Archives of Medical Science最新文献

Introduction: The aim of the study was to observe the effect of Sanguiin on cerebral edema and behavior in a rat cerebral hemorrhage model.

Methods: A rat collagenase-induced cerebral hemorrhage model was established to detect the effects of drugs on brain edema.

Results: Through magnetic resonance imaging (MRI) analysis and brain weight content (BWC) determination, it was found that Sanguiin could significantly reduce the brain swelling index and BWC of the affected hemisphere after cerebral hemorrhage.

Conclusions: Sanguiin can significantly improve the neurological deficits in rats with cerebral hemorrhage, and down-regulate the expression of MMP-9 after cerebral hemorrhage, suggesting that Sanguiin has a certain protective effect on the blood-brain barrier after cerebral hemorrhage.

Introduction: Trace mineral micronutrients are essential for periodontal health, and unbalanced levels of trace minerals are related to chronic periodontitis, including copper. We attempted to explore the relationship between cuproptosis and periodontitis.

Methods: Bulk RNA-seq and single-cell RNA sequencing data were used to analyze expression of cuproptosis-related genes (CRGs) in periodontitis. A mouse periodontitis model was established to verify the expression of key genes.

Results: Cuproptosis-related genes were differentially expressed in periodontitis. RT-PCR results confirmed the alteration of five key genes in the mouse model.

Conclusions: Cuproptosis is an important player in the pathophysiological process of periodontitis.

Introduction: The association of air pollution with bone mineral density (BMD) has attracted increasing attention. However, establishing a causal relationship remains uncertain.

Methods: We conducted a Mendelian randomization (MR) study employing PM_2.5, PM_2.5-10, PM₁₀, nitrogen dioxide, and nitrogen oxides as exposures and BMD as the outcome to explore the causality between air pollution and the occurrence of decreased BMD.

Results: By employing the IVW method, we identified a negative causality between air pollution (PM_2.5, PM₁₀, and nitrogen oxides) and BMD.

Conclusions: Our findings demonstrate that PM_2.5, PM₁₀ and nitrogen oxides exposure may contribute to decreased BMD.

Introduction: The present study concerns a connection of the Q192RPON1 polymorphism with atherosclerosis requiring percutaneous coronary intervention (PCI) or coronary artery bypass grafting (CABG) in the Polish population.

Methods: A total of 282 individuals who underwent coronary angiography took part in this study. The polymorphism was determined with the PCR-RFLP method.

Results: The odds ratio for atherosclerosis in carriers of the 192RR genotype was 2.50 (p = 0.002). The median HDL-C concentration was significantly lower in the study group than the control group (p = 0.02).

Conclusions: The presence of the 192RR genotype and 192R allele are indicative of at least a two-fold increased risk of atherosclerosis requiring PCI or CABG in the Polish population.

Introduction: The study aimed to evaluate, for the first time, the diagnostic value of long non-coding RNA (lncRNA) small nucleolar RNA host gene 8 (SNHG8) in sepsis and its molecular mechanisms in sepsis-induced inflammation and cardiac dysfunction.

Material and methods: A total of 126 sepsis patients and 81 healthy controls were enrolled. Serum SNHG8 levels were assessed by RT-qPCR. Levels of pro-inflammatory factors were examined via ELISA. The ROC curve was employed to assess the diagnostic significance of SNHG8. Cardiomyocytes were exposed to lipopolysaccharide (LPS) to simulate sepsis-induced cardiac dysfunction in vitro. Cell proliferation and apoptosis were measured through CCK-8 and flow cytometry. Dual luciferase reporter gene assay and RIP assay were conducted to verify the target relationship between SNHG8 and miR-34b-5p.

Results: SNHG8 was reduced in sepsis patients (p < 0.05) and negatively correlated with procalcitonin, C-reactive protein, and pro-inflammatory factors (p < 0.05). SNHG8 had outstanding performance in distinguishing sepsis patients from healthy individuals with the AUC of 0.878. Among septic patients, those with cardiac dysfunction had significantly downregulated SNHG8 levels (p < 0.05). For septic patients, SNHG8 was found to be an independent predictor for the occurrence of cardiac dysfunction (HR = 5.466, 95% CI = 2.230-13.397, p < 0.001). Elevated SNHG8 reversed LPS-induced cell apoptosis, and attenuated the over-secretion of inflammatory factors. miR-34b-5p was significantly upregulated in septic patients and negatively correlated with SNHG8, indicating that it acted as a sponge for SNHG8.

Conclusions: Reduced SNHG8 is a potential diagnostic biomarker for sepsis. It is involved in sepsis-induced inflammatory response and cardiac dysfunction through sponging miR-34b-5p.

Introduction: The systemic immune-inflammation index (SII), based on peripheral lymphocyte, neutrophil, and platelet counts, has recently been investigated as a prognostic marker in several tumors. However, the SII has rarely been reported in skin cancers. In this study, we aimed to assess the association between SII values and the risk of occurrence of skin cancers.

Material and methods: This cross-sectional study was based on National Health and Nutrition Examination Survey data from 2010 to 2018 and involved 32,012 participants. The SII was calculated as the platelet count × neutrophil count/lymphocyte count. A weighted multivariate logistic analysis was conducted to examine the relationship between SII values and the occurrence of skin cancers. In addition, a subgroup analysis and a sensitivity analysis were conducted to identify underlying moderators and the stability of the relationship, respectively.

Results: Compared with participants in the lowest quartile of SII values, the odds ratios for non-melanoma skin cancer were 1.650 (95% CI: 1.158-2.352) for participants in the quartile with the highest SII values after multivariate adjustments. In subgroup analyses, we found significant interactions between log-transformed SII values and age (p < 0.001 for interaction), race (p < 0.001 for interaction), education level (p < 0.001 for interaction), marital status (p < 0.001 for interaction), and annual household incomes (p < 0.001 for interaction) in the association with non-melanoma skin cancer.

Conclusions: Our findings suggest a positive association between high SII values and skin cancers in the U.S. population. Age, levels of education, marital status, and annual household incomes affect the positive association between high SII values and non-melanoma skin cancers.