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In vitro antiparasitic activity of ethanolic leaves extract of Anethum graveolens 茴香乙醇叶提取物体外抗寄生活性研究
4区 农林科学 Q4 VETERINARY SCIENCES Pub Date : 2023-10-01 DOI: 10.1590/1678-4162-13024
S.N. Maodaa, S. Al-Quraishy, R. Abdel-Gaber, A. Alatawi, S.A. Alawwad, E.M. Al-Shaebi
ABSTRACT Natural products are safe environmentally friendly agents and have no negative impact on the environment, they can be used to combat parasitic diseases. Helminthiasis and coccidiosis are parasitic diseases that harm both health and the economy. This research aimed to see how Anethum graveolens leaves extract (AGLE) worked as an anti-parasitic modulator during oocyst sporulation of an Eimeria papillata infection. FT-IR phytochemical analysis revealed the presence of eight compounds. The time required to induce paralysis and death in worms at the highest concentration (200 mg/mL) was 4.57±0.26 and 5.22±0.10 min, respectively. In an in vitro study, AGLE (300 mg/ml) inhibited sporulation by approximately 100% after 72 and 96 hr. AGLE (200, 100, and 50 mg/ml), amprolium, DettolTM, and phenol induced variable inhibition levels at 96 hr of 5.54%, 1.01%, 37.33%, 81.33%, and 89.33%, respectively. Our findings suggest that AGLE has potent anthelmintic and anticoccidial properties that could be further developed into a novel therapeutic agent.
天然产品是一种安全的环境友好剂,对环境没有负面影响,可用于防治寄生虫病。蠕虫病和球虫病是既危害健康又危害经济的寄生虫病。本研究旨在观察菊花叶提取物(AGLE)在乳头状艾美球虫感染卵囊产孢过程中的抗寄生调节剂作用。FT-IR植物化学分析显示存在8种化合物。最高浓度(200 mg/mL)诱导蠕虫麻痹和死亡所需时间分别为4.57±0.26和5.22±0.10 min。在一项体外研究中,AGLE (300 mg/ml)在72和96小时后抑制孢子形成约100%。AGLE(200、100和50 mg/ml)、氨苯铵、detoltm和苯酚在96小时诱导的可变抑制水平分别为5.54%、1.01%、37.33%、81.33%和89.33%。我们的研究结果表明,AGLE具有有效的驱虫和抗球虫特性,可以进一步发展成为一种新的治疗剂。
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引用次数: 0
Presence of Cryptosporidium spp. in calves from dairy herds in Northern Antioquia, Colombia 哥伦比亚安蒂奥基亚北部奶牛群中隐孢子虫的存在
4区 农林科学 Q4 VETERINARY SCIENCES Pub Date : 2023-10-01 DOI: 10.1590/1678-4162-13043
I.T.L. Aguilar, M.P.E. Cadena, B.C.T. López, H.B. Llano
ABSTRACT Cryptosporidium spp. are important enteropathogen protozoan parasites that infect humans and other animals throughout the world. Cryptosporidiosis in cattle industry leads to considerable economic losses due to diarrhea, dehydration, growth retardation, weight loss, and possibly death, however, data on the occurrence ofCryptosporidiumspp. in cattle in Colombia are limited. Therefore, this study aimed to investigate the occurrence and possible factors associated to the excretion of Cryptosporidium spp. oocyst in pre-weaned calves from dairy farms in Northern Antioquia, Colombia. In addition, Sheather’s sugar floatation (SSF), and Modified Ziehl-Neelsen staining (MZN) methods were compared. A total of 41 fecal samples were collected from calves between 1 and 92 days of age of which 23 were positive (56.1%). Crossbreed calves were nine times less susceptible than purebred dairy cattle to excretion of Cryptosporidium spp. oocysts (OR=0.10). MZN was the best technique for the detection of oocysts in fecal samples, however, the mean number of days to detect cryptosporidial oocysts was lower for the SSF method. Cryptosporidium is widespread among calves under 2 months of age in dairy herds of Northern Antioquia, although further investigations considering a greater number of farms and animals are necessary.
隐孢子虫是一种在世界范围内感染人类和其他动物的重要的肠原动物寄生虫。隐孢子虫病在养牛业中引起腹泻、脱水、生长迟缓、体重减轻,甚至可能导致死亡,导致相当大的经济损失,然而,关于隐孢子虫发生的数据。在哥伦比亚的牛中因此,本研究旨在调查哥伦比亚安蒂奥基亚北部奶牛场断奶前犊牛隐孢子虫卵囊的发生情况及其可能的相关因素。此外,还比较了Sheather糖浮法(SSF)和改良Ziehl-Neelsen染色法(MZN)。共采集1 ~ 92日龄犊牛粪便样本41份,其中23份呈阳性(56.1%)。杂交犊牛对隐孢子虫卵囊排泄的敏感性比纯种奶牛低9倍(OR=0.10)。MZN是检测粪便中隐孢子虫卵囊的最佳方法,但SSF法检测隐孢子虫卵囊的平均时间较短。隐孢子虫广泛存在于安蒂奥基亚北部奶牛场2个月以下的犊牛中,但仍有必要对更多的农场和牲畜进行进一步调查。
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引用次数: 0
Effects of total parenteral nutrition associated with glutamine, enteral fluid therapy, with or without glutamine, and fluid therapy on the lipidogram of horses subjected to starvation after laparotomy 含谷氨酰胺的全肠外营养、含或不含谷氨酰胺的肠内液体治疗和液体治疗对剖腹手术后饥饿马的血脂图的影响
4区 农林科学 Q4 VETERINARY SCIENCES Pub Date : 2023-10-01 DOI: 10.1590/1678-4162-12964
U.P. Melo, M.S. Palhares, C. Ferreira, F.O.P. Leme, V.A. Gheller
ABSTRACT In order to evaluate the effect of total parenteral nutrition associated with glutamine and enteral fluid therapy, associated or not with glutamine, on the lipidogram of horses subjected to starvation (phase 1) and refeeding (phase 2), 16 adult healthy horses were used, mixed breed, aged between four and fourteen years and average weight of 248.40 ± 2.28kg, divided into four experimental groups, with four animals per group: Group ENTGL: enteral fluid therapy with electrolytes associated with glutamine; Group PARGL: total parenteral nutrition associated with glutamine; Group ENTFL: enteral fluid therapy with electrolytes; and Group PARFL: parenteral fluid therapy. This study was divided into two phases: phase 1 and phase 2. Phase 1 consisted of performing exploratory laparotomy and beginning of starvation, in addition to the administration of treatments, according to the group, while phase 2 consisted of re-feeding the animals. Total cholesterol values remained within the normal range for the species throughout the experimental period, but there was a slight increase during phase 1, followed by a decrease in values in phase 2. Hypertriglyceridemia in the ENFL, ENTGL and PARFL groups during phase 1 occurred because of the negative energy balance. The provision of enteral fluid therapy with electrolytes and an energy source, with or without glutamine, or parenteral fluid therapy were not effective in reversing the effects of negative energy balance. Thus, therapeutic protocols that combine enteral or parenteral fluid therapy for prolonged periods and food deprivation need additional nutritional support to avoid the deleterious effects of negative energy balance.
摘要为了研究谷氨酰胺相关全肠外营养和谷氨酰胺相关或非谷氨酰胺相关肠内液体治疗对饥饿(第1阶段)和再喂养(第2阶段)马的血脂的影响,选取16匹4 ~ 14岁、平均体重248.40±2.28kg的健康成年杂交马,分为4个实验组,每组4只:用谷氨酰胺相关电解质进行肠内液治疗;PARGL组:谷氨酰胺相关的全肠外营养;ENTFL组:电解质肠内液治疗;PARFL组:肠外输液治疗。本研究分为两个阶段:第一阶段和第二阶段。根据该小组的说法,第一阶段包括进行剖腹探查和开始饥饿,以及给予治疗,而第二阶段包括重新喂养动物。在整个实验期间,该物种的总胆固醇值保持在正常范围内,但在第1阶段略有上升,随后在第2阶段下降。ENFL、ENTGL和PARFL组在第1期出现高甘油三酯血症是由于负能量平衡。提供含电解质和能量源的肠内液体治疗(含或不含谷氨酰胺)或肠外液体治疗对扭转负能量平衡的影响无效。因此,结合长期肠内或肠外液体治疗和食物剥夺的治疗方案需要额外的营养支持,以避免负能量平衡的有害影响。
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引用次数: 0
Study on spatio-temporal prevalence and hematological attributes of bovine Babesiosis in cattle population of Layyah, Southern Punjab, Pakistan 巴基斯坦旁遮普省南部Layyah地区牛巴贝斯虫病时空流行及血液学特征研究
4区 农林科学 Q4 VETERINARY SCIENCES Pub Date : 2023-10-01 DOI: 10.1590/1678-4162-13002
A. Ahmad, Z. Ali, M.H. Lashari
ABSTRACT The present study was conducted to investigate the spatiotemporal prevalence of bovine babesiosis in cattle population (n=376) of Layyah, Pakistan as affected by location, age, breed, gender, and seasons. Blood samples were collected aseptically and assessed for babesiosis through microscopy and PCR, and through automated analyzer for hematological attributes. Overall results of prevalence through PCR in cattle population showed significantly (P≤0.05) lower prevalence of 19.4% (n=72/376) as compared to 37.2% (n=140/276) through microscopy. None of the studied cattle from Cholistani breed were Babesia-positive. However, significantly (P≤0.05) higher prevalence was noticed for crossbred cattle (46.7%, n= 50/107) followed by that in Friesian (16.1%, n= 10/62), Jersey (7%, n= 5/71) and Sahiwal (6.9%, n= 7/101) cattle breeds. Female cattle (19.5%, n= 55/281) and age group 1 (Up to 2 years) (40%, n= 42/105) had higher prevalence of Babesia as ascertained through PCR in comparison to their counterpart groups. Significantly (P≤0.05) higher prevalence of 35.9% (n=60/167) was shown in summer as compared to that in winter season (5.7%, n= 12/209). All the positive samples produced the 490bp amplicons specific and typical for Babesia bigemina. Hemoglobin concentration, erythrocytic count, hematocrit and mean corpuscular volume were significantly (P≤0.05) lower in babesia-positive cattle as compared to healthy ones.
摘要本研究调查了巴基斯坦Layyah牛种群(n=376)中牛巴贝斯虫病的时空流行情况,以及地点、年龄、品种、性别和季节对牛巴贝斯虫病的影响。无菌采集血样,通过显微镜和PCR检测巴贝斯虫病,并通过自动血液学分析仪检测血液特性。通过PCR在牛群中的总体患病率结果显示,与显微镜下的37.2% (n=140/276)相比,前者的患病率为19.4% (n=72/376),显著(P≤0.05)降低。所研究的牛品种中没有巴贝斯虫阳性。其中,杂交牛(46.7%,n= 50/107)的患病率最高(P≤0.05),其次是弗里西亚(16.1%,n= 10/62)、泽西(7%,n= 5/71)和萨希瓦尔(6.9%,n= 7/101)。通过PCR确定,母牛(19.5%,n= 55/281)和年龄1(2岁以下)(40%,n= 42/105)的巴贝斯虫患病率高于其对应组。夏季患病率为35.9% (n=60/167),高于冬季(5.7%,n= 12/209),差异有统计学意义(P≤0.05)。所有阳性样本均产生双巴贝斯虫特异性和典型的490bp扩增子。与健康牛相比,巴贝虫病阳性牛血红蛋白浓度、红细胞计数、红细胞压积和平均红细胞体积显著(P≤0.05)降低。
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引用次数: 0
Assessing transformation methods for group comparisons under violated assumptions: type I error rate and test power 在违反假设下评估群体比较的转换方法:第一类错误率和测试功率
4区 农林科学 Q4 VETERINARY SCIENCES Pub Date : 2023-10-01 DOI: 10.1590/1678-4162-13026
M. Ergin, Ö. Koşkan
ABSTRACT In this study, some transformation methods that are applied when the assumptions of analysis of variance are not met are evaluated in terms of type I error rate and the test power, under circumstances with different distributions, number of groups, number of observations, variance ratios, and different standard deviation differences. The data set used in the study consisted of random numbers generated from N (0,1), and χ2(3) distributions using the random function of the Numpy library in the Python programming language. The logarithmic, square root and root transformations were evaluated on ANOVA based on simulation combinations. It was observed that the transformation techniques of taking the square root after adding 0.5 and 0.375 to the data were relatively more reliable compared to other transformations in terms of type I error rate. However, in every case, type I error rate determined at the beginning of the experiment increased both before and after the transformation was applied. In particular, interestingly, the third and fourth degree root transformations gave better results of test power in the right skewed distribution. In addition, we compared the transformation techniques in question to determine the normality of the data and the homogeneity of variances by a real data.
本文对不满足方差分析假设条件下应用的几种变换方法,在不同分布、不同组数、不同观测数、不同方差比、不同标准差差的情况下,从ⅰ类错误率和检验能力两个方面进行了评价。研究中使用的数据集由使用Python编程语言Numpy库的随机函数从N(0,1)和χ2(3)分布生成的随机数组成。在模拟组合的基础上,对对数、平方根和根变换进行方差分析。我们观察到,就I类错误率而言,对数据加0.5和0.375后取平方根的变换技术相对于其他变换更为可靠。然而,在每种情况下,在实验开始时确定的I型错误率在应用变换之前和之后都有所增加。特别有趣的是,在右偏态分布中,三次和四次根变换给出了更好的测试功率结果。此外,我们比较了所讨论的转换技术,以确定数据的正态性和方差的均匀性。
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引用次数: 0
Isolated Microsporum Canis from a canine nasal cavity bearer of intranasal foreign body and Transmissible Venereal Tumor - Radiografic imaging and rinoscopy - case report 犬鼻内异物及传染性性病肿瘤携带者分离犬小孢子虫-影像学及鼻镜检查1例报告
4区 农林科学 Q4 VETERINARY SCIENCES Pub Date : 2023-10-01 DOI: 10.1590/1678-4162-12855
D.S. Rolemberg, G.N. Toledo, S.D.S. Reis, P.P. Moreira, P.C. Moraes
ABSTRACT Rhinopathies diagnosis in small animals is challenging, especially regarding their etiology. Imaging exams are very valuable tools for diagnostic procedures. The objective here is to report a rare case of rhinitis by Microsporum canis in a 4-year-old male, SRD dog, sneezing and with chronic purulent nasal secretion two weeks after surgical correction of cleft palate, emphasizing the imaging tests importance for a final and assertive diagnosis. Skull radiographs revealed turbinate destruction and two soft tissue amorphous structures with radiopacity at nasal cavity. The presence of a foreign body in the left passage, soaked in mucopurulent secretion associated with fungal plaques, with firm texture were evidenced by rhinoscopy, and identified as M. canis colonies by microbiological examination. In association, red-brown hyperplastic areas biopsied via rhinoscopy were histologically diagnosed as transmissible venereal tumor. It is concluded that such infection can be reported as opportunistic, secondary to local immunosuppression by post-surgical foreign body and nasal TVT. This is the first case to report such a pathogen in the dog, making its insertion in the differential diagnosis of rhinopathies extremely valuable.
小动物的鼻病诊断是具有挑战性的,特别是关于其病因。影像检查是诊断过程中非常有价值的工具。本文的目的是报告一例罕见的犬小孢子虫引起的鼻炎病例,患者为4岁男性,患有SRD犬,在腭裂手术矫正后两周出现打喷嚏和慢性脓性鼻分泌物,强调影像学检查对最终诊断的重要性。颅骨x线片显示鼻甲破坏,鼻腔内有2个软组织无定形结构。鼻镜检查发现左侧通道内有异物,浸在粘液脓性分泌物中,伴有真菌斑块,质地坚硬,微生物学检查确定为犬支原体菌落。与此同时,经鼻镜活检的红棕色增生区在组织学上被诊断为传染性性病肿瘤。结论:这种感染可报告为机会性感染,继发于术后异物和鼻腔TVT引起的局部免疫抑制。这是第一例报告这样的病原体在狗,使其在鼻病的鉴别诊断插入极有价值。
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引用次数: 0
Meningoencephalomyelitis and pneumonia caused by Cryptococcus spp. in a cat - case report 猫隐球菌引起的脑脊髓炎和肺炎1例报告
4区 农林科学 Q4 VETERINARY SCIENCES Pub Date : 2023-10-01 DOI: 10.1590/1678-4162-12947
A.S. Siviero, B.B. Rivas, R. Redaelli, T.M. Veronezi, M. Slaviero, E.C. Oliveira, L. Sonne, F.V.A. Costa
ABSTRACT Cryptococcosis is an important systemic mycosis with worldwide distribution that affects men and also domestic and wild animals. The infection is acquired by inhalation of the fungus or basidiospores present in the environment and may manifest as localized nasal or ocular injury, pulmonary illness or, less commonly, as meningoencephalomyelitis. Long-term treatment is necessary, and the prognosis is reserved. This paper aims to report a case of invasive cryptococcosis in a domestic cat that was presented with neurological signs. It is intended to emphasize the systemic nature of the infection and its possible routes of infection, reinforcing the importance of investigating this disease as a differential diagnosis of clinical manifestations with neurological involvement in cats.
隐球菌病是一种重要的全身性真菌病,在世界范围内分布,影响人类,也影响家畜和野生动物。这种感染是通过吸入存在于环境中的真菌或担子孢子而获得的,可能表现为局部鼻腔或眼部损伤、肺部疾病,或者较不常见的脑膜脑脊髓炎。需长期治疗,预后未定。本文旨在报告一例侵袭性隐球菌病在一个家猫,提出了神经症状。目的是强调感染的全身性及其可能的感染途径,强调研究这种疾病作为猫神经系统受累临床表现的鉴别诊断的重要性。
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引用次数: 0
Status of paratuberculosis in beef cattle In Rio Grande do Sul, Brazil 巴西南巴西大德州肉牛副结核的现状
4区 农林科学 Q4 VETERINARY SCIENCES Pub Date : 2023-10-01 DOI: 10.1590/1678-4162-12996
D.H. Setim, C. Tesser, F. Benedetti, C. Bondan, M.M. Costa, A.C. da Motta
studies on the
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引用次数: 0
Histological and ultrastructural studies on the effect of Cassia alata methanolic leaf extracts against chemically induced lung adenocarcinoma in rats 决明子甲醇叶提取物抗化学诱导大鼠肺腺癌的组织学和超微结构研究
4区 农林科学 Q4 VETERINARY SCIENCES Pub Date : 2023-10-01 DOI: 10.1590/1678-4162-13047
A.S. Fathalla, M.A. Ibrahim, S.R. Mohamed, M.A. Dkhil, F.A. Thagfan, R. Abdel-Gaber, D. Soliman
ABSTRACT The present work aims to evaluate anticancer performance of Cassia alata methanolic leaf extracts (CMLE) in ethyl carbamate-stimulated lung adenocarcinoma (LAD) in differentiation to the function of Cisplatin (CIPL). Rats were divided into four groups: (1) control (CONT), (2) lung-adenocarcinoma (LAD) injected intra-peritoneally with 1g/kg ethyl carbamate once weekly for a month, (3) LAD+CMLE administered 500 mg/kg CMLE orally for the last two months of the experiment, and (4) LAD+CIPL treated group, injected 2.5 mg/kg Cisplatin intraperitoneally once weekly for the last two months of the experiment. Light and electron microscopic examinations revealed adenocarcinoma development in terminal bronchiole besides some histopathological changes in the LAD group such as atypical, exaggerated collagen fibers, increment of mucinous content, and increasing of PCNA positive immunoreactivity whereas electron microscopy investigation exposed that papillary adenocarcinoma originated from Clara cells in the LAD group. The LAD+CMLE treated group showed no tumor masses and nearly all with normal lung histology. It also recovered the normal ultrastructure of bronchiolar Clara cells. CMLE treatment offers a new alternative cure with less toxicity than Cisplatin for lung cancer therapy. Hence, CMLE would be employed as a novel supply of anti-cancer compounds combating lung cancer.
摘要:本研究旨在评价决明子甲醇叶提取物(CMLE)对氨基甲酸乙酯刺激的肺腺癌(LAD)分化到顺铂(CIPL)的抗癌作用。将大鼠分为四组:(1)对照组(CONT),(2)肺腺癌组(LAD)腹腔注射氨基甲酸乙酯1g/kg,每周1次,连续1个月;(3)LAD+CMLE组,实验后2个月口服CMLE 500 mg/kg; (4) LAD+CIPL组,实验后2个月,每周腹腔注射顺铂2.5 mg/kg。光镜和电镜检查发现LAD组除出现非典型、胶原纤维增多、粘液含量增加、PCNA阳性免疫反应性增加等组织病理学改变外,末梢细支气管腺癌发生,电镜检查发现LAD组乳头状腺癌起源于Clara细胞。LAD+CMLE治疗组未见肿瘤肿块,肺组织基本正常。并恢复了细支气管Clara细胞的正常超微结构。CMLE治疗为肺癌治疗提供了一种新的替代治疗方法,其毒性低于顺铂。因此,CMLE将成为一种新的抗肺癌抗癌化合物。
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引用次数: 0
Impact of adding different concentrations of IGF-I and insulin to the semen extender on bull sperm quality post-cryopreservation 在精液填充剂中添加不同浓度的igf - 1和胰岛素对公牛精子冷冻后质量的影响
4区 农林科学 Q4 VETERINARY SCIENCES Pub Date : 2023-10-01 DOI: 10.1590/1678-4162-12946
S.C.C. Pinto, F.A. Souza, R.P. Arruda, M.B.R. Alves, L. Batissaco, L.N. Garcia-Oliveros, M.S. Passarelli, L.C. Carneiro, E.C.C. Celeghini
ABSTRACT This study aimed to evaluate the addition of different concentrations of IGF-I and insulin to egg yolk-based extender to improve bovine semen cryopreservation. Two experiments were developed to evaluate the effects of the additives in two commercial extenders, Botubov® (Experiment 1) and Triladyl® (Experiment 2), both with the same design. Three ejaculates from four bulls (n = 12) were used. Each ejaculate was divided into seven equal fractions for dilution (60x106 spermatozoa/mL) in the following treatments: CON: extender only; IGF100: IGF-I 100ng/mL; IGF200: IGF-I 200ng/mL; INS150: insulin 150µUI/mL; INS200: insulin 200µUI/mL; ASS1: IGF-I 100ng/mL + insulin 150µUI/mL; ASS2: IGF-I 200ng/mL + insulin 200µUI/mL. Semen was cryopreserved by an automated system. Post-thawed sperm were evaluated regarding motility by CASA (Computer-assisted sperm analysis), and membranes by fluorescent probes (H342, PI, FITC-PSA and JC-1). For Botubov® extender, INS150 was more efficient in preserving total and progressive motility, VCL, BCF, plasma and mitochondrial membranes. A similar response was seen when insulin was added to the Triladyl® extender, INS150 was more efficient in preserving sperm motility, plasma membrane integrity and mitochondrial potential. Thus, the addition of insulin 150µUI/mL, regardless of the composition of the extender, contributes to better preserving bovine sperm from the cryopreservation effects.
摘要本研究旨在评价在蛋黄基填充剂中添加不同浓度的igf - 1和胰岛素对牛精液冷冻保存的改善作用。采用相同的设计,开发了两个实验来评估两种商用扩展剂Botubov®(实验1)和Triladyl®(实验2)中添加剂的效果。使用了4头公牛(n = 12)的3次射精。每次射精被分成7等份稀释(60 × 106精子/mL),在以下处理中:CON:仅添加;IGF100: IGF-I 100ng/mL;IGF200: IGF-I 200ng/mL;INS150:胰岛素150µUI/mL;INS200:胰岛素200µUI/mL;ASS1: IGF-I 100ng/mL +胰岛素150µUI/mL;ASS2: IGF-I 200ng/mL +胰岛素200µUI/mL。精液由自动系统冷冻保存。解冻后的精子用CASA(计算机辅助精子分析)评估活力,用荧光探针(H342、PI、FITC-PSA和JC-1)评估膜。对于Botubov®扩展剂,INS150在保持总运动和进行性运动、VCL、BCF、血浆和线粒体膜方面更有效。当向Triladyl®扩展剂中添加胰岛素时,可以看到类似的反应,INS150在保持精子活力,质膜完整性和线粒体潜能方面更有效。因此,无论填充剂的成分如何,添加150 μ UI/mL的胰岛素都有助于更好地保护牛精子免受低温保存的影响。
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引用次数: 0
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Arquivo Brasileiro De Medicina Veterinaria E Zootecnia
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