Pub Date : 2025-12-01Epub Date: 2025-04-23DOI: 10.1080/21655979.2025.2491922
{"title":"Statement of Retraction: LncRNA ASMTL-AS1/microRNA-1270 differentiate prognostic groups in gastric cancer and influence cell proliferation, migration and invasion.","authors":"","doi":"10.1080/21655979.2025.2491922","DOIUrl":"https://doi.org/10.1080/21655979.2025.2491922","url":null,"abstract":"","PeriodicalId":8919,"journal":{"name":"Bioengineered","volume":"16 1","pages":"2491922"},"PeriodicalIF":4.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12026032/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143962771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-01Epub Date: 2025-04-23DOI: 10.1080/21655979.2025.2491959
{"title":"Statement of Retraction: Knockdown of hypoxia-inducible factor 1-alpha (HIF1α) interferes with angiopoietin-like protein 2 (ANGPTL2) to attenuate high glucose-triggered hypoxia/reoxygenation injury in cardiomyocytes.","authors":"","doi":"10.1080/21655979.2025.2491959","DOIUrl":"https://doi.org/10.1080/21655979.2025.2491959","url":null,"abstract":"","PeriodicalId":8919,"journal":{"name":"Bioengineered","volume":"16 1","pages":"2491959"},"PeriodicalIF":4.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12026171/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143973783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-01Epub Date: 2025-04-23DOI: 10.1080/21655979.2025.2491941
{"title":"Statement of Retraction: CircSLC7A6 promotes the progression of Wilms' tumor via microRNA-107/ ABL proto-oncogene 2 axis.","authors":"","doi":"10.1080/21655979.2025.2491941","DOIUrl":"https://doi.org/10.1080/21655979.2025.2491941","url":null,"abstract":"","PeriodicalId":8919,"journal":{"name":"Bioengineered","volume":"16 1","pages":"2491941"},"PeriodicalIF":4.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12026220/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143975968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-01Epub Date: 2025-04-23DOI: 10.1080/21655979.2025.2491957
{"title":"Statement of Retraction: LncRNA SNHG12 in extracellular vesicles derived from carcinoma-associated fibroblasts promotes cisplatin resistance in non-small cell lung cancer cells.","authors":"","doi":"10.1080/21655979.2025.2491957","DOIUrl":"https://doi.org/10.1080/21655979.2025.2491957","url":null,"abstract":"","PeriodicalId":8919,"journal":{"name":"Bioengineered","volume":"16 1","pages":"2491957"},"PeriodicalIF":4.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12026038/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-01Epub Date: 2025-01-29DOI: 10.1080/21655979.2025.2458376
Hyerang Eom, Yeon-Jae Choi, Rutuja Nandre, Minseek Kim, Youn-Lee Oh, Sinil Kim, Takehito Nakazawa, Yoichi Honda, Hyeon-Su Ro
Gene editing is emerging as a powerful tool for introducing novel functionalities in mushrooms. While CRISPR/Cas9-induced double-strand breaks (DSBs) typically rely on non-homologous end joining (NHEJ) for gene disruption, precise insertion of heterologous DNA in mushrooms is less explored. Here, we evaluated the efficacy of inserting donor DNAs (8-1008 bp) with or without homologous arms at Cas9-gRNA RNP-induced DSBs. Co-transformation of donor DNAs with RNP targeting the pyrG gene in Ganoderma lucidum yielded 184 transformants without homologous arms and 781 with 300-bp homologous arms (HR_donor DNAs). Restriction analysis and sequencing identified 122 hR_donor DNA transformants with complete donor DNA sequences, achieving 15.6% HDR efficiency (122/781), contrasting with 8 instances via NHEJ from the 184 transformants. These findings highlight the viability of HDR for precise genomic editing in mushrooms, enabling targeted modifications to enhance functionalities.
{"title":"Targeted insertion of heterogenous DNA using Cas9-gRNA ribonucleoprotein-mediated gene editing in <i>Ganoderma lucidum</i>.","authors":"Hyerang Eom, Yeon-Jae Choi, Rutuja Nandre, Minseek Kim, Youn-Lee Oh, Sinil Kim, Takehito Nakazawa, Yoichi Honda, Hyeon-Su Ro","doi":"10.1080/21655979.2025.2458376","DOIUrl":"10.1080/21655979.2025.2458376","url":null,"abstract":"<p><p>Gene editing is emerging as a powerful tool for introducing novel functionalities in mushrooms. While CRISPR/Cas9-induced double-strand breaks (DSBs) typically rely on non-homologous end joining (NHEJ) for gene disruption, precise insertion of heterologous DNA in mushrooms is less explored. Here, we evaluated the efficacy of inserting donor DNAs (8-1008 bp) with or without homologous arms at Cas9-gRNA RNP-induced DSBs. Co-transformation of donor DNAs with RNP targeting the <i>pyrG</i> gene in <i>Ganoderma lucidum</i> yielded 184 transformants without homologous arms and 781 with 300-bp homologous arms (HR_donor DNAs). Restriction analysis and sequencing identified 122 hR_donor DNA transformants with complete donor DNA sequences, achieving 15.6% HDR efficiency (122/781), contrasting with 8 instances via NHEJ from the 184 transformants. These findings highlight the viability of HDR for precise genomic editing in mushrooms, enabling targeted modifications to enhance functionalities.</p>","PeriodicalId":8919,"journal":{"name":"Bioengineered","volume":"16 1","pages":"2458376"},"PeriodicalIF":4.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11781247/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143057945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-01Epub Date: 2025-07-21DOI: 10.1080/21655979.2025.2529670
Madhu Subramani, K Suthindhiran
Seventeen halotolerant bacteria were isolated from the Muthupettai mangroves, Tamil Nadu, India, with eight exhibiting protease production. The most potent isolate, VITGS4, identified as Streptomyces sp. via polyphasic taxonomy, yielded 470 U mL-1. Response surface methodology (RSM) optimized protease production by Box-Behnken Design (BBD) using casein (5.5% w/v), pH 7.5, and 9.5 days incubation, achieving 282 U mL-1. The recovered protease was partially purified through acetone precipitation (50% acetone), followed by dialysis, and its purity was estimated through HPLC (high pressure liquid chromatography). Enzyme kinetics revealed a Km of 0.347 µM, a Vo of 0.464 µM min-1, a Vmax of 3.167 µM min-1, and a Kcat of 0.0002 min-1. The enzyme was identified as a halo-thermo-alkaline serine protease, optimally active at pH 8 and 45°C, with activity significantly inhibited by Pb2+ and Hg2+ and enhanced by Zn2+ (95%). Notably, PMSF strongly inhibited protease activity, indicating a serine protease. This protease was successfully employed to recover 726 mg of silver slurry (537 µg mL-1 silver) from X-ray films. Scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX) confirmed the presence of silver (2.2% in the analyzed region), while zeta potential (-26.35 mV) and hydrodynamic diameter (89.94 nm) analyses indicated stable silver nanoparticles. These results demonstrate the potential of marine actinobacteria-derived proteases for efficient silver recovery, offering promising applications in therapeutic and industrial fields.
从印度泰米尔纳德邦的Muthupettai红树林中分离出17种耐盐细菌，其中8种具有蛋白酶生产能力。最有效的分离物VITGS4经多相分类鉴定为Streptomyces sp.，产率为470 U mL-1。响应面法（RSM）通过Box-Behnken设计（BBD）优化了酪蛋白（5.5% w/v）、pH 7.5和9.5 d孵育下的蛋白酶产量，达到282 U mL-1。回收的蛋白酶通过丙酮沉淀（50%丙酮）部分纯化，透析，并通过HPLC（高压液相色谱）估计其纯度。酶动力学显示Km为0.347µM， Vo为0.464µM min-1， Vmax为3.167µM min-1， Kcat为0.0002 min-1。该酶被鉴定为一种晕热碱性丝氨酸蛋白酶，在pH 8和45°C时活性最佳，Pb2+和Hg2+显著抑制其活性，Zn2+增强其活性（95%）。值得注意的是，PMSF强烈抑制蛋白酶活性，表明是丝氨酸蛋白酶。该蛋白酶成功地从x射线胶片中回收了726 mg银浆（537µg mL-1银）。扫描电镜（SEM）和能量色散x射线能谱（EDX）证实了银的存在（2.2%），而zeta电位（-26.35 mV）和流体动力直径（89.94 nm）分析表明银纳米粒子是稳定的。这些结果表明，海洋放线菌衍生的蛋白酶具有高效回收银的潜力，在治疗和工业领域具有广阔的应用前景。
{"title":"Enzymatic recovery of nano-silver from used X-ray films by alkaline protease from <i>Streptomyces</i> sp. VITGSS4.","authors":"Madhu Subramani, K Suthindhiran","doi":"10.1080/21655979.2025.2529670","DOIUrl":"10.1080/21655979.2025.2529670","url":null,"abstract":"<p><p>Seventeen halotolerant bacteria were isolated from the Muthupettai mangroves, Tamil Nadu, India, with eight exhibiting protease production. The most potent isolate, VITGS4, identified as <i>Streptomyces</i> sp. via polyphasic taxonomy, yielded 470 U mL<sup>-1</sup>. Response surface methodology (RSM) optimized protease production by Box-Behnken Design (BBD) using casein (5.5% w/v), pH 7.5, and 9.5 days incubation, achieving 282 U mL<sup>-1</sup>. The recovered protease was partially purified through acetone precipitation (50% acetone), followed by dialysis, and its purity was estimated through HPLC (high pressure liquid chromatography). Enzyme kinetics revealed a Km of 0.347 µM, a Vo of 0.464 µM min<sup>-1</sup>, a Vmax of 3.167 µM min<sup>-1</sup>, and a Kcat of 0.0002 min<sup>-1</sup>. The enzyme was identified as a halo-thermo-alkaline serine protease, optimally active at pH 8 and 45°C, with activity significantly inhibited by Pb<sup>2+</sup> and Hg<sup>2+</sup> and enhanced by Zn<sup>2+</sup> (95%). Notably, PMSF strongly inhibited protease activity, indicating a serine protease. This protease was successfully employed to recover 726 mg of silver slurry (537 µg mL<sup>-1</sup> silver) from X-ray films. Scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX) confirmed the presence of silver (2.2% in the analyzed region), while zeta potential (-26.35 mV) and hydrodynamic diameter (89.94 nm) analyses indicated stable silver nanoparticles. These results demonstrate the potential of marine actinobacteria-derived proteases for efficient silver recovery, offering promising applications in therapeutic and industrial fields.</p>","PeriodicalId":8919,"journal":{"name":"Bioengineered","volume":"16 1","pages":"2529670"},"PeriodicalIF":4.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12296066/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144681892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-01Epub Date: 2025-04-23DOI: 10.1080/21655979.2025.2491940
{"title":"Statement of Retraction: Sulforaphane ameliorates amyloid-β-induced inflammatory injury by suppressing the PARP1/SIRT1 pathway in retinal pigment epithelial cells.","authors":"","doi":"10.1080/21655979.2025.2491940","DOIUrl":"https://doi.org/10.1080/21655979.2025.2491940","url":null,"abstract":"","PeriodicalId":8919,"journal":{"name":"Bioengineered","volume":"16 1","pages":"2491940"},"PeriodicalIF":4.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12026186/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143969404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-01Epub Date: 2025-04-23DOI: 10.1080/21655979.2025.2491956
{"title":"Statement of Retraction: Identification of a novel circular RNA circZNF652/miR-486-5p/SERPINE1 signaling cascade that regulates cancer aggressiveness in glioblastoma (GBM).","authors":"","doi":"10.1080/21655979.2025.2491956","DOIUrl":"https://doi.org/10.1080/21655979.2025.2491956","url":null,"abstract":"","PeriodicalId":8919,"journal":{"name":"Bioengineered","volume":"16 1","pages":"2491956"},"PeriodicalIF":4.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12026130/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143970972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
扫码关注我们
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号