{"title":"Spectrophotometric Determination of Sun Protection Factor and Antioxidant Potential of an Herbal Mixture","authors":"Madhulika Singh, Varsha Sharma","doi":"10.9734/bbj/2016/21434","DOIUrl":"https://doi.org/10.9734/bbj/2016/21434","url":null,"abstract":"","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"10 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71163793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fatma Meddeb-Mouelhi, Jessica Kelly Moisan, M. Beauregard
{"title":"Characterization of Bacteria Community Isolated from Wood Decay","authors":"Fatma Meddeb-Mouelhi, Jessica Kelly Moisan, M. Beauregard","doi":"10.9734/bbj/2016/21512","DOIUrl":"https://doi.org/10.9734/bbj/2016/21512","url":null,"abstract":"","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"7 1","pages":"1-14"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71163804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aims: Isolation and identification of Streptococcus pneumoniae from children with acute otitis media. Molecular detection of some virulence genes of S. pneumoniae such as pneumolysin (ply), autolysin A (LytA), Neuraminidase (NanA), Luminescence luxS, capsular polysaccharide synthesis (cpsA), pneumococcal surface antigen A (psaA), αEnolase (Eno ) by using PCR primer. Place and Duration of Study: Hilla Teaching Hospital (ENT unit) and privacy during a period from November (2014) to March (2015), Hilla, Iraq. Methodology: A total 0f 120 Ear swabs obtained from children suffering from acute otitis media ranged from (1 months 12 years). Only eight isolates of S. pneumoniae were isolates. Seven virulence genes were investigated in eight clinical isolates of S. pneumoniae by using PCR techniques. Results: Pneumolysin and autolysin were seen to be present in only 4 isolates which were isolated from otitis media samples, whereas Luminescence and α Enolase gene were present in five Original Research Article Abdul-Lateef et al.; BBJ, 10(3): 1-11, 2016; Article no.BBJ.22033 2 isolates, Besides, Neuraminidase and pneumococcal capsule synthesis A were detected in all isolates. It was found that Pneumococcal Surface antigen A is present in 2 isolates.
{"title":"Molecular Characterization of Some Virulence Factors of Streptococcus pneumoniae Isolated from Children with Acute Otitis Media in Hilla, Iraq","authors":"L. Abdul-Lateef, S. Alturaihy, Shaima Al-Taai","doi":"10.9734/bbj/2016/22033","DOIUrl":"https://doi.org/10.9734/bbj/2016/22033","url":null,"abstract":"Aims: Isolation and identification of Streptococcus pneumoniae from children with acute otitis media. Molecular detection of some virulence genes of S. pneumoniae such as pneumolysin (ply), autolysin A (LytA), Neuraminidase (NanA), Luminescence luxS, capsular polysaccharide synthesis (cpsA), pneumococcal surface antigen A (psaA), αEnolase (Eno ) by using PCR primer. Place and Duration of Study: Hilla Teaching Hospital (ENT unit) and privacy during a period from November (2014) to March (2015), Hilla, Iraq. Methodology: A total 0f 120 Ear swabs obtained from children suffering from acute otitis media ranged from (1 months 12 years). Only eight isolates of S. pneumoniae were isolates. Seven virulence genes were investigated in eight clinical isolates of S. pneumoniae by using PCR techniques. Results: Pneumolysin and autolysin were seen to be present in only 4 isolates which were isolated from otitis media samples, whereas Luminescence and α Enolase gene were present in five Original Research Article Abdul-Lateef et al.; BBJ, 10(3): 1-11, 2016; Article no.BBJ.22033 2 isolates, Besides, Neuraminidase and pneumococcal capsule synthesis A were detected in all isolates. It was found that Pneumococcal Surface antigen A is present in 2 isolates.","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"10 1","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71164010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Oliván, V. Fernández-Suárez, Fernando Díaz-Martínez, V. Sierra, A. Coto-Montes, B. Luxán-Delgado, Raquel Peña, A. Bassols, E. Fàbrega, A. Dalmau, A. Velarde
Aims: The objective of this work was to apply the proteomic approach for identification of animalbased stress biomarkers during pig muscle maturation. Study Design: Pigs were subjected to management treatments that may promote stress, like Original Research Article Oliván et al.; BBJ, 11(1): 1-13, 2016; Article no.BBJ.22402 2 mixing unfamiliar animals on farm and/or during transport and lairage before slaughter. Three preslaughter treatments were applied: UF-UTL (Unmixed on Farm Unmixed during Transport and at Lairage), MF-UTL (Mixed in Farm Unmixed during Transport and at Lairage) and MF-MTL (Mixed in Farm Mixed during Transport and at Lairage). Methodology: Fifteen entire male pigs were used in this experiment. At slaughter, blood samples were taken for biochemical analysis. Meat quality traits (pH, electrical conductivity, drip loss, meat colour) were measured and muscle samples from the Longissimus thoracis were taken within the first 24 h post-mortem and used for analysis of total antioxidant activity (TAA) and sarcoplasmic protein profile study by SDS-PAGE. Results: Mixing treatments (MF-UTL and MF-MTL) provoked faster muscle pH post-mortem decline (P= .009), lower total antioxidant activity (P< .001) and changes in some key proteins, like the heat shock 70kDa protein-1B (P= .040), glyceraldehyde-3-phosphate dehydrogenase “GAPDH” (P= .047), lactate dehydrogenase-A “LDH-A” (P< .001) and adenylate kinase isoenzyme-1 “AK-1” (P< .001). Furthermore, serum molecules related to fat metabolism (LDL-cholesterol and triglycerides) and changes of muscle proteins involved in the adenine nucleotide metabolism (AK-1) and glycolysis regulation (piruvate kinase “PK” and GAPDH) allowed accurate prediction of muscle pH and discriminated correctly 87% of meat samples into “no-mixing” or “mixing” treatments. Conclusions: Early monitorization (within 24 h after slaughter) of selected biomarkers could be used in the meat industry as a tool for detection of situations of pre-slaughter stress related to animal mixing practices. However more research is needed in order to validate these results on a broader animal population.
{"title":"Identification of Biomarkers of Stress in Meat of Pigs Managed under Different Mixing Treatments","authors":"M. Oliván, V. Fernández-Suárez, Fernando Díaz-Martínez, V. Sierra, A. Coto-Montes, B. Luxán-Delgado, Raquel Peña, A. Bassols, E. Fàbrega, A. Dalmau, A. Velarde","doi":"10.9734/bbj/2016/22402","DOIUrl":"https://doi.org/10.9734/bbj/2016/22402","url":null,"abstract":"Aims: The objective of this work was to apply the proteomic approach for identification of animalbased stress biomarkers during pig muscle maturation. Study Design: Pigs were subjected to management treatments that may promote stress, like Original Research Article Oliván et al.; BBJ, 11(1): 1-13, 2016; Article no.BBJ.22402 2 mixing unfamiliar animals on farm and/or during transport and lairage before slaughter. Three preslaughter treatments were applied: UF-UTL (Unmixed on Farm Unmixed during Transport and at Lairage), MF-UTL (Mixed in Farm Unmixed during Transport and at Lairage) and MF-MTL (Mixed in Farm Mixed during Transport and at Lairage). Methodology: Fifteen entire male pigs were used in this experiment. At slaughter, blood samples were taken for biochemical analysis. Meat quality traits (pH, electrical conductivity, drip loss, meat colour) were measured and muscle samples from the Longissimus thoracis were taken within the first 24 h post-mortem and used for analysis of total antioxidant activity (TAA) and sarcoplasmic protein profile study by SDS-PAGE. Results: Mixing treatments (MF-UTL and MF-MTL) provoked faster muscle pH post-mortem decline (P= .009), lower total antioxidant activity (P< .001) and changes in some key proteins, like the heat shock 70kDa protein-1B (P= .040), glyceraldehyde-3-phosphate dehydrogenase “GAPDH” (P= .047), lactate dehydrogenase-A “LDH-A” (P< .001) and adenylate kinase isoenzyme-1 “AK-1” (P< .001). Furthermore, serum molecules related to fat metabolism (LDL-cholesterol and triglycerides) and changes of muscle proteins involved in the adenine nucleotide metabolism (AK-1) and glycolysis regulation (piruvate kinase “PK” and GAPDH) allowed accurate prediction of muscle pH and discriminated correctly 87% of meat samples into “no-mixing” or “mixing” treatments. Conclusions: Early monitorization (within 24 h after slaughter) of selected biomarkers could be used in the meat industry as a tool for detection of situations of pre-slaughter stress related to animal mixing practices. However more research is needed in order to validate these results on a broader animal population.","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"11 1","pages":"1-13"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71164102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Al-Jailawi, Amer Al-Shekdhaher, Rahem. E. AL-Zaiadi
{"title":"Genetic Improvement of Saccharomyces boulardii R7 and Generate Suitable Strains for Synthesis and Expression of Recombinant Products","authors":"M. Al-Jailawi, Amer Al-Shekdhaher, Rahem. E. AL-Zaiadi","doi":"10.9734/bbj/2016/22927","DOIUrl":"https://doi.org/10.9734/bbj/2016/22927","url":null,"abstract":"","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"11 1","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71164336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. M. M. A. Naggar, R. Shabana, M. Aleem, M. El-Aleem, Zainab A. El Rashidy, Z. El-Rashidy
{"title":"Heterobeltiosis in Wheat (Triticum aestivum L.) F 1 Diallel Crosses under Contrasting Soil-N Conditions","authors":"A. M. M. A. Naggar, R. Shabana, M. Aleem, M. El-Aleem, Zainab A. El Rashidy, Z. El-Rashidy","doi":"10.9734/BBJ/2016/21916","DOIUrl":"https://doi.org/10.9734/BBJ/2016/21916","url":null,"abstract":"","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"69 1","pages":"1-12"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71164426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Production and Characterization of Polyhydroxyalkanoate (PHA) Using Mango Seed Kernel as an Alternative to Glucose","authors":"K. Nasir-Naeem, K. Shittu, A. Kabiru","doi":"10.9734/BBJ/2016/25112","DOIUrl":"https://doi.org/10.9734/BBJ/2016/25112","url":null,"abstract":"","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"13 1","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71164606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim: This study assesses the phytochemical profiles, toxicity and anti-nutrient properties of two indigenous vegetables, Cleome gynandra and Solanum nigrum. Methodology: Aerial parts of S. nigrum and C. gynandra collected from wild populations were extracted in water and methanol solvents using the cold maceration procedure. Chemical tests to identify different phytochemicals and antinutrients were carried out using specified reagents. Toxicity of the plant extracts was tested using the Brine Shrimp (Artemia salina) lethality bioassay procedure. Results: Phytochemical tests indicated presence of alkaloids, steroids, flavonoids, cardiac glycosides, saponins, phenols and tannins in both S. nigrum and C. gynandra. Anthraquinones were only found in C. gynandra, whereas terpenoids and coumarin glycosides were found only in Original Research Article Sango et al.; BBJ, 13(3): 1-11, 2016; Article no.BBJ.25164 2 S. nigrum. The anti-nutrient levels in the vegetables were high; tannins (3.580 and 1.820 mg/ 100 g), oxalates (21.560 and 24.97±0.156 g/100 g), saponins (71 and 42%), alkaloids (15.160 and 3.44%) and phytates (0.099±0.046 and 0.250±0.013%) in S. nigrum and C. gynandra respectively. The Brine Shrimp LC50 values obtained were 0.67 and 1.84 mg/ml for methanol and water extracts of S. nigrum and 1.09 and 4.9 mg/ml for C. gynandra, respectively. Conclusion: The vegetables, C. gynandra and S. nigrum are rich sources of diverse phytochemicals. However, some phytochemicals are present in high levels and might act as anti nutritional factors interfering with absorption of food. C. gynandra is safe for consumption but S. nigrum contains some toxic phytochemicals and should not be taken regularly.
{"title":"Phytochemical, Anti-nutrients and Toxicity Evaluation of Cleome gynandra and Solanum nigrum: Common Indigenous Vegetables in Zimbabwe","authors":"Caroline Sango, L. Marufu, C. Zimudzi","doi":"10.9734/BBJ/2016/25164","DOIUrl":"https://doi.org/10.9734/BBJ/2016/25164","url":null,"abstract":"Aim: This study assesses the phytochemical profiles, toxicity and anti-nutrient properties of two indigenous vegetables, Cleome gynandra and Solanum nigrum. Methodology: Aerial parts of S. nigrum and C. gynandra collected from wild populations were extracted in water and methanol solvents using the cold maceration procedure. Chemical tests to identify different phytochemicals and antinutrients were carried out using specified reagents. Toxicity of the plant extracts was tested using the Brine Shrimp (Artemia salina) lethality bioassay procedure. Results: Phytochemical tests indicated presence of alkaloids, steroids, flavonoids, cardiac glycosides, saponins, phenols and tannins in both S. nigrum and C. gynandra. Anthraquinones were only found in C. gynandra, whereas terpenoids and coumarin glycosides were found only in Original Research Article Sango et al.; BBJ, 13(3): 1-11, 2016; Article no.BBJ.25164 2 S. nigrum. The anti-nutrient levels in the vegetables were high; tannins (3.580 and 1.820 mg/ 100 g), oxalates (21.560 and 24.97±0.156 g/100 g), saponins (71 and 42%), alkaloids (15.160 and 3.44%) and phytates (0.099±0.046 and 0.250±0.013%) in S. nigrum and C. gynandra respectively. The Brine Shrimp LC50 values obtained were 0.67 and 1.84 mg/ml for methanol and water extracts of S. nigrum and 1.09 and 4.9 mg/ml for C. gynandra, respectively. Conclusion: The vegetables, C. gynandra and S. nigrum are rich sources of diverse phytochemicals. However, some phytochemicals are present in high levels and might act as anti nutritional factors interfering with absorption of food. C. gynandra is safe for consumption but S. nigrum contains some toxic phytochemicals and should not be taken regularly.","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"13 1","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71164619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Nakashima, K. Homma, Michiko Yamazaki, M. Ishizaki, K. Mawatari
Aims: To reveal which amino acid residues determine whether a protein is acidic or basic between orthologous pairs, acidic proteins from halophilic species and corresponding basic proteins from non-halophilic species were compared. Similarly acidic versus acidic protein pairs, and basic versus basic protein pairs were also analyzed. Place and Duration of Study: Department of Clinical Laboratory Science, Graduate Course of Medical Science and Technology, School of Health Sciences, Kanazawa University, Japan. Methodology: Halobacterium sp. NRC-1 was used as halophilic species and Gram-positive bacterium Bacillus subtilis, and radiation resistant bacterium Deinococcus radiodurans were used as non-halophilic species. The three species were selected because their proteins were closely Original Research Article Nakashima et al.; BBJ, 14(2): 1-12, 2016; Article no.BBJ.25207 2 related each other. The amino acid compositions were compared and the amino acid substitutions were counted for the orthologous protein pairs between Halobacterium and B. subtilis. Similar comparison was done for the proteins between Halobacterium and D. radiodurans. Results: The Asp and Glu residues are determinant whether a protein of Halobacterium sp. NRC-1 is acidic or basic. Amino acid substitutions to increase the Asp residues in the acidic proteins of Halobacterium from the corresponding proteins of non-halophilic species were almost identical whether the corresponding proteins were acidic or basic. This result suggested that the change of protein charges from basic proteins to acidic ones was same as from acidic proteins to acidic ones. The proteins of Halobacterium showed a tendency to have residues with smaller side chain than the proteins of B. subtilis / D. radiodurans.
{"title":"Database Analysis of Acidic Proteins from Halophilic Species and Their Corresponding Basic Proteins from Non-halophilic Species","authors":"H. Nakashima, K. Homma, Michiko Yamazaki, M. Ishizaki, K. Mawatari","doi":"10.9734/bbj/2016/25207","DOIUrl":"https://doi.org/10.9734/bbj/2016/25207","url":null,"abstract":"Aims: To reveal which amino acid residues determine whether a protein is acidic or basic between orthologous pairs, acidic proteins from halophilic species and corresponding basic proteins from non-halophilic species were compared. Similarly acidic versus acidic protein pairs, and basic versus basic protein pairs were also analyzed. Place and Duration of Study: Department of Clinical Laboratory Science, Graduate Course of Medical Science and Technology, School of Health Sciences, Kanazawa University, Japan. Methodology: Halobacterium sp. NRC-1 was used as halophilic species and Gram-positive bacterium Bacillus subtilis, and radiation resistant bacterium Deinococcus radiodurans were used as non-halophilic species. The three species were selected because their proteins were closely Original Research Article Nakashima et al.; BBJ, 14(2): 1-12, 2016; Article no.BBJ.25207 2 related each other. The amino acid compositions were compared and the amino acid substitutions were counted for the orthologous protein pairs between Halobacterium and B. subtilis. Similar comparison was done for the proteins between Halobacterium and D. radiodurans. Results: The Asp and Glu residues are determinant whether a protein of Halobacterium sp. NRC-1 is acidic or basic. Amino acid substitutions to increase the Asp residues in the acidic proteins of Halobacterium from the corresponding proteins of non-halophilic species were almost identical whether the corresponding proteins were acidic or basic. This result suggested that the change of protein charges from basic proteins to acidic ones was same as from acidic proteins to acidic ones. The proteins of Halobacterium showed a tendency to have residues with smaller side chain than the proteins of B. subtilis / D. radiodurans.","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"14 1","pages":"1-12"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71164681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Agbebi, C. Echefu, I. Adeosun, A. Ajibade, E. A. Adegbite, A. Adebambo, M. B. Ilori, So Durosaro, A. B. Ajibike
Aim: The aims are to study the genetic diversity and population structure of Tilapia fish species in Nigeria using mtDNA D-loop region and time divergence of these various groups of fish to give a baseline information for fish management and conservation programs. Methodology: Blood samples from four species of tilapia were collected with the use of FTA cards for DNA extraction and PCR amplification. A 941bp long fragment, including the D-loop, was subsequently used for analysis. Molecular diversity indices and Tajima’s selective test were determined. A phylogenetic tree was constructed for all the four fish species using UPGMA. The divergence time among the four fish species using MEGA5 software.
目的:研究尼日利亚罗非鱼种群的遗传多样性和种群结构，利用mtDNA d -环区和这些不同种群的时间散度，为鱼类管理和保护计划提供基线信息。方法:采用FTA卡采集4种罗非鱼的血样，进行DNA提取和PCR扩增。一个941bp长的片段，包括D-loop，随后被用于分析。测定分子多样性指数和田岛选择试验。利用UPGMA构建了4种鱼类的系统发育树。利用MEGA5软件计算四种鱼类的分化时间。
{"title":"Mitochondrial Diversity and Time Divergence of Commonly Cultured Cichlids in Nigeria","authors":"O. Agbebi, C. Echefu, I. Adeosun, A. Ajibade, E. A. Adegbite, A. Adebambo, M. B. Ilori, So Durosaro, A. B. Ajibike","doi":"10.9734/BBJ/2016/25470","DOIUrl":"https://doi.org/10.9734/BBJ/2016/25470","url":null,"abstract":"Aim: The aims are to study the genetic diversity and population structure of Tilapia fish species in Nigeria using mtDNA D-loop region and time divergence of these various groups of fish to give a baseline information for fish management and conservation programs. Methodology: Blood samples from four species of tilapia were collected with the use of FTA cards for DNA extraction and PCR amplification. A 941bp long fragment, including the D-loop, was subsequently used for analysis. Molecular diversity indices and Tajima’s selective test were determined. A phylogenetic tree was constructed for all the four fish species using UPGMA. The divergence time among the four fish species using MEGA5 software.","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"97 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71164756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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