio of MT-1 and MT-2 in cytosol fraction is able to be observed well on CZE analysis after heat treatment.","PeriodicalId":8221,"journal":{"name":"Analusis","volume":"21 1","pages":"366-369"},"PeriodicalIF":0.0,"publicationDate":"2000-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86241528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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