Bioengineered最新文献

Anaerobic digestion (AD) is a sustainable technology that converts organic waste into renewable energy while reducing greenhouse gas emissions. Recent studies suggest that adding CO₂ to the AD process can improve methane production through different mechanisms. This review examines four key ways CO₂ supplementation can enhance methane yield: (1) direct conversion of CO₂ into acetate by homoacetogens, (2) direct methanation of CO₂ by hydrogenotrophic methanogens, (3) improved breakdown of organic material due to higher enzyme activity, and (4) better digester conditions through pH regulation and reduced ammonia toxicity. By analyzing microbial interactions and process improvements, this paper highlights knowledge gaps and the need for further research to optimize CO₂ addition in different operational settings. These findings are expected to contribute to the development of cost-effective and efficient AD systems that support energy recovery and environmental sustainability.

Anaerobic fermentation (AF) processes are sensitive to temperature fluctuations, which can influence the microbial activity and overall metabolic performances. Anaerobic reactors can face unforeseen temperature control failures, leading to instabilities in the process. The present study investigated the effect of two short-term temperature perturbations (down to 20°C and 15°C) on AF of food wastes (FWs). While 20°C did not exhibit a negative impact on AF performance maintaining the bioconversion yields over 40%, the reactor subjected to 15°C presented an acidogenic limitation, which decreased the bioconversion yields (36.4 ± 1.8%). As a result, 2.2 ± 0.5 g/L of succinic acid was accumulated in the reactor, being identified as a temperature failure indicator. Once the conditions were reestablished (operation temperature of 25ºC), the metabolic redundancies identified in the reactors allowed the AFs recovery to initial fermentation yields. 20°C was further tested as operational temperature resulting in stable bioconversion yield similar to the Control Reactor (43.2 ± 0.3%). These results showed the feasibility of conducting AF under low temperatures, indicating the potential of this technology to increase the cost-effectiveness of AF at psychrophilic conditions.

Taylor & Francis journal Bioengineered has been targeted by paper mills. Our goal is to identify problematic articles published in Bioengineered during the period 2010 to 2024. Dimensions was used to search for articles that contained the terms 'mouse' OR 'mice' OR 'rat' OR 'rats' in title or abstract, published in Bioengineered between January 1st 2010 to December 31st 2024. All articles were assessed by eye and by using software to detect inappropriate image duplication and manipulation. An article was classified as problematic if it contained inappropriate image duplication or manipulation or had been previously retracted. Problematic articles were reported on PubPeer by the authors if they had not been reported previously. All included articles were assessed for post-publication editorial decisions. We have excluded all articles published in 2024 from further analysis, as these were all retraction notices. We assessed the remaining 878 articles, of which 226 (25.7%) were identified as problematic, of which 35 had been previously retracted. One retracted article was later de-retracted. One article received a correction. None of the included articles received an expression of concern or the Taylor & Francis 'under investigation' pop-up. Taylor & Francis' lack of visible editorial action has left the scientific community vulnerable to reading and citing hundreds of problematic articles published in Bioengineered. To uphold scientific integrity, Taylor & Francis should use the findings of this study as a starting point to systematically identify all compromised articles in Bioengineered and take appropriate editorial action.

Gene editing is emerging as a powerful tool for introducing novel functionalities in mushrooms. While CRISPR/Cas9-induced double-strand breaks (DSBs) typically rely on non-homologous end joining (NHEJ) for gene disruption, precise insertion of heterologous DNA in mushrooms is less explored. Here, we evaluated the efficacy of inserting donor DNAs (8-1008 bp) with or without homologous arms at Cas9-gRNA RNP-induced DSBs. Co-transformation of donor DNAs with RNP targeting the pyrG gene in Ganoderma lucidum yielded 184 transformants without homologous arms and 781 with 300-bp homologous arms (HR_donor DNAs). Restriction analysis and sequencing identified 122 hR_donor DNA transformants with complete donor DNA sequences, achieving 15.6% HDR efficiency (122/781), contrasting with 8 instances via NHEJ from the 184 transformants. These findings highlight the viability of HDR for precise genomic editing in mushrooms, enabling targeted modifications to enhance functionalities.