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Cutaneotrichosporon curvatum and Yarrowia lipolytica as key players for green chemistry: efficient oil producers from food waste via the carboxylate platform. 曲皮三磷酸体和脂性耶氏菌是绿色化学的关键参与者:通过羧酸盐平台从食物垃圾中高效地生产石油。
IF 4.9 4区 生物学 Q2 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-12-01 Epub Date: 2023-11-27 DOI: 10.1080/21655979.2023.2286723
Elia Tomás-Pejó, Sergio Morales-Palomo, Cristina González-Fernández

Cutaneotrichosporon curvatum and Yarrowia lipolytica can accumulate microbial oils using short-chain fatty acids (SCFA) as carbon sources. SCFAs-rich media often contain significant amounts of nitrogen that prevent high carbon:nitrogen (C:N) ratios necessary to boost lipid production. This work assessed the intrinsic ability of C. curvatum and Y. lipolytica to produce high amounts of microbial oils from these unusual carbon sources. Results demonstrated that minor differences in SCFA concentration (only 2 g/L) had a significant effect on yeast growth and lipid production. A C:N of 80 promoted yeast growth at all SCFA concentrations and favored SCFA consumption at 19 g/L SCFAs. The different SCFA uptake preferences in C. curvatum and Y. lipolytica highlighted the importance of considering the SCFA profile to select a suitable yeast strain for microbial oils production. At the most challenging SCFA concentration (19 g/L), 57.2% ±1.6% (w/w) and 78.4 ± 0.6% (w/w) lipid content were obtained in C. curvatum and Y. lipolytica, respectively. These values are among the highest reported for wild-type strains. To circumvent the challenges associated with media with high nitrogen content, this report also proved struvite precipitation as an effective method for increasing lipid production (from 17.9 ± 3.9% (w/w) to 41.9 ± 2.6% (w/w)) after nitrogen removal in food waste-derived media.

曲面三磷酸体和多脂耶氏菌可以利用短链脂肪酸作为碳源积累微生物油脂。富含scfa的培养基通常含有大量的氮,这阻止了促进脂质生成所需的高碳:氮(C:N)比。这项工作评估了弯曲菌和脂质体菌从这些不寻常的碳源中产生大量微生物油的内在能力。结果表明,SCFA浓度的微小差异(仅2 g/L)对酵母生长和脂质产生显著影响。80的碳氮比促进了所有SCFA浓度下的酵母生长,并有利于19 g/L SCFA时的SCFA消耗。弯曲酵母和脂肪瘤酵母不同的SCFA摄取偏好突出了考虑SCFA特征选择适合微生物油脂生产的酵母菌株的重要性。在最具挑战性的SCFA浓度(19 g/L)下,曲率C.和脂质Y.的脂质含量分别为57.2%±1.6% (w/w)和78.4±0.6% (w/w)。这些值是报道的野生型菌株中最高的。为了避免与高氮含量培养基相关的挑战,本报告还证明鸟粪石沉淀是一种有效的方法,可以在食物垃圾来源的培养基中去除氮后提高脂质产量(从17.9±3.9% (w/w)增加到41.9±2.6% (w/w))。
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引用次数: 0
Statement of Retraction: The long non-coding RNA ASMTL-AS1 promotes hepatocellular carcinoma progression by sponging miR-1343-3p that suppresses LAMC1 (laminin subunit gamma 1). 撤回声明:长非编码RNA ASMTL-AS1通过吸收抑制LAMC1(层粘连蛋白亚单位γ1)的miR-1343-3p来促进肝细胞癌的进展。
IF 4.9 4区 生物学 Q2 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-12-01 Epub Date: 2023-10-18 DOI: 10.1080/21655979.2023.2269349
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引用次数: 0
Statement of Retraction: Circular RNA protein tyrosine kinase 2 (circPTK2) promotes colorectal cancer proliferation, migration, invasion and chemoresistance. 撤回声明:环状RNA蛋白酪氨酸激酶2(circPTK2)促进癌症增殖、迁移、侵袭和化疗耐药性。
IF 4.9 4区 生物学 Q2 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-12-01 Epub Date: 2023-10-18 DOI: 10.1080/21655979.2023.2269352
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引用次数: 0
Modified polymer membranes for the removal of pharmaceutical active compounds in wastewater and its mechanism-A review. 改性聚合物膜去除废水中药物活性化合物及其机理研究进展。
IF 4.2 4区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2023-12-01 DOI: 10.1080/21655979.2023.2252234
Anisa Ratnasari

Membrane technology can play a suitable role in removing pharmaceutical active compounds since it requires low energy and simple operation. Even though membrane technology has progressed for wastewater applications nowadays, modifying membranes to achieve the strong desired membrane performance is still needed. Thus, this study overviews a comprehensive insight into the application of modified polymer membranes to remove pharmaceutical active compounds from wastewater. Biotoxicity of pharmaceutical active compounds is first prescribed to gain deep insight into how membranes can remove pharmaceutical active compounds from wastewater. Then, the behavior of the diffusion mechanism can be concisely determined using mass transfer factor model that represented by β and B with value up to 2.004 g h mg-1 and 1.833 mg g-1 for organic compounds including pharmaceutical active compounds. The model refers to the adsorption of solute to attach onto acceptor sites of the membrane surface, external mass transport of solute materials from the bulk liquid to the membrane surface, and internal mass transfer to diffuse a solute toward acceptor sites of the membrane surface with evidenced up to 0.999. Different pharmaceutical compounds have different solubility and relates to the membrane hydrophilicity properties and mechanisms. Ultimately, challenges and future recommendations have been presented to view the future need to enhance membrane performance regarding fouling mitigation and recovering compounds. Afterwards, the discussion of this study is projected to play a critical role in advance of better-quality membrane technologies for removing pharmaceutical active compounds from wastewater in an eco-friendly strategy and without damaging the ecosystem.

膜技术可以在去除药物活性化合物方面发挥适当的作用,因为它需要低能量和简单的操作。尽管如今膜技术在废水应用中取得了进展,但仍需要对膜进行改性以获得所需的强膜性能。因此,本研究综述了改性聚合物膜在去除废水中药物活性化合物方面的应用。药物活性化合物的生物毒性是为了深入了解膜如何从废水中去除药物活性化合物而首次提出的。然后,使用以β和B表示的传质因子模型,可以简明地确定扩散机制的行为,其值高达2.004 g h mg-1和1.833 mg g-1用于包括药物活性化合物的有机化合物。该模型指的是溶质吸附到膜表面的受体位点上,溶质材料从本体液体到膜表面上的外部质量传输,以及溶质向膜表面受体位点扩散的内部质量转移,证明高达0.999。不同的药物化合物具有不同的溶解度,并与膜的亲水性性质和机理有关。最终,提出了挑战和未来的建议,以考虑未来在减轻污染和回收化合物方面提高膜性能的需要。之后,本研究的讨论预计将在推进更高质量的膜技术方面发挥关键作用,以环保的策略从废水中去除药物活性化合物,而不会破坏生态系统。
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引用次数: 0
Recent advances in microbial engineering approaches for wastewater treatment: a review. 废水处理微生物工程方法的最新进展:综述。
IF 4.2 4区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2023-12-01 DOI: 10.1080/21655979.2023.2184518
Monika Sharma, Sangita Agarwal, Richa Agarwal Malik, Gaurav Kumar, Dan Bahadur Pal, Mamun Mandal, Abhijit Sarkar, Farkad Bantun, Shafiul Haque, Pardeep Singh, Neha Srivastava, Vijai Kumar Gupta

In the present era of global climate change, the scarcity of potable water is increasing both due to natural and anthropogenic causes. Water is the elixir of life, and its usage has risen significantly due to escalating economic activities, widespread urbanization, and industrialization. The increasing water scarcity and rising contamination have compelled, scientists and researchers, to adopt feasible and sustainable wastewater treatment methods in meeting the growing demand for freshwater. Presently, various waste treatment technologies are adopted across the globe, such as physical, chemical, and biological treatment processes. There is a need to replace these technologies with sustainable and green technology that encourages the use of microorganisms since they have proven to be more effective in water treatment processes. The present review article is focused on demonstrating how effectively various microbes can be used in wastewater treatment to achieve environmental sustainability and economic feasibility. The microbial consortium used for water treatment offers many advantages over pure culture. There is an urgent need to develop hybrid treatment technology for the effective remediation of various organic and inorganic pollutants from wastewater.

在当前全球气候变化的时代,由于自然和人为原因,饮用水的稀缺性正在增加。水是长生不老药,由于不断升级的经济活动、广泛的城市化和工业化,水的使用量大幅增加。日益严重的水资源短缺和日益严重的污染迫使科学家和研究人员采用可行和可持续的废水处理方法来满足日益增长的淡水需求。目前,全球采用了各种废物处理技术,如物理、化学和生物处理工艺。有必要用可持续的绿色技术取代这些技术,鼓励使用微生物,因为它们已被证明在水处理过程中更有效。这篇综述文章的重点是证明如何有效地将各种微生物用于废水处理,以实现环境可持续性和经济可行性。与纯培养相比,用于水处理的微生物群落具有许多优点。迫切需要开发混合处理技术来有效修复废水中的各种有机和无机污染物。
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引用次数: 0
Design, optimization, production and activity testing of recombinant immunotoxins expressed in plants and plant cells for the treatment of monocytic leukemia. 用于治疗单核细胞白血病的植物和植物细胞表达的重组免疫毒素的设计、优化、生产和活性测试。
IF 4.9 4区 生物学 Q2 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-12-01 DOI: 10.1080/21655979.2023.2244235
Matthias Knödler, Katharina Frank, Lucy Kerpen, Johannes Felix Buyel

Antibody-drug conjugates (ADCs) can improve therapeutic indices compared to plain monoclonal antibodies (mAbs). However, ADC synthesis is complex because the components are produced separately in CHO cells (mAb) and often by chemical synthesis (drug). They are individually purified, coupled, and then the ADC is purified, increasing production costs compared to regular mAbs. In contrast, it is easier to produce recombinant fusion proteins consisting of an antibody derivative, linker and proteinaceous toxin, i.e. a recombinant immunotoxin (RIT). Plants are capable of the post-translational modifications needed for functional antibodies and can also express active protein toxins such as the recombinant mistletoe lectin viscumin, which is not possible in prokaryotes and mammalian cells respectively. Here, we used Nicotiana benthamiana and N. tabacum plants as well as tobacco BY-2 cell-based plant cell packs (PCPs) to produce effective RITs targeting CD64 as required for the treatment of myelomonocytic leukemia. We compared RITs with different subcellular targeting signals, linkers, and proteinaceous toxins. The accumulation of selected candidates was improved to ~ 40 mg kg-1 wet biomass using a design of experiments approach, and corresponding proteins were isolated with a purity of ~ 80% using an optimized affinity chromatography method with an overall yield of ~ 84%. One anti-CD64 targeted viscumin-based drug candidate was characterized in terms of storage stability and cytotoxicity test in vitro using human myelomonocytic leukemia cell lines. We identified bottlenecks in the plant-based expression platform that require further improvement and assessed critical process parameters that should be considered during process development for plant-made RITs.

与普通单克隆抗体(mAbs)相比,抗体-药物偶联物(ADC)可以提高治疗指标。然而,ADC合成是复杂的,因为这些成分在CHO细胞(mAb)中单独产生,并且通常通过化学合成(药物)产生。它们被单独纯化、偶联,然后ADC被纯化,与常规mAb相比增加了生产成本。相反,更容易产生由抗体衍生物、接头和蛋白质毒素组成的重组融合蛋白,即重组免疫毒素(RIT)。植物能够进行功能性抗体所需的翻译后修饰,还可以表达活性蛋白毒素,如重组槲寄生凝集素内脏素,这在原核生物和哺乳动物细胞中是不可能的。在这里,我们使用本氏烟草(Nicotiana benthamiana)和烟草(N.tabacum)植物以及基于烟草BY-2细胞的植物细胞包(PCPs)来产生治疗骨髓单核细胞白血病所需的靶向CD64的有效RIT。我们将RIT与不同的亚细胞靶向信号、连接体和蛋白质毒素进行了比较。所选候选人的积累提高到 ~ 40 mg kg-1湿生物量,采用实验设计的方法,分离出纯度为 ~ 80%,使用优化的亲和层析方法,总产率为 ~ 84%。使用人骨髓单核细胞白血病细胞系,对一种抗CD64靶向的基于粘蛋白的候选药物在体外储存稳定性和细胞毒性测试方面进行了表征。我们确定了植物表达平台中需要进一步改进的瓶颈,并评估了在植物制造RIT的工艺开发过程中应考虑的关键工艺参数。
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引用次数: 1
Databases for biomass and waste biorefinery - a mini-review and SWOT analysis. 生物质和废物生物炼制数据库-一个小型回顾和SWOT分析。
IF 4.9 4区 生物学 Q2 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-12-01 Epub Date: 2023-11-29 DOI: 10.1080/21655979.2023.2286722
Morgen Mukamwi, Tosin Somorin, Raimonda Soloha, Elina Dace

The world is facing problems of the increasing amount of resources wasted as the world population grows. Biowaste streams form a significant part of the overall waste generation, and a circular economy utilizing this biowaste will significantly reduce waste whilst lowering the anthropogenic carbon footprint. Due to their energy content and high concentration of hydrocarbon molecules, bio-based waste streams have the potential to be transformed into valorized products (energy, fuels, and chemicals) using biorefinery technologies. In this work, a mini-review has been conducted on available, mostly European databases on existing biomass types and biorefinery technologies to provide a framework for a desirable, comprehensive database connecting bio-based waste streams, biorefinery technologies and bioproducts, as well as the geographical distribution of feedstocks and biorefineries. The database assessment utilized the SWOT (strengths, weakness, opportunities, threats) methodology to support benchmark analysis and to identify critical gaps in underlying data structures that could be included in a single database. The results show that current databases are useful but insufficient for waste biorefineries due to limited quality and quantity as well as the usability of data. A comprehensive database or improved database cluster would be necessary, not only for technology development but for better investment and policy decisions. The development of the new database architecture would need to incorporate the aspects: expansion of database scope and content depth, improved usability, accessibility, applicability, update frequency, openness to new contributions, process descriptions and parameters, and technology readiness level.

随着世界人口的增长,世界正面临着越来越多的资源浪费问题。生物废物流构成了整个废物产生的重要组成部分,利用这种生物废物的循环经济将大大减少废物,同时降低人为碳足迹。由于其能量含量和高浓度的碳氢化合物分子,生物基废物流有可能通过生物炼制技术转化为有价产品(能源、燃料和化学品)。在这项工作中,对现有的主要是欧洲的关于现有生物量类型和生物炼制技术的数据库进行了小型审查,以提供一个框架,建立一个理想的综合数据库,将基于生物的废物流、生物炼制技术和生物产品以及原料和生物炼制的地理分布联系起来。数据库评估使用SWOT(优势、劣势、机会、威胁)方法来支持基准分析,并确定可以包含在单个数据库中的底层数据结构中的关键差距。结果表明,目前的数据库是有用的,但由于数据的质量和数量以及可用性有限,对废物生物精炼厂来说还不够。不仅对于技术发展,而且对于更好的投资和政策决定,一个全面的数据库或改进的数据库集群都是必要的。新数据库体系结构的开发需要结合以下方面:数据库范围和内容深度的扩展、改进的可用性、可访问性、适用性、更新频率、对新贡献的开放性、过程描述和参数,以及技术准备水平。
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引用次数: 0
Influence of exogenous polyamines on the secondary somatic embryogenesis of cork oak (Quercus suber L.). 外源多胺对栓皮栎次生体细胞胚胎发生的影响。
IF 4.9 4区 生物学 Q2 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-12-01 Epub Date: 2023-11-29 DOI: 10.1080/21655979.2023.2288354
Naouar Ben Ali, Rajae Benkaddour, Safaa Rahmouni, Ouafaa Hamdoun, Ibtissam Boussaoudi, Mustapha Hassoun, Latifa Azaroual, Alain Badoc, Patrick Martin, Ahmed Lamarti

Quercus suber L. is the main woody tree species in the Mediterranean basin. The in vitro regeneration from adult material, through primary somatic embryogenesis, is a well-known process, but the use of secondary somatic embryos for plant regeneration remains a very sparsely studied process. The main objective of this work is to explore the cork oak regeneration potential by using the secondary somatic embryogenesis process. Mainly, in this work, we report the polyamine effect. Explants used consisted on primary mature embryos, derived from leaves rejuvenated by epicormic shoot of the Moroccan Quercus suber. Three different polyamines were added to the basal medium, which was composed by macronutrients of N30K, 30 g/l glucose, and 7 g/l agar. Three polyamines, Putrescine, Spermine, and Spermidine, were added to the basal medium at 0.1, 0.2, 0.3, 0.4, 0.5, and 0.6 mg/l. Explants were tested after 8 weeks. Morphological analysis showed that the medium with 0.4 mg/l Spermidine provided the best result for secondary embryos, which corresponds to a very significant (p < 0.05) increase of 375%. The number of secondary embryos directly formed was 2.70 ± 0.51. Similarly, the optimum concentrations for high number of clusters (0.50 ± 0.11) and embryo clusters (1.43 ± 0.35) were increased by 145% and 158%. The addition of the polyamine also acted on the quality of embryos formed. A very significant (p < 0.05) increase in the size of secondary embryos was observed compared to the medium without polyamines. Spermidine showed the greatest increase (about 38%).

Quercus subber L.是地中海盆地的主要木本树种。成体材料通过初生体细胞胚胎的离体再生是一个众所周知的过程,但利用次生体细胞胚胎进行植物再生仍然是一个非常稀少的研究过程。本研究的主要目的是利用次生体细胞胚胎发生过程探索栓皮栎的再生潜力。在本工作中,我们主要报道了多胺效应。所使用的外植体包括初生成熟胚,由摩洛哥栎亚种的表皮芽再生而来。将三种不同的多胺添加到由N30K、30 g/l葡萄糖和7 g/l琼脂组成的基础培养基中。将腐胺、精胺和亚精胺三种多胺分别以0.1、0.2、0.3、0.4、0.5和0.6 mg/l的浓度加入基础培养基。8周后检测外植体。形态学分析表明,亚精胺浓度为0.4 mg/l的培养基对继发胚胎的培养效果最好,与对照差异极显著(p < 0.05)
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引用次数: 0
Sustainable circular biorefinery approach for novel building blocks and bioenergy production from algae using microbial fuel cell. 利用微生物燃料电池从藻类中生产新的构建块和生物能源的可持续循环生物精炼方法。
IF 4.2 4区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2023-12-01 DOI: 10.1080/21655979.2023.2236842
Kevin Tian Xiang Tong, Inn Shi Tan, Henry Chee Yew Foo, Pau Loke Show, Man Kee Lam, Mee Kee Wong

The imminent need for transition to a circular biorefinery using microbial fuel cells (MFC), based on the valorization of renewable resources, will ameliorate the carbon footprint induced by industrialization. MFC catalyzed by bioelectrochemical process drew significant attention initially for its exceptional potential for integrated production of biochemicals and bioenergy. Nonetheless, the associated costly bioproduct production and slow microbial kinetics have constrained its commercialization. This review encompasses the potential and development of macroalgal biomass as a substrate in the MFC system for L-lactic acid (L-LA) and bioelectricity generation. Besides, an insight into the state-of-the-art technological advancement in the MFC system is also deliberated in detail. Investigations in recent years have shown that MFC developed with different anolyte enhances power density from several µW/m2 up to 8160 mW/m2. Further, this review provides a plausible picture of macroalgal-based L-LA and bioelectricity circular biorefinery in the MFC system for future research directions.

基于可再生资源的定价,迫切需要过渡到使用微生物燃料电池(MFC)的循环生物精炼厂,这将改善工业化带来的碳足迹。生物电化学过程催化的MFC最初因其在生物化学品和生物能源综合生产方面的特殊潜力而引起人们的极大关注。尽管如此,相关的昂贵的生物产品生产和缓慢的微生物动力学限制了其商业化。本文综述了大型藻类生物量作为L-乳酸(L-LA)和生物发电MFC系统中的底物的潜力和发展。此外,还详细介绍了MFC系统中最先进的技术进步。近年来的研究表明,使用不同阳极电解液开发的MFC可将功率密度从几µW/m2提高到8160 mW/m2。此外,这篇综述为未来的研究方向提供了基于大型藻类的L-LA和MFC系统中的生物电循环生物精炼的合理图景。
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引用次数: 0
Statement of Retraction: N(6)-adenosine-methyltransferase-14 promotes glioma tumorigenesis by repressing argininosuccinate synthase 1 expression in an m6A-dependent manner. 撤回声明:N(6)-腺苷-甲基转移酶-14通过以m6A依赖的方式抑制精氨酸琥珀酸合酶1的表达,促进神经胶质瘤的发生。
IF 4.9 4区 生物学 Q2 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-12-01 Epub Date: 2023-10-18 DOI: 10.1080/21655979.2023.2269351
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引用次数: 0
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