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Effect of stingless bee (Hymenoptera: Apidae: Apinae: Meliponini) propolis on the proliferation of human stem cells 无刺蜂(膜翅目:蜂科:蜂科)蜂胶对人干细胞增殖的影响
Pub Date : 2022-12-31 DOI: 10.33307/entomon.v47i4.788
Drishya Prakashan, R. J. Nija, G. Krishnan, K. Soni, S. Alex, Smita Sudheer, S. Shanas
The effect of stingless bee propolis on the proliferation and differentiation of human stem cells is reported for the first time. Human pluripotent stem cells (hPSCs) treated with the propolis extracted from Lisotrigona sp, Tetragonula calophyllae and T. travancorica (Hymenoptera: Apidae: Apinae: Meliponini) displayed a remarkable difference in their morphology. Gene expression analysis revealed pluripotency markers OCT4 and NANOG to be down-regulated upon treatment with propolis, which confirmed early differentiation of hPSCs. Further investigation on the gene expression of early differentiation markers revealed that propolis supports mesendoderm differentiation, which is a novel finding. The propolis obtained from stingless bees Tetragonula spp. probably has more therapeutic value in terms of its effect on hPSCs viz., more tendency of the cells to differentiate into mesoderm and endoderm lineages, compared to the propolis obtained from Lisotrigona sp.
本文首次报道了无刺蜂胶对人干细胞增殖和分化的影响。人多能干细胞(hPSCs)经三种植物蜂胶处理后(膜翅目:蜂科:蜂科:蜂科)表现出明显的形态学差异。基因表达分析显示,多能性标志物OCT4和NANOG在蜂胶处理后下调,证实了hPSCs的早期分化。进一步对早期分化标志物基因表达的研究发现蜂胶支持中胚层分化,这是一个新发现。与Lisotrigona sp.相比,从无刺蜜蜂Tetragonula sp.获得的蜂胶在对人乳头状细胞的作用方面可能具有更大的治疗价值,即细胞更倾向于向中胚层和内胚层分化。
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引用次数: 0
Chemical characterization of n-alkane compounds in the leaves of Holoptelea integrifolia and its repellence against Japanese encephalitis vector 整叶全息蕨叶中正构烷烃化合物的化学特征及其对日本脑炎病媒的驱避作用
Pub Date : 2022-12-31 DOI: 10.33307/entomon.v47i4.805
S. Singha, G. Chandra
Epicuticular wax extract bearing n-alkane compounds were isolated from leaves of Holoptelea integrifolia and its chemical characterization was done by GC-MS analysis. Seven n-alkane compounds were isolated from epicuticular wax of H. integrifolia, which are Undecane [C11H24], Decane 5-methyl- [C11H24], Dodecane [C12H26], Undecane, 3,6-dimethyl- [C12H26], Hexadecane, 2,6,10,14-tetramethyl- [C20H42], Tridecane [C13H28], and Tetradecane [C14H30]. Different concentrations of crude extract as well as epicuticular wax extract bearing n-alkane each @ 2, 4 and 5 ppm cm-2 applied on human hand surface for repellence against Culex vishnui (vectors of JE) and at different time of exposure, gave a maximum protection of 73.33 per cent in the case of crude extract, and 94.33 per cent with epicuticular wax extract, both at 5 ppm cm-2, up to five hours of exposure.
从整叶全息草叶片中分离得到含正烷烃化合物的表皮蜡提取物,并采用气相色谱-质谱分析对其进行化学表征。从石蜡中分离得到7个正构烷烃化合物,分别为十一烷[C11H24]、癸烷5-甲基- [C11H24]、十二烷[C12H26]、十一烷3,6-二甲基- [C12H26]、十六烷2,6,10,14-四甲基- [C20H42]、十三烷[C13H28]和十四烷[C14H30]。将不同浓度的粗提取物和表皮蜡提取物分别含有2、4和5 ppm cm-2的正烷烃,并在不同的暴露时间涂抹在人的手表面,以驱避vishnui库蚊(乙脑载体),在5 ppm cm-2的情况下,粗提取物的最大保护率为73.33%,表皮蜡提取物的最大保护率为94.33%,暴露时间长达5小时。
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引用次数: 0
Additional record of the little known xylophagous endemic wood roach Salganea rehni Roth, 1979 (Blattodea, Blaberidae, Panesthiinae) from the Western Ghats, India with its DNA barcode 印度西高止山脉鲜为人知的地方性食木蟑螂Salganea rehni Roth, 1979(小蠊科,小蠊科,大蠊科)的附加记录及其DNA条形码
Pub Date : 2022-12-31 DOI: 10.33307/entomon.v47i4.799
A. Kalawate, A. Shabnam, K. Dinesh
The paper presents the record of the poorly known endemic species of wood roach from India after a gap of almost 40 years. In India, discernible work has been done on the DNA barcode of cockroaches including the genus Salganea. This work forms the first mitochondrial DNA barcode for the species Salganea rehni Roth, 1979.
本文介绍了一种鲜为人知的印度特有种木蟑螂的记录,经过近40年的差距。在印度,已经对蟑螂的DNA条形码进行了可识别的工作,其中包括Salganea属。这项工作形成了1979年Salganea rehni Roth物种的第一个线粒体DNA条形码。
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引用次数: 0
Checklist of spiders from Vallakadavu range of Western Ghats, Kerala, India 印度喀拉拉邦西高止山脉Vallakadavu地区蜘蛛清单
Pub Date : 2022-09-30 DOI: 10.33307/entomon.v47i3.774
Linta Joseph, K. Jose
In the preliminary study conducted to document the spider fauna in Vallakadavu range, Idukki district, Kerala, a total of 33 species of spiders belonging to 29 genera from 12 families were recorded. Among the 12 families recorded, Araneidae was the most predominant with orb web weavers. From the guild analysis, the recorded families were categorized into seven principle types of web patterns.The study extends the range of  Poecilotheriastriata Pocock, 1895 in the state to the east.
本文对喀拉拉邦伊杜基县Vallakadavu地区蜘蛛区系进行了初步研究,共记录到12科29属33种蜘蛛。在记录的12科中,蜘蛛科以网蛛为主。通过行会分析,将所记录的家族划分为7种主要的网络类型。该研究将1895年在该州的Poecilotheriastriata Pocock的范围扩展到东部。
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引用次数: 0
Field evaluation of management strategies against Lipaphis erysimi (Kaltenbach) (Homoptera: Aphididae) infesting Indian mustard in Haryana, India 印度哈里亚纳邦芥菜区褐蚜(同翅目:蚜虫科)田间防治策略评价
Pub Date : 2022-09-30 DOI: 10.33307/entomon.v47i3.760
H. Kumar, Sumer Singh, A. Yadav, Mahesh Kumar
Effectiveness of diverse eco-safe strategies against mustard aphid, Lipaphis erysimi (Kaltenbach) infesting Indian mustard was evaluated for two years with 11 treatments viz., Beauveria bassiana @ 108 CS ml-1, neem seed kernel extract (NSKE) @ 5 per cent, neem oil @ 5 per cent, B. bassiana @ 108 CS ml-1 after clipping of infested twigs (CIT), nimbecidine @ 0.03 per cent, NSKE @ 5 per cent after CIT, neem oil @ 5 per cent after CIT, nimbecidine @ 0.03 per cent  after CIT, clipping of infested twigs alone, dimethoate 30 EC @ 625 ml ha-1 and control. The pooled data revealed that dimethoate contributed maximum efficacy in reducing L. erysimi population over control (89.74 %), followed by B. bassiana after CIT (83.16 %) and nimbecidine @ 0.03 per cent after CIT (80.51%). Seed yield (1716 kg ha-1) was maximum in dimethoate , followed by treatments B. bassiana @ 108 CS ml-1 after CIT (1636.5 kg ha-1) and nimbecidine @ 0.03 per cent  after CIT (1608 kg ha-1), whereas minimum (1211 kg ha-1) in the control. The gross income (Rs 64350 ha-1) and net return (Rs 18017 ha-1) were highest in dimethoate, followed by B. bassiana @ 108 CS       ml-1 after CIT with gross income of Rs 61388 ha-1 and net return of Rs 13865 ha-1. The incremental cost-benefit ratio was also maximum in dimethoate (1: 19.58), followed by  B. bassiana, nimbecidine and NSKE treatments (1: 6.33 to 7.27). Results suggest that  B. bassiana @ 108 CS ml-1 after CIT and nimbecidine @ 0.03 per cent after CIT can be used as a non- chemical control option as a substitute to chemical control.
不同eco-safe策略的有效性对芥末蚜虫,Lipaphis erysimi (Kaltenbach)——印度芥菜评估与11治疗即。两年来,白僵菌@ 108 CS ml-1,印楝种子内核提取(NSKE) @ 5%,印楝油@ 5%,单独使用@ 108 CS ml-1出没的树枝(CIT)剪裁后,nimbecidine @ 0.03%, CIT NSKE @ 5%后,印楝油@ CIT 5%之后,CIT nimbecidine @ 0.03%后,单独修剪受感染的树枝,乐果30 EC @ 625 ml ha-1和对照。综合数据显示,乐果对水蛭种群的减少效果最大(89.74%),其次是球孢白杆菌(83.16%)和尼贝西定(0.03%)(80.51%)。种子产量最高的是乐果,为1716 kg ha-1,其次是108 CS ml-1处理(1636.5 kg ha-1)和0.03%尼贝西定处理(1608 kg ha-1),而对照最低(1211 kg ha-1)。乐果处理的毛收入(64350 ha-1卢比)和净收益(18017 ha-1卢比)最高,CIT处理后的球孢白球菌次之,毛收入61388 ha-1卢比,净收益13865 ha-1卢比。增加成本效益比以乐果为主(1:19 .58),其次为球孢白僵菌、尼贝西定和NSKE(1:6 .33 ~ 7.27)。结果表明,CIT处理后的球孢白僵菌@ 108 CS ml-1和CIT处理后的尼贝西定@ 0.03%可作为替代化学防治的非化学防治选择。
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引用次数: 0
Morphological investigations on the wing scales of four species of common Indian butterflies 四种常见印度蝴蝶翅鳞的形态学研究
Pub Date : 2022-09-30 DOI: 10.33307/entomon.v47i3.759
K.P. Sijina, D. A. Evans
Wing scales of butterflies exhibit extreme diversity in shape, size, colour, and number of spines. They are sub microscopic with a length of 300 to 600µm and a breadth of 150 to 400µm. A typical scale possessed a flat body with basal pedicel and apical crown which is provided with a varying number of pointed edges called spines. Investigations were carried outon the morphology of wing scales in four species of common butterflies viz., Pachilopta hector (Linnaeus 1758), Troides minos (Cramer 1779), Jamides celeno (Cramer1775) and  Eurema andersonii (Linnaeus 1758). Wings of  P. hector possessed nine types of scales, the crown of all are with pointed spines of varying numbers, ranging from one to five. The southern birdwing T. minos, possessed nine types of scales. A major portion of wings with black colour is due to black coloured scales but white bands of the forewings are due to transparent and colourless scales. The prominent yellow colour on the hind wings of this butterfly is due to the presence of a single type of scale with a round crown that is devoid of spines and is fully packed with yellow pigment. Jamides celeno possessed twenty different types of scales, most of them are devoid of spines and the ridges within the scales are not clear. Scales on the upper surface of the wing with ashy blue colour and scales of white bands on the lower surface of the wings are identical and are transparent and colourless. The common grass yellow E. andersonii possessed twentyfive different types of scales, of which thirteen are on the black margins of wings and twelve are in the yellow portions of wings. Almost half of the total number of scales in the yellow portions of the wing is transparent and colourless and in coloured scales distribution of pigment is not uniform.This is the first report on the different types of wings scales in the selected butterflies.
蝴蝶的翅鳞在形状、大小、颜色和刺的数量上表现出极大的多样性。它们是亚微观的,长度为300至600微米,宽度为150至400微米。典型的鳞片具有扁平的体,基部有花梗,顶端有不同数量的尖边,称为棘。对4种常见蝴蝶(Pachilopta hector (Linnaeus 1758)、Troides minos (Cramer 1779)、Jamides celeno (Cramer1775)和Eurema andersonii (Linnaeus 1758))翅鳞形态进行了研究。翅膀上有九种鳞片,所有的冠上都有不同数量的尖刺,从1到5不等。南翅鸟T. minos有九种鳞片。黑色翅膀的主要部分是由于黑色的鳞片,但前翼的白色带是由于透明和无色的鳞片。这种蝴蝶后翅上突出的黄色是由于一种单一类型的鳞片的存在,这种鳞片有一个圆形的冠,没有刺,充满了黄色颜料。贾米德塞利诺有20种不同类型的鳞片,大多数鳞片没有刺,鳞片内的脊不清楚。翅膀上表面的鳞片呈灰蓝色,翅膀下表面的鳞片呈白色带,两者相同,透明无色。常见的草黄色安德氏线虫有25种不同类型的鳞片,其中13种在翅膀的黑色边缘,12种在翅膀的黄色部分。在翅膀的黄色部分,几乎一半的鳞片是透明和无色的,在彩色鳞片中,色素的分布是不均匀的。本文首次报道了所选蝴蝶翅鳞的不同类型。
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引用次数: 0
New distributional record of Argyrodes bonadea Karsch, 1881 and Argyrodes nephilae Taczanowski, 1873 from Kerala, India 印度喀拉拉邦bonadea Karsch(1881)和nephilae Taczanowski(1873)分布新记录
Pub Date : 2022-09-30 DOI: 10.33307/entomon.v47i3.766
R. Sekhar, K. Jose
Argyrodes Simon, 1864 is one of the rich genera of Theridiidae. For the first time A.bonadea Karsch, 1881 and A. nephilae Taczanowski, 1873 were reported from Kerala. Digital photographs are used to redescribe the species.
银蛛属(Argyrodes Simon, 1864)是银蛛科丰富的属之一。首次在喀拉拉邦报道了A.bonadea Karsch(1881)和a.n nephilae Taczanowski(1873)。数码照片被用来重新描述这个物种。
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引用次数: 0
A new species of Protosticta Selys, 1885 (Odonata: Zygoptera: Platystictidae) from the Brahmagiri Hills, Kerala, India 印度喀拉拉邦Brahmagiri山原蜱属一新种(齿翅目:棘翅目:棘蜱科)
Pub Date : 2022-09-30 DOI: 10.33307/entomon.v47i3.761
Vibhu Vijayakumaran, V. P. Nair, A. Samuel, M. Palot, K. Sadasivan
A new species of  Protosticta Selys, 1885 is described from Brahmagiri hills of Coorg landscape of the Western Ghats in Peninsular India. The new species Protosticta francyisp. nov., is a congener of  P. antelopoides Fraser, 1931 and  P. ponmudiensis Kiran, Kalesh & Kunte, 2015, occupying a similar microhabitat, but distributed north of the major biogeographical divide, the Palghat Ga p. The new taxon is distinguished from all other Protosticta of  Western Ghats by the presence of long prothoracic spines in the males, the structure of the tip of the male cerci and genital ligula. A revised key to the species of  Protosticta of  Western Ghats is provided based on mature males.
印度半岛西高止山脉的Brahmagiri山描述了一种新的原生植物Selys, 1885。新种原蝇属。nov.,是p. antelopoides Fraser(1931)和p. ponmudiensis Kiran (Kalesh & Kunte)(2015)的同族,占据相似的微栖息地,但分布在主要生物地理分界线Palghat Ga p的北部。该新分类群与西高止山脉所有其他原生动物的区别在于,雄性中存在长长的前胸棘,雄性尾毛尖端的结构和生殖器舌。本文以成熟雄虫为基础,对西高止山脉原生虫种进行了分类。
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引用次数: 5
Spider fauna (Araneae: Arachnida) in different localities of Kannur District, Kerala, India 印度喀拉拉邦坎努尔地区不同地区的蜘蛛区系(蜘蛛目:蛛形纲)
Pub Date : 2022-09-30 DOI: 10.33307/entomon.v47i3.770
S. Swapna, K. Babitha
A survey conducted to reveal on diversity in different areas in Kannur District, Kerala, indicated a total of 31 species belonging to 15 families. The families Salticidae and Araneidae showed maximum species diversity. The study revealed that the selected study areas have favorable microhabitats for the spider fauna.
一项旨在揭示喀拉拉邦坎努尔地区不同地区多样性的调查显示,共有15科31种。水蛛科和蜘蛛科的物种多样性最大。研究表明,所选研究区具有良好的蜘蛛区系微生境。
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引用次数: 0
Metabolites in galls induced on the leaves of Trewian udiflora (L.) (Euphorbiaceae) by Trioza fletcheri Crawford (Hemiptera: Triozidae) Trioza fletcheri Crawford(半翅目:Trioza Triozidae)诱导大戟科Trewian udiflora (L.)叶片胆囊代谢产物的研究
Pub Date : 2022-09-30 DOI: 10.33307/entomon.v47i3.757
O. Datta, S. Tomar
Trioza fletcheri Crawford is a sap-sucking psyllid that induces galls on Trewia nudiflora leaves. Early stages of T. fletcheri feed on parenchyma, whereas late-stages and adults feed on phloem, causing galls which arise in an isolated, agglomerated mass and rosette form only on the abaxial surface of T. nudiflora leaves. The feeding action of immature stages induces changes in metabolites of host tissue and creates a nutrition sink for feeding. The biochemical study revealed that galled tissues had higher levels of metabolites (total soluble sugars, reducing sugars, total protein and free amino acids) than ungalled tissues, with average values measuring 3.4±0.09, 1.4±0.1, 0.63±0.03, 1.9±0.23, 3.0±0.72mg/gdw in ungalled leaves; 4.3±0.02, 2.9±0.3, 1.9±0.47, 3.7±0.36, 4.7±0.53 mg/g dw in young galls; 3.8±0.50, 3.7±0.3, 1.03±0.04, 2.9±0.35, 5.4±0.31 mg/g dw in mature galls; and 2.7±0.23, 2.4±0.3, 0.83±0.03, 2.6±0.34 and, 4.3±0.22mg/g dw in old galls, respectively. Enhanced activities of IAA-oxidase, á-amylase, peroxidase, and invertase were observed in galled infested leaves than in ungalled leaves, and their values were measured to be 2.45±0.53, 2.4±0.3, 0.9±0.2, and 3.7±0.5 in ungalled leaves, 2.92±0.32, 3.2±0.2, 1.9±0.5 and 4.5±0.3in young galls, 3.7±0.43, 3.6±0.4, 1.4±0.4, 4.3±0.2 in mature galls, and 2.51±0.03, 2.9±0.4, 1.4±0.4, 3.8±0.1 in old galls respectively.
三叶木虱是一种吸液木虱,能在杜鹃的叶子上产生瘿。早期的fletcheri以薄壁组织为食,而后期和成虫以韧皮部为食,形成孤立的、聚集的块状瘿,只在nudiflora叶片背面形成玫瑰花结。未成熟阶段的取食行为引起宿主组织代谢物的变化,并为取食创造营养汇。生化研究表明,剥蚀叶片代谢产物(总可溶性糖、还原糖、总蛋白和游离氨基酸)的平均值分别为3.4±0.09、1.4±0.1、0.63±0.03、1.9±0.23和3.0±0.72mg/gdw,高于未剥蚀叶片;4.3±0.02、2.9±0.3、1.9±0.47、3.7±0.36、4.7±0.53毫克/克dw年轻擦伤;成熟胆囊3.8±0.50、3.7±0.3、1.03±0.04、2.9±0.35、5.4±0.31 mg/g dw;老年性胆碱含量分别为2.7±0.23、2.4±0.3、0.83±0.03、2.6±0.34和4.3±0.22mg/g dw。剥蚀叶片的iaa -氧化酶、á-amylase、过氧化物酶和转化酶活性分别为2.45±0.53、2.4±0.3、0.9±0.2和3.7±0.5,幼龄叶片为2.92±0.32、3.2±0.2、1.9±0.5和4.5±0.3,成熟叶片为3.7±0.43、3.6±0.4、1.4±0.4、4.3±0.2,老龄叶片为2.51±0.03、2.9±0.4、1.4±0.4、3.8±0.1。
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引用次数: 0
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