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Light-emitting diode enhances the biomass yield and antioxidant activity of Philippine wild mushroom Lentinus swartzii 发光二极管提高了菲律宾野生香菇的生物量和抗氧化活性
IF 2.2 Q2 Agricultural and Biological Sciences Pub Date : 2021-02-08 DOI: 10.35495/AJAB.2020.08.435
Reyna C. Tiniola
Lentinus swartzii is a basidiomycetous fungus that usually grows on fallen logs during rainy season. In this study, the effects of color light-emitting diode (LED) on the mycelial biomass, fruiting body production, and antioxidant properties of L. swartzii were evaluated. The inoculated culture bottles and fruiting bags were incubated under the three-color LEDs (red, green, and blue), and dark condition (control) in a chamber. Phenolic content and scavenging activity mycelia and fruiting bodies ethanol extracts were also analyzed. In liquid culture, red LED cultures produced the highest mycelial dry weight (0.464 g), while green LED cultures registered the highest number of primordia (6.0). The initiation of primordia was not stimulated under red LED and dark condition. However, in fruiting body production, fruiting bags exposed under red LED showed the shortest period of incubation for mycelial ramification (21.20 days), produced the maximum yield of fruiting body (35.73 g) and biological efficiency (7.14%). All LED mycelial extracts exhibited higher radical scavenging activities (RSA) than catechin, and green LED mycelial extract registered the highest phenolic content (PC) (34.21 mg GAE g sample). The red LED fruiting body extract recorded the highest RSA (28.06%) and PC (26.08 mg GAE g sample). Therefore, cultivation of L. swartzii in red LED chamber is a practical technique for enhancing biomass production and antioxidant properties.
香菇(Lentinus swartzii)是一种担子真菌,通常在雨季生长在倒下的原木上。研究了彩色发光二极管(LED)对水稻菌丝生物量、子实体产量和抗氧化性能的影响。接种后的培养瓶和果袋在三色led(红、绿、蓝)和暗色条件(对照)下在室内孵育。分析了菌丝和子实体乙醇提取物的酚含量和清除活性。在液体培养中,红色LED培养物产生的菌丝干重最高(0.464 g),而绿色LED培养物产生的原基数量最高(6.0)。在红色LED和黑暗条件下,原基的起始不受刺激。而在子实体生产中,红色LED照射下的子袋菌丝分枝培养时间最短(21.20 d),子实体产量最高(35.73 g),生物效率最高(7.14%)。所有LED菌丝体提取物的自由基清除活性(RSA)均高于儿茶素,其中绿色LED菌丝体提取物的酚含量(PC)最高(34.21 mg GAE g样品)。红色LED子实体提取物的RSA(28.06%)和PC (26.08 mg GAE g样品)最高。因此,在红色LED室内培养swartzii是一种提高生物质产量和抗氧化性能的实用技术。
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引用次数: 3
Identification of conserved and novel mature miRNAs in selected crops as future targets for metabolic engineering 在选定作物中鉴定保守和新的成熟miRNA作为代谢工程的未来靶点
IF 2.2 Q2 Agricultural and Biological Sciences Pub Date : 2021-02-01 DOI: 10.35495/AJAB.2020.12.551
Sidra Aslam
MicroRNAs (miRNAs) are small non-coding RNA molecules, involved in the posttranscriptional gene expression of countless metabolic pathways including plant biomass production. The current work was focused on identification of miRNAs involved in the growth metabolism of Glycine max, Oryza sativa, Zea mays, Sorghum bicolor, Brassica napus, Triticum aestivum. In order to identify conserved miRNA clusters, the miRNA data were collected from miRBase database. Overall, 756, 738, 325, 241, 92, and 125 datasets of the mature miRNA sequences of Glycine max, Oryza sativa, Zea mays, Sorghum bicolor, Brassica napus, Triticum aestivum were collected from miRbase. Using MEGA software, a total of 6, 6, 5, 6, and 3 conserved miRNA clusters were examined in aforementioned crops, respectively, with the aim of studying the conserved miRNA clusters belonging to same gene families. The conserved miRNA clusters were shown to belong to miR166, miR399, miR156, miR171, miR164, miR167, and miR394 families in the selected crops. This study may lead to elucidate the role of these miRNAs and their subsequent exploitation to enhance the biomass production via metabolic pathway engineering.
微小RNA(miRNA)是一种小的非编码RNA分子,参与无数代谢途径的转录后基因表达,包括植物生物量的产生。目前的工作重点是鉴定参与大豆、水稻、玉米、双色高粱、甘蓝型油菜、小麦生长代谢的miRNA。为了鉴定保守的miRNA簇,从miRBase数据库中收集miRNA数据。总体而言,从miRbase中收集了大豆、水稻、玉米、双色高粱、甘蓝型油菜、小麦的成熟miRNA序列的756、738、325、241、92和125个数据集。使用MEGA软件,在上述作物中分别检测了总共6、6、5、6和3个保守的miRNA簇,目的是研究属于相同基因家族的保守miRNA簇。保守的miRNA簇显示属于所选作物中的miR166、miR399、miR156、miR171、miR164、miR167和miR394家族。这项研究可能会阐明这些miRNA的作用,以及它们随后通过代谢途径工程提高生物量的利用。
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引用次数: 4
Performance of Italian ryegrass varieties: Effect of cutting stage on dry matter and total non-structural carbohydrates percentages 意大利黑麦草品种生产性能:刈割期对干物质和总非结构性碳水化合物百分比的影响
IF 2.2 Q2 Agricultural and Biological Sciences Pub Date : 2021-01-30 DOI: 10.35495/ajab.2020.02.116
.
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引用次数: 1
Using brown seaweed as a biofertilizer in the crop management industry and assessing the nutrient upliftment of crops 在作物管理行业中使用褐藻作为生物肥料并评估作物的营养提升
IF 2.2 Q2 Agricultural and Biological Sciences Pub Date : 2021-01-30 DOI: 10.35495/ajab.2020.04.257
G. Indika, P. Silva, Makawita, I. Wickramasinghe, I. Wijesekara
Due to the increasing levels of the world population, the demand for agricultural products has also increased over the years. Enhancing the yield and nutrient content of food products is therefore an important aspect in the current context. Experiments were conducted at both lab-scale and field level with extractions from Sargassum sp., to assess the growth of the plant, crop production, and nutrient content of Vigna radiata . A lab-scale experiment was conducted to identify and distinguish the germination percentage and seedling vigor of Sargassum treated and non-treated seeds. This included the application of a concentration series of polysaccharides and a concentration series of crude seaweed extract. The highest germination percentage was observed at 8% polysaccharide content and 15% liquid seaweed extraction (LSE) content resulting in 14% and 8% of germination increment against the control. When increasing the polysaccharide and LSE content exceeding the above-stated values, germination rate and seedling vigor dropped. When applied as foliar applications, an increment of pods against the control showed an increment of 28%, which is the maximum increment reached the 10% LSE concentration. With the polysaccharide application, the maximum yield increment was 31% with 10% concentration. Also, the dry weight content of seeds and the number of pods per plant and seed per pod have increased considerably. Further, both polysaccharide and LSE applications have increased the micronutrient and protein content in seeds. Therefore, it is recommended to use the LSE at 15% concentration and polysaccharide at 8% concentration at the germination stage and as a foliar application to increase the yield and nutrient content of the Vigna radiata plant.
由于世界人口水平的不断提高,多年来对农产品的需求也有所增加。因此,提高食品的产量和营养成分是当前形势下的一个重要方面。用马尾藻提取物在实验室规模和田间水平上进行了实验,以评估植物的生长、作物产量和Vigna radiata的营养成分。通过室内试验,对马尾藻处理和未处理种子的发芽率和幼苗活力进行了鉴定和区分。这包括多糖浓度系列和海藻粗提取物浓度系列的应用。在多糖含量为8%和液体海藻提取物(LSE)含量为15%时,发芽率最高,与对照相比,发芽率分别提高了14%和8%。当多糖和LSE含量超过上述值时,发芽率和幼苗活力下降。当以叶面施用时,与对照相比,荚的增量显示出28%的增量,这是达到10%LSE浓度的最大增量。随着多糖的施用,当浓度为10%时,最大产量增加31%。此外,种子的干重含量以及每株植物和每荚种子的荚数也显著增加。此外,多糖和LSE的应用都增加了种子中的微量营养素和蛋白质含量。因此,建议在发芽阶段和叶面施用15%浓度的LSE和8%浓度的多糖,以提高Vigna radiata植物的产量和营养成分。
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引用次数: 14
A wheat stress induced WRKY transcription factor TaWRKY32 confers drought stress tolerance in Oryza sativa 小麦胁迫诱导的WRKY转录因子TaWRKY32赋予水稻抗旱性
IF 2.2 Q2 Agricultural and Biological Sciences Pub Date : 2021-01-30 DOI: 10.35495/ajab.2020.06.371
E. Darwish
WRKY transcription factors group play a significant role in stress signalling pathway. In this study, a WRKY member of bread wheat, TaWRKY , was cloned and its function in response to water stress conditions was studied. Expression analysis revealed that TaWRKY32 mainly expressed when plants were subjected to PEG, NaCl, and cold and probably participate in ABA dependent signalling pathways. This study was done to elucidate the role in transgenic breeding, TaWRK32 was transferred to rice plants under CaMV-35S promoter. Overexpression of TaWRK32 lead to enhanced fresh shoot and root weight at seedling stage when compared to wild type (WT). Moreover, transgenic rice lines under water stress, showed greater recovery rate when compared to WT. Higher values of cell membrane stability and lower Chl a/b ratio of transgenic rice lines under water stress conditions markedly indicated the importance of TaWRKY32 under water stress conditions. These results suggested that TaWRK32 has multifunctional and might be an instrumental tool in breeding for transgenic plants for improved water stress tolerance in wheat.
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引用次数: 7
Predicting biogas production using kinetic model for mesophilic and thermophilic temperature regimes: a theoretical approach 使用中温和高温温度条件下的动力学模型预测沼气产量:一种理论方法
IF 2.2 Q2 Agricultural and Biological Sciences Pub Date : 2021-01-30 DOI: 10.35495/ajab.2020.03.196
Ever since global warming, largely caused by consumption of fossil fuels, has alarmed the scientists and policy makers, biomass has been gaining acceptance and currency as a means of sustainable energy generation. Biogas generation through anaerobic digestion of biomass is a promising technique of converting carbonaceous material into methane and carbon dioxide i.e major components of biogas. This work aims at determination of methane production rate based on kinetic study while taking into consideration temperature, total solids, volatile solids, residence time, and bacterial growth. We used banana waste including stem, fruit stem, peel and leaves as waste material and applied Chen & Hashimoto kinetic model to measure the methane potential. The wet anaerobic digestion process digested the banana waste inside the bioreactor for 15 days of hydraulic retention time on mesophilic temperature regime 35-40°C and 10 days for thermophilic temperature regime 55-60°C. The methane production was 14.6 m 3 /day using 100 kg/day of banana waste. This showed a close accord to the already observed and published data based on total solid, volatile solid, carbon to nitrogen ratio and temperature.
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引用次数: 0
Recent advances in molecular characterization of Sarcocystis species in some meat producing animals: an updated review 肉制品中肉孢子虫分子特征研究进展
IF 2.2 Q2 Agricultural and Biological Sciences Pub Date : 2021-01-30 DOI: 10.35495/ajab.2020.09.502
Sarcocystosis is a parasitic disease caused by Sarcocystis species that infect humans and animals. It is prevalent in small ruminants like sheep and goats worldwide and causing pathogenic impacts that lead to economic losses owing to carcass condemnation, abortion, and death. Recently, several molecular and phylogenetic analyses have been developed to differentiate Sarcocystis species including, the 18S rRNA, 28S rRNA, 18S rDNA, and ITS-1 region. In recent years, the mitochondrial cytochrome c oxidase subunit 1 (cox-1) was successfully used for this purpose. The DNA barcoding using the cox 1 gene is a reliable tool to distinguish and identify the main Sarcocystis genotypes. Therefore, several studies confirmed that the cox 1 gene is a promising DNA marker for studying the genus Sarcocystis . The current review aims to highlight the molecular methods that exist for the identification of Sarcocystis species . The results showed that the Sarcocystis species of sheep and goats were genetically close related and may be considered as sibling strains, as well as the cross-infection may happen among them. Consequently, the host specificity of several Sarcocystis species is questionable. The findings additional emphasized that experimental transmission investigations within the proposed definitive host are required to confirm the characteristics and host ranges of the Sarcocystis spp. in sheep and goats. The current review represents updated knowledge about molecular discrimination of Sarcocystis species in small ruminants by reviewing and analyzing the recent articles in this aspect.
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引用次数: 5
Effect of organic fertilization on quality and yield of oriental tobacco (Nicotiana tabacum L.) under Mediterranean conditions 地中海条件下有机施肥对烟草品质和产量的影响
IF 2.2 Q2 Agricultural and Biological Sciences Pub Date : 2021-01-30 DOI: 10.35495/ajab.2020.05.274
Ioanna Tabaxi
Greece is a country that primarily cultivates tobacco. Over the years and the increase of inputs to conventional tobacco cultivation and the groundwater nitrate pollution, producers and industry began to look at organic farming. However, for the field crop of tobacco knowledge is limited to inorganic fertilization only and it is necessary now to find out the effect of organic fertilization on soil, yield and quality of tobacco. Therefore, two-year field experiments were carried out during 2017-2018 in Agricultural University of Athens. Three oriental tobacco varieties were cultivated (Basmas Xanthi 81, Katerini S53 and Myrodata Agriniou 30Α) in split-plot design, with four fertilization treatments (Control, Compost, Manure and NPK). According to the results, the yield was significantly affected only by the variety and the highest yield was 3.488 (kg ha-1) in Myrodata Agriniou variety. The plant height was significantly affected by variety and ranged from 110,77 cm (Basmas Xanthi) to 121.42 cm (Myrodata Agriniou). The number of leaves was significantly affected only by the variety; Katerini S53 had the highest number. The nicotine content was significantly affected by variety and year and ranged between 2.52% (Myrodata Agriniou) and 4.22 % (Basmas Xanthi). As nicotine increases, sugars finally decrease with the application of organic fertilizers. Summarizing, most of the soil characteristics significantly affected by the fertilization and showed generally improvement. The plant growth and the final yield was determined by the variety. With organic tobacco cultivation, the quality and yields of oriental varieties have improved significantly.
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引用次数: 15
Nutritional and physical requirements for mycelial growth and basidiocarp production of Trametes elegans from the Philippines 菲律宾秀丽曲线虫菌丝生长和担子果生产的营养和生理要求
IF 2.2 Q2 Agricultural and Biological Sciences Pub Date : 2021-01-30 DOI: 10.35495/ajab.2020.06.339
R. M. Dulay
This paper highlights the rescue and optimal cultural conditions of the naturally-occurring Trametes elegans . This mushroom has white fan-like leathery fruiting body that commonly grows singly on deadwood. The secondary mycelial culture has off-white, velvety texture
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引用次数: 6
Exposure to heavy metals causes histopathological changes and alters antioxidant enzymes in fresh water fish (Oreochromis niloticus) 暴露于重金属会导致淡水鱼(Oreochromis niloticus)的组织病理学变化并改变抗氧化酶
IF 2.2 Q2 Agricultural and Biological Sciences Pub Date : 2021-01-30 DOI: 10.35495/ajab.2020.03.143
Tuba Rehman
Expansion in technology, development in industries and extensive applications of synthetic chemical have become major regulatory issues toward the public health, wildlife and aquatic ecosystems. Therefore, the monitoring and investigation of environmental contaminants in order to mitigate the adverse impacts is crucial to save public health and aquatic wildlife. The present study describes the deleterious effects of heavy metals at low levels in fresh water fish. A total of 120 fish of same age and body mass were arbitrarily distributed and allocated into eight groups (A-H). Group A was run parallel to other groups and maintained as control group. Collected data was analyzed by using Tukey’s Test. Results indicated various clinical ailments including erratic swimming and jerking movement. Microscopic observation of gills, liver and brain tissues showed different histopathological changes in fish exposed to various heavy metals. The antioxidant enzymes activities were increased or decreased in a concentration and enzyme dependent manner. Exposure of treatments showed significant increase in peroxidase and glutathione-s-transferase but decreased catalase and superoxide dismutase activity at higher exposure to heavy metals. Regarding overall toxicity, nickel was least sensitive towards the fish, but tertiary metal mixture was more toxic to fish (Oreochromis niloticus) followed by binary mixture. In conclusion, results of our study suggested that the exposure to different heavy metals even at low concentrations poses serious concern towards their potential danger to the survival and growth of fish and induces deleterious effects in gills, brain and liver tissues.
合成化学品的技术扩张、工业发展和广泛应用已成为公共卫生、野生动物和水生生态系统的主要监管问题。因此,监测和调查环境污染物以减轻不利影响对于保护公众健康和水生野生动物至关重要。本研究描述了低水平重金属对淡水鱼类的有害影响。共有120条年龄和体重相同的鱼被任意分配并分为八组(A-H)。A组与其他组平行进行,并维持为对照组。采用Tukey检验对收集的数据进行分析。结果表明,各种临床疾病包括不稳定的游泳和急动。对暴露于各种重金属的鱼的鳃、肝和脑组织的显微镜观察显示出不同的组织病理学变化。抗氧化酶活性以浓度和酶依赖的方式增加或降低。暴露于重金属的处理显示过氧化物酶和谷胱甘肽-s-转移酶显著增加,但过氧化氢酶和超氧化物歧化酶活性降低。就总体毒性而言,镍对鱼类的敏感性最低,但三元金属混合物对鱼类的毒性更大(尼罗罗非鱼),其次是二元混合物。总之,我们的研究结果表明,即使在低浓度下,接触不同的重金属也会对鱼类的生存和生长造成严重威胁,并对鳃、脑和肝组织产生有害影响。
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引用次数: 17
期刊
Asian Journal of Agriculture and Biology
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