Biomedical Journal最新文献_第4页

Navigating AI in cardiology: A scoping review of integration through clinical decision support systems for acute coronary syndrome 在心脏病学中导航人工智能：急性冠状动脉综合征临床决策支持系统整合的范围审查。

IF 4.4 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomedical Journal

Pub Date : 2025-10-01 DOI: 10.1016/j.bj.2025.100853

Shu-Hui Chen , Chin-Chieh Wu , Kuan-Fu Chen

Background

The integration of AI in diagnosing and managing ACS shows increasing promise, yet challenges remain in translating AI-CDSS into clinical practice. This study evaluates the advancements and limitations of AI for ACS over the past three years, purpose of understanding the scope, limitations, and potential of AI-CDSS in ACS.

Materials and methods

We conducted a systematic review of recent literature, adhering to guidelines for systematic reviews. We applied QUADAS-2 and PROBAST tools for quality assessment, focusing on biases in study designs. Ten studies about AI-CDSS in ACS management underwent critical analysis, emphasizing the strength of their research methods and the thoroughness of their prospective validation to ensure theoretical integrity and practical reliability.

Results

Our research reveals that while discourse around AI-CDSS in ACS management intensifies, obstacles hinder efficacy in practical settings. These challenges include biases in tests and unrepresentative patient selection, pointing to the need for rigorous and inclusive samples. The lack of sufficient external and prospective validation in studies also raises concerns clinical utility of AI-CDSS. The result is the gap between the potential benefits of AI-CDSS and the actual impact of improving diagnostic accuracy and outcomes for ACS limitations identified.

Conclusions

While AI-CDSS shows promise for improving diagnostic accuracy, treatment efficacy, and workflows in ACS, this study highlights the imperative to enhance model validation, including prospective validation, and address lingering diagnostic gaps. Improving study design and mitigating biases remain crucial for the acceptance and effectiveness of AI-CDSS in acute cardiac care settings.

背景：人工智能在ACS诊断和管理中的整合显示出越来越大的希望，但在将人工智能- cdss转化为临床实践方面仍然存在挑战。本研究评估了过去三年来AI在ACS中的进展和局限性，目的是了解AI- cdss在ACS中的范围、局限性和潜力。材料和方法：我们对最近的文献进行了系统的综述，并遵循了系统综述的指南。我们应用QUADAS-2和PROBAST工具进行质量评估，重点关注研究设计中的偏差。对10项关于AI-CDSS在ACS管理中的研究进行了批判性分析，强调其研究方法的强度和前瞻性验证的彻彻性，以确保理论的完整性和实践的可靠性。结果：我们的研究表明，虽然围绕AI-CDSS在ACS管理中的讨论越来越多，但在实际环境中，障碍阻碍了疗效。这些挑战包括测试中的偏差和不具代表性的患者选择，这表明需要严格和包容的样本。研究中缺乏足够的外部和前瞻性验证也引起了对AI-CDSS临床应用的关注。结果是AI-CDSS的潜在益处与提高ACS局限性诊断准确性和结果的实际影响之间的差距。结论：虽然AI-CDSS有望提高ACS的诊断准确性、治疗疗效和工作流程，但本研究强调了加强模型验证的必要性，包括前瞻性验证，并解决遗留的诊断差距。改进研究设计和减轻偏倚对于急性心脏护理环境中AI-CDSS的接受度和有效性仍然至关重要。

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引用次数: 0

Unlocking the potential of repetitive transcranial magnetic stimulation to enhance motor function in pediatric cerebral palsy: A comprehensive review 释放重复经颅磁刺激增强小儿脑瘫运动功能的潜力：一项综合综述。

IF 4.4 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomedical Journal

Pub Date : 2025-10-01 DOI: 10.1016/j.bj.2025.100835

Chia-Ling Chen

Background

Cerebral Palsy (CP) poses a substantial challenge to pediatric motor function, necessitating effective rehabilitative interventions. This review focuses on the potential of Repetitive Transcranial Magnetic Stimulation (rTMS) as a therapeutic approach for pediatric CP. The context and purpose are framed within the need for novel strategies to enhance motor function in affected children.

Material and methods

The study scrutinizes existing literature to assess the efficacy of rTMS in pediatric CP. Methodological details, including stimulation protocols, are explored. Statistical tests employed in the reviewed studies are outlined, providing insight into the scientific rigor applied in evaluating rTMS outcomes.

Results

The main findings from the literature review highlight the positive impact of rTMS on motor function and spasticity reduction in children with CP. The synergistic effects observed when combining rTMS with conventional therapies such as physical or occupational therapy and constraint-induced movement therapy are emphasized.

Conclusions

Evidences from literature review affirm that rTMS yields constructive outcomes, encompassing enhanced motor function and diminished spasticity, especially when combined with therapies like physical or occupational therapy, and constraint-induced movement therapy. Nevertheless, optimizing rTMS necessitates further fine-tuning of stimulation parameters. Ethical considerations and adherence to safety guidelines are crucial in pediatric settings, despite rare and typically benign side effects. Future research should focus on larger samples, stringent research designs, and long-term follow-ups to rTMS as an effective therapy for managing motor impediments in children with CP.

背景：脑瘫（CP）对儿童运动功能造成了巨大的挑战，需要有效的康复干预。这篇综述的重点是重复经颅磁刺激（rTMS）作为儿童CP治疗方法的潜力。背景和目的是在需要新的策略来增强受影响儿童的运动功能的框架内。材料和方法：本研究仔细审查了现有文献，以评估rTMS在儿科CP中的疗效。方法细节，包括刺激方案，进行了探讨。概述了所审查的研究中采用的统计测试，提供了对评估rTMS结果所应用的科学严谨性的见解。结果：文献综述的主要发现强调了rTMS对CP儿童运动功能和痉挛减轻的积极影响，并强调了rTMS与常规治疗（如物理或职业治疗和约束诱导运动治疗）联合使用时观察到的协同效应。结论：来自文献综述的证据证实，rTMS产生了建设性的结果，包括增强运动功能和减少痉挛，特别是当与物理或职业治疗以及约束诱导运动治疗等治疗相结合时。然而，优化rTMS需要进一步微调增产参数。尽管有罕见且典型的良性副作用，但在儿科环境中，伦理考虑和遵守安全指南至关重要。未来的研究应该集中在更大的样本，严格的研究设计，并长期随访rTMS作为一种有效的治疗CP儿童运动障碍的方法。

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引用次数: 0

Toward a personalized chronotherapy of blood pressure 迈向个性化的血压时间疗法。

IF 4.4 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomedical Journal

Pub Date : 2025-10-01 DOI: 10.1016/j.bj.2025.100849

Germaine Cornelissen , Yoshihiko Watanabe , Larry A. Beaty , Kuniaki Otsuka

Background

A consensus regarding the optimal time to administer anti-hypertensive medications has not been reached. Possible differential effects of different anti-hypertensive drugs on the circadian pattern of blood pressure (BP) and differential responses of individual patients receiving the same treatment at different times of the day may partly account for the controversy.

Methods

Ambulatory blood pressure monitoring (ABPM) data available at 30-min intervals for 7 days from previous studies are reanalyzed to compare the effect of five drugs (amlodipine, atenolol, captopril retard, long-acting carteolol, and nilvadipine) taken 1.5 h after awakening or twice a day on the 24-h profile of BP from 7 to 13 conventionally diagnosed patients per treatment group. Similar data from 30 patients receiving losartan/hydrochlorothiazide for at least one month at each of six different times in relation to their time of awakening serve to compare the effect of treatment time in different patients.

Results

Some but not all drugs affected the 24-h amplitude and/or phase of BP or the contribution of the 12-h harmonic term to modify the circadian waveform of BP. While evening dosing increased the 24-h amplitude of BP in some patients, other patients achieved such a desired effect with morning dosing.

Conclusion

Personalized optimization of treatment timing to best match a healthy circadian BP pattern is recommended, guided by chronobiological analyses of ABPM data collected over several days in view of the large day-to-day variability in all features of the 24-h BP rhythm.

背景：关于服用抗高血压药物的最佳时间尚未达成共识。不同的降压药对血压昼夜节律模式的不同影响，以及在一天中不同时间接受相同治疗的个体患者的不同反应，可能是引起争议的部分原因。方法：重新分析以往研究中每隔7天30分钟的动态血压监测（ABPM）数据，比较5种药物（氨氯地平、阿替洛尔、卡托普利缓释片、长效卡替洛尔和尼伐地平）在醒后1.5小时或每天2次服用对每组7 ~ 13例常规诊断患者24小时血压的影响。来自30名接受氯沙坦/氢氯噻嗪治疗至少1个月的患者的类似数据，在6个不同的时间中，每个时间与他们的觉醒时间有关，用于比较治疗时间对不同患者的影响。结果：部分但非全部药物影响血压的24小时振幅和/或相位，或影响12小时谐波项对血压昼夜波形的贡献。虽然晚间给药增加了一些患者的24小时血压振幅，但其他患者在早晨给药时达到了预期的效果。结论：考虑到24小时血压节律的所有特征每天都有很大的变化，建议在对几天内收集的ABPM数据进行时间生物学分析的指导下，个性化优化治疗时机，以最佳地匹配健康的昼夜节律模式。

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引用次数: 0

Linking mitochondrial dysfunction, hormonal decline, and aging to cancer development 将线粒体功能障碍、激素下降和衰老与癌症发展联系起来。

IF 4.4 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomedical Journal

Pub Date : 2025-10-01 DOI: 10.1016/j.bj.2025.100887

Yu-Hsiang Lin , Kuo-Jen Lin , Jau-Yuan Chen

引用次数: 0

Integrating comprehensive genomic profiling in the management of oncology patients: Applications and challenges in Taiwan 整合全面基因组分析在肿瘤患者管理：台湾的应用与挑战。

IF 4.4 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomedical Journal

Pub Date : 2025-10-01 DOI: 10.1016/j.bj.2025.100851

Chen-Yang Huang , Wen-Kuan Huang , Kun-Yun Yeh , John Wen-Cheng Chang , Yung-Chang Lin , Wen-Chi Chou

Comprehensive genomic profiling (CGP) refers to the detailed genomic analysis of cancers for oncology patients. With the rapid development of next-generation sequencing (NGS) technologies, CGP has been widely applied to clinical practice and managing oncology patients. CGP can be performed on the tumor DNA and RNA, as well as non-tumor tissues (e.g., blood, pleural effusion, and ascites). In this article, we review the current evidence supporting the use of CGP in the management of oncology patients, both in real-world practice and the bridging to clinical trials. We also discuss the role of the molecular tumor board on the application of CGP in oncology patients. We provide an overview of the current scheme of CGP reimbursement in Taiwan and the precision oncology branch of the National Biobank Consortium of Taiwan. Finally, we discuss about the potential barriers and challenges of applying CGP in managing oncology patients and the future perspectives of CGP in precision oncology.

综合基因组分析（Comprehensive genomic profiling， CGP）是指对肿瘤患者进行详细的癌症基因组分析。随着新一代测序（NGS）技术的快速发展，CGP已广泛应用于临床实践和肿瘤患者管理。CGP可用于肿瘤DNA和RNA，也可用于非肿瘤组织（如血液、胸腔积液、腹水）。在这篇文章中，我们回顾了目前支持在肿瘤患者管理中使用CGP的证据，无论是在现实世界的实践中还是在临床试验中。我们还讨论了分子肿瘤委员会对CGP在肿瘤患者中的应用的作用。最后，我们讨论了在肿瘤患者管理中应用CGP的潜在障碍和挑战，以及CGP在精准肿瘤学中的未来前景。

引用次数: 0

Sleep, interrupted – when short nights take their toll 睡眠，被打断——当短暂的夜晚让你付出代价。

IF 4.4 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomedical Journal

Pub Date : 2025-10-01 DOI: 10.1016/j.bj.2025.100915

Aila Akosua Kattner

Sleep loss is increasingly recognized as a contributor to neuropathic pain, while diagnostic accuracy in thyroid carcinoma may be improved through refined assessment methods. In pediatric patients with cerebral palsy, transcranial magnetic stimulation (TMS) shows promise as a therapeutic intervention. Chronotherapy of blood pressure demonstrates how aligning antihypertensive treatment with circadian patterns may enhance efficacy. Artificial intelligence–based clinical decision support systems offer potential in the management of acute coronary syndromes, although current limitations and risks require careful consideration. Advances in precision oncology include comprehensive genomic profiling and spatial omics, while large language models highlight both opportunities and challenges in healthcare applications. Finally, age-related hormonal changes in combination with mitochondrial dysfunction are discussed in the context of carcinogenesis, underscoring the complex links between aging, metabolism, and cancer development.

睡眠不足越来越被认为是神经性疼痛的一个因素，而甲状腺癌的诊断准确性可以通过改进的评估方法来提高。在小儿脑瘫患者中，经颅磁刺激（TMS）显示出作为一种治疗干预的希望。血压的时间疗法表明如何调整抗高血压治疗与昼夜节律模式可能提高疗效。基于人工智能的临床决策支持系统为急性冠状动脉综合征的管理提供了潜力，尽管目前的局限性和风险需要仔细考虑。精确肿瘤学的进展包括全面的基因组分析和空间组学，而大型语言模型突出了医疗保健应用中的机遇和挑战。最后，在致癌的背景下讨论了与年龄相关的激素变化与线粒体功能障碍的结合，强调了衰老、代谢和癌症发展之间的复杂联系。

引用次数: 0

Correlation of SALL1 with CEUS parameters and immune escape in thyroid carcinoma 甲状腺癌中SALL1与超声造影参数及免疫逃逸的相关性

IF 4.4 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomedical Journal

Pub Date : 2025-10-01 DOI: 10.1016/j.bj.2025.100829

Xia Li, Shuang Xu, Liuwei Hao, Xiaoning Zhou

Background

Contrast-enhanced ultrasonography (CEUS) is widely used to diagnose thyroid carcinoma (TC), though its accuracy in differentiating malignant nodules is limited. We identified TC-associated differentially expressed genes (DEGs) and examined the impact of these genes, particularly SALL1, on immune escape mechanisms within TC cells.

Methods and materials

DEG analysis was conducted on GSE65144 dataset to identify genes associated with TC. Functional enrichment analysis focused on genes related to pituitary function, with SALL1 identified as a key candidate. Clinical data from TC cases were used to assess the diagnostic impact of combining SALL1 expression with CEUS parameters. Additionally, TCP-1 cell lines with manipulated SALL1 expression were used to investigate cellular behaviors in vitro, while in vivo studies in nude mice evaluated tumor growth and immune microenvironment changes linked to SALL1 expression.

Results

We identified 152 DEGs, including NKX2-1, CDH1, and SALL1, which are associated with pituitary function. TIMER database analysis revealed SALL1's correlation with immune cell infiltration in TC. Clinically, SALL1 was downregulated in TC and showed a significant correlation with CEUS parameters, and combining SALL1 expression with CEUS markedly enhanced diagnostic accuracy for TC. In vitro, low SALL1 expression increased cell proliferation, TC progression, and immune escape, whereas upregulation led to reduced cell activity, increased apoptosis, and activation of autophagy and pyroptosis. In vivo, nude mouse models demonstrated that silencing SALL1 enhanced tumor growth, while overexpression inhibited tumor progression and modulated immune microenvironment.

Conclusions

Combining SALL1 with CEUS improves TC diagnostic accuracy, highlighting SALL1 as a potential biomarker in TC.

背景：超声造影（CEUS）广泛用于诊断甲状腺癌（TC），但其鉴别恶性结节的准确性有限。我们鉴定了TC相关的差异表达基因（DEGs），并研究了这些基因，特别是SALL1对TC细胞内免疫逃逸机制的影响。方法与材料：对GSE65144数据集进行DEG分析，鉴定与TC相关的基因。功能富集分析侧重于与垂体功能相关的基因，其中SALL1被确定为关键候选基因。使用TC病例的临床数据来评估SALL1表达与超声造影参数联合诊断的影响。此外，使用操纵SALL1表达的TCP-1细胞系研究体外细胞行为，而在裸鼠体内研究评估与SALL1表达相关的肿瘤生长和免疫微环境变化。结果：我们鉴定出152个与垂体功能相关的基因，包括NKX2-1、CDH1和SALL1。TIMER数据库分析显示SALL1与TC免疫细胞浸润相关。临床发现，SALL1在TC中表达下调，且与CEUS参数有显著相关性，将SALL1表达与CEUS结合可显著提高TC的诊断准确性。在体外，低SALL1表达增加细胞增殖、TC进展和免疫逃逸，而上调则导致细胞活性降低、细胞凋亡增加、自噬和焦亡活化。在体内，裸鼠模型显示沉默SALL1可促进肿瘤生长，而过表达SALL1可抑制肿瘤进展并调节免疫微环境。结论：SALL1联合超声造影可提高TC的诊断准确性，突出SALL1作为TC的潜在生物标志物。

{"title":"Correlation of SALL1 with CEUS parameters and immune escape in thyroid carcinoma","authors":"Xia Li, Shuang Xu, Liuwei Hao, Xiaoning Zhou","doi":"10.1016/j.bj.2025.100829","DOIUrl":"10.1016/j.bj.2025.100829","url":null,"abstract":"<div><h3>Background</h3><div>Contrast-enhanced ultrasonography (CEUS) is widely used to diagnose thyroid carcinoma (TC), though its accuracy in differentiating malignant nodules is limited. We identified TC-associated differentially expressed genes (DEGs) and examined the impact of these genes, particularly SALL1, on immune escape mechanisms within TC cells.</div></div><div><h3>Methods and materials</h3><div>DEG analysis was conducted on GSE65144 dataset to identify genes associated with TC. Functional enrichment analysis focused on genes related to pituitary function, with SALL1 identified as a key candidate. Clinical data from TC cases were used to assess the diagnostic impact of combining SALL1 expression with CEUS parameters. Additionally, TCP-1 cell lines with manipulated SALL1 expression were used to investigate cellular behaviors <em>in vitro</em>, while <em>in vivo</em> studies in nude mice evaluated tumor growth and immune microenvironment changes linked to SALL1 expression.</div></div><div><h3>Results</h3><div>We identified 152 DEGs, including NKX2-1, CDH1, and SALL1, which are associated with pituitary function. TIMER database analysis revealed SALL1's correlation with immune cell infiltration in TC. Clinically, SALL1 was downregulated in TC and showed a significant correlation with CEUS parameters, and combining SALL1 expression with CEUS markedly enhanced diagnostic accuracy for TC. <em>In vitro</em>, low SALL1 expression increased cell proliferation, TC progression, and immune escape, whereas upregulation led to reduced cell activity, increased apoptosis, and activation of autophagy and pyroptosis. <em>In vivo</em>, nude mouse models demonstrated that silencing SALL1 enhanced tumor growth, while overexpression inhibited tumor progression and modulated immune microenvironment.</div></div><div><h3>Conclusions</h3><div>Combining SALL1 with CEUS improves TC diagnostic accuracy, highlighting SALL1 as a potential biomarker in TC.</div></div>","PeriodicalId":8934,"journal":{"name":"Biomedical Journal","volume":"48 5","pages":"Article 100829"},"PeriodicalIF":4.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145306835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Roles and potential of Large language models in healthcare: A comprehensive review 大型语言模型在医疗保健中的作用和潜力：一个全面的综述。

IF 4.4 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomedical Journal

Pub Date : 2025-10-01 DOI: 10.1016/j.bj.2025.100868

Chihung Lin , Chang-Fu Kuo

Large Language Models (LLMs) are capable of transforming healthcare by demonstrating remarkable capabilities in language understanding and generation. They have matched or surpassed human performance in standardized medical examinations and assisted in diagnostics across specialties like dermatology, radiology, and ophthalmology. LLMs can enhance patient education by providing accurate, readable, and empathetic responses, and they can streamline clinical workflows through efficient information extraction from unstructured data such as clinical notes. Integrating LLM into clinical practice involves user interface design, clinician training, and effective collaboration between Artificial Intelligence (AI) systems and healthcare professionals. Users must possess a solid understanding of generative AI and domain knowledge to assess the generated content critically. Ethical considerations to ensure patient privacy, data security, mitigating biases, and maintaining transparency are critical for responsible deployment. Future directions for LLMs in healthcare include interdisciplinary collaboration, developing new benchmarks that incorporate safety and ethical measures, advancing multimodal LLMs that integrate text and imaging data, creating LLM-based medical agents capable of complex decision-making, addressing underrepresented specialties like rare diseases, and integrating LLMs with robotic systems to enhance precision in procedures. Emphasizing patient safety, ethical integrity, and human-centered implementation is essential for maximizing the benefits of LLMs, while mitigating potential risks, thereby helping to ensure that these AI tools enhance rather than replace human expertise and compassion in healthcare.

大型语言模型（llm）能够通过展示语言理解和生成方面的卓越能力来改变医疗保健。它们在标准化医学检查方面的表现已经达到或超过了人类，并协助皮肤科、放射科和眼科等专业的诊断。法学硕士可以通过提供准确、可读和共情的回应来加强患者教育，他们可以通过从临床记录等非结构化数据中高效地提取信息来简化临床工作流程。将LLM整合到临床实践中涉及用户界面设计，临床医生培训以及人工智能（AI）系统和医疗保健专业人员之间的有效协作。用户必须对生成式人工智能和领域知识有扎实的理解，才能批判性地评估生成的内容。确保患者隐私、数据安全、减轻偏见和保持透明度的伦理考虑对于负责任的部署至关重要。法学硕士在医疗保健领域的未来发展方向包括跨学科合作，开发包含安全和伦理措施的新基准，推进整合文本和图像数据的多模式法学硕士，创建能够进行复杂决策的基于法学硕士的医疗代理，解决罕见疾病等代表性不足的专业，以及将法学硕士与机器人系统集成以提高程序的准确性。强调患者安全、道德诚信和以人为本的实施对于llm的利益最大化至关重要，同时降低潜在风险，从而有助于确保这些人工智能工具增强而不是取代医疗保健领域的人类专业知识和同情心。

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引用次数: 0

Long Non-Coding RNAs as Modulators of Metabolic Reprogramming for Endogenous Heart Regeneration: Mechanisms and Therapeutic Potential. 长链非编码rna作为内源性心脏再生代谢重编程的调节剂：机制和治疗潜力。

IF 4.4 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomedical Journal

Pub Date : 2025-09-15 DOI: 10.1016/j.bj.2025.100914

Xueping Wu, Yehui Lv, Zhihong Li, Zhifang Yang

Myocardial infarction (MI) is one of the leading causes of death worldwide, with its high incidence and mortality posing a significant threat to human health. Despite some progress in the treatment of myocardial infarction, mortality rates remain alarmingly high. Adult mammals have limited myocardial regenerative capacity, and extensive cell death caused by myocardial ischemia severely impairs cardiac function, leading to heart failure or death. In contrast, neonatal myocardium possesses a robust regenerative ability, which gradually diminishes after birth. The loss of cardiomyocyte regenerative capacity is often accompanied by a shift in energy metabolism-from reliance on glucose (glycolysis) to fatty acid oxidation. This metabolic reprogramming significantly impacts CM proliferation. Long non-coding RNAs (lncRNAs) orchestrate cardiac regeneration through epigenetic control (e.g., Bvht/PRC2-mediated silencing), metabolic reprogramming (e.g., GATA6-AS1 suppression of FAO), and miRNA sponging (e.g., CAREL sequestration of miR-296). However, our understanding of the metabolic determinants and pathways that promote myocardial regeneration after myocardial infarction is still insufficient. This review investigates the interplay between lncRNAs and metabolic reprogramming in cardiovascular function, aiming to identify novel therapeutic targets and strategies to enhance myocardial regeneration post-MI.

心肌梗死（MI）是世界范围内导致死亡的主要原因之一，其高发病率和死亡率对人类健康构成重大威胁。尽管在治疗心肌梗塞方面取得了一些进展，但死亡率仍然高得惊人。成年哺乳动物的心肌再生能力有限，心肌缺血引起的广泛细胞死亡严重损害心功能，导致心力衰竭或死亡。相反，新生儿心肌具有强大的再生能力，但在出生后逐渐减弱。心肌细胞再生能力的丧失通常伴随着能量代谢的转变——从依赖葡萄糖（糖酵解）到脂肪酸氧化。这种代谢重编程显著影响CM增殖。长链非编码rna （lncRNAs）通过表观遗传控制（如Bvht/ prc2介导的沉默）、代谢重编程（如GATA6-AS1对FAO的抑制）和miRNA海绵（如miR-296的CAREL隔离）来协调心脏再生。然而，我们对心肌梗死后促进心肌再生的代谢决定因素和途径的理解仍然不足。本文综述了lncrna与心血管功能代谢重编程之间的相互作用，旨在寻找新的治疗靶点和策略来增强心肌梗死后的心肌再生。

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引用次数: 0

Neuropeptide FF Receptor 2 Overexpression Aggravates Lipid Accumulation and Metabolic Dysfunction in Mice with Diet-Induced Metabolic Stress. 神经肽FF受体2过表达加重饮食诱导代谢应激小鼠的脂质积累和代谢功能障碍。

IF 4.4 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomedical Journal

Pub Date : 2025-09-12 DOI: 10.1016/j.bj.2025.100913

Hsiang-Ting Hsu, Chun-Chun Hsu, Yun-Jou Liao, Hui-Yun Li, Yao-Chang Chiang, Ya-Tin Lin

Background: Obesity is a major contributor to metabolic dysfunction and is driven by complex genetic, behavioral, and physiological factors. Neuropeptide FF receptor 2 (NPFFR2) has been implicated in regulating feeding behavior, as well as energy and glucose homeostasis. However, its precise role in obesity and metabolic disorders remains unclear. This study aimed to investigate the systemic role of NPFFR2 in obesity-induced metabolic dysfunction.

Material and methods: The role of NPFFR2 was examined using wild-type and Npffr2-overexpressing transgenic mice subjected to 15 weeks of high-fat high-sucrose diet to induce obesity. Systemic, tissue-specific, and serum metabolic profiles were analyzed, with a particular focus on lipid abnormalities in the liver and adipose tissues.

Results: Npffr2 overexpression exacerbated obesity-induced metabolic dysfunction, including accelerated body weight gain, impaired glucose homeostasis, altered fat composition, adipose tissue inflammation, and dysregulated lipid metabolism. In addition, hypertrophy of both hepatocytes and adipocytes was aggravated in Npffr2-overexpressing mice, collectively contributing to excessive energy storage and reduced metabolic efficiency.

Conclusions: These findings suggest that NPFFR2 may contribute to the regulation of energy balance and lipid metabolism, potentially via central regulatory pathways. These findings highlight the need for mechanistic studies to clarify its region-specific roles and therapeutic potential in metabolic disorders.

背景：肥胖是代谢功能障碍的主要原因，受复杂的遗传、行为和生理因素的影响。神经肽FF受体2 （NPFFR2）参与调节摄食行为以及能量和葡萄糖稳态。然而，它在肥胖和代谢紊乱中的确切作用尚不清楚。本研究旨在探讨NPFFR2在肥胖诱导的代谢功能障碍中的全系统作用。材料和方法：采用野生型和过表达NPFFR2的转基因小鼠，经15周高脂高糖饮食诱导肥胖，检测NPFFR2的作用。分析了全身、组织特异性和血清代谢谱，特别关注肝脏和脂肪组织中的脂质异常。结果：Npffr2过表达加剧了肥胖诱导的代谢功能障碍，包括体重增加加速、葡萄糖稳态受损、脂肪成分改变、脂肪组织炎症和脂质代谢失调。此外，npffr2过表达小鼠的肝细胞和脂肪细胞肥大加剧，共同导致能量储存过度和代谢效率降低。结论：这些发现表明NPFFR2可能通过中枢调控途径参与能量平衡和脂质代谢的调节。这些发现强调需要进行机制研究，以阐明其在代谢紊乱中的区域特异性作用和治疗潜力。

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引用次数: 0