Biofizika最新文献

The paper presents the idea of transparent evolution through the long-term reaction of the planet Earth on the external flow of radiant energy from the Sun. Due to limitations of matter on Earth, as well as on any other planet, the continuous pumping flow of radiant energy was shown to lead to cyclization and transport of substance on emerging gradients. The evolution of energy-matter interaction follows the path of capturing and transferring more energy by the fewer matter, i.e., the path of growth of the amount of energy used by each unit mass. For this indicator, the least effective mass transfer is a simple mass transfer as vortices of gases, in the gradients of temperature and pressure, which occurred on the primary surface of the planet. A long-term natural selection related to the accumulation of water on the planet has played a special role in developing the interaction of energy and matter. Phase transformations (ice, water, vapor) and mechanical transfers are the most common energy-matter processes. Based on water cycles, cyclic transports and transformations, chemical transformation of substances became possible developing over time into a biological transformation. This kind of the interaction of energy and matter is most efficient. In particular, during photosynthesis the energy of our star "is captured and utilized" in the most active part of the spectrum of its radiation. In the process of biological evolution of heterotrophs, a rise (by a factor of hundreds) in the coefficient that characterizes the intensity of energy exchange from protozoa to mammals is most illustratory. The development and the current dominance of humans as the most energy-using active species in capturing the energy and meaningful organization of its new flows especially on the basis of organic debris of former biospheres is admirable, but quite natural from the energy positions. In the course of technological evolution of humankind, the measure of the intensity of energy for homoeothermic (warm-blooded) animals has increased 20 times, based on the process energy used by the "average" inhabitant of the world. Thus, the victory of our species in planetary evolution is easy to fit into the mainstream of evolution through energy-matter interactions: multiple growth of star energy was used to transform the matter on the surface of the irradiated planet.

Results of local fractal analysis of 329-per-day time series of 239Pu alpha-decay rate fluctuations by means of all permutations method (APM) are presented. The APM-analysis reveals in the time series some steady frequency set. The coincidence of the frequency set with the Earth natural oscillations was demonstrated. A short review of works by different authors who analyzed the time series of fluctuations in processes of different nature is given. We have shown that the periods observed in those works correspond to the periods revealed in our study. It points to a common mechanism of the phenomenon observed.

The effect of water isotopic composition on concentration of oil-oxidizing actinobacteria Rhodococcus erythropolis VKM Ac-2017D cellular biomass is observed during cultivation in liquid nutrient media. The value for the effect was determined by experimental conditions, including consecutive use of hydrophilic and hydrophobic nutritious substrates--saccharose and hexadecane. It is shown, that when Rhodococcus erythropolis VKM Ac-2017D cells, cultivated in media with sucrose and 71 and 98 ppm deuterium content in water were inoculated into the similar in mineral and isotopic composition media with hexadecane, a considerable increase in cellular biomass production is observed if compared to a control sample in which water with 150 ppm deuterium concentration was used.

We analyzed the effects of weak combined magnetic fields, tuned to the cyclotron resonance condition for calcium ions, obtained in different phases of planarian regeneration. We showed that the result of regeneration in 72 hours after decapitation depends on the length of exposure, and the time between decapitation and initiation of a half-hour exposure. The experimental dependence can be explained by a multiplicity of enzymatic targets activated in different phases of the regeneration process.

The inhibitory action of binuclear dinitrosyl iron complexes with glutathione on the growth of implanted solid tumor in BDF1 mice bearing Lewis lung carcinoma cells was found. The effect was induced by intraperitoneal injection of the binuclear dinitrosyl iron complexes to mice at a dose of 200 μM/kg daily on days 1-5 and 7-11. At the binuclear dinitrosyl iron complexes: free glutathione ratios of 1:1; and 1:10 in solutions, the inhibitory effect of the DNICs reached the level of 70% and 85%, respectively. When B-DNICs were not further infused, intensive tumor growth, a more rapid rate of tumor growth than control, was observed. The selective accumulation of DNICs as well as iron nitrosyl complexes of heme-containing proteins in tumors were detected by EPR method. The latter were found also in the tumors in control animals. Tumor growth delay in course of B-DNIC administration to the mice is supposed to be due to the elaboration of anti-nitrosative defense in tumor tissue in response to the action of NO released from B-DNIC.

The work deals with spectra of capillary waves of water distillate and water solution of NADH and dynamics of NADH under electromagnetic irradiation in the range of millimeters at different space frequencies of surface wave k.

The dependence G(t) of electrical conductance G of Millipore water on time t was studied on its cooling down below 25 degrees C. The water was placed into a stainless, non-hermetic, cylindrical container with a working volume of 650 ml. The study was conducted by the ac capacitance method with immersion of the measuring electrodes in the studied water. It was found, that the change in the distribution of temperature gradient in the vertical direction of the investigated volume of water leads to the abnormal large change in the value of water conductance. It was also shown, that the contact with atmosphere is not a determining factor for the existence of the observed phenomenon.

Electron spin resonance (ESR) studies were carried out for 14N-labeled deuterated 3-methoxy-carbonyl-2,2,5,5-tetramethyl-pyrrolidine-1-oxyl (MC-PROXYL) and 3-carboxy-2,2,5,5-tetramethyl-1-pyrrolidin-1-oxyl (carboxy-PROXYL) in pure water and various concentrations of liposomal solutions by using 300 MHz ESR spectrometer. The ESR parameters such as the line width, hyperfine coupling constant, rotational correlation time, g-factor, partition parameter and permeability were reported for the samples. The line width broadening was observed for MC-PROXYL and carboxy-PROXYL in liposomal solution. The hyperfine coupling constant was observed for both nitroxyl spin probes. The permeable and impermeable nature of nitroxyl spin probes was demonstrated. The rotational correlation time increases with increasing concentration of liposome. The partition parameter increases with increasing concentration of liposome for MC-PROXYL, which indicates that the nitroxyl spin probes diffuse into lipid membrane. The permeability value decreases with increasing concentration of liposome, which reveals an increase in membrane permeability. The peaks corresponding to the lipid phase were observed for MC-PROXYL in liposomal solution, but not resolved for carboxy-PROXYL. These results confirm the permeable and impermeable nature of nitroxyl spin probes.

In this work, we investigated the effect of dilution on aggregation of nanoparticles of the polycarboxylic derivative of fullerene C60. It is shown that the diminution of the concentration of PCDF-1 in aqueous medium leads to a decreased amount of aggregates of fullerene and an increased amount of single molecules. This can potentially interfere with the biological activity of a compound on one molecule basis. Addition of organic and inorganic salts to the aqueous medium with fullerene derivative leads to intense disaggregation of PCDF-1. The data obtained suggest an explanation of non-stoichiometric nature of neutralization of reactive oxygen species by derivatives of fullerenes, as well as provide new insight into the physical meaning of the work on the impact of nanoparticles at ultra-low concentrations on biological objects.

Methods of construction of support vector machines require no further a priori infoimation and provide big data processing, what is especially important for various problems in computational biology. The question of the quality of learning algorithms is considered. The main algorithms of support vector machines for binary classification are reviewed and they were comparatively explored for their efficiencies. The critical analysis of the results of this study revealed the most effective support-vector-classifiers. The description of the recommended algorithms, sufficient for their practical implementation, is presented.