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Silicon improves strawberry plants nutrient uptake and epicuticular wax formation in a rhizosphere cooling system 在根际冷却系统中,硅提高了草莓植株的营养吸收和表皮蜡的形成
IF 2.2 Q2 Agricultural and Biological Sciences Pub Date : 2023-01-01 DOI: 10.35495/ajab.2022.060
Bioavailable silicon (silicic acid) is considered to be beneficial for plant growth and development. This study therefore aimed to determine the effects of silicic acid [Si(OH) 4 ] and potassium bicarbonate (KHCO 3 ) application on strawberry ( Fragaria x ananassa Duch) plants’ nutrient uptake and leaf wax formation. The strawberry plants (cvs Festival and Fortuna) were grown in a rhizosphere cooling system (RCS) at a mean rhizosphere temperature of 18°C ±2. Accumulation of plant nutrients in the plant parts was analysed by using inductively coupled plasma – optical emission spectrometry (ICP-OES), whilst the formation of wax on the leaves was observed with scanning electron microscopy (SEM). The results showed that plants given 0.25% (v/v) Si(OH) 4 via the roots had the highest amount of silicon accumulated in the leaves, roots, and crowns of both cultivars. The greatest amount of Ca and Mg was also found in the leaves of cv. Festival plants treated with 0.25% (v/v) Si(OH) 4 through the roots; whereas in cv. Fortuna, Ca, Mg and P had their highest amount accumulated in the leaves of plants sprayed with 0.25% (v/v) Si(OH) 4 mixed with 0.5% (s/v) KHCO 3 . Root application of the KHCO 3 treatments on the other hand, limited Ca, Mg, Mn and Fe uptake into the plant parts. The leaves of the Si(OH) 4 treated plants were covered with a denser mass of wax when observed under SEM. Thus, Si(OH) 4 application was found to enhance strawberry plants’ uptake of essential nutrients and also improved the formation wax on leaves, that may delay plant diseases invasion.
{"title":"Silicon improves strawberry plants nutrient uptake and epicuticular wax formation in a rhizosphere cooling system","authors":"","doi":"10.35495/ajab.2022.060","DOIUrl":"https://doi.org/10.35495/ajab.2022.060","url":null,"abstract":"Bioavailable silicon (silicic acid) is considered to be beneficial for plant growth and development. This study therefore aimed to determine the effects of silicic acid [Si(OH) 4 ] and potassium bicarbonate (KHCO 3 ) application on strawberry ( Fragaria x ananassa Duch) plants’ nutrient uptake and leaf wax formation. The strawberry plants (cvs Festival and Fortuna) were grown in a rhizosphere cooling system (RCS) at a mean rhizosphere temperature of 18°C ±2. Accumulation of plant nutrients in the plant parts was analysed by using inductively coupled plasma – optical emission spectrometry (ICP-OES), whilst the formation of wax on the leaves was observed with scanning electron microscopy (SEM). The results showed that plants given 0.25% (v/v) Si(OH) 4 via the roots had the highest amount of silicon accumulated in the leaves, roots, and crowns of both cultivars. The greatest amount of Ca and Mg was also found in the leaves of cv. Festival plants treated with 0.25% (v/v) Si(OH) 4 through the roots; whereas in cv. Fortuna, Ca, Mg and P had their highest amount accumulated in the leaves of plants sprayed with 0.25% (v/v) Si(OH) 4 mixed with 0.5% (s/v) KHCO 3 . Root application of the KHCO 3 treatments on the other hand, limited Ca, Mg, Mn and Fe uptake into the plant parts. The leaves of the Si(OH) 4 treated plants were covered with a denser mass of wax when observed under SEM. Thus, Si(OH) 4 application was found to enhance strawberry plants’ uptake of essential nutrients and also improved the formation wax on leaves, that may delay plant diseases invasion.","PeriodicalId":8506,"journal":{"name":"Asian Journal of Agriculture and Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69849823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Metabarcoding of nematode communities associated with Ananas comosus L.(Pineapple) 凤梨线虫群落元条形码研究
IF 2.2 Q2 Agricultural and Biological Sciences Pub Date : 2023-01-01 DOI: 10.35495/ajab.2022.114
L. Zurbano, C. Deocaris, Carmelita P. Mapanao, Arnel O. Rendon, L. V. Alvarez
,
{"title":"Metabarcoding of nematode communities associated with Ananas comosus L.\u0000(Pineapple)","authors":"L. Zurbano, C. Deocaris, Carmelita P. Mapanao, Arnel O. Rendon, L. V. Alvarez","doi":"10.35495/ajab.2022.114","DOIUrl":"https://doi.org/10.35495/ajab.2022.114","url":null,"abstract":",","PeriodicalId":8506,"journal":{"name":"Asian Journal of Agriculture and Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69850323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
High inhibition efficacy of pancreatic cholesterol esterase and porcine pancreatic lipase from natural products 天然产物胰腺胆固醇酯酶和猪胰腺脂肪酶具有较高的抑制效果
Q2 Agricultural and Biological Sciences Pub Date : 2023-01-01 DOI: 10.35495/ajab.2023.041
,
{"title":"High inhibition efficacy of pancreatic cholesterol esterase and porcine pancreatic lipase from natural products","authors":"","doi":"10.35495/ajab.2023.041","DOIUrl":"https://doi.org/10.35495/ajab.2023.041","url":null,"abstract":",","PeriodicalId":8506,"journal":{"name":"Asian Journal of Agriculture and Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135704684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Influence of dietary neutral detergent fiber on feed intake and nutrient digestibility of crossbred Black Angus cattle 饲粮添加中性洗涤纤维对杂交黑安格斯牛采食量和营养物质消化率的影响
IF 2.2 Q2 Agricultural and Biological Sciences Pub Date : 2023-01-01 DOI: 10.35495/ajab.2021.420
The objective of this experiment was to evaluate different neutral detergent fiber (NDF) levels affected on the feed intake and nutrient digestibility of crossbred beef cattle. Four male F 1 (Black Angus x Zebu cross) cattle at 20.5±1.21 (Mean±SD) months of age with average body weight of 299±12.1 kg were used in a 4x4 Latin square design (fourteen days per each period). The treatments were 47% (NDF47), 51% (NDF51), 55% (NDF55), and 59% (NDF59) NDF in diets. The results showed that the dry matter (DM), organic matter (OM), and crude protein (CP) intake were not different (P>0.05) among treatments. However, both NDF and acid detergent fiber (ADF) consumption were lower for NDF 47 than NDF59 treatments (P<0.05), while metabolizable energy was highest for NDF47 compare with NDF59 treatment (P<0.05). The DM digestibility was higher (P<0.05) for the NDF47 (65.3%) compared with NDF59 (57.5%), while NDF47 was similar (P>0.05) to NDF51 (62.4%), and NDF55 (59.4%) treatments. The data for CP, NDF, and ADF digestibility were similar (P>0.05) among treatments. However, there was tended to be lower in NDF59 than NDF47 treatments. The daily weight gain was 866, 821, 786, and 686 g/animal/day for treatments of NDF47, NDF51, NDF55, and NDF59, respectively. While, feed conversion ratio was lower for NDF47 and higher for NDF59 treatments (P>0.05). The conclusion in the present study was 55% NDF in diet could be properly recommended for farmers’ practice in terms of feed utilization.
{"title":"Influence of dietary neutral detergent fiber on feed intake and nutrient digestibility of crossbred Black Angus cattle","authors":"","doi":"10.35495/ajab.2021.420","DOIUrl":"https://doi.org/10.35495/ajab.2021.420","url":null,"abstract":"The objective of this experiment was to evaluate different neutral detergent fiber (NDF) levels affected on the feed intake and nutrient digestibility of crossbred beef cattle. Four male F 1 (Black Angus x Zebu cross) cattle at 20.5±1.21 (Mean±SD) months of age with average body weight of 299±12.1 kg were used in a 4x4 Latin square design (fourteen days per each period). The treatments were 47% (NDF47), 51% (NDF51), 55% (NDF55), and 59% (NDF59) NDF in diets. The results showed that the dry matter (DM), organic matter (OM), and crude protein (CP) intake were not different (P>0.05) among treatments. However, both NDF and acid detergent fiber (ADF) consumption were lower for NDF 47 than NDF59 treatments (P<0.05), while metabolizable energy was highest for NDF47 compare with NDF59 treatment (P<0.05). The DM digestibility was higher (P<0.05) for the NDF47 (65.3%) compared with NDF59 (57.5%), while NDF47 was similar (P>0.05) to NDF51 (62.4%), and NDF55 (59.4%) treatments. The data for CP, NDF, and ADF digestibility were similar (P>0.05) among treatments. However, there was tended to be lower in NDF59 than NDF47 treatments. The daily weight gain was 866, 821, 786, and 686 g/animal/day for treatments of NDF47, NDF51, NDF55, and NDF59, respectively. While, feed conversion ratio was lower for NDF47 and higher for NDF59 treatments (P>0.05). The conclusion in the present study was 55% NDF in diet could be properly recommended for farmers’ practice in terms of feed utilization.","PeriodicalId":8506,"journal":{"name":"Asian Journal of Agriculture and Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69849884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The effects of temperature, pH, and media on mycelium growth of Isariatenuipes (Peck.) Samson (DL0099) from Lang Biang Mountain, Lam VienPlateau, Vietnam 温度、pH和培养基对Isariatenuipes (Peck.)菌丝生长的影响参孙(DL0099),产自越南林越高原朗比山
IF 2.2 Q2 Agricultural and Biological Sciences Pub Date : 2023-01-01 DOI: 10.35495/ajab.2022.179
Isaria tenuipes (Peck.) Samson is an entomopathogenic fungus that has potential in pharmacology and biocontrol. The study aimed to determine the effects of temperature, pH, and media on mycelium growth of Isaria tenuipes (Peck.) Samson (DL0099) from Lang Biang Mountain, Lam Vien Plateau, Vietnam. In this study, we used the single-factor method to culture DL0099 mycelium on both surface liquid and agar media. The results indicated that the optimal temperature for the mycelium growth was 20~25 o C. This mushroom mycelium can tolerate temperatures at 35 o C for 8 days. The initial media pH range of 6~9 was found to be the most favorable to mycelia growth. Sabouraud's agar (SA) and Sabouraud's dextrose agar plus yeast (SDYA) media were the optimal agar media for the mycelium growth rate. Sabouraud broth (S) medium was the most suitable medium for mycelia biomass production in surface liquid culture. Yeast malt agar (YMA), malt agar (MEA), maltose agar (MA), and malt extract yeast agar (MYA) media were suitable for fruit body formation. Our results provided optimal temperature, pH, and media conditions for Isaria tenuipes (DL0099) mycelium growth on surface liquid and agar media. They lay the basis for effective propagation
{"title":"The effects of temperature, pH, and media on mycelium growth of Isaria\u0000tenuipes (Peck.) Samson (DL0099) from Lang Biang Mountain, Lam Vien\u0000Plateau, Vietnam","authors":"","doi":"10.35495/ajab.2022.179","DOIUrl":"https://doi.org/10.35495/ajab.2022.179","url":null,"abstract":"Isaria tenuipes (Peck.) Samson is an entomopathogenic fungus that has potential in pharmacology and biocontrol. The study aimed to determine the effects of temperature, pH, and media on mycelium growth of Isaria tenuipes (Peck.) Samson (DL0099) from Lang Biang Mountain, Lam Vien Plateau, Vietnam. In this study, we used the single-factor method to culture DL0099 mycelium on both surface liquid and agar media. The results indicated that the optimal temperature for the mycelium growth was 20~25 o C. This mushroom mycelium can tolerate temperatures at 35 o C for 8 days. The initial media pH range of 6~9 was found to be the most favorable to mycelia growth. Sabouraud's agar (SA) and Sabouraud's dextrose agar plus yeast (SDYA) media were the optimal agar media for the mycelium growth rate. Sabouraud broth (S) medium was the most suitable medium for mycelia biomass production in surface liquid culture. Yeast malt agar (YMA), malt agar (MEA), maltose agar (MA), and malt extract yeast agar (MYA) media were suitable for fruit body formation. Our results provided optimal temperature, pH, and media conditions for Isaria tenuipes (DL0099) mycelium growth on surface liquid and agar media. They lay the basis for effective propagation","PeriodicalId":8506,"journal":{"name":"Asian Journal of Agriculture and Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69850077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Rose and eucalyptus essential oil as potent anti-liver cancer agents 玫瑰和桉树精油是有效的抗肝癌剂
IF 2.2 Q2 Agricultural and Biological Sciences Pub Date : 2023-01-01 DOI: 10.35495/ajab.2022.141
The present study was designed to investigate the anti-cancer potential of essential oil obtained from Rosa indica (REO) and Eucalyptus citriodora (EEO) against the liver carcinoma cell line (HepG2). Firstly, the cytotoxic activity was assessed using increasing concentrations ranging from 3.12 to 200 µg/ml via MTT assay. EEO showed only 2% cell viability while REO represented 18% at the highest concentration (200 μg/ml). The half-maximal inhibitory concentration (IC 50 ) of EEO and REO was found to be 17.741 µg/ml and 18.55 µg/ml respectively. Additionally, evident morphological changes in HepG2 cells were observed after 24 hours of essential oil treatment compared to control or untreated cells. Furthermore, to strengthen the anti-cancer perspective of essential oils, the anti-metastatic potential was evaluated through the wound healing assay. EEO promisingly inhibited migration (4% wound closure, **p > 0.01) in HepG2 cells after 24 hr treatment. Likewise, REO also exhibited good results (37% wound closure, ***p > 0.001). Conclusively, the present investigation provides preliminary results which suggest that REO and EEO are potent anti-cancer agents against hepatocellular carcinoma.
{"title":"Rose and eucalyptus essential oil as potent anti-liver cancer agents","authors":"","doi":"10.35495/ajab.2022.141","DOIUrl":"https://doi.org/10.35495/ajab.2022.141","url":null,"abstract":"The present study was designed to investigate the anti-cancer potential of essential oil obtained from Rosa indica (REO) and Eucalyptus citriodora (EEO) against the liver carcinoma cell line (HepG2). Firstly, the cytotoxic activity was assessed using increasing concentrations ranging from 3.12 to 200 µg/ml via MTT assay. EEO showed only 2% cell viability while REO represented 18% at the highest concentration (200 μg/ml). The half-maximal inhibitory concentration (IC 50 ) of EEO and REO was found to be 17.741 µg/ml and 18.55 µg/ml respectively. Additionally, evident morphological changes in HepG2 cells were observed after 24 hours of essential oil treatment compared to control or untreated cells. Furthermore, to strengthen the anti-cancer perspective of essential oils, the anti-metastatic potential was evaluated through the wound healing assay. EEO promisingly inhibited migration (4% wound closure, **p > 0.01) in HepG2 cells after 24 hr treatment. Likewise, REO also exhibited good results (37% wound closure, ***p > 0.001). Conclusively, the present investigation provides preliminary results which suggest that REO and EEO are potent anti-cancer agents against hepatocellular carcinoma.","PeriodicalId":8506,"journal":{"name":"Asian Journal of Agriculture and Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69850371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
IAA-producing and phosphate solubilizer of rhizosphere actinobacteria consortium to promote plant growth in soybean (Glycine max L.) 根际放线菌联合体产iaa和增磷剂对大豆生长的促进作用
Q2 Agricultural and Biological Sciences Pub Date : 2023-01-01 DOI: 10.35495/ajab.2021.402
Plant Growth-Promoting Rhizobacteria (PGPR) are microbes that inhabit the rhizosphere and rhizoplanes environment and can enhance plant growth. The application of PGPR in agriculture can reduce the use of chemical fertilizers on soil. The purpose of this study was to determine the ability of the actinobacteria consortium to produce Indole-3-Acetic Acid (IAA), solubilize phosphate, and improve soybean growth in the greenhouse. The results of this study showed that the actinobacteria ( Streptomyces sp. ASR58, Streptomyces sp. ASR67) and Rhizobium sp. which are used in this study are mutually compatible and tolerant to several types of fungicides and bactericides. Meanwhile, the consortium of Streptomyces sp. ASR58 and Streptomyces sp. ASR67 produces the highest concentration of IAA i.e. 25.11 ppm compared to each isolate and other bacterial consortia. Accordance to the quantitative phosphate solubilization assay, Streptomyces sp. ASR67 resulted in the highest dissolved inorganic phosphate i.e. 179.7 + 13.3 mg / L. Inoculation of Streptomyces sp. ASR58 and Streptomyces sp. ASR67 consortium into soybean seeds can significantly increase 54.6% in stem length, 29% root growth, and 20.4% in plant dry weight. This research indicated that Streptomyces sp. ASR58 and Streptomyces sp. ASR67 consortium resulted in the best growth toward soybean plants compared to other bacterial consortia.
{"title":"IAA-producing and phosphate solubilizer of rhizosphere actinobacteria consortium to promote plant growth in soybean (Glycine max L.)","authors":"","doi":"10.35495/ajab.2021.402","DOIUrl":"https://doi.org/10.35495/ajab.2021.402","url":null,"abstract":"Plant Growth-Promoting Rhizobacteria (PGPR) are microbes that inhabit the rhizosphere and rhizoplanes environment and can enhance plant growth. The application of PGPR in agriculture can reduce the use of chemical fertilizers on soil. The purpose of this study was to determine the ability of the actinobacteria consortium to produce Indole-3-Acetic Acid (IAA), solubilize phosphate, and improve soybean growth in the greenhouse. The results of this study showed that the actinobacteria ( Streptomyces sp. ASR58, Streptomyces sp. ASR67) and Rhizobium sp. which are used in this study are mutually compatible and tolerant to several types of fungicides and bactericides. Meanwhile, the consortium of Streptomyces sp. ASR58 and Streptomyces sp. ASR67 produces the highest concentration of IAA i.e. 25.11 ppm compared to each isolate and other bacterial consortia. Accordance to the quantitative phosphate solubilization assay, Streptomyces sp. ASR67 resulted in the highest dissolved inorganic phosphate i.e. 179.7 + 13.3 mg / L. Inoculation of Streptomyces sp. ASR58 and Streptomyces sp. ASR67 consortium into soybean seeds can significantly increase 54.6% in stem length, 29% root growth, and 20.4% in plant dry weight. This research indicated that Streptomyces sp. ASR58 and Streptomyces sp. ASR67 consortium resulted in the best growth toward soybean plants compared to other bacterial consortia.","PeriodicalId":8506,"journal":{"name":"Asian Journal of Agriculture and Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135704886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Biological activity of leaves of three Morinda species detected by stimulation and suppression of gene expression of collagen, elastin, melanin and other related genes 通过刺激和抑制胶原、弹性蛋白、黑色素等相关基因的表达,检测三种桑达植物叶片的生物活性
Q2 Agricultural and Biological Sciences Pub Date : 2023-01-01 DOI: 10.35495/ajab.2023.117
Morinda coreia , M. elliptica and M. tomentosa leaves were investigated. α-EG and pinoresinol were found by GC-MS and GC-FID. MTT and comet assays did not reveal toxicity in normal PBMCs and DHFa cells, but the ethanol and hexane M. coreia and M. tomentosa extracts were toxic to CHL-1 cancer cells. Biological activities were detected by gene expression through qRT-PCR. Ethanol M. tomentosa extract can stimulate collagen type I, II, III and elastin creation through expression of COL1A , COL1A2 , COL2A1 , COL-II , COLL2 , COL3A and ELN genes; inhibit enzyme synthesis for collagen and elastin degradation through ELANE , MMP1 , MMP13 , TIMP1 genes; does not affect DHFa cells, but does affect CHL-1 cells through the NRF2 gene; can stimulate DHFa cell proliferation through the FGF1 and FGF7 genes; has anti-inflammatory effects on DHFa cells, and has no effects on CHL-1 cells through the TGF-β gene. Ethanol M. tomentosa extract can suppress melanin production through the α-MSH and TYR genes. In summary, M. tomentosa can be used in human health and skin care products, the research was accomplished with the aim of deducing how more plant species with high bioactivities can be utilized without toxicity, the leaves of M. tomentosa can be easier to use correctly than fruits depending on the season and smell.
{"title":"Biological activity of leaves of three Morinda species detected by stimulation and suppression of gene expression of collagen, elastin, melanin and other related genes","authors":"","doi":"10.35495/ajab.2023.117","DOIUrl":"https://doi.org/10.35495/ajab.2023.117","url":null,"abstract":"Morinda coreia , M. elliptica and M. tomentosa leaves were investigated. α-EG and pinoresinol were found by GC-MS and GC-FID. MTT and comet assays did not reveal toxicity in normal PBMCs and DHFa cells, but the ethanol and hexane M. coreia and M. tomentosa extracts were toxic to CHL-1 cancer cells. Biological activities were detected by gene expression through qRT-PCR. Ethanol M. tomentosa extract can stimulate collagen type I, II, III and elastin creation through expression of COL1A , COL1A2 , COL2A1 , COL-II , COLL2 , COL3A and ELN genes; inhibit enzyme synthesis for collagen and elastin degradation through ELANE , MMP1 , MMP13 , TIMP1 genes; does not affect DHFa cells, but does affect CHL-1 cells through the NRF2 gene; can stimulate DHFa cell proliferation through the FGF1 and FGF7 genes; has anti-inflammatory effects on DHFa cells, and has no effects on CHL-1 cells through the TGF-β gene. Ethanol M. tomentosa extract can suppress melanin production through the α-MSH and TYR genes. In summary, M. tomentosa can be used in human health and skin care products, the research was accomplished with the aim of deducing how more plant species with high bioactivities can be utilized without toxicity, the leaves of M. tomentosa can be easier to use correctly than fruits depending on the season and smell.","PeriodicalId":8506,"journal":{"name":"Asian Journal of Agriculture and Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135704914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of salinity stress on physiological aspects of pumpkin (Cucurbita moschata Duchesne. ‘Laikaotok’) under hydroponic condition 盐度胁迫对南瓜生理方面的影响。“莱卡托克”)在水培条件下
IF 2.2 Q2 Agricultural and Biological Sciences Pub Date : 2023-01-01 DOI: 10.35495/ajab.2021.01.050
Pumpkin ( Cucurbita moschata ) ‘Laikaotok’ is an important edible vegetable but growth and crop yield are affected by salinity stress. Response of physiological traits to salinity stress was investigated under hydroponic culture using Hoagland's solution at different NaCl concentrations of 0, 15, 30, 45, 60 and 120 mM. All treatments were cultured for four weeks and physiological traits were recorded. Results showed that pumpkin growth decreased after NaCl treatment, especially at 45 mM and higher concentrations. Leaf number, leaf width, leaf length, root number, stem length, stem diameter, SPAD unit, chlorophyll fluorescence in dark condition for 30 min (Fv'/Fm'), chlorophyll fluorescence in light condition (Fv/Fm), total chlorophyll, chlorophyll a and chlorophyll b contents significantly decreased (p < 0.05). Results indicated that salt concentration at less than 45 mM NaCl was suitable for C. moschata ‘Laikaotok’ growth. Phenotypic correlation coefficient estimation of 12 physiological traits showed positive correlation at a highly significant level, except for the correlation between plant height - Fv/Fm; plant height - Fv'/Fm'; root number - Fv/Fm and root number - Fv'/Fm'. This is the first report on salt stress response in C. moschata ‘Laikaotok’. Knowledge gained will be useful for pumpkin culture or rootstock selection under sensitive and moderately sensitive saline conditions.
{"title":"Effect of salinity stress on physiological aspects of pumpkin (Cucurbita moschata Duchesne. ‘Laikaotok’) under hydroponic condition","authors":"","doi":"10.35495/ajab.2021.01.050","DOIUrl":"https://doi.org/10.35495/ajab.2021.01.050","url":null,"abstract":"Pumpkin ( Cucurbita moschata ) ‘Laikaotok’ is an important edible vegetable but growth and crop yield are affected by salinity stress. Response of physiological traits to salinity stress was investigated under hydroponic culture using Hoagland's solution at different NaCl concentrations of 0, 15, 30, 45, 60 and 120 mM. All treatments were cultured for four weeks and physiological traits were recorded. Results showed that pumpkin growth decreased after NaCl treatment, especially at 45 mM and higher concentrations. Leaf number, leaf width, leaf length, root number, stem length, stem diameter, SPAD unit, chlorophyll fluorescence in dark condition for 30 min (Fv'/Fm'), chlorophyll fluorescence in light condition (Fv/Fm), total chlorophyll, chlorophyll a and chlorophyll b contents significantly decreased (p < 0.05). Results indicated that salt concentration at less than 45 mM NaCl was suitable for C. moschata ‘Laikaotok’ growth. Phenotypic correlation coefficient estimation of 12 physiological traits showed positive correlation at a highly significant level, except for the correlation between plant height - Fv/Fm; plant height - Fv'/Fm'; root number - Fv/Fm and root number - Fv'/Fm'. This is the first report on salt stress response in C. moschata ‘Laikaotok’. Knowledge gained will be useful for pumpkin culture or rootstock selection under sensitive and moderately sensitive saline conditions.","PeriodicalId":8506,"journal":{"name":"Asian Journal of Agriculture and Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69847407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Performance of broiler birds on feeding natural anti stressors in summer during heat stress 热应激条件下夏季饲喂天然抗应激物对肉鸡生产性能的影响
IF 2.2 Q2 Agricultural and Biological Sciences Pub Date : 2023-01-01 DOI: 10.35495/ajab.2022.024
This study was planned to assess the effectiveness of natural anti heat stressors on the broilers during hot climate. Three hundred one day old broiler birds were divided into four treatments groups, yoghurt, garlic powder and mint and a control group. After two weeks of rearing, data were recorded for growth performance, carcass and visceral organ yield and immune response against Newcastle Disease. Statistical analysis revealed that growth performance (weight gain, carcass weight and cumulative feed conversion ratio (FCR) was significantly improved with garlic powder, but feed intake remained non-significant. Dressing% and breast% were also significantly higher in garlic fed birds and similar effect was observed for intestinal length. Among visceral organs, liver% was improved upon garlic supplemented broiler but heart and gizzard were not influenced by dietary treatments. Additionally, garlic powder in feed also resulted in significantly higher antibody titer against Newcastle disease. It was concluded that garlic as natural anti-heat stressor has significant positive influence on the performance of heat stressed broilers.
{"title":"Performance of broiler birds on feeding natural anti stressors in summer during heat stress","authors":"","doi":"10.35495/ajab.2022.024","DOIUrl":"https://doi.org/10.35495/ajab.2022.024","url":null,"abstract":"This study was planned to assess the effectiveness of natural anti heat stressors on the broilers during hot climate. Three hundred one day old broiler birds were divided into four treatments groups, yoghurt, garlic powder and mint and a control group. After two weeks of rearing, data were recorded for growth performance, carcass and visceral organ yield and immune response against Newcastle Disease. Statistical analysis revealed that growth performance (weight gain, carcass weight and cumulative feed conversion ratio (FCR) was significantly improved with garlic powder, but feed intake remained non-significant. Dressing% and breast% were also significantly higher in garlic fed birds and similar effect was observed for intestinal length. Among visceral organs, liver% was improved upon garlic supplemented broiler but heart and gizzard were not influenced by dietary treatments. Additionally, garlic powder in feed also resulted in significantly higher antibody titer against Newcastle disease. It was concluded that garlic as natural anti-heat stressor has significant positive influence on the performance of heat stressed broilers.","PeriodicalId":8506,"journal":{"name":"Asian Journal of Agriculture and Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69849670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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Asian Journal of Agriculture and Biology
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