M. Al-Jailawi, Amer Al-Shekdhaher, Rahem. E. AL-Zaiadi
{"title":"Genetic Improvement of Saccharomyces boulardii R7 and Generate Suitable Strains for Synthesis and Expression of Recombinant Products","authors":"M. Al-Jailawi, Amer Al-Shekdhaher, Rahem. E. AL-Zaiadi","doi":"10.9734/bbj/2016/22927","DOIUrl":"https://doi.org/10.9734/bbj/2016/22927","url":null,"abstract":"","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"11 1","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71164336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. M. M. A. Naggar, R. Shabana, M. Aleem, M. El-Aleem, Zainab A. El Rashidy, Z. El-Rashidy
{"title":"Heterobeltiosis in Wheat (Triticum aestivum L.) F 1 Diallel Crosses under Contrasting Soil-N Conditions","authors":"A. M. M. A. Naggar, R. Shabana, M. Aleem, M. El-Aleem, Zainab A. El Rashidy, Z. El-Rashidy","doi":"10.9734/BBJ/2016/21916","DOIUrl":"https://doi.org/10.9734/BBJ/2016/21916","url":null,"abstract":"","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"69 1","pages":"1-12"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71164426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Production and Characterization of Polyhydroxyalkanoate (PHA) Using Mango Seed Kernel as an Alternative to Glucose","authors":"K. Nasir-Naeem, K. Shittu, A. Kabiru","doi":"10.9734/BBJ/2016/25112","DOIUrl":"https://doi.org/10.9734/BBJ/2016/25112","url":null,"abstract":"","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"13 1","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71164606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim: This study assesses the phytochemical profiles, toxicity and anti-nutrient properties of two indigenous vegetables, Cleome gynandra and Solanum nigrum. Methodology: Aerial parts of S. nigrum and C. gynandra collected from wild populations were extracted in water and methanol solvents using the cold maceration procedure. Chemical tests to identify different phytochemicals and antinutrients were carried out using specified reagents. Toxicity of the plant extracts was tested using the Brine Shrimp (Artemia salina) lethality bioassay procedure. Results: Phytochemical tests indicated presence of alkaloids, steroids, flavonoids, cardiac glycosides, saponins, phenols and tannins in both S. nigrum and C. gynandra. Anthraquinones were only found in C. gynandra, whereas terpenoids and coumarin glycosides were found only in Original Research Article Sango et al.; BBJ, 13(3): 1-11, 2016; Article no.BBJ.25164 2 S. nigrum. The anti-nutrient levels in the vegetables were high; tannins (3.580 and 1.820 mg/ 100 g), oxalates (21.560 and 24.97±0.156 g/100 g), saponins (71 and 42%), alkaloids (15.160 and 3.44%) and phytates (0.099±0.046 and 0.250±0.013%) in S. nigrum and C. gynandra respectively. The Brine Shrimp LC50 values obtained were 0.67 and 1.84 mg/ml for methanol and water extracts of S. nigrum and 1.09 and 4.9 mg/ml for C. gynandra, respectively. Conclusion: The vegetables, C. gynandra and S. nigrum are rich sources of diverse phytochemicals. However, some phytochemicals are present in high levels and might act as anti nutritional factors interfering with absorption of food. C. gynandra is safe for consumption but S. nigrum contains some toxic phytochemicals and should not be taken regularly.
{"title":"Phytochemical, Anti-nutrients and Toxicity Evaluation of Cleome gynandra and Solanum nigrum: Common Indigenous Vegetables in Zimbabwe","authors":"Caroline Sango, L. Marufu, C. Zimudzi","doi":"10.9734/BBJ/2016/25164","DOIUrl":"https://doi.org/10.9734/BBJ/2016/25164","url":null,"abstract":"Aim: This study assesses the phytochemical profiles, toxicity and anti-nutrient properties of two indigenous vegetables, Cleome gynandra and Solanum nigrum. Methodology: Aerial parts of S. nigrum and C. gynandra collected from wild populations were extracted in water and methanol solvents using the cold maceration procedure. Chemical tests to identify different phytochemicals and antinutrients were carried out using specified reagents. Toxicity of the plant extracts was tested using the Brine Shrimp (Artemia salina) lethality bioassay procedure. Results: Phytochemical tests indicated presence of alkaloids, steroids, flavonoids, cardiac glycosides, saponins, phenols and tannins in both S. nigrum and C. gynandra. Anthraquinones were only found in C. gynandra, whereas terpenoids and coumarin glycosides were found only in Original Research Article Sango et al.; BBJ, 13(3): 1-11, 2016; Article no.BBJ.25164 2 S. nigrum. The anti-nutrient levels in the vegetables were high; tannins (3.580 and 1.820 mg/ 100 g), oxalates (21.560 and 24.97±0.156 g/100 g), saponins (71 and 42%), alkaloids (15.160 and 3.44%) and phytates (0.099±0.046 and 0.250±0.013%) in S. nigrum and C. gynandra respectively. The Brine Shrimp LC50 values obtained were 0.67 and 1.84 mg/ml for methanol and water extracts of S. nigrum and 1.09 and 4.9 mg/ml for C. gynandra, respectively. Conclusion: The vegetables, C. gynandra and S. nigrum are rich sources of diverse phytochemicals. However, some phytochemicals are present in high levels and might act as anti nutritional factors interfering with absorption of food. C. gynandra is safe for consumption but S. nigrum contains some toxic phytochemicals and should not be taken regularly.","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"13 1","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71164619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Nakashima, K. Homma, Michiko Yamazaki, M. Ishizaki, K. Mawatari
Aims: To reveal which amino acid residues determine whether a protein is acidic or basic between orthologous pairs, acidic proteins from halophilic species and corresponding basic proteins from non-halophilic species were compared. Similarly acidic versus acidic protein pairs, and basic versus basic protein pairs were also analyzed. Place and Duration of Study: Department of Clinical Laboratory Science, Graduate Course of Medical Science and Technology, School of Health Sciences, Kanazawa University, Japan. Methodology: Halobacterium sp. NRC-1 was used as halophilic species and Gram-positive bacterium Bacillus subtilis, and radiation resistant bacterium Deinococcus radiodurans were used as non-halophilic species. The three species were selected because their proteins were closely Original Research Article Nakashima et al.; BBJ, 14(2): 1-12, 2016; Article no.BBJ.25207 2 related each other. The amino acid compositions were compared and the amino acid substitutions were counted for the orthologous protein pairs between Halobacterium and B. subtilis. Similar comparison was done for the proteins between Halobacterium and D. radiodurans. Results: The Asp and Glu residues are determinant whether a protein of Halobacterium sp. NRC-1 is acidic or basic. Amino acid substitutions to increase the Asp residues in the acidic proteins of Halobacterium from the corresponding proteins of non-halophilic species were almost identical whether the corresponding proteins were acidic or basic. This result suggested that the change of protein charges from basic proteins to acidic ones was same as from acidic proteins to acidic ones. The proteins of Halobacterium showed a tendency to have residues with smaller side chain than the proteins of B. subtilis / D. radiodurans.
{"title":"Database Analysis of Acidic Proteins from Halophilic Species and Their Corresponding Basic Proteins from Non-halophilic Species","authors":"H. Nakashima, K. Homma, Michiko Yamazaki, M. Ishizaki, K. Mawatari","doi":"10.9734/bbj/2016/25207","DOIUrl":"https://doi.org/10.9734/bbj/2016/25207","url":null,"abstract":"Aims: To reveal which amino acid residues determine whether a protein is acidic or basic between orthologous pairs, acidic proteins from halophilic species and corresponding basic proteins from non-halophilic species were compared. Similarly acidic versus acidic protein pairs, and basic versus basic protein pairs were also analyzed. Place and Duration of Study: Department of Clinical Laboratory Science, Graduate Course of Medical Science and Technology, School of Health Sciences, Kanazawa University, Japan. Methodology: Halobacterium sp. NRC-1 was used as halophilic species and Gram-positive bacterium Bacillus subtilis, and radiation resistant bacterium Deinococcus radiodurans were used as non-halophilic species. The three species were selected because their proteins were closely Original Research Article Nakashima et al.; BBJ, 14(2): 1-12, 2016; Article no.BBJ.25207 2 related each other. The amino acid compositions were compared and the amino acid substitutions were counted for the orthologous protein pairs between Halobacterium and B. subtilis. Similar comparison was done for the proteins between Halobacterium and D. radiodurans. Results: The Asp and Glu residues are determinant whether a protein of Halobacterium sp. NRC-1 is acidic or basic. Amino acid substitutions to increase the Asp residues in the acidic proteins of Halobacterium from the corresponding proteins of non-halophilic species were almost identical whether the corresponding proteins were acidic or basic. This result suggested that the change of protein charges from basic proteins to acidic ones was same as from acidic proteins to acidic ones. The proteins of Halobacterium showed a tendency to have residues with smaller side chain than the proteins of B. subtilis / D. radiodurans.","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"14 1","pages":"1-12"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71164681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Agbebi, C. Echefu, I. Adeosun, A. Ajibade, E. A. Adegbite, A. Adebambo, M. B. Ilori, So Durosaro, A. B. Ajibike
Aim: The aims are to study the genetic diversity and population structure of Tilapia fish species in Nigeria using mtDNA D-loop region and time divergence of these various groups of fish to give a baseline information for fish management and conservation programs. Methodology: Blood samples from four species of tilapia were collected with the use of FTA cards for DNA extraction and PCR amplification. A 941bp long fragment, including the D-loop, was subsequently used for analysis. Molecular diversity indices and Tajima’s selective test were determined. A phylogenetic tree was constructed for all the four fish species using UPGMA. The divergence time among the four fish species using MEGA5 software.
目的:研究尼日利亚罗非鱼种群的遗传多样性和种群结构，利用mtDNA d -环区和这些不同种群的时间散度，为鱼类管理和保护计划提供基线信息。方法:采用FTA卡采集4种罗非鱼的血样，进行DNA提取和PCR扩增。一个941bp长的片段，包括D-loop，随后被用于分析。测定分子多样性指数和田岛选择试验。利用UPGMA构建了4种鱼类的系统发育树。利用MEGA5软件计算四种鱼类的分化时间。
{"title":"Mitochondrial Diversity and Time Divergence of Commonly Cultured Cichlids in Nigeria","authors":"O. Agbebi, C. Echefu, I. Adeosun, A. Ajibade, E. A. Adegbite, A. Adebambo, M. B. Ilori, So Durosaro, A. B. Ajibike","doi":"10.9734/BBJ/2016/25470","DOIUrl":"https://doi.org/10.9734/BBJ/2016/25470","url":null,"abstract":"Aim: The aims are to study the genetic diversity and population structure of Tilapia fish species in Nigeria using mtDNA D-loop region and time divergence of these various groups of fish to give a baseline information for fish management and conservation programs. Methodology: Blood samples from four species of tilapia were collected with the use of FTA cards for DNA extraction and PCR amplification. A 941bp long fragment, including the D-loop, was subsequently used for analysis. Molecular diversity indices and Tajima’s selective test were determined. A phylogenetic tree was constructed for all the four fish species using UPGMA. The divergence time among the four fish species using MEGA5 software.","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"97 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71164756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Ramesh, B. S. Chouhan, G. Gupta, R. Ramteke, S. Chand, S. M. Husain
Aim: Coat protein (CP) genes encoded by Legume yellow mosaic viruses (LYMVs) were analysed to study molecular diversity and to devise effective PCR based assay to distinguish major Begomovirus species ( Mungbean yellow mosaic India virus and Mungbean yellow mosaic virus ) infecting soybean Design of the Study: All the known coat protein gene sequences encoded by begomoviruses causing yellow mosaic disease (YMD) in legumes were obtained from GenBank. YMD infected soybean leaf samples were collected from different parts of India during Kharif 2012 and species of virus infections identified using CP gene based primers in a PCR assay.
{"title":"Molecular Diversity Analysis of Coat Protein Gene Encoded by Legume Begomoviruses and PCR Assay to Detect Yellow Mosaic Viruses Infecting Soybean in India","authors":"S. Ramesh, B. S. Chouhan, G. Gupta, R. Ramteke, S. Chand, S. M. Husain","doi":"10.9734/BBJ/2016/24362","DOIUrl":"https://doi.org/10.9734/BBJ/2016/24362","url":null,"abstract":"Aim: Coat protein (CP) genes encoded by Legume yellow mosaic viruses (LYMVs) were analysed to study molecular diversity and to devise effective PCR based assay to distinguish major Begomovirus species ( Mungbean yellow mosaic India virus and Mungbean yellow mosaic virus ) infecting soybean Design of the Study: All the known coat protein gene sequences encoded by begomoviruses causing yellow mosaic disease (YMD) in legumes were obtained from GenBank. YMD infected soybean leaf samples were collected from different parts of India during Kharif 2012 and species of virus infections identified using CP gene based primers in a PCR assay.","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"12 1","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71164809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim: The present study was conducted to evaluate the antifungal activity of camel faeces on some pathogenic fungi. Study Design: This is a descriptive evaluation study. Methodology: Camel faeces was extracted following Harborne method using organic solvents. Organic extracts besides, aqueous extract and ash were screened against clinical isolates using agar-well diffusion and incorporated methods. Parallel experiments were conducted with ketoconazole and nystatin, as positive control whereas; the vehicle solvents were used as negative control. Phytochemical analysis of Camel feaces was carried out following Harborne method. Short Research Article Suleiman et al.; BBJ, 13(2): 1-5, 2016; Article no.BBJ.24744 2 Results: Water and ethanol extracts exerted significant effect on dermatophytes followed by chloroform and hexane extracts compared to the ash which revealed no activity. Aspergillus and Pencillium species were found insensitive to all test extracts where as Candida albicans was found sensitive only to the hexane extract. Sterols and triterpenes were revealed on phytochemical analysis. Discussion: The antifungal activity of camel faeces might be due to the sterols and triterpenes. Conclusion: The study confirms efficacy of camel faeces as natural antifungal agent, and suggests the possibility of employing it for treatment of skin infections, caused by the test pathogens. The present study reveals first report on the use of camel faeces against some pathogenic fungi. Recommendation: Identification and characterization of novel molecules are highly recommended.
{"title":"Antifungal Activity of Camel Faeces with Special Reference to Dermatophytes","authors":"E. Suleiman, M. Kabashi, S. Elbashir, A. Elhassan","doi":"10.9734/BBJ/2016/24744","DOIUrl":"https://doi.org/10.9734/BBJ/2016/24744","url":null,"abstract":"Aim: The present study was conducted to evaluate the antifungal activity of camel faeces on some pathogenic fungi. Study Design: This is a descriptive evaluation study. Methodology: Camel faeces was extracted following Harborne method using organic solvents. Organic extracts besides, aqueous extract and ash were screened against clinical isolates using agar-well diffusion and incorporated methods. Parallel experiments were conducted with ketoconazole and nystatin, as positive control whereas; the vehicle solvents were used as negative control. Phytochemical analysis of Camel feaces was carried out following Harborne method. Short Research Article Suleiman et al.; BBJ, 13(2): 1-5, 2016; Article no.BBJ.24744 2 Results: Water and ethanol extracts exerted significant effect on dermatophytes followed by chloroform and hexane extracts compared to the ash which revealed no activity. Aspergillus and Pencillium species were found insensitive to all test extracts where as Candida albicans was found sensitive only to the hexane extract. Sterols and triterpenes were revealed on phytochemical analysis. Discussion: The antifungal activity of camel faeces might be due to the sterols and triterpenes. Conclusion: The study confirms efficacy of camel faeces as natural antifungal agent, and suggests the possibility of employing it for treatment of skin infections, caused by the test pathogens. The present study reveals first report on the use of camel faeces against some pathogenic fungi. Recommendation: Identification and characterization of novel molecules are highly recommended.","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"251 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71164896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Sharifi‐Rad, S. Hoseini-Alfatemi, A. Miri, M. Sharifi-Rad, M. Sharifi-Rad, M. Hoseini, M. Sharifi-Rad
Zabol Medicinal Plants Research Center, Zabol University of Medical Sciences, Zabol, Iran. Department of Pharmacognosy, Faculty of Pharmacy, Zabol University of Medical Sciences, Zabol, Iran. Pediatric Infections Research Center, Mofid Children Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Department of Range and Watershed Management, Faculty of Natural Resources, University of Zabol, Iran. Department of Chemistry, Faculty of Science, University of Zabol, Zabol 98615-538, Iran. Department of Food Science and Technology, Faculty of Agriculture, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran. Zabol University of Medical Sciences, Zabol, Iran.
{"title":"Exploration of Phytochemical and Antibacterial Potentiality of Anagallis arvensis L. Extract against Methicillin-Resistant Staphylococcus aureus (MRSA)","authors":"J. Sharifi‐Rad, S. Hoseini-Alfatemi, A. Miri, M. Sharifi-Rad, M. Sharifi-Rad, M. Hoseini, M. Sharifi-Rad","doi":"10.9734/BBJ/2016/20505","DOIUrl":"https://doi.org/10.9734/BBJ/2016/20505","url":null,"abstract":"Zabol Medicinal Plants Research Center, Zabol University of Medical Sciences, Zabol, Iran. Department of Pharmacognosy, Faculty of Pharmacy, Zabol University of Medical Sciences, Zabol, Iran. Pediatric Infections Research Center, Mofid Children Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Department of Range and Watershed Management, Faculty of Natural Resources, University of Zabol, Iran. Department of Chemistry, Faculty of Science, University of Zabol, Zabol 98615-538, Iran. Department of Food Science and Technology, Faculty of Agriculture, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran. Zabol University of Medical Sciences, Zabol, Iran.","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"10 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71163678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Biotechnological Application of Cassava-Degrading Fungal (CDF) Amylase in Broiler Feed Formulation","authors":"O. Avwioroko, A. Anigboro, N. J. Tonukari","doi":"10.9734/bbj/2016/20614","DOIUrl":"https://doi.org/10.9734/bbj/2016/20614","url":null,"abstract":"","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"10 1","pages":"1-12"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71163723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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