首页 > 最新文献

Biological Trace Element Research最新文献

英文 中文
GPR44-Dependent Regulation of the Selenoproteome by eIF4a3 in Murine Acute Myeloid Leukemia-Initiating Stem Cells. eIF4a3在小鼠急性髓系白血病起始干细胞中依赖gpr44的硒蛋白组调控
IF 3.6 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-02-01 Epub Date: 2025-06-27 DOI: 10.1007/s12011-025-04724-9
Deborpita Sarkar, Fenghua Qian, Robert F Paulson, K Sandeep Prabhu

Acute myeloid leukemia (AML) remains an aggressive hematologic malignancy, with leukemia-initiating stem cells (LICs) playing a critical role in disease progression and therapeutic resistance. In this study, we investigated the role of GPR44, a G-protein coupled receptor of arachidonic acid-derived prostaglandin D2 (PGD2) and its cyclopentenone prostaglandins (CyPGs) metabolites, Δ12-PGJ2 and 15d-PGJ2, in regulating selenium metabolism and selenoprotein expression in AML LICs. Transplantation of Gpr44-/- LICs into donor mice led to aggressive leukemogenesis. Transcriptomic and proteomic analyses revealed that GPR44 deletion significantly altered the selenoproteome, with downregulation of Txnrd1, Txnrd3, Selenop, and Gpx2, while upregulating Gpx3, Gpx4, Selenoo, and Msrb1. These findings suggest that GPR44 influences redox homeostasis and leukemic cell survival by modulating selenium utilization. Notably, increased expression of eIF4a3 in Gpr44-/- LICs suggested a potential mechanism for selective selenoprotein repression through SECIS-binding protein 2 (SBP2) inhibition. Additionally, upregulation of SBP2 and selenophosphate synthetase 2 (SPS2) indicated an adaptive response to maintain selenium incorporation. Given the role of selenium in redox balance, metabolism, and immune function, targeting selenium metabolism in GPR44-expressing AML may offer a novel therapeutic approach. Our findings reveal a previously unrecognized link between GPR44 signaling, selenium metabolism, and leukemia progression, warranting further studies to explore selenoprotein-targeting strategies for AML treatment.

急性髓性白血病(AML)仍然是一种侵袭性血液系统恶性肿瘤,白血病起始干细胞(lic)在疾病进展和治疗耐药性中起着关键作用。在这项研究中,我们研究了花生四烯酸衍生前列腺素D2 (PGD2)及其环戊烯酮前列腺素(CyPGs)代谢物Δ12-PGJ2和15d-PGJ2的g蛋白偶联受体GPR44在AML LICs中调节硒代谢和硒蛋白表达的作用。将Gpr44-/- LICs移植给供体小鼠可导致侵袭性白血病的发生。转录组学和蛋白质组学分析显示,GPR44缺失显著改变硒蛋白组,下调Txnrd1、Txnrd3、Selenop和Gpx2,上调Gpx3、Gpx4、Selenoo和Msrb1。这些发现表明GPR44通过调节硒的利用来影响氧化还原稳态和白血病细胞的存活。值得注意的是,Gpr44-/- lic中eIF4a3的表达增加,表明通过抑制secis结合蛋白2 (SBP2)选择性抑制硒蛋白的潜在机制。此外,SBP2和硒磷酸合成酶2 (SPS2)的上调表明了维持硒掺入的适应性反应。考虑到硒在氧化还原平衡、代谢和免疫功能中的作用,靶向表达gpr44的AML中的硒代谢可能提供一种新的治疗方法。我们的研究结果揭示了GPR44信号、硒代谢和白血病进展之间先前未被认识到的联系,值得进一步研究探索硒蛋白靶向治疗AML的策略。
{"title":"GPR44-Dependent Regulation of the Selenoproteome by eIF4a3 in Murine Acute Myeloid Leukemia-Initiating Stem Cells.","authors":"Deborpita Sarkar, Fenghua Qian, Robert F Paulson, K Sandeep Prabhu","doi":"10.1007/s12011-025-04724-9","DOIUrl":"10.1007/s12011-025-04724-9","url":null,"abstract":"<p><p>Acute myeloid leukemia (AML) remains an aggressive hematologic malignancy, with leukemia-initiating stem cells (LICs) playing a critical role in disease progression and therapeutic resistance. In this study, we investigated the role of GPR44, a G-protein coupled receptor of arachidonic acid-derived prostaglandin D<sub>2</sub> (PGD<sub>2</sub>) and its cyclopentenone prostaglandins (CyPGs) metabolites, Δ<sup>12</sup>-PGJ<sub>2</sub> and 15d-PGJ<sub>2</sub>, in regulating selenium metabolism and selenoprotein expression in AML LICs. Transplantation of Gpr44<sup>-/-</sup> LICs into donor mice led to aggressive leukemogenesis. Transcriptomic and proteomic analyses revealed that GPR44 deletion significantly altered the selenoproteome, with downregulation of Txnrd1, Txnrd3, Selenop, and Gpx2, while upregulating Gpx3, Gpx4, Selenoo, and Msrb1. These findings suggest that GPR44 influences redox homeostasis and leukemic cell survival by modulating selenium utilization. Notably, increased expression of eIF4a3 in Gpr44<sup>-/-</sup> LICs suggested a potential mechanism for selective selenoprotein repression through SECIS-binding protein 2 (SBP2) inhibition. Additionally, upregulation of SBP2 and selenophosphate synthetase 2 (SPS2) indicated an adaptive response to maintain selenium incorporation. Given the role of selenium in redox balance, metabolism, and immune function, targeting selenium metabolism in GPR44-expressing AML may offer a novel therapeutic approach. Our findings reveal a previously unrecognized link between GPR44 signaling, selenium metabolism, and leukemia progression, warranting further studies to explore selenoprotein-targeting strategies for AML treatment.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"1001-1007"},"PeriodicalIF":3.6,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12847157/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144504802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Gene Dynamics in Maternal Nutrition: The Role of Chromium in Mitigating Offspring Obesity. 母亲营养中的基因动力学:铬在减轻后代肥胖中的作用。
IF 3.6 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-02-01 Epub Date: 2025-06-19 DOI: 10.1007/s12011-025-04711-0
Xiaofeng Li, Tao Wang, Wei Wang, Zhongyuan Wang, Hongyan Wang, Kewei Fan, Bing Yang

This study investigates the potential mechanisms by which maternal chromium (Cr) supplementation mitigates offspring obesity through identification of key regulatory genes. Sixteen pregnant C57BL/6 mice were randomly allocated to either control (CON) or chromium-supplemented (CR) dietary groups, with nutritional interventions administered throughout gestation and lactation. Post-weaning, all offspring received CR diets until 32 weeks of age. Comparative analysis revealed significant reductions in adipose tissue mass and 136 differentially expressed genes (DEGs) in CR offspring adipose tissue, identified through GEO2R analysis. Functional enrichment analyses revealed significant involvement of these DEGs in critical metabolic pathways: lipid metabolism regulation (PPAR signaling), steroid hormone biosynthesis, cholesterol homeostasis, and fatty acid oxidation processes. Protein-protein interaction network analysis identified 14 hub genes central to adipocyte regulation, including ACSL1, DGAT1, ADIPOQ, PPARD, PPARG, SREBF1, APOC2, and FABP1, which collectively demonstrate synergistic effects in suppressing lipid accumulation. These findings elucidate molecular mechanisms underlying chromium-mediated protection against metabolic disorders and propose novel therapeutic targets for intergenerational obesity prevention. The identified gene network provides a framework for developing maternal nutritional strategies and pharmacological interventions targeting epigenetic regulation of lipid metabolism.

本研究通过鉴定关键调控基因,探讨母体铬(Cr)补充减轻后代肥胖的潜在机制。将16只妊娠C57BL/6小鼠随机分为对照组(CON)和加铬组(CR),在妊娠和哺乳期给予营养干预。断奶后,所有幼崽均饲喂CR日粮,直至32周龄。对比分析显示,通过GEO2R分析发现,CR后代脂肪组织中脂肪组织质量和136个差异表达基因(DEGs)显著减少。功能富集分析显示,这些deg在关键的代谢途径中有重要的参与:脂质代谢调节(PPAR信号传导)、类固醇激素生物合成、胆固醇稳态和脂肪酸氧化过程。蛋白-蛋白相互作用网络分析鉴定出14个脂肪细胞调控中心基因,包括ACSL1、DGAT1、ADIPOQ、PPARD、PPARG、SREBF1、APOC2和FABP1,它们共同显示出抑制脂质积累的协同作用。这些发现阐明了铬介导的代谢紊乱保护的分子机制,并提出了代际肥胖预防的新治疗靶点。鉴定的基因网络为制定针对脂质代谢表观遗传调控的孕产妇营养策略和药物干预提供了框架。
{"title":"Gene Dynamics in Maternal Nutrition: The Role of Chromium in Mitigating Offspring Obesity.","authors":"Xiaofeng Li, Tao Wang, Wei Wang, Zhongyuan Wang, Hongyan Wang, Kewei Fan, Bing Yang","doi":"10.1007/s12011-025-04711-0","DOIUrl":"10.1007/s12011-025-04711-0","url":null,"abstract":"<p><p>This study investigates the potential mechanisms by which maternal chromium (Cr) supplementation mitigates offspring obesity through identification of key regulatory genes. Sixteen pregnant C57BL/6 mice were randomly allocated to either control (CON) or chromium-supplemented (CR) dietary groups, with nutritional interventions administered throughout gestation and lactation. Post-weaning, all offspring received CR diets until 32 weeks of age. Comparative analysis revealed significant reductions in adipose tissue mass and 136 differentially expressed genes (DEGs) in CR offspring adipose tissue, identified through GEO2R analysis. Functional enrichment analyses revealed significant involvement of these DEGs in critical metabolic pathways: lipid metabolism regulation (PPAR signaling), steroid hormone biosynthesis, cholesterol homeostasis, and fatty acid oxidation processes. Protein-protein interaction network analysis identified 14 hub genes central to adipocyte regulation, including ACSL1, DGAT1, ADIPOQ, PPARD, PPARG, SREBF1, APOC2, and FABP1, which collectively demonstrate synergistic effects in suppressing lipid accumulation. These findings elucidate molecular mechanisms underlying chromium-mediated protection against metabolic disorders and propose novel therapeutic targets for intergenerational obesity prevention. The identified gene network provides a framework for developing maternal nutritional strategies and pharmacological interventions targeting epigenetic regulation of lipid metabolism.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"820-835"},"PeriodicalIF":3.6,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bioaccessibility and Health Risk Assessment of Arsenic in Realgar-Containing Traditional Chinese Medicines. 含雄黄中药中砷的生物可及性及健康风险评价
IF 3.6 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-02-01 Epub Date: 2025-06-12 DOI: 10.1007/s12011-025-04700-3
Jianchao Li, Nuo Deng, Fei Wang, Huilin Zhu, Yongming Li, Xiao Wu

As a traditional Chinese medicine (TCM) composed of As2S2, realgar has been widely used for treating various diseases. However, its safety has raised significant concerns due to the well-documented toxicity of arsenic. The Chinese Pharmacopoeia has imposed strict controls on the dosage of realgar, but the bioaccessibility and health risks associated with arsenic in realgar-containing TCMs remain poorly understood. This study developed a hydride generation-atomic fluorescence spectrometry (HG-AFS) method to determine the total arsenic and bioaccessible arsenic contents in 20 realgar-containing TCMs through wet digestion and simulated gastrointestinal fluid extraction. Health risk assessments were evaluated from the bioaccessible arsenic content using single pollution index (Pi), maximum daily intake (MDI), estimated daily intake (EDI), hazard quotient (HQ), and carcinogenic risk (CR). Additionally, the particle size of realgar in representative preparations was analyzed using a light microscope. The tested realgar-containing TCMs exhibited significant variations in the contents of total arsenic and bioaccessible arsenic. Bioaccessible arsenic is a more reasonable indicator for the safety evaluation of realgar-containing TCMs. The daily dosage of realgar in its-containing TCMs exceeded the limit standard prescribed in the Chinese Pharmacopoeia. The evaluation of the health risk index suggested that arsenic in realgar-containing TCMs exceeded the corresponding regulatory thresholds. Furthermore, realgar crystals in preparations were not uniform and exhibited noticeable differences under the microscope. The current quality standards in the Chinese Pharmacopoeia are insufficient to ensure the safety of realgar-containing TCMs, which urgently need to be revised. The study provided scientific evidence for the quality control and clinical safety application of realgar-containing TCMs.

雄黄作为一种由As2S2组成的中药,被广泛用于治疗各种疾病。然而,由于砷的毒性有充分的证据,它的安全性引起了极大的关注。中国药典对雄黄的剂量进行了严格的控制,但对含雄黄中药中砷的生物可及性和健康风险仍知之甚少。本研究建立了氢化物发生-原子荧光光谱法(HG-AFS)测定20种含雄黄中药中总砷和生物可及砷含量的方法。采用单污染指数(Pi)、最大日摄入量(MDI)、估计日摄入量(EDI)、危害商(HQ)和致癌风险(CR)对生物可及性砷含量进行健康风险评价。另外,用光镜对代表性制剂中雄黄的粒径进行了分析。含雄黄中药的总砷含量和生物可及性砷含量存在显著差异。生物可及性砷是评价含雄黄中药安全性较为合理的指标。含雄黄中药的日用量超过《中国药典》规定的限量标准。健康风险指数评价表明,含雄黄中药中砷含量超过相应的监管阈值。此外,各制剂中的雄黄结晶并不均匀,在显微镜下表现出明显的差异。现行《中国药典》质量标准不足以保证含雄黄中药的安全性,亟待修订。本研究为含雄黄中药的质量控制和临床安全应用提供了科学依据。
{"title":"Bioaccessibility and Health Risk Assessment of Arsenic in Realgar-Containing Traditional Chinese Medicines.","authors":"Jianchao Li, Nuo Deng, Fei Wang, Huilin Zhu, Yongming Li, Xiao Wu","doi":"10.1007/s12011-025-04700-3","DOIUrl":"10.1007/s12011-025-04700-3","url":null,"abstract":"<p><p>As a traditional Chinese medicine (TCM) composed of As<sub>2</sub>S<sub>2</sub>, realgar has been widely used for treating various diseases. However, its safety has raised significant concerns due to the well-documented toxicity of arsenic. The Chinese Pharmacopoeia has imposed strict controls on the dosage of realgar, but the bioaccessibility and health risks associated with arsenic in realgar-containing TCMs remain poorly understood. This study developed a hydride generation-atomic fluorescence spectrometry (HG-AFS) method to determine the total arsenic and bioaccessible arsenic contents in 20 realgar-containing TCMs through wet digestion and simulated gastrointestinal fluid extraction. Health risk assessments were evaluated from the bioaccessible arsenic content using single pollution index (Pi), maximum daily intake (MDI), estimated daily intake (EDI), hazard quotient (HQ), and carcinogenic risk (CR). Additionally, the particle size of realgar in representative preparations was analyzed using a light microscope. The tested realgar-containing TCMs exhibited significant variations in the contents of total arsenic and bioaccessible arsenic. Bioaccessible arsenic is a more reasonable indicator for the safety evaluation of realgar-containing TCMs. The daily dosage of realgar in its-containing TCMs exceeded the limit standard prescribed in the Chinese Pharmacopoeia. The evaluation of the health risk index suggested that arsenic in realgar-containing TCMs exceeded the corresponding regulatory thresholds. Furthermore, realgar crystals in preparations were not uniform and exhibited noticeable differences under the microscope. The current quality standards in the Chinese Pharmacopoeia are insufficient to ensure the safety of realgar-containing TCMs, which urgently need to be revised. The study provided scientific evidence for the quality control and clinical safety application of realgar-containing TCMs.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"624-638"},"PeriodicalIF":3.6,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144274059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Assessment of Trace and Macro Elements in Medicinal Plants: Implications for Safety and Phytoremediation. 药用植物中微量和宏量元素的评估:对安全性和植物修复的意义。
IF 3.6 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-02-01 Epub Date: 2025-07-02 DOI: 10.1007/s12011-025-04714-x
Ziba Hurseresht, Mohsen Jalali

The utilization of medicinal plants in Iran and other parts of the world is increasing, and there is a need to study the levels of macro and trace elements, including toxic elements, in medicinal plants. This study analyzed trace and macro elements in 42 medicinal plant samples from a medicinal plant garden in Hamadan city, western Iran. The average trace element contents (mg kg-1 dry weight) in the aerial parts were 1.29 mg kg-1 for Cd, 10.5 mg kg-1 for Cu, 75.5 mg kg-1 for Fe, 41.3 mg kg-1 for Mn, 3.22 mg kg-1 for Ni, and 19.7 mg kg-1 for Zn. Iron was highest in Origanum majorana (96.7 mg kg-1), while Cd was lowest in Inula helenium (0.85 mg kg-1). For macro elements, the highest values were Ca in Urtica dioica (27,800 mg kg-1), K in Echium amoenum (34,600 mg kg-1), Mg in Verbascum thapsus (9080 mg kg-1), and Na in Borago officinalis (10,500 mg kg-1). Relative to adult RDAs, Marrubium vulgare is a key source of Ca (64%) and K (22%), Achillea millefolium provides Mg (98%), Fe (54%), and Mn (97%), and Matricaria chamomilla contributes Cu (86%). The bioaccumulation factor (BCF) averaged 0.68 for Cd, 0.27 for Cu, 0.005 for Fe, 0.26 for Mn, 0.05 for Ni, and 0.44 for Zn, with the highest BCF for Cd (0.92 in Urtica dioica) and lowest for Fe (0.0031 in Marrubium vulgare). The Alcea kurdica, Plantago major, and Urtica dioica may be considered in phytoremediation due to the higher Cd and Ni accumulation. Among studied trace elements, Cd had the highest hazard quotient. This study highlights both the nutritional benefits and safety hazards linked to medicinal plants and proposes some medicinal plants for possible phytoremediation of contaminated soils.

伊朗和世界其他地区对药用植物的利用正在增加,有必要研究药用植物中宏量元素和微量元素,包括有毒元素的水平。本研究分析了来自伊朗西部哈马丹市一个药用植物园的42种药用植物样品中的微量元素和宏量元素。地上部分的平均微量元素含量(干重)分别为Cd 1.29 mg kg-1、Cu 10.5 mg kg-1、Fe 75.5 mg kg-1、Mn 41.3 mg kg-1、Ni 3.22 mg kg-1、Zn 19.7 mg kg-1。铁含量最高的是牛头花(96.7 mg kg-1),而镉含量最低的是牛头花(0.85 mg kg-1)。微量元素含量最高的是杜鹃花Ca (27800 mg kg-1)、石竹K (34600 mg kg-1)、马齿苋mg (9080 mg kg-1)和蒲公英Na (10500 mg kg-1)。相对于成虫的日摄食量,黄丸是主要的钙(64%)和钾(22%)来源,千叶阿喀那提供Mg(98%)、Fe(54%)和Mn(97%),洋甘菊提供Cu(86%)。Cd、Cu、Fe、Mn、Ni、Zn的生物积累因子(BCF)平均为0.68、0.27、0.005、0.26、0.05和0.44,其中Cd的BCF最高,为白花苜蓿(Urtica dioica) 0.92, Fe最低,为普通Marrubium vulgare) 0.0031。由于苜蓿、车前草和荨麻具有较高的Cd和Ni积累,可以考虑用于植物修复。在所研究的微量元素中,镉的危害系数最高。本研究强调了药用植物的营养价值和安全危害,并提出了一些可能用于污染土壤植物修复的药用植物。
{"title":"Assessment of Trace and Macro Elements in Medicinal Plants: Implications for Safety and Phytoremediation.","authors":"Ziba Hurseresht, Mohsen Jalali","doi":"10.1007/s12011-025-04714-x","DOIUrl":"10.1007/s12011-025-04714-x","url":null,"abstract":"<p><p>The utilization of medicinal plants in Iran and other parts of the world is increasing, and there is a need to study the levels of macro and trace elements, including toxic elements, in medicinal plants. This study analyzed trace and macro elements in 42 medicinal plant samples from a medicinal plant garden in Hamadan city, western Iran. The average trace element contents (mg kg<sup>-1</sup> dry weight) in the aerial parts were 1.29 mg kg<sup>-1</sup> for Cd, 10.5 mg kg<sup>-1</sup> for Cu, 75.5 mg kg<sup>-1</sup> for Fe, 41.3 mg kg<sup>-1</sup> for Mn, 3.22 mg kg<sup>-1</sup> for Ni, and 19.7 mg kg<sup>-1</sup> for Zn. Iron was highest in Origanum majorana (96.7 mg kg<sup>-1</sup>), while Cd was lowest in Inula helenium (0.85 mg kg<sup>-1</sup>). For macro elements, the highest values were Ca in Urtica dioica (27,800 mg kg<sup>-1</sup>), K in Echium amoenum (34,600 mg kg<sup>-1</sup>), Mg in Verbascum thapsus (9080 mg kg<sup>-1</sup>), and Na in Borago officinalis (10,500 mg kg<sup>-1</sup>). Relative to adult RDAs, Marrubium vulgare is a key source of Ca (64%) and K (22%), Achillea millefolium provides Mg (98%), Fe (54%), and Mn (97%), and Matricaria chamomilla contributes Cu (86%). The bioaccumulation factor (BCF) averaged 0.68 for Cd, 0.27 for Cu, 0.005 for Fe, 0.26 for Mn, 0.05 for Ni, and 0.44 for Zn, with the highest BCF for Cd (0.92 in Urtica dioica) and lowest for Fe (0.0031 in Marrubium vulgare). The Alcea kurdica, Plantago major, and Urtica dioica may be considered in phytoremediation due to the higher Cd and Ni accumulation. Among studied trace elements, Cd had the highest hazard quotient. This study highlights both the nutritional benefits and safety hazards linked to medicinal plants and proposes some medicinal plants for possible phytoremediation of contaminated soils.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"869-892"},"PeriodicalIF":3.6,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144538031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Determination and Health Risk Assessment of Heavy Metals in Some Canned Food in Tehran, Iran. 伊朗德黑兰部分罐头食品重金属含量测定及健康风险评估。
IF 3.6 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-02-01 Epub Date: 2025-07-09 DOI: 10.1007/s12011-025-04735-6
Ramin Aslani, Parisa Shavali-Gilani, Alireza Bakhtiyari, Parisa Sadighara, Najmeh Yazdanfar, Mahmood Yousefi

Heavy metal exposure has raised grave health concerns globally in recent decades. The objective of the present research was to examine the contents of heavy metals, including arsenic (As), cadmium (Cd), copper (Cu), iron (Fe), mercury (Hg), lead (Pb), tin (Sn), and zinc (Zn) in canned fish, canned chick peas, and canned green peas collected from Tehran, Iran, utilizing inductively coupled plasma optical emission spectroscopy (ICP-OES). Moreover, the health risks of heavy metal exposure through canned sample ingestion were evaluated utilizing Monte-Carlo simulation. The heavy metals in samples were detected in the range of 0.01 - 0.2 mg/kg for As, < LOD - 0.05 mg/kg for Cd, 0.9 - 11.8 mg/kg for Cu, 3 - 12.17 mg/kg for Fe, < LOD - 0.11 mg/kg for Hg, 0.01 - 0.9 mg/kg for Pb, 0.02 - 4.08 mg/kg for Sn, and 2.9 - 7.75 mg/kg for Zn. The mean concentration of metals in samples was within standard limits. THQ and HI values were below 1 and CR values were below 10-6, which were acceptable. These findings suggest that the concentrations of heavy metals in canned fish, chickpeas, and green peas do not pose a health risk to Iranian consumers.

近几十年来,重金属暴露在全球引发了严重的健康问题。利用电感耦合等离子体发射光谱(ICP-OES)技术,对伊朗德黑兰地区采集的鱼罐头、鹰嘴豆罐头和青豆罐头中砷(As)、镉(Cd)、铜(Cu)、铁(Fe)、汞(Hg)、铅(Pb)、锡(Sn)和锌(Zn)等重金属进行了检测。此外,利用蒙特卡罗模拟方法评估了通过罐装样品摄入重金属暴露的健康风险。样品中重金属As, -6的检出范围为0.01 ~ 0.2 mg/kg,可接受。这些发现表明,罐头鱼、鹰嘴豆和青豆中的重金属浓度不会对伊朗消费者构成健康风险。
{"title":"Determination and Health Risk Assessment of Heavy Metals in Some Canned Food in Tehran, Iran.","authors":"Ramin Aslani, Parisa Shavali-Gilani, Alireza Bakhtiyari, Parisa Sadighara, Najmeh Yazdanfar, Mahmood Yousefi","doi":"10.1007/s12011-025-04735-6","DOIUrl":"10.1007/s12011-025-04735-6","url":null,"abstract":"<p><p>Heavy metal exposure has raised grave health concerns globally in recent decades. The objective of the present research was to examine the contents of heavy metals, including arsenic (As), cadmium (Cd), copper (Cu), iron (Fe), mercury (Hg), lead (Pb), tin (Sn), and zinc (Zn) in canned fish, canned chick peas, and canned green peas collected from Tehran, Iran, utilizing inductively coupled plasma optical emission spectroscopy (ICP-OES). Moreover, the health risks of heavy metal exposure through canned sample ingestion were evaluated utilizing Monte-Carlo simulation. The heavy metals in samples were detected in the range of 0.01 - 0.2 mg/kg for As, < LOD - 0.05 mg/kg for Cd, 0.9 - 11.8 mg/kg for Cu, 3 - 12.17 mg/kg for Fe, < LOD - 0.11 mg/kg for Hg, 0.01 - 0.9 mg/kg for Pb, 0.02 - 4.08 mg/kg for Sn, and 2.9 - 7.75 mg/kg for Zn. The mean concentration of metals in samples was within standard limits. THQ and HI values were below 1 and CR values were below 10<sup>-6</sup>, which were acceptable. These findings suggest that the concentrations of heavy metals in canned fish, chickpeas, and green peas do not pose a health risk to Iranian consumers.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"1130-1140"},"PeriodicalIF":3.6,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144590371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of Specific Micronutrients on Semen Quality and Reproductive Outcomes in Peripubertal Sahiwal Bulls. 特定微量营养素对青春期前Sahiwal公牛精液质量和生殖结局的影响。
IF 3.6 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-02-01 Epub Date: 2025-07-08 DOI: 10.1007/s12011-025-04733-8
Vaibhav Dhaigude, Parul Chaudhary, Prasanth M Nair, Hardik Naliyapara, Mukesh Bhakat, Goutam Mondal

Nutritional management with micronutrient supplementation impacts sexual behaviour, health, and semen production in bulls. This study aimed to evaluate the effect of micronutrients on seminal traits, hormonal profile, age at onset of puberty, and reproductive parameters in Sahiwal bull calves. Twenty-four Sahiwal bull calves were divided into four groups of six based on age and body weight. The Control Group received a basal diet (concentrate, green, and dry roughage). Group T1 received the basal diet plus a customised mineral mixture (Zn, Cu, Mn, Cr, and Co). Group T2 received the basal diet plus a customised vitamin mixture (vitamin A and vitamin E). Group T3 received the basal diet plus customised mineral (Zn, Cu, Mn, Cr, and Co) and vitamin (vitamin A and vitamin E) mixture. Seminal traits, hormonal profiles, age at puberty, and reproductive parameters were assessed. Micronutrient supplementation significantly improved seminal characteristics and hormone status in bull calves, and the age of puberty was significantly reduced. Testosterone levels in T3 and T1 were significantly higher after 90 days. Luteinising hormone levels were significantly higher during puberty in T3. Insulin-like growth factor levels remained unaltered. Scrotal biometry indicated a linear increase (p < 0.05) in scrotal circumference, paired testicular weight, and volume. Sexual behaviour indicators did not differ significantly between treatments. The age of puberty was significantly reduced in the T3 group. Micronutrient supplementation beyond basal levels enhances testicular growth, hormone status, and seminal quality in male calves, potentially improving reproductive performance. Micronutrient supplementation beyond the basal level significantly improves testicular growth, hormone status, seminal quality, and overall performance in peripubertal Sahiwal bulls.

补充微量营养素的营养管理影响公牛的性行为、健康和精液产量。本研究旨在评估微量营养素对Sahiwal公牛犊牛精液性状、激素谱、青春期开始年龄和生殖参数的影响。24头萨希瓦尔公牛根据年龄和体重分为四组,每组6头。对照组饲喂基础饲粮(精料、粗料和干粗料)。T1组饲喂基础饲粮加定制矿物混合物(Zn、Cu、Mn、Cr和Co)。T2组饲喂基础饲粮加定制维生素混合物(维生素a和维生素E)。T3组饲喂基础饲粮,外加定制的矿物质(Zn、Cu、Mn、Cr和Co)和维生素(维生素A和维生素E)混合物。评估了精液性状、激素谱、青春期年龄和生殖参数。微量营养素的补充显著改善了公牛犊牛的精液特征和激素状态,并显著降低了青春期的年龄。90天后T3和T1的睾酮水平显著升高。黄体生成素水平在青春期T3期显著升高。胰岛素样生长因子水平保持不变。阴囊生物测量显示线性增加(p
{"title":"Effects of Specific Micronutrients on Semen Quality and Reproductive Outcomes in Peripubertal Sahiwal Bulls.","authors":"Vaibhav Dhaigude, Parul Chaudhary, Prasanth M Nair, Hardik Naliyapara, Mukesh Bhakat, Goutam Mondal","doi":"10.1007/s12011-025-04733-8","DOIUrl":"10.1007/s12011-025-04733-8","url":null,"abstract":"<p><p>Nutritional management with micronutrient supplementation impacts sexual behaviour, health, and semen production in bulls. This study aimed to evaluate the effect of micronutrients on seminal traits, hormonal profile, age at onset of puberty, and reproductive parameters in Sahiwal bull calves. Twenty-four Sahiwal bull calves were divided into four groups of six based on age and body weight. The Control Group received a basal diet (concentrate, green, and dry roughage). Group T1 received the basal diet plus a customised mineral mixture (Zn, Cu, Mn, Cr, and Co). Group T2 received the basal diet plus a customised vitamin mixture (vitamin A and vitamin E). Group T3 received the basal diet plus customised mineral (Zn, Cu, Mn, Cr, and Co) and vitamin (vitamin A and vitamin E) mixture. Seminal traits, hormonal profiles, age at puberty, and reproductive parameters were assessed. Micronutrient supplementation significantly improved seminal characteristics and hormone status in bull calves, and the age of puberty was significantly reduced. Testosterone levels in T3 and T1 were significantly higher after 90 days. Luteinising hormone levels were significantly higher during puberty in T3. Insulin-like growth factor levels remained unaltered. Scrotal biometry indicated a linear increase (p < 0.05) in scrotal circumference, paired testicular weight, and volume. Sexual behaviour indicators did not differ significantly between treatments. The age of puberty was significantly reduced in the T3 group. Micronutrient supplementation beyond basal levels enhances testicular growth, hormone status, and seminal quality in male calves, potentially improving reproductive performance. Micronutrient supplementation beyond the basal level significantly improves testicular growth, hormone status, seminal quality, and overall performance in peripubertal Sahiwal bulls.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"1113-1129"},"PeriodicalIF":3.6,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144582965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cadmium-Induced Testicular and Spermatogonial Damage Ameliorated by Bryonia laciniosa Linn. Seeds in Wistar Rats: An Animal Study. 镉致睾丸和精原损伤的修复研究。Wistar大鼠种子:一项动物研究。
IF 3.6 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-02-01 Epub Date: 2025-07-15 DOI: 10.1007/s12011-025-04737-4
Lisong Chen, Parameswari R, Madhan Kumar P, Babujanarthanam R, Abdurahman Hajinur Hirad, Yinfang Zhou

Cadmium (Cd) is a ubiquitous occupational pollutant that has been extensively studied for its detrimental effects on male reproductive function, primarily through the induction of oxidative stress, which compromises both sperm quality and sperm DNA integrity. This study aimed to evaluate the protective efficacy of Bryonia laciniosa Linn., a phytochemically rich medicinal plant, against cadmium-induced reproductive toxicity in Wistar rats. Twenty-four healthy adult male Wistar rats were randomly divided into four groups (n = 6 per group): a control group, a B. laciniosa extract-treated group, a Cd-treated group, and a combination group that received both Cd and B. laciniosa extract. Rats exposed to Cd exhibited significantly reduced relative testicular weights, accompanied by impaired sperm parameters and altered spermatogonial morphology. Furthermore, Cd exposure led to significantly decreased serum levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH), and testosterone compared to the other groups. Oxidative stress biomarkers revealed that Cd exposure significantly lowered the concentrations of enzymatic antioxidants, such as superoxide dismutase (SOD) and catalase (CAT), while increasing levels of malondialdehyde (MDA) in testicular tissue. Histopathological examination of testicular cross-sections revealed considerable degeneration of spermatogenic cells, characterized by necrotic atrophy and increased eosinophilic fluid discharge in the seminiferous tubules, ultimately impairing reproductive function. Morphometric analysis was conducted using light microscopy. In contrast, treatment with B. laciniosa extract significantly reduced oxidative stress and restored enzyme levels. This restoration facilitated the regeneration of primary spermatids and spermatogonial cells in the combination treatment group, which was attributed to the extract's antioxidant-rich properties, offering protection against free radical-mediated damage and mitigating Cd-induced sperm DNA damage. The observed protective effects were primarily attributed to the synergistic antioxidant activity of B. laciniosa extract.

镉(Cd)是一种普遍存在的职业性污染物,其对男性生殖功能的有害影响已被广泛研究,主要是通过诱导氧化应激,从而损害精子质量和精子DNA完整性。本研究旨在评价青花苔藓的保护作用。一种植物化学成分丰富的药用植物,对镉诱导的Wistar大鼠生殖毒性的影响。选取24只健康成年雄性Wistar大鼠,随机分为4组(每组6只):对照组、白带双歧杆菌提取物组、Cd治疗组和Cd和白带双歧杆菌提取物联合治疗组。暴露于Cd的大鼠表现出睾丸相对重量显著降低,同时精子参数受损和精原细胞形态改变。此外,与其他组相比,Cd暴露导致血清促卵泡激素(FSH)、黄体生成素(LH)和睾酮水平显著降低。氧化应激生物标志物显示,Cd暴露显著降低了酶抗氧化剂的浓度,如超氧化物歧化酶(SOD)和过氧化氢酶(CAT),同时增加了睾丸组织中丙二醛(MDA)的水平。睾丸横断面组织病理学检查显示生精细胞明显变性,表现为坏死萎缩和精小管嗜酸性液体排出增多,最终损害生殖功能。使用光镜进行形态计量学分析。相比之下,白带双歧杆菌提取物显著降低了氧化应激,恢复了酶水平。在联合治疗组中,这种修复促进了原精子和精原细胞的再生,这是由于提取物富含抗氧化剂的特性,提供了对自由基介导的损伤的保护,减轻了cd诱导的精子DNA损伤。所观察到的保护作用主要归因于白叶莲提取物的协同抗氧化活性。
{"title":"Cadmium-Induced Testicular and Spermatogonial Damage Ameliorated by Bryonia laciniosa Linn. Seeds in Wistar Rats: An Animal Study.","authors":"Lisong Chen, Parameswari R, Madhan Kumar P, Babujanarthanam R, Abdurahman Hajinur Hirad, Yinfang Zhou","doi":"10.1007/s12011-025-04737-4","DOIUrl":"10.1007/s12011-025-04737-4","url":null,"abstract":"<p><p>Cadmium (Cd) is a ubiquitous occupational pollutant that has been extensively studied for its detrimental effects on male reproductive function, primarily through the induction of oxidative stress, which compromises both sperm quality and sperm DNA integrity. This study aimed to evaluate the protective efficacy of Bryonia laciniosa Linn., a phytochemically rich medicinal plant, against cadmium-induced reproductive toxicity in Wistar rats. Twenty-four healthy adult male Wistar rats were randomly divided into four groups (n = 6 per group): a control group, a B. laciniosa extract-treated group, a Cd-treated group, and a combination group that received both Cd and B. laciniosa extract. Rats exposed to Cd exhibited significantly reduced relative testicular weights, accompanied by impaired sperm parameters and altered spermatogonial morphology. Furthermore, Cd exposure led to significantly decreased serum levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH), and testosterone compared to the other groups. Oxidative stress biomarkers revealed that Cd exposure significantly lowered the concentrations of enzymatic antioxidants, such as superoxide dismutase (SOD) and catalase (CAT), while increasing levels of malondialdehyde (MDA) in testicular tissue. Histopathological examination of testicular cross-sections revealed considerable degeneration of spermatogenic cells, characterized by necrotic atrophy and increased eosinophilic fluid discharge in the seminiferous tubules, ultimately impairing reproductive function. Morphometric analysis was conducted using light microscopy. In contrast, treatment with B. laciniosa extract significantly reduced oxidative stress and restored enzyme levels. This restoration facilitated the regeneration of primary spermatids and spermatogonial cells in the combination treatment group, which was attributed to the extract's antioxidant-rich properties, offering protection against free radical-mediated damage and mitigating Cd-induced sperm DNA damage. The observed protective effects were primarily attributed to the synergistic antioxidant activity of B. laciniosa extract.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"1159-1170"},"PeriodicalIF":3.6,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144636031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Probing One-Electron Transfer in Selected Trace Elements. 探测选定微量元素的单电子转移。
IF 3.6 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-02-01 Epub Date: 2025-06-26 DOI: 10.1007/s12011-025-04709-8
K Indira Priyadarsini

Electron transfer is a key process in many biological redox reactions like photosynthesis, respiration and peroxidases. Several of these processes are mediated by trace elements like transition metals and chalcogens. Although the overall redox reaction often involves the transfer of multiple electrons, it may happen in several steps of single electron transfer processes. Understanding and probing such one-electron processes is necessary to optimize the energy channeling in the mimetic redox biology. However, monitoring such reactions requires advanced spectroscopic tools. In transition metals, due to the availability of stable oxidation states, one-electron transfer can be monitored by steady-state techniques, like cyclic voltammetry and NMR spectroscopy. But such process involving p-block chalcogen elements like oxygen, sulfur, and selenium requires real-time measurements due to the involvement of short-lived (nano to micro seconds) free radical species. Our group has contributed significantly to the study of one-electron transfer reactions in these systems using a nanosecond pulse radiolysis facility with transient absorption detection and identified crucial steps involved in redox processes in enzyme mimicking reactions. Examples include superoxide dismutase activity of copper-curcumin complexes and glutathione peroxidase activity of small organoselenium compounds; salient features pertaining to electron transfer are discussed in this article.

电子转移是光合作用、呼吸作用和过氧化物酶等生物氧化还原反应的关键过程。其中一些过程是由过渡金属和硫化物等微量元素介导的。虽然整个氧化还原反应通常涉及多个电子的转移,但它也可能发生在单电子转移过程的几个步骤中。了解和探索这种单电子过程对于优化模拟氧化还原生物学中的能量通道是必要的。然而,监测这种反应需要先进的光谱工具。在过渡金属中,由于稳定氧化态的可用性,单电子转移可以通过稳态技术监测,如循环伏安法和核磁共振波谱法。但是,由于涉及到短寿命(纳米到微秒)的自由基物种,这种涉及到氧、硫和硒等p块硫元素的过程需要实时测量。我们的团队在这些系统中使用纳秒脉冲辐射分解设备和瞬态吸收检测的单电子转移反应的研究做出了重大贡献,并确定了酶模拟反应中氧化还原过程的关键步骤。例子包括铜-姜黄素复合物的超氧化物歧化酶活性和小有机硒化合物的谷胱甘肽过氧化物酶活性;本文讨论了与电子转移有关的显著特征。
{"title":"Probing One-Electron Transfer in Selected Trace Elements.","authors":"K Indira Priyadarsini","doi":"10.1007/s12011-025-04709-8","DOIUrl":"10.1007/s12011-025-04709-8","url":null,"abstract":"<p><p>Electron transfer is a key process in many biological redox reactions like photosynthesis, respiration and peroxidases. Several of these processes are mediated by trace elements like transition metals and chalcogens. Although the overall redox reaction often involves the transfer of multiple electrons, it may happen in several steps of single electron transfer processes. Understanding and probing such one-electron processes is necessary to optimize the energy channeling in the mimetic redox biology. However, monitoring such reactions requires advanced spectroscopic tools. In transition metals, due to the availability of stable oxidation states, one-electron transfer can be monitored by steady-state techniques, like cyclic voltammetry and NMR spectroscopy. But such process involving p-block chalcogen elements like oxygen, sulfur, and selenium requires real-time measurements due to the involvement of short-lived (nano to micro seconds) free radical species. Our group has contributed significantly to the study of one-electron transfer reactions in these systems using a nanosecond pulse radiolysis facility with transient absorption detection and identified crucial steps involved in redox processes in enzyme mimicking reactions. Examples include superoxide dismutase activity of copper-curcumin complexes and glutathione peroxidase activity of small organoselenium compounds; salient features pertaining to electron transfer are discussed in this article.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"803-811"},"PeriodicalIF":3.6,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12847218/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144504804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A Cross-sectional Study on Age-Related Changes in Muscle Iron Deposition and Fat Infiltration: Associations with Grip Strength in a Healthy Adult Cohort. 在健康成人队列中,肌肉铁沉积和脂肪浸润年龄相关变化的横断面研究:与握力的关系
IF 3.6 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-02-01 Epub Date: 2025-06-27 DOI: 10.1007/s12011-025-04729-4
Yiping Zhang, Xifa Gao, Rui Yu, Dingzhe Zhang, Yu Wang, Jianhua Wang, Xiao Chen

Both the muscle iron content and fat infiltration are associated with sarcopenia. However, the use of magnetic resonance imaging (MRI) for the quantitative assessment of age-related changes in muscle iron content and fat fraction remains limited. This study investigated sex- and age-related differences in muscle iron content and fat fraction in a healthy population, as well as their relationships with handgrip strength (HGS). A total of 198 participants were included. The muscle iron content and fat fraction in the psoas, erector spinae, multifidus, and combined erector spinae and multifidus were determined via the IDEAL-IQ sequence. HGS was evaluated via an electronic handgrip dynamometer. Low HGS was considered if the HGS was < 27 kg for males and 18 kg for females. The muscle iron content and fat fraction increased with age in both men and women (P < 0.01). These variables were negatively correlated with HGS (P < 0.01). The muscle iron content and fat fraction also had acceptable performance in identifying individuals with low HGS (area under the curve (AUC) = 0.673-0.826 for iron content; AUC = 0.818-0.933 for fat fraction). In conclusion, the muscle iron content and fat fraction both increased with age and were both negatively correlated with HGS, which may be used for assessing and screening for sarcopenia.

肌铁含量和脂肪浸润均与肌少症有关。然而,使用磁共振成像(MRI)定量评估肌肉铁含量和脂肪含量的年龄相关变化仍然有限。这项研究调查了健康人群中肌肉铁含量和脂肪含量的性别和年龄相关差异,以及它们与握力(HGS)的关系。总共包括198名参与者。通过IDEAL-IQ序列测定腰大肌、竖脊肌、多裂肌及联合竖脊肌和多裂肌的铁含量和脂肪含量。HGS通过电子手握测功仪进行评估。低HGS被认为是如果HGS是
{"title":"A Cross-sectional Study on Age-Related Changes in Muscle Iron Deposition and Fat Infiltration: Associations with Grip Strength in a Healthy Adult Cohort.","authors":"Yiping Zhang, Xifa Gao, Rui Yu, Dingzhe Zhang, Yu Wang, Jianhua Wang, Xiao Chen","doi":"10.1007/s12011-025-04729-4","DOIUrl":"10.1007/s12011-025-04729-4","url":null,"abstract":"<p><p>Both the muscle iron content and fat infiltration are associated with sarcopenia. However, the use of magnetic resonance imaging (MRI) for the quantitative assessment of age-related changes in muscle iron content and fat fraction remains limited. This study investigated sex- and age-related differences in muscle iron content and fat fraction in a healthy population, as well as their relationships with handgrip strength (HGS). A total of 198 participants were included. The muscle iron content and fat fraction in the psoas, erector spinae, multifidus, and combined erector spinae and multifidus were determined via the IDEAL-IQ sequence. HGS was evaluated via an electronic handgrip dynamometer. Low HGS was considered if the HGS was < 27 kg for males and 18 kg for females. The muscle iron content and fat fraction increased with age in both men and women (P < 0.01). These variables were negatively correlated with HGS (P < 0.01). The muscle iron content and fat fraction also had acceptable performance in identifying individuals with low HGS (area under the curve (AUC) = 0.673-0.826 for iron content; AUC = 0.818-0.933 for fat fraction). In conclusion, the muscle iron content and fat fraction both increased with age and were both negatively correlated with HGS, which may be used for assessing and screening for sarcopenia.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"1057-1067"},"PeriodicalIF":3.6,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144504801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Boric Acid Diminishes Sciatic Nerve Injury-Induced Apoptosis, Oxidative Stress, and Pain via The Block of TRPV1 Channel in Mice. 硼酸通过阻断小鼠TRPV1通道减少坐骨神经损伤诱导的凋亡、氧化应激和疼痛
IF 3.6 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-02-01 Epub Date: 2025-06-28 DOI: 10.1007/s12011-025-04698-8
Kemal Ertilav, Mustafa Nazıroğlu

The main actors of sciatic nerve injury (SNI) are pain, apoptosis, excessive reactive oxygen species (ROS), and Ca2+ entry. However, the role of antioxidant and antiapoptotic boric acid (BoA) through TRPV1 inhibition on the actors in SNI-induced mice has not yet been elucidated. We investigated whether BoA protected the SNI actors in mice undergoing SNI. The thirty-two mice were divided into four groups: Control, BoA, SNI, and SNI + BoA. For four weeks following SNI induction, the BoA and SNI + BoA received 100 mg/kg BoA intraperitoneally. The SNI group, but not the BoA or BoA + SNI groups, indicated increases in TRPV1 current density and Ca2+ concentration induced by the TRPV1 agonist (capsaicin). The SNI + BoA group had a reduction in the increases of pain intensity (threshold of paw withdrawal and delay of thermal paw withdrawal) induced by SNI. In the brain, blood, and sciatic nerve of the SNI group, BoA and TRPV1 antagonist (capsazepine) treatments reduced the increases of mitochondrial membrane dysfunction, apoptosis, caspases (-3, -8, and -9), lipid peroxidation, mitochondrial, and intracellular ROS caused by SNI through upregulation of cell viability and antioxidants (vitamin A, vitamin E, β-carotene, glutathione, and glutathione peroxidase). In conclusion, BoA therapy reduced the rise in mitochondrial ROS, apoptosis, and Ca2+ entry in the sciatic nerve via inhibiting TRPV1. Therefore, the BoA may be a useful novel treatment through modulation of TRPV1 for oxidative stress, apoptosis, and pain produced by SNI.

坐骨神经损伤(SNI)的主要参与者是疼痛、细胞凋亡、活性氧(ROS)过量和Ca2+进入。然而,抗氧化和抗凋亡硼酸(BoA)通过抑制TRPV1对sni诱导小鼠的作用尚未阐明。我们研究了BoA是否对小鼠SNI行为体有保护作用。将32只小鼠分为4组:Control组、BoA组、SNI组和SNI + BoA组。SNI诱导后4周,BoA和SNI + BoA腹腔注射100 mg/kg BoA。SNI组显示TRPV1激动剂(辣椒素)诱导的TRPV1电流密度和Ca2+浓度升高,而BoA或BoA + SNI组则没有。SNI + BoA组小鼠SNI诱导的疼痛强度(脱爪阈值和热脱爪延迟)的增加明显减少。在SNI组的大脑、血液和坐骨神经中,BoA和TRPV1拮抗剂(辣椒素)处理通过上调细胞活力和抗氧化剂(维生素A、维生素E、β-胡萝卜素、谷胱甘肽和谷胱甘肽过氧化物酶),降低了SNI引起的线粒体膜功能障碍、凋亡、半胱甘肽酶(-3、-8和-9)、脂质过氧化、线粒体和细胞内ROS的增加。综上所述,BoA治疗通过抑制TRPV1降低了坐骨神经线粒体ROS、细胞凋亡和Ca2+进入的增加。因此,BoA可能是通过调节TRPV1来治疗SNI引起的氧化应激、细胞凋亡和疼痛的一种有用的新疗法。
{"title":"Boric Acid Diminishes Sciatic Nerve Injury-Induced Apoptosis, Oxidative Stress, and Pain via The Block of TRPV1 Channel in Mice.","authors":"Kemal Ertilav, Mustafa Nazıroğlu","doi":"10.1007/s12011-025-04698-8","DOIUrl":"10.1007/s12011-025-04698-8","url":null,"abstract":"<p><p>The main actors of sciatic nerve injury (SNI) are pain, apoptosis, excessive reactive oxygen species (ROS), and Ca<sup>2+</sup> entry. However, the role of antioxidant and antiapoptotic boric acid (BoA) through TRPV1 inhibition on the actors in SNI-induced mice has not yet been elucidated. We investigated whether BoA protected the SNI actors in mice undergoing SNI. The thirty-two mice were divided into four groups: Control, BoA, SNI, and SNI + BoA. For four weeks following SNI induction, the BoA and SNI + BoA received 100 mg/kg BoA intraperitoneally. The SNI group, but not the BoA or BoA + SNI groups, indicated increases in TRPV1 current density and Ca<sup>2+</sup> concentration induced by the TRPV1 agonist (capsaicin). The SNI + BoA group had a reduction in the increases of pain intensity (threshold of paw withdrawal and delay of thermal paw withdrawal) induced by SNI. In the brain, blood, and sciatic nerve of the SNI group, BoA and TRPV1 antagonist (capsazepine) treatments reduced the increases of mitochondrial membrane dysfunction, apoptosis, caspases (-3, -8, and -9), lipid peroxidation, mitochondrial, and intracellular ROS caused by SNI through upregulation of cell viability and antioxidants (vitamin A, vitamin E, β-carotene, glutathione, and glutathione peroxidase). In conclusion, BoA therapy reduced the rise in mitochondrial ROS, apoptosis, and Ca<sup>2+</sup> entry in the sciatic nerve via inhibiting TRPV1. Therefore, the BoA may be a useful novel treatment through modulation of TRPV1 for oxidative stress, apoptosis, and pain produced by SNI.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"599-612"},"PeriodicalIF":3.6,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12847084/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144526390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Biological Trace Element Research
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1