Cutaneotrichosporon curvatum and Yarrowia lipolytica can accumulate microbial oils using short-chain fatty acids (SCFA) as carbon sources. SCFAs-rich media often contain significant amounts of nitrogen that prevent high carbon:nitrogen (C:N) ratios necessary to boost lipid production. This work assessed the intrinsic ability of C. curvatum and Y. lipolytica to produce high amounts of microbial oils from these unusual carbon sources. Results demonstrated that minor differences in SCFA concentration (only 2 g/L) had a significant effect on yeast growth and lipid production. A C:N of 80 promoted yeast growth at all SCFA concentrations and favored SCFA consumption at 19 g/L SCFAs. The different SCFA uptake preferences in C. curvatum and Y. lipolytica highlighted the importance of considering the SCFA profile to select a suitable yeast strain for microbial oils production. At the most challenging SCFA concentration (19 g/L), 57.2% ±1.6% (w/w) and 78.4 ± 0.6% (w/w) lipid content were obtained in C. curvatum and Y. lipolytica, respectively. These values are among the highest reported for wild-type strains. To circumvent the challenges associated with media with high nitrogen content, this report also proved struvite precipitation as an effective method for increasing lipid production (from 17.9 ± 3.9% (w/w) to 41.9 ± 2.6% (w/w)) after nitrogen removal in food waste-derived media.
{"title":"<i>Cutaneotrichosporon curvatum</i> and <i>Yarrowia lipolytica</i> as key players for green chemistry: efficient oil producers from food waste via the carboxylate platform.","authors":"Elia Tomás-Pejó, Sergio Morales-Palomo, Cristina González-Fernández","doi":"10.1080/21655979.2023.2286723","DOIUrl":"10.1080/21655979.2023.2286723","url":null,"abstract":"<p><p><i>Cutaneotrichosporon curvatum and Yarrowia lipolytica</i> can accumulate microbial oils using short-chain fatty acids (SCFA) as carbon sources. SCFAs-rich media often contain significant amounts of nitrogen that prevent high carbon:nitrogen (C:N) ratios necessary to boost lipid production. This work assessed the intrinsic ability of <i>C. curvatum</i> and <i>Y. lipolytica</i> to produce high amounts of microbial oils from these unusual carbon sources. Results demonstrated that minor differences in SCFA concentration (only 2 g/L) had a significant effect on yeast growth and lipid production. A C:N of 80 promoted yeast growth at all SCFA concentrations and favored SCFA consumption at 19 g/L SCFAs. The different SCFA uptake preferences in <i>C. curvatum</i> and <i>Y. lipolytica</i> highlighted the importance of considering the SCFA profile to select a suitable yeast strain for microbial oils production. At the most challenging SCFA concentration (19 g/L), 57.2% ±1.6% (w/w) and 78.4 ± 0.6% (w/w) lipid content were obtained in <i>C. curvatum</i> and <i>Y. lipolytica</i>, respectively. These values are among the highest reported for wild-type strains. To circumvent the challenges associated with media with high nitrogen content, this report also proved struvite precipitation as an effective method for increasing lipid production (from 17.9 ± 3.9% (w/w) to 41.9 ± 2.6% (w/w)) after nitrogen removal in food waste-derived media.</p>","PeriodicalId":8919,"journal":{"name":"Bioengineered","volume":"14 1","pages":"2286723"},"PeriodicalIF":4.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10761111/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138443693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-01Epub Date: 2023-10-18DOI: 10.1080/21655979.2023.2269349
{"title":"Statement of Retraction: The long non-coding RNA ASMTL-AS1 promotes hepatocellular carcinoma progression by sponging miR-1343-3p that suppresses LAMC1 (laminin subunit gamma 1).","authors":"","doi":"10.1080/21655979.2023.2269349","DOIUrl":"https://doi.org/10.1080/21655979.2023.2269349","url":null,"abstract":"","PeriodicalId":8919,"journal":{"name":"Bioengineered","volume":"14 1","pages":"2269349"},"PeriodicalIF":4.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10588517/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49673856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In the present era of global climate change, the scarcity of potable water is increasing both due to natural and anthropogenic causes. Water is the elixir of life, and its usage has risen significantly due to escalating economic activities, widespread urbanization, and industrialization. The increasing water scarcity and rising contamination have compelled, scientists and researchers, to adopt feasible and sustainable wastewater treatment methods in meeting the growing demand for freshwater. Presently, various waste treatment technologies are adopted across the globe, such as physical, chemical, and biological treatment processes. There is a need to replace these technologies with sustainable and green technology that encourages the use of microorganisms since they have proven to be more effective in water treatment processes. The present review article is focused on demonstrating how effectively various microbes can be used in wastewater treatment to achieve environmental sustainability and economic feasibility. The microbial consortium used for water treatment offers many advantages over pure culture. There is an urgent need to develop hybrid treatment technology for the effective remediation of various organic and inorganic pollutants from wastewater.
{"title":"Recent advances in microbial engineering approaches for wastewater treatment: a review.","authors":"Monika Sharma, Sangita Agarwal, Richa Agarwal Malik, Gaurav Kumar, Dan Bahadur Pal, Mamun Mandal, Abhijit Sarkar, Farkad Bantun, Shafiul Haque, Pardeep Singh, Neha Srivastava, Vijai Kumar Gupta","doi":"10.1080/21655979.2023.2184518","DOIUrl":"10.1080/21655979.2023.2184518","url":null,"abstract":"<p><p>In the present era of global climate change, the scarcity of potable water is increasing both due to natural and anthropogenic causes. Water is the elixir of life, and its usage has risen significantly due to escalating economic activities, widespread urbanization, and industrialization. The increasing water scarcity and rising contamination have compelled, scientists and researchers, to adopt feasible and sustainable wastewater treatment methods in meeting the growing demand for freshwater. Presently, various waste treatment technologies are adopted across the globe, such as physical, chemical, and biological treatment processes. There is a need to replace these technologies with sustainable and green technology that encourages the use of microorganisms since they have proven to be more effective in water treatment processes. The present review article is focused on demonstrating how effectively various microbes can be used in wastewater treatment to achieve environmental sustainability and economic feasibility. The microbial consortium used for water treatment offers many advantages over pure culture. There is an urgent need to develop hybrid treatment technology for the effective remediation of various organic and inorganic pollutants from wastewater.</p>","PeriodicalId":8919,"journal":{"name":"Bioengineered","volume":"14 1","pages":"2184518"},"PeriodicalIF":4.2,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10376923/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9892894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-01DOI: 10.1080/21655979.2023.2252234
Anisa Ratnasari
Membrane technology can play a suitable role in removing pharmaceutical active compounds since it requires low energy and simple operation. Even though membrane technology has progressed for wastewater applications nowadays, modifying membranes to achieve the strong desired membrane performance is still needed. Thus, this study overviews a comprehensive insight into the application of modified polymer membranes to remove pharmaceutical active compounds from wastewater. Biotoxicity of pharmaceutical active compounds is first prescribed to gain deep insight into how membranes can remove pharmaceutical active compounds from wastewater. Then, the behavior of the diffusion mechanism can be concisely determined using mass transfer factor model that represented by β and B with value up to 2.004 g h mg-1 and 1.833 mg g-1 for organic compounds including pharmaceutical active compounds. The model refers to the adsorption of solute to attach onto acceptor sites of the membrane surface, external mass transport of solute materials from the bulk liquid to the membrane surface, and internal mass transfer to diffuse a solute toward acceptor sites of the membrane surface with evidenced up to 0.999. Different pharmaceutical compounds have different solubility and relates to the membrane hydrophilicity properties and mechanisms. Ultimately, challenges and future recommendations have been presented to view the future need to enhance membrane performance regarding fouling mitigation and recovering compounds. Afterwards, the discussion of this study is projected to play a critical role in advance of better-quality membrane technologies for removing pharmaceutical active compounds from wastewater in an eco-friendly strategy and without damaging the ecosystem.
膜技术可以在去除药物活性化合物方面发挥适当的作用,因为它需要低能量和简单的操作。尽管如今膜技术在废水应用中取得了进展,但仍需要对膜进行改性以获得所需的强膜性能。因此,本研究综述了改性聚合物膜在去除废水中药物活性化合物方面的应用。药物活性化合物的生物毒性是为了深入了解膜如何从废水中去除药物活性化合物而首次提出的。然后,使用以β和B表示的传质因子模型,可以简明地确定扩散机制的行为,其值高达2.004 g h mg-1和1.833 mg g-1用于包括药物活性化合物的有机化合物。该模型指的是溶质吸附到膜表面的受体位点上,溶质材料从本体液体到膜表面上的外部质量传输,以及溶质向膜表面受体位点扩散的内部质量转移,证明高达0.999。不同的药物化合物具有不同的溶解度,并与膜的亲水性性质和机理有关。最终,提出了挑战和未来的建议,以考虑未来在减轻污染和回收化合物方面提高膜性能的需要。之后,本研究的讨论预计将在推进更高质量的膜技术方面发挥关键作用,以环保的策略从废水中去除药物活性化合物,而不会破坏生态系统。
{"title":"Modified polymer membranes for the removal of pharmaceutical active compounds in wastewater and its mechanism-A review.","authors":"Anisa Ratnasari","doi":"10.1080/21655979.2023.2252234","DOIUrl":"10.1080/21655979.2023.2252234","url":null,"abstract":"<p><p>Membrane technology can play a suitable role in removing pharmaceutical active compounds since it requires low energy and simple operation. Even though membrane technology has progressed for wastewater applications nowadays, modifying membranes to achieve the strong desired membrane performance is still needed. Thus, this study overviews a comprehensive insight into the application of modified polymer membranes to remove pharmaceutical active compounds from wastewater. Biotoxicity of pharmaceutical active compounds is first prescribed to gain deep insight into how membranes can remove pharmaceutical active compounds from wastewater. Then, the behavior of the diffusion mechanism can be concisely determined using mass transfer factor model that represented by β and B with value up to 2.004 g h mg<sup>-1</sup> and 1.833 mg g<sup>-1</sup> for organic compounds including pharmaceutical active compounds. The model refers to the adsorption of solute to attach onto acceptor sites of the membrane surface, external mass transport of solute materials from the bulk liquid to the membrane surface, and internal mass transfer to diffuse a solute toward acceptor sites of the membrane surface with evidenced up to 0.999. Different pharmaceutical compounds have different solubility and relates to the membrane hydrophilicity properties and mechanisms. Ultimately, challenges and future recommendations have been presented to view the future need to enhance membrane performance regarding fouling mitigation and recovering compounds. Afterwards, the discussion of this study is projected to play a critical role in advance of better-quality membrane technologies for removing pharmaceutical active compounds from wastewater in an eco-friendly strategy and without damaging the ecosystem.</p>","PeriodicalId":8919,"journal":{"name":"Bioengineered","volume":"14 1","pages":"2252234"},"PeriodicalIF":4.2,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ff/f6/KBIE_14_2252234.PMC10506444.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10308170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-01Epub Date: 2023-10-18DOI: 10.1080/21655979.2023.2269352
{"title":"Statement of Retraction: Circular RNA protein tyrosine kinase 2 (circPTK2) promotes colorectal cancer proliferation, migration, invasion and chemoresistance.","authors":"","doi":"10.1080/21655979.2023.2269352","DOIUrl":"https://doi.org/10.1080/21655979.2023.2269352","url":null,"abstract":"","PeriodicalId":8919,"journal":{"name":"Bioengineered","volume":"14 1","pages":"2269352"},"PeriodicalIF":4.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/93/73/KBIE_14_2269352.PMC10588533.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49673852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-01DOI: 10.1080/21655979.2023.2244235
Matthias Knödler, Katharina Frank, Lucy Kerpen, Johannes Felix Buyel
Antibody-drug conjugates (ADCs) can improve therapeutic indices compared to plain monoclonal antibodies (mAbs). However, ADC synthesis is complex because the components are produced separately in CHO cells (mAb) and often by chemical synthesis (drug). They are individually purified, coupled, and then the ADC is purified, increasing production costs compared to regular mAbs. In contrast, it is easier to produce recombinant fusion proteins consisting of an antibody derivative, linker and proteinaceous toxin, i.e. a recombinant immunotoxin (RIT). Plants are capable of the post-translational modifications needed for functional antibodies and can also express active protein toxins such as the recombinant mistletoe lectin viscumin, which is not possible in prokaryotes and mammalian cells respectively. Here, we used Nicotiana benthamiana and N. tabacum plants as well as tobacco BY-2 cell-based plant cell packs (PCPs) to produce effective RITs targeting CD64 as required for the treatment of myelomonocytic leukemia. We compared RITs with different subcellular targeting signals, linkers, and proteinaceous toxins. The accumulation of selected candidates was improved to ~ 40 mg kg-1 wet biomass using a design of experiments approach, and corresponding proteins were isolated with a purity of ~ 80% using an optimized affinity chromatography method with an overall yield of ~ 84%. One anti-CD64 targeted viscumin-based drug candidate was characterized in terms of storage stability and cytotoxicity test in vitro using human myelomonocytic leukemia cell lines. We identified bottlenecks in the plant-based expression platform that require further improvement and assessed critical process parameters that should be considered during process development for plant-made RITs.
{"title":"Design, optimization, production and activity testing of recombinant immunotoxins expressed in plants and plant cells for the treatment of monocytic leukemia.","authors":"Matthias Knödler, Katharina Frank, Lucy Kerpen, Johannes Felix Buyel","doi":"10.1080/21655979.2023.2244235","DOIUrl":"10.1080/21655979.2023.2244235","url":null,"abstract":"<p><p>Antibody-drug conjugates (ADCs) can improve therapeutic indices compared to plain monoclonal antibodies (mAbs). However, ADC synthesis is complex because the components are produced separately in CHO cells (mAb) and often by chemical synthesis (drug). They are individually purified, coupled, and then the ADC is purified, increasing production costs compared to regular mAbs. In contrast, it is easier to produce recombinant fusion proteins consisting of an antibody derivative, linker and proteinaceous toxin, i.e. a recombinant immunotoxin (RIT). Plants are capable of the post-translational modifications needed for functional antibodies and can also express active protein toxins such as the recombinant mistletoe lectin viscumin, which is not possible in prokaryotes and mammalian cells respectively. Here, we used <i>Nicotiana benthamiana</i> and <i>N. tabacum</i> plants as well as tobacco BY-2 cell-based plant cell packs (PCPs) to produce effective RITs targeting CD64 as required for the treatment of myelomonocytic leukemia. We compared RITs with different subcellular targeting signals, linkers, and proteinaceous toxins. The accumulation of selected candidates was improved to ~ 40 mg kg<sup>-1</sup> wet biomass using a design of experiments approach, and corresponding proteins were isolated with a purity of ~ 80% using an optimized affinity chromatography method with an overall yield of ~ 84%. One anti-CD64 targeted viscumin-based drug candidate was characterized in terms of storage stability and cytotoxicity test <i>in vitro</i> using human myelomonocytic leukemia cell lines. We identified bottlenecks in the plant-based expression platform that require further improvement and assessed critical process parameters that should be considered during process development for plant-made RITs.</p>","PeriodicalId":8919,"journal":{"name":"Bioengineered","volume":"14 1","pages":"2244235"},"PeriodicalIF":4.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/fd/75/KBIE_14_2244235.PMC10444015.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10413342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-01DOI: 10.1080/21655979.2023.2236842
Kevin Tian Xiang Tong, Inn Shi Tan, Henry Chee Yew Foo, Pau Loke Show, Man Kee Lam, Mee Kee Wong
The imminent need for transition to a circular biorefinery using microbial fuel cells (MFC), based on the valorization of renewable resources, will ameliorate the carbon footprint induced by industrialization. MFC catalyzed by bioelectrochemical process drew significant attention initially for its exceptional potential for integrated production of biochemicals and bioenergy. Nonetheless, the associated costly bioproduct production and slow microbial kinetics have constrained its commercialization. This review encompasses the potential and development of macroalgal biomass as a substrate in the MFC system for L-lactic acid (L-LA) and bioelectricity generation. Besides, an insight into the state-of-the-art technological advancement in the MFC system is also deliberated in detail. Investigations in recent years have shown that MFC developed with different anolyte enhances power density from several µW/m2 up to 8160 mW/m2. Further, this review provides a plausible picture of macroalgal-based L-LA and bioelectricity circular biorefinery in the MFC system for future research directions.
{"title":"Sustainable circular biorefinery approach for novel building blocks and bioenergy production from algae using microbial fuel cell.","authors":"Kevin Tian Xiang Tong, Inn Shi Tan, Henry Chee Yew Foo, Pau Loke Show, Man Kee Lam, Mee Kee Wong","doi":"10.1080/21655979.2023.2236842","DOIUrl":"10.1080/21655979.2023.2236842","url":null,"abstract":"<p><p>The imminent need for transition to a circular biorefinery using microbial fuel cells (MFC), based on the valorization of renewable resources, will ameliorate the carbon footprint induced by industrialization. MFC catalyzed by bioelectrochemical process drew significant attention initially for its exceptional potential for integrated production of biochemicals and bioenergy. Nonetheless, the associated costly bioproduct production and slow microbial kinetics have constrained its commercialization. This review encompasses the potential and development of macroalgal biomass as a substrate in the MFC system for L-lactic acid (L-LA) and bioelectricity generation. Besides, an insight into the state-of-the-art technological advancement in the MFC system is also deliberated in detail. Investigations in recent years have shown that MFC developed with different anolyte enhances power density from several µW/m<sup>2</sup> up to 8160 mW/m<sup>2</sup>. Further, this review provides a plausible picture of macroalgal-based L-LA and bioelectricity circular biorefinery in the MFC system for future research directions.</p>","PeriodicalId":8919,"journal":{"name":"Bioengineered","volume":"14 1","pages":"246-289"},"PeriodicalIF":4.2,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10367576/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9871760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-01Epub Date: 2023-11-29DOI: 10.1080/21655979.2023.2286722
Morgen Mukamwi, Tosin Somorin, Raimonda Soloha, Elina Dace
The world is facing problems of the increasing amount of resources wasted as the world population grows. Biowaste streams form a significant part of the overall waste generation, and a circular economy utilizing this biowaste will significantly reduce waste whilst lowering the anthropogenic carbon footprint. Due to their energy content and high concentration of hydrocarbon molecules, bio-based waste streams have the potential to be transformed into valorized products (energy, fuels, and chemicals) using biorefinery technologies. In this work, a mini-review has been conducted on available, mostly European databases on existing biomass types and biorefinery technologies to provide a framework for a desirable, comprehensive database connecting bio-based waste streams, biorefinery technologies and bioproducts, as well as the geographical distribution of feedstocks and biorefineries. The database assessment utilized the SWOT (strengths, weakness, opportunities, threats) methodology to support benchmark analysis and to identify critical gaps in underlying data structures that could be included in a single database. The results show that current databases are useful but insufficient for waste biorefineries due to limited quality and quantity as well as the usability of data. A comprehensive database or improved database cluster would be necessary, not only for technology development but for better investment and policy decisions. The development of the new database architecture would need to incorporate the aspects: expansion of database scope and content depth, improved usability, accessibility, applicability, update frequency, openness to new contributions, process descriptions and parameters, and technology readiness level.
{"title":"Databases for biomass and waste biorefinery - a mini-review and SWOT analysis.","authors":"Morgen Mukamwi, Tosin Somorin, Raimonda Soloha, Elina Dace","doi":"10.1080/21655979.2023.2286722","DOIUrl":"10.1080/21655979.2023.2286722","url":null,"abstract":"<p><p>The world is facing problems of the increasing amount of resources wasted as the world population grows. Biowaste streams form a significant part of the overall waste generation, and a circular economy utilizing this biowaste will significantly reduce waste whilst lowering the anthropogenic carbon footprint. Due to their energy content and high concentration of hydrocarbon molecules, bio-based waste streams have the potential to be transformed into valorized products (energy, fuels, and chemicals) using biorefinery technologies. In this work, a mini-review has been conducted on available, mostly European databases on existing biomass types and biorefinery technologies to provide a framework for a desirable, comprehensive database connecting bio-based waste streams, biorefinery technologies and bioproducts, as well as the geographical distribution of feedstocks and biorefineries. The database assessment utilized the SWOT (strengths, weakness, opportunities, threats) methodology to support benchmark analysis and to identify critical gaps in underlying data structures that could be included in a single database. The results show that current databases are useful but insufficient for waste biorefineries due to limited quality and quantity as well as the usability of data. A comprehensive database or improved database cluster would be necessary, not only for technology development but for better investment and policy decisions. The development of the new database architecture would need to incorporate the aspects: expansion of database scope and content depth, improved usability, accessibility, applicability, update frequency, openness to new contributions, process descriptions and parameters, and technology readiness level.</p>","PeriodicalId":8919,"journal":{"name":"Bioengineered","volume":"14 1","pages":"2286722"},"PeriodicalIF":4.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10761086/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138450793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-01Epub Date: 2023-11-29DOI: 10.1080/21655979.2023.2288354
Naouar Ben Ali, Rajae Benkaddour, Safaa Rahmouni, Ouafaa Hamdoun, Ibtissam Boussaoudi, Mustapha Hassoun, Latifa Azaroual, Alain Badoc, Patrick Martin, Ahmed Lamarti
Quercus suber L. is the main woody tree species in the Mediterranean basin. The in vitro regeneration from adult material, through primary somatic embryogenesis, is a well-known process, but the use of secondary somatic embryos for plant regeneration remains a very sparsely studied process. The main objective of this work is to explore the cork oak regeneration potential by using the secondary somatic embryogenesis process. Mainly, in this work, we report the polyamine effect. Explants used consisted on primary mature embryos, derived from leaves rejuvenated by epicormic shoot of the Moroccan Quercus suber. Three different polyamines were added to the basal medium, which was composed by macronutrients of N30K, 30 g/l glucose, and 7 g/l agar. Three polyamines, Putrescine, Spermine, and Spermidine, were added to the basal medium at 0.1, 0.2, 0.3, 0.4, 0.5, and 0.6 mg/l. Explants were tested after 8 weeks. Morphological analysis showed that the medium with 0.4 mg/l Spermidine provided the best result for secondary embryos, which corresponds to a very significant (p < 0.05) increase of 375%. The number of secondary embryos directly formed was 2.70 ± 0.51. Similarly, the optimum concentrations for high number of clusters (0.50 ± 0.11) and embryo clusters (1.43 ± 0.35) were increased by 145% and 158%. The addition of the polyamine also acted on the quality of embryos formed. A very significant (p < 0.05) increase in the size of secondary embryos was observed compared to the medium without polyamines. Spermidine showed the greatest increase (about 38%).
{"title":"Influence of exogenous polyamines on the secondary somatic embryogenesis of cork oak (<i>Quercus suber</i> L.).","authors":"Naouar Ben Ali, Rajae Benkaddour, Safaa Rahmouni, Ouafaa Hamdoun, Ibtissam Boussaoudi, Mustapha Hassoun, Latifa Azaroual, Alain Badoc, Patrick Martin, Ahmed Lamarti","doi":"10.1080/21655979.2023.2288354","DOIUrl":"10.1080/21655979.2023.2288354","url":null,"abstract":"<p><p><i>Quercus suber</i> L. is the main woody tree species in the Mediterranean basin. The <i>in vitro</i> regeneration from adult material, through primary somatic embryogenesis, is a well-known process, but the use of secondary somatic embryos for plant regeneration remains a very sparsely studied process. The main objective of this work is to explore the cork oak regeneration potential by using the secondary somatic embryogenesis process. Mainly, in this work, we report the polyamine effect. Explants used consisted on primary mature embryos, derived from leaves rejuvenated by epicormic shoot of the Moroccan <i>Quercus suber</i>. Three different polyamines were added to the basal medium, which was composed by macronutrients of N<sub><b>30</b></sub>K, 30 g/l glucose, and 7 g/l agar. Three polyamines, Putrescine, Spermine, and Spermidine, were added to the basal medium at 0.1, 0.2, 0.3, 0.4, 0.5, and 0.6 mg/l. Explants were tested after 8 weeks. Morphological analysis showed that the medium with 0.4 mg/l Spermidine provided the best result for secondary embryos, which corresponds to a very significant (<i>p</i> < 0.05) increase of 375%. The number of secondary embryos directly formed was 2.70 ± 0.51. Similarly, the optimum concentrations for high number of clusters (0.50 ± 0.11) and embryo clusters (1.43 ± 0.35) were increased by 145% and 158%. The addition of the polyamine also acted on the quality of embryos formed. A very significant (<i>p</i> < 0.05) increase in the size of secondary embryos was observed compared to the medium without polyamines. Spermidine showed the greatest increase (about 38%).</p>","PeriodicalId":8919,"journal":{"name":"Bioengineered","volume":"14 1","pages":"2288354"},"PeriodicalIF":4.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10761026/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138457653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-01Epub Date: 2023-10-18DOI: 10.1080/21655979.2023.2269351
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