Lipases obtained from healthy and pebrinised larvae were purified by 45-85% (NH4) 2SO 4 fractionation followed by Sephadex S-100 gel filtration and CM-Sepharose. In both the samples final enzyme purification reported was 19.02 and 17.7 folds(magnitude) and the recovery of final purified enzyme was 19.32% and 17.14% with a specific activity of 7.87 and 7.52 µmol/min/mg. Results also show that activity of purified lipase was highest at pH 8 in both the samples. Highest lipase activity was recorded between 37°C to 40°C tem perature and lipase activity was maximum at 37°C temperature in healthy sample and 38°C tempe rature in pebrinised sample. The enzyme activity reduced with addition of NaCl, Urea and MgCl 2 whereas EDTA and CaCl 2 increased the activity. The molecular weight of the purified enzyme was 30 kDa as determined by SDSpolyacrylamide gel electrophoresis. Aim: The present study was conducted to isolate, purify and characterize lipases from the midgut of both healthy and pebrinised fifth instar larvae of Antheraea mylitta drury . Study Design: Study involves dissection of midgut from the fifth instar larvae of fourth day of both healthy and pebrinised larvae, Lipases obtained from both the samples were purified by 45-85%
{"title":"Isolation and Purification of Lipase from the Midgut of Fifth Instar Larvae of Antheraea mylitta drury","authors":"Lakshmi Marepally, G. Benarjee","doi":"10.9734/BBJ/2016/24442","DOIUrl":"https://doi.org/10.9734/BBJ/2016/24442","url":null,"abstract":"Lipases obtained from healthy and pebrinised larvae were purified by 45-85% (NH4) 2SO 4 fractionation followed by Sephadex S-100 gel filtration and CM-Sepharose. In both the samples final enzyme purification reported was 19.02 and 17.7 folds(magnitude) and the recovery of final purified enzyme was 19.32% and 17.14% with a specific activity of 7.87 and 7.52 µmol/min/mg. Results also show that activity of purified lipase was highest at pH 8 in both the samples. Highest lipase activity was recorded between 37°C to 40°C tem perature and lipase activity was maximum at 37°C temperature in healthy sample and 38°C tempe rature in pebrinised sample. The enzyme activity reduced with addition of NaCl, Urea and MgCl 2 whereas EDTA and CaCl 2 increased the activity. The molecular weight of the purified enzyme was 30 kDa as determined by SDSpolyacrylamide gel electrophoresis. Aim: The present study was conducted to isolate, purify and characterize lipases from the midgut of both healthy and pebrinised fifth instar larvae of Antheraea mylitta drury . Study Design: Study involves dissection of midgut from the fifth instar larvae of fourth day of both healthy and pebrinised larvae, Lipases obtained from both the samples were purified by 45-85%","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"12 1","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71164951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Antioxidant Property and Phenolic Content of Anthocleista vogelii Root Ethanolic Extract and Fractions","authors":"R. Sunday, O. Ilesanmi, E. Obuotor","doi":"10.9734/BBJ/2016/27016","DOIUrl":"https://doi.org/10.9734/BBJ/2016/27016","url":null,"abstract":"","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"14 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71165359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evaluation of Virulence of Tanzanian Strains of Fowlpox and Pigeonpox Viruses in Chickens","authors":"S. Masola, A. Mzula, C. Kasanga, P. Wambura","doi":"10.9734/bbj/2016/20150","DOIUrl":"https://doi.org/10.9734/bbj/2016/20150","url":null,"abstract":"","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"10 1","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71163614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aims: This research studied the regulatory effect of ABA (Abscisic acid) on the contents of lycopene and endogenous hperipheral pericarp of tomato fruit during ripening, and provided an effective measure for improving the quality of tomato fruits. Study Design: When the second cluster of toormone in mato fruits reached green mature stage, fruits were labeled and sprayed ABA (100 mg·L) over fruit surface, with spaying water as control, avoiding polluting the leaves. After treatment for 0, 4, 8, 12, 16, 20d, 10 fruits were sampled and dissected, peripheral pericarp for measuring the contents of lycopene, GA3 (Gibberellic acid), ABA, ZT (Zeatin) and ETH (Ethylene). Place and Duration of Study: Horticultural College, between February 2014 and March 2015. Methodology: The contents of lycopene, GA3, ABA and ZT were determined using HPLC (High Performance Liquid Chromatography). The content of ETH was determined using GC (Gas Chromatography). Results: The effect of exogenous ABA on the lycopene content in peripheral pericarp of tomato Short Research Article Yu et al.; BBJ, 16(1): 1-5, 2016; Article no.BBJ.28767 2 fruits was firstly inhibited then promoted during fruit mature period. Exogenous ABA decreased the content of endogenous GA3, but the endogenous ABA content was inhibited firstly, and then promoted, inhibited finally, and promoted the content decrease of endogenous ZT. The peak value was appeared early, but a little effect on the amount of ETH release. Conclusion: This work proved the feasibility of elevating of lycopene levels in tomato fruit by the application of exogenous ABA. ABA promoted the accumulation of lycopene by the decreasing of endogenous GA3, ZT and ABA contents, and by releasing peak appear of endogenous ETH.
{"title":"Effects of Exogenous ABA on Contents of Lycopene and Endogenous Hormone in Tomato Pericarp","authors":"Yang Yu, Q. Weng, Bao-li Zhou","doi":"10.9734/BBJ/2016/28767","DOIUrl":"https://doi.org/10.9734/BBJ/2016/28767","url":null,"abstract":"Aims: This research studied the regulatory effect of ABA (Abscisic acid) on the contents of lycopene and endogenous hperipheral pericarp of tomato fruit during ripening, and provided an effective measure for improving the quality of tomato fruits. Study Design: When the second cluster of toormone in mato fruits reached green mature stage, fruits were labeled and sprayed ABA (100 mg·L) over fruit surface, with spaying water as control, avoiding polluting the leaves. After treatment for 0, 4, 8, 12, 16, 20d, 10 fruits were sampled and dissected, peripheral pericarp for measuring the contents of lycopene, GA3 (Gibberellic acid), ABA, ZT (Zeatin) and ETH (Ethylene). Place and Duration of Study: Horticultural College, between February 2014 and March 2015. Methodology: The contents of lycopene, GA3, ABA and ZT were determined using HPLC (High Performance Liquid Chromatography). The content of ETH was determined using GC (Gas Chromatography). Results: The effect of exogenous ABA on the lycopene content in peripheral pericarp of tomato Short Research Article Yu et al.; BBJ, 16(1): 1-5, 2016; Article no.BBJ.28767 2 fruits was firstly inhibited then promoted during fruit mature period. Exogenous ABA decreased the content of endogenous GA3, but the endogenous ABA content was inhibited firstly, and then promoted, inhibited finally, and promoted the content decrease of endogenous ZT. The peak value was appeared early, but a little effect on the amount of ETH release. Conclusion: This work proved the feasibility of elevating of lycopene levels in tomato fruit by the application of exogenous ABA. ABA promoted the accumulation of lycopene by the decreasing of endogenous GA3, ZT and ABA contents, and by releasing peak appear of endogenous ETH.","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"16 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71165521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Impact of Probiotic Strain LBKV-3 on Phagocytic Cells of Children under Age of Ten","authors":"S. Hajare, T. Geremew","doi":"10.9734/BBJ/2016/26531","DOIUrl":"https://doi.org/10.9734/BBJ/2016/26531","url":null,"abstract":"","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"14 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71165204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Decolorization of Different Azo Dyes and Detoxification of Dyeing Wastewater by Pseudomonas stutzeri (SB_13) Isolated from Textile Dyes Effluent","authors":"A. Fouda, S. Hassan, M. S. Azab, Ebrahim Saied","doi":"10.9734/BBJ/2016/28363","DOIUrl":"https://doi.org/10.9734/BBJ/2016/28363","url":null,"abstract":"","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"15 1","pages":"1-18"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71165823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aims: To determine the influence of Moringa oleifera leaf meal (MOLM) on haematological and serum biochemical indices of growing rabbits fed diets containing varying levels of Moringa oleifera leaf meal. Study Design: Completely randomized design. Place and Duration of Study: At the Rabbit unit of the Teaching and Research farm of Federal College of Agriculture, Ishiagu, Ivo L.G.A., State, Nigeria, between May, 2015 and July 2015. Methodology: 48 growing rabbits were used for this study. Four diets were formulated such that diets T1, T2, T3 and T4 contain MOLM at 0%, 10%, 20% and 30%, respectively. The diets were offered to the growing rabbits, which were randomly divided into 4 groups of 12 rabbits each, with 4 animals constituting a replicate in a completely randomized design pattern. Each animal received the experimental diet for 49 days. Blood samples were drawn from each animal on the last day of Original Research Article Jiwuba et al.; BBJ, 15(2): 1-7, 2016; Article no.BBJ.28095 2 the trial and evaluated for haematological and serum biochemical indices, data obtained were analysed statistically. Results: Results showed that packed cell volume (PCV), haemoglobin Mean cell haemoglobin concentration and white blood cell differed (P<0.05) significantly, while red blood cell, mean cell haemoglobin and mean cell volume were similar (P>0.05) among the groups PCV ranged between 35.01–36.59%. Haemoglobin was improved (p<0.05) by Moringa oleifera leaf meal at 30% inclusion level. White blood cell (WBC) count of weaner rabbits in treatment groups was significantly (p<0.05) higher and better than the control. All the serum biochemical indices were significantly (P<0.05) except creatinine. Cholesterol was lowest at 30% inclusion indicating the antidiabetic properties of the test ingredient. Conclusions: All the parameters studied fell within the normal range reported for clinically healthy rabbits; an indication that MOLM had a beneficial effect on health status of rabbits.
目的:研究辣木叶粕(MOLM)对饲粮中添加不同水平辣木叶粕生长兔血液学和血清生化指标的影响。研究设计:完全随机设计。学习地点和时间:2015年5月至2015年7月，在尼日利亚Ivo L.G.A州Ishiagu联邦农业学院教学和研究农场的兔子单元。方法:选用生长兔48只。分别配制饲粮T1、T2、T3和T4中MOLM含量为0%、10%、20%和30%的4种饲粮。采用完全随机设计模式，将生长兔随机分为4组，每组12只，每组4只重复。每只动物饲喂49 d的试验饲粮。在原研究文章Jiwuba et al.的最后一天抽取每只动物的血液样本;植物学报，15(2):1-7,2016;文章no.BBJ。试验及评价血液学及血清生化指标，对所得数据进行统计学分析。结果:两组间细胞堆积体积(PCV)、血红蛋白、白细胞平均浓度(PCV)在35.01 ~ 36.59%之间，差异有统计学意义(P0.05)。30%辣木叶粕能显著提高血红蛋白(p<0.05)。各处理组断奶兔白细胞(WBC)计数显著(p<0.05)高于且优于对照组。除肌酐外，其余血清生化指标均显著(P<0.05)。胆固醇含量在30%时最低，表明测试成分具有抗糖尿病特性。结论:所研究的各项参数均在临床健康家兔的正常范围内;提示MOLM对家兔健康状况有有益影响。
{"title":"Haematological and Serum Biochemical Indices of Growing Rabbits Fed Diets Containing Varying Levels of Moringa oleifera Leaf Meal","authors":"P. Jiwuba, K. Ikwunze, E. Dauda, D. Ugwu","doi":"10.9734/BBJ/2016/28095","DOIUrl":"https://doi.org/10.9734/BBJ/2016/28095","url":null,"abstract":"Aims: To determine the influence of Moringa oleifera leaf meal (MOLM) on haematological and serum biochemical indices of growing rabbits fed diets containing varying levels of Moringa oleifera leaf meal. Study Design: Completely randomized design. Place and Duration of Study: At the Rabbit unit of the Teaching and Research farm of Federal College of Agriculture, Ishiagu, Ivo L.G.A., State, Nigeria, between May, 2015 and July 2015. Methodology: 48 growing rabbits were used for this study. Four diets were formulated such that diets T1, T2, T3 and T4 contain MOLM at 0%, 10%, 20% and 30%, respectively. The diets were offered to the growing rabbits, which were randomly divided into 4 groups of 12 rabbits each, with 4 animals constituting a replicate in a completely randomized design pattern. Each animal received the experimental diet for 49 days. Blood samples were drawn from each animal on the last day of Original Research Article Jiwuba et al.; BBJ, 15(2): 1-7, 2016; Article no.BBJ.28095 2 the trial and evaluated for haematological and serum biochemical indices, data obtained were analysed statistically. Results: Results showed that packed cell volume (PCV), haemoglobin Mean cell haemoglobin concentration and white blood cell differed (P<0.05) significantly, while red blood cell, mean cell haemoglobin and mean cell volume were similar (P>0.05) among the groups PCV ranged between 35.01–36.59%. Haemoglobin was improved (p<0.05) by Moringa oleifera leaf meal at 30% inclusion level. White blood cell (WBC) count of weaner rabbits in treatment groups was significantly (p<0.05) higher and better than the control. All the serum biochemical indices were significantly (P<0.05) except creatinine. Cholesterol was lowest at 30% inclusion indicating the antidiabetic properties of the test ingredient. Conclusions: All the parameters studied fell within the normal range reported for clinically healthy rabbits; an indication that MOLM had a beneficial effect on health status of rabbits.","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"54 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71165953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kishor Kumar Sarker, F. Monshi, Sayeda Sultana, Delara Akhter, M. Bhuiyan
{"title":"Development of an Efficient Plant Regeneration System of Field Mustard (Brassica campestris)","authors":"Kishor Kumar Sarker, F. Monshi, Sayeda Sultana, Delara Akhter, M. Bhuiyan","doi":"10.9734/bbj/2016/21529","DOIUrl":"https://doi.org/10.9734/bbj/2016/21529","url":null,"abstract":"","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"12 1","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71163823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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