Pub Date : 2023-07-18DOI: 10.37489/0235-2990-2023-68-3-4-39-45
T. Bogush, P. D. Potselueva, A. Scherbakov, E. Bogush, A. B. Ravcheeva, N. Vikhlyantseva, V. Kosorukov
Background. Estrogen receptors beta (ERβ) are an important biological regulator and target of antiestrogens, however, unlike estrogen receptors alpha (ERɑ), their significance in the prognosis and treatment of breast cancer remains unclear. Purpose. Evaluation of the ERβ prognostic value in the comparative assessment of frequency and level of the marker expression in groups with good and poor prognosis by Ki-67 proliferative index score in breast cancer. Methods. ERβ expression level (% of cells expressing the marker) in 68 breast tissue samples was quantified by immunofluorescence and flow cytometry. Primary antibodies to ERβ (clone 14C8, ab288) and secondary antibodies conjugated with DyLight650 (ab98729) were used. In the same samples, the Ki-67 expression level was assessed by the immunohistochemical method. Results. The ERβ and Ki-67 were detected in 100% breast tissue samples with high heterogeneity of the markers’ expression in different patients. Statistical analysis of good and poor prognosis in accordance with the Ki-67 proliferative index score (Ki-67≤20% and Ki-67>20%) showed the prognostic value of the ERβ expression level of 50%. There was no association between the Ki-67 and ERβ expression levels in the same tumor sample (Spearman's rank correlation coefficient R=–0,16; P>0,05). At the same time, high expression of ERβ≥50% was 2,3 times more frequently detected in the good vs poor prognostic group by Ki-67 — 41% vs 18%, P=0,02. Conclusion. The ERβ expression level ≥50% in the tumor can be considered as a factor of good prognosis of breast cancer.
{"title":"Association of the Estrogen Receptors Beta Expression with the Ki-67 Proliferative Index in Breast Cancer","authors":"T. Bogush, P. D. Potselueva, A. Scherbakov, E. Bogush, A. B. Ravcheeva, N. Vikhlyantseva, V. Kosorukov","doi":"10.37489/0235-2990-2023-68-3-4-39-45","DOIUrl":"https://doi.org/10.37489/0235-2990-2023-68-3-4-39-45","url":null,"abstract":"Background. Estrogen receptors beta (ERβ) are an important biological regulator and target of antiestrogens, however, unlike estrogen receptors alpha (ERɑ), their significance in the prognosis and treatment of breast cancer remains unclear. Purpose. Evaluation of the ERβ prognostic value in the comparative assessment of frequency and level of the marker expression in groups with good and poor prognosis by Ki-67 proliferative index score in breast cancer. Methods. ERβ expression level (% of cells expressing the marker) in 68 breast tissue samples was quantified by immunofluorescence and flow cytometry. Primary antibodies to ERβ (clone 14C8, ab288) and secondary antibodies conjugated with DyLight650 (ab98729) were used. In the same samples, the Ki-67 expression level was assessed by the immunohistochemical method. Results. The ERβ and Ki-67 were detected in 100% breast tissue samples with high heterogeneity of the markers’ expression in different patients. Statistical analysis of good and poor prognosis in accordance with the Ki-67 proliferative index score (Ki-67≤20% and Ki-67>20%) showed the prognostic value of the ERβ expression level of 50%. There was no association between the Ki-67 and ERβ expression levels in the same tumor sample (Spearman's rank correlation coefficient R=–0,16; P>0,05). At the same time, high expression of ERβ≥50% was 2,3 times more frequently detected in the good vs poor prognostic group by Ki-67 — 41% vs 18%, P=0,02. Conclusion. The ERβ expression level ≥50% in the tumor can be considered as a factor of good prognosis of breast cancer.","PeriodicalId":8471,"journal":{"name":"Antibiotics and Chemotherapy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79268923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-18DOI: 10.37489/0235-2990-2023-68-3-4-66-75
A. Daudova, Ju. Z. Demina, G. N. Genatullina, R. O. Abdrakhmanova, G. R. Baeva, A. Yasenyavskaya, O. V. Rubalsky
Antimicrobial resistance is considered by WHO as one of the most important threats to public health in the twenty-first century. According to forecasts, by 2025, many first-line antimicrobials will lose their effectiveness and the «post-antibiotic era» will begin. Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacter spp. and representatives of the genus Mycobacterium are classified by the American Society of Infectious Diseases as microorganisms that play a predominant role in the development of infections associated with medical care and leading to fatal consequences. The review highlights the mechanisms of antibiotic resistance and many variants of microbial resistance to antibiotics. Knowledge of the molecular mechanisms of the formation of resistance of microorganisms allows us to develop strategic directions for overcoming it. The search for new ways to prevent and overcome the formation of resistance of pathogens to antibiotics is an extremely important task of modern medical science. The effectiveness of hybrid antibiotics associated with chemical compounds with various specific effects is presented. The use of the main active factor of the bacterial virus, endolysin, both in its pure form and as part of homodimers, for example, lysoprotein, which is a complex of endolysin with human immunoglobulins, is considered promising. Phage therapy of the future is a personalized phage therapy that requires the creation of a library or bank of phages.
{"title":"Antibacterial Resistance. The Challenge of Modernity","authors":"A. Daudova, Ju. Z. Demina, G. N. Genatullina, R. O. Abdrakhmanova, G. R. Baeva, A. Yasenyavskaya, O. V. Rubalsky","doi":"10.37489/0235-2990-2023-68-3-4-66-75","DOIUrl":"https://doi.org/10.37489/0235-2990-2023-68-3-4-66-75","url":null,"abstract":"Antimicrobial resistance is considered by WHO as one of the most important threats to public health in the twenty-first century. According to forecasts, by 2025, many first-line antimicrobials will lose their effectiveness and the «post-antibiotic era» will begin. Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacter spp. and representatives of the genus Mycobacterium are classified by the American Society of Infectious Diseases as microorganisms that play a predominant role in the development of infections associated with medical care and leading to fatal consequences. The review highlights the mechanisms of antibiotic resistance and many variants of microbial resistance to antibiotics. Knowledge of the molecular mechanisms of the formation of resistance of microorganisms allows us to develop strategic directions for overcoming it. The search for new ways to prevent and overcome the formation of resistance of pathogens to antibiotics is an extremely important task of modern medical science. The effectiveness of hybrid antibiotics associated with chemical compounds with various specific effects is presented. The use of the main active factor of the bacterial virus, endolysin, both in its pure form and as part of homodimers, for example, lysoprotein, which is a complex of endolysin with human immunoglobulins, is considered promising. Phage therapy of the future is a personalized phage therapy that requires the creation of a library or bank of phages.","PeriodicalId":8471,"journal":{"name":"Antibiotics and Chemotherapy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91044779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-18DOI: 10.37489/0235-2990-2023-68-3-4-35-38
S. Styazhkina, N. Kiryanov, D. Kuklin, Kh S Kutbedinov, O. N. Eykina, D. V. Strashnov
An experimental study of the antiseptic properties of the Anolyte solution was carried out. The developed anolyte solution has an antiseptic effect, especially active against Escherichia coli, which indicates the possibility of its use in the complex therapy of purulent wounds.
{"title":"Evaluation of the Antiseptic Effect of the Anolyte Solution on Laboratory Rats","authors":"S. Styazhkina, N. Kiryanov, D. Kuklin, Kh S Kutbedinov, O. N. Eykina, D. V. Strashnov","doi":"10.37489/0235-2990-2023-68-3-4-35-38","DOIUrl":"https://doi.org/10.37489/0235-2990-2023-68-3-4-35-38","url":null,"abstract":"An experimental study of the antiseptic properties of the Anolyte solution was carried out. The developed anolyte solution has an antiseptic effect, especially active against Escherichia coli, which indicates the possibility of its use in the complex therapy of purulent wounds.","PeriodicalId":8471,"journal":{"name":"Antibiotics and Chemotherapy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90952906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-18DOI: 10.37489/0235-2990-2023-68-3-4-76-83
M. Varganov, A. A. Miklichev, I. V. Svidersky
The review presents a theoretical rationale for the effectiveness of including topically NPWT therapy and meglumine sodium succinate intravenously in the complex therapy of wounds of various etiologies. The biochemical pathways accompanying the formation of normoxia and hypoxia of varying severity, the significance of succinate in their formation, including depending on the form of its intake into the body (exogenous and endogenous pathways), are given. Thus, when endogenous production of succinic acid is decompensated, the tricarboxylic acid cycle is inhibited and anaerobic glycolysis is activated. At this moment and before the onset of irreversible biochemical damage, the restoration of energy exchange processes begins to depend on succinate, including its exogenous intake. Additional, but no less important, antihypoxic effects of exogenous succinate include stimulation of succinate oxidase oxidation of succinic acid with the restoration of its consumption in the mitochondrial respiratory chain and an increase in the activity of the antioxidant function of glutathione, as well as stimulation of protein metabolism. Reamberin (meglumine sodium succinate) significantly accelerates tissue recovery during hypoxia due to its antihypoxic, cytoprotective and other effects due to the activation of HIF-1ɑ biologically active factors.
{"title":"Substantiation of the Combined Use of Negative Pressure Therapy and Meglumine Sodium Succinate in the Treatment of Wounds of Various Etiologies","authors":"M. Varganov, A. A. Miklichev, I. V. Svidersky","doi":"10.37489/0235-2990-2023-68-3-4-76-83","DOIUrl":"https://doi.org/10.37489/0235-2990-2023-68-3-4-76-83","url":null,"abstract":"The review presents a theoretical rationale for the effectiveness of including topically NPWT therapy and meglumine sodium succinate intravenously in the complex therapy of wounds of various etiologies. The biochemical pathways accompanying the formation of normoxia and hypoxia of varying severity, the significance of succinate in their formation, including depending on the form of its intake into the body (exogenous and endogenous pathways), are given. Thus, when endogenous production of succinic acid is decompensated, the tricarboxylic acid cycle is inhibited and anaerobic glycolysis is activated. At this moment and before the onset of irreversible biochemical damage, the restoration of energy exchange processes begins to depend on succinate, including its exogenous intake. Additional, but no less important, antihypoxic effects of exogenous succinate include stimulation of succinate oxidase oxidation of succinic acid with the restoration of its consumption in the mitochondrial respiratory chain and an increase in the activity of the antioxidant function of glutathione, as well as stimulation of protein metabolism. Reamberin (meglumine sodium succinate) significantly accelerates tissue recovery during hypoxia due to its antihypoxic, cytoprotective and other effects due to the activation of HIF-1ɑ biologically active factors.","PeriodicalId":8471,"journal":{"name":"Antibiotics and Chemotherapy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83619258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-30DOI: 10.37489/0235-2990-2023-68-1-2-27-32
A. Chernikov, D. D. Polyansky, A. Dyakov, K. D. Rotenko, D. V. Panfilova
Relevance. Long-term results of treatment of patients with widespread drug-resistant tuberculosis are not presented in the medical literature. Objective. Study the efficacy and long-term results of treatment of patients with widespread drug-resistant tuberculosis using bedaquiline. Material and methods. Two groups of patients with widespread drug-resistant tuberculosis were formed, who received bedaquiline as the base drug of the chemotherapy course: bedaquiline in the main group (49 people) and moxifloxacin in the control group (76 people). The results of treatment at the end of the course of chemotherapy and after three years of follow-up were studied. Results. 87.8±9.2% of patients from the main group completed an effective course of treatment with sputum culture control. In 67.3±13.1%, the cessation of bacillus excretion was achieved at 1–2 months of treatment. Undesirable effects of chemotherapy were noted in 10.2–32.7% of cases, with prolongation of the QT interval only in 8.2±7.7% of patients. After a three-year dispensary follow-up, there was no recurrence of tuberculosis in the main group, chronic obstructive pulmonary disease was less common for the fifirst time, no cases of treatment by a cardiologist and gynecologist were found, the transfer of patients from the second disability group to the third was more often observed. Conclusion. The use of bedaquiline for the treatment of patients with widespread drug-resistant tuberculosis makes it possible to achieve early cessation of bacillus excretion with transfer to outpatient treatment, reduce the frequency of adverse reactions of chemotherapy. The long-term results of dispensary observation indicate a low risk of reactivation of tuberculosis, a significant reduction in the need for dispensary observation by other specialists, including a cardiologist, and improvement in the results of complex rehabilitation.
{"title":"Long-term results of chemotherapy of tuberculosis with a widespread drug-resistant pathogen using bedaquiline","authors":"A. Chernikov, D. D. Polyansky, A. Dyakov, K. D. Rotenko, D. V. Panfilova","doi":"10.37489/0235-2990-2023-68-1-2-27-32","DOIUrl":"https://doi.org/10.37489/0235-2990-2023-68-1-2-27-32","url":null,"abstract":"Relevance. Long-term results of treatment of patients with widespread drug-resistant tuberculosis are not presented in the medical literature. Objective. Study the efficacy and long-term results of treatment of patients with widespread drug-resistant tuberculosis using bedaquiline. Material and methods. Two groups of patients with widespread drug-resistant tuberculosis were formed, who received bedaquiline as the base drug of the chemotherapy course: bedaquiline in the main group (49 people) and moxifloxacin in the control group (76 people). The results of treatment at the end of the course of chemotherapy and after three years of follow-up were studied. Results. 87.8±9.2% of patients from the main group completed an effective course of treatment with sputum culture control. In 67.3±13.1%, the cessation of bacillus excretion was achieved at 1–2 months of treatment. Undesirable effects of chemotherapy were noted in 10.2–32.7% of cases, with prolongation of the QT interval only in 8.2±7.7% of patients. After a three-year dispensary follow-up, there was no recurrence of tuberculosis in the main group, chronic obstructive pulmonary disease was less common for the fifirst time, no cases of treatment by a cardiologist and gynecologist were found, the transfer of patients from the second disability group to the third was more often observed. Conclusion. The use of bedaquiline for the treatment of patients with widespread drug-resistant tuberculosis makes it possible to achieve early cessation of bacillus excretion with transfer to outpatient treatment, reduce the frequency of adverse reactions of chemotherapy. The long-term results of dispensary observation indicate a low risk of reactivation of tuberculosis, a significant reduction in the need for dispensary observation by other specialists, including a cardiologist, and improvement in the results of complex rehabilitation.","PeriodicalId":8471,"journal":{"name":"Antibiotics and Chemotherapy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73617108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-30DOI: 10.37489/0235-2990-2023-68-1-2-33-44
B. Andryukov, N. Besednova, T. Zaporozhets, A. A. Yakovlev, E. Matosova, S. Kryzhanovsky, M. Shchelkanov
The review presents recent materials about the growing influence of micro- and nanoplastics (MP/NP) on the spread of pathogenic viruses in humans and animals. The issue of the interaction of viruses with the plastisphere, the role of the virus envelope in the preservation of these microorganisms on microparticles in the composition of the biofilm has been elucidated. The issues of synergism of the pathogenetic action of MP/NP and viruses, studied on various models, are discussed. The article shows the negative effect of MP/NP on the permeability of the body's natural barriers, which can create favorable conditions for the penetration of viruses and the development of an infectious process. The authors draw attention to the need to expand interdisciplinary research in this area, given the fact that viruses residing on MP/NP can increase their infectivity and, possibly, form new genovariants.
{"title":"The potential role of micro- and nanoplastics in the spread of viruses","authors":"B. Andryukov, N. Besednova, T. Zaporozhets, A. A. Yakovlev, E. Matosova, S. Kryzhanovsky, M. Shchelkanov","doi":"10.37489/0235-2990-2023-68-1-2-33-44","DOIUrl":"https://doi.org/10.37489/0235-2990-2023-68-1-2-33-44","url":null,"abstract":"The review presents recent materials about the growing influence of micro- and nanoplastics (MP/NP) on the spread of pathogenic viruses in humans and animals. The issue of the interaction of viruses with the plastisphere, the role of the virus envelope in the preservation of these microorganisms on microparticles in the composition of the biofilm has been elucidated. The issues of synergism of the pathogenetic action of MP/NP and viruses, studied on various models, are discussed. The article shows the negative effect of MP/NP on the permeability of the body's natural barriers, which can create favorable conditions for the penetration of viruses and the development of an infectious process. The authors draw attention to the need to expand interdisciplinary research in this area, given the fact that viruses residing on MP/NP can increase their infectivity and, possibly, form new genovariants.","PeriodicalId":8471,"journal":{"name":"Antibiotics and Chemotherapy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87806835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-30DOI: 10.37489/0235-2990-2023-68-1-2-45-56
S. Kovalchuk, L. Fedorova, E. Ilina
Dissemination of microbial resistance to biocides, including disinfectants, is one of the acute problem, which poses biological and economic threats to all countries in the world. Understanding the mechanisms of microbial resistance to disinfectants is a necessary scientific basis for searching ways to overcome it. Despite the wide use of disinfectants, developing bacterial resistance to them has been less studied than to antibiotics. This review presents data on the main molecular mechanisms of microbial resistance to disinfectants.
{"title":"Molecular mechanisms of microbial resistance to disinfectants","authors":"S. Kovalchuk, L. Fedorova, E. Ilina","doi":"10.37489/0235-2990-2023-68-1-2-45-56","DOIUrl":"https://doi.org/10.37489/0235-2990-2023-68-1-2-45-56","url":null,"abstract":"Dissemination of microbial resistance to biocides, including disinfectants, is one of the acute problem, which poses biological and economic threats to all countries in the world. Understanding the mechanisms of microbial resistance to disinfectants is a necessary scientific basis for searching ways to overcome it. Despite the wide use of disinfectants, developing bacterial resistance to them has been less studied than to antibiotics. This review presents data on the main molecular mechanisms of microbial resistance to disinfectants.","PeriodicalId":8471,"journal":{"name":"Antibiotics and Chemotherapy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90875303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-30DOI: 10.37489/0235-2990-2023-68-1-2-69-76
B. Belov, G. Gridneva, E. Aronova, N. Muravyeva
The problem of comorbid infections in rheumatology still remains relevant. Against the background of immunosuppressive/immunomodulatory therapy of immuno-inflammatory rheumatic diseases (IIRD), chronic and opportunistic infections (COI) often develop. Some of these infections can be prevented by performing timely screening procedures and preventive measures. A group of experts from the European Alliance of Rheumatology Associations (EULAR) has prepared recommendations on screening procedures and prevention measures for the most common COI (tuberculosis, chronic viral hepatitis B and C, infections caused by the human immunodeficiency virus and Herpes zoster, pneumocystis pneumonia) within the framework of the IIRD. This article presents the main provisions of this document. The need for periodic review of screening and preventive procedures is emphasized as new scientific data are accumulated.
{"title":"On the issue of screening and prevention of chronic and opportunistic infections in rheumatology","authors":"B. Belov, G. Gridneva, E. Aronova, N. Muravyeva","doi":"10.37489/0235-2990-2023-68-1-2-69-76","DOIUrl":"https://doi.org/10.37489/0235-2990-2023-68-1-2-69-76","url":null,"abstract":"The problem of comorbid infections in rheumatology still remains relevant. Against the background of immunosuppressive/immunomodulatory therapy of immuno-inflammatory rheumatic diseases (IIRD), chronic and opportunistic infections (COI) often develop. Some of these infections can be prevented by performing timely screening procedures and preventive measures. A group of experts from the European Alliance of Rheumatology Associations (EULAR) has prepared recommendations on screening procedures and prevention measures for the most common COI (tuberculosis, chronic viral hepatitis B and C, infections caused by the human immunodeficiency virus and Herpes zoster, pneumocystis pneumonia) within the framework of the IIRD. This article presents the main provisions of this document. The need for periodic review of screening and preventive procedures is emphasized as new scientific data are accumulated.","PeriodicalId":8471,"journal":{"name":"Antibiotics and Chemotherapy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91229898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-30DOI: 10.37489/0235-2990-2023-68-1-2-57-68
Y. Orlov, A. Butrov, S. Sviridov, V. Afanasiev, A. Kondratiev, L. Tsentsiper, N. Govorova, A. Kondratiev, G. A. Baytugaeva, E. N. Kakulya
Purpose. Search and presentation of data from experimental and clinical studies reflecting the central role of succinate and succinate dehydrogenase in the regulation of adaptation mechanisms in critical conditions. Material and methods. The search for literature sources was carried out using the electronic resources of the Russian scientific electronic library eLIBRARY.ru, with the use of the following keywords: «Krebs cycle, succinate, succinate dehydrogenase, critical conditions», as well as in the English-language text database of medical and biological publications on medical sciences PUBMED, where similar keywords «Krebs cycle, succinate, succinate dehydrogenase, critical conditions» were used. Results. Taking into account the data presented in the review, it can be stated that the complex of succinate and succinate dehydrogenase is the central link in the Krebs cycle and the main structure of the antihypoxic ensemble of all body cells during the adaptation period under critical conditions. Conclusion. The data of experimental and clinical studies reflecting the central role of succinate and succinate dehydrogenase not only in the mechanisms of adaptation to hypoxia, but also in the regulation of inflammation, thermogenesis, hemostasis disorders, acid-base state, electrolyte metabolism, vascular tone and trophism of intestinal tissues, as well as in immunological reactions of the body, allow us to consider the substrate and enzyme as the central link of the Krebs cycle and the main structure of the antihypoxic ensemble of all body cells. The data presented in the review open up new prospects for the use of succinates in critical conditions.
{"title":"Succinate and succinate dehydrogenase as a «foothold» in the Krebs cycle in critical conditions","authors":"Y. Orlov, A. Butrov, S. Sviridov, V. Afanasiev, A. Kondratiev, L. Tsentsiper, N. Govorova, A. Kondratiev, G. A. Baytugaeva, E. N. Kakulya","doi":"10.37489/0235-2990-2023-68-1-2-57-68","DOIUrl":"https://doi.org/10.37489/0235-2990-2023-68-1-2-57-68","url":null,"abstract":"Purpose. Search and presentation of data from experimental and clinical studies reflecting the central role of succinate and succinate dehydrogenase in the regulation of adaptation mechanisms in critical conditions. Material and methods. The search for literature sources was carried out using the electronic resources of the Russian scientific electronic library eLIBRARY.ru, with the use of the following keywords: «Krebs cycle, succinate, succinate dehydrogenase, critical conditions», as well as in the English-language text database of medical and biological publications on medical sciences PUBMED, where similar keywords «Krebs cycle, succinate, succinate dehydrogenase, critical conditions» were used. Results. Taking into account the data presented in the review, it can be stated that the complex of succinate and succinate dehydrogenase is the central link in the Krebs cycle and the main structure of the antihypoxic ensemble of all body cells during the adaptation period under critical conditions. Conclusion. The data of experimental and clinical studies reflecting the central role of succinate and succinate dehydrogenase not only in the mechanisms of adaptation to hypoxia, but also in the regulation of inflammation, thermogenesis, hemostasis disorders, acid-base state, electrolyte metabolism, vascular tone and trophism of intestinal tissues, as well as in immunological reactions of the body, allow us to consider the substrate and enzyme as the central link of the Krebs cycle and the main structure of the antihypoxic ensemble of all body cells. The data presented in the review open up new prospects for the use of succinates in critical conditions.","PeriodicalId":8471,"journal":{"name":"Antibiotics and Chemotherapy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85496128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-29DOI: 10.37489/0235-2990-2023-68-1-2-11-15
S. Loginova, V. N. Schukina, S. Savenko, R. V. Sakharov, I. V. Surovyatkina, S. Borisevich
The Сhikungunya virus (CHIKV) is a member of the Flavivirus genus, the Flaviviridae family, and is a zoonotic arbovirus infection transmitted by Aedes mosquitoes. Chikungunya fever causes explosive epidemics involving millions of cases. There is an urgent need to develop broad-spectrum antivirals. Actual is the development of a model of screening in vitro effective drugs. To select the optimal screening conditions, a comparative analysis of cell cultures sensitivity to Сhikungunya virus, strain FN198/66 was carried out. The dynamics and level of virus accumulation at different infectious doses were estimated. It is shown that the Сhikungunya virus, strain FN198/66, well propagated in transplantable cell cultures Vero V, Сl008 Vero, BHK-21/13 and TC-17. All studied cell lines can be used to screen for effective non-specific medical defenses against Сhikungunya virus.
{"title":"Development of the screening conditions in vitro effective drugs against the causative agent of Сhikungunya fever","authors":"S. Loginova, V. N. Schukina, S. Savenko, R. V. Sakharov, I. V. Surovyatkina, S. Borisevich","doi":"10.37489/0235-2990-2023-68-1-2-11-15","DOIUrl":"https://doi.org/10.37489/0235-2990-2023-68-1-2-11-15","url":null,"abstract":"The Сhikungunya virus (CHIKV) is a member of the Flavivirus genus, the Flaviviridae family, and is a zoonotic arbovirus infection transmitted by Aedes mosquitoes. Chikungunya fever causes explosive epidemics involving millions of cases. There is an urgent need to develop broad-spectrum antivirals. Actual is the development of a model of screening in vitro effective drugs. To select the optimal screening conditions, a comparative analysis of cell cultures sensitivity to Сhikungunya virus, strain FN198/66 was carried out. The dynamics and level of virus accumulation at different infectious doses were estimated. It is shown that the Сhikungunya virus, strain FN198/66, well propagated in transplantable cell cultures Vero V, Сl008 Vero, BHK-21/13 and TC-17. All studied cell lines can be used to screen for effective non-specific medical defenses against Сhikungunya virus.","PeriodicalId":8471,"journal":{"name":"Antibiotics and Chemotherapy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78424048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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