M. Matsui, Tatsuya Yokoyama, Toshitaka Kumagai, K. Nemoto, G. Terai, M. Machida, Takashi Shibata, S. Aburatani
{"title":"Genomic Analysis of Fungal Species No.11243 Mutant Strains Provides Insights into the Relationship between Mutations and High Productivity","authors":"M. Matsui, Tatsuya Yokoyama, Toshitaka Kumagai, K. Nemoto, G. Terai, M. Machida, Takashi Shibata, S. Aburatani","doi":"10.9734/bbj/2016/19400","DOIUrl":"https://doi.org/10.9734/bbj/2016/19400","url":null,"abstract":"","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"10 1","pages":"1-13"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71163604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. S. Rajan, A. Kamboj, B. R. Prusty, T. K. Palai, L. Ananthakrishna, M. Saini, P. Gupta
Aim: To produce bovine herpesvirus-1 (BoHV-1) vaccine strains in MDBK cells using an oscillating bioreactor, BelloCell. Place and Duration of Study: Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar-243122, India, between November 2013 and June 2015. Methodology: MDBK cells were cultured in BelloCell bioreactor seeded with MDBK cells. After 6 days incubation at 37°C under 5% CO 2 tension, the MDBK cells were infected with 0.001 multiplicity of infection (MOI) of either wild-type (wt)-BoHV-1 or glycoprotein E deleted (∆gE)-BoHV-1vaccine strains and incubated further for 96 h. To analyse the vaccine potential of the harvests, inactivated wt-BoHV-1 and ∆gE-BoHV-1 were inoculated in guinea pigs. Sera from immunized guinea pigs were collected at 90 days after primary immunization and analysed for virus neutralizing (VN) antibody response specific to wt-BoHV-1. Results: Using BelloCell bioreactor, the MDBK cell density reached to a maximum number of Original Research Article Rajan et al.; BBJ, 12(1): 1-7, 2016; Article no.BBJ.20401 2 1.5 x 10 to 2.0 x 10 cells after 6 days cultivation. After infection with wt-BoHV-1 and ∆gE-BoHV-1 strains, the total virus yield at 96 h post-infection was 0.5 x 10 and 0.5 x 10, respectively. There was no significant difference in VN antibody response in sera collected from immunized guinea pigs. Conclusion: This study indicated that BelloCell bioreactor can be used as a simple system for high population density cell culture and large scale production of vaccines.
{"title":"Production of Bovine Herpesvirus-1 Vaccine Strains in MDBK Cells Using BelloCell Bioreactor","authors":"L. S. Rajan, A. Kamboj, B. R. Prusty, T. K. Palai, L. Ananthakrishna, M. Saini, P. Gupta","doi":"10.9734/bbj/2016/20401","DOIUrl":"https://doi.org/10.9734/bbj/2016/20401","url":null,"abstract":"Aim: To produce bovine herpesvirus-1 (BoHV-1) vaccine strains in MDBK cells using an oscillating bioreactor, BelloCell. Place and Duration of Study: Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar-243122, India, between November 2013 and June 2015. Methodology: MDBK cells were cultured in BelloCell bioreactor seeded with MDBK cells. After 6 days incubation at 37°C under 5% CO 2 tension, the MDBK cells were infected with 0.001 multiplicity of infection (MOI) of either wild-type (wt)-BoHV-1 or glycoprotein E deleted (∆gE)-BoHV-1vaccine strains and incubated further for 96 h. To analyse the vaccine potential of the harvests, inactivated wt-BoHV-1 and ∆gE-BoHV-1 were inoculated in guinea pigs. Sera from immunized guinea pigs were collected at 90 days after primary immunization and analysed for virus neutralizing (VN) antibody response specific to wt-BoHV-1. Results: Using BelloCell bioreactor, the MDBK cell density reached to a maximum number of Original Research Article Rajan et al.; BBJ, 12(1): 1-7, 2016; Article no.BBJ.20401 2 1.5 x 10 to 2.0 x 10 cells after 6 days cultivation. After infection with wt-BoHV-1 and ∆gE-BoHV-1 strains, the total virus yield at 96 h post-infection was 0.5 x 10 and 0.5 x 10, respectively. There was no significant difference in VN antibody response in sera collected from immunized guinea pigs. Conclusion: This study indicated that BelloCell bioreactor can be used as a simple system for high population density cell culture and large scale production of vaccines.","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"12 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71163649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Purification and Partial Characterization of β-Galactosidase from Cajanus cajans (Pigeon Pea)","authors":"A. Dayo, O. Abiola, O. Azuka","doi":"10.9734/bbj/2016/21345","DOIUrl":"https://doi.org/10.9734/bbj/2016/21345","url":null,"abstract":"","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"14 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71163736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Atere, Franklin Kayode Ayenogun, B. D. Akinbo, A. Okafor, K. I. Egbuchulem
{"title":"Assessment of Methaemoglobin and Carboxyhaemoglobin Levels among Pregnant Women Infected with Hepatitis B Virus","authors":"A. Atere, Franklin Kayode Ayenogun, B. D. Akinbo, A. Okafor, K. I. Egbuchulem","doi":"10.9734/bbj/2016/21880","DOIUrl":"https://doi.org/10.9734/bbj/2016/21880","url":null,"abstract":"","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"10 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71163905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fokunang Charles, M. Grace, M. Evelyn, T. Estella, R. Hanna
Introduction: Taro leaf blight disease cause by Phytophtora colocasiae has become an economic disease in Cocoyam growing regions of Cameroon. Aims: To screen for resistance 10 improved and 4 local cultivars of taro against taro leaf blight disease. Study Design: A randomized complete block design study. Place of Study: Studies were conducted at the International Institute of Tropical Agriculture (IITA) Yaounde Nkolbisson from July 2013 to January 2014. Methodology: Taro cultivars from tissue culture were planted in the screen house conditions and Original Research Article Charles et al.; BBJ, 15(1): 1-15, 2016; Article no.BBJ.14317 2 tested for virulence and pathogenicity with 4 isolates of Phythophthora colocasiae at spore density of 3×10 4 spores /ml of distilled water. Plants were planted in the field to assess disease incidence and severity. Results: The results obtained on the different taro cultivars, revealed that all the 4 isolates showed variable pathogenicity. They caused lesions on inoculated leaves. There was variability in pathogenicity based on the small lesion lengths produced on cultivars, these included BL/SM132 and Red petiole. Isolate 3 showed a stronger sensitivity to leaf collapse and defoliation irrespective of the cultivar tested. There was a significant difference (p = 0.05) in tissue collapse and leaf defoliation on exposure to the different fungal isolates. The result of field infection rates of P. colocasiae at 126 DAP-154 DAP on 10 improved and 4 local cultivars indicated that there was significant variability (p = 0.05) in incidence and disease severity, with high incidence and severity occurring at 154 DAP in all cultivars. Improved cultivar BL/SM132 showed no classic symptoms of P. colocasiae and therefore it was resistant to Phytophthora colocasiae. Conclusion: The results obtained on virulence and pathogenicity of Phythophthora colocasiae on the different taro cultivars revealed that all the 4 isolates showed variable pathogenicity. They caused lesions, on inoculated leaves. Isolate 3 showed a stronger sensitivity to leaf collapse and defoliation irrespective of the cultivar tested. The result of field infection rates of P. colocasiae at 126 DAP-154 DAP on 10 improved and 4 local cultivars indicated that there was a significant variability (p = 0.05) in disease incidence and severity, with high incidence and severity occurring at 154 DAP in all cultivars. Improved cultivar BL/SM132 showed no classic symptoms of P. colocasiae and therefore it was resistant to Phytophthora colocasiae as compared to all the other cultivars which showed high severity rates of infection of the disease and thus were susceptible to the disease.
{"title":"Screen house and field resistance of taro cultivars to taro leaf blight disease (Phytophtora colocasiae)","authors":"Fokunang Charles, M. Grace, M. Evelyn, T. Estella, R. Hanna","doi":"10.9734/BBJ/2016/14317","DOIUrl":"https://doi.org/10.9734/BBJ/2016/14317","url":null,"abstract":"Introduction: Taro leaf blight disease cause by Phytophtora colocasiae has become an economic disease in Cocoyam growing regions of Cameroon. Aims: To screen for resistance 10 improved and 4 local cultivars of taro against taro leaf blight disease. Study Design: A randomized complete block design study. Place of Study: Studies were conducted at the International Institute of Tropical Agriculture (IITA) Yaounde Nkolbisson from July 2013 to January 2014. Methodology: Taro cultivars from tissue culture were planted in the screen house conditions and Original Research Article Charles et al.; BBJ, 15(1): 1-15, 2016; Article no.BBJ.14317 2 tested for virulence and pathogenicity with 4 isolates of Phythophthora colocasiae at spore density of 3×10 4 spores /ml of distilled water. Plants were planted in the field to assess disease incidence and severity. Results: The results obtained on the different taro cultivars, revealed that all the 4 isolates showed variable pathogenicity. They caused lesions on inoculated leaves. There was variability in pathogenicity based on the small lesion lengths produced on cultivars, these included BL/SM132 and Red petiole. Isolate 3 showed a stronger sensitivity to leaf collapse and defoliation irrespective of the cultivar tested. There was a significant difference (p = 0.05) in tissue collapse and leaf defoliation on exposure to the different fungal isolates. The result of field infection rates of P. colocasiae at 126 DAP-154 DAP on 10 improved and 4 local cultivars indicated that there was significant variability (p = 0.05) in incidence and disease severity, with high incidence and severity occurring at 154 DAP in all cultivars. Improved cultivar BL/SM132 showed no classic symptoms of P. colocasiae and therefore it was resistant to Phytophthora colocasiae. Conclusion: The results obtained on virulence and pathogenicity of Phythophthora colocasiae on the different taro cultivars revealed that all the 4 isolates showed variable pathogenicity. They caused lesions, on inoculated leaves. Isolate 3 showed a stronger sensitivity to leaf collapse and defoliation irrespective of the cultivar tested. The result of field infection rates of P. colocasiae at 126 DAP-154 DAP on 10 improved and 4 local cultivars indicated that there was a significant variability (p = 0.05) in disease incidence and severity, with high incidence and severity occurring at 154 DAP in all cultivars. Improved cultivar BL/SM132 showed no classic symptoms of P. colocasiae and therefore it was resistant to Phytophthora colocasiae as compared to all the other cultivars which showed high severity rates of infection of the disease and thus were susceptible to the disease.","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"52 1","pages":"1-15"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71163997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Relationship of Biomass and Xanthan Gum Production by Xanthomonas campestris: Optimization of Parameters","authors":"S. Chavan, M. Baig","doi":"10.9734/bbj/2016/22431","DOIUrl":"https://doi.org/10.9734/bbj/2016/22431","url":null,"abstract":"","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"11 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71164116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of Processing on in–vitro Protein Digestibility and Anti–nutritional Properties of Three Underutilized Legumes Grown in Nigeria","authors":"M. Aremu, H. Ibrahim, B. Ekanem","doi":"10.9734/BBJ/2016/22581","DOIUrl":"https://doi.org/10.9734/BBJ/2016/22581","url":null,"abstract":"","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"14 1","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71164222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of Cytokinins on In vitro Propagation of Boucerosia umbellata (Haw.) Wight & Arn. (Syn.: Caralluma umbellata Haw.) from Nodal Explants of Field Grown Plants","authors":"B. Susheela, S. Rani, T. Pullaiah","doi":"10.9734/bbj/2016/23676","DOIUrl":"https://doi.org/10.9734/bbj/2016/23676","url":null,"abstract":"","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"12 1","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71164464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of Stocking Density on the Performance, Carcass Yield and Meat Composition of Broiler Chickens","authors":"G. Adeyemo, O. Fashola, T. Ademulegun","doi":"10.9734/BBJ/2016/24372","DOIUrl":"https://doi.org/10.9734/BBJ/2016/24372","url":null,"abstract":"","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"14 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71164821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Dietary Effect of Different Drying Methods and Graded Inclusion Levels of Ginger (Zingiber officinale) on the Performance and Gut Morphology of Broilers","authors":"G. Adeyemo, E. Ogunshote, O. Longe","doi":"10.9734/BBJ/2016/24716","DOIUrl":"https://doi.org/10.9734/BBJ/2016/24716","url":null,"abstract":"","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"15 1","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71164873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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