Pub Date : 2023-08-10DOI: 10.3390/beverages9030066
M. Jackowski, Weronika Czepiela, Laura Hampf, W. Żuczkowski, Tomasz Dymkowski, Anna Trusek
Due to current trends in beer consumption, as well as social aspects, such as the education of society on combining drinking and driving, intensive research and development efforts have been recently focused on producing low-alcohol beers and non-alcoholic beers with a sensory profile appealing to consumers. There are plenty of methods for obtaining such beverages; one of these methods involves utilizing non-conventional yeasts for wort fermentation. In this work, the production of low-alcohol beer using commercially available Saccharomycodes ludwigii and Torulaspora delbrueckii strains were compared. The results showed that Torulaspora delbrueckii achieved the lowest level of attenuation, producing beer with an ethanol concentration of 2.58% vol. Saccharomycodes ludwigii displayed a slightly higher level of attenuation; however, its alcohol concentration was slightly lower than in the case of Torulaspora delbrueckii and reached 2.50% vol. Fully fermented beers produced using Saccharomycodes ludwigii and Torulaspora delbrueckii represented reduced ethanol concentrations by 12% and 15%, respectively, in comparison to Saccharomyces cerevisiae. Nevertheless, in order to produce non-alcoholic beers, arrested fermentation is necessary. In such a case, Saccharomycodes ludwigii reached the highest level of attenuation among non-alcoholic beers.
{"title":"Comparison of Two Commercially Available Strains, Saccharomycodes ludwigii and Torulaspora delbrueckii, for the Production of Low-Alcohol Beer","authors":"M. Jackowski, Weronika Czepiela, Laura Hampf, W. Żuczkowski, Tomasz Dymkowski, Anna Trusek","doi":"10.3390/beverages9030066","DOIUrl":"https://doi.org/10.3390/beverages9030066","url":null,"abstract":"Due to current trends in beer consumption, as well as social aspects, such as the education of society on combining drinking and driving, intensive research and development efforts have been recently focused on producing low-alcohol beers and non-alcoholic beers with a sensory profile appealing to consumers. There are plenty of methods for obtaining such beverages; one of these methods involves utilizing non-conventional yeasts for wort fermentation. In this work, the production of low-alcohol beer using commercially available Saccharomycodes ludwigii and Torulaspora delbrueckii strains were compared. The results showed that Torulaspora delbrueckii achieved the lowest level of attenuation, producing beer with an ethanol concentration of 2.58% vol. Saccharomycodes ludwigii displayed a slightly higher level of attenuation; however, its alcohol concentration was slightly lower than in the case of Torulaspora delbrueckii and reached 2.50% vol. Fully fermented beers produced using Saccharomycodes ludwigii and Torulaspora delbrueckii represented reduced ethanol concentrations by 12% and 15%, respectively, in comparison to Saccharomyces cerevisiae. Nevertheless, in order to produce non-alcoholic beers, arrested fermentation is necessary. In such a case, Saccharomycodes ludwigii reached the highest level of attenuation among non-alcoholic beers.","PeriodicalId":8773,"journal":{"name":"Beverages","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43814631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-03DOI: 10.3390/beverages9030065
M. Marinaki, I. Sampsonidis, A. Nakas, P. Arapitsas, A. Assimopoulou, G. Theodoridis
The most well-known traditional Greek grape marc distillate made from winemaking pomace is called “Tsipouro”. Its production involves various grape pomace cultivars, preparation protocols, and anise-flavoring or not, and it should be a colorless liquid with intense organoleptic properties due to the raw materials used in its production and have a minimum alcoholic strength of 37.5% by volume. This study aimed to characterize the volatilome of tsipouro products by covering as many geographical areas and production styles as possible, as there is a lack of characterization of the aromatic composition of this Greek traditional alcoholic beverage. A Headspace Solid Phase Microextraction Gas Chromatography–Mass Spectrometry (HS-SPME-GC-MS) method was applied in 60 samples, resulting in the identification and semi-quantification of over 90 volatile compounds. The statistical analysis pointed out the metabolites that characterized each traditional product group and underlined the influence of the geographical origin and the production protocol. Aniseed spirits from Northern Greece, Macedonia, Limnos Island, and Thessaly, produced from Muscat pomaces, were found to be richer in terpenes, terpenoids, and flavored compounds, attributing to product aroma and quality; different terpenoids were found to be dominant in Muscat distillates from different regions, showing the importance of geographical origin and production process. In conclusion, the results demonstrated the high aroma variability of the Greek Tsipouro, explained that this diversity is caused mainly by the raw material, and could be helpful in the better protection of the origin of this traditional product and the improvement of its quality.
{"title":"Analysis of the Volatile Organic Compound Fingerprint of Greek Grape Marc Spirits of Various Origins and Traditional Production Styles","authors":"M. Marinaki, I. Sampsonidis, A. Nakas, P. Arapitsas, A. Assimopoulou, G. Theodoridis","doi":"10.3390/beverages9030065","DOIUrl":"https://doi.org/10.3390/beverages9030065","url":null,"abstract":"The most well-known traditional Greek grape marc distillate made from winemaking pomace is called “Tsipouro”. Its production involves various grape pomace cultivars, preparation protocols, and anise-flavoring or not, and it should be a colorless liquid with intense organoleptic properties due to the raw materials used in its production and have a minimum alcoholic strength of 37.5% by volume. This study aimed to characterize the volatilome of tsipouro products by covering as many geographical areas and production styles as possible, as there is a lack of characterization of the aromatic composition of this Greek traditional alcoholic beverage. A Headspace Solid Phase Microextraction Gas Chromatography–Mass Spectrometry (HS-SPME-GC-MS) method was applied in 60 samples, resulting in the identification and semi-quantification of over 90 volatile compounds. The statistical analysis pointed out the metabolites that characterized each traditional product group and underlined the influence of the geographical origin and the production protocol. Aniseed spirits from Northern Greece, Macedonia, Limnos Island, and Thessaly, produced from Muscat pomaces, were found to be richer in terpenes, terpenoids, and flavored compounds, attributing to product aroma and quality; different terpenoids were found to be dominant in Muscat distillates from different regions, showing the importance of geographical origin and production process. In conclusion, the results demonstrated the high aroma variability of the Greek Tsipouro, explained that this diversity is caused mainly by the raw material, and could be helpful in the better protection of the origin of this traditional product and the improvement of its quality.","PeriodicalId":8773,"journal":{"name":"Beverages","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2023-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49658521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-02DOI: 10.3390/beverages9030064
Thomas Jerome Kelly, Christine O'Connor, K. Kilcawley
Whiskey’s complex and diverse flavor stems from a range of reactions that create congeners that are primarily dependent upon the cereal source/mash bill and each stage of the process: malting, mashing, fermentation, distillation, and cask maturation. Therefore, in theory, the congener profile of a whiskey is a summation of its ingredients and the specific parameters of each stage of the manufacturing process. Congener profiles have been used as biomarkers for quality and authentication; however, to date, insufficient information has been published in relation to the extensive profiling of congeners associated with specific whiskey styles/types or the intra-and inter-variability within brands, especially in an Irish context due to the recent rapid expansion of the industry. As the ability to extract and identify congeners has progressed appreciably in recent years due to advances in extraction, chromatographic, and chemometric techniques, it is imperative that research is undertaken to gain a better understanding of the impact of specific congeners not only in relation to quality but also as biomarkers for authentication.
{"title":"Sources of Volatile Aromatic Congeners in Whiskey","authors":"Thomas Jerome Kelly, Christine O'Connor, K. Kilcawley","doi":"10.3390/beverages9030064","DOIUrl":"https://doi.org/10.3390/beverages9030064","url":null,"abstract":"Whiskey’s complex and diverse flavor stems from a range of reactions that create congeners that are primarily dependent upon the cereal source/mash bill and each stage of the process: malting, mashing, fermentation, distillation, and cask maturation. Therefore, in theory, the congener profile of a whiskey is a summation of its ingredients and the specific parameters of each stage of the manufacturing process. Congener profiles have been used as biomarkers for quality and authentication; however, to date, insufficient information has been published in relation to the extensive profiling of congeners associated with specific whiskey styles/types or the intra-and inter-variability within brands, especially in an Irish context due to the recent rapid expansion of the industry. As the ability to extract and identify congeners has progressed appreciably in recent years due to advances in extraction, chromatographic, and chemometric techniques, it is imperative that research is undertaken to gain a better understanding of the impact of specific congeners not only in relation to quality but also as biomarkers for authentication.","PeriodicalId":8773,"journal":{"name":"Beverages","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2023-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47439682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-31DOI: 10.3390/beverages9030063
María Osorio Alises, E. Sánchez-Palomo, M. González-Viñas
The objective of the current research was to study the effect of different alcohol reduction technologies on the chemical aromatic composition of La Mancha Tempranillo rosé wines. Volatile compounds were analysed using Gas Chromatography coupled to Mass Spectrometry (GC-MS), with previous isolation by solid phase extraction (SPE). C6 compounds were the only group of varietal compounds that was modified when the total dealcoholizing process was used. According to their odor descriptor, the volatile compounds were grouped into six odorant series. The total intensity of each aromatic series was calculated by adding the OAVs of the compounds appointed to this series. All wines showed the same sequence, only modified the intensity of the principal aromatic series, mainly in total dealcoholized wines. These studied wines maintain the aromatic typicality independently from ethanol concentration, which highlights the viability of these techniques as an alternative to the traditional winemaking process, which will allow diversifying wine’s actual market.
{"title":"Influence of Different Alcohol Reduction Technologies on the Volatile Composition of La Mancha Tempranillo Rosé Wines","authors":"María Osorio Alises, E. Sánchez-Palomo, M. González-Viñas","doi":"10.3390/beverages9030063","DOIUrl":"https://doi.org/10.3390/beverages9030063","url":null,"abstract":"The objective of the current research was to study the effect of different alcohol reduction technologies on the chemical aromatic composition of La Mancha Tempranillo rosé wines. Volatile compounds were analysed using Gas Chromatography coupled to Mass Spectrometry (GC-MS), with previous isolation by solid phase extraction (SPE). C6 compounds were the only group of varietal compounds that was modified when the total dealcoholizing process was used. According to their odor descriptor, the volatile compounds were grouped into six odorant series. The total intensity of each aromatic series was calculated by adding the OAVs of the compounds appointed to this series. All wines showed the same sequence, only modified the intensity of the principal aromatic series, mainly in total dealcoholized wines. These studied wines maintain the aromatic typicality independently from ethanol concentration, which highlights the viability of these techniques as an alternative to the traditional winemaking process, which will allow diversifying wine’s actual market.","PeriodicalId":8773,"journal":{"name":"Beverages","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2023-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42074979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
To improve the quality of fermented chickpea beverages, a highly nutritious substitute for dairy, the Box-Behnken design and the response surface methodology were used to obtain optimized ultrasonic parameters for producing ultrasound-assisted fermented chickpea beverages. The determining parameters were the lactic acid, reducing sugar content, and the cell viability of the treated product. The most significant parameters obtained were frequency and treatment duration, while power density was relatively insignificant. The optimum fermentation parameters obtained were a treatment start time of 3 h, treatment duration of 80 min, frequency of 27.5 kHz, and power density of 100 W/L with optimum yields of 0.23096 mg/mL, 2.92898 mg/mL, and 0.488189 for reducing sugar, lactic acid, and cell viability index, respectively, with desirability above 0.95. Further analysis of the ultrasound treatment’s effect on the product’s structure showed the ultrasound-assisted fermented chickpea beverage was more structurally stable and homogenous, with even distribution of macromolecules present.
{"title":"Phytochemical and Structural Changes of Chickpea Beverage Prepared Using Ultrasound-Assisted Fermentation with Optimized Ultrasound Parameters Modelled by Response Surface Methodology","authors":"Nana Adwoa Nkuma Johnson, John-Nelson Ekumah, Selorm Yao-Say Solomon Adade, Yanshu Li, Garba Betchem, Eliasu Issaka, Yongkun Ma","doi":"10.3390/beverages9030062","DOIUrl":"https://doi.org/10.3390/beverages9030062","url":null,"abstract":"To improve the quality of fermented chickpea beverages, a highly nutritious substitute for dairy, the Box-Behnken design and the response surface methodology were used to obtain optimized ultrasonic parameters for producing ultrasound-assisted fermented chickpea beverages. The determining parameters were the lactic acid, reducing sugar content, and the cell viability of the treated product. The most significant parameters obtained were frequency and treatment duration, while power density was relatively insignificant. The optimum fermentation parameters obtained were a treatment start time of 3 h, treatment duration of 80 min, frequency of 27.5 kHz, and power density of 100 W/L with optimum yields of 0.23096 mg/mL, 2.92898 mg/mL, and 0.488189 for reducing sugar, lactic acid, and cell viability index, respectively, with desirability above 0.95. Further analysis of the ultrasound treatment’s effect on the product’s structure showed the ultrasound-assisted fermented chickpea beverage was more structurally stable and homogenous, with even distribution of macromolecules present.","PeriodicalId":8773,"journal":{"name":"Beverages","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2023-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46222303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-24DOI: 10.3390/beverages9030061
Maria Góis, Patrícia Batista, Magnólia Araújo, P. Oliveira-Silva
Probiotic products have been the focus of research for several years due to the potential of their biological properties to impact mental health, mood, and cognitive functions. Kombucha is a probiotic drink that has been reported to be beneficial for mental health, particularly at the level of emotion regulation. This study aims to understand the perception of the Portuguese and Brazilian populations regarding the consumption of probiotics and Kombucha, as well as to understand these consumers’ perceptions related to the impact on emotion regulation (and the impact of this consumption on emotion regulation). The research was conducted through an online questionnaire and had a total sample of 256 participants. The results show that there are no statistically significant differences between the consumption of probiotics and Kombucha when comparing the Portuguese and Brazilian samples. Additionally, this study reveals a significant association between probiotic consumption patterns in both the Portuguese and Brazilian samples. However, no statistically significant relationship was found between the consumption of probiotics and Kombucha and emotion regulation. This study intends to contribute to the increase in knowledge about the perception of probiotics and Kombucha consumption in relation to emotion regulation, and to draw attention to the importance of this topic in the community (society, academia, and industry).
{"title":"Perceptions of Probiotics and Kombucha Consumption in Relation to Emotion Regulation: An Exploratory Study Comparing Portugal and Brazil","authors":"Maria Góis, Patrícia Batista, Magnólia Araújo, P. Oliveira-Silva","doi":"10.3390/beverages9030061","DOIUrl":"https://doi.org/10.3390/beverages9030061","url":null,"abstract":"Probiotic products have been the focus of research for several years due to the potential of their biological properties to impact mental health, mood, and cognitive functions. Kombucha is a probiotic drink that has been reported to be beneficial for mental health, particularly at the level of emotion regulation. This study aims to understand the perception of the Portuguese and Brazilian populations regarding the consumption of probiotics and Kombucha, as well as to understand these consumers’ perceptions related to the impact on emotion regulation (and the impact of this consumption on emotion regulation). The research was conducted through an online questionnaire and had a total sample of 256 participants. The results show that there are no statistically significant differences between the consumption of probiotics and Kombucha when comparing the Portuguese and Brazilian samples. Additionally, this study reveals a significant association between probiotic consumption patterns in both the Portuguese and Brazilian samples. However, no statistically significant relationship was found between the consumption of probiotics and Kombucha and emotion regulation. This study intends to contribute to the increase in knowledge about the perception of probiotics and Kombucha consumption in relation to emotion regulation, and to draw attention to the importance of this topic in the community (society, academia, and industry).","PeriodicalId":8773,"journal":{"name":"Beverages","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2023-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45902445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-18DOI: 10.3390/beverages9030060
Veronica D’Eusanio, Francesco Genua, A. Marchetti, L. Morelli, L. Tassi
This study aimed to assess the metal content of tannic extracts obtained from grapevine canes, to evaluate their suitability as wood shavings for direct infusion during the aging process of alcoholic beverages or vinegars. Traditional barrel aging is a slow and costly process that can be enhanced through direct infusion of wood chips. Our investigation focused on the pruning materials of two widely cultivated Lambrusco cultivars in the Modena (Italy) area, Ancellotta and Salamino. The grapevine chips underwent preliminary heat treatments at temperatures ranging from 120 °C to 260 °C. Tannic extracts were obtained by ethanol maceration at 80 °C for 2 h. The metal composition was determined using inductively coupled plasma optical emission spectroscopy. Generally, the metal content increased with the roasting temperature of the chips. Two exceptions were noted in the Salamino extracts, where the concentrations of Bi and Ni decreased as the roasting temperature increased. The levels of heavy metal, such as Pb and Cd, were low, posing no toxicity concerns for using infused grapevine chips during the beverage aging process. The Ni concentration slightly exceeded the limits imposed by certain regulations. Its content is likely derived from the type of soil in which the plant has grown.
{"title":"Exploring the Mineral Composition of Grapevine Canes for Wood Chip Applications in Alcoholic Beverage Production to Enhance Viticulture Sustainability","authors":"Veronica D’Eusanio, Francesco Genua, A. Marchetti, L. Morelli, L. Tassi","doi":"10.3390/beverages9030060","DOIUrl":"https://doi.org/10.3390/beverages9030060","url":null,"abstract":"This study aimed to assess the metal content of tannic extracts obtained from grapevine canes, to evaluate their suitability as wood shavings for direct infusion during the aging process of alcoholic beverages or vinegars. Traditional barrel aging is a slow and costly process that can be enhanced through direct infusion of wood chips. Our investigation focused on the pruning materials of two widely cultivated Lambrusco cultivars in the Modena (Italy) area, Ancellotta and Salamino. The grapevine chips underwent preliminary heat treatments at temperatures ranging from 120 °C to 260 °C. Tannic extracts were obtained by ethanol maceration at 80 °C for 2 h. The metal composition was determined using inductively coupled plasma optical emission spectroscopy. Generally, the metal content increased with the roasting temperature of the chips. Two exceptions were noted in the Salamino extracts, where the concentrations of Bi and Ni decreased as the roasting temperature increased. The levels of heavy metal, such as Pb and Cd, were low, posing no toxicity concerns for using infused grapevine chips during the beverage aging process. The Ni concentration slightly exceeded the limits imposed by certain regulations. Its content is likely derived from the type of soil in which the plant has grown.","PeriodicalId":8773,"journal":{"name":"Beverages","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2023-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44435583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-11DOI: 10.3390/beverages9030059
Keisha Harrison, Roxana Navarro, Kristen Jensen, William F Cayler, Tom Nielsen, Chris Curtin
Kombucha is an acidic non-alcoholic fermented tea beverage that has surged in popularity over the past decade. A key driver of this popularity is the perception that kombucha contains microbes that contribute to gut health, a perception that is reinforced by package claims such as “live” and “probiotic”. We surveyed retail-available non-alcoholic (soft) and alcoholic (hard) kombucha and selected 39 products, of which 74.4% included at least one of these claims. Based upon Nuclear Magnetic Resonance (NMR) evaluation of the chemical composition, approximately one-third of soft kombucha products were re-categorized as “soft-aberrant” on the basis of their alcohol by volume (ABV) exceeding 0.5%. Across all three categories, the majority (82.1%) of products were “live”, containing culturable yeast and/or bacteria. However, there were no significant differences in median maximum colony-forming unit (CFU) counts between these categories, nor was there a difference according to package claims. Interestingly, only 6.3% of soft and 10% of soft-aberrant kombucha products exceeded 106 CFU/mL, the threshold that would deliver at least one billion cells in one package. None of the sampled hard kombucha exceeded this threshold. Metabarcoding analyses of microbial communities revealed differences in composition between categories and according to package claims. Notably, the subset of products with “probiotic” claims that listed Bacillus coagulans as an ingredient were enriched in read counts for Bacillus.
{"title":"Live, Probiotic, or Neither? Microbial Composition of Retail-Available Kombucha and “Hard” Kombucha in the Pacific Northwest of the United States","authors":"Keisha Harrison, Roxana Navarro, Kristen Jensen, William F Cayler, Tom Nielsen, Chris Curtin","doi":"10.3390/beverages9030059","DOIUrl":"https://doi.org/10.3390/beverages9030059","url":null,"abstract":"Kombucha is an acidic non-alcoholic fermented tea beverage that has surged in popularity over the past decade. A key driver of this popularity is the perception that kombucha contains microbes that contribute to gut health, a perception that is reinforced by package claims such as “live” and “probiotic”. We surveyed retail-available non-alcoholic (soft) and alcoholic (hard) kombucha and selected 39 products, of which 74.4% included at least one of these claims. Based upon Nuclear Magnetic Resonance (NMR) evaluation of the chemical composition, approximately one-third of soft kombucha products were re-categorized as “soft-aberrant” on the basis of their alcohol by volume (ABV) exceeding 0.5%. Across all three categories, the majority (82.1%) of products were “live”, containing culturable yeast and/or bacteria. However, there were no significant differences in median maximum colony-forming unit (CFU) counts between these categories, nor was there a difference according to package claims. Interestingly, only 6.3% of soft and 10% of soft-aberrant kombucha products exceeded 106 CFU/mL, the threshold that would deliver at least one billion cells in one package. None of the sampled hard kombucha exceeded this threshold. Metabarcoding analyses of microbial communities revealed differences in composition between categories and according to package claims. Notably, the subset of products with “probiotic” claims that listed Bacillus coagulans as an ingredient were enriched in read counts for Bacillus.","PeriodicalId":8773,"journal":{"name":"Beverages","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2023-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45392688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-10DOI: 10.3390/beverages9030058
Intan Dewi Larasati, Nurul Mutmainah Diah Oktaviani, H. Lioe, T. Estiasih, M. Palma, W. Setyaningsih
Edible flowers, including roselle, contain a varied composition of phenolic compounds that may inhibit the oxidative stress mechanism. Roselle-based tisane with appealing sensory properties is commonly consumed worldwide. However, the conventional hot-brew method may ruin the stability of thermolabile phenolic compounds during the tisane preparation. Hence, this study aimed to develop a new alternative brewing method linked with the new cold-brew method, which involves a lower temperature and applying ultrasound to maximize the extraction of phenolic compounds and to avoid degradation during the tisane preparation. The brewing factors, including particle size (10, 20, 30 mesh), temperature (4, 15, 26 °C), time (10, 20, 30 min), and ultrasound amplitude (20, 60, 100% of the maximum amplitude) have been optimized simultaneously using Box–Behnken design in conjunction with response surface methodology. Seven major phenolic compounds were identified by HPLC-DAD and classified into hydroxycinnamic acid derivatives (HCA) and flavonoids. The optimum extraction condition to reach the highest level of the studied phenolic compounds was set to brew roselle with particle size of 30 ± 3.25 mesh at 26 ± 1.32 °C for 18 ± 2.00 min applying 78 ± 5.64% ultrasound amplitude. This method successfully extracted almost all HCA and flavonoid during the first cycle with less than 10% CV and provided higher antioxidant activity in terms of DPPH (IC50 9.77 ± 0.01 µg mL−1), ABTS (IC50 8.05 ± 0.02 µg mL−1), and FRAP (IC50 10.34 ± 0.03 µg mL−1) than the roselle tisane prepared using the conventional method. Additionally, the resulting cold-brew product was stable for up to five days of storage.
食用花卉，包括玫瑰，含有多种酚类化合物的组成，可能抑制氧化应激机制。玫瑰花组织具有吸引人的感官特性，在世界范围内普遍使用。然而，传统的热冲泡方法可能会破坏组织制备过程中耐热性酚类化合物的稳定性。因此，本研究旨在开发一种新的替代酿造方法，与新的冷酿造方法相结合，通过更低的温度和超声波来最大限度地提取酚类化合物，并避免在组织制备过程中降解。利用Box-Behnken设计与响应面法同时优化了酿造因素，包括粒度(10、20、30目)、温度(4、15、26°C)、时间(10、20、30分钟)和超声振幅(最大振幅的20%、60%、100%)。HPLC-DAD鉴定了7种主要酚类化合物，并将其分为羟基肉桂酸衍生物(HCA)和黄酮类化合物。最佳提取条件为:在26±1.32°C条件下，超声振幅为78±5.64%，提取时间为18±2.00 min，提取粒度为30±3.25目。该方法在第一个周期内以低于10%的CV成功提取了几乎所有的HCA和黄酮类化合物，并且在DPPH (IC50为9.77±0.01µg mL - 1)、ABTS (IC50为8.05±0.02µg mL - 1)和FRAP (IC50为10.34±0.03µg mL - 1)方面具有比常规方法更高的抗氧化活性。此外，由此产生的冷冲泡产品在长达五天的储存中是稳定的。
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Pub Date : 2023-07-03DOI: 10.3390/beverages9030056
V. Athanasiadis, Theodoros G. Chatzimitakos, Dimitrios Kalompatsios, Martha Mantiniotou, Eleni Bozinou, S. Lalas
The study focused on the examination of eight commercial hypertonic, isotonic, and hypotonic beverage samples that were packaged in aluminum cans or polyethylene terephthalate (PET) bottles. The pH value, caffeine content, and concentration of thirty elements were determined. Caffeine quantification was performed by a high-performance liquid chromatography method. Element determination was conducted by using inductively coupled plasma–optical emission spectrometry. The results showed that pH values were much more acidic in all samples, ranging from 2.68 to 3.97. Caffeine concentrations ranged from 0.02–38.93 mg/100 mL between the samples and were found to be below the acceptable daily level (~400 mg) established by the relevant food regulatory authority. The measurable quantity of caffeine (~10 mg/100 mL) was found in two functional beverage samples where caffeine was labeled as an ingredient. Element concentration varied with statistically significant differences (p < 0.05) among all samples. Elements such as arsenic, cadmium, chromium, and lead were measured below the corresponding permissible levels set by the European Commission; however, daily consumption of functional beverages should be examined to deteriorate several health risks. Aluminum was quantified above the parametric value by 21 to 117% in the canned samples, whereas antimony was only measured in PET-bottled samples at approximately 3 μg/L. Multivariate methods were applied to investigate any possible correlation between the samples and the examined parameters. Strong positive correlations with statistically significant differences (R > 0.9, p < 0.05) were observed between zinc and copper and between calcium and silver. Finally, similarities and differences between the samples and the examined parameters resulted in satisfactory discrimination of them regarding not only their caffeine content but also their tonicity. Excessive consumption of functional beverages could represent a major public health issue due to elevated amounts of caffeine and elements. Consequently, the results of this research could facilitate the formulation of stricter standards in beverage consumption by revealing potential health hazards to consumers.
{"title":"Determination of Caffeine and Elements in Hypertonic, Isotonic, and Hypotonic Beverages","authors":"V. Athanasiadis, Theodoros G. Chatzimitakos, Dimitrios Kalompatsios, Martha Mantiniotou, Eleni Bozinou, S. Lalas","doi":"10.3390/beverages9030056","DOIUrl":"https://doi.org/10.3390/beverages9030056","url":null,"abstract":"The study focused on the examination of eight commercial hypertonic, isotonic, and hypotonic beverage samples that were packaged in aluminum cans or polyethylene terephthalate (PET) bottles. The pH value, caffeine content, and concentration of thirty elements were determined. Caffeine quantification was performed by a high-performance liquid chromatography method. Element determination was conducted by using inductively coupled plasma–optical emission spectrometry. The results showed that pH values were much more acidic in all samples, ranging from 2.68 to 3.97. Caffeine concentrations ranged from 0.02–38.93 mg/100 mL between the samples and were found to be below the acceptable daily level (~400 mg) established by the relevant food regulatory authority. The measurable quantity of caffeine (~10 mg/100 mL) was found in two functional beverage samples where caffeine was labeled as an ingredient. Element concentration varied with statistically significant differences (p < 0.05) among all samples. Elements such as arsenic, cadmium, chromium, and lead were measured below the corresponding permissible levels set by the European Commission; however, daily consumption of functional beverages should be examined to deteriorate several health risks. Aluminum was quantified above the parametric value by 21 to 117% in the canned samples, whereas antimony was only measured in PET-bottled samples at approximately 3 μg/L. Multivariate methods were applied to investigate any possible correlation between the samples and the examined parameters. Strong positive correlations with statistically significant differences (R > 0.9, p < 0.05) were observed between zinc and copper and between calcium and silver. Finally, similarities and differences between the samples and the examined parameters resulted in satisfactory discrimination of them regarding not only their caffeine content but also their tonicity. Excessive consumption of functional beverages could represent a major public health issue due to elevated amounts of caffeine and elements. Consequently, the results of this research could facilitate the formulation of stricter standards in beverage consumption by revealing potential health hazards to consumers.","PeriodicalId":8773,"journal":{"name":"Beverages","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2023-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47546634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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