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Axillary shoot proliferation and regeneration of red rose [pisiformis (christ.) D. Sosn. An under thread extinction species endemic to Turkey 红玫瑰[pisiformis (christ.) D. Sosn.土耳其特有的濒危物种
IF 0.3 4区 生物学 Q4 PLANT SCIENCES Pub Date : 2023-12-31 DOI: 10.3329/bjb.v52i4.70576
Onur Özel, Hatice Demiray, Volkan Eroğlu, Cihan Ecer
In vitro propagation of red rose [Rosa pisiformis (Christ.) D. Sosn.] has great importance in rapid proliferation of species with medicinal features and in culture of healthy plants free from deseases.  After a succesfully pre-sterilization procedure, experiments were maintained by two-phase: multiplication phase and rooting of microshoots phase. Micropropagation of Rose was improved, using its nodal segments under different combination of BAP (0, 1.0, 2.0, 0.6 mg/l), NAA (0, 0.1, 0.2, 0.01 mg/l) on Driver and Kuniyuki (DKW) and MS medium in multiplication phase and using distinct compositions of IBA (0, 1.0, 3.0, 5.0 mg/l) and DKW medium in rooting phase.  The maximum number of shoots/per plant (22.6) with the highest number of leaves (222.6) were obtained in DKW medium supplemented with 0.6 mgl-1 BAP and 0.01 mg/l NAA. The leaf explants were also used to occur callus in MS with different combinations of BAP and NAA. Good callus formation was obtained with 1:2 ratio of BAP:NAA combination in MS medium. Furthermore, the highest root induction (100%) was achieved in DKW medium consisting of 1 mg/l  IBA. The rooted plantlets were transferred to the field after acclimatized in greenfield conditions. The present investigation presents an in vitro protocol for R. pisiformis. Bangladesh J. Bot. 52(4): 949-958, 2023 (December)
红玫瑰[Rosa pisiformis (Christ.) D. Sosn.]的体外繁殖对于快速增殖具有药用价值的品种和培养无病虫害的健康植株具有重要意义。 经过成功的预灭菌程序后,实验分两个阶段进行:繁殖阶段和小芽生根阶段。在增殖阶段,使用不同组合的 BAP(0、1.0、2.0、0.6 毫克/升)、NAA(0、0.1、0.2、0.01 毫克/升)在 Driver 和 Kuniyuki(DKW)和 MS 培养基上进行微繁殖,在生根阶段,使用不同组合的 IBA(0、1.0、3.0、5.0 毫克/升)和 DKW 培养基进行微繁殖。 在添加了 0.6 毫克/升 BAP 和 0.01 毫克/升 NAA 的 DKW 培养基中,每株获得的芽数最多(22.6),叶片数最多(222.6)。叶片外植体也被用来在添加了不同 BAP 和 NAA 组合的 MS 中形成胼胝体。在 MS 培养基中,BAP:NAA 的组合比例为 1:2,胼胝体形成效果良好。此外,在含有 1 毫克/升 IBA 的 DKW 培养基中,根诱导率最高(100%)。生根的小植株在绿地条件下适应后被转移到田间。本研究提出了一种 R. pisiformis 的离体培养方案。孟加拉国植物学杂志》(Bangladesh J. Bot.52(4):949-958,2023 年(12 月)
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引用次数: 0
Karyotype profile of holarrhena antidysenterica (Roxb. Ex fleming) wall holarrhena antidysenterica(Roxb.
IF 0.3 4区 生物学 Q4 PLANT SCIENCES Pub Date : 2023-12-31 DOI: 10.3329/bjb.v52i4.70609
C. Dash, Nowshin Anjum, Prasenjit Roy, Syeda Sharmeen Sultana
The karyomorphological features of Holarrhena antidysenterica (Roxb. ex Fleming) Wall. through the application of differential staining techniques (using orcein, CMA, and DAPI) were studied. After applying orcein staining, the interphase nuclei of this species exhibited a ‘simple chromocenter type’ with numerous small heterochromatin blocks and the prophase chromosomes were ‘interstitial type’. The karyotype analysis revealed that this species possesses diploid chromosome number of 2n = 22, with all metacentric chromosomes. The total length of the chromosome complement in the diploid set was 29.91 μm. A total of four CMA-positive bands were observed in this species whereas seven bands were found after DAPI-staining. Heteromorphicity was observed in the DAPI stained metaphase stage indicating the probable occurrences of chromosomal aberrations such as deletion and inversion. The karyotype profile of H. antidysenterica obtained from orcein and fluorescent banding was determined for the first time in Bangladesh. Bangladesh J. Bot. 52(4): 1085-1089, 2023 (December)
通过应用不同的染色技术(使用orcein、CMA和DAPI),研究了Holarrhena antidysenterica (Roxb. ex Fleming) Wall.的核形态学特征。在使用橙皮苷染色后,该物种的间期细胞核表现为 "简单染色中心型",其中有许多小的异染色质块,而前期染色体则为 "间隙型"。核型分析表明,该物种的二倍体染色体数为 2n = 22,全部为偏心染色体。二倍体染色体互补体的总长度为 29.91 μm。该物种共观察到四条 CMA 阳性条带,而 DAPI 染色后则发现七条条带。在 DAPI 染色的移行期观察到异形,表明可能存在染色体畸变,如缺失和倒位。在孟加拉国,首次确定了通过橙皮苷和荧光带染色获得的 H. antidysenterica 的核型特征。孟加拉国 J. Bot.52(4):1085-1089, 2023 (December)
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引用次数: 0
Evaluation of antibiotics and bioagents for the management of black rot disease of cabbage and their effect on extracellular polysaccharide secretion 评估抗生素和生物制剂对甘蓝黑腐病的防治作用及其对细胞外多糖分泌的影响
IF 0.3 4区 生物学 Q4 PLANT SCIENCES Pub Date : 2023-12-31 DOI: 10.3329/bjb.v52i4.70578
P. Sangwan, Rakesh Sangwan, Vinod Malik, Manjeet Singh
Black rot disease caused by bacterium Xanthomonas campestris pv. campestris has been identified as major cause for low cabbage yields. The present study was conducted to evaluate the efficacy of various antibiotics and bioagents for controlling black rot disease and also their effect on extracellular polysaccharide secretion (EPS) of Xanthomonas campestris pv. campestris. Under in vitro conditions, bacterial proliferation and EPS production were examined on yeast peptone agar medium containing sucrose or trehalose with or without antibiotics. Number of Xanthomonas campestris pv. campestris on yeast peptone agar medium with Validamycin-A was less as compared to control. Extracellular polysaccharide secretion (EPS) was also inhibited on yeast peptone agar medium with antibiotic Validamycin-A. In vivo, disease intensity was recorded minimum in seed treatment + root dipping + foliar spray with Validamycin-A @ 500 µg/ml followed by seed treatment with Validamycin-A @ 500 µg/ml in comparison to control. Similarly, 45.61% higher cabbage yield was recorded in seed treatment + root dipping + foliar spray with Validamycin-A @ 500 as compared to untreated control. However, the antibiotic Validamycin-A which was found effective can be recommended to manage the disease. Bangladesh J. Bot. 52(4): 965-970, 2023 (December)
野油菜黄单胞菌(Xanthomonas campestris pv. campestris)引起的黑腐病已被确定为白菜产量低的主要原因。本研究旨在评估各种抗生素和生物制剂对控制黑腐病的功效,以及它们对野菜黄单胞菌胞外多糖分泌(EPS)的影响。在体外条件下,在含有或不含抗生素的蔗糖或曲海糖的酵母蛋白胨琼脂培养基上检测细菌增殖和 EPS 产量。在含 Validamycin-A 的酵母胨琼脂培养基上,野油菜黄单胞菌 pv. campestris 的数量比对照组少。在含有抗生素 Validamycin-A 的酵母蛋白胨琼脂培养基上,胞外多糖分泌(EPS)也受到抑制。在体内,与对照相比,种子处理 + 根部浸泡 + 叶面喷洒 Validamycin-A @ 500 µg/ml 的病害强度最小,其次是种子处理 + Validamycin-A @ 500 µg/ml 的病害强度。同样,与未处理的对照相比,用 Validamycin-A @ 500 进行种子处理 + 根部浸种 + 叶面喷洒的白菜产量提高了 45.61%。不过,可以推荐使用有效的抗生素 Validamycin-A 来控制该病害。孟加拉国植物学杂志52(4):965-970,2023 年(12 月)
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引用次数: 0
Potential of compound bacterial agent in the biocontrol of muskmelon pathogens 复合菌剂在麝香瓜病原体生物防治中的潜力
IF 0.3 4区 生物学 Q4 PLANT SCIENCES Pub Date : 2023-12-31 DOI: 10.3329/bjb.v52i4.70603
R. Fu, Hong Zhang, Tieqi Xia, Xue Yang, Dingwang, Wu-ling Chen
The bacterial strain TG6 was screened from the soil of a muskmelon greenhouse, which could effectively inhibit the growth of Sphaerotheca fuliginea, Fusarium oxysporum, Phytophthora melonis and Pseudoperonospora cubensis of muskmelons. TG6 was identified as Bacillus subtilis through morphological, physiological, biochemical and molecular studies. Taking TG6 as the original strain, TG67 and TG69 were obtained by He-Ne laser mutagenesis. The microbial compound antagonist strain TG67 and TG69 was prepared in the ratio of 1:1 and applied to the biological control of greenhouse melon. Muskmelon field experiments showed that the bactericide could effectively control the occurrence of various diseases of muskmelon in greenhouse. Bangladesh J. Bot. 52(4): 1047-1053, 2023 (December)
从麝香瓜温室的土壤中筛选出细菌菌株 TG6,该菌株能有效抑制麝香瓜的褐斑病菌(Sphaerotheca fuliginea)、氧孢镰刀菌(Fusarium oxysporum)、瓜疫霉(Phytophthora melonis)和立方假包囊病菌(Pseudoperonospora cubensis)的生长。通过形态学、生理学、生物化学和分子研究,确定 TG6 为枯草芽孢杆菌。以 TG6 为原始菌株,通过氦氖激光诱变获得了 TG67 和 TG69。按照 1:1 的比例制备了微生物复合拮抗剂菌株 TG67 和 TG69,并将其应用于温室甜瓜的生物防治。西瓜田间试验表明,该杀菌剂可有效控制温室西瓜各种病害的发生。孟加拉国植物学报52(4):1047-1053, 2023 (December)
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引用次数: 0
Effects of seedling dipping and foliar application of nano dap on growth, yield and economics of fine rice 秧苗浸种和叶面喷施纳米 Dap 对水稻生长、产量和经济效益的影响
IF 0.3 4区 生物学 Q4 PLANT SCIENCES Pub Date : 2023-12-31 DOI: 10.3329/bjb.v52i4.70589
Meenakshi Attri, Neetu Sharma, Swati Mehta, Joysamuel Mecarty
A field experiment was conducted to assess the effect of seedling dipping and foliar application of phosphorus on growth, yield and economics of fine rice. The experimental results revealed that 75% recommended nitrogen, phosphorus and 100% recommended potassium + seedling dipping with nano di-ammonium phosphate @ 5 ml/litre + foliar spray with nano di-ammonium phosphate @ 4 ml/litre application recorded significantly higher growth, yield and yield attributes, which was statistically at par with, 50% recommended nitrogen, phosphorus +100% recommended potassium + seedling treatment with nano di-ammonium phosphate @ 5 ml/litre+ 2 foliar spray with nano di-ammonium phosphate each @ 4 ml/litre and @ 2 ml/litre and 100% recommended nitrogen, phosphorus and potassium. However, 50% recommended nitrogen, phosphorus +100% recommended potassium + seedling treatment with nano di-ammonium phosphate @ 5 ml/litre+ 2 foliar spray with nano nano di-ammonium phosphate each @ 4 ml/litre recorded significantly highest agro physiological efficiency. However, in terms of economics 75% recommended nitrogen, phosphorus and 100% recommended potassium + seedling dipping with nano di-ammonium phosphate @ 5 ml/litre + foliar spray with nano nano di-ammonium phosphate @ 4 ml/litre recorded highest net returns and benefit cost ratio. Bangladesh J. Bot. 52(4): 1025-1031, 2023 (December)
进行了一项田间试验,以评估秧苗浸种和叶面喷施磷肥对水稻生长、产量和经济效益的影响。实验结果表明,75% 的氮磷推荐量和 100% 的钾推荐量 + 5 毫升/升的纳米磷酸二铵浸种 + 4 毫升/升的纳米磷酸二铵叶面喷施能显著提高生长、产量和产量属性、在统计学上,这与 50%的推荐氮、磷 +100% 的推荐钾 + 5 毫升/升的纳米磷酸二铵苗期处理 + 4 毫升/升和 2 毫升/升的纳米磷酸二铵叶面喷施以及 100%的推荐氮、磷和钾相当。然而,50%的氮、磷推荐量+100%的钾推荐量+纳米磷酸二铵(5 毫升/升)苗期处理+纳米磷酸二铵(4 毫升/升)2 次叶面喷施的农业生理效率明显最高。然而,就经济效益而言,75% 的氮磷推荐量和 100% 的钾推荐量 + 5 毫升/升的纳米磷酸二铵浸种 + 4 毫升/升的纳米磷酸二铵叶面喷施,都能获得最高的净收益和效益成本比。孟加拉国植物学报52(4):1025-1031,2023 年(12 月)
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引用次数: 0
Molecular characterization and diversity analysis of promising tgms lines and high-yieding varieties In rice by using ssr markers 利用 ssr 标记对有潜力的 tgms 品系和高结实率水稻品种进行分子表征和多样性分析
IF 0.3 4区 生物学 Q4 PLANT SCIENCES Pub Date : 2023-12-31 DOI: 10.3329/bjb.v52i4.70610
V. Karpagam, R. Kalaiyarasi
The present investigation was intended to assess the extent of genetic diversity present among the selected genotypes of Oryza sativa (including TGMS lines) using SSR markers. The SSR markers used for the study gave average Polymorphism Information Content (PIC) value of 0.551. The primer RM 9 followed by RM 536 has given maximum PIC value and the primer RM 29 gave the minimum. It was inferred that the primers RM 9 and RM 536 had been the better choices in this study. The lines, TS09 24 and TS09 26 were in the same cluster as they derived from the same parents. The TGMS lines TS 29 and TNAU 27S are in separate cluster from other parents indicating that these lines are highly diversified and their crosses can give improved cultivars. Bangladesh J. Bot. 52(4): 1067-1070, 2023 (December)
本研究旨在利用 SSR 标记评估所选野生稻基因型(包括 TGMS 品系)的遗传多样性程度。研究中使用的 SSR 标记的平均多态性信息含量(PIC)值为 0.551。引物 RM 9 和 RM 536 的 PIC 值最大,引物 RM 29 的 PIC 值最小。由此推断,在本研究中,引物 RM 9 和 RM 536 是较好的选择。TS09 24 和 TS09 26 这两个品系属于同一群,因为它们来自同一个亲本。TGMS 品系 TS 29 和 TNAU 27S 与其他亲本分在不同的群中,这表明这些品系的多样性很高,它们的杂交可以培育出改良的栽培品种。孟加拉国植物学杂志52(4):1067-1070,2023 年(12 月)
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引用次数: 0
Genotype and environment interactions of yield contributing characters of field mustard (Brassica rapa L.) 大田芥菜(Brassica rapa L.)产量贡献特征的基因型与环境交互作用
IF 0.3 4区 生物学 Q4 PLANT SCIENCES Pub Date : 2023-12-31 DOI: 10.3329/bjb.v52i4.70604
Susmita Mondal, F. T. Johora, Goutom Roy, Jamilur Rahman
Genotype-environment interaction is the norm of reciprocal action that determines the relationship between gene and environmental factors. Gene and environment interaction takes place when different environments affect the genotypes and vice versa. The investigation portrays the interaction between genes and environments of yield contributing characteristics of eleven genotypes of field mustard. Three  seeding-time environments, viz., early, late, and very late of eleven mustard genotypes were laid out with three replications in a randomized complete block design. Among the three environments, which one was favorable and which was not was determined by their significant differences through a combined analysis of variance. The IPCA 1 (first interaction principal component axis) score of genotypes in the AMMI (additive main effects and multiplicative interaction analysis) of G×E interactions was estimated as an indication of the ability to respond to the environments and the performance with changing environments of the genotypes. Considering all the scores, Sonali Sharisha (SS-75), and BINA Sharisha-10 were found to be highly stable genotypes, while among the three environments, the environment-1 (early sowing) was found the best sowing time for raising and harvesting a good mustard crop. Bangladesh J. Bot. 52(4): 1055-1065, 2023 (December)
基因型与环境之间的相互作用是决定基因与环境因素之间关系的互作准则。当不同的环境影响基因型时,基因与环境就会发生相互作用,反之亦然。本研究描述了 11 个大田芥菜基因型的产量贡献特性的基因与环境之间的相互作用。11 个芥菜基因型的三种播种时间环境,即早播、晚播和极晚播,在随机完全区组设计中进行了三次重复。在这三种环境中,哪种环境有利,哪种环境不利,是通过综合方差分析确定的。在 G×E 交互作用的 AMMI(加法主效应和乘法交互作用分析)中,对基因型的 IPCA 1(第一交互作用主成分轴)得分进行了估算,以表明基因型对环境的反应能力以及在环境变化时的表现。考虑到所有得分,Sonali Sharisha(SS-75)和 BINA Sharisha-10 被认为是高度稳定的基因型,而在三种环境中,环境-1(早播)被认为是培育和收获良好芥菜作物的最佳播种时间。孟加拉国植物学杂志52(4):1055-1065,2023 年(12 月)
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引用次数: 0
Changes in internal structure and nutritional quality with different types of maize kernels during Grain filling period 谷粒灌浆期不同类型玉米籽粒内部结构和营养质量的变化
IF 0.3 4区 生物学 Q4 PLANT SCIENCES Pub Date : 2023-12-31 DOI: 10.3329/bjb.v52i4.70608
Qiang Guo, Juan Li, Jingjing Cui, Huadi She
To understand the changes in grain internal structure and nutrients during maize filling, Jingkenuo 2000 (waxy corn), Chaosweet 2000 (sweet corn), and Zhengdan 958 (common corn) were used as test materials. This study observed the internal structure of the corn grain and measured the change in nutritional quality during the filling period. Throughout the determination stage, the starch granules did not fill the whole endosperm cell in Chaosweet 2000, and the cell wall was clearly visible. By contrast, the starch granules filled the whole endosperm cell in Jingkenuo 2000 and Zhengdan 958. The protein content in Chaosweet 2000 was significantly (P<0.05) higher than those in Jingkenuo 2000 and Zhengdan 958. With the extension of post-pollination time, the content of soluble sugar decreased significantly (P<0.05) in Chaosweet 2000, Jingkenuo 2000, and Zhengdan 958. Bangladesh J. Bot. 52(4): 1077-1084, 2023 (December)
为了解玉米灌浆期籽粒内部结构和营养成分的变化,本研究以京垦 2000(蜡质玉米)、超甜 2000(甜玉米)和正丹 958(普通玉米)为试验材料。这项研究观察了玉米籽粒的内部结构,并测定了玉米灌浆期营养品质的变化。在整个测定阶段,超甜 2000 的淀粉颗粒没有充满整个胚乳细胞,细胞壁清晰可见。相比之下,井研糯 2000 和正丹 958 的淀粉颗粒充满了整个胚乳细胞。朝甜 2000 的蛋白质含量明显高于京粳 2000 和郑单 958(P<0.05)。随着授粉后时间的延长,朝甜 2000、京果 2000 和正丹 958 的可溶性糖含量明显下降(P<0.05)。孟加拉国植物学报52(4):1077-1084,2023 年(12 月)
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引用次数: 0
Effects Of Organic Substance And Plant Growth Regulators On Yield Attributes And Quality Of Guava Cv. Lucknow 49 有机物质和植物生长调节剂对番石榴产量属性和质量的影响勒克瑙 49
IF 0.3 4区 生物学 Q4 PLANT SCIENCES Pub Date : 2023-12-31 DOI: 10.3329/bjb.v52i4.70579
DM Dabhi, Mj Patel, SJ Macwan
In the present study effects of organic substance and plant growth regulators on yield attributes and quality of guava cv. Lucknow 49 were conducted during 2021-2022. Results indicated that foliar application of novel organic liquid fertilizer (40 ml/l) gave higher yield parameters viz. number of flowers per branch, fruit set, weight per fruit, fruit volume, fruit diameter, fruit length, pulp weight per fruit, number of fruits per plant, fruit yield (kg/plant) during both the years and in pooled data. In quality parameters, higher chlorophyll content and pulp: seed ratio were recorded with novel organic liquid fertilizer (40 ml/l) during both the years and in pooled data. However, organic substance and plant growth regulators treatments showed non-significant effect on total seed weight per fruit in both the years as well as in pooled data. Bangladesh J. Bot. 52(4): 971-978, 2023 (December)
本研究在 2021-2022 年期间进行,研究了有机物质和植物生长调节剂对番石榴品种 Lucknow 49 的产量属性和质量的影响。结果表明,叶面喷施新型有机液体肥料(40 毫升/升)可提高产量参数,即每枝花数、坐果率、单果重、果实体积、果实直径、果实长度、单果果肉重、单株果实数、果实产量(公斤/株)。在质量参数方面,使用新型有机液体肥料(40 毫升/升)的年份和汇总数据均显示叶绿素含量和果肉种子比更高。然而,有机物质和植物生长调节剂处理对两个年份和汇总数据中每颗果实的种子总重量均无显著影响。孟加拉国植物学杂志》(Bangladesh J. Bot.52(4):971-978,2023 年(12 月)
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引用次数: 0
Optimisation of seed germination and seedling emergence of sesbania cannabina (retz.) Poir. 优化 sesbania cannabina (retz.) Poir.
IF 0.3 4区 生物学 Q4 PLANT SCIENCES Pub Date : 2023-12-31 DOI: 10.3329/bjb.v52i4.70588
Abdul Kadir Ibne Kamal, Lesley C Batty, Rebecca Bartlett
This study aims to optimise the condition for seed germination and seedling emergence of Sesbania cannabina (Retz.) Poir. for a phytoremediation study. In this experiment, seed germination was carried out using two growth media: Murashige and Skoog basal medium (MS); and Whatman Grad 1 filter paper (FP), using the top of media or top of the paper method under three different photoperiods. Seeds were pre-treated for different lengths of time with various concentrations of hydrogen peroxide (H2O2) (v/v) multiple hot water treatments. Results showed that seeds pre-treated with H2O2 (6% v/v) for 5 minutes and primed with 65°C water for 5 minutes were considered as the ideal pre-treatment condition. Different photoperiods and media used in germination in this experiment do not significantly affect seed germination. The optimum condition for seed germination at 27.5 °C ± 2.5°C and relative humidity of ~ 75% for 5 days. Seed emergence in soil and compost was significantly affected by the burial depth and bulk density of the media, with the highest (98%±1) seed emergence observed at 1 cm depth for soil and compost, and decreasing with increased burial depth. This ideal condition will help in further studies related to plant growth and phytoremediation of S. cannabina. Bangladesh J. Bot. 52(4): 1015-1023, 2023 (December)
本研究旨在优化 Sesbania cannabina (Retz.) Poir.的种子发芽和出苗条件,以进行植物修复研究。本实验使用两种生长培养基进行种子萌发:在三种不同的光照周期下,使用两种生长培养基进行种子萌发,一种是 Murashige 和 Skoog 基础培养基(MS),另一种是 Whatman Grad 1 滤纸(FP),使用的方法是培养基顶部法或滤纸顶部法。用不同浓度的过氧化氢(H2O2)(v/v)对种子进行不同时间长度的多次热水预处理。结果表明,种子用 H2O2(6% v/v)预处理 5 分钟并用 65°C 水浸泡 5 分钟被认为是理想的预处理条件。本实验中不同的光周期和发芽培养基对种子发芽没有明显影响。种子萌发的最佳条件为 27.5 °C ± 2.5°C,相对湿度约为 75%,持续 5 天。种子在土壤和堆肥中的萌发明显受到培养基埋藏深度和容重的影响,土壤和堆肥在 1 厘米深时种子萌发率最高(98%±1),随着埋藏深度的增加,种子萌发率逐渐降低。这一理想条件将有助于进一步研究大麻属植物的生长和植物修复。孟加拉国植物学杂志52(4):1015-1023,2023 年(12 月)
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引用次数: 0
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Bangladesh Journal of Botany
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