Given the significant interactions between hydrogen sulfide (H2S) and cysteine (Cys) in organisms, a dual-site multi-purpose fluorescent probe (Cy-NP) for H2S and Cys was synthesized. Cy-NP is composed of two fluorophores: naphthalimide that emits in the visible region of 500-600 nm, and cyanine dye that emits in the NIR region of 700-800 nm. Cy-NP showed admirable sensitivity and selectivity for identifying H2S and Cys by fluorescent signals with limits of detection as low as 0.15 μM and 1.4 μM, respectively. Furthermore, other biological thiols (especially GSH and Hcy) showed no positive response to Cy-NP compared with H2S and Cys. The chemical mechanism of Cy-NP with H2S and Cys in DMF/PBS (1/1, v/v, pH = 7.4) solution was verified by HRMS and DFT calculations. Further, Cy-NP was successfully applied to monitor H2S released in raw meat and adapted to detect H2S and Cys in MCF-7 cells independently and continuously.
{"title":"A dual-response NIR fluorescent probe for separately and continuously recognizing H2S and Cys with different fluorescence signals and its applications.","authors":"Lisha Yue, Yin Ai, Gang Liu, Haichang Ding, S. Pu","doi":"10.2139/ssrn.4331007","DOIUrl":"https://doi.org/10.2139/ssrn.4331007","url":null,"abstract":"Given the significant interactions between hydrogen sulfide (H2S) and cysteine (Cys) in organisms, a dual-site multi-purpose fluorescent probe (Cy-NP) for H2S and Cys was synthesized. Cy-NP is composed of two fluorophores: naphthalimide that emits in the visible region of 500-600 nm, and cyanine dye that emits in the NIR region of 700-800 nm. Cy-NP showed admirable sensitivity and selectivity for identifying H2S and Cys by fluorescent signals with limits of detection as low as 0.15 μM and 1.4 μM, respectively. Furthermore, other biological thiols (especially GSH and Hcy) showed no positive response to Cy-NP compared with H2S and Cys. The chemical mechanism of Cy-NP with H2S and Cys in DMF/PBS (1/1, v/v, pH = 7.4) solution was verified by HRMS and DFT calculations. Further, Cy-NP was successfully applied to monitor H2S released in raw meat and adapted to detect H2S and Cys in MCF-7 cells independently and continuously.","PeriodicalId":82956,"journal":{"name":"The Behavior analyst","volume":"82 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83745286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
He Xingliang, Guo Xing, Wu Mengfan, Deng Fulong, Zeng Pengyu, Z. Zhongjun, Duan Yixiang
In this study, we propose a novel ion formation simulation method for electrospray ionization (ESI) and atmosphere pressure interface (API). In this method, not the sheer particle trajectory, but the evolution of droplets and the offspring of gaseous ions are introduced instead. For the first time, the dynamic droplet-to-ion transformation process in the API of ESI-MS is visualized. The results suggest that this model provides a better understanding of the ion evolution mechanism and we propose a way for mass spectrometer structure optimization and ion source parameter adjustment in new aspects.
{"title":"From droplets to ions: a comprehensive and consecutive ion formation modelling in atmosphere pressure interface of electrospray ionization mass spectrometry.","authors":"He Xingliang, Guo Xing, Wu Mengfan, Deng Fulong, Zeng Pengyu, Z. Zhongjun, Duan Yixiang","doi":"10.2139/ssrn.4397243","DOIUrl":"https://doi.org/10.2139/ssrn.4397243","url":null,"abstract":"In this study, we propose a novel ion formation simulation method for electrospray ionization (ESI) and atmosphere pressure interface (API). In this method, not the sheer particle trajectory, but the evolution of droplets and the offspring of gaseous ions are introduced instead. For the first time, the dynamic droplet-to-ion transformation process in the API of ESI-MS is visualized. The results suggest that this model provides a better understanding of the ion evolution mechanism and we propose a way for mass spectrometer structure optimization and ion source parameter adjustment in new aspects.","PeriodicalId":82956,"journal":{"name":"The Behavior analyst","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74409686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rasmus Öberg, Tobias Dahlberg, Dmitry Malyshev, Magnus Andersson
Endospore-forming bacteria are associated with food spoilage, food poisoning, and infection in hospitals. Therefore, methods to monitor spore metabolic activity and verify sterilization are of great interest. However, current methods for tracking metabolic activity are time-consuming and resource intensive. This work investigates isotope labeling and Raman microscopy as a low-cost rapid alternative. Specifically, we monitor the Raman spectrum of enterotoxic B. cereus spores undergoing germination and cell division in D2O-infused broth. During germination and cell division, water is metabolized and deuterium from the broth is incorporated into proteins and lipids, resulting in the appearance of a Raman peak related to C-D bonds at 2190 cm-1. We find that a significant C-D peak appears after 2 h of incubation at 37 °C. Further, we found that the peak appearance coincides with the observed first cell division indicating little metabolic activity during germination. Lastly, the germination and cell growth rate of spores were not affected by adding 30% heavy water to the broth. This shows the potential for real-time monitoring of metabolic activity from a bacterial spore to a dividing cell. In conclusion, our work proposes tracking the evolution of the C-D Raman peak in spores incubated with D2O-infused broth as an effective and time-, and cost-efficient method to monitor the outgrowth of a spore population, simultaneously allowing us to track for how long the bacteria have grown and divided.
{"title":"Monitoring bacterial spore metabolic activity using heavy water-induced Raman peak evolution.","authors":"Rasmus Öberg, Tobias Dahlberg, Dmitry Malyshev, Magnus Andersson","doi":"10.2139/ssrn.4292992","DOIUrl":"https://doi.org/10.2139/ssrn.4292992","url":null,"abstract":"Endospore-forming bacteria are associated with food spoilage, food poisoning, and infection in hospitals. Therefore, methods to monitor spore metabolic activity and verify sterilization are of great interest. However, current methods for tracking metabolic activity are time-consuming and resource intensive. This work investigates isotope labeling and Raman microscopy as a low-cost rapid alternative. Specifically, we monitor the Raman spectrum of enterotoxic B. cereus spores undergoing germination and cell division in D2O-infused broth. During germination and cell division, water is metabolized and deuterium from the broth is incorporated into proteins and lipids, resulting in the appearance of a Raman peak related to C-D bonds at 2190 cm-1. We find that a significant C-D peak appears after 2 h of incubation at 37 °C. Further, we found that the peak appearance coincides with the observed first cell division indicating little metabolic activity during germination. Lastly, the germination and cell growth rate of spores were not affected by adding 30% heavy water to the broth. This shows the potential for real-time monitoring of metabolic activity from a bacterial spore to a dividing cell. In conclusion, our work proposes tracking the evolution of the C-D Raman peak in spores incubated with D2O-infused broth as an effective and time-, and cost-efficient method to monitor the outgrowth of a spore population, simultaneously allowing us to track for how long the bacteria have grown and divided.","PeriodicalId":82956,"journal":{"name":"The Behavior analyst","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84605017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yafei Li, Yang Lu, Chuantao Zheng, Shuo Yang, Kaiyuan Zheng, Fang Song, Chunguang Li, W. Ye, Yu Zhang, Yiding Wang, F. Tittel
To realize early fire identification in cotton harvesting operations, a mid-infrared carbon monoxide (CO) sensor system was developed. To match the broadband light source with a 15° divergence angle, a multipass gas cell (MPGC) with an effective path length of 180 cm was designed to improve sensor sensitivity, leading to a limit of detection (LoD) of 0.83 parts-per-million by volume (ppmv). A damping module with springs at the bottom and front/back sides was fabricated, which can effectively reduce the vibration intensity by >80%. The sensor system can operate normally from -40 °C to 85 °C by stabilizing the temperature of the optical module through heating or cooling as well as using automotive electronic components. An adaptive early fire identification algorithm based on a dual-parameter threshold alarming method was proposed to avoid false and missing alarms. Field deployments on a harvester verified the good practicability of the sensor system.
{"title":"Development of a mid-infrared sensor system for early fire identification in cotton harvesting operations.","authors":"Yafei Li, Yang Lu, Chuantao Zheng, Shuo Yang, Kaiyuan Zheng, Fang Song, Chunguang Li, W. Ye, Yu Zhang, Yiding Wang, F. Tittel","doi":"10.2139/ssrn.4167287","DOIUrl":"https://doi.org/10.2139/ssrn.4167287","url":null,"abstract":"To realize early fire identification in cotton harvesting operations, a mid-infrared carbon monoxide (CO) sensor system was developed. To match the broadband light source with a 15° divergence angle, a multipass gas cell (MPGC) with an effective path length of 180 cm was designed to improve sensor sensitivity, leading to a limit of detection (LoD) of 0.83 parts-per-million by volume (ppmv). A damping module with springs at the bottom and front/back sides was fabricated, which can effectively reduce the vibration intensity by >80%. The sensor system can operate normally from -40 °C to 85 °C by stabilizing the temperature of the optical module through heating or cooling as well as using automotive electronic components. An adaptive early fire identification algorithm based on a dual-parameter threshold alarming method was proposed to avoid false and missing alarms. Field deployments on a harvester verified the good practicability of the sensor system.","PeriodicalId":82956,"journal":{"name":"The Behavior analyst","volume":"70 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77539161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mengjiao Dai, Qunyan Zhu, Dongxue Han, L. Niu, Zhenxin Wang
The hydrogen peroxide (H2O2) levels in living organisms and environment have strong effects on many biological processes inducing cell apoptosis/cell necrosis and wound disinfection. Therefore, it is important to have an accurate and in situ detection of H2O2. Herein, an AuPd@FexOy nanozyme-based electrochemical (EC) sensor (termed as AuPd@FexOy NPs/GCE) with good stability and anti-interference ability has been prepared for the detection of H2O2 by differential pulse voltammetry (DPV) and chronoamperometry dual-measurement modes. The AuPd@FexOy NPs/GCE exhibits good linear relationships in the ranges from 13.0 to 6.0 × 103 μM (DPV measurement) and 50 to 1.0 × 103 μM (chronoamperometry measurement), low detection limits (LODs) of 1.6 μM (DPV measurement) and 3.0 μM (chronoamperometry measurement) and high sensitivities of 83.8 nA μM-1 cm-2 (DPV measurement) and 120.7 nA μM-1 cm-2 (chronoamperometry measurement). The practicability of the as-prepared AuPd@FexOy NPs/GCE has been demonstrated by an in situ real-time detection of H2O2 released from adherent living MCF-7 cells triggered by varying amounts of N-formyl-L-methionyl-L-leucyl-L-phenylalanine (FMLP) from 0.5 to 3.0 μM and the quantitative determination of H2O2 in commercial disinfectants.
{"title":"An electrochemical sensor based on AuPd@FexOy nanozymes for a sensitive and in situ quantitative detection of hydrogen peroxide in real samples.","authors":"Mengjiao Dai, Qunyan Zhu, Dongxue Han, L. Niu, Zhenxin Wang","doi":"10.2139/ssrn.4165889","DOIUrl":"https://doi.org/10.2139/ssrn.4165889","url":null,"abstract":"The hydrogen peroxide (H2O2) levels in living organisms and environment have strong effects on many biological processes inducing cell apoptosis/cell necrosis and wound disinfection. Therefore, it is important to have an accurate and in situ detection of H2O2. Herein, an AuPd@FexOy nanozyme-based electrochemical (EC) sensor (termed as AuPd@FexOy NPs/GCE) with good stability and anti-interference ability has been prepared for the detection of H2O2 by differential pulse voltammetry (DPV) and chronoamperometry dual-measurement modes. The AuPd@FexOy NPs/GCE exhibits good linear relationships in the ranges from 13.0 to 6.0 × 103 μM (DPV measurement) and 50 to 1.0 × 103 μM (chronoamperometry measurement), low detection limits (LODs) of 1.6 μM (DPV measurement) and 3.0 μM (chronoamperometry measurement) and high sensitivities of 83.8 nA μM-1 cm-2 (DPV measurement) and 120.7 nA μM-1 cm-2 (chronoamperometry measurement). The practicability of the as-prepared AuPd@FexOy NPs/GCE has been demonstrated by an in situ real-time detection of H2O2 released from adherent living MCF-7 cells triggered by varying amounts of N-formyl-L-methionyl-L-leucyl-L-phenylalanine (FMLP) from 0.5 to 3.0 μM and the quantitative determination of H2O2 in commercial disinfectants.","PeriodicalId":82956,"journal":{"name":"The Behavior analyst","volume":"2002 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78554022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cuicui Fu, Li Zhang, Meiqi Bao, Yue Zhang, Ya-li Li, Yan Wu, Young Mee Jung
A novel surface-enhanced Raman scattering (SERS) immunoassay method based on tyramine signal amplification (TSA) technology triggering the formation of enzyme repeats on an enzyme-linked immunosorbent assay (ELISA) was designed for highly sensitive detection of human chorionic gonadotropin (hCG) using enzymatic biocatalytic precipitation toward o-phenylenediamine (OPD). Initially, a horseradish peroxidase (HRP)-labeled hCG antibody was fixed by the double antibody sandwich method, and then a tyramine-HRP conjugate was used to form HRP repeats by triggering the immobilized HRP on ELISA with the aid of H2O2. In the presence of the target hCG, the HRP repeats carried by the sandwich immune complex catalyzed the oxidation of OPD to produce product molecules with different structures, resulting in changes in the SERS fingerprint spectrum. The analytical performance of the SERS immunoassay was studied in detail using SERS spectral characterization. Under the optimum conditions, the immunosensor displayed a working range from 1 IU L-1 to 16 IU L-1 with a detection limit (LOD) of 0.17 IU L-1 relative to the target hCG. Compared to the traditional SERS immunosensor, a higher detection sensitivity can be obtained. Therefore, this work provides a new strategy for hCG detection and inspiration for the construction of sensitive and efficient immunosensors.
设计了一种基于酪胺信号放大(TSA)技术的表面增强拉曼散射(SERS)免疫分析方法，该方法在酶联免疫吸附试验(ELISA)上触发酶重复序列的形成，用于酶促生物催化沉淀对邻苯二胺(OPD)的高灵敏度检测人绒毛膜促性腺激素(hCG)。首先用双抗体夹心法固定辣根过氧化物酶(HRP)标记的hCG抗体，然后用酪胺-HRP偶联物在ELISA上用H2O2触发固定的HRP形成HRP重复序列。在靶hCG存在的情况下，夹心免疫复合体携带的HRP重复序列催化OPD氧化产生不同结构的产物分子，导致SERS指纹图谱发生变化。利用SERS光谱表征对SERS免疫分析法的分析性能进行了详细的研究。在最佳条件下，免疫传感器的工作范围为1 ~ 16 IU L-1，相对于目标hCG的检出限为0.17 IU L-1。与传统的SERS免疫传感器相比，可以获得更高的检测灵敏度。因此，本工作为hCG检测提供了新的策略，并为构建灵敏高效的免疫传感器提供了启示。
{"title":"Signal amplification surface-enhanced Raman scattering immunosorbent assay of human chorionic gonadotrophin based on repeated enzyme biocatalytic precipitation.","authors":"Cuicui Fu, Li Zhang, Meiqi Bao, Yue Zhang, Ya-li Li, Yan Wu, Young Mee Jung","doi":"10.2139/ssrn.4197044","DOIUrl":"https://doi.org/10.2139/ssrn.4197044","url":null,"abstract":"A novel surface-enhanced Raman scattering (SERS) immunoassay method based on tyramine signal amplification (TSA) technology triggering the formation of enzyme repeats on an enzyme-linked immunosorbent assay (ELISA) was designed for highly sensitive detection of human chorionic gonadotropin (hCG) using enzymatic biocatalytic precipitation toward o-phenylenediamine (OPD). Initially, a horseradish peroxidase (HRP)-labeled hCG antibody was fixed by the double antibody sandwich method, and then a tyramine-HRP conjugate was used to form HRP repeats by triggering the immobilized HRP on ELISA with the aid of H2O2. In the presence of the target hCG, the HRP repeats carried by the sandwich immune complex catalyzed the oxidation of OPD to produce product molecules with different structures, resulting in changes in the SERS fingerprint spectrum. The analytical performance of the SERS immunoassay was studied in detail using SERS spectral characterization. Under the optimum conditions, the immunosensor displayed a working range from 1 IU L-1 to 16 IU L-1 with a detection limit (LOD) of 0.17 IU L-1 relative to the target hCG. Compared to the traditional SERS immunosensor, a higher detection sensitivity can be obtained. Therefore, this work provides a new strategy for hCG detection and inspiration for the construction of sensitive and efficient immunosensors.","PeriodicalId":82956,"journal":{"name":"The Behavior analyst","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91174063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. G. Gurudatt, Kyungyeon Lee, W. Heo, Hyo-Il Jung
Rapid detection of contaminants for the purpose of sensitive and quantitative monitoring of environmental hazards is an essential first step in realizing the avoidance of human health risks. In this regard, we present a fast and simple electrochemical method of detecting di-n-butyl phthalate (DBP) from river water samples using a phthalic acid group specific aptamer modified on a gold nanoparticle (AuNP) functionalized graphene oxide nano-platelet (GO) and ionic liquid (IL) nanocomposite. Here, the IL/GO nanocomposite allows an enhanced interaction with phthalate esters, thereby increasing the sensitivity of the sensor surface. The proposed sensor showed a wide linear dynamic range from 0.14 pg mL-1 to 0.35 ng mL-1 and from 0.35 ng mL-1 to 7 ng mL-1 with a detection limit of ≤0.042 pg mL-1, which were evaluated using standard, analytical grade DBP; the limit of quantification was determined using different concentrations of DBP in DI water in comparison with gas chromatography-mass spectroscopy (GC/MS) values. The proposed sensor was used to monitor the DBP concentrations in river water samples collected from various locations across South Korea. The quantitative data from the measurements in comparison with standard GC/MS values were then used to ascertain the human health risk posed by the daily consumption of these river waters.
快速检测污染物，以便对环境危害进行敏感和定量监测，是实现避免人类健康风险的重要第一步。在这方面，我们提出了一种快速、简单的电化学方法，利用在金纳米粒子(AuNP)功能化氧化石墨烯纳米板(GO)和离子液体(IL)纳米复合材料上修饰的邻苯二甲酸基特异性适配体，从河流水样中检测邻苯二甲酸二丁酯(DBP)。在这里，IL/GO纳米复合材料允许增强与邻苯二甲酸酯的相互作用，从而提高传感器表面的灵敏度。该传感器的线性动态范围为0.14 ~ 0.35 ng mL-1和0.35 ~ 7 ng mL-1，检测限≤0.042 pg mL-1，采用标准分析级DBP进行评价;通过气相色谱-质谱(GC/MS)分析，确定了去水中不同浓度DBP的定量限。该传感器被用于监测从韩国各地收集的河流水样中的DBP浓度。测量所得的定量数据与标准气相色谱/质谱值进行比较，然后用于确定每日饮用这些河水对人体健康构成的风险。
{"title":"Simple ultrasensitive electrochemical detection of the DBP plasticizer for the risk assessment of South Korean river waters.","authors":"N. G. Gurudatt, Kyungyeon Lee, W. Heo, Hyo-Il Jung","doi":"10.2139/ssrn.4069170","DOIUrl":"https://doi.org/10.2139/ssrn.4069170","url":null,"abstract":"Rapid detection of contaminants for the purpose of sensitive and quantitative monitoring of environmental hazards is an essential first step in realizing the avoidance of human health risks. In this regard, we present a fast and simple electrochemical method of detecting di-n-butyl phthalate (DBP) from river water samples using a phthalic acid group specific aptamer modified on a gold nanoparticle (AuNP) functionalized graphene oxide nano-platelet (GO) and ionic liquid (IL) nanocomposite. Here, the IL/GO nanocomposite allows an enhanced interaction with phthalate esters, thereby increasing the sensitivity of the sensor surface. The proposed sensor showed a wide linear dynamic range from 0.14 pg mL-1 to 0.35 ng mL-1 and from 0.35 ng mL-1 to 7 ng mL-1 with a detection limit of ≤0.042 pg mL-1, which were evaluated using standard, analytical grade DBP; the limit of quantification was determined using different concentrations of DBP in DI water in comparison with gas chromatography-mass spectroscopy (GC/MS) values. The proposed sensor was used to monitor the DBP concentrations in river water samples collected from various locations across South Korea. The quantitative data from the measurements in comparison with standard GC/MS values were then used to ascertain the human health risk posed by the daily consumption of these river waters.","PeriodicalId":82956,"journal":{"name":"The Behavior analyst","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83261140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The development of portable solid-phase biosensors is of great significance for point-of-care testing (POCT). In this work, we constructed a simple visualized solid-phase biosensor based on luminescence resonance energy transfer (LRET) from upconversion nanoparticles (UCNPs) to gold nanoparticles (AuNPs) for quantitative detection of virus-specific nucleic acid sequences. The detection data showed that there was a linear relationship between the luminescence recovery of UCNPs and the concentrations of the target within the range of 5-100 nM, and the limit of detection (LOD) was 0.326 nM. Additionally, the luminescence recovery of UCNPs was visualized and quantitatively analyzed using a home-built luminescence image capture device and an open-source ImageJ software that can analyze and process images. Compared with conventional liquid-phase biosensors, the solid-phase analysis method we constructed not only has advantages in cost, portability and stability, but also is more conducive to the rapid acquisition and storage of the detected sample, which is expected to become a fast, efficient and reliable detection platform for POCT.
{"title":"A facile visualized solid-phase detection of virus-specific nucleic acid sequences through an upconversion activated linear luminescence recovery process.","authors":"Xiaorong Liu, Chaonan He, Qi Huang, Mengmeng Yu, Zhuang Qiu, Haoxin Cheng, Yifei Yang, Xian Hao, Xiaolei Wang","doi":"10.2139/ssrn.4015241","DOIUrl":"https://doi.org/10.2139/ssrn.4015241","url":null,"abstract":"The development of portable solid-phase biosensors is of great significance for point-of-care testing (POCT). In this work, we constructed a simple visualized solid-phase biosensor based on luminescence resonance energy transfer (LRET) from upconversion nanoparticles (UCNPs) to gold nanoparticles (AuNPs) for quantitative detection of virus-specific nucleic acid sequences. The detection data showed that there was a linear relationship between the luminescence recovery of UCNPs and the concentrations of the target within the range of 5-100 nM, and the limit of detection (LOD) was 0.326 nM. Additionally, the luminescence recovery of UCNPs was visualized and quantitatively analyzed using a home-built luminescence image capture device and an open-source ImageJ software that can analyze and process images. Compared with conventional liquid-phase biosensors, the solid-phase analysis method we constructed not only has advantages in cost, portability and stability, but also is more conducive to the rapid acquisition and storage of the detected sample, which is expected to become a fast, efficient and reliable detection platform for POCT.","PeriodicalId":82956,"journal":{"name":"The Behavior analyst","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88066556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xiaochong Song, Siyu Luo, Jun Liu, Yuanfei Wu, Xiaojia Huang
Efficient separation and enrichment is a crucial step in the analysis of Se(IV) and Se(VI). In the present study, for the first time, online monolith-based magnetic field-assisted in-tube solid phase microextraction (MFA/IT-SPME) was applied to capture inorganic selenium species in water samples. To this aim, porous monoliths mixed with magnetic nanoparticles were synthesized in a silica capillary and employed as a microextraction column (MEC) for MFA/IT-SPME. After that, a magnetic coil utilized to induce variable magnetic fields in adsorption and desorption steps was entwined around the MEC. Se(IV) was coordinated with o-phenylenediamine to form a coordination compound that was infused onto the MEC to be captured. Results evidenced that application of magnetic field during the extraction procedure assisted the capture of the Se(IV)-OPA complex, with an enhancement in the extraction efficiency from 83% to 97%. Under the optimized conditions, MFA/IT-SPME was online combined with HPLC equipped with a diode array detector (DAD) to perform quantification of Se(IV) and Se(VI) in environmental water samples. Total inorganic Se was quantified after pre-reduction of Se(VI) to Se(IV) prior to applying the established approach, and a subtraction method was adopted to calculate the Se(VI) and Se(IV) contents. The limit of detection for Se(IV) was as low as 0.012 μg L-1. The reliability of the suggested method was investigated by assaying Se(IV) and Se(VI) species in real-life water samples with satisfactory recoveries (81.1%-116%) and repeatability (RSDs below 9%).
{"title":"Fabrication of functional group-rich monoliths for magnetic field-assisted in-tube solid phase microextraction of inorganic selenium species in water samples followed by online chromatographic determination.","authors":"Xiaochong Song, Siyu Luo, Jun Liu, Yuanfei Wu, Xiaojia Huang","doi":"10.2139/ssrn.3946284","DOIUrl":"https://doi.org/10.2139/ssrn.3946284","url":null,"abstract":"Efficient separation and enrichment is a crucial step in the analysis of Se(IV) and Se(VI). In the present study, for the first time, online monolith-based magnetic field-assisted in-tube solid phase microextraction (MFA/IT-SPME) was applied to capture inorganic selenium species in water samples. To this aim, porous monoliths mixed with magnetic nanoparticles were synthesized in a silica capillary and employed as a microextraction column (MEC) for MFA/IT-SPME. After that, a magnetic coil utilized to induce variable magnetic fields in adsorption and desorption steps was entwined around the MEC. Se(IV) was coordinated with o-phenylenediamine to form a coordination compound that was infused onto the MEC to be captured. Results evidenced that application of magnetic field during the extraction procedure assisted the capture of the Se(IV)-OPA complex, with an enhancement in the extraction efficiency from 83% to 97%. Under the optimized conditions, MFA/IT-SPME was online combined with HPLC equipped with a diode array detector (DAD) to perform quantification of Se(IV) and Se(VI) in environmental water samples. Total inorganic Se was quantified after pre-reduction of Se(VI) to Se(IV) prior to applying the established approach, and a subtraction method was adopted to calculate the Se(VI) and Se(IV) contents. The limit of detection for Se(IV) was as low as 0.012 μg L-1. The reliability of the suggested method was investigated by assaying Se(IV) and Se(VI) species in real-life water samples with satisfactory recoveries (81.1%-116%) and repeatability (RSDs below 9%).","PeriodicalId":82956,"journal":{"name":"The Behavior analyst","volume":"86 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83971194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-02-10DOI: 10.26434/chemrxiv-2022-rmf6x
Stephanie Rankin-Turner, C. McMeniman
The human body secretes a complex blend of volatile organic compounds (VOCs) via the skin, breath and bodily fluids, the study of which can provide valuable insight into the physiological and metabolic state of an individual. Methods to profile human-derived volatiles typically source VOCs from bodily fluids, exhaled breath or skin of isolated body parts. To facilitate profiling the whole body volatilome, we have engineered a sampling chamber that enables the collection and analysis of headspace from the entire human body. Whole body VOCs were collected from a cohort of 20 humans and analyzed by thermal desorption-gas chromatography/mass spectrometry (TD-GC/MS) to characterize the compounds present in whole body headspace and evaluate chemical differences between individuals. A range of compounds were detected and identified in whole body headspace including ketones, carboxylic acids, aldehydes, alcohols, and aliphatic and aromatic hydrocarbons. Considerable heterogeneity in the chemical composition of whole body odor and the concentration of its constituent compounds was observed across individuals. Amongst the most common and abundant compounds detected in human whole body odor were sulcatone, acetoin, acetic acid and C6-C10 aldehydes. This method facilitates standardized and quantitative analytical profiling of the human whole body volatilome.
{"title":"A headspace collection chamber for whole body volatilomics.","authors":"Stephanie Rankin-Turner, C. McMeniman","doi":"10.26434/chemrxiv-2022-rmf6x","DOIUrl":"https://doi.org/10.26434/chemrxiv-2022-rmf6x","url":null,"abstract":"The human body secretes a complex blend of volatile organic compounds (VOCs) via the skin, breath and bodily fluids, the study of which can provide valuable insight into the physiological and metabolic state of an individual. Methods to profile human-derived volatiles typically source VOCs from bodily fluids, exhaled breath or skin of isolated body parts. To facilitate profiling the whole body volatilome, we have engineered a sampling chamber that enables the collection and analysis of headspace from the entire human body. Whole body VOCs were collected from a cohort of 20 humans and analyzed by thermal desorption-gas chromatography/mass spectrometry (TD-GC/MS) to characterize the compounds present in whole body headspace and evaluate chemical differences between individuals. A range of compounds were detected and identified in whole body headspace including ketones, carboxylic acids, aldehydes, alcohols, and aliphatic and aromatic hydrocarbons. Considerable heterogeneity in the chemical composition of whole body odor and the concentration of its constituent compounds was observed across individuals. Amongst the most common and abundant compounds detected in human whole body odor were sulcatone, acetoin, acetic acid and C6-C10 aldehydes. This method facilitates standardized and quantitative analytical profiling of the human whole body volatilome.","PeriodicalId":82956,"journal":{"name":"The Behavior analyst","volume":"63 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80784460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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