Abstract In this study, we investigated the dietary effects of dandelion root extract (DRE) on growth performance, body composition, serum biochemical parameters, intestinal and liver histology, and fish resistance against Lactococcus garvieae infection in the rainbow trout. In total, 540 rainbow trout fry (22.05±1.740 g and 13.03±0.470 cm) were used. Six experimental groups (Control, 2.5 mL/kg (DRE-1), 5 mL/kg (DRE-2), 10 mL/kg (DRE-3), 20 mL/kg (DRE-4), and 40 mL/kg (DRE-5) dandelion root extract) with three replicates were formed. In each tank, 30 fish were placed and fed for 90 days, and the volume of water was adjusted to 80 L with a flow-through system. The results showed that the final fish weight, weight gain, specific growth rate, protein efficiency ratio, and protein deposition rate were significantly higher in the DRE-4 group, while the feed conversion ratio and fat deposition ratio were significantly lower compared to that in the control group (P<0.05). The viscerosomatic index and the hepatosomatic index decreased in the experimental groups depending on the concentration of the extract used, while the condition factor was higher than that in the control (P<0.05). The DRE significantly decreased glucose, cholesterol, triglyceride, low-density lipoprotein, alanine aminotransferase, and aspartate aminotransferase levels in blood serum in the fish from the experimental groups, but it significantly increased high-density lipoprotein, alkaline phosphatase, and total protein levels (P<0.05). Similarly, the DRE positively affected intestinal histology by increasing the stratum compactum, submucosa, lamina propria, villi length, villi width, absorption area, tunica muscularis thickness, mucosal folds, and goblet cell count. In the liver, depending on the high-fat diet (crude lipid = 20%), vacuolization areas, hepatocellular degeneration areas, apoptotic hepatocyte nucleus, and necrotic areas recorded in the control group decreased or even disappeared completely in the extract groups. The results of the challenge test showed that adding 2.5 mL/kg of the DRE to the feed positively affected the disease resistance against Lactococcus garvieae infection. The results of the quadratic polynomial regression showed that an average level of 23.91±1.048 mL/kg of DRE would be suitable for optimum fish growth.
{"title":"Dandelion root extract in trout feed and its effects on the physiological performance of Oncorhynchus mykiss and resistance to Lactococcus garvieae infection","authors":"Ö. Köse, Huriye Ariman Karabulut, A. Er","doi":"10.2478/aoas-2023-0072","DOIUrl":"https://doi.org/10.2478/aoas-2023-0072","url":null,"abstract":"Abstract In this study, we investigated the dietary effects of dandelion root extract (DRE) on growth performance, body composition, serum biochemical parameters, intestinal and liver histology, and fish resistance against Lactococcus garvieae infection in the rainbow trout. In total, 540 rainbow trout fry (22.05±1.740 g and 13.03±0.470 cm) were used. Six experimental groups (Control, 2.5 mL/kg (DRE-1), 5 mL/kg (DRE-2), 10 mL/kg (DRE-3), 20 mL/kg (DRE-4), and 40 mL/kg (DRE-5) dandelion root extract) with three replicates were formed. In each tank, 30 fish were placed and fed for 90 days, and the volume of water was adjusted to 80 L with a flow-through system. The results showed that the final fish weight, weight gain, specific growth rate, protein efficiency ratio, and protein deposition rate were significantly higher in the DRE-4 group, while the feed conversion ratio and fat deposition ratio were significantly lower compared to that in the control group (P<0.05). The viscerosomatic index and the hepatosomatic index decreased in the experimental groups depending on the concentration of the extract used, while the condition factor was higher than that in the control (P<0.05). The DRE significantly decreased glucose, cholesterol, triglyceride, low-density lipoprotein, alanine aminotransferase, and aspartate aminotransferase levels in blood serum in the fish from the experimental groups, but it significantly increased high-density lipoprotein, alkaline phosphatase, and total protein levels (P<0.05). Similarly, the DRE positively affected intestinal histology by increasing the stratum compactum, submucosa, lamina propria, villi length, villi width, absorption area, tunica muscularis thickness, mucosal folds, and goblet cell count. In the liver, depending on the high-fat diet (crude lipid = 20%), vacuolization areas, hepatocellular degeneration areas, apoptotic hepatocyte nucleus, and necrotic areas recorded in the control group decreased or even disappeared completely in the extract groups. The results of the challenge test showed that adding 2.5 mL/kg of the DRE to the feed positively affected the disease resistance against Lactococcus garvieae infection. The results of the quadratic polynomial regression showed that an average level of 23.91±1.048 mL/kg of DRE would be suitable for optimum fish growth.","PeriodicalId":8235,"journal":{"name":"Annals of Animal Science","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2023-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43048333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Starvation and malnutrition are one of the most destructive problems faced by the poor and needy in the world. With the world population expected to increase by 9.7 billion by 2050, there are growing concerns about the long-term sustainability of many existing food production systems to meet future needs for food. Aquaculture is one of the important sources of food supply, the development of which is of great importance as an important weapon in the global fight against malnutrition and poverty, especially in developing countries and arid and semi-arid regions of the world. In recent decades, aquaculture has dominated all sectors of livestock production in terms of growth and increasingly contributes to food security, poverty reduction, job creation and sources of income. It is important to follow sustainable development goals to increase aquaculture production. Climate change is currently affecting food security through increasing temperature and decreasing rainfall, especially in arid and semi-arid regions. Food security is a global concern and access to affordable, nutritious, safe and properly cultivated food is one of the most important issues globally. The main emphasis in the development of aquaculture in arid and semi-arid areas is the use of new technologies based on water saving, such as biofloc technology (BFT), integrated multi-trophic aquaculture system (IMTA) and recirculating aquaculture systems (RAS). In this study, the importance of aquaculture as a sustainable source of food production in arid and semi-arid regions is discussed.
{"title":"Strategies for promoting sustainable aquaculture in arid and semi-arid areas","authors":"M. Khanjani, M. Sharifinia, S. Hajirezaee","doi":"10.2478/aoas-2023-0073","DOIUrl":"https://doi.org/10.2478/aoas-2023-0073","url":null,"abstract":"Abstract Starvation and malnutrition are one of the most destructive problems faced by the poor and needy in the world. With the world population expected to increase by 9.7 billion by 2050, there are growing concerns about the long-term sustainability of many existing food production systems to meet future needs for food. Aquaculture is one of the important sources of food supply, the development of which is of great importance as an important weapon in the global fight against malnutrition and poverty, especially in developing countries and arid and semi-arid regions of the world. In recent decades, aquaculture has dominated all sectors of livestock production in terms of growth and increasingly contributes to food security, poverty reduction, job creation and sources of income. It is important to follow sustainable development goals to increase aquaculture production. Climate change is currently affecting food security through increasing temperature and decreasing rainfall, especially in arid and semi-arid regions. Food security is a global concern and access to affordable, nutritious, safe and properly cultivated food is one of the most important issues globally. The main emphasis in the development of aquaculture in arid and semi-arid areas is the use of new technologies based on water saving, such as biofloc technology (BFT), integrated multi-trophic aquaculture system (IMTA) and recirculating aquaculture systems (RAS). In this study, the importance of aquaculture as a sustainable source of food production in arid and semi-arid regions is discussed.","PeriodicalId":8235,"journal":{"name":"Annals of Animal Science","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2023-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43465965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. Gufe, D. Merrifield, S. Hoseinifar, T. Rattanarojpong, P. Khunrae, M. Abdel‐Tawwab
Abstract Xylooligosaccharides (XOSs) are increasingly being explored as prebiotics in fish diets; however, their effects and modes of action have not been well evaluated. Reports have shown that dietary XOS has the potential to improve the proliferation of beneficial gut microbes, and their metabolites, and enhance disease resistance in several fish species. In contrast, other studies report no substantial changes in immune and growth parameters compared to control groups. Like all prebiotics, the mode of action of XOS is based on their selective stimulation of beneficial gut microbiota, which will outcompete and prevent pathogen proliferation in the gut, and produce metabolites that modulate host immune responses. The reports of improved growth performance of XOS fed fish may be due improved intestinal microbiome, enhanced glycolysis activity and elevated gastrointestinal enzymatic activities. Dietary XOSs have different effects on fish performance depending on the fish species and the structure of XOSs (degree of XOS polymerization and substitution). Nevertheless, further research is essential to determine the optimal dosage, degree of polymerization, and substitution levels required to improve each fish species' gut health and growth performance. This review highlights the prebiotic effects of XOSs, their mechanism of action, and knowledge gaps.
{"title":"Prebiotic effects of dietary xylooligosaccharides on fish gut microbiota, growth, and immunological parameters – a review","authors":"C. Gufe, D. Merrifield, S. Hoseinifar, T. Rattanarojpong, P. Khunrae, M. Abdel‐Tawwab","doi":"10.2478/aoas-2023-0069","DOIUrl":"https://doi.org/10.2478/aoas-2023-0069","url":null,"abstract":"Abstract Xylooligosaccharides (XOSs) are increasingly being explored as prebiotics in fish diets; however, their effects and modes of action have not been well evaluated. Reports have shown that dietary XOS has the potential to improve the proliferation of beneficial gut microbes, and their metabolites, and enhance disease resistance in several fish species. In contrast, other studies report no substantial changes in immune and growth parameters compared to control groups. Like all prebiotics, the mode of action of XOS is based on their selective stimulation of beneficial gut microbiota, which will outcompete and prevent pathogen proliferation in the gut, and produce metabolites that modulate host immune responses. The reports of improved growth performance of XOS fed fish may be due improved intestinal microbiome, enhanced glycolysis activity and elevated gastrointestinal enzymatic activities. Dietary XOSs have different effects on fish performance depending on the fish species and the structure of XOSs (degree of XOS polymerization and substitution). Nevertheless, further research is essential to determine the optimal dosage, degree of polymerization, and substitution levels required to improve each fish species' gut health and growth performance. This review highlights the prebiotic effects of XOSs, their mechanism of action, and knowledge gaps.","PeriodicalId":8235,"journal":{"name":"Annals of Animal Science","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2023-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47929085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Livestock farming is considered one relevant cause of global warming. The aim of this article is to show that the environmental footprint of broilers and laying hens is small among farmed animal species. Author examines the increase in the production of broilers and laying hens over the past decades as a result of genetic selection, and as a consequence their greenhouse gas emissions, land, water and energy use have decreased. It was evidenced that with the increase of production the environmental footprint per unit of product decreases. On the other hand, in alternative housing systems, especially in free-range and organic production, all indicators deteriorate. Supplying the Earth's ever-growing population with a sufficient amount of high-quality food of animal origin, in such a way that the environmental footprint of poultry farming is reduced, can be achieved with intensive production taking into account animal welfare. New tools and methods such as genomic selection, gene editing or precision poultry breeding will help in this.
{"title":"Poultry farming does not play a significant role in global warming","authors":"Z. Szendrő","doi":"10.2478/aoas-2023-0074","DOIUrl":"https://doi.org/10.2478/aoas-2023-0074","url":null,"abstract":"Abstract Livestock farming is considered one relevant cause of global warming. The aim of this article is to show that the environmental footprint of broilers and laying hens is small among farmed animal species. Author examines the increase in the production of broilers and laying hens over the past decades as a result of genetic selection, and as a consequence their greenhouse gas emissions, land, water and energy use have decreased. It was evidenced that with the increase of production the environmental footprint per unit of product decreases. On the other hand, in alternative housing systems, especially in free-range and organic production, all indicators deteriorate. Supplying the Earth's ever-growing population with a sufficient amount of high-quality food of animal origin, in such a way that the environmental footprint of poultry farming is reduced, can be achieved with intensive production taking into account animal welfare. New tools and methods such as genomic selection, gene editing or precision poultry breeding will help in this.","PeriodicalId":8235,"journal":{"name":"Annals of Animal Science","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2023-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49652425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Antimicrobial peptides (AMPs) are the focus of this manuscript, as bioconservative molecules that constitute a major defense mechanism in many organisms. Various antimicrobial peptides have been isolated and identified, but AMPs derived from Hermetia illucens (HI) will be the focus of this review. The review focuses mainly on cecropins, defensins, and attacins. Hermetia illucens is a remarkable organism adapted to life in a diverse, often highly polluted environment, and its resilience is largely attributed to AMPs. AMPs are active against many bacterial and fungal species and also act to induce the osmotic lysis of protozoa. They attack pathogenic microorganisms without damaging host cells in the process. Given the increasing antibiotic resistance of many bacterial strains in animal production, AMPs appear to be a tempting alternative as substances that limit and inhibit the growth and development of pathogens, as well as an option in veterinary medicine as potential substitutes for antibiotics, the administration of which must be limited in the European Union. The AMP content of HI larvae extracts, which determines their activity, depends on the larval diet and the solvent used. This review presents in vitro studies on the effects of AMPs from Hermetia illucens on microorganisms and in vivo studies on the potential of HI larvae meal as a feed supplement.
{"title":"Hermetia illucens as a source of antimicrobial peptides – a review of in vitro and in vivo studies","authors":"Kinga Szczepanik, M. Świątkiewicz","doi":"10.2478/aoas-2023-0071","DOIUrl":"https://doi.org/10.2478/aoas-2023-0071","url":null,"abstract":"Abstract Antimicrobial peptides (AMPs) are the focus of this manuscript, as bioconservative molecules that constitute a major defense mechanism in many organisms. Various antimicrobial peptides have been isolated and identified, but AMPs derived from Hermetia illucens (HI) will be the focus of this review. The review focuses mainly on cecropins, defensins, and attacins. Hermetia illucens is a remarkable organism adapted to life in a diverse, often highly polluted environment, and its resilience is largely attributed to AMPs. AMPs are active against many bacterial and fungal species and also act to induce the osmotic lysis of protozoa. They attack pathogenic microorganisms without damaging host cells in the process. Given the increasing antibiotic resistance of many bacterial strains in animal production, AMPs appear to be a tempting alternative as substances that limit and inhibit the growth and development of pathogens, as well as an option in veterinary medicine as potential substitutes for antibiotics, the administration of which must be limited in the European Union. The AMP content of HI larvae extracts, which determines their activity, depends on the larval diet and the solvent used. This review presents in vitro studies on the effects of AMPs from Hermetia illucens on microorganisms and in vivo studies on the potential of HI larvae meal as a feed supplement.","PeriodicalId":8235,"journal":{"name":"Annals of Animal Science","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2023-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49539763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. F. Fath El-Bab, A. Amer, Mohamed M. El-Nawsany, Ibrahim H. Ibrahim, Ali H. Gouda, A. A. El-Bahlol, M. Naiel
Abstract The main objective of the current trial was to evaluate the beneficial influences of Origanum vulgare leaf extract (OVLE) on growth, some blood indices, redox status, and the expression of some growth and immune-related genes. Three thousand seven hundred and eighty Nile tilapia fingerlings were allocated six equal groups. The first group served as a control and received a basal diet, while the other five groups were fed diets containing graded amounts of OVLE (0.1, 0.2, 0.3, 0.4, and 0.5 %, respectively) and defined as OVLE0.1, OVLE0.2, OVLE0.3, OVLE0.4, and OVLE0.5 for 98 days. Growth performance and feed efficiency parameters were significantly improved in OVLE0.4 and OVLE0.5 groups compared with the control group. The hematological indices, specifically hemoglobin, red blood cell, and white blood cell count were significantly increased in the fish group fed a 4% OVLE-supplemented diet compared to other groups. Fish fed higher dietary inclusion levels of OVLE significantly increased total protein and albumin concentrations compared to other experimental groups. The OVLE0.4 and OVLE0.5 supplemented groups promoted innate immune response and phagocytic activity and stimulated the lipase enzyme activity of Nile tilapia. All estimated redox markers were significantly improved in the fish groups that received 4 or 5 % OVLE compared with the control and other OVLE groups. The fish groups fed higher levels of OVLE significantly up-regulated the expression of GH, IL-8, and IL-1β genes and downregulated the expression of the Hsp70 gene. Furthermore, the intestinal histological features demonstrated normal structure in all OVLE-administered fish groups, with improved intestinal villus length compared to the untreated group. Finally, it could be concluded that including OVLE in the Nile tilapia diet at higher levels (0.4 up to 0.5%) could improve performance and general health status.
{"title":"Oregano leaf extract dietary administration modulates performance, redox status, intestinal health, and expression of some related genes of Nile tilapia (Oreochromis niloticus L.)","authors":"A. F. Fath El-Bab, A. Amer, Mohamed M. El-Nawsany, Ibrahim H. Ibrahim, Ali H. Gouda, A. A. El-Bahlol, M. Naiel","doi":"10.2478/aoas-2023-0068","DOIUrl":"https://doi.org/10.2478/aoas-2023-0068","url":null,"abstract":"Abstract The main objective of the current trial was to evaluate the beneficial influences of Origanum vulgare leaf extract (OVLE) on growth, some blood indices, redox status, and the expression of some growth and immune-related genes. Three thousand seven hundred and eighty Nile tilapia fingerlings were allocated six equal groups. The first group served as a control and received a basal diet, while the other five groups were fed diets containing graded amounts of OVLE (0.1, 0.2, 0.3, 0.4, and 0.5 %, respectively) and defined as OVLE0.1, OVLE0.2, OVLE0.3, OVLE0.4, and OVLE0.5 for 98 days. Growth performance and feed efficiency parameters were significantly improved in OVLE0.4 and OVLE0.5 groups compared with the control group. The hematological indices, specifically hemoglobin, red blood cell, and white blood cell count were significantly increased in the fish group fed a 4% OVLE-supplemented diet compared to other groups. Fish fed higher dietary inclusion levels of OVLE significantly increased total protein and albumin concentrations compared to other experimental groups. The OVLE0.4 and OVLE0.5 supplemented groups promoted innate immune response and phagocytic activity and stimulated the lipase enzyme activity of Nile tilapia. All estimated redox markers were significantly improved in the fish groups that received 4 or 5 % OVLE compared with the control and other OVLE groups. The fish groups fed higher levels of OVLE significantly up-regulated the expression of GH, IL-8, and IL-1β genes and downregulated the expression of the Hsp70 gene. Furthermore, the intestinal histological features demonstrated normal structure in all OVLE-administered fish groups, with improved intestinal villus length compared to the untreated group. Finally, it could be concluded that including OVLE in the Nile tilapia diet at higher levels (0.4 up to 0.5%) could improve performance and general health status.","PeriodicalId":8235,"journal":{"name":"Annals of Animal Science","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2023-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44594018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Szulc, Bogumiła Nowak, M. Hassan, D. Lechniak, S. Ślusarczyk, J. Bocianowski, M. Szumacher-Strabel, A. Patra, A. Cieślak
Abstract Environmental impact, quality, and quantity of food products of ruminant origin (especially beef and mutton) are considered major challenges in meeting the nutritional requirements of the growing human population worldwide. Therefore, we conducted this in vitro study to explore the potential of Paulownia leaves silage (PLS) to reduce the environmental impact of feedlot lamb production and improve ruminal fatty acids (FAs) profile by influencing ruminal biohydrogenation. In the present study, Paulownia leaves silage (PLS) and alfalfa silage (AAS) were mixed in a proportion of 1:0 (Control, PLS 0%), 0.75:0.25 (PLS 25%), 0.5:0.5 (PLS 50%), 0.25:0.75 (PLS 75%) and 0:1 (PLS 100%) on dry matter (DM) basis in the lamb diet. The experimental findings demonstrated that 100% replacement of AAS with PLS in the lamb diet significantly improved the ruminal fermentation by increasing the in vitro DM degradability (P<0.01), total volatile fatty acid (P<0.01), and propionate production (P<0.01) while reducing the acetate: propionate (A/P) ratio (P<0.01) and CH4 concentration (mM; L and Q P<0.05) and CH4, mM/g DM (L and Q P<0.05). Meanwhile, 100% PLS inclusion in the diet increased the total monounsaturated fatty acids (P<0.05), total unsaturated fatty acids (P<0.01) and total n-3 fatty acids (P<0.05) particularly alpha-linolenic acid (ALA; C18:3 n-3; P<0.05) in the rumen fluid after incubation. Moreover, the total saturated fatty acids concentration was reduced (P<0.01). These findings suggested that PLS could be a climate-friendly and sustainable alternative to AAS in the lamb feedlot diet for quality meat production.
{"title":"Potential of Paulownia leaves silage in lamb diet to improve ruminal fermentation and fatty acid profile – an in vitro study","authors":"P. Szulc, Bogumiła Nowak, M. Hassan, D. Lechniak, S. Ślusarczyk, J. Bocianowski, M. Szumacher-Strabel, A. Patra, A. Cieślak","doi":"10.2478/aoas-2023-0065","DOIUrl":"https://doi.org/10.2478/aoas-2023-0065","url":null,"abstract":"Abstract Environmental impact, quality, and quantity of food products of ruminant origin (especially beef and mutton) are considered major challenges in meeting the nutritional requirements of the growing human population worldwide. Therefore, we conducted this in vitro study to explore the potential of Paulownia leaves silage (PLS) to reduce the environmental impact of feedlot lamb production and improve ruminal fatty acids (FAs) profile by influencing ruminal biohydrogenation. In the present study, Paulownia leaves silage (PLS) and alfalfa silage (AAS) were mixed in a proportion of 1:0 (Control, PLS 0%), 0.75:0.25 (PLS 25%), 0.5:0.5 (PLS 50%), 0.25:0.75 (PLS 75%) and 0:1 (PLS 100%) on dry matter (DM) basis in the lamb diet. The experimental findings demonstrated that 100% replacement of AAS with PLS in the lamb diet significantly improved the ruminal fermentation by increasing the in vitro DM degradability (P<0.01), total volatile fatty acid (P<0.01), and propionate production (P<0.01) while reducing the acetate: propionate (A/P) ratio (P<0.01) and CH4 concentration (mM; L and Q P<0.05) and CH4, mM/g DM (L and Q P<0.05). Meanwhile, 100% PLS inclusion in the diet increased the total monounsaturated fatty acids (P<0.05), total unsaturated fatty acids (P<0.01) and total n-3 fatty acids (P<0.05) particularly alpha-linolenic acid (ALA; C18:3 n-3; P<0.05) in the rumen fluid after incubation. Moreover, the total saturated fatty acids concentration was reduced (P<0.01). These findings suggested that PLS could be a climate-friendly and sustainable alternative to AAS in the lamb feedlot diet for quality meat production.","PeriodicalId":8235,"journal":{"name":"Annals of Animal Science","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2023-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45637077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Kropiwiec-Domańska, M. Babicz, M. Szyndler-Nędza, M. Tyra, E. Skrzypczak
Abstract The objective of this study was to comparatively analyse the physical parameters, basic chemical composition and the macro- and microelement content of offal obtained from fattening pigs raised in deep litter and slatted floor housing systems. The experiment involved a group of 80 Puławska fatteners, including 40 pigs kept in deep-litter pens (group I) and 40 pigs in slatted pens without bedding (group II). Offal (tongues, kidneys, hearts, lungs and livers) were evaluated for weight, physical characteristics (pH45, pH24, WHC), chemical composition (water, protein, fat) and the content of minerals: macro- (K, Na, Mg, Ca) and microelements (Zn, Fe, Mn, Cu). Based on the results obtained, associations were found between housing system and basic chemical composition of some offal, i.e. percentage of water (slatted floordeep litter) in lungs, content of protein (slatted floor>deep litter) and fat (slatted floordeep litter); lungs: sodium (slatted floor>deep litter), potassium (slatted floordeep litter) and copper (slatted floor
{"title":"Analysis of physical parameters and chemical composition of offal from Puławska fattening pigs raised in deep litter and slatted floor housing systems","authors":"K. Kropiwiec-Domańska, M. Babicz, M. Szyndler-Nędza, M. Tyra, E. Skrzypczak","doi":"10.2478/aoas-2023-0070","DOIUrl":"https://doi.org/10.2478/aoas-2023-0070","url":null,"abstract":"Abstract The objective of this study was to comparatively analyse the physical parameters, basic chemical composition and the macro- and microelement content of offal obtained from fattening pigs raised in deep litter and slatted floor housing systems. The experiment involved a group of 80 Puławska fatteners, including 40 pigs kept in deep-litter pens (group I) and 40 pigs in slatted pens without bedding (group II). Offal (tongues, kidneys, hearts, lungs and livers) were evaluated for weight, physical characteristics (pH45, pH24, WHC), chemical composition (water, protein, fat) and the content of minerals: macro- (K, Na, Mg, Ca) and microelements (Zn, Fe, Mn, Cu). Based on the results obtained, associations were found between housing system and basic chemical composition of some offal, i.e. percentage of water (slatted floor<deep litter) and protein (slatted floor>deep litter) in lungs, content of protein (slatted floor>deep litter) and fat (slatted floor<deep litter) in liver, and content of fat in heart (slatted floor<deep litter). The amount of macro- and microelements was significantly differentiated by the housing system with regard to the kidneys: potassium, magnesium, calcium and manganese (slatted floor<deep litter), sodium (slatted floor>deep litter); lungs: sodium (slatted floor>deep litter), potassium (slatted floor<deep litter); and liver: iron (slatted floor>deep litter) and copper (slatted floor<deep litter). In general, the housing system of fattening pigs modified the physical parameters and chemical composition of the analysed offal.","PeriodicalId":8235,"journal":{"name":"Annals of Animal Science","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2023-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46763056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Krzysztof Kowal, Angelika Tkaczyk-Wlizło, J. Gawor, B. Ślaska
Abstract The aim of this study was to identify molecular defects caused by mutations in mitochondrial DNA in cases of recurrent and multiple canine tumours. We presented molecular differences in the mtDNA genome for two tumours observed in different body parts of 5 dogs and throughout time in the case of the recurrence. Mitochondrial DNA was sequenced on an Illumina MiSeq sequencer using a 600-cycle kit in a paired-end mode targeting at least 100x coverage. The sequences obtained were subjected to bioinformatic analyses in order to determine mutation and polymorphic sites within the analysed mtDNA genome in the tumour tissue. The total amount of changes: single nucleotide polymorphisms (SNPs), indels, mutations, and heteroplasmy detected in this study was 329. Ten polymorphisms were found in all analysed samples: ins.2679_2680G (tRNA-Leu (UUR)), m.5367C>T (COX1), m.5444T>C (COX1), m.6065A>G (COX1), m.8368C>T (ATP6), m.8807G>A (COX3), ins.9913_9914TG (ND4L), m.13299T>A (ND5), m.15814C>T, and m.16418A>G (control region). Interestingly, the highest number of differences in the mtDNA genome was observed between non-cancerous pyogranuloma tissue and epithelioma glandulae sebacei. The mutations in the non-cancerous tissue were mainly found in positions where polymorphisms were observed in blood and tumour tissue. The lowest number of changes was observed for the youngest analysed dog, which may indicate that some changes appeared in the mitogenomes with age. There were fewer heteroplasmic alterations in the larger than smaller tumour, which may suggest that the tumour growth is enhanced by genomic stability. The changes in the protein-coding genes were mostly synonymous, and nonsynonymous changes did not lead to alterations in protein properties. New mutations were observed in the post-recurrence tumours in comparison with the pre-recurrent tissue and blood.
{"title":"Molecular differences in mitochondrial genomes (mitogenomes) of dogs with recurrent and multiple tumours and their reference to the human mitochondrial genome","authors":"Krzysztof Kowal, Angelika Tkaczyk-Wlizło, J. Gawor, B. Ślaska","doi":"10.2478/aoas-2023-0067","DOIUrl":"https://doi.org/10.2478/aoas-2023-0067","url":null,"abstract":"Abstract The aim of this study was to identify molecular defects caused by mutations in mitochondrial DNA in cases of recurrent and multiple canine tumours. We presented molecular differences in the mtDNA genome for two tumours observed in different body parts of 5 dogs and throughout time in the case of the recurrence. Mitochondrial DNA was sequenced on an Illumina MiSeq sequencer using a 600-cycle kit in a paired-end mode targeting at least 100x coverage. The sequences obtained were subjected to bioinformatic analyses in order to determine mutation and polymorphic sites within the analysed mtDNA genome in the tumour tissue. The total amount of changes: single nucleotide polymorphisms (SNPs), indels, mutations, and heteroplasmy detected in this study was 329. Ten polymorphisms were found in all analysed samples: ins.2679_2680G (tRNA-Leu (UUR)), m.5367C>T (COX1), m.5444T>C (COX1), m.6065A>G (COX1), m.8368C>T (ATP6), m.8807G>A (COX3), ins.9913_9914TG (ND4L), m.13299T>A (ND5), m.15814C>T, and m.16418A>G (control region). Interestingly, the highest number of differences in the mtDNA genome was observed between non-cancerous pyogranuloma tissue and epithelioma glandulae sebacei. The mutations in the non-cancerous tissue were mainly found in positions where polymorphisms were observed in blood and tumour tissue. The lowest number of changes was observed for the youngest analysed dog, which may indicate that some changes appeared in the mitogenomes with age. There were fewer heteroplasmic alterations in the larger than smaller tumour, which may suggest that the tumour growth is enhanced by genomic stability. The changes in the protein-coding genes were mostly synonymous, and nonsynonymous changes did not lead to alterations in protein properties. New mutations were observed in the post-recurrence tumours in comparison with the pre-recurrent tissue and blood.","PeriodicalId":8235,"journal":{"name":"Annals of Animal Science","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2023-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44764732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. K. Aldal'in, Khadija S. Radhi, Reem S. Alazragi, S. Abdelnour, M. Abukhalil, Ahmed Askar, N. E. Khalifa, A. Noreldin, O. Y. Althunibat, M. Arif, M. A. Abd El-Hack
Abstract In the nanotechnology era, nanotechnology applications have been intensifying their prospects to embrace all the vigorous sectors persuading human health and animal. The safety and concerns regarding the widespread use of engineered nanomaterials (NMs) and their potential effect on human health still require further clarification. Literature elucidated that NMs exhibited significant adverse effects on various molecular and cellular alterations. Epigenetics is a complex process resulting in the interactions between an organism’s environment and genome. The epigenetic modifications, including histone modification and DNA methylation, chromatin structure and DNA accessibility alteration, regulate gene expression patterns. Disturbances of epigenetic markers induced by NMs might promote the sensitivity of humans and animals to several diseases. Also, this paper focuses on the epigenetic regulators of some dietary nutrients that have been confirmed to stimulate the epigenome and, more exactly, DNA histone modifications and non-histone proteins modulation by acetylation, and phosphorylation inhibition, which counteracts oxidative stress generations. The present review epitomizes the recent evidence of the potential effects of NMs on histone modifications, in addition to in vivo and in vitro cytosine DNA methylation and its toxicity. Furthermore, the part of epigenetic fluctuations as possible translational biomarkers for uncovering untoward properties of NMs is deliberated.
{"title":"A review on the epigenetics modifications to nanomaterials in humans and animals: Novel epigenetic regulator","authors":"H. K. Aldal'in, Khadija S. Radhi, Reem S. Alazragi, S. Abdelnour, M. Abukhalil, Ahmed Askar, N. E. Khalifa, A. Noreldin, O. Y. Althunibat, M. Arif, M. A. Abd El-Hack","doi":"10.2478/aoas-2023-0089","DOIUrl":"https://doi.org/10.2478/aoas-2023-0089","url":null,"abstract":"Abstract In the nanotechnology era, nanotechnology applications have been intensifying their prospects to embrace all the vigorous sectors persuading human health and animal. The safety and concerns regarding the widespread use of engineered nanomaterials (NMs) and their potential effect on human health still require further clarification. Literature elucidated that NMs exhibited significant adverse effects on various molecular and cellular alterations. Epigenetics is a complex process resulting in the interactions between an organism’s environment and genome. The epigenetic modifications, including histone modification and DNA methylation, chromatin structure and DNA accessibility alteration, regulate gene expression patterns. Disturbances of epigenetic markers induced by NMs might promote the sensitivity of humans and animals to several diseases. Also, this paper focuses on the epigenetic regulators of some dietary nutrients that have been confirmed to stimulate the epigenome and, more exactly, DNA histone modifications and non-histone proteins modulation by acetylation, and phosphorylation inhibition, which counteracts oxidative stress generations. The present review epitomizes the recent evidence of the potential effects of NMs on histone modifications, in addition to in vivo and in vitro cytosine DNA methylation and its toxicity. Furthermore, the part of epigenetic fluctuations as possible translational biomarkers for uncovering untoward properties of NMs is deliberated.","PeriodicalId":8235,"journal":{"name":"Annals of Animal Science","volume":"23 1","pages":"615 - 628"},"PeriodicalIF":1.9,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49229669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：