Sujay Paul, Yolanda Cortez, Nadia Vera, G. K. Villena, M. Gutiérrez-Correa
{"title":"Metagenomic Analysis of Microbial Communities in the Soil-mousse Surrounding of an Amazonian Geothermal Spring in Peru","authors":"Sujay Paul, Yolanda Cortez, Nadia Vera, G. K. Villena, M. Gutiérrez-Correa","doi":"10.9734/BBJ/2016/27519","DOIUrl":"https://doi.org/10.9734/BBJ/2016/27519","url":null,"abstract":"","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"15 1","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71165012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aims: The study was carried out to determine the potentials of novel slow-release fertilizers (SRF) in the bioremediation of petroleum hydrocarbon-impacted soil, in order to determine their potentials in the bioremediation of petroleum-impacted sites and as well evaluate the effect of nutrient concentration on the rate of bioremediation. Study Design: A marine biopolymer (agar agar) was used as a coating for soluble NPK fertilizer in slow-release formulations (capsular and granular form). Place and Duration of Study: This study was carried out in the Environmental Microbiology Laboratory, University of Port Harcourt, Nigeria, between January and June 2015. Methodology: The contaminated soil sample was recreated in four clean plastic containers and labeled A D, as follows: Sample A = 300 g Soil + 20 g NPK Capsular SRF; Sample B = 300 g Soil + 20 g NPK Granular SRF; Sample C = 300 g Soil + 20 g Direct NPK; Sample D = 300 g Soil (without fertilizer control). The determination of the effect of SRF on the population dynamics of Original Research Article Sampson et al.; BBJ, 13(4): 1-13, 2016; Article no.BBJ.25955 2 total aerobic heterotrophic bacteria (THB) and hydrocarbon utilizing bacteria (HUB) was achieved through the use of nutrient agar (spread plate technique) and mineral salts agar (vapour phase transfer technique) in the enumeration of THB and HUB respectively. Results: After a 42-day period, there was a significant difference, (p <0 .05) in the percentage loss of total petroleum hydrocarbon between the various treatment options. Sample D had the least percentage loss (33.6%) of total petroleum hydrocarbon, Sample A (50.5%), Sample B (73.1%) and Sample C had the highest percentage loss of 74.83%. The various bacterial counts (THB and HUB) increased progressively with increase in nutrient concentration. Conclusion: The results revealed the applicability and effectiveness of slow release fertilizers in the bioremediation of hydrocarbon impacted soil. These novel SRFs are also recommended for their applicability in the bioremediation of water and sediments.
{"title":"Production and Application of Agar-based Slow-release Fertilizers, in the Bioremediation of Petroleum Hydrocarbon-impacted Soil","authors":"T. Sampson, C. Ogugbue, G. Okpokwasili","doi":"10.9734/bbj/2016/25955","DOIUrl":"https://doi.org/10.9734/bbj/2016/25955","url":null,"abstract":"Aims: The study was carried out to determine the potentials of novel slow-release fertilizers (SRF) in the bioremediation of petroleum hydrocarbon-impacted soil, in order to determine their potentials in the bioremediation of petroleum-impacted sites and as well evaluate the effect of nutrient concentration on the rate of bioremediation. Study Design: A marine biopolymer (agar agar) was used as a coating for soluble NPK fertilizer in slow-release formulations (capsular and granular form). Place and Duration of Study: This study was carried out in the Environmental Microbiology Laboratory, University of Port Harcourt, Nigeria, between January and June 2015. Methodology: The contaminated soil sample was recreated in four clean plastic containers and labeled A D, as follows: Sample A = 300 g Soil + 20 g NPK Capsular SRF; Sample B = 300 g Soil + 20 g NPK Granular SRF; Sample C = 300 g Soil + 20 g Direct NPK; Sample D = 300 g Soil (without fertilizer control). The determination of the effect of SRF on the population dynamics of Original Research Article Sampson et al.; BBJ, 13(4): 1-13, 2016; Article no.BBJ.25955 2 total aerobic heterotrophic bacteria (THB) and hydrocarbon utilizing bacteria (HUB) was achieved through the use of nutrient agar (spread plate technique) and mineral salts agar (vapour phase transfer technique) in the enumeration of THB and HUB respectively. Results: After a 42-day period, there was a significant difference, (p <0 .05) in the percentage loss of total petroleum hydrocarbon between the various treatment options. Sample D had the least percentage loss (33.6%) of total petroleum hydrocarbon, Sample A (50.5%), Sample B (73.1%) and Sample C had the highest percentage loss of 74.83%. The various bacterial counts (THB and HUB) increased progressively with increase in nutrient concentration. Conclusion: The results revealed the applicability and effectiveness of slow release fertilizers in the bioremediation of hydrocarbon impacted soil. These novel SRFs are also recommended for their applicability in the bioremediation of water and sediments.","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"13 1","pages":"1-13"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71165045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Farouk Segujja, B. Mwambi, Charles Drago, N. Lubowa, J. Mugambwa, Patrick Wabuyi
{"title":"Characterization and Antimicrobial Susceptibility Patterns of Isolates from Ward Fomites","authors":"Farouk Segujja, B. Mwambi, Charles Drago, N. Lubowa, J. Mugambwa, Patrick Wabuyi","doi":"10.9734/BBJ/2016/27861","DOIUrl":"https://doi.org/10.9734/BBJ/2016/27861","url":null,"abstract":"","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"14 1","pages":"1-16"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71165092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Bouyahya, N. Moussaoui, J. Abrini, Y. Bakri, N. Dakka
Aims: This study was designed to evaluate the antibacterial and antioxidant activities of methanolic, ethanolic, ethyl acetate and n-hexanic extract from the leaves of Strawberry tree (Arbutus unedo L.) growing in Morocco. Study Design: Determination of total phenol and flavonoids content, in vitro antioxidant activity and antibacterial study of extracts. Place and Duration of Study: Department of Biology (Faculty of Sciences), between July 2015 and November 2015. Original Research Article Bouyahya et al.; BBJ, 14(3): 1-10, 2016; Article no.BBJ.26488 2 Methodology: The extracts were prepared using solid-liquid extraction. The total phenolic content was assessed by the Folin-Ciocalteau assay, total flavonoid content was assessed by aluminium chloride (AlCl3) colorimetric assay. The antibacterial activity of extracts was tested against five reference strains, Escherichia coli K12 MBLA, Staphylococus aureus CECT 976, Listeria monocytogenes serovar 4b CECT 4032 and Pseudomonas aeruginosa IH using the agar well diffusion method and the micro-dilution assays. The antioxidant activity was assessed by measuring the ability of the extracts to scavenge the 2,2-diphenyl-1picrylhydrazyl (DPPH). Results: The total phenol content of strawberry tree five extracts ranged between 94.51±0.08 and 141.72±0.56 mg GAE/g extract, and the flavonoid content ranged between 17.48±1.02 and 31.61±0.59 mg QE/g extract. All of the extracts presented antioxidant capacity assessed by DPPH scavenging method, but at different levels depending on the concentration and the extraction solvent are found. In addition, all extracts exhibited an antibacterial activity at different levels against strains reported as the causal agents of food borne diseases. Conclusion: Results suggest the potential use of tested A. unedo extracts as bio-preservatives in the functional food industry.
{"title":"Determination of Phenolic Contents, Antioxidant and Antibacterial Activities of Strawberry Tree (Arbutus unedo L.) Leaf Extracts","authors":"A. Bouyahya, N. Moussaoui, J. Abrini, Y. Bakri, N. Dakka","doi":"10.9734/BBJ/2016/26488","DOIUrl":"https://doi.org/10.9734/BBJ/2016/26488","url":null,"abstract":"Aims: This study was designed to evaluate the antibacterial and antioxidant activities of methanolic, ethanolic, ethyl acetate and n-hexanic extract from the leaves of Strawberry tree (Arbutus unedo L.) growing in Morocco. Study Design: Determination of total phenol and flavonoids content, in vitro antioxidant activity and antibacterial study of extracts. Place and Duration of Study: Department of Biology (Faculty of Sciences), between July 2015 and November 2015. Original Research Article Bouyahya et al.; BBJ, 14(3): 1-10, 2016; Article no.BBJ.26488 2 Methodology: The extracts were prepared using solid-liquid extraction. The total phenolic content was assessed by the Folin-Ciocalteau assay, total flavonoid content was assessed by aluminium chloride (AlCl3) colorimetric assay. The antibacterial activity of extracts was tested against five reference strains, Escherichia coli K12 MBLA, Staphylococus aureus CECT 976, Listeria monocytogenes serovar 4b CECT 4032 and Pseudomonas aeruginosa IH using the agar well diffusion method and the micro-dilution assays. The antioxidant activity was assessed by measuring the ability of the extracts to scavenge the 2,2-diphenyl-1picrylhydrazyl (DPPH). Results: The total phenol content of strawberry tree five extracts ranged between 94.51±0.08 and 141.72±0.56 mg GAE/g extract, and the flavonoid content ranged between 17.48±1.02 and 31.61±0.59 mg QE/g extract. All of the extracts presented antioxidant capacity assessed by DPPH scavenging method, but at different levels depending on the concentration and the extraction solvent are found. In addition, all extracts exhibited an antibacterial activity at different levels against strains reported as the causal agents of food borne diseases. Conclusion: Results suggest the potential use of tested A. unedo extracts as bio-preservatives in the functional food industry.","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"14 1","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71165131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rosilma Araujo-Melo, I. Souza, Maria Vicalvi-Costa, J. M. Araújo, K. X. F. R. Sena, L. Coelho
{"title":"Actinobacteria: Versatile Microorganisms with Medical and Pharmaceutical Application","authors":"Rosilma Araujo-Melo, I. Souza, Maria Vicalvi-Costa, J. M. Araújo, K. X. F. R. Sena, L. Coelho","doi":"10.9734/BBJ/2016/28728","DOIUrl":"https://doi.org/10.9734/BBJ/2016/28728","url":null,"abstract":"","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"15 1","pages":"1-13"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71165512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Gomina, A. Alassani, L. Codjo, A. Dovonou, S. Akpona
{"title":"Prevalence of Dyslipidemia on the Basis of Biochemistry Tests in the Parakou University Teaching Hospital (Benin)","authors":"M. Gomina, A. Alassani, L. Codjo, A. Dovonou, S. Akpona","doi":"10.9734/BBJ/2016/28362","DOIUrl":"https://doi.org/10.9734/BBJ/2016/28362","url":null,"abstract":"","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"16 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71165814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Reproductive Characteristics of Rabbit Bucks Fed Diet Containing Raw or Fermented Cottonseed Cake","authors":"O. Amao, K. Showunmi","doi":"10.9734/bbj/2016/15383","DOIUrl":"https://doi.org/10.9734/bbj/2016/15383","url":null,"abstract":"","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"10 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71163908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"DNA from Oral Rinse- A Comparison of Three Protocols for Amplification of VDR, FTO and Beta-Globin Genes","authors":"M. Lucky, S. Baig","doi":"10.9734/bbj/2016/22199","DOIUrl":"https://doi.org/10.9734/bbj/2016/22199","url":null,"abstract":"","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"11 1","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71164031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. S. Akhtar, Fahad Siddiqui, A. Sheikh, S. Rashid, Z. Khurshid, S. Najeeb, Muhammad Sohail Zafar
{"title":"Frequency of Procedural Errors during Root Canal Treatment Performed by Interns","authors":"S. S. Akhtar, Fahad Siddiqui, A. Sheikh, S. Rashid, Z. Khurshid, S. Najeeb, Muhammad Sohail Zafar","doi":"10.9734/bbj/2016/23768","DOIUrl":"https://doi.org/10.9734/bbj/2016/23768","url":null,"abstract":"","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"12 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71164068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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