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Metabolomics approaches for early cancer diagnosis: a review 代谢组学方法用于早期癌症诊断:综述
IF 2.2 Q2 Agricultural and Biological Sciences Pub Date : 2022-04-01 DOI: 10.35495/ajab.2020.12.566
Cancer remains a major burden on global public health with high mortality rates worldwide. Current diagnosis can detect cancer in late stages when therapy options become limited. Early diagnosis is broadly recognized as the key to a better treatment to save lives. The metabolomics approach provides a better understanding of the different types of cancer. They offer promising and potential interventions in biomarkers discovery which eventually will be better suited for individualized medicine. It elucidates endpoint products for other omic processes while significantly improving the understanding of pathogenesis and mechanisms yet to be discovered. Metabolomics offers a less-invasive, cost-effective for predicting, screening, diagnosis, prognosis, and monitoring therapeutic responses of the disease. There are two methods to study the metabolism and metabolites: targeted and untargeted. The workflow of these approaches requires different analytical platforms, such as Nuclear Magnetic Resonance spectroscopy (NMR), Mass Spectrometry (MS), and different bioinformatic tools. This review provides a systematic summary of metabolomics methods in identifying metabolic biomarkers of cancers (colorectal, prostate, breast, bladder, pancreas, lung, and buccal cancers). In addition, the current review will try to shed light on DNA lesions as a potential metabolic biomarker for cancer.
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引用次数: 1
Nutritional and physical requirements for mycelial growth and fruiting body production of six strains of Pleurotus djamor from Luzon Island, Philippines 菲律宾吕宋岛六株金针菇菌丝生长和子实体生产的营养和物理要求
IF 2.2 Q2 Agricultural and Biological Sciences Pub Date : 2022-02-01 DOI: 10.35495/ajab.2021.05.233
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引用次数: 1
Growth performance of major carps during exposure of zinc and bioaccumulation in fish body organs 锌暴露期间主要鲤鱼的生长性能及鱼体器官的生物蓄积
IF 2.2 Q2 Agricultural and Biological Sciences Pub Date : 2022-01-02 DOI: 10.35495/ajab.2021.02.092
I. Malik, Sawera Nayyab, M. R. Mirza, S. Muzammil, Javaria Saleem Cheema, Komal Imran, Kanwal Nisar, S. Hayat, M. Javed
Growth is an index of metals influence on a fish. This investigation aimed to determine growth responses of major carps viz. Catla catla, Labeo rohita and Cirrhina mrigala during exposure to sublethal concentration to zinc. The variations among fish age, species, treatment (zinc) of growth parameters viz. weight increment, fork lengths, and condition factor were statistically significant at P<0.01. The control fish age group 90,120,150 and 180 day showed better feed conversion ratio 0.234, 0.168, 0.132 and 0.29 as compared to that of zinc exposed fish which showed range 0.01 to 0.23. Three fish species showed significantly higher amount of zinc in liver than other organs. Labeo rohita showed significantly higher trend for zinc accumulation in body organs than as compared to Cirrhina mrigala and Catla catla. Among body organs; skin, muscle, scales displayed significantly lesser trend for zinc accumulation. It is concluded that metal intoxication significantly reduced the growth of all three fish species and may also show good indicator of metals present in surrounding environment.
生长是金属对鱼类影响的一个指标。本研究旨在确定主要鲤鱼,即Catla Catla、Labeo rohita和Cirrhina mrigala在暴露于亚致死浓度的锌时的生长反应。生长参数,即增重、叉长和条件因子,在年龄、物种、处理(锌)之间的差异具有统计学意义,P<0.01。对照鱼90120150和180日龄组的饲料转化率分别为0.234、0.168、0.132和0.29,而锌暴露组的饲料转换率为0.01至0.23。三种鱼类肝脏中的锌含量明显高于其他器官。与Cirrhina mrigala和Catla Catla相比,Labeo rohita在身体器官中的锌积累趋势显著更高。在身体器官之间;皮肤、肌肉和鳞片显示出明显较少的锌积累趋势。结论是,金属中毒显著降低了所有三种鱼类的生长,也可能显示出周围环境中存在金属的良好指标。
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引用次数: 0
Economic potentiality of Colocasia esculenta L. under multipurpose tree-based agroforestry systems 多功能树型农林复合系统下山芋的经济潜力
IF 2.2 Q2 Agricultural and Biological Sciences Pub Date : 2022-01-02 DOI: 10.35495/ajab.2021.01.059
Md. Hafiz All Amin, S. Sumi, W. Jutidamrongphan, K. Techato
Taro (Colocasia esculenta L. cv. Latiraj) is a vegetable for many people in tropical and sub-tropical countries in the world. Because of large quantities of vitamins and minerals, it has considerable value in the food market. As the Taro has raised market demand, it needs to increase production as much as possible. Agroforestry would be a possible production approach for Taro as it can be practised in a partially shaded conditions. The research aimed to evaluate the performance of Taro under woody perennials of Melia azedarach, Leucaena leucocephala, Albizia lebbeck and sole crop cultivation to get potential production and high financial return. This experiment was followed a single factor Randomized Complete Block Design (RCBD) with three replications. The treatments were (1) sole cropping of Latiraj, (2) Latiraj-Melia azedarach, (3) Latiraj-Albizia lebbeck and (4) Latiraj-Leucaena leucocephala. The results showed the maximum number of stolon per plant (29.50) found in LatirajAlbizia lebbeck and noted the minimum number of stolon (19.17) was in LatirajLeucaena leucocephala. Also, the highest stolon length (60.85 cm) and girth (3.33 cm) were observed in Latiraj-Albizia lebbeck and Latiraj-Melia azedarach, respectively. Moreover, the highest stolon yield (10.08 ton/ha) was obtained from Latiraj-Albizia lebbeck compared to (9.85 ton/ha) Latiraj sole cropping. The maximum benefit-cost ratio (4.78) was recorded in the Latiraj-Albizia lebbeck, 24% higher than (3.62) Latiraj sole cropping for economic performance. Finally, it was found that the Taro cv. Latiraj cultivation under Albizia lebbeck as an agroforestry practice would be a sustainable strategy to maximize the production, touching financial benefit and ensuring the proper utilization of vacant woodlots space.
芋头(芋芋)拉蒂拉吉(Latiraj)是世界上热带和亚热带国家许多人的蔬菜。由于含有大量的维生素和矿物质,在食品市场上具有相当大的价值。由于芋头提高了市场需求,因此需要尽可能地增加产量。农林业将是一种可能的芋头生产方法,因为它可以在部分遮荫的条件下进行。本研究旨在评价芋在木本多年生苦楝、银合欢、合欢和单作栽培条件下的表现,以获得潜在的产量和较高的经济回报。本试验采用单因素随机完全区组设计(RCBD), 3个重复。处理分别为:(1)单独种植苦楝、(2)苦楝、(3)苦楝、(4)苦楝。结果表明,单株匍匐茎数最多的品种是白荆(LatirajAlbizia lebbeck)(29.50),最少的品种是白荆(LatirajLeucaena leucocephala)(19.17)。匍匐茎长(60.85 cm)和周长(3.33 cm)最高的分别是白合欢和苦楝。与单作的9.85 t / hm2相比,单作的匍匐茎产量最高,为10.08 t / hm2。在经济效益方面,Latiraj- albizia lebbeck的效益成本比最高(4.78),比Latiraj单独种植的效益成本比(3.62)高24%。最后,研究结果表明,芋头菌种具有良好的生长特性。作为一种农林业实践,紫花苜蓿下的Latiraj种植将是一种可持续的战略,可以最大限度地提高产量,接触经济效益并确保适当利用空闲的林地空间。
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引用次数: 0
Assessment of lead toxicity in red tilapia Oreochromis sp. through hematological parameters 血液学参数对红罗非鱼铅毒性的评价
IF 2.2 Q2 Agricultural and Biological Sciences Pub Date : 2022-01-02 DOI: 10.35495/ajab.2021.01.016
Van-Thanh Vo, T. Duong, Nhat-Anh-Thu Mai, Huyen Nguyen Thi Thuong
Fish is one of the reliable biomonitoring used to assess the levels of heavy metal pollution in the aquatic environment. Analyzing the biological parameters of fish provides a predictable transformation of the underwater ecosystem and the current state of nursery water. Blood directly or indirectly reacts to changes in the environment, objectively reflects the physiological state and allows predicting the direction of adaptive responses in the body. According to the blood indicators and proper generalization of the blood system, we can estimate the physiological and pathological processes inside the body. This study aimed to estimate the effects of lead on hematological parameters in red tilapia. Fish were investigated for the effects of the lead after one week. Fish blood is taken from the tail vein by suction injection. Hematological parameters were determined by standard methods. The number of WBC is counted indirectly through blood smears stained Giemsa. The result of the study shows when the concentration of lead for infection increase from 0mgL -1 (control) to 0.5mgL -1 , 1.0mgL -1 and 1.5mgL -1 , there is a decrease in the hematocrit, hemoglobin, RBC and increases of the mean corpuscular volume, mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration, WBC. RBC and WBC have observed the shape. Blood cells of red tilapia Oriochromis sp. living in a lead polluted environment can be affected and result in many different values of hematological parameters. This is an adaptive reaction of the body in response to harmful factors at the cellular level.
鱼类是用于评估水生环境中重金属污染水平的可靠生物监测之一。通过分析鱼类的生物参数,可以预测水下生态系统和苗圃水的现状。血液直接或间接地对环境的变化做出反应,客观地反映生理状态,并可以预测身体适应性反应的方向。根据血液指标和血液系统的适当概括,我们可以估计身体内部的生理和病理过程。本研究旨在评估铅对红罗非鱼血液学参数的影响。一周后,对鱼类进行了铅的影响调查。鱼血是通过抽吸注射从尾静脉抽取的。通过标准方法测定血液学参数。通过Giemsa染色的血涂片间接计数WBC的数量。研究结果表明,当感染铅浓度从0 mg L-1(对照)增加到0.5 mg L-1、1.0 mg L-1和1.5 mg L-1时,红细胞压积、血红蛋白、红细胞减少,平均红细胞体积、平均红细胞血红蛋白和平均红细胞浓度、白细胞增加。RBC和WBC已观察到形状。生活在铅污染环境中的红罗非鱼的血细胞会受到影响,并导致许多不同的血液学参数值。这是身体在细胞水平上对有害因素的适应性反应。
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引用次数: 1
Growth and biochemical composition of Spirulina platensis dry biomass in diluted monosodium glutamate waste waters 稀释味精废水中钝顶螺旋藻干生物量的生长及生化组成
IF 2.2 Q2 Agricultural and Biological Sciences Pub Date : 2022-01-02 DOI: 10.35495/ajab.2021.05.220
Wardah Wardah, Finalah Nurhayati, Mery Maria Magdalena, N. Fazilah, T. Sopandi
The use of low-cost culture media is important for the development of large-scale Spirulina platensis production. This study aims to investigate the effects of using diluted monosodium glutamate wastewater (MSGW) as a culture medium on the growth and biochemical composition of S. platensis dry biomass. Nitrogen and phosphorus uptake by S. platensis from MSGW media was also investigated in this study. Four concentrations of MSGW have been used in this study, namely 1.5, 2.5, 3.5 and 4.5 mL/L and Walne medium was used as a control. All media were adjusted at pH 9 and S. platensis culture was carried out for 14 day at 36.0C, 77% relative humidity, 5000 lux light intensity and aeration rate at 0.7 L/min. The results of this study have indicated that the growth, protein, lipids, carbohydrates and chlorophyll of S. platensis biomass at 2.5 mL/L MSGW concentrations were not significantly different from Walne medium, but were significantly different from 1.5, 3.5 and 4.5 mL/L MSGW concentrations. The present study have also shown those nitrogen and phosphorus uptakes by S. platensis from 2.5 mL/L MSGW concentration was not significantly different from Walne medium, but were significantly different from 1.5, 3.5 and 4.5 mL/L MSGW concentrations. The present study concluded that S. platensis can be cultivated in MSGW as a medium with an optimum concentration of 2.5 mL/L. This finding will serve as a basic reference for future studies to utilize MSGW for microalgae culture media.
低成本培养基的使用对发展螺旋藻的规模化生产具有重要意义。本研究旨在研究以稀味精废水(MSGW)为培养基对白颡鱼(S. platensis)干生物量生长和生化组成的影响。本研究还研究了白刺对MSGW培养基中氮、磷的吸收。本研究使用了4种浓度的MSGW,分别为1.5、2.5、3.5和4.5 mL/L,并以Walne培养基作为对照。所有培养基调整pH为9,在36.0℃、77%相对湿度、5000 lux光照强度和0.7 L/min曝气速率下培养14 d。本研究结果表明,在2.5 mL/L MSGW浓度下,柽桐生物量的生长、蛋白质、脂质、碳水化合物和叶绿素与Walne培养基差异不显著,但与1.5、3.5和4.5 mL/L MSGW培养基差异显著。本研究还发现,在2.5 mL/L MSGW浓度条件下,平棘藓对氮和磷的吸收量与Walne培养基差异不显著,但与1.5、3.5和4.5 mL/L MSGW浓度条件下差异显著。本研究结果表明,以2.5 mL/L为最佳培养基,在MSGW培养基中可以培养白刺。这一发现将为今后利用MSGW作为微藻培养基的研究提供基础参考。
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引用次数: 1
Chemical composition and anti-diabetic activities in two novel accessions of Cinnamon (Cinnamomum zeylanicum) leaves grown in Sri Lanka 斯里兰卡两种新材料肉桂叶的化学成分和抗糖尿病活性
IF 2.2 Q2 Agricultural and Biological Sciences Pub Date : 2022-01-02 DOI: 10.35495/ajab.2021.03.138
Nimarsha Sonali Kamaradiwela Arachchige, B. Jayawardena
Diabetes Mellitus is a major health concern in many countries. Although there are many synthetic drugs to manage diabetes, most of them are associated with many side effects. Hence there is attention to the use of natural herbs with fewer side effects as an alternative. In this study the hypoglycemic properties, biological activities and chemical composition of leaves of two new Cinnamomum zeylanicum accessions (Sri Wijaya and Sri Gemunu) were investigated using spectrometric techniques. Ethanolic extracts of the two accessions were tested for alpha-amylase inhibitory activity (IC50 of Sri Gemunu 0.0565 ± 0.0013 mg/mL, IC50 of Sri Wijaya 0.1136 ± 0.0011 mg/mL), alpha-glucosidase inhibitory activity (IC50 of Sri Gemunu 0.0347 ± 0.0002 mg/mL, IC50 of Sri Wijaya 0.0884 ± 0.0002 mg/mL), total phenolic content (20.60 ± 0.01 mg Gallic acid equivalents.gof Sri Gemunu, 6.07 ± 0.01 mg Gallic acid equivalents.g of Sri Wijaya), total flavonoid content (72.02 ± 0.02 mg quercetin equivalents.gof Sri Gemunu, 23.59 ± 0.02 mg quercetin equivalents.g of Sri Wijaya), total proanthocyanidin content (0.60 ± 0.05 g catechin equivalents.gof Sri Gemunu, 0.15 ± 0.05 g catechin equivalents.g of Sri Wijaya) and DPPH radical scavenging activity (IC50-58.18 ± 2.53 μg/mL of Sri Wijaya, IC50-69.60 ± 5.48 μg/mL of Sri Gemunu). GC-MS analysis and HPLC analysis indicated that both accessions were rich in eugenol and cinnamaldehyde. According to the results of this study, the ethanolic extract of Sri Gemunu Cinnamomum zeylanicum accession leaves displayed better anti-diabetic properties than the leaves of Sri Wijaya.
糖尿病是许多国家的主要健康问题。虽然有许多合成药物来控制糖尿病,但其中大多数都有许多副作用。因此,人们开始注意使用副作用较小的天然草药作为替代品。本文采用光谱分析技术研究了两种新肉桂(Sri Wijaya和Sri Gemunu)叶片的降糖特性、生物活性和化学成分。测定了两种植物乙醇提取物的α -淀淀酶抑制活性(格努努IC50为0.0565±0.0013 mg/mL,威贾亚IC50为0.1136±0.0011 mg/mL)、α -葡萄糖苷酶抑制活性(格努努IC50为0.0347±0.0002 mg/mL,威贾亚IC50为0.0884±0.0002 mg/mL)、总酚含量(20.60±0.01 mg没食子酸等量)。gof Sri Gemunu, 6.07±0.01 mg没食子酸当量。总黄酮含量(72.02±0.02 mg槲皮素当量)。gof Sri Gemunu, 23.59±0.02 mg槲皮素当量。总原花青素含量(0.60±0.05 g儿茶素当量)。含0.15±0.05克儿茶素当量。清除DPPH自由基的活性(IC50-58.18±2.53 μg/mL, IC50-69.60±5.48 μg/mL)。GC-MS和HPLC分析表明,这两种材料均含有丰富的丁香酚和肉桂醛。本研究结果表明,香樟叶乙醇提取物的抗糖尿病作用优于香樟叶。
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引用次数: 1
Influence of spatial variation on the physicochemical properties and mineralcontent of stingless bee honey (Heterotrigona itama) in Terengganu,Malaysia 马来西亚登嘉楼地区无刺蜂蜂蜜(Heterotrigona itama)理化性质和矿物质含量的空间变化影响
IF 2.2 Q2 Agricultural and Biological Sciences Pub Date : 2022-01-01 DOI: 10.35495/ajab.2021.04.176
The growing interest in the usage of stingless bee honey as a functional food proceeds from its composition, which has been associated with bio-medicinal properties. However, the composition of honey is dependent on the types and origin of bees’ foods, which are flower nectar and plant honeydew. Thus, this study was done to investigate the influence of spatial variation on the physicochemical and mineral content of stingless bee honey in Terengganu, Malaysia. Honeys produced by Heterotrigona itama were collected from five different locations around Terengganu, Malaysia that are surrounded by different plant diversity. The physicochemical properties, antioxidant activity, total phenolic compounds, total flavonoid compounds, and minerals of stingless bee honey were evaluated. Results obtained show that the pH of honey at all locations differed significantly, ranging from 3.30 to 3.54. The sweetness of honey varied between locations, from 69.30 to 75.76 °Brix. The moisture content of honey ranged from 27.28 to 33.13% and the electrical conductivity from 0.65 to 1.46 mScm -1 , which differed significantly among the five selected locations. The parameters for colour, L* (lightness of the sample), a* (the colour scale from red to green) and b* (the colour scale from yellow to blue) values ranged from 25.02 to 52.55, - 0.12 to 7.42, and 10.36 to 14.88, respectively. The total flavonoid content of honey ranged from 1.41 to 6.64 mg CE/g, while total phenolic content gave a value of 3.94 to 10.54 mg GAE/g. The antioxidant activity of honey gave a value ranging from 71.14 to 85.27%. The location of hives had a significant influence on the amount of minerals in stingless bee honey. It can be concluded that the physicochemical properties and mineral content of honey produced by H. itama were different between locations due to the variation of plant diversity at respective locations. The data obtained provides information on the effect of spatial biodiversity variation on the quality of stingless bee honey in selected locations in Terengganu, Malaysia.
{"title":"Influence of spatial variation on the physicochemical properties and mineral\u0000content of stingless bee honey (Heterotrigona itama) in Terengganu,\u0000Malaysia","authors":"","doi":"10.35495/ajab.2021.04.176","DOIUrl":"https://doi.org/10.35495/ajab.2021.04.176","url":null,"abstract":"The growing interest in the usage of stingless bee honey as a functional food proceeds from its composition, which has been associated with bio-medicinal properties. However, the composition of honey is dependent on the types and origin of bees’ foods, which are flower nectar and plant honeydew. Thus, this study was done to investigate the influence of spatial variation on the physicochemical and mineral content of stingless bee honey in Terengganu, Malaysia. Honeys produced by Heterotrigona itama were collected from five different locations around Terengganu, Malaysia that are surrounded by different plant diversity. The physicochemical properties, antioxidant activity, total phenolic compounds, total flavonoid compounds, and minerals of stingless bee honey were evaluated. Results obtained show that the pH of honey at all locations differed significantly, ranging from 3.30 to 3.54. The sweetness of honey varied between locations, from 69.30 to 75.76 °Brix. The moisture content of honey ranged from 27.28 to 33.13% and the electrical conductivity from 0.65 to 1.46 mScm -1 , which differed significantly among the five selected locations. The parameters for colour, L* (lightness of the sample), a* (the colour scale from red to green) and b* (the colour scale from yellow to blue) values ranged from 25.02 to 52.55, - 0.12 to 7.42, and 10.36 to 14.88, respectively. The total flavonoid content of honey ranged from 1.41 to 6.64 mg CE/g, while total phenolic content gave a value of 3.94 to 10.54 mg GAE/g. The antioxidant activity of honey gave a value ranging from 71.14 to 85.27%. The location of hives had a significant influence on the amount of minerals in stingless bee honey. It can be concluded that the physicochemical properties and mineral content of honey produced by H. itama were different between locations due to the variation of plant diversity at respective locations. The data obtained provides information on the effect of spatial biodiversity variation on the quality of stingless bee honey in selected locations in Terengganu, Malaysia.","PeriodicalId":8506,"journal":{"name":"Asian Journal of Agriculture and Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69848040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of the nutritional value, mycochemicals, and antioxidant activitiesof Hericium erinaceus cultivated using jasmine rice 利用茉莉花水稻栽培猴头菌的营养价值、真菌化学物质和抗氧化活性评价
IF 2.2 Q2 Agricultural and Biological Sciences Pub Date : 2022-01-01 DOI: 10.35495/ajab.2021.08.309
Hericium erinaceus is a medicinal mushroom that has various health benefits. The cultivation of mushrooms with solid substrates was previously reported to improve their chemical compositions and bioactivities. The effect of the solid-state cultivation of H. erinaceus using jasmine rice media was evaluated with regard to the nutritional value, mycochemicals, and antioxidant activities of the cultivated products. White jasmine rice (R1) and a mixture of white and red jasmine rice (R2) were used as growth substrates for H. erinaceus . The products of their cultivation, such as rice-fermented mycelia (MR1 and MR2) and basidiomata (BR1 and BR2), were assessed to determine their nutritive contents, mycochemicals, and antioxidant activities compared to unfermented rice (R1 and R2) and commercial basidiome (BS). The nutritional values were measured quantitatively, while the mycochemicals were evaluated qualitatively. The antioxidant activity was measured using the level of DPPH scavenging activity. The results showed that H. erinaceus was successfully cultivated on jasmine rice media. The pH of the media was positively correlated with mycelia growth. The R2-fermented mycelium (MR2) product had higher protein levels (11.40 g/100g) compared to unfermented R2 (6.74 g/100g). Basidiome cultivated on R2 media (BR2) exhibited higher protein levels (15.06 g/100g) compared to commercial basidiome (BS) (10.45 g/100 g). The rice-fermented mycelia contained alkaloids, terpenoids, and saponins. The MR2 sample showed the highest level of antioxidant activity (IC 50 =1.26 mg/ml). These findings suggested that cultivation on jasmine rice enhanced the nutritional value and mycochemical compositions of H. erinaceus , with beneficial antioxidant activity.
{"title":"Evaluation of the nutritional value, mycochemicals, and antioxidant activities\u0000of Hericium erinaceus cultivated using jasmine rice","authors":"","doi":"10.35495/ajab.2021.08.309","DOIUrl":"https://doi.org/10.35495/ajab.2021.08.309","url":null,"abstract":"Hericium erinaceus is a medicinal mushroom that has various health benefits. The cultivation of mushrooms with solid substrates was previously reported to improve their chemical compositions and bioactivities. The effect of the solid-state cultivation of H. erinaceus using jasmine rice media was evaluated with regard to the nutritional value, mycochemicals, and antioxidant activities of the cultivated products. White jasmine rice (R1) and a mixture of white and red jasmine rice (R2) were used as growth substrates for H. erinaceus . The products of their cultivation, such as rice-fermented mycelia (MR1 and MR2) and basidiomata (BR1 and BR2), were assessed to determine their nutritive contents, mycochemicals, and antioxidant activities compared to unfermented rice (R1 and R2) and commercial basidiome (BS). The nutritional values were measured quantitatively, while the mycochemicals were evaluated qualitatively. The antioxidant activity was measured using the level of DPPH scavenging activity. The results showed that H. erinaceus was successfully cultivated on jasmine rice media. The pH of the media was positively correlated with mycelia growth. The R2-fermented mycelium (MR2) product had higher protein levels (11.40 g/100g) compared to unfermented R2 (6.74 g/100g). Basidiome cultivated on R2 media (BR2) exhibited higher protein levels (15.06 g/100g) compared to commercial basidiome (BS) (10.45 g/100 g). The rice-fermented mycelia contained alkaloids, terpenoids, and saponins. The MR2 sample showed the highest level of antioxidant activity (IC 50 =1.26 mg/ml). These findings suggested that cultivation on jasmine rice enhanced the nutritional value and mycochemical compositions of H. erinaceus , with beneficial antioxidant activity.","PeriodicalId":8506,"journal":{"name":"Asian Journal of Agriculture and Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69849350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Synergistic role of pipronyl butoxide in improving the Spodoptera littoralis nucleopolyhedrosis virus effectiveness against Spodoptera littoralis and studying its histopathological biosafety 丁酰哌丙酯在提高滨夜蛾核型多角体病毒抗滨夜蛾有效性及组织病理学生物安全性研究中的协同作用
IF 2.2 Q2 Agricultural and Biological Sciences Pub Date : 2022-01-01 DOI: 10.35495/ajab.2021.05.227
Yasmein A. El Sayed, Rania A. Ahmed, S. Desoky
Spodoptera littoralis nucleopolyhedrosis virus (SlNPV) has been considered as a vital and safe alternative to chemical insecticides. It is effectively used in biological control of Spodoptera littoralis which is considered the most destructive and detrimental economic pest in Egypt. However, its slow speed of action limits its application among other bio-control agents. So, great attention was directed to solve this problem by using insecticide synergists. In this study the toxicity of both SlNPV and the insecticide synergist pipronyl butoxide (PBO) was investigated against 2 nd and 4 th instar S. littoralis larvae. Different concentrations of the tested viral isolate used (10 6 , 10 7 , 10 8 , 10 9 and 10 10 POB/ml) showed high mortality percentage ranging from 60 to 93% and 56 to 83% for the two tested instar larvae respectively 10 days post treatment. The three PBO concentrations used (0.1, 0.2 and 0.3%) revealed weak toxic effect as they produced 13, 20 and 26% mortality rate for the three tested concentrations respectively 4 days post treatment. To examine the biosafety of the three PBO concentrations (0.1, 0.2 and 0.3%), alone or in combination with SlNPV; in vivo animal studies, and in vitro cell line studies were applied. In vivo evaluations included histomorphometric and immunohistochemical alterations in Caspase-3, Proliferating cell nuclear antigen (PCNA), tumor necrosis factor – alpha (TNF-α) in hepatic and renal tissues, plus serum assessments of malondialdehyde (MDA) and total antioxidant capacity (TAC). The sulforhodamine B (SRB) cytotoxicity assay has been applied on OEC (Oral epithelial cell) and Vero (Green monkey kidney) cell lines. Results revealed that 0.1% PBO concentration was safe and didn't induce any pathogenicity, as a dose concentration – dependent decreased pathological changes by comparing 0.3%, 0.2% PBO treated rats . Furthermore, SlNPV was safe to rat tissues and cells and PBO didn't alter the safe nature of SlNPV on mammalian cells. According to the results of histopthological bio-safety, 0.1% of PBO was applied to 4 th instar larvae for estimation its effect on larval enzyme activity and the results proved significant differences in the metabolic tested enzymes between the control and treated larvae with inhibition in both of Actylcholin esterase (AchE), Glutathione S-transferase (GST), Alpha esterases (α-esterases), Beta esterases (Besterases), and Glutamic oxaloacetic transaminase (GOT) and stimulation in Lactate dehydrogenase (LDH), Glutamic pyruvic transaminase (GPT) and Multi-function oxidases (MFO). Moreover, 0.1% of PBO has been mixed with viral LC50 for 2 nd and 4 th instar larvae and the results showed highly synergistic effect of PBO as 100 and 96 % mortality rate respectively fourth day
沿海夜蛾核型多角体病毒(SlNPV)被认为是一种重要而安全的化学杀虫剂替代品。它被有效地用于生物防治被认为是埃及最具破坏性和有害的经济害虫的沿海夜蛾。但其作用速度慢,限制了其在其他生物防治剂中的应用。因此,人们非常重视使用杀虫剂增效剂来解决这一问题。本试验研究了SlNPV和杀虫剂增效剂吡虫腈(PBO)对2龄和4龄海蛾幼虫的毒力。不同浓度的病毒分离物(10 6、10 7、10 8、10 9和10 10 POB/ml)处理10 d后,两种被试幼虫的死亡率分别为60 ~ 93%和56 ~ 83%。使用的三种PBO浓度(0.1、0.2和0.3%)显示出微弱的毒性作用,在治疗后4天,三种测试浓度的死亡率分别为13%、20%和26%。考察三种PBO浓度(0.1、0.2和0.3%)单独或与SlNPV联合使用的生物安全性;采用了体内动物实验和体外细胞系实验。体内评估包括肝和肾组织中Caspase-3、增殖细胞核抗原(PCNA)、肿瘤坏死因子-α (TNF-α)的组织形态学和免疫组化改变,以及血清丙二醛(MDA)和总抗氧化能力(TAC)的评估。应用硫代丹胺B (SRB)细胞毒性试验对OEC (Oral epithelial cell)和Vero (Green monkey kidney)细胞系进行了研究。结果表明,与0.3%、0.2% PBO处理的大鼠相比,0.1% PBO浓度安全且无致病性,其剂量浓度依赖性降低了大鼠的病理变化。此外,SlNPV对大鼠组织和细胞是安全的,PBO没有改变SlNPV对哺乳动物细胞的安全性。根据组织生物安全性结果,将0.1%的PBO应用于4龄幼虫体内,测定其对幼虫酶活性的影响,结果表明,对照与处理后的幼虫代谢检测酶差异显著,对乙酰胆碱酯酶(AchE)、谷胱甘肽s -转移酶(GST)、α酯酶(α-酯酶)、β酯酶(Besterases)、谷草转氨酶(GOT)和乳酸脱氢酶(LDH)、谷丙转氨酶(GPT)和多功能氧化酶(MFO)的刺激。此外,0.1%的PBO与病毒LC50混合用于2龄和4龄幼虫,结果显示PBO具有很强的协同作用,第4天死亡率分别为100%和96%
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引用次数: 0
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Asian Journal of Agriculture and Biology
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