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Modeling the Thermal Inactivation of Ascospores from Heat-Resistant Molds in Pineapple Juice and Evaluating Disinfection Efficiency of Sodium Hypochlorite and Chlorine Dioxide 菠萝汁中耐热霉菌子囊孢子热失活模拟及次氯酸钠和二氧化氯消毒效果评价
Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-11-14 DOI: 10.3390/beverages9040096
Thanapoom Maneeboon, Somsiri Sangchote, Ratchanee Hongprayoon, Chananya Chuaysrinule, Warapa Mahakarnchanakul
The contamination and spoilage of heat-treated fruit juices by heat-resistant mold ascospores present significant challenges to the food industry. Understanding effective strategies to mitigate this contamination is vital for ensuring the shelf-life and microbial safety of heat-treated fruit juices. This study investigated the thermal resistance of ascospores from different heat-resistant mold species, including Aspergillus laciniosus, A. chevalieri, A. denticulatus, A. siamensis, Hamigera pallida, and Talaromyces macrosporus, isolated from pineapple and sugarcane field soils. Ascospores inactivation kinetics in pineapple juice under heat treatment (75–97 °C) were analyzed using log-linear and Weibull models. Among these species, A. laciniosus displayed the highest heat resistance (δ-value: 104.59 min at 85 °C), while A. siamensis exhibited the lowest (δ-value: 3.39 min at 80 °C). Furthermore, A. laciniosus, the most heat-resistant species, showed notable tolerance to sanitizers. The most effective inactivation was achieved using 1.0% (w/v) sodium hypochlorite for 15 min. Chlorine dioxide, however, was generally ineffective and even activated dormant ascospores in some cases. The combination of hot water (65 °C for 5 min) with sanitizer increased ascospore reduction in most species but did not achieve the 3-log reduction required by the European Standard N13697. This study revealed a correlation between ascospore resistance to heat and chlorine dioxide, offering significant findings for practical inactivation strategies.
耐热霉菌子囊孢子对热处理果汁的污染和破坏对食品工业提出了重大挑战。了解减少这种污染的有效策略对于确保热处理果汁的保质期和微生物安全至关重要。本研究研究了从菠萝和甘蔗田土壤中分离的不同耐热霉菌,包括曲霉(Aspergillus laciniosus)、A. chevalieri、A. denticulatus、A. siamensis、Hamigera pallida和Talaromyces macrosporus)的子囊孢子的耐热性。采用对数线性和威布尔模型分析了菠萝汁热处理(75 ~ 97℃)时子囊孢子失活动力学。其中,裂唇竹的耐热性最高(85℃时的δ值为104.59 min),气孔竹的耐热性最低(80℃时的δ值为3.39 min)。此外,最耐热的品种laciniosus对消毒剂表现出显著的耐受性。使用1.0% (w/v)的次氯酸钠灭活15分钟是最有效的。然而,二氧化氯通常无效,甚至在某些情况下激活了休眠的子囊孢子。热水(65°C 5分钟)与消毒剂的组合增加了大多数品种的子囊孢子减少,但没有达到欧洲标准N13697所要求的3对数减少。本研究揭示了子囊孢子耐热性与二氧化氯之间的相关性,为实际灭活策略提供了重要的发现。
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引用次数: 0
Possibilities for Utilization of Cherry Products (Juice and Pomace) in Beer Production 樱桃产品(果汁和果渣)在啤酒生产中的应用可能性
Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-11-10 DOI: 10.3390/beverages9040095
Petar Nedyalkov, Ivan Bakardzhiyski, Vasil Shikov, Maria Kaneva, Vesela Shopska
Fruit addition can enrich beer with flavor and bioactive substances. Sweet cherry (Prunus avium L.) can be added in beer as a whole fruit, fruit juice, or pulp, but there is no data for the addition of cherry pomace in beer. Therefore, we investigated the addition of cherry juice and pomace during beer fermentation on the first and seventh day and studied the basic beer parameters (alcohol and extract), sensorial evaluation, phenolic compounds, and antioxidant activity of the beers produced, measured using six different methods (DPPH, ABTS, FRAP, CUPRAC, ORAC, and HORAC) and compared the results with a control sample without cherry products addition. The results showed a strong correlation between the antioxidant activity values obtained using the DPPH, FRAP, CUPRAC, and HORAC methods and the concentration of phenolic compounds in the studied beers. The phenolic compound content and antioxidant activity increased when cherries juice or pomace were added. The increase was much more significant when pomace was used. Therefore, it can be concluded that cherry pomace addition is a better option than cherry juice for beer production because of the increased content of bioactive compounds and the sustainability of the beers obtained.
添加水果可以丰富啤酒的风味和生物活性物质。甜樱桃(Prunus avium L.)可以作为整个水果、果汁或果肉添加到啤酒中,但没有在啤酒中添加樱桃渣的数据。因此,我们在啤酒发酵的第1天和第7天研究了樱桃汁和渣的添加,研究了啤酒的基本参数(酒精和提取物)、感官评价、酚类化合物和啤酒的抗氧化活性,使用6种不同的方法(DPPH、ABTS、FRAP、CUPRAC、ORAC和HORAC)进行了测量,并将结果与不添加樱桃产品的对照样品进行了比较。结果表明,采用DPPH、FRAP、CUPRAC和HORAC方法获得的抗氧化活性值与所研究啤酒中酚类化合物的浓度之间存在很强的相关性。添加樱桃汁或果渣均可提高其酚类化合物含量和抗氧化活性。当使用渣土时,这种增加要显著得多。因此,与樱桃汁相比,添加樱桃渣可以提高啤酒的生物活性成分含量和啤酒的可持续性。
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引用次数: 0
The Impact of Different Withering Process Conditions on the Bioactivity and Quality of Black Tea from Azorean Camellia sinensis 不同萎凋工艺条件对亚速尔山茶生物活性及品质的影响
Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-11-09 DOI: 10.3390/beverages9040094
Lisete Sousa Paiva, Ana Paula Dias, Massimo Francesco Marcone, José António Bettencourt Baptista
The objective of this study was to investigate the variability of natural bioactive compounds, such as catechin, theaflavin, total phenolic content (TPC), and total flavonoid content (TFC), of Azorean black tea (Camellia sinensis L., O. Kuntze) as well as its antioxidant activities according to different withering times. The TPC, TFC, free radical scavenging activity (FRSA), ferric reducing antioxidant power (FRAP), and ferrous-ion-chelating (FIC) activities were determined by colorimetric methods, and catechin and theaflavin contents were analyzed by high-pressure liquid chromatography (HPLC). The FRSA shows similar results for the withering range of 6 to 16 h (hours). For FRAP, the best results were observed at 16 h, and for FIC, the highest value was at 20 h. The TPC and TFC showed the highest value at 9 h and the lowest at 20 h. For the total theaflavins, the highest results were obtained after 12 h of withering, and the lowest values were obtained at 16 and 20 h. According to the different withering times, the highest value of total catechin levels was at 12 h, while the lowest value was observed at 20 h. Regarding caffeine content, all samples presented similar results, with the exception of the 12 h time point. In conclusion, the best withering times were observed in the range of 9 to 16 h, showing decreased values at 20 h, with the exception of FIC.
摘要研究了亚速尔红茶(Camellia sinensis L., O. Kuntze)天然活性物质儿茶素、茶黄素、总酚含量(TPC)和总黄酮含量(TFC)随萎凋时间的变化及其抗氧化活性。用比色法测定其TPC、TFC、自由基清除能力(FRSA)、铁还原抗氧化能力(FRAP)和铁离子螯合能力(FIC),用高压液相色谱法(HPLC)测定其儿茶素和茶黄素含量。FRSA在6 ~ 16 h的凋萎过程中也显示出类似的结果。收紧,最好的结果观察到16 h, FIC,最高价值是在20 h。TPC和薄膜电路显示最高的价值在9 h和最低20 h。总茶黄素,最高12 h的结果后,和最低的价值得到16岁和20 h。根据不同的时间,最高的价值总额的儿茶素水平在12 h,而价值最低的是关于咖啡因含量,观察到20 h。除了12 h时间点外,所有样本的结果都相似。综上所述,除FIC外,9 ~ 16 h为最佳枯死时间,20 h枯死时间逐渐减少。
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引用次数: 0
The Impact of Rye and Barley Malt and Different Strains of Saccharomyces cerevisiae on Beer Volatilome 黑麦、大麦麦芽及不同酿酒酵母菌对啤酒挥发物的影响
Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-11-08 DOI: 10.3390/beverages9040093
Noemi Tocci, Gian Marco Riccio, Abirami Ramu Ganesan, Philipp Hoellrigl, Peter Robatscher, Lorenza Conterno
Craft breweries are continuously searching for beers made with locally produced raw materials and unique flavor profiles to respond to consumer requests. We explored the behavior of three commercial strains of Saccharomyces cerevisiae in the fermentation of ale beer with a high prevalence of rye malt in comparison to pure barley malt. In total, 34 volatile organic compounds were identified, with esters and alcohols being the quantitatively most abundant classes. The yeast strain appeared to impart more differences in the beer’s volatile profile compared to malt. In particular, S. cerevisiae var. diastaticus Y2 strain was associated with a higher production of esters, while strain S. cerevisiae Y3 was correlated to the higher amounts of terpenes together with the lowest relative abundance of volatile acids. Our findings encourage further investigation of the fermentation performance of several yeast strains to produce beers with unique flavors.
精酿啤酒厂一直在寻找用当地生产的原料和独特风味酿造的啤酒,以满足消费者的需求。我们探索了三种商业酿酒酵母在黑麦麦芽与纯大麦麦芽高流行率的啤酒发酵中的行为。总共鉴定出34种挥发性有机化合物,其中酯类和醇类是数量最多的类别。与麦芽相比,酵母菌株似乎在啤酒的挥发性特征上产生了更大的差异。其中,酿酒葡萄球菌(S. cerevisiae var. disastaticus) Y2菌株的酯类含量较高,而酿酒葡萄球菌(S. cerevisiae) Y3菌株的萜烯含量较高,挥发性酸的相对丰度最低。我们的发现鼓励进一步研究几种酵母菌株的发酵性能,以生产具有独特风味的啤酒。
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引用次数: 0
Phenolic Compounds Stability of Grumixama (Eugenia brasiliensis) Juice during Processing and Storage 柚子汁加工和贮存过程中酚类化合物的稳定性
Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-11-07 DOI: 10.3390/beverages9040091
Elivaldo Nunes Modesto Junior, Rosane Patricia Ferreira Chaves, Mayara Galvão Martins, Gustavo Araujo Pereira, Renan Campos Chisté, Rosinelson da Silva Pena
Grumixama is an anthocyanin-rich berry commonly found in South America, yet not widely consumed by Brazilians due to the lack of studies covering its technological properties. Therefore, the stability of anthocyanins and the activity of oxidoreductase enzymes in grumixama juice during thermal treatment (60 °C to 100 °C) and storage (at 25 °C and 7 °C) were evaluated. As a result, the pasteurization of grumixama juice at 80 °C for 60 s completely inactivated peroxidase (POD) and polyphenol oxidase (PPO), eliminated microorganisms, and induced low degradation of the total anthocyanins (40%) and minimal degradation of the total phenolic compounds. Four anthocyanins were monitored in the pasteurized juice, namely delphinidin 3-glucoside, cyanidin 3-glucoside, and two unidentified anthocyanins. Despite the temperature range, the qualitative chromatographic profile of the anthocyanins did not change, while the contents were affected. The binominal temperature/time of 80 °C/60 s effectively inactivated PPO and POD enzymes, promoted moderate alterations in the contents of total anthocyanins and total phenolic compounds, and ensured the microbiological quality of grumixama juice. Grumixama juice can be used as a health food due to its high content of antioxidant phenolic compounds, especially anthocyanins.
Grumixama是一种富含花青素的浆果,常见于南美洲,但由于缺乏对其技术特性的研究,巴西人并未广泛食用。因此,我们对热处理(60°C ~ 100°C)和贮藏(25°C和7°C)期间花青素的稳定性和氧化还原酶的活性进行了评价。结果表明,在80°C条件下进行60 s的巴氏灭菌,可以完全灭活过氧化物酶(POD)和多酚氧化酶(PPO),消除微生物,诱导总花青素的低降解(40%)和总酚类化合物的最低降解。在巴氏灭菌果汁中检测了四种花青素,即飞鸽苷3-葡萄糖苷、花青素3-葡萄糖苷和两种未识别的花青素。在不同的温度范围内,花青素的质谱特征没有变化,但含量受到影响。80℃/60 s的二项温度/时间能有效灭活PPO和POD酶,促进总花青素和总酚类化合物含量的适度变化,保证了枇杷汁的微生物品质。由于其抗氧化酚类化合物,特别是花青素含量高,因此可以作为保健食品使用。
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引用次数: 0
Identification of the Mixing Ratios of Beer-Mixed Beverages by UV/VIS Spectroscopy 用紫外/可见光谱法鉴别啤酒混合饮料的混合比例
Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-11-07 DOI: 10.3390/beverages9040092
Sophia Stinus, Pauline Erban, Josef Diebold, Magnus S. Schmidt
UV/VIS spectroscopy was used in conjunction with a calibration method to determine the mixing ratio of samples of beer-mixed drinks with mineral water (sour Radler beers) from the food service industry. For this purpose, calibration lines were constructed based on absorptions at the two wavelengths 380 nm and 430 nm. To create these calibration lines, blending ratios were prepared from beers from three southwestern German brands and mineral water. The samples of the sour Radler beers were taken from restaurants and bars, which are all located in Baden-Württemberg. It has been shown that the calibration lines can be used to determine mixing ratios with an average deviation of approximately 7% and 9%. Thereby, the calibration line based on the absorption at 430 nm represents a higher accuracy. Using this calibration line, the sour Radler beer samples have percentage ranges of beer of approximately 50% to 90%. This method could serve as a rapid quantitative quality control of the mixing ratios of sour Radler or other beer-mixed drinks. It could be used in breweries for routine control.
紫外/可见光谱学与校准方法相结合,用于确定食品服务行业啤酒混合饮料样品与矿泉水(酸拉德勒啤酒)的混合比例。为此,基于380 nm和430 nm两个波长的吸收构建了校准线。为了创建这些校准线,配制了来自德国西南部三个品牌的啤酒和矿泉水的混合比例。酸味拉德勒啤酒的样品取自巴登-符腾堡州的餐馆和酒吧。结果表明,标定线可用于测定混合比,平均偏差约为7%和9%。因此,基于430 nm吸收的校准线具有更高的精度。使用这条校准线,酸拉德勒啤酒样品的啤酒百分比范围约为50%至90%。该方法可作为酸拉德勒或其他啤酒混合饮料混合比例的快速定量质量控制。它可以用于啤酒厂的日常控制。
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引用次数: 0
Buffalo Whey-Based Cocoa Beverages with Unconventional Plant-Based Flours: The Effect of Information and Taste on Consumer Perception 水牛乳清可可饮料与非常规植物基面粉:信息和味道对消费者感知的影响
Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-10-31 DOI: 10.3390/beverages9040090
Madian Johel Galo Salgado, Iuri Lima dos Santos Rosario, Arlen Carvalho de Oliveira Almeida, Bruna Samara dos Santos Rekowsky, Uiara Moreira Paim, Deborah Murowaniecki Otero, Maria Eugênia de Oliveira Mamede, Marion Pereira da Costa
This study aimed to evaluate the addition of açaí, beetroot, and hibiscus flour on the sensory characteristics of a buffalo whey-based cocoa beverage and, second, to consider if health and sustainability claims could enhance consumer acceptance and purchase intention for the buffalo whey-based cocoa beverage. In this sense, five treatments were elaborated; BCC, the control with a commercial beverage formulation; BCE, the experimental control; BFA, with the addition of açaí flour; BFB, with added beetroot flour; and BFH, with the addition of hibiscus flour. The experiment was divided into two stages: In the first, the beverages were submitted to sensory analyses of acceptance (nine-point hedonic scale), purchase intention and just-about-right (five points), and check-all-that-apply (CATA). In the second stage, the beverages with the highest and lowest acceptance rates were taken, and they were subjected to the effect of sustainability and health information on consumer acceptance, purchase intention, and the CATA test using terms referring to emotions and feelings. The addition of flours decreased the beverage acceptance rate compared to the BCC treatment. The treatments were penalized in aroma and sweet taste. There was no effect on the type of information received by the consumer. Probably, the addition of high cocoa percentages can negatively affect the acceptance of products, as well as the use of flour with bitter flavors, due to the greater acceptance of sweeter products.
本研究旨在评估açaí、甜菜根和芙蓉粉对水牛乳清基可可饮料感官特性的影响,其次,考虑健康和可持续性声明是否能提高消费者对水牛乳清基可可饮料的接受度和购买意愿。在这个意义上,阐述了五种处理方法;BCC,对照用商业饮料配方;BCE,实验对照;BFA,加入açaí面粉;BFB,加入甜菜根粉;和BFH,加入芙蓉粉。实验分为两个阶段:首先,对饮料进行感官分析,包括接受度(9分享乐量表)、购买意愿和刚刚好(5分),以及所有适用的检查(CATA)。在第二阶段,选取接受率最高和最低的饮料,接受可持续性和健康信息对消费者接受度、购买意愿的影响,并使用涉及情绪和感受的术语进行CATA测试。与BCC处理相比,添加面粉降低了饮料的接受率。这些处理在香气和甜味方面受到惩罚。对消费者收到的信息类型没有影响。可能,添加高可可百分比会对产品的接受度产生负面影响,以及使用带有苦味的面粉,因为更容易接受更甜的产品。
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引用次数: 0
Peculiarities of the Organic Wine in Galicia (NW Spain): Sensory Evaluation and Future Considerations 加利西亚(西班牙西北部)有机葡萄酒的特性:感官评价和未来考虑
Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-10-18 DOI: 10.3390/beverages9040089
David Castrillo, Pilar Blanco
Although wine is one of the most studied beverages at a sensory level, there is practically no information on the particularities of organic wines from Galicia. Considering that different regions provide distinctive characteristics to wines, organic and conventional wines from the five Designations of Origin (DO) from Galicia were analyzed at chemical and sensory levels. Sensory analysis showed that organic cultivation favors distinctive quality wines. Organic wines obtained similar scores to conventional wines in the preference tests and, in some cases, they were preferred by the tasters. However, this was not the case with wines produced in climatologically unfavorable seasons. Differences between conventional and organic wines were strongly marked by other factors such as the vintage, the DO, the winemaking techniques, or the yeasts of each winery. The results of this study show that organic wine production favors the differentiation and diversification of Galician wines. This could increase the added value of organic wines and improve their choice in a crowded market.
虽然葡萄酒是在感官水平上研究最多的饮料之一,但实际上没有关于加利西亚有机葡萄酒特殊性的信息。考虑到不同地区的葡萄酒具有不同的特征,我们从化学和感官层面对加利西亚五个原产地(DO)的有机和传统葡萄酒进行了分析。感官分析表明,有机栽培有利于品质独特的葡萄酒。在偏好测试中,有机葡萄酒的得分与传统葡萄酒相似,在某些情况下,它们更受品酒者的青睐。然而,在气候不利的季节生产的葡萄酒并非如此。传统葡萄酒和有机葡萄酒之间的差异明显受到其他因素的影响,如年份、DO、酿酒技术或每个酿酒厂的酵母。本研究的结果表明,有机葡萄酒生产有利于差异化和多样化的加利西亚葡萄酒。这可以增加有机葡萄酒的附加值,并在拥挤的市场中增加他们的选择。
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引用次数: 0
‘Kombucha’-like Beverage of Broccoli By-Products: A New Dietary Source of Bioactive Sulforaphane 西兰花副产品“康普茶”类饮料:生物活性萝卜硫素的新膳食来源
Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-10-12 DOI: 10.3390/beverages9040088
Berta María Cánovas, Cristina García-Viguera, Sonia Medina, Raúl Domínguez-Perles
The objective of this work is the development of a new fermented beverage (‘kombucha’-like), enriched with broccoli by-products as an ingredient, a source of organosulfur compounds, which could be biotransformed into more bioaccessible, bioavailable, and bioactive metabolites. The new beverages have shown variations in the physicochemical (pH, 3.6–6.3; acidity, 0.65–1.39 g/L; °Brix, 4.63–8.20). Moreover, the phytochemical characterization has demonstrated different degrees of metabolization of the glucosinolates, leached during the infusion of the plant material into isothiocyanates (sulforaphane in concentrations up to 31.39 µg/100 mL) and its metabolic derivatives (sulforaphane-N-acetylcysteine in concentrations up to 5.37 µg/100 mL). Therefore, these results demonstrate that the increase in the concentration of the bioactive compounds concentration would provide higher bioavailability and health benefits. This is especially relevant with regard to anti-inflammatory activity. Reporting additional proof of enhanced biological benefits will boost the development of new functional beverages.
这项工作的目的是开发一种新的发酵饮料(类似“康普茶”),富含西兰花副产品作为成分,有机硫化合物的来源,可以被生物转化为更具生物可及性、生物可利用性和生物活性的代谢物。新饮料的物理化学(pH值,3.6-6.3;酸度,0.65-1.39 g/L;°白利糖度,4.63 - -8.20)。此外,植物化学表征表明,硫代葡萄糖苷在异硫氰酸酯(萝卜硫素浓度高达31.39µg/100 mL)及其代谢衍生物(萝卜硫素- n -乙酰半胱氨酸浓度高达5.37µg/100 mL)中浸出时发生了不同程度的代谢。因此,这些结果表明,增加生物活性化合物浓度将提供更高的生物利用度和健康效益。这与抗炎活性尤其相关。报告更多增强生物效益的证据将促进新功能饮料的开发。
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引用次数: 0
Monitoring of Chemical Changes in Coffee Beans during the Roasting Process Using Different Roasting Technologies with Nuclear Magnetic Resonance Spectroscopy 利用核磁共振波谱技术监测不同烘焙工艺下咖啡豆烘焙过程中的化学变化
Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-10-12 DOI: 10.3390/beverages9040087
Vera Gottstein, Katrin Krumbügel, Thomas Kuballa, Steffen Schwarz, Enrico Walch, Pascal Walch, Dirk W. Lachenmeier
The roasting process is an important step in coffee production, leading to important physical and chemical changes that are responsible for the sensory quality of a coffee beverage. Besides the commonly used drum roasters, a newly developed infrared roaster can be used to roast green coffee beans. In this study, 1H nuclear magnetic resonance (NMR) spectroscopy was used to analyze the fat and aqueous extracts of coffee beans roasted to different degrees of roasting using a professional drum roaster, a hot air fluidized bed sample roaster and an infrared roaster. Caffeine-containing and decaffeinated Coffea arabica coffee samples were used to monitor the roasting process in the different roasters. Compared with the drum-roasted coffee sample, the formation and degradation of NMR-detectable components in the coffee sample roasted with the infrared roaster and the hot air roaster were time-dependent. In the decaffeinated coffee sample, compounds such as kahweol, caffeoylquinic acid and trigonelline were found to occur at lower levels. The formation and degradation of the NMR-detectable compounds in the decaffeinated coffee sample also occurred with a time lag or to a lesser extent than in the caffeine-containing coffee sample.
烘焙过程是咖啡生产中的一个重要步骤,它会导致重要的物理和化学变化,从而影响咖啡饮料的感官质量。除了常用的滚筒烘培机外,新开发的红外线烘培机也可用于烘培生咖啡豆。本研究采用1H核磁共振(NMR)波谱技术,分别采用专业滚筒烘焙机、热风流化床样品烘焙机和红外烘焙机对不同烘焙程度的咖啡豆进行脂肪和水萃取物的分析。含咖啡因和不含咖啡因的阿拉比卡咖啡样品被用来监测不同烘焙机的烘焙过程。与鼓烘咖啡样品相比,红外烘焙机和热风烘焙机烘焙的咖啡样品中核磁共振可检测成分的形成和降解具有时间依赖性。在不含咖啡因的咖啡样本中,发现咖啡豆醇、咖啡酰奎宁酸和葫芦巴碱等化合物的含量较低。与含咖啡因的咖啡样品相比,不含咖啡因的咖啡样品中核磁共振可检测化合物的形成和降解也有一定的时间滞后或程度较轻。
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引用次数: 0
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Beverages
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