Sonny C Ramos, Pamela Denise B Dosono, Ma Carol Anne C Untalasco, Mila Rose M Villamin, Jocelyn R Rafanan, C. Mingala
Alpha (1, 2)-fucosyltransferase (FUT1), as a candidate gene in controlling the expression of E. coli F18 receptor has been identified to determine whether an animal is resistant or susceptible to infections. This study was conducted to determine the genotypes of 150 blood samples of the three swine breeds and 20 representatives were randomly selected for sequencing. Polymerase Chain Reaction - Restriction Fragment Length Polymorphism results revealed that among the 150 blood samples, seven of the Duroc x Pietrain samples carried AG genotype which was previously reported susceptible to ETEC infection. Two AA genotypes that are presumptively resistant and 50 were presumptively susceptible as these samples carried either AG or GG genotypes from the Landrace variety. Of the Large White samples, two samples carried AA genotype and 89 have either AG or GG genotype. The twenty samples sent for DNA sequencing registered 100% homologies to Sus scrofa FUT1 gene. Twelve of the sequenced samples exhibited a shift from G to A in the 117th nucleotide and one sample had a C to T shift in the 39th nucleotide. The mutation was found in all of the Duroc X Pietrain samples as well as those samples with AA genotype. Heterozygous forms of Landrace and Large White also exhibited this mutation.
α（1，2）-岩藻糖基转移酶（FUT1）作为控制大肠杆菌 F18 受体表达的候选基因，已被确定用于确定动物对感染的抵抗力还是易感性。本研究测定了三个猪种的 150 份血液样本的基因型，并随机抽取了 20 份样本进行测序。聚合酶链式反应--限制性片段长度多态性结果显示，在 150 份血液样本中，有 7 份杜洛克 x 皮特兰样本携带 AG 基因型，而据先前报道，AG 基因型对 ETEC 感染易感。两个 AA 基因型被推定为具有抗性，50 个被推定为易感，因为这些样本携带的是来自兰德瑞品种的 AG 或 GG 基因型。在大白样本中，两个样本带有 AA 基因型，89 个样本带有 AG 或 GG 基因型。送去进行 DNA 测序的 20 个样本与褐马鸡 FUT1 基因的同源性为 100%。其中 12 个测序样本的第 117 个核苷酸由 G 转变为 A，1 个样本的第 39 个核苷酸由 C 转变为 T。在所有杜洛克 X 皮特兰样本和 AA 基因型样本中都发现了这种突变。杂合型的兰德瑞斯和大白也出现了这种突变。
{"title":"Genotyping and Molecular Detection of Polymorphism in FUT1 Gene of Swine","authors":"Sonny C Ramos, Pamela Denise B Dosono, Ma Carol Anne C Untalasco, Mila Rose M Villamin, Jocelyn R Rafanan, C. Mingala","doi":"10.5380/avs.v28i3.89326","DOIUrl":"https://doi.org/10.5380/avs.v28i3.89326","url":null,"abstract":"Alpha (1, 2)-fucosyltransferase (FUT1), as a candidate gene in controlling the expression of E. coli F18 receptor has been identified to determine whether an animal is resistant or susceptible to infections. This study was conducted to determine the genotypes of 150 blood samples of the three swine breeds and 20 representatives were randomly selected for sequencing. Polymerase Chain Reaction - Restriction Fragment Length Polymorphism results revealed that among the 150 blood samples, seven of the Duroc x Pietrain samples carried AG genotype which was previously reported susceptible to ETEC infection. Two AA genotypes that are presumptively resistant and 50 were presumptively susceptible as these samples carried either AG or GG genotypes from the Landrace variety. Of the Large White samples, two samples carried AA genotype and 89 have either AG or GG genotype. The twenty samples sent for DNA sequencing registered 100% homologies to Sus scrofa FUT1 gene. Twelve of the sequenced samples exhibited a shift from G to A in the 117th nucleotide and one sample had a C to T shift in the 39th nucleotide. The mutation was found in all of the Duroc X Pietrain samples as well as those samples with AA genotype. Heterozygous forms of Landrace and Large White also exhibited this mutation.","PeriodicalId":8351,"journal":{"name":"Archives of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139323387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Carlos Alailson Licar Rodrigues, Almerinda Macieira Medeiros, L. Tchaicka, L. P. F. ARAÚJO CHAVES, Soraia Alves Buarque, Alana Lislea DE SOUSA
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{"title":"Aspectos biométricos de ovos e embriões da tartaruga amazônica (Kinosternon scorpioides - Testudines: Kinosternidae) acompanhados em incubatório com temperatura controlada","authors":"Carlos Alailson Licar Rodrigues, Almerinda Macieira Medeiros, L. Tchaicka, L. P. F. ARAÚJO CHAVES, Soraia Alves Buarque, Alana Lislea DE SOUSA","doi":"10.5380/avs.v28i3.90028","DOIUrl":"https://doi.org/10.5380/avs.v28i3.90028","url":null,"abstract":".","PeriodicalId":8351,"journal":{"name":"Archives of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139324029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Glaucia Aparecida Aschenbrenner, R. Weiss, T. G. Bergstein-Galan, Fernando Andrade Souza, Natália Santana Siqueira De Lara, Vanessa Balan Julio
Increased rates of embryonic re-expansion and implantation have been reported following the use of forskolin during embryo culture, primarily attributed to the reduction of intraplasmic lipids, which improves cryopreservation. The aim of this meta-analysis was to compare the occurrence of embryonic re-expansion among different studies that utilized forskolin in vitro for embryo production. Five articles, out of 159, assessing forskolin at concentrations of 2.5, 5.0, and/or 10µM in embryo culture were considered from 1980 to 2022, comparing them to the control group (in vitro culture with forskolin). The Restricted Maximum Likelihood Method (REML) was employed to compare the results of the articles. The Q test was used to identify heterogeneity among the studies, and the I² analysis was used to quantify the heterogeneity between the studies and to quantify the heterogeneity between the studies. Based on the statistical analysis, it is inferred that embryos cultivated with forskolin at a concentration of 10µM are 71% more likely to re-expand, compared to the control group, with a 95% confidence interval, ranging from 27 to 132%. There was no statistically significant difference in the likelihood of embryonic re-expansion when comparing embryos treated with a forskolin at 5µM, and the control group, suggesting that a concentration of 10µM would enhance the quality of cryopreserved bovine embryos. Further experiments are required to define the correct concentration of forskolin in vitro for bovine embryos.
{"title":"META-ANALYSIS OF THE ACTION OF FORSKOLIN DURING IN VITRO CULTURE OF BOVINE EMBRYOS AND ITS IMPLICATION ON CRYOPRESERVATION","authors":"Glaucia Aparecida Aschenbrenner, R. Weiss, T. G. Bergstein-Galan, Fernando Andrade Souza, Natália Santana Siqueira De Lara, Vanessa Balan Julio","doi":"10.5380/avs.v28i3.90495","DOIUrl":"https://doi.org/10.5380/avs.v28i3.90495","url":null,"abstract":"Increased rates of embryonic re-expansion and implantation have been reported following the use of forskolin during embryo culture, primarily attributed to the reduction of intraplasmic lipids, which improves cryopreservation. The aim of this meta-analysis was to compare the occurrence of embryonic re-expansion among different studies that utilized forskolin in vitro for embryo production. Five articles, out of 159, assessing forskolin at concentrations of 2.5, 5.0, and/or 10µM in embryo culture were considered from 1980 to 2022, comparing them to the control group (in vitro culture with forskolin). The Restricted Maximum Likelihood Method (REML) was employed to compare the results of the articles. The Q test was used to identify heterogeneity among the studies, and the I² analysis was used to quantify the heterogeneity between the studies and to quantify the heterogeneity between the studies. Based on the statistical analysis, it is inferred that embryos cultivated with forskolin at a concentration of 10µM are 71% more likely to re-expand, compared to the control group, with a 95% confidence interval, ranging from 27 to 132%. There was no statistically significant difference in the likelihood of embryonic re-expansion when comparing embryos treated with a forskolin at 5µM, and the control group, suggesting that a concentration of 10µM would enhance the quality of cryopreserved bovine embryos. Further experiments are required to define the correct concentration of forskolin in vitro for bovine embryos.","PeriodicalId":8351,"journal":{"name":"Archives of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139323918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. H. Kifouly, Michael Okunlola, C. Boko, G. Alowanou, P. Challaton
Chlamydia abortus is a causative agent of Ovine Chlamydiosis or Ovine Enzootic Abortion (OEA) or Enzootic Abortion of Ewes (EAE) and can be transmitted to humans especially pregnant women during the lambing or kidding season, sheep, and goats from infected flocks represent a potential risk to pregnant women. Purpose: The objective of this study was to estimate the pooled prevalence of chlamydial abortus infections in small ruminants. Materials and Methods: The study followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Relevant studies were retrieved from PubMed, Scopus, Web of Science, and Google Scholar. The retrieved studies were screened for eligibility. Then, important data were extracted from the included studies. The quality of each included study was evaluated. Results: Of 145 studies, 25 (with a total of 15663 samples) met the inclusion criteria and were included in the meta-analysis. The pooled prevalence of chlamydial infections in small ruminants was 14,46 % (95%, CI: 14,07±15,18). Among continents, the average prevalence of chlamydial abortus infections was highest in Asia (52%) (95% CI: 32,42±71,58) and lowest in North America (4%) (95% CI: 3,68±11,68). Conclusions: Few studies have reported the prevalence of chlamydia infections in small ruminants. Based on the available data, the prevalence of chlamydia infections in small ruminants was quite high (14.46%). Therefore, small ruminants could be potential reservoirs of Chlamydia and pose a risk to humans especially pregnant women, or other animal species, especially in the wild.
流产衣原体是绵羊衣原体病或绵羊非典型流产（OEA）或母羊非典型流产（EAE）的病原体，可在产羔或产仔季节传播给人类，尤其是孕妇，来自受感染羊群的绵羊和山羊对孕妇构成潜在风险。目的：本研究旨在估算小反刍动物感染流产衣原体的总体流行率。材料与方法：本研究遵循系统综述和荟萃分析首选报告项目（PRISMA）指南。从 PubMed、Scopus、Web of Science 和 Google Scholar 检索相关研究。对检索到的研究进行资格筛选。然后，从纳入的研究中提取重要数据。对每项纳入研究的质量进行评估。结果：在 145 项研究中，有 25 项（共 15663 个样本）符合纳入标准并被纳入荟萃分析。小反刍动物衣原体感染的总体流行率为 14.46%（95%，CI：14.07±15.18）。在各大洲中，亚洲流产衣原体感染的平均流行率最高（52%）（95% CI：32,42±71,58），北美洲最低（4%）（95% CI：3,68±11,68）。结论很少有研究报告小型反刍动物的衣原体感染率。根据现有数据，小型反刍动物的衣原体感染率相当高（14.46%）。因此，小型反刍动物可能是衣原体的潜在储存库，对人类（尤其是孕妇）或其他动物物种（尤其是野生动物）构成风险。
{"title":"Ovine enzootic abortion disease seroprevalence in small ruminants around the world: a systematic review","authors":"A. H. Kifouly, Michael Okunlola, C. Boko, G. Alowanou, P. Challaton","doi":"10.5380/avs.v28i3.90853","DOIUrl":"https://doi.org/10.5380/avs.v28i3.90853","url":null,"abstract":"Chlamydia abortus is a causative agent of Ovine Chlamydiosis or Ovine Enzootic Abortion (OEA) or Enzootic Abortion of Ewes (EAE) and can be transmitted to humans especially pregnant women during the lambing or kidding season, sheep, and goats from infected flocks represent a potential risk to pregnant women. Purpose: The objective of this study was to estimate the pooled prevalence of chlamydial abortus infections in small ruminants. Materials and Methods: The study followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Relevant studies were retrieved from PubMed, Scopus, Web of Science, and Google Scholar. The retrieved studies were screened for eligibility. Then, important data were extracted from the included studies. The quality of each included study was evaluated. Results: Of 145 studies, 25 (with a total of 15663 samples) met the inclusion criteria and were included in the meta-analysis. The pooled prevalence of chlamydial infections in small ruminants was 14,46 % (95%, CI: 14,07±15,18). Among continents, the average prevalence of chlamydial abortus infections was highest in Asia (52%) (95% CI: 32,42±71,58) and lowest in North America (4%) (95% CI: 3,68±11,68). Conclusions: Few studies have reported the prevalence of chlamydia infections in small ruminants. Based on the available data, the prevalence of chlamydia infections in small ruminants was quite high (14.46%). Therefore, small ruminants could be potential reservoirs of Chlamydia and pose a risk to humans especially pregnant women, or other animal species, especially in the wild.","PeriodicalId":8351,"journal":{"name":"Archives of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139323962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
André Saldanha, Rodrigo Girata Machado, Barbara Decker Fernandes, Júlia Caroline de Oliveira, Gabriela Amorim Carvalho, Tatiane Brandão Moreno, C. Rocha
Psittacines are popular pet birds throughout the world. In the wild these birds consume a varied diet composed of seeds, nuts, flowers and invertebrates, according to seasonal availability. It is rare for captive birds to have a similar lifestyle and such a diverse diet. In captivity, mixed feeds (seeds and extruded feed) are available on the petfood market. However, these mixed diets allow birds to actively eat seeds resulting in non-balanced diets. The aim of this study was to determine selective voluntary intake of different diets based on different types of seeds and extruded feed in three different small/medium psittacine species commonly maintained as pets. Rose-ringed parakeets (P. krameri) preferentially consumed sunflower seeds, while lovebirds (Agapornis spp.), in the absence of sunflower seed, selected smaller seeds (canary grass and millet seeds). Cockatiels (N. hollandicus) consumed balanced amounts of seed and extruded feed. When sunflower seed was not provided, all three species selected canary grass and millet seeds over extruded feed. Extruded feed was only consistently consumed when offered as the only food source. This selective feeding resulted in excess intake of fat, protein and gross energy and generally a low calcium and imbalanced calcium and phosphorus ratio in diets in which extruded feed was not consistently consumed. Finally, metabolizable energy intake was above the recommended maintenance energy requirement when experimental diets containing sunflower seeds and exclusively extruded feed were offered. Voluntary intake of mixed diets can predispose captive psittacines to nutrient deficiencies, specially especially in the long term.
{"title":"VOLUNTARY INTAKE OF CAPTIVE PSITTACINES FED MIXED DIETS OF SEEDS AND EXTRUDED FEED","authors":"André Saldanha, Rodrigo Girata Machado, Barbara Decker Fernandes, Júlia Caroline de Oliveira, Gabriela Amorim Carvalho, Tatiane Brandão Moreno, C. Rocha","doi":"10.5380/avs.v28i3.90983","DOIUrl":"https://doi.org/10.5380/avs.v28i3.90983","url":null,"abstract":"Psittacines are popular pet birds throughout the world. In the wild these birds consume a varied diet composed of seeds, nuts, flowers and invertebrates, according to seasonal availability. It is rare for captive birds to have a similar lifestyle and such a diverse diet. In captivity, mixed feeds (seeds and extruded feed) are available on the petfood market. However, these mixed diets allow birds to actively eat seeds resulting in non-balanced diets. The aim of this study was to determine selective voluntary intake of different diets based on different types of seeds and extruded feed in three different small/medium psittacine species commonly maintained as pets. Rose-ringed parakeets (P. krameri) preferentially consumed sunflower seeds, while lovebirds (Agapornis spp.), in the absence of sunflower seed, selected smaller seeds (canary grass and millet seeds). Cockatiels (N. hollandicus) consumed balanced amounts of seed and extruded feed. When sunflower seed was not provided, all three species selected canary grass and millet seeds over extruded feed. Extruded feed was only consistently consumed when offered as the only food source. This selective feeding resulted in excess intake of fat, protein and gross energy and generally a low calcium and imbalanced calcium and phosphorus ratio in diets in which extruded feed was not consistently consumed. Finally, metabolizable energy intake was above the recommended maintenance energy requirement when experimental diets containing sunflower seeds and exclusively extruded feed were offered. Voluntary intake of mixed diets can predispose captive psittacines to nutrient deficiencies, specially especially in the long term.","PeriodicalId":8351,"journal":{"name":"Archives of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139334507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Apolinye Fernanda Da Silva Pinheiro, Marco Antonio de Freitas Mendonça, João Paulo Ferreira Rufino, Francisco Alberto de Lima Chaves, Pedro Gabriel Carneiro de Andrade, Lucas de Almeida Reis, Gabriel Albuquerque da Costa, Rebeca Fontenele Moda
– Ascorbic acid, also known as vitamin C, is essential for the development of eggs and chicks due to its various important roles in biological processes such as collagen synthesis, antioxidant activity, iron absorption, immune system support, neurological development and enzyme cofactor. The injection of nutrients in ovo is a valuable technique used in poultry production due to the possibility of early nutrient delivery to chick embryos and improvement of this development. Front this, this study aimed to evaluate the effects of ascorbic acid use to in ovo feeding for poultry embryos. A total of 350 Rhode Island Red fertile eggs with viable embryos were randomly distributed in seven treatments with 50 replicates (eggs) each. The treatments were control (untreated eggs), eggs subjected to a saline solution (0.50% NaCl) and solutions containing increased levels of ascorbic acid (0.25, 0.50, 0.75, 1.00, and 1.25%). Evaluation of hatchability, embryo mortality, chicks’ weight, and gastrointestinal tract development were performed. The increased addition of ascorbic acid in solutions to in ovo feeding caused a linear decrease (P≤0.05) on hatchability, a linear increase (P≤0.05) on intermediary embryo mortality. It was also observed a linear decrease (P≤0.05) in late embryo mortality. In ovo injection of solutions with 0.50% of ascorbic acid provided heavier (P≤0.05) chicks at birth with better (P≤0.05) gizzard development. However, in ovo injection of ascorbic acid did not affect (P>0.05) the general development of the gastrointestinal tract. In conclusion, in ovo injection of ascorbic acid affected hatching characteristics. Increasing levels of ascorbic acid resulted in a linear decrease on hatchability and a sudden increase in intermediary embryo mortality. In ovo injection of solutions with 0.50% of ascorbic acid provided heavier chicks at birth with better gizzard development. However, it did not affect the development of the gastrointestinal tract of chicks with 1 day-old.
{"title":"The use of ascorbic acid in ovo feeding for poultry embryos and its effect on egg hatchability, embryo mortality, chicks’ weight, and gastrointestinal tract development","authors":"Apolinye Fernanda Da Silva Pinheiro, Marco Antonio de Freitas Mendonça, João Paulo Ferreira Rufino, Francisco Alberto de Lima Chaves, Pedro Gabriel Carneiro de Andrade, Lucas de Almeida Reis, Gabriel Albuquerque da Costa, Rebeca Fontenele Moda","doi":"10.5380/avs.v28i3.85499","DOIUrl":"https://doi.org/10.5380/avs.v28i3.85499","url":null,"abstract":"– Ascorbic acid, also known as vitamin C, is essential for the development of eggs and chicks due to its various important roles in biological processes such as collagen synthesis, antioxidant activity, iron absorption, immune system support, neurological development and enzyme cofactor. The injection of nutrients in ovo is a valuable technique used in poultry production due to the possibility of early nutrient delivery to chick embryos and improvement of this development. Front this, this study aimed to evaluate the effects of ascorbic acid use to in ovo feeding for poultry embryos. A total of 350 Rhode Island Red fertile eggs with viable embryos were randomly distributed in seven treatments with 50 replicates (eggs) each. The treatments were control (untreated eggs), eggs subjected to a saline solution (0.50% NaCl) and solutions containing increased levels of ascorbic acid (0.25, 0.50, 0.75, 1.00, and 1.25%). Evaluation of hatchability, embryo mortality, chicks’ weight, and gastrointestinal tract development were performed. The increased addition of ascorbic acid in solutions to in ovo feeding caused a linear decrease (P≤0.05) on hatchability, a linear increase (P≤0.05) on intermediary embryo mortality. It was also observed a linear decrease (P≤0.05) in late embryo mortality. In ovo injection of solutions with 0.50% of ascorbic acid provided heavier (P≤0.05) chicks at birth with better (P≤0.05) gizzard development. However, in ovo injection of ascorbic acid did not affect (P>0.05) the general development of the gastrointestinal tract. In conclusion, in ovo injection of ascorbic acid affected hatching characteristics. Increasing levels of ascorbic acid resulted in a linear decrease on hatchability and a sudden increase in intermediary embryo mortality. In ovo injection of solutions with 0.50% of ascorbic acid provided heavier chicks at birth with better gizzard development. However, it did not affect the development of the gastrointestinal tract of chicks with 1 day-old.","PeriodicalId":8351,"journal":{"name":"Archives of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139340078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fernanda Moura Fernandes Silva, F. Cruz, Marco Antonio de Freitas Mendonça, João Paulo Ferreira Rufino, Pedro Gabriel Carneiro de Andrade, Lucas Duque Melo, Ramon Duque Melo
This study aimed to evaluate the effects of in ovo feeding using albumin on hatchability, hatching weight, embryo mortality, as well as on organ development of Rhode Island Red chicks. A total of 245 fertile eggs with viable embryos were randomly distributed in seven treatments with 35 replicates (eggs) each. The treatments were untreated eggs and eggs treated with a buffered solution (0.50% saline) or solutions containing increased levels of albumin (0.5, 1.0, 1.5, 2.0, and 2.5%). On d 17 of incubation, eggs were injected with solutions containing albumin dissolved in 200 μL of 0.5% sterile saline. After solutions injection, the pinholes in the eggs were sealed with molten paraffin and moved to a hatching machine. Evaluation of the chicks’ weight, hatchability, and embryo mortality was made immediately post hatch. Five viable chicks of each treatment were randomly selected and slaughtered by cervical dislocation in order to evaluate the heart, gastrointestinal organs and region development. Data collected were subjected to polynomial regression (p≤0.05) after a significant ANOVA result. In ovo feeding using albumin directly affected (p<0.05) the hatching characteristics of injected eggs. When eggs were injected with increased levels of albumin, there was a linear decrease (p<0.05) on hatchability and increase on embryo mortality. The increased supplementation of albumin resulted in a linear growth (p<0.05) of gastrointestinal tract areas responsible to digestion (oropharynx + oesophagus, duodenal loop and cecum), and a linear decrease of heart, liver, pro-ventricle, gizzard and gastrointestinal tract areas responsible to absorption (jejunum + ileum and colon + rectum).
{"title":"Effect of in ovo feeding using albumin to Rhode Island Red poultry embryos","authors":"Fernanda Moura Fernandes Silva, F. Cruz, Marco Antonio de Freitas Mendonça, João Paulo Ferreira Rufino, Pedro Gabriel Carneiro de Andrade, Lucas Duque Melo, Ramon Duque Melo","doi":"10.5380/avs.v28i3.85391","DOIUrl":"https://doi.org/10.5380/avs.v28i3.85391","url":null,"abstract":"This study aimed to evaluate the effects of in ovo feeding using albumin on hatchability, hatching weight, embryo mortality, as well as on organ development of Rhode Island Red chicks. A total of 245 fertile eggs with viable embryos were randomly distributed in seven treatments with 35 replicates (eggs) each. The treatments were untreated eggs and eggs treated with a buffered solution (0.50% saline) or solutions containing increased levels of albumin (0.5, 1.0, 1.5, 2.0, and 2.5%). On d 17 of incubation, eggs were injected with solutions containing albumin dissolved in 200 μL of 0.5% sterile saline. After solutions injection, the pinholes in the eggs were sealed with molten paraffin and moved to a hatching machine. Evaluation of the chicks’ weight, hatchability, and embryo mortality was made immediately post hatch. Five viable chicks of each treatment were randomly selected and slaughtered by cervical dislocation in order to evaluate the heart, gastrointestinal organs and region development. Data collected were subjected to polynomial regression (p≤0.05) after a significant ANOVA result. In ovo feeding using albumin directly affected (p<0.05) the hatching characteristics of injected eggs. When eggs were injected with increased levels of albumin, there was a linear decrease (p<0.05) on hatchability and increase on embryo mortality. The increased supplementation of albumin resulted in a linear growth (p<0.05) of gastrointestinal tract areas responsible to digestion (oropharynx + oesophagus, duodenal loop and cecum), and a linear decrease of heart, liver, pro-ventricle, gizzard and gastrointestinal tract areas responsible to absorption (jejunum + ileum and colon + rectum).","PeriodicalId":8351,"journal":{"name":"Archives of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139341705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fernando Aguilar-Vargas, Fabián Carvajal, M. Guzmán, Bernal León
Classical swine fever (CSF) is a highly contagious viral disease that affects pigs and wild boars. The disease can cause significant economic losses in the pig industry and poses a threat to food security. Therefore, it is crucial to have effective surveillance programs to detect and control the disease. The study mentioned above aimed to compare three commercial ELISA kits for the detection of CSF antibodies. The results showed that all three kits had 100% congruence in undiluted samples, indicating that they are highly reliable for use as a screening test on routine samples. Additionally, the study found good reproducibility between technicians with no significant influence on the variation of results. However, the study also identified a low consistency of positive results (37.7%) for the kit IDvet Screen E2 in diluted samples, with significant variations in results from all three kits. This finding suggests that the other two kits, Herdchek E2 and Priocheck E2, may be better suited for detecting animals with low levels of antibodies, allowing for earlier detection of infected animals and the implementation of relevant control measures. In conclusion, the study demonstrates the importance of using high-quality commercial ELISA kits for the surveillance of CSF. The findings suggest that any of the three kits tested could be used as a reliable screening test on routine samples. However, for the detection of animals with low levels of antibodies, the Herdchek E2 and Priocheck E2 kits may be more effective
{"title":"Comparison of three commercial enzyme-linked immunosorbent assays for the diagnosis of Classical Swine Fever","authors":"Fernando Aguilar-Vargas, Fabián Carvajal, M. Guzmán, Bernal León","doi":"10.5380/avs.v28i2.90894","DOIUrl":"https://doi.org/10.5380/avs.v28i2.90894","url":null,"abstract":"Classical swine fever (CSF) is a highly contagious viral disease that affects pigs and wild boars. The disease can cause significant economic losses in the pig industry and poses a threat to food security. Therefore, it is crucial to have effective surveillance programs to detect and control the disease. The study mentioned above aimed to compare three commercial ELISA kits for the detection of CSF antibodies. The results showed that all three kits had 100% congruence in undiluted samples, indicating that they are highly reliable for use as a screening test on routine samples. Additionally, the study found good reproducibility between technicians with no significant influence on the variation of results. However, the study also identified a low consistency of positive results (37.7%) for the kit IDvet Screen E2 in diluted samples, with significant variations in results from all three kits. This finding suggests that the other two kits, Herdchek E2 and Priocheck E2, may be better suited for detecting animals with low levels of antibodies, allowing for earlier detection of infected animals and the implementation of relevant control measures. In conclusion, the study demonstrates the importance of using high-quality commercial ELISA kits for the surveillance of CSF. The findings suggest that any of the three kits tested could be used as a reliable screening test on routine samples. However, for the detection of animals with low levels of antibodies, the Herdchek E2 and Priocheck E2 kits may be more effective","PeriodicalId":8351,"journal":{"name":"Archives of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45682649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cristiane Ribeiro Da Silva, P. C. Vasconcelos, Celso José Bruno de Oliveira, Danilo Tancler Stipp
{"title":"Resistência Antimicrobiana de Enterobactérias em Aves Migratórias no Litoral Paraibano","authors":"Cristiane Ribeiro Da Silva, P. C. Vasconcelos, Celso José Bruno de Oliveira, Danilo Tancler Stipp","doi":"10.5380/avs.v28i2.89520","DOIUrl":"https://doi.org/10.5380/avs.v28i2.89520","url":null,"abstract":"","PeriodicalId":8351,"journal":{"name":"Archives of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43609468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Julia Dall'Anese, Joaquim Dias Antunes Da Silva Junior, C.L.H. Abrahão, L. L. Dias de Castro, Y. Brandão, Ú. Yoshitani, Vanessa Knopp, M. Molento
Thoroughbred horses have been intensely raised for their athletic potential that is correlated with morphological parameters (i.e., body weight - BW, and withers height - WH). Optimum and consistent body development is aimed, but excessive growth rates may lead to the development of orthopedic diseases. This study aimed to generate growth rate curves and prediction models of Thoroughbred horses by analyzing BW and WH data collected monthly over 16 years of 378 animals (23.6 animals/year). The animals were checked from birth to 18 months (160 colts and 181 fillies) on a farm in the south of Brazil. A prediction performance ARIMA model was developed based on the BW and WH of the foals using a maximum and minimum range of 7320 observations. BW and WH were 54,1 kg and 102,5 cm at birth and 397,8 kg and 150,6 cm at 18 months of age, respectively. No differences were found between sex at any age. Moreover, we have established a nonlinear function for the growth curve and on average, foals were expected to get 7.4 times heavier and 1.5 times taller when animals were fully grown. Males showed greater BW uniformity than females, as females had a lower minimum BW than males. Significant statistical differences (P < 0,05%) were reported for BW and WH of foals between pairs of sires highlighting the kinship (paternal) effect on the animals’ development. Seventy-two pairs of stallions showed statistical relevance for BW and 91 for WH. The ARIMA model produced a linear trend of BW and WH for the forecasted years. In conclusion, we recommend that careful sire selection and adequate health (i.e., parasite control, vaccination), and nutrition strategies must be adopted to achieve superior body growth as estimated by the predicting model (positive scenario). The present protocol shall be used in studs worldwide to monitor horse development. The spreadsheet is available on request to the corresponding authors.
{"title":"The use of body growth and kinship data from 16 generations for predicting Thoroughbred performance","authors":"Julia Dall'Anese, Joaquim Dias Antunes Da Silva Junior, C.L.H. Abrahão, L. L. Dias de Castro, Y. Brandão, Ú. Yoshitani, Vanessa Knopp, M. Molento","doi":"10.5380/avs.v1i1.89547","DOIUrl":"https://doi.org/10.5380/avs.v1i1.89547","url":null,"abstract":"Thoroughbred horses have been intensely raised for their athletic potential that is correlated with morphological parameters (i.e., body weight - BW, and withers height - WH). Optimum and consistent body development is aimed, but excessive growth rates may lead to the development of orthopedic diseases. This study aimed to generate growth rate curves and prediction models of Thoroughbred horses by analyzing BW and WH data collected monthly over 16 years of 378 animals (23.6 animals/year). The animals were checked from birth to 18 months (160 colts and 181 fillies) on a farm in the south of Brazil. A prediction performance ARIMA model was developed based on the BW and WH of the foals using a maximum and minimum range of 7320 observations. BW and WH were 54,1 kg and 102,5 cm at birth and 397,8 kg and 150,6 cm at 18 months of age, respectively. No differences were found between sex at any age. Moreover, we have established a nonlinear function for the growth curve and on average, foals were expected to get 7.4 times heavier and 1.5 times taller when animals were fully grown. Males showed greater BW uniformity than females, as females had a lower minimum BW than males. Significant statistical differences (P < 0,05%) were reported for BW and WH of foals between pairs of sires highlighting the kinship (paternal) effect on the animals’ development. Seventy-two pairs of stallions showed statistical relevance for BW and 91 for WH. The ARIMA model produced a linear trend of BW and WH for the forecasted years. In conclusion, we recommend that careful sire selection and adequate health (i.e., parasite control, vaccination), and nutrition strategies must be adopted to achieve superior body growth as estimated by the predicting model (positive scenario). The present protocol shall be used in studs worldwide to monitor horse development. The spreadsheet is available on request to the corresponding authors.","PeriodicalId":8351,"journal":{"name":"Archives of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49348138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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