Bioactive Materials最新文献_第6页

Near-infrared light-driven photocatalytic reactive oxygen species-generating antibacterial and self-shrinking hybrid hydrogels for combating drug-resistant bacterial biofilm infection and accelerating wound healing 近红外光驱动的光催化活性氧物种产生抗菌和自收缩混合水凝胶，用于对抗耐药细菌生物膜感染和加速伤口愈合

IF 18 1区医学 Q1 ENGINEERING, BIOMEDICAL

Bioactive Materials

Pub Date : 2026-01-02 DOI: 10.1016/j.bioactmat.2025.12.049

Dong Mo , Meng Pan , Yujia Wei , Yun Yang , Wen Chen , Qingya Liu , Xicheng Li , Jianan Li , Tianying Luo , Fan Yang , Hanzi Deng , Zhenpeng Zhang , Zhaolin Xiao , Kang Li , Zhiyong Qian

The development of wound dressings with tunable antibacterial activity and controllable self-shrinkage remains an intractable medical task for treating drug-resistant bacterial biofilm-infected wounds. Herein, we prepared a near-infrared-light (NIR)-controlled hydrogel (Co-BOS@C/F Gel) using an oxygen vacancy (OV)-rich cobalt (Co)-doped bismuth oxysulfide (Co-Bi₂O_2-XS, Co-BOS) photocatalyst as the guest molecule and a hydrazide-modified chondroitin sulfate/aldehyde-terminated Pluronic F127 hydrogel (C/F Gel) as the host carrier. First, the two-dimensional Co-BOS photocatalysts were synthesized via a one-step liquid-phase ion-exchange method. Co doping decreased the band gap, increased the number of OVs, and promoted charge transfer. Co-BOS also exhibited excellent photothermal performance (η = 34.09 %) and strong photocatalytic reactive oxygen species (ROS)-generating ability. The Co-BOS@C/F Gel formed via a dynamic Schiff reaction showed good temperature sensitivity, adhesion, hemostasis, and electrical conductivity and unique NIR-light-driven self-shrinkage. It exhibited broad-spectrum antibacterial activity against various bacteria and eliminated 95 % of the methicillin-resistant Staphylococcus aureus (MRSA) biofilm using photothermal therapy/antibacterial photocatalytic therapy. Integrated transcriptomic analyses revealed that the Gel operates via multiple antibacterial mechanisms, including the regulation of oxidative stress pathways and metabolic networks. Furthermore, this Gel significantly promoted cell proliferation and migration. In a mouse model of MRSA biofilm-infected wounds, the Co-BOS@C/F Gel under NIR light adaptively covered irregular wounds, eliminated MRSA biofilms, and accelerated wound closure. Without NIR light, the gel effectively promoted collagen deposition and angiogenesis. This study provides an innovative “kill four birds with one stone” strategy to treat drug-resistant bacteria-infected wounds and promotes the application of hybrid hydrogels in the biomedical field.

开发具有可调节抗菌活性和可控制自收缩的伤口敷料仍然是治疗耐药细菌生物膜感染伤口的一个棘手的医学课题。本文以富氧空位（OV）掺杂钴（Co）的硫化铋（Co- bi2o2 - xs, Co- bos）光催化剂为客体分子，以肼修饰硫酸软骨素/端醛的Pluronic F127水凝胶（C/F Gel）为载体，制备了近红外光（NIR）控制的水凝胶（Co-BOS@C/F Gel）。首先，采用一步液相离子交换法合成了二维Co-BOS光催化剂。Co掺杂减小了带隙，增加了OVs数量，促进了电荷转移。Co-BOS还表现出优异的光热性能（η = 34.09%）和较强的光催化活性氧（ROS）生成能力。通过动态希夫反应形成的Co-BOS@C/F凝胶具有良好的温度敏感性、粘附性、止血性和导电性，并具有独特的nir光驱动自收缩特性。它对多种细菌具有广谱抗菌活性，采用光热疗法/抗菌光催化疗法可消除95%的耐甲氧西林金黄色葡萄球菌（MRSA）生物膜。综合转录组学分析显示，凝胶通过多种抗菌机制起作用，包括氧化应激途径和代谢网络的调节。此外，该凝胶还能显著促进细胞的增殖和迁移。在MRSA生物膜感染的小鼠伤口模型中，Co-BOS@C/F凝胶在近红外光下适应性覆盖不规则伤口，消除MRSA生物膜，加速伤口愈合。在没有近红外光的情况下，凝胶有效地促进胶原沉积和血管生成。本研究为治疗耐药细菌感染伤口提供了一种“一石一鸟”的创新策略，促进了混合水凝胶在生物医学领域的应用。

{"title":"Near-infrared light-driven photocatalytic reactive oxygen species-generating antibacterial and self-shrinking hybrid hydrogels for combating drug-resistant bacterial biofilm infection and accelerating wound healing","authors":"Dong Mo , Meng Pan , Yujia Wei , Yun Yang , Wen Chen , Qingya Liu , Xicheng Li , Jianan Li , Tianying Luo , Fan Yang , Hanzi Deng , Zhenpeng Zhang , Zhaolin Xiao , Kang Li , Zhiyong Qian","doi":"10.1016/j.bioactmat.2025.12.049","DOIUrl":"10.1016/j.bioactmat.2025.12.049","url":null,"abstract":"<div><div>The development of wound dressings with tunable antibacterial activity and controllable self-shrinkage remains an intractable medical task for treating drug-resistant bacterial biofilm-infected wounds. Herein, we prepared a near-infrared-light (NIR)-controlled hydrogel (Co-BOS@C/F Gel) using an oxygen vacancy (OV)-rich cobalt (Co)-doped bismuth oxysulfide (Co-Bi<sub>2</sub>O<sub>2-X</sub>S, Co-BOS) photocatalyst as the guest molecule and a hydrazide-modified chondroitin sulfate/aldehyde-terminated Pluronic F127 hydrogel (C/F Gel) as the host carrier. First, the two-dimensional Co-BOS photocatalysts were synthesized <em>via</em> a one-step liquid-phase ion-exchange method. Co doping decreased the band gap, increased the number of OVs, and promoted charge transfer. Co-BOS also exhibited excellent photothermal performance (<em>η</em> = 34.09 %) and strong photocatalytic reactive oxygen species (ROS)-generating ability. The Co-BOS@C/F Gel formed via a dynamic Schiff reaction showed good temperature sensitivity, adhesion, hemostasis, and electrical conductivity and unique NIR-light-driven self-shrinkage. It exhibited broad-spectrum antibacterial activity against various bacteria and eliminated 95 % of the methicillin-resistant <em>Staphylococcus aureus</em> (MRSA) biofilm using photothermal therapy/antibacterial photocatalytic therapy. Integrated transcriptomic analyses revealed that the Gel operates <em>via</em> multiple antibacterial mechanisms, including the regulation of oxidative stress pathways and metabolic networks. Furthermore, this Gel significantly promoted cell proliferation and migration. In a mouse model of MRSA biofilm-infected wounds, the Co-BOS@C/F Gel under NIR light adaptively covered irregular wounds, eliminated MRSA biofilms, and accelerated wound closure. Without NIR light, the gel effectively promoted collagen deposition and angiogenesis. This study provides an innovative “kill four birds with one stone” strategy to treat drug-resistant bacteria-infected wounds and promotes the application of hybrid hydrogels in the biomedical field.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"59 ","pages":"Pages 396-420"},"PeriodicalIF":18.0,"publicationDate":"2026-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145881685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrical gastrodin-polyurethane spiral conduits with micro/nano-structure for accelerating peripheral nerve regeneration 加速周围神经再生的微纳米结构天麻素-聚氨酯电螺旋导管

IF 18 1区医学 Q1 ENGINEERING, BIOMEDICAL

Bioactive Materials

Pub Date : 2025-12-30 DOI: 10.1016/j.bioactmat.2025.12.035

Xiaoqian Lan , Guangli Feng , Qing Li , Shiyi Qin , Yingrui Hu , Shilin Pan , Jianlin Jiao , Di Lu , Lianmei Zhong

Peripheral nerve injuries tend to cause the proximal nerve unable to contact the corresponding target organ, resulting in sensory and motor dysfunction. The simple filling materials within nerve conduits are often inadequate for axonal ingrowth and directional regeneration. In this study, to enhance the guidance effect and achieve physiologically adaptive function, a 3D nanofibrous polyurethane (PU) scaffold with oriented microchannels was engineered using electrospinning and manual curling techniques. The electrospun fibrous membranes can be manually curled up into tubular structures with spiral and longitudinal multi-channels. The immunoregulatory and conductive properties were developed by being grafted gastrodin and aniline trimer (AT, 2.6 % and 5 %). Gastrodin stimulated proliferation of neural cells and expression of neuroblast-related genes. Electroactive AT produced an electrical signal in combination with electrical stimulation (ES) to accelerate the elongation and growth of Schwann cells (SCs) and neurite outgrowth of PC12 cells. The in vivo experiments revealed that the releasing gastrodin and electrical signals created prohealing microenvironment for alleviating inflammation and promoting vascularization. Of note, the topological structure provided well-organized internal support for the cells to spread, as well as the migration of SCs and the directional elongation of regenerating axons. The adaptive electroactivity of gastrodin-PU-AT5 % further ensured nerve signal transmission, ultimately promoted remyelination through upregulation of Rap1 and mTOR signaling pathways; thereby enhancing functional and structural regeneration. This scaffold design strategy will push forward the application of nerve conduits in long-distance peripheral nerve injury.

周围神经损伤往往使近端神经不能接触相应的靶器官，从而产生感觉和运动功能障碍。神经导管内的简单填充材料往往不能满足轴突向内生长和定向再生。本研究采用静电纺丝和人工卷曲技术，设计了一种具有定向微通道的三维纳米纤维聚氨酯（PU）支架，以增强其引导效果并实现生理自适应功能。电纺丝纤维膜可以手工卷曲成具有螺旋和纵向多通道的管状结构。将天麻素和苯胺三聚体（AT， 2.6%和5%）接植，获得了免疫调节和导电性能。天麻素刺激神经细胞增殖和神经母细胞相关基因的表达。电活性AT结合电刺激（ES）产生电信号，加速雪旺细胞（SCs）的伸长和生长以及PC12细胞的神经突生长。体内实验表明，释放天麻素和电信号可创造促愈合微环境，减轻炎症，促进血管化。值得注意的是，这种拓扑结构为细胞的扩散、SCs的迁移和再生轴突的定向伸长提供了组织良好的内部支持。天麻素- pu - at5%的适应性电活性进一步保证了神经信号的传递，最终通过上调Rap1和mTOR信号通路促进髓鞘再生；从而增强功能和结构的再生。这种支架设计策略将推动神经导管在远距离周围神经损伤中的应用。

{"title":"Electrical gastrodin-polyurethane spiral conduits with micro/nano-structure for accelerating peripheral nerve regeneration","authors":"Xiaoqian Lan , Guangli Feng , Qing Li , Shiyi Qin , Yingrui Hu , Shilin Pan , Jianlin Jiao , Di Lu , Lianmei Zhong","doi":"10.1016/j.bioactmat.2025.12.035","DOIUrl":"10.1016/j.bioactmat.2025.12.035","url":null,"abstract":"<div><div>Peripheral nerve injuries tend to cause the proximal nerve unable to contact the corresponding target organ, resulting in sensory and motor dysfunction. The simple filling materials within nerve conduits are often inadequate for axonal ingrowth and directional regeneration. In this study, to enhance the guidance effect and achieve physiologically adaptive function, a 3D nanofibrous polyurethane (PU) scaffold with oriented microchannels was engineered using electrospinning and manual curling techniques. The electrospun fibrous membranes can be manually curled up into tubular structures with spiral and longitudinal multi-channels. The immunoregulatory and conductive properties were developed by being grafted gastrodin and aniline trimer (AT, 2.6 % and 5 %). Gastrodin stimulated proliferation of neural cells and expression of neuroblast-related genes. Electroactive AT produced an electrical signal in combination with electrical stimulation (ES) to accelerate the elongation and growth of Schwann cells (SCs) and neurite outgrowth of PC12 cells. The <em>in vivo</em> experiments revealed that the releasing gastrodin and electrical signals created prohealing microenvironment for alleviating inflammation and promoting vascularization. Of note, the topological structure provided well-organized internal support for the cells to spread, as well as the migration of SCs and the directional elongation of regenerating axons. The adaptive electroactivity of gastrodin-PU-AT5 % further ensured nerve signal transmission, ultimately promoted remyelination through upregulation of Rap1 and mTOR signaling pathways; thereby enhancing functional and structural regeneration. This scaffold design strategy will push forward the application of nerve conduits in long-distance peripheral nerve injury.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"59 ","pages":"Pages 317-336"},"PeriodicalIF":18.0,"publicationDate":"2025-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145881689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorous oligoarginines as supra-enhancers for intracellular and transdermal peptide delivery 含氟低精氨酸作为细胞内和透皮肽传递的超级增强剂

IF 18 1区医学 Q1 ENGINEERING, BIOMEDICAL

Bioactive Materials

Pub Date : 2025-12-30 DOI: 10.1016/j.bioactmat.2025.12.027

Guangyu Rong , Qianqian Fan , Kunyu Chen , Yiyun Cheng , Jingjing Hu

Peptides are increasingly recognized as important therapeutics due to their specificity and potency. However, their clinical application is often hindered by numerous barriers, including low bioavailability, poor cell membrane and tissue penetration. To address these limitations, we developed a library of fluorous penetrating peptides and identified fluorinated hexa-arginine (FR6) as a super-enhancer for cell and tissue penetration. FR6 markedly enhances the intracellular delivery of a variety of peptides by improving membrane permeability, facilitating their uptake across diverse cell types. It greatly improves the penetration of bioactive peptides in 3D tumor spheroids, allowing for improved therapeutic efficacy. More importantly, FR6 demonstrates remarkable potential for transdermal delivery of peptide drugs, effectively enhancing the penetration of peptides like acetyl hexapeptide-8 for treating UVB-induced skin photoaging. Our findings underscore the use of FR6 as a super-enhancer in overcoming multiple physiological barriers during cell, tissue and transdermal delivery.

多肽由于其特异性和效力越来越被认为是重要的治疗药物。然而，它们的临床应用常常受到许多障碍的阻碍，包括生物利用度低、细胞膜和组织穿透性差。为了解决这些限制，我们开发了一个含氟穿透肽库，并确定了氟化六精氨酸（FR6）作为细胞和组织穿透的超级增强剂。FR6通过改善细胞膜通透性，促进多种多肽在不同细胞类型中的摄取，从而显著增强多种多肽的细胞内递送。它大大提高了生物活性肽在三维肿瘤球体中的渗透，从而提高了治疗效果。更重要的是，FR6在多肽药物的透皮递送方面表现出了显著的潜力，可以有效地增强乙酰六肽-8等肽的渗透，从而治疗uvb诱导的皮肤光老化。我们的研究结果强调了FR6作为一种超级增强剂在克服细胞、组织和透皮递送过程中的多重生理障碍中的作用。

{"title":"Fluorous oligoarginines as supra-enhancers for intracellular and transdermal peptide delivery","authors":"Guangyu Rong , Qianqian Fan , Kunyu Chen , Yiyun Cheng , Jingjing Hu","doi":"10.1016/j.bioactmat.2025.12.027","DOIUrl":"10.1016/j.bioactmat.2025.12.027","url":null,"abstract":"<div><div>Peptides are increasingly recognized as important therapeutics due to their specificity and potency. However, their clinical application is often hindered by numerous barriers, including low bioavailability, poor cell membrane and tissue penetration. To address these limitations, we developed a library of fluorous penetrating peptides and identified fluorinated hexa-arginine (FR6) as a super-enhancer for cell and tissue penetration. FR6 markedly enhances the intracellular delivery of a variety of peptides by improving membrane permeability, facilitating their uptake across diverse cell types. It greatly improves the penetration of bioactive peptides in 3D tumor spheroids, allowing for improved therapeutic efficacy. More importantly, FR6 demonstrates remarkable potential for transdermal delivery of peptide drugs, effectively enhancing the penetration of peptides like acetyl hexapeptide-8 for treating UVB-induced skin photoaging. Our findings underscore the use of FR6 as a super-enhancer in overcoming multiple physiological barriers during cell, tissue and transdermal delivery.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"59 ","pages":"Pages 305-316"},"PeriodicalIF":18.0,"publicationDate":"2025-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145881690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A large puncture closer of aortic wall by multi-memory actions with thrombo-hemodynamic control 通过多记忆作用对主动脉壁进行大穿刺，并控制血栓血流动力学

IF 18 1区医学 Q1 ENGINEERING, BIOMEDICAL

Bioactive Materials

Pub Date : 2025-12-30 DOI: 10.1016/j.bioactmat.2025.12.042

Sungwoo Cho , Hyun-Su Ha , Sangmin Lee , Hyunjae Kim , Seok Joon Lee , Jueun Kim , Yerin Lee , Kang Suk Lee , Hyun-Chel Joo , Hak-Joon Sung

The vascular wall regulates the pattern and pressure of blood flow. In cardiovascular interventions, catheters are deployed by puncturing the vessel wall, without exception. Despite continuous progress, the outcomes remain highly operator-dependent, and large punctures with high-pressure bleeding continue to pose clinical challenges. As a translatable solution, this study introduces a shape memory vascular wall plug (VWP) that automates both the Body and Wing functions within a single component, supported by a Ring assembly to maximize pressure resistance. The VWP is deployed into a 6-mm puncture in a porcine thoracic aorta under peak blood pressure, and shape recovery is triggered by a 45°C saline flush to enable automated activation. Upon recovery, Body expansion combined with Ring compression tightly seals the puncture tract. The curved Wing induces hemostatic sealing and then flattens to maintain healthy blood flow and physiologic pressures. The VWP achieves suturing-level performance in aortic puncture closure, demonstrating effective hemostasis, patency, and endothelialization. The flow-blockage ratio required to balance hemostasis with hemodynamics is computationally modeled and validated using whole-blood microfluidics. Pressure resistance is maximized by tuning Ring strain through polymer blending, indicating multi-level strategies in polymer, device design, and memory function to advance the vascular closure technology.

血管壁调节血液流动的模式和压力。在心血管干预中，导管无一例外地通过穿刺血管壁来部署。尽管取得了持续的进展，但结果仍然高度依赖于操作人员，高压出血的大穿刺继续构成临床挑战。作为一种可翻译的解决方案，该研究引入了一种形状记忆血管壁塞（VWP），该塞可以在单个组件内自动完成主体和翼的功能，并由环形组件支撑，以最大限度地提高耐压能力。在血压达到峰值的情况下，将VWP放入猪胸主动脉的6毫米穿刺处，并通过45°C盐水冲洗触发形状恢复，从而实现自动激活。恢复后，体膨胀结合环压缩紧密密封穿刺道。弯曲的翼诱导止血密封，然后变平以保持健康的血液流动和生理压力。VWP在主动脉穿刺闭合中达到缝合水平，显示出有效的止血、通畅和内皮化。血流堵塞比需要平衡止血与血流动力学计算建模和验证使用全血微流体。通过聚合物共混调节环应变，最大限度地提高抗压能力，这表明在聚合物、器件设计和记忆功能方面采取了多层次的策略来推进血管闭合技术。

{"title":"A large puncture closer of aortic wall by multi-memory actions with thrombo-hemodynamic control","authors":"Sungwoo Cho , Hyun-Su Ha , Sangmin Lee , Hyunjae Kim , Seok Joon Lee , Jueun Kim , Yerin Lee , Kang Suk Lee , Hyun-Chel Joo , Hak-Joon Sung","doi":"10.1016/j.bioactmat.2025.12.042","DOIUrl":"10.1016/j.bioactmat.2025.12.042","url":null,"abstract":"<div><div>The vascular wall regulates the pattern and pressure of blood flow. In cardiovascular interventions, catheters are deployed by puncturing the vessel wall, without exception. Despite continuous progress, the outcomes remain highly operator-dependent, and large punctures with high-pressure bleeding continue to pose clinical challenges. As a translatable solution, this study introduces a shape memory vascular wall plug (VWP) that automates both the Body and Wing functions within a single component, supported by a Ring assembly to maximize pressure resistance. The VWP is deployed into a 6-mm puncture in a porcine thoracic aorta under peak blood pressure, and shape recovery is triggered by a 45°C saline flush to enable automated activation. Upon recovery, Body expansion combined with Ring compression tightly seals the puncture tract. The curved Wing induces hemostatic sealing and then flattens to maintain healthy blood flow and physiologic pressures. The VWP achieves suturing-level performance in aortic puncture closure, demonstrating effective hemostasis, patency, and endothelialization. The flow-blockage ratio required to balance hemostasis with hemodynamics is computationally modeled and validated using whole-blood microfluidics. Pressure resistance is maximized by tuning Ring strain through polymer blending, indicating multi-level strategies in polymer, device design, and memory function to advance the vascular closure technology.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"59 ","pages":"Pages 288-304"},"PeriodicalIF":18.0,"publicationDate":"2025-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145881750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial synthesis of personalized biomimetic biological cornea 个性化仿生生物角膜的细菌合成

IF 18 1区医学 Q1 ENGINEERING, BIOMEDICAL

Bioactive Materials

Pub Date : 2025-12-30 DOI: 10.1016/j.bioactmat.2025.12.047

Liyin Wang , Luhan Bao , Zhanying Wang , Bingqing Sun , Yanze Yu , Yong Ma , Jiayi Hu , Xuan Ding , Yongle Bao , Junhui Dai , Zhe Zhang , Wenguo Cui , Xingtao Zhou , Jing Zhao

Corneal transplantation is one of the most effective treatments for corneal blindness. However, aside from clinical donor shortage, matching the transparency, curvature, and mechanical properties of artificial corneal materials with autologous corneas is challenging. This study employs bacterial synthetic biology, combining a curvature-customized model with an aldehyde modification system to develop a personalized biomimetic biological cornea (DBC@L-Cel) with a nanofiber network structure and customizable curvature and morphology. Dialdehyde bacterial nanocellulose (DBC) achieves a transmittance of 91.91 %, with optical and mechanical properties matching those of natural corneas. The incorporation of human-derived corneal lenticule microparticles (L) and Celastrol (Cel) significantly enhances the biocompatibility, adhesiveness, and anti-scarring capabilities of DBC. In vitro, DBC@L-Cel reduces stromal fibrosis by inhibiting the AGE/RAGE/NF-κB and PI3K/AKT pathway and activates epithelial proliferation and adhesion by upregulating the Wnt/β-catenin pathway. In vivo, transplantation of DBC@L-Cel significantly promotes the repair and regeneration of both the corneal stroma and epithelium, enabling rapid scarless reconstruction of the damaged cornea in a large-scale rabbit corneal defect model. This bacterially synthesized, curvature-customized, and highly transparent biomimetic cornea offers an innovative alternative for personalized transplantation therapy in corneal blindness.

角膜移植是治疗角膜失明最有效的方法之一。然而，除了临床供体短缺外，将人工角膜材料的透明度、曲率和力学性能与自体角膜相匹配是具有挑战性的。本研究采用细菌合成生物学，将曲率定制模型与醛修饰系统相结合，开发出具有纳米纤维网络结构、曲率和形态可定制的个性化仿生生物角膜（DBC@L-Cel）。双醛细菌纳米纤维素（DBC）的透过率达到91.91%，光学和机械性能与天然角膜相当。人源性角膜透镜微粒(L)和Celastrol （Cel）的掺入显著增强了DBC的生物相容性、黏附性和抗疤痕能力。在体外，DBC@L-Cel通过抑制AGE/RAGE/NF-κB和PI3K/AKT通路减少间质纤维化，通过上调Wnt/β-catenin通路激活上皮细胞增殖和粘附。在体内，DBC@L-Cel移植可显著促进角膜间质和上皮的修复和再生，使大面积兔角膜缺损模型中受损角膜快速无瘢痕重建。这种细菌合成的、曲率定制的、高度透明的仿生角膜为角膜失明的个性化移植治疗提供了一种创新的选择。

{"title":"Bacterial synthesis of personalized biomimetic biological cornea","authors":"Liyin Wang , Luhan Bao , Zhanying Wang , Bingqing Sun , Yanze Yu , Yong Ma , Jiayi Hu , Xuan Ding , Yongle Bao , Junhui Dai , Zhe Zhang , Wenguo Cui , Xingtao Zhou , Jing Zhao","doi":"10.1016/j.bioactmat.2025.12.047","DOIUrl":"10.1016/j.bioactmat.2025.12.047","url":null,"abstract":"<div><div>Corneal transplantation is one of the most effective treatments for corneal blindness. However, aside from clinical donor shortage, matching the transparency, curvature, and mechanical properties of artificial corneal materials with autologous corneas is challenging. This study employs bacterial synthetic biology, combining a curvature-customized model with an aldehyde modification system to develop a personalized biomimetic biological cornea (DBC@L-Cel) with a nanofiber network structure and customizable curvature and morphology. Dialdehyde bacterial nanocellulose (DBC) achieves a transmittance of 91.91 %, with optical and mechanical properties matching those of natural corneas. The incorporation of human-derived corneal lenticule microparticles (L) and Celastrol (Cel) significantly enhances the biocompatibility, adhesiveness, and anti-scarring capabilities of DBC. <em>In vitro</em>, DBC@L-Cel reduces stromal fibrosis by inhibiting the AGE/RAGE/NF-κB and PI3K/AKT pathway and activates epithelial proliferation and adhesion by upregulating the Wnt/β-catenin pathway. <em>In vivo</em>, transplantation of DBC@L-Cel significantly promotes the repair and regeneration of both the corneal stroma and epithelium, enabling rapid scarless reconstruction of the damaged cornea in a large-scale rabbit corneal defect model. This bacterially synthesized, curvature-customized, and highly transparent biomimetic cornea offers an innovative alternative for personalized transplantation therapy in corneal blindness.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"59 ","pages":"Pages 266-287"},"PeriodicalIF":18.0,"publicationDate":"2025-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145881749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell-only bioprinting of articular cartilage progenitor cells within a physically constraining support bath to engineer structurally organized grafts 关节软骨祖细胞在物理限制的支持浴中进行细胞生物打印，以设计结构组织的移植物

IF 18 1区医学 Q1 ENGINEERING, BIOMEDICAL

Bioactive Materials

Pub Date : 2025-12-29 DOI: 10.1016/j.bioactmat.2025.12.013

Aliaa S. Karam , Gabriela S. Kronemberger , Kaoutar Chattahy , Daniel J. Kelly

Engineering functional articular cartilage (AC) grafts is one of the greatest challenges in tissue engineering. Recapitulating the arcade-like collagen organisation of AC, which is integral to the tissues’ strength and stiffness, is necessary to engineer truly functional grafts. This motivates the need for innovative strategies to control collagen alignment in engineered tissues in a programmable manner. Emerging 3D bioprinting strategies can provide spatially defined cues to guide tissue growth. Therefore, the goal of this study was to use embedded bioprinting to provide spatially defined boundary conditions to AC progenitor cells (ACP) to direct collagen organization and support the development of biomimetic cartilage tissues. ACPs were isolated through differential adhesion to fibronectin and demonstrated superior chondrogenesis to donor matched chondrocytes. Two different approaches (casting and 3D bioprinting) were used to physically constrain ACPs with external boundaries of differing widths (250, 500, or 750 μm). For both approaches, thinner boundaries promoted greater collagen alignment along the long axis of the developing tissue. Building on this, ACPs were bioprinted into a sheet, with collagen fibers aligning parallel to the print direction. Finally, a multi-layered graft was bioprinted with horizontal filaments (XY plane) overlaying vertical filaments (Z-axis). The bioprinted tissue had an arcade-like collagen organization with horizontal collagen fibres overlaying vertical collagen fibres. These findings demonstrate how support baths can be used to provide spatially defined physical boundary conditions to bioprinted cells to guide matrix organization, enabling the engineering of anisotropic AC grafts.

工程功能关节软骨（AC）移植是组织工程中最大的挑战之一。概括AC的拱廊状胶原组织，这是组织强度和刚度的组成部分，是设计真正功能移植的必要条件。这激发了对创新策略的需求，以可编程的方式控制工程组织中的胶原排列。新兴的3D生物打印策略可以提供空间定义的线索来指导组织生长。因此，本研究的目的是利用嵌入式生物打印技术为AC祖细胞（ACP）提供空间定义的边界条件，以指导胶原组织并支持仿生软骨组织的发展。acp通过与纤维连接蛋白的不同粘附分离，并表现出比供体匹配的软骨细胞更优越的软骨形成。采用两种不同的方法（铸造和3D生物打印），以不同宽度（250、500或750 μm）的外部边界对acp进行物理约束。对于这两种方法，更薄的边界促进胶原蛋白沿着发育组织的长轴排列。在此基础上，acp被生物打印成薄片，胶原纤维与打印方向平行。最后，用水平丝（XY平面）覆盖垂直丝（z轴）的多层移植物进行生物打印。生物打印组织具有水平胶原纤维覆盖垂直胶原纤维的拱廊状胶原组织。这些发现表明，支撑液可以为生物打印细胞提供空间定义的物理边界条件，以指导基质组织，从而实现各向异性交流移植物的工程。

{"title":"Cell-only bioprinting of articular cartilage progenitor cells within a physically constraining support bath to engineer structurally organized grafts","authors":"Aliaa S. Karam , Gabriela S. Kronemberger , Kaoutar Chattahy , Daniel J. Kelly","doi":"10.1016/j.bioactmat.2025.12.013","DOIUrl":"10.1016/j.bioactmat.2025.12.013","url":null,"abstract":"<div><div>Engineering functional articular cartilage (AC) grafts is one of the greatest challenges in tissue engineering. Recapitulating the arcade-like collagen organisation of AC, which is integral to the tissues’ strength and stiffness, is necessary to engineer truly functional grafts. This motivates the need for innovative strategies to control collagen alignment in engineered tissues in a programmable manner. Emerging 3D bioprinting strategies can provide spatially defined cues to guide tissue growth. Therefore, the goal of this study was to use embedded bioprinting to provide spatially defined boundary conditions to AC progenitor cells (ACP) to direct collagen organization and support the development of biomimetic cartilage tissues. ACPs were isolated through differential adhesion to fibronectin and demonstrated superior chondrogenesis to donor matched chondrocytes. Two different approaches (casting and 3D bioprinting) were used to physically constrain ACPs with external boundaries of differing widths (250, 500, or 750 μm). For both approaches, thinner boundaries promoted greater collagen alignment along the long axis of the developing tissue. Building on this, ACPs were bioprinted into a sheet, with collagen fibers aligning parallel to the print direction. Finally, a multi-layered graft was bioprinted with horizontal filaments (XY plane) overlaying vertical filaments (Z-axis). The bioprinted tissue had an arcade-like collagen organization with horizontal collagen fibres overlaying vertical collagen fibres. These findings demonstrate how support baths can be used to provide spatially defined physical boundary conditions to bioprinted cells to guide matrix organization, enabling the engineering of anisotropic AC grafts.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"59 ","pages":"Pages 251-265"},"PeriodicalIF":18.0,"publicationDate":"2025-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145881748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly bio-adapted hydrogels for tendon-bone interface regeneration: Natural healing inspiration, design strategies, and biomedical potential 用于肌腱-骨界面再生的高度生物适应性水凝胶：自然愈合灵感、设计策略和生物医学潜力

IF 18 1区医学 Q1 ENGINEERING, BIOMEDICAL

Bioactive Materials

Pub Date : 2025-12-29 DOI: 10.1016/j.bioactmat.2025.12.043

Xinghao Yin , Juehong Li , Cunyi Fan

Against the dual backdrop of a global push to promote physical activity and the progressive degeneration of the musculoskeletal system due to aging, a significant imbalance has emerged between life expectancy and quality of life. Tendon–bone interface (TBI) injuries markedly impair physical function and overall well-being. The anatomical gradient structure of the TBI, along with the spatiotemporal complexity of its cellular composition and distribution, poses substantial challenges to postoperative healing. This review examines the vulnerability of the TBI under physiological conditions, the spatial gradient distribution of various functional cell types, and the concentration gradients of cytokines. We further introduce the reparative processes that occur following TBI injury and highlight key strategies for interface regeneration. In recent years, advances in tissue engineering have endowed hydrogels with unique biological properties and potential to mimic the gradient architecture of native TBI tissue, making them promising candidates for TBI repair and thereby improving clinical outcomes. We categorize current hydrogel-based strategies for enhancing TBI healing into four main types: improving hydrogel physicochemical properties, mimicking native anatomical structures, replicating dynamic gradients of cells and cytokines, and responding adaptively to the healing microenvironment. Lastly, we discuss the selection of functional cells and the mechanisms through which bioactive factors contribute to TBI regeneration. In summary, this review provides insights into the design of highly bio-adapted hydrogels tailored to the gradient structure and biological property of the TBI and offers guidance for future research on hydrogel-based therapeutic strategies.

在全球推动身体活动和衰老导致肌肉骨骼系统进行性退化的双重背景下，预期寿命和生活质量之间出现了严重的不平衡。肌腱-骨界面（TBI）损伤明显损害身体功能和整体健康。TBI的解剖梯度结构，以及其细胞组成和分布的时空复杂性，对术后愈合提出了重大挑战。本文综述了脑损伤在生理条件下的易损性、各种功能细胞类型的空间梯度分布以及细胞因子的浓度梯度。我们进一步介绍了脑损伤后的修复过程，并强调了界面再生的关键策略。近年来，组织工程的进展赋予了水凝胶独特的生物学特性和模仿天然TBI组织梯度结构的潜力，使其成为TBI修复的有希望的候选者，从而改善临床结果。我们将目前用于增强创伤性脑损伤愈合的基于水凝胶的策略分为四种主要类型：改善水凝胶的物理化学性质，模仿天然解剖结构，复制细胞和细胞因子的动态梯度，以及对愈合微环境的适应性响应。最后，我们讨论了功能细胞的选择和生物活性因子促进TBI再生的机制。综上所述，本综述为设计适合TBI梯度结构和生物学特性的高度生物适应性水凝胶提供了见解，并为未来基于水凝胶的治疗策略研究提供了指导。

{"title":"Highly bio-adapted hydrogels for tendon-bone interface regeneration: Natural healing inspiration, design strategies, and biomedical potential","authors":"Xinghao Yin , Juehong Li , Cunyi Fan","doi":"10.1016/j.bioactmat.2025.12.043","DOIUrl":"10.1016/j.bioactmat.2025.12.043","url":null,"abstract":"<div><div>Against the dual backdrop of a global push to promote physical activity and the progressive degeneration of the musculoskeletal system due to aging, a significant imbalance has emerged between life expectancy and quality of life. Tendon–bone interface (TBI) injuries markedly impair physical function and overall well-being. The anatomical gradient structure of the TBI, along with the spatiotemporal complexity of its cellular composition and distribution, poses substantial challenges to postoperative healing. This review examines the vulnerability of the TBI under physiological conditions, the spatial gradient distribution of various functional cell types, and the concentration gradients of cytokines. We further introduce the reparative processes that occur following TBI injury and highlight key strategies for interface regeneration. In recent years, advances in tissue engineering have endowed hydrogels with unique biological properties and potential to mimic the gradient architecture of native TBI tissue, making them promising candidates for TBI repair and thereby improving clinical outcomes. We categorize current hydrogel-based strategies for enhancing TBI healing into four main types: improving hydrogel physicochemical properties, mimicking native anatomical structures, replicating dynamic gradients of cells and cytokines, and responding adaptively to the healing microenvironment. Lastly, we discuss the selection of functional cells and the mechanisms through which bioactive factors contribute to TBI regeneration. In summary, this review provides insights into the design of highly bio-adapted hydrogels tailored to the gradient structure and biological property of the TBI and offers guidance for future research on hydrogel-based therapeutic strategies.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"59 ","pages":"Pages 224-250"},"PeriodicalIF":18.0,"publicationDate":"2025-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145881747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A promising magnesium-related alloy with metabolic reprogramming and antitumor effects in hepatocellular and pancreatic cancer 一种在肝细胞癌和胰腺癌中具有代谢重编程和抗肿瘤作用的镁相关合金

IF 18 1区医学 Q1 ENGINEERING, BIOMEDICAL

Bioactive Materials

Pub Date : 2025-12-27 DOI: 10.1016/j.bioactmat.2025.12.039

Junfeng Zhang , Jianyou Gu , Renpei Xia , Jingyang Yin , Xianxing Wang , Jiali Yang , Yanjun Wang , Zhongchao Yi , Senwei Wang , Qifan Zhang , Huaizhi Wang , Jia She , Shixiang Guo

Hepatocellular and pancreatic cancers are highly aggressive malignancies with dismal clinical outcomes, highlighting an urgent need for new therapeutic strategies. Magnesium-related alloys, widely explored for their biocompatibility and bioactivity, are attractive candidates for biliary and pancreatic duct stents. However, their antitumor potential and underlying mechanisms remain incompletely defined. Here, we systematically characterized the physicochemical properties and anticancer activities of a panel of magnesium-related alloy powders and identified an aluminum-magnesium (Al-Mg) alloy as the most potent candidate. Compared with pure Mg, Al-Mg rods exhibited stronger antitumor efficacy together with more controllable degradation. In vitro and in vivo assays confirmed that Al-Mg significantly inhibited hepatocellular carcinoma and pancreatic cancer progression. Integrated metabolomic and transcriptomic analyses indicated that Al-Mg activates AMPK signaling and suppresses purine and pyrimidine metabolism, consistent with metabolic reprogramming that limits tumor cell proliferation. Furthermore, single-cell and spatial transcriptomic analyses delineated Al-Mg-sensitive tumor cell subpopulations and mapped their spatial distribution within pancreatic cancer tissues. Collectively, these findings position Al-Mg as a promising antitumor material and provide a mechanistic framework supporting the development of magnesium-related alloys for local oncologic intervention.

肝细胞癌和胰腺癌是高度侵袭性的恶性肿瘤，临床结果令人沮丧，迫切需要新的治疗策略。镁合金因其生物相容性和生物活性而被广泛研究，是胆管和胰管支架有吸引力的候选者。然而，它们的抗肿瘤潜能和潜在机制仍未完全确定。在这里，我们系统地表征了一组镁相关合金粉末的物理化学性质和抗癌活性，并确定了一种铝镁（Al-Mg）合金是最有效的候选者。与纯Mg相比，Al-Mg棒具有更强的抗肿瘤作用，且降解更可控。体外和体内实验证实，Al-Mg显著抑制肝细胞癌和胰腺癌的进展。综合代谢组学和转录组学分析表明，Al-Mg激活AMPK信号并抑制嘌呤和嘧啶代谢，与限制肿瘤细胞增殖的代谢重编程一致。此外，单细胞和空间转录组学分析描绘了al - mg敏感的肿瘤细胞亚群，并绘制了它们在胰腺癌组织中的空间分布。总的来说，这些发现表明Al-Mg是一种很有前途的抗肿瘤材料，并为局部肿瘤干预中镁相关合金的开发提供了机制框架。

{"title":"A promising magnesium-related alloy with metabolic reprogramming and antitumor effects in hepatocellular and pancreatic cancer","authors":"Junfeng Zhang , Jianyou Gu , Renpei Xia , Jingyang Yin , Xianxing Wang , Jiali Yang , Yanjun Wang , Zhongchao Yi , Senwei Wang , Qifan Zhang , Huaizhi Wang , Jia She , Shixiang Guo","doi":"10.1016/j.bioactmat.2025.12.039","DOIUrl":"10.1016/j.bioactmat.2025.12.039","url":null,"abstract":"<div><div>Hepatocellular and pancreatic cancers are highly aggressive malignancies with dismal clinical outcomes, highlighting an urgent need for new therapeutic strategies. Magnesium-related alloys, widely explored for their biocompatibility and bioactivity, are attractive candidates for biliary and pancreatic duct stents. However, their antitumor potential and underlying mechanisms remain incompletely defined. Here, we systematically characterized the physicochemical properties and anticancer activities of a panel of magnesium-related alloy powders and identified an aluminum-magnesium (Al-Mg) alloy as the most potent candidate. Compared with pure Mg, Al-Mg rods exhibited stronger antitumor efficacy together with more controllable degradation. <em>In vitro</em> and <em>in vivo</em> assays confirmed that Al-Mg significantly inhibited hepatocellular carcinoma and pancreatic cancer progression. Integrated metabolomic and transcriptomic analyses indicated that Al-Mg activates AMPK signaling and suppresses purine and pyrimidine metabolism, consistent with metabolic reprogramming that limits tumor cell proliferation. Furthermore, single-cell and spatial transcriptomic analyses delineated Al-Mg-sensitive tumor cell subpopulations and mapped their spatial distribution within pancreatic cancer tissues. Collectively, these findings position Al-Mg as a promising antitumor material and provide a mechanistic framework supporting the development of magnesium-related alloys for local oncologic intervention.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"59 ","pages":"Pages 186-204"},"PeriodicalIF":18.0,"publicationDate":"2025-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145838819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tailoring nanotopography and antibacterial properties of calcium phosphate bone grafts via fluoride incorporation 通过氟化物掺入调整磷酸钙骨移植物的纳米形貌和抗菌性能

IF 18 1区医学 Q1 ENGINEERING, BIOMEDICAL

Bioactive Materials

Pub Date : 2025-12-27 DOI: 10.1016/j.bioactmat.2025.12.026

Carla Arca-Garcia , Maria Godoy-Gallardo , Maria-Pau Ginebra

Despite advances in bone graft design and surgical techniques, bacterial infection remains a major cause of graft failure, exacerbated by the global rise in antimicrobial resistance. This has intensified the pursuit of antibiotic-free strategies to prevent bacterial colonization. Among these, antibacterial surface nanotopographies have emerged as promising tools, leveraging nanoscale geometries to physically disrupt bacteria upon contact. In this study, we engineered the surface of a calcium phosphate bone graft to confer antimicrobial functionality through a dual approach: the creation of high-aspect-ratio nanotopographies and ionic doping with fluoride. Through controlled hydrolysis of α-tricalcium phosphate by biomimetic and hydrothermal treatments, we generated calcium deficient hydroxyapatite nanoneedle structures whose morphology and biofunctionality were tuned via fluoride incorporation. XRD and Raman spectroscopy confirmed the formation of hydroxy-fluorapatite, with phase composition and surface morphology dependent on fluoride concentration and processing parameters. Fluoride doping significantly altered nanoneedle dimensions and spacing and enhanced bactericidal activity, particularly against P. aeruginosa, and to a lesser extent S. aureus. Notably, fluoride-doping alone showed no antibacterial effects; however, when combined with nanotopography, a synergistic increase in efficacy was observed. Importantly, the antimicrobial surfaces supported the proliferation and osteogenic differentiation of SaOS-2 cells. Co-culture assays modeling pre- and post-implantation infection scenarios demonstrated robust cell adhesion and markedly reduced bacterial colonization. In conclusion, our findings present a multifunctional, synthetic bone graft with both physical and chemical antibacterial properties, offering a promising strategy to mitigate infection risks while supporting osteointegration.

尽管骨移植设计和手术技术取得了进步，但细菌感染仍然是导致骨移植失败的主要原因，全球抗菌素耐药性的上升加剧了这种情况。这加强了对无抗生素策略的追求，以防止细菌定植。其中，抗菌表面纳米形貌已成为有前途的工具，利用纳米级几何形状在接触时物理破坏细菌。在这项研究中，我们设计了磷酸钙骨移植物的表面，通过双重方法赋予抗菌功能：创建高纵横比纳米形貌和氟离子掺杂。通过仿生和水热处理对α-磷酸三钙的水解，我们得到了缺乏钙的羟基磷灰石纳米针结构，其形态和生物功能通过氟化物的掺入进行了调整。XRD和拉曼光谱证实了羟基氟磷灰石的形成，其相组成和表面形貌取决于氟化物浓度和加工参数。氟掺杂显著改变了纳米针的尺寸和间距，增强了杀菌活性，特别是对铜绿假单胞菌，在较小程度上对金黄色葡萄球菌。值得注意的是，单独掺杂氟化物没有抗菌效果；然而，当与纳米形貌相结合时，观察到药效的协同增加。重要的是，抗菌表面支持SaOS-2细胞的增殖和成骨分化。模拟植入前和植入后感染情景的共培养实验表明，细胞粘附能力强，细菌定植明显减少。总之，我们的研究结果提出了一种具有物理和化学抗菌特性的多功能合成骨移植物，为减轻感染风险同时支持骨整合提供了一种有希望的策略。

{"title":"Tailoring nanotopography and antibacterial properties of calcium phosphate bone grafts via fluoride incorporation","authors":"Carla Arca-Garcia , Maria Godoy-Gallardo , Maria-Pau Ginebra","doi":"10.1016/j.bioactmat.2025.12.026","DOIUrl":"10.1016/j.bioactmat.2025.12.026","url":null,"abstract":"<div><div>Despite advances in bone graft design and surgical techniques, bacterial infection remains a major cause of graft failure, exacerbated by the global rise in antimicrobial resistance. This has intensified the pursuit of antibiotic-free strategies to prevent bacterial colonization. Among these, antibacterial surface nanotopographies have emerged as promising tools, leveraging nanoscale geometries to physically disrupt bacteria upon contact. In this study, we engineered the surface of a calcium phosphate bone graft to confer antimicrobial functionality through a dual approach: the creation of high-aspect-ratio nanotopographies and ionic doping with fluoride. Through controlled hydrolysis of α-tricalcium phosphate by biomimetic and hydrothermal treatments, we generated calcium deficient hydroxyapatite nanoneedle structures whose morphology and biofunctionality were tuned via fluoride incorporation. XRD and Raman spectroscopy confirmed the formation of hydroxy-fluorapatite, with phase composition and surface morphology dependent on fluoride concentration and processing parameters. Fluoride doping significantly altered nanoneedle dimensions and spacing and enhanced bactericidal activity, particularly against <em>P. aeruginosa</em>, and to a lesser extent <em>S. aureus</em>. Notably, fluoride-doping alone showed no antibacterial effects; however, when combined with nanotopography, a synergistic increase in efficacy was observed. Importantly, the antimicrobial surfaces supported the proliferation and osteogenic differentiation of SaOS-2 cells. Co-culture assays modeling pre- and post-implantation infection scenarios demonstrated robust cell adhesion and markedly reduced bacterial colonization. In conclusion, our findings present a multifunctional, synthetic bone graft with both physical and chemical antibacterial properties, offering a promising strategy to mitigate infection risks while supporting osteointegration.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"59 ","pages":"Pages 205-223"},"PeriodicalIF":18.0,"publicationDate":"2025-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145838749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An embolism-free nonfouling hydrogel coating with high toughness and lubricity for intravascular medical devices via chain-entanglement mediated topological gelation 一种通过链缠结介导的拓扑凝胶，用于血管内医疗器械的无栓塞、无污垢、高韧性和润滑性的水凝胶涂层

IF 18 1区医学 Q1 ENGINEERING, BIOMEDICAL

Bioactive Materials

Pub Date : 2025-12-26 DOI: 10.1016/j.bioactmat.2025.11.033

Wenzhong Cao , Xianchi Zhou , Wenbin Dai , Zihao Zhu , Zuolong Liu , Kexin Chen , Yu Yan , Hengshuai Bao , Jian Ji , Peng Zhang

Hydrogel coatings have become indispensable for advanced interventional devices owing to their exceptional lubricity, antithrombogenicity, and antibacterial performance. However, clinical adoption has been hindered by the risk of polymer embolism resulting from coating delamination in vascular systems. Herein, we report an embolism-free hydrogel coating strategy based on chain-entanglement-mediated topological gelation to address this limitation. Through an interface-regulated polymerization process, we fabricate a robust zwitterionic hydrogel coating stabilized by interfacial interlinking and enhanced polymer chain entanglement. This design delivers outstanding long-term antimicrobial and antithrombogenic properties and achieves high toughness, ultra-low friction, and wear resistance. Notably, the chain-entangled topological hydrogel exhibits a unique fail-safe mechanism: upon detachment, it undergoes spontaneous aqueous dissolution rather than forming hazardous debris. This approach can potentially reduce life-threatening complications in interventional therapies by eliminating the risk of polymer-induced embolism.

由于其优异的润滑性、抗血栓性和抗菌性能，水凝胶涂层已成为先进介入设备不可或缺的材料。然而，由于血管系统涂层分层导致聚合物栓塞的风险，临床应用一直受到阻碍。在此，我们报告了一种基于链缠结介导的拓扑凝胶的无栓塞水凝胶涂层策略来解决这一限制。通过界面调节聚合工艺，我们制备了一种坚固的两性离子水凝胶涂层，该涂层通过界面互连和增强聚合物链纠缠来稳定。这种设计提供了出色的长期抗菌和抗血栓形成性能，并实现了高韧性，超低摩擦和耐磨性。值得注意的是，链缠绕的拓扑水凝胶表现出一种独特的故障安全机制：在脱离时，它经历了自发的水溶解，而不是形成危险的碎片。这种方法可以通过消除聚合物诱发栓塞的风险，潜在地减少介入治疗中危及生命的并发症。

{"title":"An embolism-free nonfouling hydrogel coating with high toughness and lubricity for intravascular medical devices via chain-entanglement mediated topological gelation","authors":"Wenzhong Cao , Xianchi Zhou , Wenbin Dai , Zihao Zhu , Zuolong Liu , Kexin Chen , Yu Yan , Hengshuai Bao , Jian Ji , Peng Zhang","doi":"10.1016/j.bioactmat.2025.11.033","DOIUrl":"10.1016/j.bioactmat.2025.11.033","url":null,"abstract":"<div><div>Hydrogel coatings have become indispensable for advanced interventional devices owing to their exceptional lubricity, antithrombogenicity, and antibacterial performance. However, clinical adoption has been hindered by the risk of polymer embolism resulting from coating delamination in vascular systems. Herein, we report an embolism-free hydrogel coating strategy based on chain-entanglement-mediated topological gelation to address this limitation. Through an interface-regulated polymerization process, we fabricate a robust zwitterionic hydrogel coating stabilized by interfacial interlinking and enhanced polymer chain entanglement. This design delivers outstanding long-term antimicrobial and antithrombogenic properties and achieves high toughness, ultra-low friction, and wear resistance. Notably, the chain-entangled topological hydrogel exhibits a unique fail-safe mechanism: upon detachment, it undergoes spontaneous aqueous dissolution rather than forming hazardous debris. This approach can potentially reduce life-threatening complications in interventional therapies by eliminating the risk of polymer-induced embolism.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"59 ","pages":"Pages 174-185"},"PeriodicalIF":18.0,"publicationDate":"2025-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145838820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0