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Semen collection using electroejaculation and sperm parameters in pacas (Cuniculus paca, Linnaeus, 1766) 使用电射精和pacas中精子参数采集精液(Cuniculus paca,Linnaeus,1766)
IF 0.4 4区 农林科学 Q4 VETERINARY SCIENCES Pub Date : 2023-07-01 DOI: 10.1590/1678-4162-12799
A.L. Faino, P. Santos, I. O. Farikoski, F. Souza, V. Ribeiro
ABSTRACT Pacas (Cuniculus paca) are highly hunted animals because of the flavor of their meat, and commercial breeding is recommended. However, this species has a relatively low reproductive rate. This study aimed to collect semen from pacas through electroejaculation and obtain the sperm parameters of this species for the first time. Seven male pacas were used, submitted to an anesthetic protocol before stimulation by an electroejaculator appropriate for the species. The stimulus protocol was performed in three series: series I, 10 stimuli with 1 and 2 V; series II, 10 stimuli with 3 and 4 V; and series III, 10 stimuli with 5 V and interval between series of 2 s. The collected material was evaluated for color, volume, motility, vigor, and concentration. The sperm parameters collected showed a mean volume of 0.43±0.33 mL, concentration of 45.5±42.44×106 sperm/mL, motility of 33.33±32.14%, and mean vigor of 2.6±1.15. In this study, the anesthetic protocol did not seem to favor semen collection by electroejaculation in the pacas. The electrical stimulation protocol was able to stimulate all animals in the study; however, there were few samples with sperm cells and a low rate of motility and vigor in most ejaculates.
骆马(学名:Cuniculus paca)因其肉质鲜美而被大量捕杀,因此建议进行商业养殖。然而,该物种的繁殖率相对较低。本研究旨在通过电射精法采集羊驼精液,首次获得羊驼的精子参数。研究人员使用了7只雄性pacas,在使用适合该物种的电射精器进行刺激之前,将它们置于麻醉状态。刺激方案分为三个系列:系列1,10个刺激,1和2 V;系列II, 10个3 V和4 V刺激;第三组为5 V的10个刺激,每组间隔2 s。收集的材料的颜色,体积,运动性,活力和浓度进行评估。精子平均体积为0.43±0.33 mL,精子浓度为45.5±42.44×106 /mL,活力为33.33±32.14%,活力为2.6±1.15。在这项研究中,麻醉方案似乎不赞成通过电射精在pacas收集精液。电刺激方案能够刺激研究中的所有动物;然而,有精子细胞的样本很少,大多数射精的运动性和活力都很低。
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引用次数: 0
Propolis extract as a feed additive of the Nile tilapia juveniles 蜂胶提取物作为尼罗罗非鱼幼鱼饲料添加剂的研究
IF 0.4 4区 农林科学 Q4 VETERINARY SCIENCES Pub Date : 2023-07-01 DOI: 10.1590/1678-4162-12806
E. L. Santos, J. M. Barbosa, F.F. Porto-Neto, J. V. Ludke, T. J. Silva, M. R. Lima, E. C. Soares, Maria C M M Ludke
ABSTRACT This study was conducted to investigate the effects of dietary propolis alcohol extract supplementation on growth performance, digestibility, intestinal morphometry, and physiologic parameters Nile tilápia (Oreochromis niloticus L.) juveniles. For performance experiment and intestinal morphometry, 120 fish averaging 1.41±0.3g were randomly distributed into 24 tanks (70L each) in a closed recirculation water system with constant aeration and biofilter. The animals were distributed into four treatments using a completely randomized design with six replicates per treatment. The treatments consisted of four pellet feeds with different alcohol propolis concentrations extract (AEP) (0.0, 0.5, 1.0 and 1.5gkg-1). The performance experiment period was conducted for 45 days. For the digestibility experiment, an inert marker in the feed and the adapted Guelph feces collecting system were used. The results showed no differences in the performance parameters, crude protein apparent digestibility and dry matter of the feeds, intestinal morphometry, and physiologic parameters. However, there was an increasing linear effect on the energy digestibility with increasing PAE concentrations in the fish feed. The usage of the propolis extract supplementation levels of 1% is recommended for optimum effect on villus morphometry in Nile tilapia.
摘要本研究旨在研究日粮中添加蜂胶醇提取物对尼罗罗非鱼(Oreochromis niloticus L.)幼鱼生长性能、消化率、肠道形态计量学和生理参数的影响。在性能实验和肠道形态测量方面,将120条鱼平均1.41±0.3g随机分配到24个水箱中(每个70L),该水箱位于具有恒定曝气和生物过滤器的封闭再循环水系统中。使用完全随机设计将动物分为四个治疗组,每个治疗组有六个重复。采用四种不同浓度的蜂胶醇提取物(AEP)(0.0、0.5、1.0和1.5gkg-1)颗粒饲料进行处理,试验期为45天。对于消化率实验,使用饲料中的惰性标记物和改良的圭尔夫粪便收集系统。结果表明,饲料的性能参数、粗蛋白表观消化率和干物质、肠道形态计量学和生理参数没有差异。然而,随着鱼类饲料中PAE浓度的增加,对能量消化率的线性影响越来越大。建议使用1%的蜂胶提取物补充水平,以达到对尼罗罗非鱼绒毛形态测定的最佳效果。
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引用次数: 0
Occurrence of hyperostosis in leatherjacket, Oligoplites saurus (Bloch and Schneider, 1801) from Brazilian coastal waters 巴西沿海水域Oligoplites龙皮夹克(Bloch and Schneider, 1801)骨质增生的发生
IF 0.4 4区 农林科学 Q4 VETERINARY SCIENCES Pub Date : 2023-07-01 DOI: 10.1590/1678-4162-12949
Fernando A. P. Tuna, F. Calixto, M. Luz, L. Muniz, E. Mesquita
ABSTRACT Hyperostotic bones occur in several families of marine teleosts, however there are few reports on its development in fish from Brazilian waters. The present study identified the occurrence of hyperostosis in specimens of Leatherjacket fish Oligoplites saurus, an important commercial species, using radiographic images. Biometric measurements of four specimens were performed and the affected bones were detected in different levels and regions of the skeleton: Supraoccipital, Pterygiophores, Cleithrum, Hemal Spines and Neural Spines. Bones with hyperostosis represented up to 7% of the weight of the eviscerated fish. The pattern found was consistent with previous reports in the literature for other species of the genus Oligoplites, this being the first report of hyperostosis for O. saurus.
骨增厚存在于海洋硬骨鱼的几个科中,但在巴西水域的鱼类中很少有报道。本文利用放射线影像对我国重要的商业物种棱皮鱼(Oligoplites saurus)标本进行了骨质增生的鉴定。对四个标本进行了生物测量,并在骨骼的不同水平和区域检测到受影响的骨骼:枕上骨、翼状嵴、锁骨、血管棘和神经棘。有骨质增生的骨头占全内脏鱼重量的7%。发现的模式与文献中对其他Oligoplites属物种的报道一致,这是第一篇关于龙骨肥大的报道。
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引用次数: 1
Cardiorespiratory/sedative effects of a peptide identified in crotalic venom compared to acepromazine and xylazine in horses 在马的心肺/镇静作用比较在蛇毒中鉴定的肽乙酰丙嗪和噻嗪
IF 0.4 4区 农林科学 Q4 VETERINARY SCIENCES Pub Date : 2023-07-01 DOI: 10.1590/1678-4162-12821
C. Abimussi, M. D. Eneas, B. P. Floriano, G. Picolo
ABSTRACT The purpose of this study was to investigate whether intravenous crotalphine produces significant sedation, as well as physiological changes, in healthy standing horses. Six mares, aged 8 years and weighing 415kg underwent three different treatments in a crossover design: TA (acepromazine: 50μg.kg-1), TC (crotalphine: 0.01μg.kg-1) and TX (xylazine: 1000μg.kg-1), intravenously. At various time points over 60 minutes, physiologic variables were recorded: heart rate, respiratory rate, and rectal temperature. The head height from the ground (HHG) was evaluated in centimeters. Data were analyzed using ANOVA followed by Dunnett’s test or Friedman followed by Dunn’s test, under 5% significance. Heart rate decreased significantly at M5 and M10 compared with Mb in TX (28±7, 26±6 and 40±8 beats/minute-1, respectively; p=0.0004). Respiratory rate and rectal temperature did not differ among groups or time points. The HHG significantly decreased in all groups compared with Mb at various time points (p<0.0001). In conclusion, crotalphine did not produce reliable and durable sedation in healthy standing mares and did not influence cardiorespiratory variables in a clinically relevant manner.
本研究的目的是研究静脉注射克罗酞碱是否对健康站立的马产生显著的镇静作用以及生理变化。6匹8岁、体重415kg的母马采用交叉设计,分别静脉注射TA(乙酰丙嗪:50μg.kg-1)、TC (crotalphine: 0.01μg.kg-1)和TX (xylazine: 1000μg.kg-1)。在60分钟内的不同时间点,记录生理变量:心率、呼吸频率和直肠温度。头距地面高度(HHG)以厘米为单位进行评估。数据分析采用方差分析加Dunnett检验或Friedman加Dunn检验,显著性在5%以下。与Mb相比,M5和M10的心率分别显著降低(28±7、26±6和40±8次/分钟-1);p = 0.0004)。呼吸频率和直肠温度在组间和时间点间无差异。与Mb相比,各时间点各组HHG均显著降低(p<0.0001)。综上所示,crotalphine不能对健康站立的母马产生可靠和持久的镇静作用,也不会以临床相关的方式影响心肺变量。
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引用次数: 0
Effect of castration and vasectomy on some oxidative stress parameters and blood hormone levels in rats 去势和输精管切除术对大鼠某些氧化应激参数和血液激素水平的影响
IF 0.4 4区 农林科学 Q4 VETERINARY SCIENCES Pub Date : 2023-07-01 DOI: 10.1590/1678-4162-12982
T. Sancak, V. Koşal, Ç. Okulmuş
ABSTRACT Vasectomy and castration are the most preferred surgical methods to control reproduction in males. While sexual functions are terminated reversibly in vasectomy, they are removed irreversibly in castration. After these processes, changes are observed in hormones and oxidative stress parameters. In this study, we investigated the effects of vasectomy and castration operations on blood follicle stimulating hormone (FSH), luteinizing hormone (LH), testosterone, nitric oxide (NO) and malondialdehyde (MDA) levels in rats. As a result of the analysis, it was determined that FSH, LH, NO, and MDA levels increased (p<0.05) and testosterone levels decreased (p<0.05) in the castration group compared to the sham and vasectomy groups. Considering the data obtained from the present study, when the two operations (vasectomy and castration) are compared in rats, which are preferred for the control of reproduction, it is thought that vasectomy is a healthier method because it is reversible, does not affect hormone levels, and does not increase oxidative stress.
摘要输精管切除术和去势术是控制男性生殖的首选手术方法。性功能在输精管切除术中可逆地终止,而在去势术中则被不可逆转地去除。在这些过程之后,观察到激素和氧化应激参数的变化。在本研究中,我们研究了输精管切除术和去势手术对大鼠血液中卵泡刺激素(FSH)、黄体生成素(LH)、睾酮、一氧化氮(NO)和丙二醛(MDA)水平的影响。分析结果表明,与假手术组和输精管切除术组相比,去势组的FSH、LH、NO和MDA水平升高(p<0.05),睾酮水平降低(p<0.05)。考虑到从本研究中获得的数据,当在大鼠中比较这两种手术(输精管切除术和阉割)时,人们认为输精管切除术是一种更健康的方法,因为它是可逆的,不会影响激素水平,也不会增加氧化应激。
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引用次数: 0
Effects of multimodal environmental modification in crystal-related feline lower urinary tract diseases 多模式环境修饰对晶体相关猫下尿路疾病的影响
IF 0.4 4区 农林科学 Q4 VETERINARY SCIENCES Pub Date : 2023-07-01 DOI: 10.1590/1678-4162-12974
H. Kurum, E. Yalcin
ABSTRACT This study aims to analyze the effects of multimodal environmental modification (MEMO) of cats with recurrence of lower urinary tract signs (LUTS). Treatment of FLUTD included pharmacological treatment, dietary management, and multimodal environmental modification approaches. Twenty client-owned indoor-housed cats with recurrence of lower urinary tract signs had been covered in this observation. Diagnosis of lower urinary tract was made primarily based on the cat's clinical signs, results of laboratory parameters, urinalysis and ultrasonographic examination. Cats were divided into two groups as cefovecin (8 mg/kg sc, single time use) + meloxicam (0.1mg/kg q24h, 3 days) + dry food including l-tryptophan and milk protein hydrolysate to dissolve struvite stones (Group 1) and cefovecin + meloxicam + dry food including l-tryptophan and milk protein hydrolysate to dissolve struvite stones + multimodal environmental modification (Group 2). Clinical and urinary parameters are scored as 0, 1, 2, 3 or 4 points on initial, third, seventh days. Scores of clinical parameters were significantly different between (p<0.05) group 2 and group 1 on the third and seventh days and scores of urinary parameters are significantly different between (p<0.05) group 2 than group 1 on the seventh day. Clients who were contacted after 1 year reported that they did not see any clinical signs of urinary tract diseases.
摘要本研究旨在分析多模式环境修饰(MEMO)对下尿路症状(LUTS)复发猫的影响。FLUTD的治疗包括药物治疗、饮食管理和多模式环境改造方法。本次观察涵盖了20只客户拥有的下尿路症状复发的室内饲养猫。下尿路的诊断主要根据猫的临床症状、实验室参数、尿液分析和超声检查结果。将猫分为两组:头孢维辛(8mg/kg sc,一次性使用)+美洛昔康(0.1mg/kg q24h,3天)+含l-色氨酸和乳蛋白水解物的干粮溶解鸟粪石(第1组)和头孢维辛+美洛西康+含l-色氨酸和奶蛋白水解物溶解鸟粪石干粮+多模式环境修饰(第2组)。临床和泌尿系统参数在第一天、第三天、第七天分别为0分、1分、2分、3分或4分。第3天和第7天,第2组和第1组的临床参数得分有显著差异(p<0.05),第7天第2组与第1组之间的尿参数得分有明显差异(p>0.05)。1年后联系的客户报告说,他们没有看到任何尿路疾病的临床症状。
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引用次数: 0
The effect of feeding frequency on the performance of juvenile cobia (Rachycentron canadum) 饲喂频率对加拿大玉米芯幼鱼生产性能的影响
IF 0.4 4区 农林科学 Q4 VETERINARY SCIENCES Pub Date : 2023-07-01 DOI: 10.1590/1678-4162-12969
J. Motta, A. B. Souza, M. Polese, L. Glória, F. Bosisio, P. P. Mendonça, M. V. Vidal Jr.
bimaculatus (Hayashi et al ., 2004) and Odontesthes humensis (Pouey et al ., 2012), different authors have demonstrated that food frequency can affect survival. Thus, for juvenile cobias (2.0 g), it was observed that the feeding frequency of eight feeds per day, together with an ad libitum feeding rate, was suitable for improving the final weight of these fish, without causing problems like intraspecific competition or decreased survival.
{"title":"The effect of feeding frequency on the performance of juvenile cobia (Rachycentron canadum)","authors":"J. Motta, A. B. Souza, M. Polese, L. Glória, F. Bosisio, P. P. Mendonça, M. V. Vidal Jr.","doi":"10.1590/1678-4162-12969","DOIUrl":"https://doi.org/10.1590/1678-4162-12969","url":null,"abstract":"bimaculatus (Hayashi et al ., 2004) and Odontesthes humensis (Pouey et al ., 2012), different authors have demonstrated that food frequency can affect survival. Thus, for juvenile cobias (2.0 g), it was observed that the feeding frequency of eight feeds per day, together with an ad libitum feeding rate, was suitable for improving the final weight of these fish, without causing problems like intraspecific competition or decreased survival.","PeriodicalId":8393,"journal":{"name":"Arquivo Brasileiro De Medicina Veterinaria E Zootecnia","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44807747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genetic variability of Mycoplasma synoviae detected in commercial layers in southeastern Brazil 巴西东南部商业蛋鸡滑膜支原体的遗传变异性
IF 0.4 4区 农林科学 Q4 VETERINARY SCIENCES Pub Date : 2023-07-01 DOI: 10.1590/1678-4162-12950
C.C. Silva, T. S. Dias, Karolyne S Silva, L. Machado, A. A. Figueira, M. L. Barreto, D.S. Fialho, V. Pereira, E. Nascimento
{"title":"Genetic variability of Mycoplasma synoviae detected in commercial layers in southeastern Brazil","authors":"C.C. Silva, T. S. Dias, Karolyne S Silva, L. Machado, A. A. Figueira, M. L. Barreto, D.S. Fialho, V. Pereira, E. Nascimento","doi":"10.1590/1678-4162-12950","DOIUrl":"https://doi.org/10.1590/1678-4162-12950","url":null,"abstract":"","PeriodicalId":8393,"journal":{"name":"Arquivo Brasileiro De Medicina Veterinaria E Zootecnia","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43956045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of lunging exercise program with Pessoa training aid on cardiac physical conditioning predictors in adult horses 佩索阿训练辅助下的弓步运动计划对成年马心脏体能预测指标的影响
IF 0.4 4区 农林科学 Q4 VETERINARY SCIENCES Pub Date : 2023-07-01 DOI: 10.1590/1678-4162-12972
R. F. Siqueira, M. Teixeira, F.P. Perez, L.S. Gulart
ABSTRACT The aim of this study was to evaluate the effect the Pessoa training aid (PTA) exercise program exerts in some physical conditioning predictors. Eight detrained adult horses were evaluated in 12 sessions of work with PTA (3 sessions per week). All horses used a heart rate monitor and GPS (V800, Polar Electro) and data was used to calculate energy expenditure (EE), net cost of transport (COT), metabolic energy requirement (Pmet), oxygen pulse, oxygen utilization, heart rate and heart rate variability (HRV). The horses were weighted, and the thoracolumbar shape were measured at the level of the 18th (T18), 13th (T13) and 8th (T8) thoracic vertebrae with a flexible ruler before and after the experimental period. Data obtained weekly were submitted to ANOVA and Tukey test (p≤0.05). Data obtained just before and after the experimental period were submitted to paired t test. There was a decrease in left-right asymmetry. In the third week there was an increase in HR, EE, oxygen pulse and oxygen utilization followed by a decrease in the fourth week. The biomechanics related parameters, COT and Pmet decreased week by week. The HRV showed a sympathetic stimulus in the third week followed by a shift to parasympathetic in the fourth week. We conclude that 12 sessions of lunge exercise with PTA contributed to physical condition improvement.
摘要本研究的目的是评估Pessoa训练辅助(PTA)运动计划在一些身体调节预测指标中的作用。8匹去训练的成年马在12次PTA工作中进行评估(每周3次)。所有马使用心率监测仪和GPS (V800, Polar Electro),并使用数据计算能量消耗(EE)、净运输成本(COT)、代谢能需求(Pmet)、氧脉冲、氧利用、心率和心率变异性(HRV)。实验前后对马进行称重,用柔性尺测量第18 (T18)、第13 (T13)、第8 (T8)胸椎水平的胸腰椎形状。每周获得的数据进行方差分析和Tukey检验(p≤0.05)。实验前后获得的数据进行配对t检验。左右不对称性有所减少。第3周HR、EE、氧脉冲和氧利用率增加,第4周下降。生物力学相关参数、COT、Pmet均呈下降趋势。HRV在第三周表现为交感刺激,在第四周转向副交感刺激。我们的结论是,12次的弓步运动与PTA有助于身体状况的改善。
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引用次数: 0
Collecting information on estrus in cattle using the internet of things 利用物联网收集牛发情信息
IF 0.4 4区 农林科学 Q4 VETERINARY SCIENCES Pub Date : 2023-07-01 DOI: 10.1590/1678-4162-12940
K. A. Gündüz, F. Basçiftçi
ABSTRACT Monitoring the movements of ruminant animals is one of the most challenging tasks. In animals that act according to their habits, it is difficult to label such movements and transfer them to farmers. Monitoring and recording the movement and behavior of animals on a farm is an adopted method for successfully determining the duration of the estrus cycle in ruminant animals. The Internet is a technology that offers remarkable solutions for such applications. The aim of this study is to determine the hourly step counts and to find the estrus period in the most accurate way with a circuit design applied to the ankles of animals using an IoT-supported microcontroller. The data is transferred to the web environment wirelessly and monitored via wi-fi communication signals. This wireless wearable and network equipment determines the step count and monitors the animal's abnormal body temperature. An IoT-supported microcontroller provides wireless communication, high-speed data transmission, and low power consumption. Insemination was ensured by testing different animals on the application farm. The data is monitored in real-time, and the system gives an alert. Low cost, high reliability, and being able to be watched over the internet are the advantages of the system. This study helped develop new techniques and provided a low-cost proposition for testing wearable technologies on animals.
摘要监测反刍动物的运动是最具挑战性的任务之一。在根据习惯行事的动物身上,很难给这种运动贴上标签,并将其转移给农民。监测和记录农场动物的运动和行为是成功确定反刍动物发情周期持续时间的一种常用方法。互联网是一种为此类应用提供卓越解决方案的技术。本研究的目的是通过使用物联网支持的微控制器将电路设计应用于动物的脚踝,以最准确的方式确定每小时的步数,并找到发情期。数据被无线传输到网络环境,并通过wi-fi通信信号进行监控。这种无线可穿戴和网络设备可以确定步数,并监测动物的异常体温。物联网支持的微控制器提供无线通信、高速数据传输和低功耗。通过在应用农场测试不同的动物来确保受精。数据被实时监控,系统会发出警报。该系统的优点是成本低、可靠性高,并且能够在互联网上观看。这项研究有助于开发新技术,并为在动物身上测试可穿戴技术提供了一个低成本的建议。
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引用次数: 0
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Arquivo Brasileiro De Medicina Veterinaria E Zootecnia
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