Pub Date : 2017-10-26DOI: 10.7904/2068-4738-VIII(16)-39
M. Menkovska, D. Damjanovski, V. Levkov, N. Gjorgovska, D. Knežević, N. Nikolova, V. Stanoev
3065120 Abstract . In this paper, the results obtained from the investigations performed on determination of the content of soluble dietary fibre component β –glucan in different cereals grown by conventional and organic farming method are presented. Because dietary fibre and its components have great importance for human and animal nutrition as well as health benefits, it was a need to investigate the content of β– glucan from cereal domestic origin. Standardized enzymatic–gravimetric method–a mixed–linkage β –glucan assay Megazyme Kit (Megazyme, Ireland) was used for determination of β –glucan content in 27 different cereal samples: barley, oat, rye and wheat, grown at three locations during 2013. The mean values of β– glucan content in cereals conventionally grown ranged from 34.41 ± 1.47 g.kg –1 db with barley, through 23.5 ± 4.52 g.kg –1 db and 15.61 ± 0.36 g.kg –1 db with oat and rye, respectively to 5.60 ± 1.02 g.kg –1 db with wheat. The mean values of β– glucan content in analyzed cereals which were organically grown ranged from 33.28 ± 2.34 g.kg –1 db with barley, 21.3 ± 1.87 g.kg –1 db with oat, 15.81 ± 2.30 g.kg –1 db with rye and to 6.04 ± 0.58 g.kg –1 db with wheat. Compared to the other cereals investigated, barley grown by either conventional or organic farming method proved to have the highest content of β –glucan demonstrating its good perspective in cereal processing and technology, food market and human and animal nutrition. The positive/negative correlations allow indirect evaluation of cereals in breeding work aimed at creation of forms with increased/decreased content of β– glucans in
{"title":"Content of Β–glucan in cereals grown by organic and conventional farming","authors":"M. Menkovska, D. Damjanovski, V. Levkov, N. Gjorgovska, D. Knežević, N. Nikolova, V. Stanoev","doi":"10.7904/2068-4738-VIII(16)-39","DOIUrl":"https://doi.org/10.7904/2068-4738-VIII(16)-39","url":null,"abstract":"3065120 Abstract . In this paper, the results obtained from the investigations performed on determination of the content of soluble dietary fibre component β –glucan in different cereals grown by conventional and organic farming method are presented. Because dietary fibre and its components have great importance for human and animal nutrition as well as health benefits, it was a need to investigate the content of β– glucan from cereal domestic origin. Standardized enzymatic–gravimetric method–a mixed–linkage β –glucan assay Megazyme Kit (Megazyme, Ireland) was used for determination of β –glucan content in 27 different cereal samples: barley, oat, rye and wheat, grown at three locations during 2013. The mean values of β– glucan content in cereals conventionally grown ranged from 34.41 ± 1.47 g.kg –1 db with barley, through 23.5 ± 4.52 g.kg –1 db and 15.61 ± 0.36 g.kg –1 db with oat and rye, respectively to 5.60 ± 1.02 g.kg –1 db with wheat. The mean values of β– glucan content in analyzed cereals which were organically grown ranged from 33.28 ± 2.34 g.kg –1 db with barley, 21.3 ± 1.87 g.kg –1 db with oat, 15.81 ± 2.30 g.kg –1 db with rye and to 6.04 ± 0.58 g.kg –1 db with wheat. Compared to the other cereals investigated, barley grown by either conventional or organic farming method proved to have the highest content of β –glucan demonstrating its good perspective in cereal processing and technology, food market and human and animal nutrition. The positive/negative correlations allow indirect evaluation of cereals in breeding work aimed at creation of forms with increased/decreased content of β– glucans in","PeriodicalId":8682,"journal":{"name":"Banat's Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74513246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-10-26DOI: 10.7904/2068-4738-VIII(16)-116
A. Dadkhah, A. E. Rad, R. Azizinezhad
Reducing fat and calorie is an important priority in optimization of bakery products including high–fat cakes. The aim of this research was to evaluate the effects of shortening replacement with pumpkin powder at 0 %, 20 % and 40 % with or without water level increment on rheological properties of cake batter and two properties of produced cake. Pumpkin powder was obtained from pumpkin flesh through drying, grinding and sieving processes. The rheological attributes of the batter and the specific volume and moisture content of cakes were examined using a rheometer, rapeseed displacement method and air–oven method, respectively. Increasing shortening replacement, enhanced viscosity (87.00 Pa.s), while increasing water in shortening–replaced treatments reduced it (11.30 Pa.s). Samples showed shear thinning behavior. In linear viscoelastic range, samples indicated solid viscoelastic behavior. Loss tangent, storage and loss moduli obtained from frequency sweep test, were investigated in three frequencies. In each frequency, with increasing shortening replacement, their viscoelastic properties enhanced. Contrary, increasing water amount in shortening reduced–batter samples, weakened system structuring. With increasing frequency, both moduli of treatments increased and the loss tangent of 20 % reduced–shortening cake without water level increment showed a more similar trend to the control (0 % replacement without increasing water amount). The moisture content of cakes containing pumpkin was greater than control. The specific volume of cake with 20 % replacement without additional water (2.68 cm/g) was similar to the control and higher than other shortening– replaced treatments. Therefore, this treatment was chosen as the best reduced–fat cake. Keyword: Cake batter, Pumpkin, Rheological properties, Shortening replacement, Specific volume.
{"title":"Effect of pumpkin powder as a fat replacer on rheological properties, specific volume and moisture content of cake","authors":"A. Dadkhah, A. E. Rad, R. Azizinezhad","doi":"10.7904/2068-4738-VIII(16)-116","DOIUrl":"https://doi.org/10.7904/2068-4738-VIII(16)-116","url":null,"abstract":"Reducing fat and calorie is an important priority in optimization of bakery products including high–fat cakes. The aim of this research was to evaluate the effects of shortening replacement with pumpkin powder at 0 %, 20 % and 40 % with or without water level increment on rheological properties of cake batter and two properties of produced cake. Pumpkin powder was obtained from pumpkin flesh through drying, grinding and sieving processes. The rheological attributes of the batter and the specific volume and moisture content of cakes were examined using a rheometer, rapeseed displacement method and air–oven method, respectively. Increasing shortening replacement, enhanced viscosity (87.00 Pa.s), while increasing water in shortening–replaced treatments reduced it (11.30 Pa.s). Samples showed shear thinning behavior. In linear viscoelastic range, samples indicated solid viscoelastic behavior. Loss tangent, storage and loss moduli obtained from frequency sweep test, were investigated in three frequencies. In each frequency, with increasing shortening replacement, their viscoelastic properties enhanced. Contrary, increasing water amount in shortening reduced–batter samples, weakened system structuring. With increasing frequency, both moduli of treatments increased and the loss tangent of 20 % reduced–shortening cake without water level increment showed a more similar trend to the control (0 % replacement without increasing water amount). The moisture content of cakes containing pumpkin was greater than control. The specific volume of cake with 20 % replacement without additional water (2.68 cm/g) was similar to the control and higher than other shortening– replaced treatments. Therefore, this treatment was chosen as the best reduced–fat cake. Keyword: Cake batter, Pumpkin, Rheological properties, Shortening replacement, Specific volume.","PeriodicalId":8682,"journal":{"name":"Banat's Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78159593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-09-20DOI: 10.7904/2068-4738-VIII(16)-62
N. Georgieva, I. Nikolova
An important tendency over the past years in the conventional cultivation of crops is the reduced use of synthetic products. The experiment was carried out in the period 2014– 2015 at the Institute of forage crops (Pleven) and aimed to study the biological performances of alfalfa after application of mineral oil Akarzin, either alone and in combination with reduced doses of synthetic insecticide (Eforia 045 ZC). The alone and combined use of Akarzin had no phytotoxic effect on the crop and influenced positively on the relative water content and dry mass productivity (an increase by 5.2 to 8.7 % and by 10.1 to 29.2 %, respectively). The treatment with the synthetic products also had a favorable effect on the phytosanitary status of the crop and restricted the аttack by Pseudopeziza medicaginis. The combined application of Akarzin along with Eforia 045 ZC allowed reducing the applied dose of the insecticide up to 0.03 and 0.01 %, as the same time the achieved effect in regard to the productivity exceeded the one of self–application of Eforia 045 ZC in the highest dose (0.05 %). With a view to reducing the negative impact on environment a reduction of the application dose of the insecticide after combining it with the mineral oil is recommended as an environmentally friendly approach in conditions of conventional cultivation of alfalfa. Keyword: mineral oil, alfalfa, reduced doses, yield.
{"title":"Biological performances of alfalfa treated with mineral oil Akarzin and reduced doses of insecticide","authors":"N. Georgieva, I. Nikolova","doi":"10.7904/2068-4738-VIII(16)-62","DOIUrl":"https://doi.org/10.7904/2068-4738-VIII(16)-62","url":null,"abstract":"An important tendency over the past years in the conventional cultivation of crops is the reduced use of synthetic products. The experiment was carried out in the period 2014– 2015 at the Institute of forage crops (Pleven) and aimed to study the biological performances of alfalfa after application of mineral oil Akarzin, either alone and in combination with reduced doses of synthetic insecticide (Eforia 045 ZC). The alone and combined use of Akarzin had no phytotoxic effect on the crop and influenced positively on the relative water content and dry mass productivity (an increase by 5.2 to 8.7 % and by 10.1 to 29.2 %, respectively). The treatment with the synthetic products also had a favorable effect on the phytosanitary status of the crop and restricted the аttack by Pseudopeziza medicaginis. The combined application of Akarzin along with Eforia 045 ZC allowed reducing the applied dose of the insecticide up to 0.03 and 0.01 %, as the same time the achieved effect in regard to the productivity exceeded the one of self–application of Eforia 045 ZC in the highest dose (0.05 %). With a view to reducing the negative impact on environment a reduction of the application dose of the insecticide after combining it with the mineral oil is recommended as an environmentally friendly approach in conditions of conventional cultivation of alfalfa. Keyword: mineral oil, alfalfa, reduced doses, yield.","PeriodicalId":8682,"journal":{"name":"Banat's Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88154953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-05-20DOI: 10.7904/2068-4738-VIII(15)-12
V. Jovanovska, Z. Sovreski, M. Arapceska, G. Dimitrovska, K. Bojkovska, Elena Joshevska, Katerina Jovanovska
{"title":"DEVICES FOR BACTOFUGATION IN THE FUNCTION OF PROVIDING QUALITY LONG–LIFE MILK","authors":"V. Jovanovska, Z. Sovreski, M. Arapceska, G. Dimitrovska, K. Bojkovska, Elena Joshevska, Katerina Jovanovska","doi":"10.7904/2068-4738-VIII(15)-12","DOIUrl":"https://doi.org/10.7904/2068-4738-VIII(15)-12","url":null,"abstract":"","PeriodicalId":8682,"journal":{"name":"Banat's Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82135260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-05-20DOI: 10.7904/2068-4738-VIII(15)-25
S. Hassan, Zahra Belb Belbasi
Plants have been considered as a significant source of drugs since ancient times. Secondary metabolites derived from plants have revealed therapeutic properties. Artemisinin and its derivatives are the most famous terpenoids in the Asteraceae family. Nowadays, numerous attempts have been done to improve the production rate of these kinds of terpenoids in biotechnological procedures which most of them are allocated to hairy root cultures using Agrobacterium rhizogenes (A4, A7, Ar15834, Ar9534, Ar9402, Ar318 and Ar50). In this study, we tried to investigate the effect different strains of A. rhizogenes on frequency of hairy root induction in A. annua. Explants were prepared from leaves of A. annua. Then they were soaked in A. rhizogenes solution to be infected. They were cultured in MS medium for 14 days. For decreasing plant necrosis, two different concentrations of 0.1 and 0.5 % were added to the medium as anti– oxidant. For hairy root induction approve, PCR method was applied. Hairy roots were obvious in plantlets on 1⁄2 MS medium culture. The presence of T–DNA in different transformed lines of hairy roots was investigated by PCR. The results indicated that making 0.5 % ascorbic acid MS Medium will lead to a dramatic decrease (about 80 %) in plantlet’s browning. In addition, the highest and lowest transformation rate of 79 % and 24 % were yielded, infecting the leaf explants of A. annua by A7 and Ar318 strains respectively. To sum it up, it was shown that all mentioned strains are capable to induce hairy root in A. annua by the help of ascorbic acid as an inhibitor for plantlet’s browning. Because of hormone–autotrophic feature and great lateral branches of hairy roots, the induced hairy roots of A. annua can be applied to increase artemisinin production in pharmaceutical industry. Keyword: Artemisia annua; Agrobacterium rhizogenes; artemisinin; hairy root.
{"title":"IMPROVEMNET OF HAIRY ROOT INDUCTION IN Artemisia annua BY VARIOUS STRAINS OF Agrobacterium rhizogenes","authors":"S. Hassan, Zahra Belb Belbasi","doi":"10.7904/2068-4738-VIII(15)-25","DOIUrl":"https://doi.org/10.7904/2068-4738-VIII(15)-25","url":null,"abstract":"Plants have been considered as a significant source of drugs since ancient times. Secondary metabolites derived from plants have revealed therapeutic properties. Artemisinin and its derivatives are the most famous terpenoids in the Asteraceae family. Nowadays, numerous attempts have been done to improve the production rate of these kinds of terpenoids in biotechnological procedures which most of them are allocated to hairy root cultures using Agrobacterium rhizogenes (A4, A7, Ar15834, Ar9534, Ar9402, Ar318 and Ar50). In this study, we tried to investigate the effect different strains of A. rhizogenes on frequency of hairy root induction in A. annua. Explants were prepared from leaves of A. annua. Then they were soaked in A. rhizogenes solution to be infected. They were cultured in MS medium for 14 days. For decreasing plant necrosis, two different concentrations of 0.1 and 0.5 % were added to the medium as anti– oxidant. For hairy root induction approve, PCR method was applied. Hairy roots were obvious in plantlets on 1⁄2 MS medium culture. The presence of T–DNA in different transformed lines of hairy roots was investigated by PCR. The results indicated that making 0.5 % ascorbic acid MS Medium will lead to a dramatic decrease (about 80 %) in plantlet’s browning. In addition, the highest and lowest transformation rate of 79 % and 24 % were yielded, infecting the leaf explants of A. annua by A7 and Ar318 strains respectively. To sum it up, it was shown that all mentioned strains are capable to induce hairy root in A. annua by the help of ascorbic acid as an inhibitor for plantlet’s browning. Because of hormone–autotrophic feature and great lateral branches of hairy roots, the induced hairy roots of A. annua can be applied to increase artemisinin production in pharmaceutical industry. Keyword: Artemisia annua; Agrobacterium rhizogenes; artemisinin; hairy root.","PeriodicalId":8682,"journal":{"name":"Banat's Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82094345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-05-20DOI: 10.7904/2068-4738-VIII(15)-66
Nader Fall Anarjan, Gholamali Moghaddam, Y. Ebrahimnezhad, S. Aharizad
{"title":"EFFECTS OF MILK PRODUCTION AND SOME BLOOD METABOLITES ON PREGNANCY STABILITY IN LACTATING HOLSTEIN DAIRY COWS","authors":"Nader Fall Anarjan, Gholamali Moghaddam, Y. Ebrahimnezhad, S. Aharizad","doi":"10.7904/2068-4738-VIII(15)-66","DOIUrl":"https://doi.org/10.7904/2068-4738-VIII(15)-66","url":null,"abstract":"","PeriodicalId":8682,"journal":{"name":"Banat's Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82382532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-05-20DOI: 10.7904/2068-4738-VIII(15)-34
T. Bozhanska
{"title":"STUDY ON PERENNIAL LEGUME-GRASS MIXTURES IN THE CONDITIONS OF THE CENTRAL BALKAN MOUNTAIN","authors":"T. Bozhanska","doi":"10.7904/2068-4738-VIII(15)-34","DOIUrl":"https://doi.org/10.7904/2068-4738-VIII(15)-34","url":null,"abstract":"","PeriodicalId":8682,"journal":{"name":"Banat's Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84143139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-05-20DOI: 10.7904/2068-4738-VIII(15)-78
Hamza Belkhodja, A. Belmimoun, B. Meddah
{"title":"CHEMICAL CHARACTERIZATION OF POLYPHENOLS EXTRACTED FROM DIFFERENT HONEYS","authors":"Hamza Belkhodja, A. Belmimoun, B. Meddah","doi":"10.7904/2068-4738-VIII(15)-78","DOIUrl":"https://doi.org/10.7904/2068-4738-VIII(15)-78","url":null,"abstract":"","PeriodicalId":8682,"journal":{"name":"Banat's Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82853860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-05-20DOI: 10.7904/2068-4738-VIII(15)-50
M. Yadegari
The present study was conducted to investigate the in vivo to assess the effect of ostrich egg white (OEW)–derived graft substitutes on bone healing and comparative with self– regeneration of control site in dogs. The eight male domestic dogs (3–4 years old, weighing 20–30 kg) were used. Bone formation was assessed by using of OEW as kind of biomaterial that has flexibility and jelly shape with high compatibility. The implant was harvested after 4 and 8 weeks, and the area of new bone formation was quantified by radiology and densitometry studies. The highest bone formation was achieved with the OEW treatment, and it was significantly higher than that achieved with the empty treatment. The quantity of new bone at end of 8 weeks was greater than at 4 weeks in treated group. The researcher also show that Using OEW, the bone regeneration significantly increased within the implanted site in the biomaterial group, in the second month of the experiment, when compared to the control group. Furthermore, bone regeneration was significantly higher in the implant treatment biomaterial site of individual OEW, after implantation times, it’s showed little effect in enhancing bone regeneration 4 weeks post– implantation. Overall, it can be concluded that OEW (safe, cheap, and easily available) is a potent osteoidinductive component that plays critical role during bone regeneration and repairing in animal model. Keyword: Animal model, Bone regeneration, Implant, Ostrich egg, Tibia bone.
{"title":"EVALUATION OF BONE REGENERATION BY OSTRICH EGG WHITE SUBSTITUTE IMPLANTED WITH BONE IN TIBIA BONE DEFECT IN ANIMAL MODEL","authors":"M. Yadegari","doi":"10.7904/2068-4738-VIII(15)-50","DOIUrl":"https://doi.org/10.7904/2068-4738-VIII(15)-50","url":null,"abstract":"The present study was conducted to investigate the in vivo to assess the effect of ostrich egg white (OEW)–derived graft substitutes on bone healing and comparative with self– regeneration of control site in dogs. The eight male domestic dogs (3–4 years old, weighing 20–30 kg) were used. Bone formation was assessed by using of OEW as kind of biomaterial that has flexibility and jelly shape with high compatibility. The implant was harvested after 4 and 8 weeks, and the area of new bone formation was quantified by radiology and densitometry studies. The highest bone formation was achieved with the OEW treatment, and it was significantly higher than that achieved with the empty treatment. The quantity of new bone at end of 8 weeks was greater than at 4 weeks in treated group. The researcher also show that Using OEW, the bone regeneration significantly increased within the implanted site in the biomaterial group, in the second month of the experiment, when compared to the control group. Furthermore, bone regeneration was significantly higher in the implant treatment biomaterial site of individual OEW, after implantation times, it’s showed little effect in enhancing bone regeneration 4 weeks post– implantation. Overall, it can be concluded that OEW (safe, cheap, and easily available) is a potent osteoidinductive component that plays critical role during bone regeneration and repairing in animal model. Keyword: Animal model, Bone regeneration, Implant, Ostrich egg, Tibia bone.","PeriodicalId":8682,"journal":{"name":"Banat's Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82425748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-05-20DOI: 10.7904/2068-4738-VIII(15)-113
A. Ruchin
The objective of this work consists in valuing the carob pods (very rich in sugars and in nourishing elements) as being a medium of culture for growth of Lactobacillus bulgaricus and comparison this natural syrup with MRS medium for lactic acid production curve. By its biochemical composition, the carob syrup is very rich in carbohydrates 16 g/L, protein 0.25 %, ash 0.8 % including potassium (110 mg/100 mL of MF), sodium (80) and calcium (150). Kinetics study of growth of Lactobacillus bulgaricus in MRS and carob pods syrup showed that high biomass and lactic acid were obtained in the end of carob fermentations respectively (5.51 and 13.8) compared to MRS fermentation (4.57 and 10.9). This study clearly showed that carob pods have potential to be used as feedstock for lactic acid production by Lactobacillus bulgaricus using batch culture. Keyword: Abiotic Factor; Common Frog; Common Newt; Crested Newt; Development; Larvae; Moor Frog; Mortality.
{"title":"THE EFFECTS OF ILLUMINATION ON THE EARLY DEVELOPMENT OF TAILED AND TAILLESS AMPHIBIANS","authors":"A. Ruchin","doi":"10.7904/2068-4738-VIII(15)-113","DOIUrl":"https://doi.org/10.7904/2068-4738-VIII(15)-113","url":null,"abstract":"The objective of this work consists in valuing the carob pods (very rich in sugars and in nourishing elements) as being a medium of culture for growth of Lactobacillus bulgaricus and comparison this natural syrup with MRS medium for lactic acid production curve. By its biochemical composition, the carob syrup is very rich in carbohydrates 16 g/L, protein 0.25 %, ash 0.8 % including potassium (110 mg/100 mL of MF), sodium (80) and calcium (150). Kinetics study of growth of Lactobacillus bulgaricus in MRS and carob pods syrup showed that high biomass and lactic acid were obtained in the end of carob fermentations respectively (5.51 and 13.8) compared to MRS fermentation (4.57 and 10.9). This study clearly showed that carob pods have potential to be used as feedstock for lactic acid production by Lactobacillus bulgaricus using batch culture. Keyword: Abiotic Factor; Common Frog; Common Newt; Crested Newt; Development; Larvae; Moor Frog; Mortality.","PeriodicalId":8682,"journal":{"name":"Banat's Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79730292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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